AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046464.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046464.01.T01 MTR_4g108700 91.228 57 5 0 1 57 25 81 7.57e-33 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAACTTTGAGGTCTTAAA+TGG 0.146569 8:+73671524 None:intergenic
TTGTTTATGTATGGGATCTT+AGG 0.200401 8:-73670156 MsG0880046464.01.T01:three_prime_UTR
TCCATCCTCTCATTAATTCT+TGG 0.236814 8:+73670373 None:intergenic
CTCTCATTAATTCTTGGCTT+TGG 0.279055 8:+73670379 None:intergenic
AATTAATGAGAGGATGGATT+TGG 0.333806 8:-73670368 MsG0880046464.01.T01:CDS
AAATCCCCATTGTTTATGTA+TGG 0.334660 8:-73670165 MsG0880046464.01.T01:three_prime_UTR
TCTCCATTGTGGATGCTCTT+AGG 0.355519 8:-73671499 MsG0880046464.01.T01:five_prime_UTR
CTCAATGATTATGCACCATC+TGG 0.362640 8:-73670343 MsG0880046464.01.T01:CDS
AGAGGAACTAACATTTAATC+AGG 0.392881 8:+73670276 None:intergenic
CAACTTGGACATCTTGCATA+AGG 0.415467 8:+73670572 None:intergenic
CCGGAGTAATTAGGTCAACT+TGG 0.423783 8:+73670557 None:intergenic
AATCAGGTTACACACCATTA+CGG 0.442297 8:+73670292 None:intergenic
AGATCCCATACATAAACAAT+GGG 0.456560 8:+73670160 None:intergenic
ACACACCATTACGGTGCTCT+TGG 0.466740 8:+73670301 None:intergenic
TATGCACCATCTGGGGCAAA+TGG 0.473723 8:-73670334 MsG0880046464.01.T01:CDS
AATGGAGACCCTCAACTTTG+AGG 0.476203 8:+73671514 None:intergenic
ATCATTGAAATTCTAGTACA+TGG 0.502495 8:+73670098 None:intergenic
ATTTAAGACCTCAAAGTTGA+GGG 0.509562 8:-73671522 MsG0880046464.01.T01:five_prime_UTR
AATCCCCATTGTTTATGTAT+GGG 0.510722 8:-73670164 MsG0880046464.01.T01:three_prime_UTR
TCAATGATTATGCACCATCT+GGG 0.530735 8:-73670342 MsG0880046464.01.T01:CDS
ATTTATGTATGATGAAAGAA+AGG 0.544291 8:-73670208 MsG0880046464.01.T01:three_prime_UTR
TCAACTTTGAGGTCTTAAAT+GGG 0.544504 8:+73671525 None:intergenic
GCCAAGAATTAATGAGAGGA+TGG 0.546052 8:-73670374 MsG0880046464.01.T01:CDS
AAGATCCCATACATAAACAA+TGG 0.554557 8:+73670159 None:intergenic
TACTCCGGAAAATACCCCTC+AGG 0.579089 8:-73670542 MsG0880046464.01.T01:intron
ACACTCCAAGAGCACCGTAA+TGG 0.580515 8:-73670306 MsG0880046464.01.T01:exon
CATTTAAGACCTCAAAGTTG+AGG 0.586490 8:-73671523 MsG0880046464.01.T01:five_prime_UTR
CCAAGTTGACCTAATTACTC+CGG 0.594810 8:-73670557 MsG0880046464.01.T01:CDS
CAAAGCCAAGAATTAATGAG+AGG 0.628754 8:-73670378 MsG0880046464.01.T01:CDS
TGACGACCATTTGCCCCAGA+TGG 0.633835 8:+73670328 None:intergenic
CAATGATTATGCACCATCTG+GGG 0.636327 8:-73670341 MsG0880046464.01.T01:CDS
AAAGTTGAGGGTCTCCATTG+TGG 0.650229 8:-73671510 MsG0880046464.01.T01:five_prime_UTR
TTTCCTAAGAGCATCCACAA+TGG 0.676299 8:+73671496 None:intergenic
GATCCCATACATAAACAATG+GGG 0.744720 8:+73670161 None:intergenic
AGTATCAAACTGTACCTGAG+GGG 0.746962 8:+73670528 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGAAATACTATTAATT+TGG - Chr8:73670421-73670440 MsG0880046464.01.T01:intron 10.0%
!! ATATGAAATACTATTAATTT+GGG - Chr8:73670422-73670441 MsG0880046464.01.T01:intron 10.0%
!!! ATTAATAGTATTTCATATTT+TGG + Chr8:73670420-73670439 None:intergenic 10.0%
!! AAAAAAGTTAGTTGAAACTT+TGG - Chr8:73670388-73670407 MsG0880046464.01.T01:CDS 20.0%
!! AACAAGATTATATATGTTAC+AGG - Chr8:73671183-73671202 MsG0880046464.01.T01:intron 20.0%
!! ATTTATGTATGATGAAAGAA+AGG - Chr8:73671409-73671428 MsG0880046464.01.T01:intron 20.0%
!! CTTAATTTACATTATTGTTG+TGG - Chr8:73670262-73670281 MsG0880046464.01.T01:three_prime_UTR 20.0%
!!! ATATATGAGTTTTATTGTAG+AGG - Chr8:73671385-73671404 MsG0880046464.01.T01:intron 20.0%
!!! CTTAATATTGTTTTACATAG+AGG + Chr8:73671362-73671381 None:intergenic 20.0%
!!! TGGTATATTTTAAGTCAATA+TGG + Chr8:73670199-73670218 None:intergenic 20.0%
!!! TTTGTGATGTTTTTTTAACA+TGG + Chr8:73670590-73670609 None:intergenic 20.0%
! AAAGTTTATTTACCAAAGTG+TGG - Chr8:73670476-73670495 MsG0880046464.01.T01:intron 25.0%
! ATAAATTGAGAACTAGCATA+TGG - Chr8:73670626-73670645 MsG0880046464.01.T01:intron 25.0%
! ATCATTGAAATTCTAGTACA+TGG + Chr8:73671522-73671541 None:intergenic 25.0%
! TAAATTGAGAACTAGCATAT+GGG - Chr8:73670627-73670646 MsG0880046464.01.T01:intron 25.0%
! TACGTCTATATTTGAATTGA+CGG - Chr8:73670829-73670848 MsG0880046464.01.T01:intron 25.0%
! TAGAAGAATTTACTTGTAAC+TGG + Chr8:73670776-73670795 None:intergenic 25.0%
! TTTAGGTGGACATAAAATTA+TGG + Chr8:73670219-73670238 None:intergenic 25.0%
!! AGTCTTAGTCTTCTTATATA+TGG - Chr8:73670994-73671013 MsG0880046464.01.T01:intron 25.0%
!! CACACAATTCTGATATTTTA+TGG + Chr8:73670355-73670374 None:intergenic 25.0%
AAATCCCCATTGTTTATGTA+TGG - Chr8:73671452-73671471 MsG0880046464.01.T01:intron 30.0%
AAGATCCCATACATAAACAA+TGG + Chr8:73671461-73671480 None:intergenic 30.0%
AATCCCCATTGTTTATGTAT+GGG - Chr8:73671453-73671472 MsG0880046464.01.T01:intron 30.0%
AGAGGAACTAACATTTAATC+AGG + Chr8:73671344-73671363 None:intergenic 30.0%
AGATCCCATACATAAACAAT+GGG + Chr8:73671460-73671479 None:intergenic 30.0%
ATGGTATGTTTGTATTAAGC+AGG - Chr8:73671013-73671032 MsG0880046464.01.T01:intron 30.0%
ATTTGAATTGACGGTGAATT+TGG - Chr8:73670838-73670857 MsG0880046464.01.T01:intron 30.0%
CGATGAATTTCATGAAATCA+TGG + Chr8:73670936-73670955 None:intergenic 30.0%
GGAAAATTACATGCATGTTT+CGG + Chr8:73670963-73670982 None:intergenic 30.0%
TATTGAGAAAAACTTGCACT+TGG - Chr8:73671210-73671229 MsG0880046464.01.T01:intron 30.0%
TGAATTTCATGAAATCATGG+TGG + Chr8:73670933-73670952 None:intergenic 30.0%
TGCTATCAACTTTATGACTT+TGG - Chr8:73670530-73670549 MsG0880046464.01.T01:intron 30.0%
TTAAGCATAGAAGATGTGAA+AGG + Chr8:73670745-73670764 None:intergenic 30.0%
TTGTTTATGTATGGGATCTT+AGG - Chr8:73671461-73671480 MsG0880046464.01.T01:intron 30.0%
! AATTAATGAGAGGATGGATT+TGG - Chr8:73671249-73671268 MsG0880046464.01.T01:intron 30.0%
! ATTTAAGACCTCAAAGTTGA+GGG - Chr8:73670095-73670114 MsG0880046464.01.T01:three_prime_UTR 30.0%
!! ATTAATTTGGGTTTTCACAC+AGG - Chr8:73670434-73670453 MsG0880046464.01.T01:intron 30.0%
!! TCAACTTTGAGGTCTTAAAT+GGG + Chr8:73670095-73670114 None:intergenic 30.0%
!! TTTTAAGTCAATATGGAGTG+CGG + Chr8:73670192-73670211 None:intergenic 30.0%
AACATGGAATGTCGATATGA+TGG + Chr8:73670574-73670593 None:intergenic 35.0%
AATCAGGTTACACACCATTA+CGG + Chr8:73671328-73671347 None:intergenic 35.0%
ACTTTCTCTGCACTACAATA+AGG - Chr8:73670238-73670257 MsG0880046464.01.T01:three_prime_UTR 35.0%
ATAGCAACCCATCATTTCTT+GGG + Chr8:73670516-73670535 None:intergenic 35.0%
ATGAAAGTGGAAAGATGACA+AGG + Chr8:73670168-73670187 None:intergenic 35.0%
CAAAGCCAAGAATTAATGAG+AGG - Chr8:73671239-73671258 MsG0880046464.01.T01:intron 35.0%
CGTTTATTCGTGATTCTTCT+TGG - Chr8:73670719-73670738 MsG0880046464.01.T01:intron 35.0%
CTCTCATTAATTCTTGGCTT+TGG + Chr8:73671241-73671260 None:intergenic 35.0%
GACTAAGACTAAGAAGATGA+AGG + Chr8:73670984-73671003 None:intergenic 35.0%
GATCCCATACATAAACAATG+GGG + Chr8:73671459-73671478 None:intergenic 35.0%
TCAATGATTATGCACCATCT+GGG - Chr8:73671275-73671294 MsG0880046464.01.T01:intron 35.0%
TCCATCCTCTCATTAATTCT+TGG + Chr8:73671247-73671266 None:intergenic 35.0%
TTGTAGTGCAGAGAAAGTTT+AGG + Chr8:73670236-73670255 None:intergenic 35.0%
TTTGTCTCCCAAGAAATGAT+GGG - Chr8:73670506-73670525 MsG0880046464.01.T01:intron 35.0%
! CATTTAAGACCTCAAAGTTG+AGG - Chr8:73670094-73670113 MsG0880046464.01.T01:three_prime_UTR 35.0%
!! CTCAACTTTGAGGTCTTAAA+TGG + Chr8:73670096-73670115 None:intergenic 35.0%
ACAAACGTGTTTCCACACTT+TGG + Chr8:73670491-73670510 None:intergenic 40.0%
AGTATCAAACTGTACCTGAG+GGG + Chr8:73671092-73671111 None:intergenic 40.0%
ATTGAGAACTAGCATATGGG+AGG - Chr8:73670630-73670649 MsG0880046464.01.T01:intron 40.0%
CAACTTGGACATCTTGCATA+AGG + Chr8:73671048-73671067 None:intergenic 40.0%
CAATGATTATGCACCATCTG+GGG - Chr8:73671276-73671295 MsG0880046464.01.T01:intron 40.0%
CTCAATGATTATGCACCATC+TGG - Chr8:73671274-73671293 MsG0880046464.01.T01:intron 40.0%
GAGTATCAAACTGTACCTGA+GGG + Chr8:73671093-73671112 None:intergenic 40.0%
GATAGCAACCCATCATTTCT+TGG + Chr8:73670517-73670536 None:intergenic 40.0%
GCCAAGAATTAATGAGAGGA+TGG - Chr8:73671243-73671262 MsG0880046464.01.T01:intron 40.0%
GTTTGTCTCCCAAGAAATGA+TGG - Chr8:73670505-73670524 MsG0880046464.01.T01:intron 40.0%
TAGTGCAGAGAAAGTTTAGG+TGG + Chr8:73670233-73670252 None:intergenic 40.0%
TCTTCTCAGAAACAAGCTCT+AGG + Chr8:73670664-73670683 None:intergenic 40.0%
TGAATTGACGGTGAATTTGG+TGG - Chr8:73670841-73670860 MsG0880046464.01.T01:intron 40.0%
! AGAGTATCAAACTGTACCTG+AGG + Chr8:73671094-73671113 None:intergenic 40.0%
! CCAAGTTGACCTAATTACTC+CGG - Chr8:73671060-73671079 MsG0880046464.01.T01:intron 40.0%
! GGGTATTTTCCGGAGTAATT+AGG + Chr8:73671072-73671091 None:intergenic 40.0%
!! TTTCCTAAGAGCATCCACAA+TGG + Chr8:73670124-73670143 None:intergenic 40.0%
AAAGTTGAGGGTCTCCATTG+TGG - Chr8:73670107-73670126 MsG0880046464.01.T01:three_prime_UTR 45.0%
AATGGAGACCCTCAACTTTG+AGG + Chr8:73670106-73670125 None:intergenic 45.0%
CCGGAGTAATTAGGTCAACT+TGG + Chr8:73671063-73671082 None:intergenic 45.0%
TATGGAGTGCGGTATGAAAG+TGG + Chr8:73670181-73670200 None:intergenic 45.0%
TCTCCATTGTGGATGCTCTT+AGG - Chr8:73670118-73670137 MsG0880046464.01.T01:three_prime_UTR 45.0%
!!! TGTACCTGAGGGGTATTTTC+CGG + Chr8:73671082-73671101 None:intergenic 45.0%
ACACACCATTACGGTGCTCT+TGG + Chr8:73671319-73671338 None:intergenic 50.0%
TACTCCGGAAAATACCCCTC+AGG - Chr8:73671075-73671094 MsG0880046464.01.T01:intron 50.0%
! TATGCACCATCTGGGGCAAA+TGG - Chr8:73671283-73671302 MsG0880046464.01.T01:intron 50.0%
!! ACACTCCAAGAGCACCGTAA+TGG - Chr8:73671311-73671330 MsG0880046464.01.T01:intron 50.0%
CATTGCAGTGCAGGAATGGC+AGG - Chr8:73670686-73670705 MsG0880046464.01.T01:intron 55.0%
CTGCCATTCCTGCACTGCAA+TGG + Chr8:73670688-73670707 None:intergenic 55.0%
GAGAAGAGCCATTGCAGTGC+AGG - Chr8:73670677-73670696 MsG0880046464.01.T01:intron 55.0%
GAGCCATTGCAGTGCAGGAA+TGG - Chr8:73670682-73670701 MsG0880046464.01.T01:intron 55.0%
TGACGACCATTTGCCCCAGA+TGG + Chr8:73671292-73671311 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 73670084 73671555 73670084 ID=MsG0880046464.01;Name=MsG0880046464.01
Chr8 mRNA 73670084 73671555 73670084 ID=MsG0880046464.01.T01;Parent=MsG0880046464.01;Name=MsG0880046464.01.T01;_AED=0.54;_eAED=0.54;_QI=99|0.5|0.66|0.66|0.5|0.66|3|225|57
Chr8 exon 73670084 73670434 73670084 ID=MsG0880046464.01.T01:exon:7992;Parent=MsG0880046464.01.T01
Chr8 exon 73670543 73670604 73670543 ID=MsG0880046464.01.T01:exon:7991;Parent=MsG0880046464.01.T01
Chr8 exon 73671471 73671555 73671471 ID=MsG0880046464.01.T01:exon:7990;Parent=MsG0880046464.01.T01
Chr8 five_prime_UTR 73671471 73671555 73671471 ID=MsG0880046464.01.T01:five_prime_utr;Parent=MsG0880046464.01.T01
Chr8 five_prime_UTR 73670591 73670604 73670591 ID=MsG0880046464.01.T01:five_prime_utr;Parent=MsG0880046464.01.T01
Chr8 CDS 73670543 73670590 73670543 ID=MsG0880046464.01.T01:cds;Parent=MsG0880046464.01.T01
Chr8 CDS 73670309 73670434 73670309 ID=MsG0880046464.01.T01:cds;Parent=MsG0880046464.01.T01
Chr8 three_prime_UTR 73670084 73670308 73670084 ID=MsG0880046464.01.T01:three_prime_utr;Parent=MsG0880046464.01.T01
Gene Sequence

>MsG0880046464.01.T01

ATGCAAGATGTCCAAGTTGACCTAATTACTCCGGAAAATACCCCTCAGGTTATTATTGAGAAAAACTTGCACTTGGAAGAACCAAAGCCAAGAATTAATGAGAGGATGGATTTGGAACTCAATGATTATGCACCATCTGGGGCAAATGGTCGTCACACTCCAAGAGCACCGTAA

Protein sequence

>MsG0880046464.01.T01

MQDVQVDLITPENTPQVIIEKNLHLEEPKPRINERMDLELNDYAPSGANGRHTPRAP*