AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0052580


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0052580 MtrunA17_Chr1g0152461 91.438 292 17 2 1 291 1 285 0.0 536
Msa0052580 MtrunA17_Chr3g0134881 42.241 232 97 8 1 207 1 220 1.69e-46 156
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0052580 AT1G59590.1 39.683 189 103 5 22 202 7 192 1.65e-32 119
Msa0052580 AT1G10220.1 40.000 180 95 5 22 190 3 180 1.60e-25 101
Msa0052580 AT1G10220.2 37.696 191 95 5 22 190 3 191 5.45e-24 97.4

Find 41 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGGCCACGTGTGGAATA+AGG 0.226117 1_2:+6879125 None:intergenic
ATTTGTTGTGATAATGATTT+TGG 0.226913 1_2:-6878787 Msa0052580:CDS
ATTCCATGAAATTCTCTTCT+TGG 0.239938 1_2:+6878851 None:intergenic
AAGAAAAGAGATTATGTTAG+AGG 0.255949 1_2:-6878663 Msa0052580:CDS
TATCTGATTTAAATGATAAA+AGG 0.270978 1_2:-6879085 Msa0052580:CDS
AGGTTTGAGTGAGAAGAAAA+TGG 0.283239 1_2:-6878643 Msa0052580:CDS
CATAGTTCATCATCATCTTC+TGG 0.290163 1_2:+6879206 None:intergenic
TATCTTCTAGGGCTTGAATT+AGG 0.299114 1_2:+6879173 None:intergenic
TCAAAATCACCACCACTTCT+TGG 0.326218 1_2:+6878441 None:intergenic
GACTTAGGACTCAAGCTTCT+TGG 0.327238 1_2:+6878933 None:intergenic
TTCCATGAAATTCTCTTCTT+GGG 0.358637 1_2:+6878852 None:intergenic
CCCTTATTCCACACGTGGCC+TGG 0.364320 1_2:-6879126 Msa0052580:CDS
ATGAAATTCTCTTCTTGGGT+TGG 0.385407 1_2:+6878856 None:intergenic
GGGTTGGTTTCTTTCTTCCA+TGG 0.388895 1_2:+6878872 None:intergenic
AAGATGGAGTGAGAAGAAAA+TGG 0.393067 1_2:-6878490 Msa0052580:CDS
TTGAATGTTCCACAAACAAA+AGG 0.402250 1_2:+6879230 None:intergenic
CCAGGCCACGTGTGGAATAA+GGG 0.414462 1_2:+6879126 None:intergenic
GATTTAATATCCTTCAAAGT+TGG 0.427450 1_2:+6878699 None:intergenic
AAAATCATTATCACAACAAA+TGG 0.436688 1_2:+6878789 None:intergenic
AACCCAAGAAGAGAATTTCA+TGG 0.440286 1_2:-6878854 Msa0052580:CDS
AGAAAGATTATGTTAGAAGA+TGG 0.449967 1_2:-6878506 Msa0052580:CDS
AACATTCACACAAGAAAAGT+TGG 0.459312 1_2:-6878389 Msa0052580:CDS
AAAATGGTTACTACTAATGA+TGG 0.460282 1_2:-6878474 Msa0052580:CDS
GTATGTTATTCCAACTTTGA+AGG 0.503391 1_2:-6878709 Msa0052580:CDS
ATGGATGTAGTGAATAATGA+CGG 0.534632 1_2:-6878624 Msa0052580:CDS
TGAATGTTCCACAAACAAAA+GGG 0.542425 1_2:+6879231 None:intergenic
AAAGTGCTGAAAATTTATCC+AGG 0.555876 1_2:+6879108 None:intergenic
AAATTTATCCAGGCCACGTG+TGG 0.580635 1_2:+6879118 None:intergenic
GGATTAGTTTCTCCAAGAAG+TGG 0.582854 1_2:-6878453 Msa0052580:CDS
TGGATGTAGTGAATAATGAC+GGG 0.588904 1_2:-6878623 Msa0052580:CDS
AAAAGATGGTGGTTAATGAA+GGG 0.592112 1_2:-6878539 Msa0052580:CDS
GAATATGAAGAATATTGAGA+TGG 0.602572 1_2:-6878832 Msa0052580:CDS
TGAATCATCAATTGTTGCCA+TGG 0.622110 1_2:-6878889 Msa0052580:CDS
CGAACAAAAGAAATATCATG+AGG 0.627048 1_2:+6879038 None:intergenic
AAGCTTGAGTCCTAAGTCAA+CGG 0.627738 1_2:-6878928 Msa0052580:CDS
TTAGTTTCTCCAAGAAGTGG+TGG 0.628810 1_2:-6878450 Msa0052580:CDS
ACAAATGGCAAATAGAACAA+TGG 0.651573 1_2:+6878804 None:intergenic
GAACAAAAGAAATATCATGA+GGG 0.654561 1_2:+6879039 None:intergenic
AATAACCCTTATTCCACACG+TGG 0.697457 1_2:-6879131 Msa0052580:CDS
TGTAGTGAATAATGACGGGG+TGG 0.710681 1_2:-6878619 Msa0052580:CDS
GGATGTAGTGAATAATGACG+GGG 0.789564 1_2:-6878622 Msa0052580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTGATTTAAATGATAAA+AGG - chr1_2:6878540-6878559 Msa0052580:CDS 15.0%
!! TATCTGATTTAAATGATAAA+AGG - chr1_2:6878540-6878559 Msa0052580:CDS 15.0%
!! AAAATCATTATCACAACAAA+TGG + chr1_2:6878839-6878858 None:intergenic 20.0%
!!! ATTTGTTGTGATAATGATTT+TGG - chr1_2:6878838-6878857 Msa0052580:CDS 20.0%
!! AAGAAAAAAGATTATGTTAG+AGG - chr1_2:6879052-6879071 Msa0052580:CDS 20.0%
!! AGAAAAAAGATTATGTTAGA+GGG - chr1_2:6879053-6879072 Msa0052580:CDS 20.0%
!! AAAATCATTATCACAACAAA+TGG + chr1_2:6878839-6878858 None:intergenic 20.0%
!!! ATTTGTTGTGATAATGATTT+TGG - chr1_2:6878838-6878857 Msa0052580:CDS 20.0%
!! AAGAAAAAAGATTATGTTAG+AGG - chr1_2:6879052-6879071 Msa0052580:CDS 20.0%
!! AGAAAAAAGATTATGTTAGA+GGG - chr1_2:6879053-6879072 Msa0052580:CDS 20.0%
!! CTTTTCTTTTGTATCTTCTA+GGG + chr1_2:6878466-6878485 None:intergenic 25.0%
!! TCTTTTCTTTTGTATCTTCT+AGG + chr1_2:6878467-6878486 None:intergenic 25.0%
! GAACAAAAGAAATATCATGA+GGG + chr1_2:6878589-6878608 None:intergenic 25.0%
!! TTTTCACCTTAACAACAATA+GGG + chr1_2:6878643-6878662 None:intergenic 25.0%
!! TTTTTCACCTTAACAACAAT+AGG + chr1_2:6878644-6878663 None:intergenic 25.0%
! GAATATGAAGAATATTGAGA+TGG - chr1_2:6878793-6878812 Msa0052580:CDS 25.0%
!! TAATTTTCACTTGTATTCTG+TGG - chr1_2:6878894-6878913 Msa0052580:CDS 25.0%
! GATTTAATATCCTTCAAAGT+TGG + chr1_2:6878929-6878948 None:intergenic 25.0%
! AAGAAAAGAGATTATGTTAG+AGG - chr1_2:6878962-6878981 Msa0052580:CDS 25.0%
! AGAAAGATTATGTTAGAAGA+TGG - chr1_2:6879119-6879138 Msa0052580:CDS 25.0%
! AAAATGGTTACTACTAATGA+TGG - chr1_2:6879151-6879170 Msa0052580:CDS 25.0%
!! CTTTTCTTTTGTATCTTCTA+GGG + chr1_2:6878466-6878485 None:intergenic 25.0%
!! TCTTTTCTTTTGTATCTTCT+AGG + chr1_2:6878467-6878486 None:intergenic 25.0%
! GAACAAAAGAAATATCATGA+GGG + chr1_2:6878589-6878608 None:intergenic 25.0%
!! TTTTCACCTTAACAACAATA+GGG + chr1_2:6878643-6878662 None:intergenic 25.0%
!! TTTTTCACCTTAACAACAAT+AGG + chr1_2:6878644-6878663 None:intergenic 25.0%
! GAATATGAAGAATATTGAGA+TGG - chr1_2:6878793-6878812 Msa0052580:CDS 25.0%
!! TAATTTTCACTTGTATTCTG+TGG - chr1_2:6878894-6878913 Msa0052580:CDS 25.0%
! GATTTAATATCCTTCAAAGT+TGG + chr1_2:6878929-6878948 None:intergenic 25.0%
! AAGAAAAGAGATTATGTTAG+AGG - chr1_2:6878962-6878981 Msa0052580:CDS 25.0%
! AGAAAGATTATGTTAGAAGA+TGG - chr1_2:6879119-6879138 Msa0052580:CDS 25.0%
! AAAATGGTTACTACTAATGA+TGG - chr1_2:6879151-6879170 Msa0052580:CDS 25.0%
TGAATGTTCCACAAACAAAA+GGG + chr1_2:6878397-6878416 None:intergenic 30.0%
TTGAATGTTCCACAAACAAA+AGG + chr1_2:6878398-6878417 None:intergenic 30.0%
!! AAAGTGCTGAAAATTTATCC+AGG + chr1_2:6878520-6878539 None:intergenic 30.0%
CGAACAAAAGAAATATCATG+AGG + chr1_2:6878590-6878609 None:intergenic 30.0%
!! TTTTGTCCCTATTGTTGTTA+AGG - chr1_2:6878634-6878653 Msa0052580:CDS 30.0%
!! GTTTCACTACTTTTAACTCT+TGG + chr1_2:6878673-6878692 None:intergenic 30.0%
TTCCATGAAATTCTCTTCTT+GGG + chr1_2:6878776-6878795 None:intergenic 30.0%
ATTCCATGAAATTCTCTTCT+TGG + chr1_2:6878777-6878796 None:intergenic 30.0%
ACAAATGGCAAATAGAACAA+TGG + chr1_2:6878824-6878843 None:intergenic 30.0%
GTATGTTATTCCAACTTTGA+AGG - chr1_2:6878916-6878935 Msa0052580:CDS 30.0%
ATGGATGTAGTGAATAATGA+CGG - chr1_2:6879001-6879020 Msa0052580:CDS 30.0%
AGGGTTTAGTGAAAAAAAGA+TGG - chr1_2:6879072-6879091 Msa0052580:CDS 30.0%
GTTTAGTGAAAAAAAGATGG+TGG - chr1_2:6879075-6879094 Msa0052580:CDS 30.0%
! AAAAAGATGGTGGTTAATGA+AGG - chr1_2:6879085-6879104 Msa0052580:CDS 30.0%
! AAAAGATGGTGGTTAATGAA+GGG - chr1_2:6879086-6879105 Msa0052580:CDS 30.0%
! TTTTGCTTGAATCTTGTTCT+TGG + chr1_2:6879218-6879237 None:intergenic 30.0%
AACATTCACACAAGAAAAGT+TGG - chr1_2:6879236-6879255 Msa0052580:CDS 30.0%
TGAATGTTCCACAAACAAAA+GGG + chr1_2:6878397-6878416 None:intergenic 30.0%
TTGAATGTTCCACAAACAAA+AGG + chr1_2:6878398-6878417 None:intergenic 30.0%
!! AAAGTGCTGAAAATTTATCC+AGG + chr1_2:6878520-6878539 None:intergenic 30.0%
CGAACAAAAGAAATATCATG+AGG + chr1_2:6878590-6878609 None:intergenic 30.0%
!! TTTTGTCCCTATTGTTGTTA+AGG - chr1_2:6878634-6878653 Msa0052580:CDS 30.0%
!! GTTTCACTACTTTTAACTCT+TGG + chr1_2:6878673-6878692 None:intergenic 30.0%
TTCCATGAAATTCTCTTCTT+GGG + chr1_2:6878776-6878795 None:intergenic 30.0%
ATTCCATGAAATTCTCTTCT+TGG + chr1_2:6878777-6878796 None:intergenic 30.0%
ACAAATGGCAAATAGAACAA+TGG + chr1_2:6878824-6878843 None:intergenic 30.0%
GTATGTTATTCCAACTTTGA+AGG - chr1_2:6878916-6878935 Msa0052580:CDS 30.0%
ATGGATGTAGTGAATAATGA+CGG - chr1_2:6879001-6879020 Msa0052580:CDS 30.0%
AGGGTTTAGTGAAAAAAAGA+TGG - chr1_2:6879072-6879091 Msa0052580:CDS 30.0%
GTTTAGTGAAAAAAAGATGG+TGG - chr1_2:6879075-6879094 Msa0052580:CDS 30.0%
! AAAAAGATGGTGGTTAATGA+AGG - chr1_2:6879085-6879104 Msa0052580:CDS 30.0%
! AAAAGATGGTGGTTAATGAA+GGG - chr1_2:6879086-6879105 Msa0052580:CDS 30.0%
! TTTTGCTTGAATCTTGTTCT+TGG + chr1_2:6879218-6879237 None:intergenic 30.0%
AACATTCACACAAGAAAAGT+TGG - chr1_2:6879236-6879255 Msa0052580:CDS 30.0%
!! ATGTTGAACCCTTTTGTTTG+TGG - chr1_2:6878386-6878405 Msa0052580:CDS 35.0%
CATAGTTCATCATCATCTTC+TGG + chr1_2:6878422-6878441 None:intergenic 35.0%
!! TATCTTCTAGGGCTTGAATT+AGG + chr1_2:6878455-6878474 None:intergenic 35.0%
TGAATCATCAATTGTTGCCA+TGG - chr1_2:6878736-6878755 Msa0052580:CDS 35.0%
ATGAAATTCTCTTCTTGGGT+TGG + chr1_2:6878772-6878791 None:intergenic 35.0%
AACCCAAGAAGAGAATTTCA+TGG - chr1_2:6878771-6878790 Msa0052580:CDS 35.0%
!!! TTGGTTTTGTTGACTGTGTT+TGG - chr1_2:6878857-6878876 Msa0052580:CDS 35.0%
AGGTTTGAGTGAGAAGAAAA+TGG - chr1_2:6878982-6879001 Msa0052580:CDS 35.0%
TGGATGTAGTGAATAATGAC+GGG - chr1_2:6879002-6879021 Msa0052580:CDS 35.0%
AAGATGGAGTGAGAAGAAAA+TGG - chr1_2:6879135-6879154 Msa0052580:CDS 35.0%
!! ATGTTGAACCCTTTTGTTTG+TGG - chr1_2:6878386-6878405 Msa0052580:CDS 35.0%
CATAGTTCATCATCATCTTC+TGG + chr1_2:6878422-6878441 None:intergenic 35.0%
!! TATCTTCTAGGGCTTGAATT+AGG + chr1_2:6878455-6878474 None:intergenic 35.0%
TGAATCATCAATTGTTGCCA+TGG - chr1_2:6878736-6878755 Msa0052580:CDS 35.0%
ATGAAATTCTCTTCTTGGGT+TGG + chr1_2:6878772-6878791 None:intergenic 35.0%
AACCCAAGAAGAGAATTTCA+TGG - chr1_2:6878771-6878790 Msa0052580:CDS 35.0%
!!! TTGGTTTTGTTGACTGTGTT+TGG - chr1_2:6878857-6878876 Msa0052580:CDS 35.0%
AGGTTTGAGTGAGAAGAAAA+TGG - chr1_2:6878982-6879001 Msa0052580:CDS 35.0%
TGGATGTAGTGAATAATGAC+GGG - chr1_2:6879002-6879021 Msa0052580:CDS 35.0%
AAGATGGAGTGAGAAGAAAA+TGG - chr1_2:6879135-6879154 Msa0052580:CDS 35.0%
AATAACCCTTATTCCACACG+TGG - chr1_2:6878494-6878513 Msa0052580:CDS 40.0%
AAGCTTGAGTCCTAAGTCAA+CGG - chr1_2:6878697-6878716 Msa0052580:CDS 40.0%
!!! GACTTTTTCTCCGTTGACTT+AGG + chr1_2:6878710-6878729 None:intergenic 40.0%
GGATGTAGTGAATAATGACG+GGG - chr1_2:6879003-6879022 Msa0052580:CDS 40.0%
GGGTGGTGAAGAAAAATGAA+GGG - chr1_2:6879023-6879042 Msa0052580:CDS 40.0%
GGTGGTGAAGAAAAATGAAG+GGG - chr1_2:6879024-6879043 Msa0052580:CDS 40.0%
!! GGATTAGTTTCTCCAAGAAG+TGG - chr1_2:6879172-6879191 Msa0052580:CDS 40.0%
TTAGTTTCTCCAAGAAGTGG+TGG - chr1_2:6879175-6879194 Msa0052580:CDS 40.0%
TCAAAATCACCACCACTTCT+TGG + chr1_2:6879187-6879206 None:intergenic 40.0%
AATAACCCTTATTCCACACG+TGG - chr1_2:6878494-6878513 Msa0052580:CDS 40.0%
AAGCTTGAGTCCTAAGTCAA+CGG - chr1_2:6878697-6878716 Msa0052580:CDS 40.0%
!!! GACTTTTTCTCCGTTGACTT+AGG + chr1_2:6878710-6878729 None:intergenic 40.0%
GGATGTAGTGAATAATGACG+GGG - chr1_2:6879003-6879022 Msa0052580:CDS 40.0%
GGGTGGTGAAGAAAAATGAA+GGG - chr1_2:6879023-6879042 Msa0052580:CDS 40.0%
GGTGGTGAAGAAAAATGAAG+GGG - chr1_2:6879024-6879043 Msa0052580:CDS 40.0%
!! GGATTAGTTTCTCCAAGAAG+TGG - chr1_2:6879172-6879191 Msa0052580:CDS 40.0%
TTAGTTTCTCCAAGAAGTGG+TGG - chr1_2:6879175-6879194 Msa0052580:CDS 40.0%
TCAAAATCACCACCACTTCT+TGG + chr1_2:6879187-6879206 None:intergenic 40.0%
AAATTTATCCAGGCCACGTG+TGG + chr1_2:6878510-6878529 None:intergenic 45.0%
GACTTAGGACTCAAGCTTCT+TGG + chr1_2:6878695-6878714 None:intergenic 45.0%
!! GGGTTGGTTTCTTTCTTCCA+TGG + chr1_2:6878756-6878775 None:intergenic 45.0%
! TGTAGTGAATAATGACGGGG+TGG - chr1_2:6879006-6879025 Msa0052580:CDS 45.0%
GGGGTGGTGAAGAAAAATGA+AGG - chr1_2:6879022-6879041 Msa0052580:CDS 45.0%
GGTGAAGAAAAATGAAGGGG+TGG - chr1_2:6879027-6879046 Msa0052580:CDS 45.0%
AAATTTATCCAGGCCACGTG+TGG + chr1_2:6878510-6878529 None:intergenic 45.0%
GACTTAGGACTCAAGCTTCT+TGG + chr1_2:6878695-6878714 None:intergenic 45.0%
!! GGGTTGGTTTCTTTCTTCCA+TGG + chr1_2:6878756-6878775 None:intergenic 45.0%
! TGTAGTGAATAATGACGGGG+TGG - chr1_2:6879006-6879025 Msa0052580:CDS 45.0%
GGGGTGGTGAAGAAAAATGA+AGG - chr1_2:6879022-6879041 Msa0052580:CDS 45.0%
GGTGAAGAAAAATGAAGGGG+TGG - chr1_2:6879027-6879046 Msa0052580:CDS 45.0%
CCAGGCCACGTGTGGAATAA+GGG + chr1_2:6878502-6878521 None:intergenic 55.0%
TCCAGGCCACGTGTGGAATA+AGG + chr1_2:6878503-6878522 None:intergenic 55.0%
CCAGGCCACGTGTGGAATAA+GGG + chr1_2:6878502-6878521 None:intergenic 55.0%
TCCAGGCCACGTGTGGAATA+AGG + chr1_2:6878503-6878522 None:intergenic 55.0%
CCCTTATTCCACACGTGGCC+TGG - chr1_2:6878499-6878518 Msa0052580:CDS 60.0%
CCCTTATTCCACACGTGGCC+TGG - chr1_2:6878499-6878518 Msa0052580:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 6878386 6879261 6878386 ID=Msa0052580;Name=Msa0052580
chr1_2 mRNA 6878386 6879261 6878386 ID=Msa0052580-mRNA-1;Parent=Msa0052580;Name=Msa0052580-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|291
chr1_2 exon 6878386 6879261 6878386 ID=Msa0052580-mRNA-1:exon:2288;Parent=Msa0052580-mRNA-1
chr1_2 CDS 6878386 6879261 6878386 ID=Msa0052580-mRNA-1:cds;Parent=Msa0052580-mRNA-1
Gene Sequence

>Msa0052580

ATGTTGAACCCTTTTGTTTGTGGAACATTCAATCCAGAAGATGATGATGAACTATGTCGTGCAAATCCTAATTCAAGCCCTAGAAGATACAAAAGAAAAGATAGCAAAAATAACCCTTATTCCACACGTGGCCTGGATAAATTTTCAGCACTTTTATCTGATTTAAATGATAAAAGGCAGAAAATTTATTCACAAATGAACCCTCATGATATTTCTTTTGTTCGTTTTGTTCATTCAAATAAAGATGATTTTGTCCCTATTGTTGTTAAGGTGAAAAACAAGAGCCAAGAGTTAAAAGTAGTGAAACCAAGAAGCTTGAGTCCTAAGTCAACGGAGAAAAAGTCTTCTGCTGAATCATCAATTGTTGCCATGGAAGAAAGAAACCAACCCAAGAAGAGAATTTCATGGAATATGAAGAATATTGAGATGGAGAAACCATTGTTCTATTTGCCATTTGTTGTGATAATGATTTTGGTTTTGTTGACTGTGTTTGGAAGAACAGCTGCAATAATTTTCACTTGTATTCTGTGGTATGTTATTCCAACTTTGAAGGATATTAAATCAAGAAATGAAATGAAGAAAAGAGATTATGTTAGAGGTTTGAGTGAGAAGAAAATGGATGTAGTGAATAATGACGGGGTGGTGAAGAAAAATGAAGGGGTGGTGAAGAAAAAAGATTATGTTAGAGGGTTTAGTGAAAAAAAGATGGTGGTTAATGAAGGGATTAACAAAAAGAAAGATTATGTTAGAAGATGGAGTGAGAAGAAAATGGTTACTACTAATGATGGATTAGTTTCTCCAAGAAGTGGTGGTGATTTTGATGATGTTTCCAAGAACAAGATTCAAGCAAAACATTCACACAAGAAAAGTTGGTGA

Protein sequence

>Msa0052580

MLNPFVCGTFNPEDDDELCRANPNSSPRRYKRKDSKNNPYSTRGLDKFSALLSDLNDKRQKIYSQMNPHDISFVRFVHSNKDDFVPIVVKVKNKSQELKVVKPRSLSPKSTEKKSSAESSIVAMEERNQPKKRISWNMKNIEMEKPLFYLPFVVIMILVLLTVFGRTAAIIFTCILWYVIPTLKDIKSRNEMKKRDYVRGLSEKKMDVVNNDGVVKKNEGVVKKKDYVRGFSEKKMVVNEGINKKKDYVRRWSEKKMVTTNDGLVSPRSGGDFDDVSKNKIQAKHSHKKSW*