AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0590810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0590810 MtrunA17_Chr4g0008521 95.979 572 23 0 1 572 1 572 0.0 1125
Msa0590810 MtrunA17_Chr7g0240971 62.261 575 209 5 1 572 1 570 0.0 715
Msa0590810 MtrunA17_Chr7g0240991 57.544 570 228 9 10 572 9 571 0.0 684
Msa0590810 MtrunA17_Chr5g0404361 45.760 566 288 5 8 572 64 611 0.0 536
Msa0590810 MtrunA17_Chr5g0437681 47.803 569 274 9 14 572 12 567 0.0 533
Msa0590810 MtrunA17_Chr5g0431581 47.331 562 280 8 14 572 12 560 0.0 525
Msa0590810 MtrunA17_Chr3g0114211 48.155 569 274 9 12 572 15 570 0.0 524
Msa0590810 MtrunA17_Chr5g0404341 44.326 564 295 6 12 572 19 566 7.15e-176 509
Msa0590810 MtrunA17_Chr5g0429511 44.565 552 291 4 21 572 22 558 2.77e-173 502
Msa0590810 MtrunA17_Chr4g0005921 45.263 570 293 5 8 572 2 557 1.31e-172 500
Msa0590810 MtrunA17_Chr3g0104061 44.102 551 292 4 22 572 22 556 4.33e-172 499
Msa0590810 MtrunA17_Chr7g0238981 45.098 561 278 7 27 572 48 593 7.90e-172 499
Msa0590810 MtrunA17_Chr7g0238941 45.234 577 295 6 4 572 15 578 8.13e-171 496
Msa0590810 MtrunA17_Chr7g0237491 45.299 585 289 10 7 572 8 580 3.40e-168 490
Msa0590810 MtrunA17_Chr5g0436461 43.706 572 293 7 4 572 9 554 9.26e-166 483
Msa0590810 MtrunA17_Chr7g0237441 44.696 575 286 9 15 572 21 580 3.58e-165 482
Msa0590810 MtrunA17_Chr7g0238351 44.779 565 289 7 21 572 27 581 1.65e-164 481
Msa0590810 MtrunA17_Chr4g0031901 45.191 551 281 8 13 555 31 568 1.69e-164 481
Msa0590810 MtrunA17_Chr2g0278811 40.464 603 310 9 5 572 19 607 1.67e-160 471
Msa0590810 MtrunA17_Chr8g0346581 40.876 548 309 8 28 572 36 571 8.52e-150 442
Msa0590810 MtrunA17_Chr3g0133861 41.228 342 182 11 228 562 1 330 5.89e-75 241
Msa0590810 MtrunA17_Chr3g0133871 47.959 196 101 1 12 206 17 212 2.36e-61 202
Msa0590810 MtrunA17_Chr8g0382691 30.404 569 312 20 21 551 29 551 1.28e-60 211
Msa0590810 MtrunA17_Chr3g0118321 28.720 578 351 17 1 550 1 545 1.07e-54 194
Msa0590810 MtrunA17_Chr3g0118331 28.265 559 334 17 23 550 22 544 4.76e-48 176
Msa0590810 MtrunA17_Chr5g0436431 37.190 242 131 5 292 530 5 228 2.71e-44 157
Msa0590810 MtrunA17_Chr4g0056511 26.620 571 371 13 14 572 15 549 1.52e-40 155
Msa0590810 MtrunA17_Chr3g0079321 25.473 581 394 11 1 572 1 551 1.50e-39 152
Msa0590810 MtrunA17_Chr4g0038671 25.599 543 338 18 11 538 7 498 2.41e-31 128
Msa0590810 MtrunA17_Chr3g0143671 25.235 531 340 14 49 572 53 533 5.91e-31 127
Msa0590810 MtrunA17_Chr8g0374261 25.486 514 319 16 29 532 32 491 1.85e-30 125
Msa0590810 MtrunA17_Chr8g0374271 25.532 517 315 15 29 532 34 493 2.01e-30 125
Msa0590810 MtrunA17_Chr3g0121161 24.638 552 370 16 8 554 48 558 3.18e-29 122
Msa0590810 MtrunA17_Chr5g0402831 25.725 552 349 16 28 572 33 530 8.37e-29 120
Msa0590810 MtrunA17_Chr3g0121171 24.319 551 373 15 8 554 1158 1668 8.57e-29 122
Msa0590810 MtrunA17_Chr3g0121171 24.457 552 371 16 8 554 583 1093 1.08e-28 122
Msa0590810 MtrunA17_Chr3g0121171 24.304 539 364 15 20 554 20 518 1.46e-27 118
Msa0590810 MtrunA17_Chr3g0121171 23.732 552 366 17 8 554 1733 2234 3.25e-23 105
Msa0590810 MtrunA17_Chr4g0024861 24.457 552 360 17 28 572 29 530 9.35e-29 120
Msa0590810 MtrunA17_Chr2g0322441 25.000 552 357 17 28 572 32 533 1.23e-28 120
Msa0590810 MtrunA17_Chr3g0121151 24.549 554 368 17 8 554 48 558 1.26e-28 120
Msa0590810 MtrunA17_Chr7g0234061 25.189 528 341 18 19 532 22 509 5.43e-27 115
Msa0590810 MtrunA17_Chr1g0157341 23.585 530 350 14 49 572 52 532 6.19e-27 115
Msa0590810 MtrunA17_Chr4g0043261 23.188 552 370 15 8 552 7 511 7.96e-27 114
Msa0590810 MtrunA17_Chr4g0043251 24.612 516 341 16 43 552 44 517 5.35e-24 105
Msa0590810 MtrunA17_Chr3g0118341 36.000 150 92 4 28 175 28 175 5.62e-22 94.4
Msa0590810 MtrunA17_Chr4g0075071 25.051 495 306 13 49 532 54 494 1.25e-21 98.6
Msa0590810 MtrunA17_Chr3g0118351 28.135 327 206 11 238 550 5 316 6.41e-20 91.7
Msa0590810 MtrunA17_Chr4g0024641 24.000 450 286 17 92 535 17 416 4.85e-15 78.6
Msa0590810 MtrunA17_Chr1g0197311 28.641 206 111 6 44 217 42 243 6.66e-13 68.9
Msa0590810 MtrunA17_Chr3g0118931 39.130 69 42 0 482 550 10 78 3.64e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0590810 AT3G09220.1 58.409 553 215 7 8 555 8 550 0.0 671
Msa0590810 AT5G01050.1 54.690 565 242 5 12 572 14 568 0.0 648
Msa0590810 AT5G01040.1 53.217 575 254 8 1 572 4 566 0.0 638
Msa0590810 AT3G09220.2 58.225 462 178 6 99 555 2 453 0.0 554
Msa0590810 AT5G05390.1 47.719 570 284 6 6 572 7 565 0.0 547
Msa0590810 AT2G40370.1 46.967 577 278 9 13 572 15 580 0.0 527
Msa0590810 AT5G03260.1 46.491 570 287 5 4 572 5 557 2.57e-180 520
Msa0590810 AT2G30210.1 46.367 578 285 8 6 572 7 570 1.52e-179 519
Msa0590810 AT5G58910.2 46.561 567 283 6 12 572 15 567 1.83e-176 511
Msa0590810 AT5G58910.3 46.737 567 281 7 12 572 15 566 1.66e-175 508
Msa0590810 AT2G38080.1 44.406 572 300 6 1 572 5 558 4.30e-175 507
Msa0590810 AT5G07130.1 46.875 576 280 11 11 572 6 569 5.11e-174 504
Msa0590810 AT2G29130.1 45.629 572 295 6 8 572 11 573 6.87e-173 502
Msa0590810 AT5G01190.1 45.374 562 292 4 12 572 11 558 1.31e-170 496
Msa0590810 AT5G58910.1 46.753 539 261 6 39 572 6 523 5.41e-169 490
Msa0590810 AT5G60020.1 44.097 576 296 6 13 572 12 577 2.39e-167 488
Msa0590810 AT5G60020.3 45.290 552 276 6 37 572 4 545 1.02e-166 485
Msa0590810 AT5G09360.1 42.984 563 300 9 14 572 24 569 7.71e-166 484
Msa0590810 AT5G01190.2 44.840 562 281 5 12 572 11 544 1.47e-164 479
Msa0590810 AT1G18140.1 43.137 561 289 9 30 572 33 581 2.47e-156 460
Msa0590810 AT2G46570.1 42.806 556 297 9 23 572 29 569 1.62e-153 452
Msa0590810 AT5G60020.2 43.446 534 276 6 13 530 12 535 3.38e-148 438
Msa0590810 AT5G07130.2 47.262 493 237 10 91 572 4 484 8.82e-147 432
Msa0590810 AT5G07130.3 47.262 493 237 10 91 572 4 484 8.82e-147 432
Msa0590810 AT5G48100.1 40.429 559 300 12 12 555 6 546 5.28e-143 425
Msa0590810 AT2G46570.2 42.685 499 265 9 80 572 1 484 3.67e-133 397
Msa0590810 AT5G21100.1 29.217 575 343 16 21 567 19 557 4.77e-62 214
Msa0590810 AT4G39830.1 29.088 581 328 19 13 551 23 561 2.39e-60 210
Msa0590810 AT5G21105.1 27.768 569 353 13 10 550 20 558 1.04e-57 203
Msa0590810 AT5G21105.3 27.140 549 342 13 10 530 20 538 9.59e-49 178
Msa0590810 AT4G39830.2 28.543 508 280 18 85 551 1 466 3.60e-45 167
Msa0590810 AT4G39830.3 28.628 503 276 18 90 551 4 464 1.26e-44 165
Msa0590810 AT4G39830.4 28.008 507 271 17 85 551 1 453 9.08e-42 157
Msa0590810 AT4G25240.2 27.051 573 369 16 12 572 15 550 7.58e-41 156
Msa0590810 AT4G25240.1 27.051 573 369 16 12 572 15 550 7.58e-41 156
Msa0590810 AT5G51480.1 25.569 571 377 14 14 572 16 550 3.38e-40 154
Msa0590810 AT4G12420.1 24.764 529 358 14 12 532 11 507 5.85e-35 139
Msa0590810 AT4G12420.2 24.764 529 358 14 12 532 11 507 5.85e-35 139
Msa0590810 AT1G41830.1 25.391 575 358 17 14 572 12 531 1.21e-33 135
Msa0590810 AT1G76160.1 25.130 577 371 18 7 572 4 530 3.60e-32 130
Msa0590810 AT1G21860.1 25.376 532 338 17 14 538 17 496 3.60e-32 130
Msa0590810 AT4G37160.1 24.525 579 371 15 4 572 12 534 6.63e-32 130
Msa0590810 AT5G48450.2 23.836 537 367 15 14 547 17 514 1.12e-31 130
Msa0590810 AT5G48450.1 23.836 537 367 15 14 547 17 514 2.03e-31 129
Msa0590810 AT1G75790.1 24.564 574 371 19 14 572 15 541 1.16e-30 126
Msa0590810 AT1G21850.1 25.151 497 313 16 49 538 52 496 4.66e-30 124
Msa0590810 AT2G23630.2 24.954 545 363 13 29 572 38 537 1.44e-29 123
Msa0590810 AT2G23630.1 24.954 545 363 13 29 572 33 532 1.54e-29 123
Msa0590810 AT5G66920.1 23.835 558 354 14 28 572 39 538 6.36e-29 121
Msa0590810 AT1G55560.1 30.612 294 194 5 28 320 28 312 1.88e-28 120
Msa0590810 AT3G13400.2 24.366 513 336 13 28 532 29 497 1.97e-27 117
Msa0590810 AT3G13400.1 24.366 513 336 13 28 532 29 497 1.97e-27 117
Msa0590810 AT5G21105.2 25.656 343 226 5 211 550 51 367 6.78e-27 113
Msa0590810 AT4G22010.1 24.767 537 336 17 49 572 49 530 1.08e-26 114
Msa0590810 AT4G37160.2 28.349 321 212 5 4 323 12 315 2.23e-26 112
Msa0590810 AT1G55570.1 24.105 531 355 12 12 532 8 500 6.90e-26 112
Msa0590810 AT3G13390.1 25.245 511 341 15 28 535 30 502 2.91e-25 110
Msa0590810 AT4G28090.1 23.600 500 322 16 43 532 45 494 2.36e-21 98.2
Msa0590810 AT4G38420.2 28.405 257 160 7 37 285 41 281 4.29e-18 87.8
Msa0590810 AT4G38420.3 28.405 257 160 7 37 285 41 281 8.10e-18 87.0
Msa0590810 AT4G38420.1 28.405 257 160 7 37 285 41 281 1.53e-17 86.3

Find 146 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATAAAATGTGTCATATT+TGG 0.185163 4_2:-14527989 None:intergenic
TTAAACATTATGCAGGTATT+TGG 0.193690 4_2:+14533123 Msa0590810:intron
GGGCATTGGGTTACATATTC+TGG 0.208610 4_2:-14530243 None:intergenic
AATTCAAATGGTGGTTGATT+TGG 0.228784 4_2:-14532701 None:intergenic
GAAAGAATAGGCAAACATTT+TGG 0.246426 4_2:-14533393 None:intergenic
AATTAACTCAAGAATTTATA+AGG 0.257962 4_2:-14533289 None:intergenic
TCATGGTTATCAGGCATAAT+TGG 0.277334 4_2:-14531875 None:intergenic
TGGGTTTAACTTTCATGTTT+TGG 0.277831 4_2:+14532886 Msa0590810:CDS
TCAATTTGATAAATGTTTAC+AGG 0.279128 4_2:-14533440 None:intergenic
GAACACATGTTCATAACTTT+TGG 0.285867 4_2:+14532509 Msa0590810:CDS
GTCTTCACCTTAGTTGATTT+AGG 0.296358 4_2:-14532782 None:intergenic
TCAACAATTAATGGCTTATC+TGG 0.303948 4_2:+14530889 Msa0590810:CDS
ACCTACCAATTTAACCTAAC+TGG 0.312872 4_2:+14530288 Msa0590810:CDS
CTTGTCCAGTTAGGTTAAAT+TGG 0.320988 4_2:-14530293 None:intergenic
TCAAATGCATCAACAATTAA+TGG 0.327150 4_2:+14530880 Msa0590810:CDS
GGGACTTCTTGATAAACCTT+TGG 0.333644 4_2:-14530414 None:intergenic
TCAGGCATAATTGGCATTAT+AGG 0.339643 4_2:-14531866 None:intergenic
CCTACAATCTTACTCTTCAT+TGG 0.348502 4_2:+14528936 Msa0590810:CDS
TCCTCTAGTGGTTATGTTAG+TGG 0.350570 4_2:-14531807 None:intergenic
AATCATCCTATTCATATTCA+TGG 0.354176 4_2:+14532866 Msa0590810:CDS
CACCAGATGGTATTGGGCAT+TGG 0.364496 4_2:-14530257 None:intergenic
AGCTGCCACCAGATGGTATT+GGG 0.364532 4_2:-14530263 None:intergenic
CTACAAAATACAGCTATTCT+TGG 0.369525 4_2:+14532836 Msa0590810:CDS
TAATTATACTGACCCTTCAT+TGG 0.373770 4_2:+14532724 Msa0590810:CDS
TTGGCTTGTACATTGTCATT+TGG 0.376918 4_2:+14533142 Msa0590810:CDS
GCAAATGCAAGTGGCTTAGA+AGG 0.379879 4_2:-14532758 None:intergenic
CCCTCTCGAGCATTAATTGT+TGG 0.386584 4_2:-14528878 None:intergenic
AAAACAATACCAAGCAATAT+GGG 0.389972 4_2:-14530435 None:intergenic
GTTGGTGTATCATGGTTATC+AGG 0.392956 4_2:-14531884 None:intergenic
AATTGAAGACTTACGATCTT+GGG 0.401325 4_2:-14530929 None:intergenic
GATGTTGTGGTGCTTGCGCC+TGG 0.402477 4_2:+14531695 Msa0590810:CDS
ACCAGATGGTATTGGGCATT+GGG 0.403258 4_2:-14530256 None:intergenic
TCATCTACTTTACGTGGAAC+TGG 0.407962 4_2:-14532488 None:intergenic
TACAAAATACAGCTATTCTT+GGG 0.408559 4_2:+14532837 Msa0590810:CDS
TAATTGAAGACTTACGATCT+TGG 0.410547 4_2:-14530930 None:intergenic
TAGCTACAACTGTGAAACTA+TGG 0.411298 4_2:-14531640 None:intergenic
TCCTCCCACTGGCACAGAAA+TGG 0.414706 4_2:-14532984 None:intergenic
TCCTGGTAAGGTTCCATTGA+CGG 0.426598 4_2:-14528855 None:intergenic
GTGTACCCTCTTGTCCAGTT+AGG 0.427115 4_2:-14530302 None:intergenic
TTTCTGTGCCAGTGGGAGGA+TGG 0.429026 4_2:+14532987 Msa0590810:CDS
TTAAACCCATGAATATGAAT+AGG 0.429980 4_2:-14532872 None:intergenic
GGACTTCTTGATAAACCTTT+GGG 0.434862 4_2:-14530413 None:intergenic
GTTGAGAATGGGCCTACTCT+TGG 0.437907 4_2:+14533203 Msa0590810:CDS
CTAGTCGTGGCTTAATGATA+AGG 0.437923 4_2:-14530374 None:intergenic
TCAAGAGAACGTCAACGGTT+TGG 0.441613 4_2:-14531718 None:intergenic
TTCCTACGTGCTACTGTCCA+TGG 0.442379 4_2:+14530348 Msa0590810:CDS
TTTGCTCTTCCTAGGGGTAG+AGG 0.443734 4_2:+14532620 Msa0590810:CDS
CCAATGAAGAGTAAGATTGT+AGG 0.448345 4_2:-14528936 None:intergenic
GAAGCTAATAGAAGAGCAAA+AGG 0.450795 4_2:-14528025 None:intergenic
TTCTGTGCCAGTGGGAGGAT+GGG 0.454325 4_2:+14532988 Msa0590810:CDS
CACTATGATGTTGAAAGAAT+AGG 0.456772 4_2:-14533405 None:intergenic
CAGGTTATCACTGCCGTCAA+TGG 0.458437 4_2:+14528842 Msa0590810:CDS
ATATATACAGGTTTAACAAC+TGG 0.461888 4_2:+14532452 Msa0590810:intron
TTTAACAACTGGTCCACATT+GGG 0.462574 4_2:+14532463 Msa0590810:CDS
ATTGATAATGCGTAGTAAGT+AGG 0.465795 4_2:-14531579 None:intergenic
AGAGGAGTTGTTGTTTATGA+TGG 0.466459 4_2:+14531824 Msa0590810:CDS
TTATATCATACCTGTAACAT+TGG 0.468461 4_2:-14531924 None:intergenic
ATTTGCACCTAAATCAACTA+AGG 0.469161 4_2:+14532775 Msa0590810:CDS
TAGCTGCCACCAGATGGTAT+TGG 0.469636 4_2:-14530264 None:intergenic
TCAGTATAATTAAATTCAAA+TGG 0.470555 4_2:-14532713 None:intergenic
CTTGATAAACCTTTGGGAAG+GGG 0.471972 4_2:-14530407 None:intergenic
GCCGTCAATGGAACCTTACC+AGG 0.472419 4_2:+14528854 Msa0590810:CDS
AGTTGTTGATTCTTATTACA+TGG 0.475969 4_2:+14531751 Msa0590810:CDS
GGATTAACAAAGTTAAACTT+TGG 0.476313 4_2:-14532947 None:intergenic
GAAATGGTGTTACGCATTTG+AGG 0.480620 4_2:-14532968 None:intergenic
TGAGAGAAATGCAACAGATA+CGG 0.485761 4_2:+14530840 Msa0590810:CDS
ATCATCCTATTCATATTCAT+GGG 0.488322 4_2:+14532867 Msa0590810:CDS
ATTAATTGTTGGTCCTGGTA+AGG 0.491356 4_2:-14528867 None:intergenic
CGAGCATTAATTGTTGGTCC+TGG 0.493195 4_2:-14528872 None:intergenic
GAGAGAAATGCAACAGATAC+GGG 0.494209 4_2:+14530841 Msa0590810:CDS
TCAGCGATTGTGCCGTCCAC+AGG 0.494835 4_2:+14528823 Msa0590810:CDS
GGGTAGAGGTTTCTCAATGT+TGG 0.495591 4_2:+14532634 Msa0590810:CDS
CAATTTGATAAATGTTTACA+GGG 0.497563 4_2:-14533439 None:intergenic
GGAAGATTAGCTGGTGGAGG+AGG 0.498166 4_2:-14533236 None:intergenic
CAAGAAGTCCCCATATTGCT+TGG 0.500129 4_2:+14530426 Msa0590810:CDS
AGAACGTCAACGGTTTGGCC+AGG 0.501113 4_2:-14531713 None:intergenic
ATTTGGAAACACACTTGCCA+TGG 0.502328 4_2:+14533159 Msa0590810:CDS
TAGTTTCACAGTTGTAGCTA+TGG 0.504059 4_2:+14531643 Msa0590810:CDS
TGTGAGCTGTTGGTGTATCA+TGG 0.504650 4_2:-14531892 None:intergenic
TTTGGAAACACACTTGCCAT+GGG 0.506002 4_2:+14533160 Msa0590810:CDS
GGATCAACATTAGCCAATGA+AGG 0.506492 4_2:-14532737 None:intergenic
ACAACACAACGACCTGGTCC+TGG 0.509215 4_2:-14532557 None:intergenic
ATTTGCTTCTGCTGCTATCG+TGG 0.515893 4_2:+14528051 Msa0590810:CDS
CAACACTTAGGAAGATTAGC+TGG 0.518544 4_2:-14533245 None:intergenic
TTCTTGATAAACCTTTGGGA+AGG 0.519043 4_2:-14530409 None:intergenic
AAAAGATTCATTGTTCATGT+TGG 0.521543 4_2:-14532600 None:intergenic
CAATGAAGAGTAAGATTGTA+GGG 0.523403 4_2:-14528935 None:intergenic
AGATTTCAAGCAAATAATCC+AGG 0.524094 4_2:+14533019 Msa0590810:CDS
ATTAAATTGCAGGGGAGTGG+TGG 0.524387 4_2:+14530797 Msa0590810:intron
ACCCAATGCCCAATACCATC+TGG 0.526254 4_2:+14530255 Msa0590810:CDS
TAGTCGTGGCTTAATGATAA+GGG 0.527616 4_2:-14530373 None:intergenic
AAACTTTGGTTCATCTCTAG+TGG 0.533014 4_2:-14532933 None:intergenic
CTTATCATTAAGCCACGACT+AGG 0.535485 4_2:+14530375 Msa0590810:CDS
TCTTGATAAACCTTTGGGAA+GGG 0.536055 4_2:-14530408 None:intergenic
ACCAACAATTAATGCTCGAG+AGG 0.542459 4_2:+14528877 Msa0590810:CDS
CCATACCGTTCTTCAAGTGT+TGG 0.544154 4_2:+14531782 Msa0590810:CDS
ATAATTAAATTGCAGGGGAG+TGG 0.546443 4_2:+14530794 Msa0590810:intron
TCCAGTTAGGTTAAATTGGT+AGG 0.550019 4_2:-14530289 None:intergenic
GTTTAACAACTGGTCCACAT+TGG 0.554162 4_2:+14532462 Msa0590810:CDS
GTTGATGCATTTGACTCCGC+TGG 0.558250 4_2:-14530871 None:intergenic
CTAGAGCAATGCATCAATCC+AGG 0.561417 4_2:+14532539 Msa0590810:CDS
GATGCATTTGACTCCGCTGG+TGG 0.562919 4_2:-14530868 None:intergenic
GACAAGAGGGTACACTTTGG+TGG 0.569591 4_2:+14530310 Msa0590810:CDS
ATTTAACCTAACTGGACAAG+AGG 0.571063 4_2:+14530296 Msa0590810:CDS
CAATGCATCAATCCAGGACC+AGG 0.571950 4_2:+14532545 Msa0590810:CDS
AAATAATGTACTCAACACTT+AGG 0.572543 4_2:-14533257 None:intergenic
CGTGGAACTGGAACCCAATG+TGG 0.578205 4_2:-14532476 None:intergenic
GTATAATTAAATTCAAATGG+TGG 0.578940 4_2:-14532710 None:intergenic
ACCATTTCTGTGCCAGTGGG+AGG 0.579775 4_2:+14532983 Msa0590810:CDS
TTAGGAAGATTAGCTGGTGG+AGG 0.587146 4_2:-14533239 None:intergenic
GATTTAGGTGCAAATGCAAG+TGG 0.589023 4_2:-14532767 None:intergenic
ATAACAGCCCATCCTCCCAC+TGG 0.590871 4_2:-14532995 None:intergenic
TACAAGCTCAAAGTGAAGCA+AGG 0.595483 4_2:+14531551 Msa0590810:CDS
CTGGACAAGAGGGTACACTT+TGG 0.595930 4_2:+14530307 Msa0590810:CDS
TGTAATAAGAATCAACAACT+TGG 0.597362 4_2:-14531748 None:intergenic
ATGTGTTCATCTACTTTACG+TGG 0.597501 4_2:-14532494 None:intergenic
GTGTAAAACTTGTGAGCTGT+TGG 0.601475 4_2:-14531902 None:intergenic
ATGCATTTGACTCCGCTGGT+GGG 0.601923 4_2:-14530867 None:intergenic
TAACACCATTTCTGTGCCAG+TGG 0.602566 4_2:+14532979 Msa0590810:CDS
ATAAACAACAACTCCTCTAG+TGG 0.606065 4_2:-14531819 None:intergenic
TAGGTATAGCTGCCACCAGA+TGG 0.606138 4_2:-14530270 None:intergenic
GGCACTGTTGTTCCAAGAGT+AGG 0.606785 4_2:-14533215 None:intergenic
CCAACACTTGAAGAACGGTA+TGG 0.608437 4_2:-14531782 None:intergenic
ATTGAGAAACCTCTACCCCT+AGG 0.617029 4_2:-14532629 None:intergenic
TGGTTCCAACACTTGAAGAA+CGG 0.619947 4_2:-14531787 None:intergenic
TGGAAACACACTTGCCATGG+GGG 0.620681 4_2:+14533162 Msa0590810:CDS
ACCACTAACATAACCACTAG+AGG 0.625523 4_2:+14531806 Msa0590810:CDS
TTTAACCTAACTGGACAAGA+GGG 0.625977 4_2:+14530297 Msa0590810:CDS
TCGCTCATACCCCTTCCCAA+AGG 0.634666 4_2:+14530398 Msa0590810:CDS
ACACTATAACACTGATGTTG+TGG 0.639226 4_2:+14531682 Msa0590810:CDS
ACAAAATACAGCTATTCTTG+GGG 0.644652 4_2:+14532838 Msa0590810:CDS
AAACAATACCAAGCAATATG+GGG 0.644866 4_2:-14530434 None:intergenic
GATCAACATTAGCCAATGAA+GGG 0.645575 4_2:-14532736 None:intergenic
CGGCAGTGATAACCTGTGGA+CGG 0.647467 4_2:-14528835 None:intergenic
TTGGAAACACACTTGCCATG+GGG 0.647612 4_2:+14533161 Msa0590810:CDS
AACACCATTTCTGTGCCAGT+GGG 0.650163 4_2:+14532980 Msa0590810:CDS
GGGTATGAGCGACCTAGTCG+TGG 0.653043 4_2:-14530387 None:intergenic
CACTTAGGAAGATTAGCTGG+TGG 0.654885 4_2:-14533242 None:intergenic
TTGACGGCAGTGATAACCTG+TGG 0.667579 4_2:-14528839 None:intergenic
CCAACAATTAATGCTCGAGA+GGG 0.669858 4_2:+14528878 Msa0590810:CDS
CAATGCCCAATACCATCTGG+TGG 0.674666 4_2:+14530258 Msa0590810:CDS
GCTTAATGATAAGGGCACCA+TGG 0.676516 4_2:-14530365 None:intergenic
TTTAGTACAACACAACGACC+TGG 0.677465 4_2:-14532563 None:intergenic
TATTCTGGACCATCTGACCA+AGG 0.704441 4_2:-14530228 None:intergenic
AGATACGGGTAACCCACCAG+CGG 0.756602 4_2:+14530855 Msa0590810:CDS
CACCATGGACAGTAGCACGT+AGG 0.762654 4_2:-14530350 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTTTTAAGTAAAATAA+AGG - chr4_2:14528170-14528189 None:intergenic 10.0%
!! ATATATAATAAGATAAACAA+AGG + chr4_2:14529930-14529949 Msa0590810:intron 10.0%
!!! CTATTTTTTTTTTATTGAAA+GGG - chr4_2:14530107-14530126 None:intergenic 10.0%
!!! ACTATTTTTTTTTTATTGAA+AGG - chr4_2:14530108-14530127 None:intergenic 10.0%
!!! TTTTAAATGTAATTCTAATT+TGG + chr4_2:14530160-14530179 Msa0590810:intron 10.0%
!! AAATATGAATTCTTATAATT+TGG - chr4_2:14530496-14530515 None:intergenic 10.0%
!!! AATTGTTTAGAATAATTTTT+TGG - chr4_2:14530760-14530779 None:intergenic 10.0%
!! TTCAATTAATTAACAAATTT+GGG + chr4_2:14530945-14530964 Msa0590810:intron 10.0%
!!! AATATGTATATCTTTTTTTT+AGG + chr4_2:14532391-14532410 Msa0590810:intron 10.0%
!! TAAATTTCCATATATTCTAA+AGG - chr4_2:14528131-14528150 None:intergenic 15.0%
!! TATAACTAGATTGAAATAAA+AGG + chr4_2:14528381-14528400 Msa0590810:intron 15.0%
!! TGTTACTAAACTTTATAATA+TGG + chr4_2:14528736-14528755 Msa0590810:intron 15.0%
!!! TTAATTTTATTTTGATATCC+AGG + chr4_2:14528784-14528803 Msa0590810:intron 15.0%
!!! TTCTCTAATATTTTTATTGA+GGG - chr4_2:14528981-14529000 None:intergenic 15.0%
!!! TTTCTCTAATATTTTTATTG+AGG - chr4_2:14528982-14529001 None:intergenic 15.0%
!!! TTACATGATCATTATTTTAA+CGG + chr4_2:14529141-14529160 Msa0590810:intron 15.0%
!!! ATTTTTTACATAGTTGTTTT+TGG - chr4_2:14529500-14529519 None:intergenic 15.0%
!!! CTTTAGAATATTCTTAAAAT+AGG - chr4_2:14529600-14529619 None:intergenic 15.0%
!!! AATCAATTTTATCATAAAAG+AGG + chr4_2:14529717-14529736 Msa0590810:intron 15.0%
!!! ATCAATTTTATCATAAAAGA+GGG + chr4_2:14529718-14529737 Msa0590810:intron 15.0%
!!! TTATTTTTCTCATATGTATA+GGG + chr4_2:14530129-14530148 Msa0590810:intron 15.0%
!! ATATATCATAATTAAATTGC+AGG + chr4_2:14530787-14530806 Msa0590810:intron 15.0%
!! TATATCATAATTAAATTGCA+GGG + chr4_2:14530788-14530807 Msa0590810:intron 15.0%
!! CTTCAATTAATTAACAAATT+TGG + chr4_2:14530944-14530963 Msa0590810:intron 15.0%
!! TCATATCTTAAAATATAGTT+TGG - chr4_2:14531397-14531416 None:intergenic 15.0%
!!! ATTTAATTTATATGCACTTT+CGG + chr4_2:14532017-14532036 Msa0590810:intron 15.0%
!! TTCAATGATCATAAATATAT+TGG + chr4_2:14532206-14532225 Msa0590810:intron 15.0%
!!! GAAATTTTGTATATATATAC+AGG + chr4_2:14532440-14532459 Msa0590810:intron 15.0%
!! TCAGTATAATTAAATTCAAA+TGG - chr4_2:14532716-14532735 None:intergenic 15.0%
!! AATTAACTCAAGAATTTATA+AGG - chr4_2:14533292-14533311 None:intergenic 15.0%
!! AACATAAAATGTGTCATATT+TGG - chr4_2:14527992-14528011 None:intergenic 20.0%
!!! ATTTCTTCCTTTAGAATATA+TGG + chr4_2:14528121-14528140 Msa0590810:intron 20.0%
!! CTAGTTATAAAGATTTACTA+TGG - chr4_2:14528370-14528389 None:intergenic 20.0%
!! AAGAAAAGATTAAAACAAGT+TGG - chr4_2:14528612-14528631 None:intergenic 20.0%
!!! TAATATTTTTATTGAGGGTA+AGG - chr4_2:14528976-14528995 None:intergenic 20.0%
!! GAAATAAATTAAACAAACCA+TGG + chr4_2:14528998-14529017 Msa0590810:intron 20.0%
!!! TTTCTTTTCCATTTTTTTAG+AGG + chr4_2:14529373-14529392 Msa0590810:intron 20.0%
!!! AATACTTTCAATCACTTTTT+AGG - chr4_2:14529775-14529794 None:intergenic 20.0%
!!! ATCAATTTTTTGACGAATTA+TGG - chr4_2:14529901-14529920 None:intergenic 20.0%
!! ATACTATTGTTAAAATGAGA+TGG - chr4_2:14530075-14530094 None:intergenic 20.0%
!!! GTTATTTTTCTCATATGTAT+AGG + chr4_2:14530128-14530147 Msa0590810:intron 20.0%
!!! GTTTTTTCACATTACATATT+TGG + chr4_2:14530453-14530472 Msa0590810:intron 20.0%
!!! TAAGAATTCATATTTTTCTG+TGG + chr4_2:14530501-14530520 Msa0590810:intron 20.0%
!! ATATCATAATTAAATTGCAG+GGG + chr4_2:14530789-14530808 Msa0590810:intron 20.0%
!!! TCTATTTTAGTAGATGAAAT+TGG + chr4_2:14530984-14531003 Msa0590810:intron 20.0%
!! TAAGAAATTTGGATAAAGAT+TGG - chr4_2:14531056-14531075 None:intergenic 20.0%
!! CATGTAAAAGTTAAGAAATT+TGG - chr4_2:14531067-14531086 None:intergenic 20.0%
!!! CTTTCTTTTACAATAAACTT+AGG + chr4_2:14531099-14531118 Msa0590810:intron 20.0%
!! AAATTCAATTATCTTTGGTA+AGG - chr4_2:14531330-14531349 None:intergenic 20.0%
!!! ATCTGAAATTCAATTATCTT+TGG - chr4_2:14531335-14531354 None:intergenic 20.0%
!! TTACAGGTATGATATAAATA+GGG + chr4_2:14531930-14531949 Msa0590810:intron 20.0%
!! TGTGTATATATAATCAACTT+TGG + chr4_2:14531987-14532006 Msa0590810:intron 20.0%
!! CACCTAATTATTATCAAATT+CGG + chr4_2:14532072-14532091 Msa0590810:intron 20.0%
!!! ATCCGAATTTGATAATAATT+AGG - chr4_2:14532077-14532096 None:intergenic 20.0%
!! TACTCTATAGTGAAAATATT+TGG + chr4_2:14532353-14532372 Msa0590810:intron 20.0%
!! GTATAATTAAATTCAAATGG+TGG - chr4_2:14532713-14532732 None:intergenic 20.0%
!!! ATTGACAAAAAGTGTAATAA+AGG + chr4_2:14533370-14533389 Msa0590810:three_prime_UTR 20.0%
!! CAATTTGATAAATGTTTACA+GGG - chr4_2:14533442-14533461 None:intergenic 20.0%
!! TCAATTTGATAAATGTTTAC+AGG - chr4_2:14533443-14533462 None:intergenic 20.0%
!! ATTTTATGTTTTCTCTAGCA+TGG + chr4_2:14528002-14528021 Msa0590810:CDS 25.0%
! TACTTTCAATGTATTGTTGA+TGG + chr4_2:14528078-14528097 Msa0590810:intron 25.0%
! ACTTTCAATGTATTGTTGAT+GGG + chr4_2:14528079-14528098 Msa0590810:intron 25.0%
! ATAAAGGAAGAAAGCTATAT+AGG - chr4_2:14528154-14528173 None:intergenic 25.0%
! ATGATCCACATGAAAAAAAT+AGG + chr4_2:14528263-14528282 Msa0590810:intron 25.0%
! TGATCCACATGAAAAAAATA+GGG + chr4_2:14528264-14528283 Msa0590810:intron 25.0%
!!! TTTTCCCTATTTTTTTCATG+TGG - chr4_2:14528271-14528290 None:intergenic 25.0%
!! CAATCTATTTTCTTTTGCAA+AGG - chr4_2:14528537-14528556 None:intergenic 25.0%
! TCTAAAAAAGCTCAAACTTA+AGG - chr4_2:14528695-14528714 None:intergenic 25.0%
! TAATTCTTGAATTCAAACCA+TGG - chr4_2:14529018-14529037 None:intergenic 25.0%
! AAATACAAGAAACGTTTAAC+AGG - chr4_2:14529063-14529082 None:intergenic 25.0%
! TTTAAAAACATATGAGACTC+CGG + chr4_2:14529793-14529812 Msa0590810:intron 25.0%
! GGTTACATTATAAAATATGC+AGG + chr4_2:14530181-14530200 Msa0590810:intron 25.0%
!!! TCATTCAATTTTTAACTCCT+TGG + chr4_2:14530211-14530230 Msa0590810:CDS 25.0%
! AAAACAATACCAAGCAATAT+GGG - chr4_2:14530438-14530457 None:intergenic 25.0%
! AAAAACAATACCAAGCAATA+TGG - chr4_2:14530439-14530458 None:intergenic 25.0%
! CTATCAAACGTCAATAAATA+TGG + chr4_2:14530666-14530685 Msa0590810:intron 25.0%
! AGAAATGAATTCAAATGTCA+TGG - chr4_2:14530706-14530725 None:intergenic 25.0%
! TCAAATGCATCAACAATTAA+TGG + chr4_2:14530880-14530899 Msa0590810:CDS 25.0%
!!! TCATGACTGTTTTAACATTT+TGG + chr4_2:14531506-14531525 Msa0590810:intron 25.0%
! TGTAATAAGAATCAACAACT+TGG - chr4_2:14531751-14531770 None:intergenic 25.0%
!!! AGTTGTTGATTCTTATTACA+TGG + chr4_2:14531751-14531770 Msa0590810:CDS 25.0%
! TTATATCATACCTGTAACAT+TGG - chr4_2:14531927-14531946 None:intergenic 25.0%
! GTTACAGGTATGATATAAAT+AGG + chr4_2:14531929-14531948 Msa0590810:intron 25.0%
! CATTTACTAATTGCAAGATT+CGG + chr4_2:14532103-14532122 Msa0590810:intron 25.0%
! ATATATACAGGTTTAACAAC+TGG + chr4_2:14532452-14532471 Msa0590810:intron 25.0%
!! ATGTTCATAACTTTTGGTAT+TGG + chr4_2:14532515-14532534 Msa0590810:CDS 25.0%
! AAAAGATTCATTGTTCATGT+TGG - chr4_2:14532603-14532622 None:intergenic 25.0%
!! GCATTTTACAACAATATTAG+TGG + chr4_2:14532659-14532678 Msa0590810:CDS 25.0%
! TACAAAATACAGCTATTCTT+GGG + chr4_2:14532837-14532856 Msa0590810:CDS 25.0%
! AATCATCCTATTCATATTCA+TGG + chr4_2:14532866-14532885 Msa0590810:CDS 25.0%
! ATCATCCTATTCATATTCAT+GGG + chr4_2:14532867-14532886 Msa0590810:CDS 25.0%
! TTAAACCCATGAATATGAAT+AGG - chr4_2:14532875-14532894 None:intergenic 25.0%
! GGATTAACAAAGTTAAACTT+TGG - chr4_2:14532950-14532969 None:intergenic 25.0%
! TTCTACTTTAAACATTATGC+AGG + chr4_2:14533116-14533135 Msa0590810:intron 25.0%
! TTAAACATTATGCAGGTATT+TGG + chr4_2:14533123-14533142 Msa0590810:intron 25.0%
! AAATAATGTACTCAACACTT+AGG - chr4_2:14533260-14533279 None:intergenic 25.0%
AGAAATGAGAGATGTAGAAA+AGG - chr4_2:14528107-14528126 None:intergenic 30.0%
TGTATAGTCCTTATGTAAGA+AGG + chr4_2:14528209-14528228 Msa0590810:intron 30.0%
AAAGCTCAAACTTAAGGTAA+TGG - chr4_2:14528689-14528708 None:intergenic 30.0%
CAATGAAGAGTAAGATTGTA+GGG - chr4_2:14528938-14528957 None:intergenic 30.0%
TCAACATTATACTACATGCA+TGG - chr4_2:14529105-14529124 None:intergenic 30.0%
! AGCAATATCTTCTTGTTTCT+AGG - chr4_2:14529314-14529333 None:intergenic 30.0%
! AGCAAGATCCTCTAAAAAAA+TGG - chr4_2:14529384-14529403 None:intergenic 30.0%
TTAAAAACATATGAGACTCC+GGG + chr4_2:14529794-14529813 Msa0590810:intron 30.0%
! GCTAAATTTTCTTTCTTTCC+CGG - chr4_2:14529815-14529834 None:intergenic 30.0%
CATTATAAAATATGCAGGCA+TGG + chr4_2:14530186-14530205 Msa0590810:intron 30.0%
AAACAATACCAAGCAATATG+GGG - chr4_2:14530437-14530456 None:intergenic 30.0%
TCAACAATTAATGGCTTATC+TGG + chr4_2:14530889-14530908 Msa0590810:CDS 30.0%
AATTGAAGACTTACGATCTT+GGG - chr4_2:14530932-14530951 None:intergenic 30.0%
TAATTGAAGACTTACGATCT+TGG - chr4_2:14530933-14530952 None:intergenic 30.0%
TCTCCACTTATTCACTTAAA+GGG - chr4_2:14531203-14531222 None:intergenic 30.0%
TTCTCCACTTATTCACTTAA+AGG - chr4_2:14531204-14531223 None:intergenic 30.0%
AAGTGAATAAGTGGAGAATA+AGG + chr4_2:14531206-14531225 Msa0590810:intron 30.0%
AGTGAATAAGTGGAGAATAA+GGG + chr4_2:14531207-14531226 Msa0590810:intron 30.0%
! TTTTACATTGCAGTATGAAG+AGG - chr4_2:14531244-14531263 None:intergenic 30.0%
TTCAATTATCTTTGGTAAGG+AGG - chr4_2:14531327-14531346 None:intergenic 30.0%
AAAACAGTCATGAGTCATAT+GGG - chr4_2:14531499-14531518 None:intergenic 30.0%
TAAAACAGTCATGAGTCATA+TGG - chr4_2:14531500-14531519 None:intergenic 30.0%
!! ATTGATAATGCGTAGTAAGT+AGG - chr4_2:14531582-14531601 None:intergenic 30.0%
!! CATTATAGGTGCTTTTGTTT+GGG - chr4_2:14531855-14531874 None:intergenic 30.0%
! AAGTTTTACACCAATGTTAC+AGG + chr4_2:14531914-14531933 Msa0590810:CDS 30.0%
TGATATAAATAGGGTTTCAC+TGG + chr4_2:14531939-14531958 Msa0590810:intron 30.0%
! CTTTCGGTGTAAATTAACTT+TGG + chr4_2:14532033-14532052 Msa0590810:intron 30.0%
! GAACACATGTTCATAACTTT+TGG + chr4_2:14532509-14532528 Msa0590810:CDS 30.0%
AATTCAAATGGTGGTTGATT+TGG - chr4_2:14532704-14532723 None:intergenic 30.0%
TAATTATACTGACCCTTCAT+TGG + chr4_2:14532724-14532743 Msa0590810:CDS 30.0%
ATTTGCACCTAAATCAACTA+AGG + chr4_2:14532775-14532794 Msa0590810:CDS 30.0%
CTACAAAATACAGCTATTCT+TGG + chr4_2:14532836-14532855 Msa0590810:CDS 30.0%
ACAAAATACAGCTATTCTTG+GGG + chr4_2:14532838-14532857 Msa0590810:CDS 30.0%
!! TGGGTTTAACTTTCATGTTT+TGG + chr4_2:14532886-14532905 Msa0590810:CDS 30.0%
AGATTTCAAGCAAATAATCC+AGG + chr4_2:14533019-14533038 Msa0590810:CDS 30.0%
ACATGTAAATAAAACCAACC+TGG - chr4_2:14533040-14533059 None:intergenic 30.0%
! GAAAGAATAGGCAAACATTT+TGG - chr4_2:14533396-14533415 None:intergenic 30.0%
CACTATGATGTTGAAAGAAT+AGG - chr4_2:14533408-14533427 None:intergenic 30.0%
GAAGCTAATAGAAGAGCAAA+AGG - chr4_2:14528028-14528047 None:intergenic 35.0%
ACACTACACCTTCTTACATA+AGG - chr4_2:14528220-14528239 None:intergenic 35.0%
ATTAATTGTTGGTCCTGGTA+AGG - chr4_2:14528870-14528889 None:intergenic 35.0%
CCAATGAAGAGTAAGATTGT+AGG - chr4_2:14528939-14528958 None:intergenic 35.0%
CCTACAATCTTACTCTTCAT+TGG + chr4_2:14528936-14528955 Msa0590810:CDS 35.0%
TACAAGAAACGTTTAACAGG+AGG - chr4_2:14529060-14529079 None:intergenic 35.0%
TCTTATAGTTGATCGACCAT+CGG + chr4_2:14529560-14529579 Msa0590810:intron 35.0%
TCATAAAAGAGGGTGAAGTT+TGG + chr4_2:14529728-14529747 Msa0590810:intron 35.0%
!! TTTTTAACTCCTTGGTCAGA+TGG + chr4_2:14530219-14530238 Msa0590810:CDS 35.0%
ACCTACCAATTTAACCTAAC+TGG + chr4_2:14530288-14530307 Msa0590810:CDS 35.0%
TCCAGTTAGGTTAAATTGGT+AGG - chr4_2:14530292-14530311 None:intergenic 35.0%
CTTGTCCAGTTAGGTTAAAT+TGG - chr4_2:14530296-14530315 None:intergenic 35.0%
ATTTAACCTAACTGGACAAG+AGG + chr4_2:14530296-14530315 Msa0590810:CDS 35.0%
TTTAACCTAACTGGACAAGA+GGG + chr4_2:14530297-14530316 Msa0590810:CDS 35.0%
TAGTCGTGGCTTAATGATAA+GGG - chr4_2:14530376-14530395 None:intergenic 35.0%
! TCTTGATAAACCTTTGGGAA+GGG - chr4_2:14530411-14530430 None:intergenic 35.0%
! TTCTTGATAAACCTTTGGGA+AGG - chr4_2:14530412-14530431 None:intergenic 35.0%
GGACTTCTTGATAAACCTTT+GGG - chr4_2:14530416-14530435 None:intergenic 35.0%
ATAATTAAATTGCAGGGGAG+TGG + chr4_2:14530794-14530813 Msa0590810:intron 35.0%
TGAGAGAAATGCAACAGATA+CGG + chr4_2:14530840-14530859 Msa0590810:CDS 35.0%
TTCCCCTTTAAGTGAATAAG+TGG + chr4_2:14531197-14531216 Msa0590810:intron 35.0%
CTCCACTTATTCACTTAAAG+GGG - chr4_2:14531202-14531221 None:intergenic 35.0%
ATTATCTTTGGTAAGGAGGT+TGG - chr4_2:14531323-14531342 None:intergenic 35.0%
! GTGATGTTAACTTGAGATCT+TGG - chr4_2:14531375-14531394 None:intergenic 35.0%
TAGCTACAACTGTGAAACTA+TGG - chr4_2:14531643-14531662 None:intergenic 35.0%
TAGTTTCACAGTTGTAGCTA+TGG + chr4_2:14531643-14531662 Msa0590810:CDS 35.0%
ACACTATAACACTGATGTTG+TGG + chr4_2:14531682-14531701 Msa0590810:CDS 35.0%
!! AGTTTTCAAGAGAACGTCAA+CGG - chr4_2:14531726-14531745 None:intergenic 35.0%
ATAAACAACAACTCCTCTAG+TGG - chr4_2:14531822-14531841 None:intergenic 35.0%
!! AGAGGAGTTGTTGTTTATGA+TGG + chr4_2:14531824-14531843 Msa0590810:CDS 35.0%
! GCATTATAGGTGCTTTTGTT+TGG - chr4_2:14531856-14531875 None:intergenic 35.0%
TCAGGCATAATTGGCATTAT+AGG - chr4_2:14531869-14531888 None:intergenic 35.0%
TCATGGTTATCAGGCATAAT+TGG - chr4_2:14531878-14531897 None:intergenic 35.0%
TTTAACAACTGGTCCACATT+GGG + chr4_2:14532463-14532482 Msa0590810:CDS 35.0%
ATGTGTTCATCTACTTTACG+TGG - chr4_2:14532497-14532516 None:intergenic 35.0%
! ATGAATCTTTTGCTCTTCCT+AGG + chr4_2:14532612-14532631 Msa0590810:CDS 35.0%
! TGAATCTTTTGCTCTTCCTA+GGG + chr4_2:14532613-14532632 Msa0590810:CDS 35.0%
GATCAACATTAGCCAATGAA+GGG - chr4_2:14532739-14532758 None:intergenic 35.0%
! GTCTTCACCTTAGTTGATTT+AGG - chr4_2:14532785-14532804 None:intergenic 35.0%
!!! AACTTTCATGTTTTGGCACA+AGG + chr4_2:14532893-14532912 Msa0590810:CDS 35.0%
! AAACTTTGGTTCATCTCTAG+TGG - chr4_2:14532936-14532955 None:intergenic 35.0%
TTCAAGCAAATAATCCAGGT+TGG + chr4_2:14533023-14533042 Msa0590810:intron 35.0%
!! TTGGCTTGTACATTGTCATT+TGG + chr4_2:14533142-14533161 Msa0590810:CDS 35.0%
GAATAGTGAAGTCTTCAACC+TGG - chr4_2:14528805-14528824 None:intergenic 40.0%
ACCAACAATTAATGCTCGAG+AGG + chr4_2:14528877-14528896 Msa0590810:CDS 40.0%
CCAACAATTAATGCTCGAGA+GGG + chr4_2:14528878-14528897 Msa0590810:CDS 40.0%
TCTTGCTAACGAGTGTTCTA+AGG + chr4_2:14529397-14529416 Msa0590810:intron 40.0%
GGAGAAAGAATTAAGACCGA+TGG - chr4_2:14529579-14529598 None:intergenic 40.0%
CTAGTCGTGGCTTAATGATA+AGG - chr4_2:14530377-14530396 None:intergenic 40.0%
CTTATCATTAAGCCACGACT+AGG + chr4_2:14530375-14530394 Msa0590810:CDS 40.0%
! CTTGATAAACCTTTGGGAAG+GGG - chr4_2:14530410-14530429 None:intergenic 40.0%
GGGACTTCTTGATAAACCTT+TGG - chr4_2:14530417-14530436 None:intergenic 40.0%
TTGCATGCATTCACGCAATT+AGG + chr4_2:14530531-14530550 Msa0590810:intron 40.0%
GAGAGAAATGCAACAGATAC+GGG + chr4_2:14530841-14530860 Msa0590810:CDS 40.0%
ACATTGCAGTATGAAGAGGT+TGG - chr4_2:14531240-14531259 None:intergenic 40.0%
AGGTTGGATTATGAAGAGGT+TGG - chr4_2:14531307-14531326 None:intergenic 40.0%
AAGGAGGTTGGATTATGAAG+AGG - chr4_2:14531311-14531330 None:intergenic 40.0%
TACAAGCTCAAAGTGAAGCA+AGG + chr4_2:14531551-14531570 Msa0590810:CDS 40.0%
TGGTTCCAACACTTGAAGAA+CGG - chr4_2:14531790-14531809 None:intergenic 40.0%
ACCACTAACATAACCACTAG+AGG + chr4_2:14531806-14531825 Msa0590810:CDS 40.0%
TCCTCTAGTGGTTATGTTAG+TGG - chr4_2:14531810-14531829 None:intergenic 40.0%
!! TATAGGTGCTTTTGTTTGGG+AGG - chr4_2:14531852-14531871 None:intergenic 40.0%
GTTGGTGTATCATGGTTATC+AGG - chr4_2:14531887-14531906 None:intergenic 40.0%
GTGTAAAACTTGTGAGCTGT+TGG - chr4_2:14531905-14531924 None:intergenic 40.0%
GTTTAACAACTGGTCCACAT+TGG + chr4_2:14532462-14532481 Msa0590810:CDS 40.0%
TCATCTACTTTACGTGGAAC+TGG - chr4_2:14532491-14532510 None:intergenic 40.0%
TTTAGTACAACACAACGACC+TGG - chr4_2:14532566-14532585 None:intergenic 40.0%
! GAATCTTTTGCTCTTCCTAG+GGG + chr4_2:14532614-14532633 Msa0590810:CDS 40.0%
GGATCAACATTAGCCAATGA+AGG - chr4_2:14532740-14532759 None:intergenic 40.0%
GATTTAGGTGCAAATGCAAG+TGG - chr4_2:14532770-14532789 None:intergenic 40.0%
!!! CATGTTTTGGCACAAGGTTT+TGG + chr4_2:14532899-14532918 Msa0590810:CDS 40.0%
ATTTGGAAACACACTTGCCA+TGG + chr4_2:14533159-14533178 Msa0590810:CDS 40.0%
TTTGGAAACACACTTGCCAT+GGG + chr4_2:14533160-14533179 Msa0590810:CDS 40.0%
!!! ATGGCTTTTGAGGTTGAGAA+TGG + chr4_2:14533191-14533210 Msa0590810:CDS 40.0%
!!! TGGCTTTTGAGGTTGAGAAT+GGG + chr4_2:14533192-14533211 Msa0590810:CDS 40.0%
CAACACTTAGGAAGATTAGC+TGG - chr4_2:14533248-14533267 None:intergenic 40.0%
ATTTGCTTCTGCTGCTATCG+TGG + chr4_2:14528051-14528070 Msa0590810:CDS 45.0%
TCCTGGTAAGGTTCCATTGA+CGG - chr4_2:14528858-14528877 None:intergenic 45.0%
CGAGCATTAATTGTTGGTCC+TGG - chr4_2:14528875-14528894 None:intergenic 45.0%
CCCTCTCGAGCATTAATTGT+TGG - chr4_2:14528881-14528900 None:intergenic 45.0%
TATTCTGGACCATCTGACCA+AGG - chr4_2:14530231-14530250 None:intergenic 45.0%
!! GGGCATTGGGTTACATATTC+TGG - chr4_2:14530246-14530265 None:intergenic 45.0%
! ACCAGATGGTATTGGGCATT+GGG - chr4_2:14530259-14530278 None:intergenic 45.0%
!! GCTTAATGATAAGGGCACCA+TGG - chr4_2:14530368-14530387 None:intergenic 45.0%
CAAGAAGTCCCCATATTGCT+TGG + chr4_2:14530426-14530445 Msa0590810:CDS 45.0%
ATTAAATTGCAGGGGAGTGG+TGG + chr4_2:14530797-14530816 Msa0590810:intron 45.0%
TCAAGAGAACGTCAACGGTT+TGG - chr4_2:14531721-14531740 None:intergenic 45.0%
CCAACACTTGAAGAACGGTA+TGG - chr4_2:14531785-14531804 None:intergenic 45.0%
CCATACCGTTCTTCAAGTGT+TGG + chr4_2:14531782-14531801 Msa0590810:CDS 45.0%
TGTGAGCTGTTGGTGTATCA+TGG - chr4_2:14531895-14531914 None:intergenic 45.0%
CTAGAGCAATGCATCAATCC+AGG + chr4_2:14532539-14532558 Msa0590810:CDS 45.0%
ATTGAGAAACCTCTACCCCT+AGG - chr4_2:14532632-14532651 None:intergenic 45.0%
GGGTAGAGGTTTCTCAATGT+TGG + chr4_2:14532634-14532653 Msa0590810:CDS 45.0%
GCAAATGCAAGTGGCTTAGA+AGG - chr4_2:14532761-14532780 None:intergenic 45.0%
TAACACCATTTCTGTGCCAG+TGG + chr4_2:14532979-14532998 Msa0590810:CDS 45.0%
AACACCATTTCTGTGCCAGT+GGG + chr4_2:14532980-14532999 Msa0590810:CDS 45.0%
TTGGAAACACACTTGCCATG+GGG + chr4_2:14533161-14533180 Msa0590810:CDS 45.0%
! TTAGGAAGATTAGCTGGTGG+AGG - chr4_2:14533242-14533261 None:intergenic 45.0%
! CACTTAGGAAGATTAGCTGG+TGG - chr4_2:14533245-14533264 None:intergenic 45.0%
!! TTATTTAATAATTTATGAAT+TGG - chr4_2:14529440-14529459 None:intergenic 5.0%
!! AAACAAAATTATAAATAATA+TGG + chr4_2:14529657-14529676 Msa0590810:intron 5.0%
TTGACGGCAGTGATAACCTG+TGG - chr4_2:14528842-14528861 None:intergenic 50.0%
CAGGTTATCACTGCCGTCAA+TGG + chr4_2:14528842-14528861 Msa0590810:CDS 50.0%
ACCCAATGCCCAATACCATC+TGG + chr4_2:14530255-14530274 Msa0590810:CDS 50.0%
! CACCAGATGGTATTGGGCAT+TGG - chr4_2:14530260-14530279 None:intergenic 50.0%
CAATGCCCAATACCATCTGG+TGG + chr4_2:14530258-14530277 Msa0590810:CDS 50.0%
AGCTGCCACCAGATGGTATT+GGG - chr4_2:14530266-14530285 None:intergenic 50.0%
TAGCTGCCACCAGATGGTAT+TGG - chr4_2:14530267-14530286 None:intergenic 50.0%
TAGGTATAGCTGCCACCAGA+TGG - chr4_2:14530273-14530292 None:intergenic 50.0%
GTGTACCCTCTTGTCCAGTT+AGG - chr4_2:14530305-14530324 None:intergenic 50.0%
! CTGGACAAGAGGGTACACTT+TGG + chr4_2:14530307-14530326 Msa0590810:CDS 50.0%
! GACAAGAGGGTACACTTTGG+TGG + chr4_2:14530310-14530329 Msa0590810:CDS 50.0%
! TTCCTACGTGCTACTGTCCA+TGG + chr4_2:14530348-14530367 Msa0590810:CDS 50.0%
ATGCATTTGACTCCGCTGGT+GGG - chr4_2:14530870-14530889 None:intergenic 50.0%
GTTGATGCATTTGACTCCGC+TGG - chr4_2:14530874-14530893 None:intergenic 50.0%
GTGCGTGTGTGTCAGATTCA+TGG - chr4_2:14532243-14532262 None:intergenic 50.0%
CAATGCATCAATCCAGGACC+AGG + chr4_2:14532545-14532564 Msa0590810:CDS 50.0%
TTTGCTCTTCCTAGGGGTAG+AGG + chr4_2:14532620-14532639 Msa0590810:CDS 50.0%
TGGAAACACACTTGCCATGG+GGG + chr4_2:14533162-14533181 Msa0590810:CDS 50.0%
AAAGCCATTGCTAACCCCCA+TGG - chr4_2:14533179-14533198 None:intergenic 50.0%
!! GGGGTTAGCAATGGCTTTTG+AGG + chr4_2:14533181-14533200 Msa0590810:CDS 50.0%
GTTGAGAATGGGCCTACTCT+TGG + chr4_2:14533203-14533222 Msa0590810:CDS 50.0%
GGCACTGTTGTTCCAAGAGT+AGG - chr4_2:14533218-14533237 None:intergenic 50.0%
CGGCAGTGATAACCTGTGGA+CGG - chr4_2:14528838-14528857 None:intergenic 55.0%
GCCGTCAATGGAACCTTACC+AGG + chr4_2:14528854-14528873 Msa0590810:CDS 55.0%
CACCATGGACAGTAGCACGT+AGG - chr4_2:14530353-14530372 None:intergenic 55.0%
TCGCTCATACCCCTTCCCAA+AGG + chr4_2:14530398-14530417 Msa0590810:CDS 55.0%
AGATACGGGTAACCCACCAG+CGG + chr4_2:14530855-14530874 Msa0590810:CDS 55.0%
GATGCATTTGACTCCGCTGG+TGG - chr4_2:14530871-14530890 None:intergenic 55.0%
! AGAACGTCAACGGTTTGGCC+AGG - chr4_2:14531716-14531735 None:intergenic 55.0%
CGTGGAACTGGAACCCAATG+TGG - chr4_2:14532479-14532498 None:intergenic 55.0%
ACAACACAACGACCTGGTCC+TGG - chr4_2:14532560-14532579 None:intergenic 55.0%
ACCATTTCTGTGCCAGTGGG+AGG + chr4_2:14532983-14533002 Msa0590810:CDS 55.0%
TCCTCCCACTGGCACAGAAA+TGG - chr4_2:14532987-14533006 None:intergenic 55.0%
TTTCTGTGCCAGTGGGAGGA+TGG + chr4_2:14532987-14533006 Msa0590810:CDS 55.0%
TTCTGTGCCAGTGGGAGGAT+GGG + chr4_2:14532988-14533007 Msa0590810:CDS 55.0%
ATAACAGCCCATCCTCCCAC+TGG - chr4_2:14532998-14533017 None:intergenic 55.0%
! CTTGCCATGGGGGTTAGCAA+TGG + chr4_2:14533172-14533191 Msa0590810:CDS 55.0%
! GGAAGATTAGCTGGTGGAGG+AGG - chr4_2:14533239-14533258 None:intergenic 55.0%
TCAGCGATTGTGCCGTCCAC+AGG + chr4_2:14528823-14528842 Msa0590810:CDS 60.0%
GGGTATGAGCGACCTAGTCG+TGG - chr4_2:14530390-14530409 None:intergenic 60.0%
GATGTTGTGGTGCTTGCGCC+TGG + chr4_2:14531695-14531714 Msa0590810:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 14527976 14533478 14527976 ID=Msa0590810;Name=Msa0590810
chr4_2 mRNA 14527976 14533478 14527976 ID=Msa0590810-mRNA-1;Parent=Msa0590810;Name=Msa0590810-mRNA-1;_AED=0.10;_eAED=0.10;_QI=19|1|1|1|1|1|7|210|572
chr4_2 exon 14527976 14528087 14527976 ID=Msa0590810-mRNA-1:exon:4177;Parent=Msa0590810-mRNA-1
chr4_2 exon 14528806 14528957 14528806 ID=Msa0590810-mRNA-1:exon:4178;Parent=Msa0590810-mRNA-1
chr4_2 exon 14530203 14530447 14530203 ID=Msa0590810-mRNA-1:exon:4179;Parent=Msa0590810-mRNA-1
chr4_2 exon 14530809 14530937 14530809 ID=Msa0590810-mRNA-1:exon:4180;Parent=Msa0590810-mRNA-1
chr4_2 exon 14531546 14531935 14531546 ID=Msa0590810-mRNA-1:exon:4181;Parent=Msa0590810-mRNA-1
chr4_2 exon 14532462 14533040 14532462 ID=Msa0590810-mRNA-1:exon:4182;Parent=Msa0590810-mRNA-1
chr4_2 exon 14533138 14533478 14533138 ID=Msa0590810-mRNA-1:exon:4183;Parent=Msa0590810-mRNA-1
chr4_2 five_prime_UTR 14527976 14527994 14527976 ID=Msa0590810-mRNA-1:five_prime_utr;Parent=Msa0590810-mRNA-1
chr4_2 CDS 14527995 14528087 14527995 ID=Msa0590810-mRNA-1:cds;Parent=Msa0590810-mRNA-1
chr4_2 CDS 14528806 14528957 14528806 ID=Msa0590810-mRNA-1:cds;Parent=Msa0590810-mRNA-1
chr4_2 CDS 14530203 14530447 14530203 ID=Msa0590810-mRNA-1:cds;Parent=Msa0590810-mRNA-1
chr4_2 CDS 14530809 14530937 14530809 ID=Msa0590810-mRNA-1:cds;Parent=Msa0590810-mRNA-1
chr4_2 CDS 14531546 14531935 14531546 ID=Msa0590810-mRNA-1:cds;Parent=Msa0590810-mRNA-1
chr4_2 CDS 14532462 14533040 14532462 ID=Msa0590810-mRNA-1:cds;Parent=Msa0590810-mRNA-1
chr4_2 CDS 14533138 14533268 14533138 ID=Msa0590810-mRNA-1:cds;Parent=Msa0590810-mRNA-1
chr4_2 three_prime_UTR 14533269 14533478 14533269 ID=Msa0590810-mRNA-1:three_prime_utr;Parent=Msa0590810-mRNA-1
Gene Sequence

>Msa0590810

ATGACACATTTTATGTTTTCTCTAGCATGGCCTTTTGCTCTTCTATTAGCTTCTTCATTTGCTTCTGCTGCTATCGTGGAACATACTTTCAATGTTGAAGACTTCACTATTCAGCGATTGTGCCGTCCACAGGTTATCACTGCCGTCAATGGAACCTTACCAGGACCAACAATTAATGCTCGAGAGGGTGACACTGTTATCATTCATGCATTCAACAAATCACCCTACAATCTTACTCTTCATTGGCATGGAATCATTCAATTTTTAACTCCTTGGTCAGATGGTCCAGAATATGTAACCCAATGCCCAATACCATCTGGTGGCAGCTATACCTACCAATTTAACCTAACTGGACAAGAGGGTACACTTTGGTGGCATGCACACTCTTCTTTCCTACGTGCTACTGTCCATGGTGCCCTTATCATTAAGCCACGACTAGGTCGCTCATACCCCTTCCCAAAGGTTTATCAAGAAGTCCCCATATTGCTTGGGGAGTGGTGGAATGCTAATGTTGAAGAAGTTGAGAGAAATGCAACAGATACGGGTAACCCACCAGCGGAGTCAAATGCATCAACAATTAATGGCTTATCTGGTGACTTGTATAACTGCTCCCAAGATCAGAATTACAAGCTCAAAGTGAAGCAAGGAAAAACCTACTTACTACGCATTATCAATGCTGCACTCAATGAACAACATTTCTTCAAGATAGCAAACCATAGTTTCACAGTTGTAGCTATGGATGCTATCTACACAGAACACTATAACACTGATGTTGTGGTGCTTGCGCCTGGCCAAACCGTTGACGTTCTCTTGAAAACTAACCAAGTTGTTGATTCTTATTACATGGTTATGACACCATACCGTTCTTCAAGTGTTGGAACCACTAACATAACCACTAGAGGAGTTGTTGTTTATGATGGTGCCTCCCAAACAAAAGCACCTATAATGCCAATTATGCCTGATAACCATGATACACCAACAGCTCACAAGTTTTACACCAATGTTACAGGTTTAACAACTGGTCCACATTGGGTTCCAGTTCCACGTAAAGTAGATGAACACATGTTCATAACTTTTGGTATTGGTCTAGAGCAATGCATCAATCCAGGACCAGGTCGTTGTGTTGTACTAAACTCTCGACTTTCTGCCAACATGAACAATGAATCTTTTGCTCTTCCTAGGGGTAGAGGTTTCTCAATGTTGGAAGCATTTTACAACAATATTAGTGGTGTGTACACAAAAGATTTTCCAAATCAACCACCATTTGAATTTAATTATACTGACCCTTCATTGGCTAATGTTGATCCTTCTAAGCCACTTGCATTTGCACCTAAATCAACTAAGGTGAAGACTTTGAAGTTTAATTCAACGATTGAGATTGTGCTACAAAATACAGCTATTCTTGGGGCAGAGAATCATCCTATTCATATTCATGGGTTTAACTTTCATGTTTTGGCACAAGGTTTTGGAAATTATAATGCCACTAGAGATGAACCAAAGTTTAACTTTGTTAATCCTCAAATGCGTAACACCATTTCTGTGCCAGTGGGAGGATGGGCTGTTATTAGATTTCAAGCAAATAATCCAGGTATTTGGCTTGTACATTGTCATTTGGAAACACACTTGCCATGGGGGTTAGCAATGGCTTTTGAGGTTGAGAATGGGCCTACTCTTGGAACAACAGTGCCTCCTCCACCAGCTAATCTTCCTAAGTGTTGA
Protein sequence

>Msa0590810

MTHFMFSLAWPFALLLASSFASAAIVEHTFNVEDFTIQRLCRPQVITAVNGTLPGPTINAREGDTVIIHAFNKSPYNLTLHWHGIIQFLTPWSDGPEYVTQCPIPSGGSYTYQFNLTGQEGTLWWHAHSSFLRATVHGALIIKPRLGRSYPFPKVYQEVPILLGEWWNANVEEVERNATDTGNPPAESNASTINGLSGDLYNCSQDQNYKLKVKQGKTYLLRIINAALNEQHFFKIANHSFTVVAMDAIYTEHYNTDVVVLAPGQTVDVLLKTNQVVDSYYMVMTPYRSSSVGTTNITTRGVVVYDGASQTKAPIMPIMPDNHDTPTAHKFYTNVTGLTTGPHWVPVPRKVDEHMFITFGIGLEQCINPGPGRCVVLNSRLSANMNNESFALPRGRGFSMLEAFYNNISGVYTKDFPNQPPFEFNYTDPSLANVDPSKPLAFAPKSTKVKTLKFNSTIEIVLQNTAILGAENHPIHIHGFNFHVLAQGFGNYNATRDEPKFNFVNPQMRNTISVPVGGWAVIRFQANNPGIWLVHCHLETHLPWGLAMAFEVENGPTLGTTVPPPPANLPKC*