AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0669350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0669350 MtrunA17_Chr4g0008521 95.804 572 24 0 1 572 1 572 0.0 1123
Msa0669350 MtrunA17_Chr7g0240971 62.609 575 207 5 1 572 1 570 0.0 717
Msa0669350 MtrunA17_Chr7g0240991 57.544 570 228 9 10 572 9 571 0.0 683
Msa0669350 MtrunA17_Chr5g0404361 45.760 566 288 5 8 572 64 611 0.0 536
Msa0669350 MtrunA17_Chr5g0437681 47.803 569 274 9 14 572 12 567 0.0 532
Msa0669350 MtrunA17_Chr5g0431581 47.331 562 280 8 14 572 12 560 0.0 524
Msa0669350 MtrunA17_Chr3g0114211 48.155 569 274 9 12 572 15 570 0.0 523
Msa0669350 MtrunA17_Chr5g0404341 44.326 564 295 6 12 572 19 566 9.69e-176 509
Msa0669350 MtrunA17_Chr5g0429511 44.565 552 291 4 21 572 22 558 4.57e-173 501
Msa0669350 MtrunA17_Chr4g0005921 45.263 570 293 5 8 572 2 557 2.07e-172 500
Msa0669350 MtrunA17_Chr3g0104061 44.283 551 291 4 22 572 22 556 2.57e-172 499
Msa0669350 MtrunA17_Chr7g0238981 45.098 561 278 7 27 572 48 593 1.22e-171 499
Msa0669350 MtrunA17_Chr7g0238941 45.234 577 295 6 4 572 15 578 5.93e-171 497
Msa0669350 MtrunA17_Chr7g0237491 45.299 585 289 10 7 572 8 580 5.86e-168 489
Msa0669350 MtrunA17_Chr5g0436461 43.881 572 292 7 4 572 9 554 4.78e-166 483
Msa0669350 MtrunA17_Chr7g0237441 44.696 575 286 9 15 572 21 580 3.90e-165 482
Msa0669350 MtrunA17_Chr7g0238351 44.779 565 289 7 21 572 27 581 1.55e-164 481
Msa0669350 MtrunA17_Chr4g0031901 45.191 551 281 8 13 555 31 568 1.92e-164 481
Msa0669350 MtrunA17_Chr2g0278811 40.464 603 310 9 5 572 19 607 1.38e-160 471
Msa0669350 MtrunA17_Chr8g0346581 40.693 548 310 8 28 572 36 571 1.50e-149 442
Msa0669350 MtrunA17_Chr3g0133861 41.228 342 182 11 228 562 1 330 5.89e-75 241
Msa0669350 MtrunA17_Chr3g0133871 47.959 196 101 1 12 206 17 212 2.29e-61 202
Msa0669350 MtrunA17_Chr8g0382691 30.404 569 312 20 21 551 29 551 1.26e-60 211
Msa0669350 MtrunA17_Chr3g0118321 28.720 578 351 17 1 550 1 545 9.57e-55 194
Msa0669350 MtrunA17_Chr3g0118331 28.265 559 334 17 23 550 22 544 5.48e-48 176
Msa0669350 MtrunA17_Chr5g0436431 37.190 242 131 5 292 530 5 228 2.71e-44 157
Msa0669350 MtrunA17_Chr4g0056511 26.620 571 371 13 14 572 15 549 2.22e-40 155
Msa0669350 MtrunA17_Chr3g0079321 25.473 581 394 11 1 572 1 551 1.86e-39 152
Msa0669350 MtrunA17_Chr4g0038671 25.599 543 338 18 11 538 7 498 3.67e-31 127
Msa0669350 MtrunA17_Chr3g0143671 25.235 531 340 14 49 572 53 533 6.13e-31 127
Msa0669350 MtrunA17_Chr8g0374271 25.875 514 317 14 29 532 34 493 2.45e-30 125
Msa0669350 MtrunA17_Chr8g0374261 25.292 514 320 16 29 532 32 491 4.05e-30 124
Msa0669350 MtrunA17_Chr3g0121161 24.638 552 370 16 8 554 48 558 3.49e-29 122
Msa0669350 MtrunA17_Chr3g0121171 24.319 551 373 15 8 554 1158 1668 9.04e-29 122
Msa0669350 MtrunA17_Chr3g0121171 24.457 552 371 16 8 554 583 1093 1.14e-28 122
Msa0669350 MtrunA17_Chr3g0121171 24.304 539 364 15 20 554 20 518 1.53e-27 118
Msa0669350 MtrunA17_Chr3g0121171 23.732 552 366 17 8 554 1733 2234 3.40e-23 105
Msa0669350 MtrunA17_Chr4g0024861 24.457 552 360 17 28 572 29 530 9.53e-29 120
Msa0669350 MtrunA17_Chr5g0402831 25.543 552 350 16 28 572 33 530 1.34e-28 119
Msa0669350 MtrunA17_Chr3g0121151 24.549 554 368 17 8 554 48 558 1.44e-28 120
Msa0669350 MtrunA17_Chr2g0322441 25.000 552 357 17 28 572 32 533 1.57e-28 119
Msa0669350 MtrunA17_Chr1g0157341 23.774 530 349 14 49 572 52 532 6.37e-27 114
Msa0669350 MtrunA17_Chr4g0043261 22.951 549 375 14 8 552 7 511 1.26e-26 114
Msa0669350 MtrunA17_Chr7g0234061 25.189 528 341 18 19 532 22 509 2.85e-26 113
Msa0669350 MtrunA17_Chr4g0043251 24.419 516 342 16 43 552 44 517 9.70e-24 105
Msa0669350 MtrunA17_Chr3g0118341 36.000 150 92 4 28 175 28 175 5.25e-22 94.7
Msa0669350 MtrunA17_Chr4g0075071 24.848 495 307 13 49 532 54 494 1.65e-21 98.2
Msa0669350 MtrunA17_Chr3g0118351 28.135 327 206 11 238 550 5 316 5.27e-20 91.7
Msa0669350 MtrunA17_Chr4g0024641 24.000 450 286 17 92 535 17 416 4.37e-15 79.0
Msa0669350 MtrunA17_Chr1g0197311 28.641 206 111 6 44 217 42 243 6.47e-13 68.9
Msa0669350 MtrunA17_Chr3g0118931 39.130 69 42 0 482 550 10 78 3.44e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0669350 AT3G09220.1 58.228 553 216 7 8 555 8 550 0.0 671
Msa0669350 AT5G01050.1 54.867 565 241 5 12 572 14 568 0.0 649
Msa0669350 AT5G01040.1 53.391 575 253 8 1 572 4 566 0.0 639
Msa0669350 AT3G09220.2 58.225 462 178 7 99 555 2 453 0.0 554
Msa0669350 AT5G05390.1 47.719 570 284 6 6 572 7 565 0.0 546
Msa0669350 AT2G40370.1 46.967 577 278 9 13 572 15 580 0.0 527
Msa0669350 AT5G03260.1 46.491 570 287 5 4 572 5 557 2.81e-180 520
Msa0669350 AT2G30210.1 46.367 578 285 8 6 572 7 570 1.93e-179 518
Msa0669350 AT5G58910.2 46.561 567 283 6 12 572 15 567 2.89e-176 510
Msa0669350 AT5G58910.3 46.737 567 281 7 12 572 15 566 1.98e-175 508
Msa0669350 AT2G38080.1 44.406 572 300 6 1 572 5 558 6.16e-175 506
Msa0669350 AT5G07130.1 46.875 576 280 11 11 572 6 569 5.69e-174 504
Msa0669350 AT2G29130.1 45.629 572 295 6 8 572 11 573 8.17e-173 502
Msa0669350 AT5G01190.1 45.374 562 292 4 12 572 11 558 1.66e-170 495
Msa0669350 AT5G58910.1 46.753 539 261 6 39 572 6 523 1.15e-168 489
Msa0669350 AT5G60020.1 44.097 576 296 6 13 572 12 577 3.39e-167 488
Msa0669350 AT5G60020.3 45.290 552 276 6 37 572 4 545 1.11e-166 485
Msa0669350 AT5G09360.1 42.984 563 300 9 14 572 24 569 1.53e-165 483
Msa0669350 AT5G01190.2 44.840 562 281 5 12 572 11 544 2.36e-164 479
Msa0669350 AT1G18140.1 43.137 561 289 9 30 572 33 581 4.83e-156 459
Msa0669350 AT2G46570.1 42.626 556 298 9 23 572 29 569 2.17e-153 452
Msa0669350 AT5G60020.2 43.446 534 276 6 13 530 12 535 3.77e-148 438
Msa0669350 AT5G07130.2 47.262 493 237 10 91 572 4 484 1.04e-146 432
Msa0669350 AT5G07130.3 47.262 493 237 10 91 572 4 484 1.04e-146 432
Msa0669350 AT5G48100.1 40.429 559 300 12 12 555 6 546 8.87e-143 425
Msa0669350 AT2G46570.2 42.485 499 266 9 80 572 1 484 5.41e-133 397
Msa0669350 AT5G21100.1 29.217 575 343 16 21 567 19 557 4.39e-62 215
Msa0669350 AT4G39830.1 29.088 581 328 19 13 551 23 561 2.27e-60 210
Msa0669350 AT5G21105.1 27.592 569 354 13 10 550 20 558 3.62e-57 202
Msa0669350 AT5G21105.3 26.958 549 343 13 10 530 20 538 4.03e-48 176
Msa0669350 AT4G39830.2 28.543 508 280 18 85 551 1 466 3.90e-45 167
Msa0669350 AT4G39830.3 28.628 503 276 18 90 551 4 464 1.21e-44 165
Msa0669350 AT4G39830.4 28.008 507 271 17 85 551 1 453 7.75e-42 157
Msa0669350 AT4G25240.2 26.876 573 370 16 12 572 15 550 9.56e-41 156
Msa0669350 AT4G25240.1 26.876 573 370 16 12 572 15 550 9.56e-41 156
Msa0669350 AT5G51480.1 25.569 571 377 14 14 572 16 550 3.52e-40 154
Msa0669350 AT4G12420.1 24.764 529 358 14 12 532 11 507 8.79e-35 139
Msa0669350 AT4G12420.2 24.764 529 358 14 12 532 11 507 8.79e-35 139
Msa0669350 AT1G41830.1 25.391 575 358 17 14 572 12 531 1.67e-33 134
Msa0669350 AT1G76160.1 25.130 577 371 18 7 572 4 530 3.56e-32 130
Msa0669350 AT1G21860.1 25.376 532 338 17 14 538 17 496 5.00e-32 130
Msa0669350 AT5G48450.2 23.836 537 367 15 14 547 17 514 1.13e-31 130
Msa0669350 AT4G37160.1 24.180 579 373 15 4 572 12 534 1.85e-31 129
Msa0669350 AT5G48450.1 23.836 537 367 15 14 547 17 514 2.58e-31 129
Msa0669350 AT1G75790.1 24.564 574 371 19 14 572 15 541 1.37e-30 126
Msa0669350 AT1G21850.1 25.151 497 313 16 49 538 52 496 7.62e-30 124
Msa0669350 AT2G23630.2 24.771 545 364 13 29 572 38 537 1.82e-29 122
Msa0669350 AT2G23630.1 24.635 548 361 14 29 572 33 532 1.96e-29 122
Msa0669350 AT5G66920.1 23.835 558 354 14 28 572 39 538 6.36e-29 121
Msa0669350 AT1G55560.1 30.612 294 194 5 28 320 28 312 2.64e-28 119
Msa0669350 AT3G13400.2 24.366 513 336 13 28 532 29 497 2.61e-27 116
Msa0669350 AT3G13400.1 24.366 513 336 13 28 532 29 497 2.61e-27 116
Msa0669350 AT4G22010.1 24.953 537 335 17 49 572 49 530 1.26e-26 114
Msa0669350 AT5G21105.2 25.364 343 227 5 211 550 51 367 2.67e-26 111
Msa0669350 AT4G37160.2 27.726 321 214 5 4 323 12 315 7.22e-26 111
Msa0669350 AT1G55570.1 24.105 531 355 12 12 532 8 500 7.55e-26 112
Msa0669350 AT3G13390.1 25.245 511 341 15 28 535 30 502 4.87e-25 109
Msa0669350 AT4G28090.1 23.800 500 321 16 43 532 45 494 7.07e-22 99.8
Msa0669350 AT4G38420.2 28.405 257 160 7 37 285 41 281 4.11e-18 87.8
Msa0669350 AT4G38420.3 28.405 257 160 7 37 285 41 281 8.93e-18 87.0
Msa0669350 AT4G38420.1 28.405 257 160 7 37 285 41 281 1.55e-17 86.3

Find 147 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATAAAATGTGTCATATT+TGG 0.185163 4_4:-11009356 None:intergenic
TTAAACATTATGCAGGTATT+TGG 0.193690 4_4:+11014522 Msa0669350:intron
GGGCATTGGGTTACATATTC+TGG 0.208610 4_4:-11011644 None:intergenic
AATTCAAATGGTGGTTGATT+TGG 0.228784 4_4:-11014100 None:intergenic
GAAAGAATAGGCAAACATTT+TGG 0.246426 4_4:-11014792 None:intergenic
AATTAACTCAAGAATTTATA+AGG 0.257962 4_4:-11014688 None:intergenic
TCATGGTTATCAGGCATAAT+TGG 0.277334 4_4:-11013274 None:intergenic
TGGGTTTAACTTTCATGTTT+TGG 0.277831 4_4:+11014285 Msa0669350:CDS
TCAATTTGATAAATGTTTAC+AGG 0.279128 4_4:-11014839 None:intergenic
GAACACATGTTCATAACTTT+TGG 0.285867 4_4:+11013908 Msa0669350:CDS
GTCTTCACCTTAGTTGATTT+AGG 0.296358 4_4:-11014181 None:intergenic
TCAACAATTAATGGCTTATC+TGG 0.303948 4_4:+11012290 Msa0669350:CDS
ACCTACCAATTTAACCTAAC+TGG 0.312872 4_4:+11011689 Msa0669350:CDS
CTTGTCCAGTTAGGTTAAAT+TGG 0.320988 4_4:-11011694 None:intergenic
TCAAATGCATCAACAATTAA+TGG 0.327150 4_4:+11012281 Msa0669350:CDS
GGGACTTCTTGATAAACCTT+TGG 0.333644 4_4:-11011815 None:intergenic
TCAGGCATAATTGGCATTAT+AGG 0.339643 4_4:-11013265 None:intergenic
CCTACAATCTTACTCTTCAT+TGG 0.348502 4_4:+11010305 Msa0669350:CDS
AATCATCCTATTCATATTCA+TGG 0.354176 4_4:+11014265 Msa0669350:CDS
CACCAGATGGTATTGGGCAT+TGG 0.364496 4_4:-11011658 None:intergenic
AGCTGCCACCAGATGGTATT+GGG 0.364532 4_4:-11011664 None:intergenic
CTACAAAATACAGCTATTCT+TGG 0.369525 4_4:+11014235 Msa0669350:CDS
TAATTATACTGACCCTTCAT+TGG 0.373770 4_4:+11014123 Msa0669350:CDS
TTGGCTTGTACATTGTCATT+TGG 0.376918 4_4:+11014541 Msa0669350:CDS
GCAAATGCAAGTGGCTTAGA+AGG 0.379879 4_4:-11014157 None:intergenic
GACGTTGTGGTGCTTGCGCC+TGG 0.380297 4_4:+11013094 Msa0669350:CDS
CTTATCATTAAGCCACGATT+AGG 0.380660 4_4:+11011776 Msa0669350:CDS
CCCTCTCGAGCATTAATTGT+TGG 0.386584 4_4:-11010247 None:intergenic
AAAACAATACCAAGCAATAT+GGG 0.389972 4_4:-11011836 None:intergenic
GTTGGTGTATCATGGTTATC+AGG 0.392956 4_4:-11013283 None:intergenic
AATTGAAGACTTACGATCTT+GGG 0.401325 4_4:-11012330 None:intergenic
ACCAGATGGTATTGGGCATT+GGG 0.403258 4_4:-11011657 None:intergenic
TCATCTACTTTACGTGGAAC+TGG 0.407962 4_4:-11013887 None:intergenic
TACAAAATACAGCTATTCTT+GGG 0.408559 4_4:+11014236 Msa0669350:CDS
TAATTGAAGACTTACGATCT+TGG 0.410547 4_4:-11012331 None:intergenic
TAGCTACAACTGTGAAACTA+TGG 0.411298 4_4:-11013039 None:intergenic
TCCTCCCACTGGCACAGAAA+TGG 0.414706 4_4:-11014383 None:intergenic
CTAATCGTGGCTTAATGATA+AGG 0.425517 4_4:-11011775 None:intergenic
TCCTGGTAAGGTTCCATTGA+CGG 0.426598 4_4:-11010224 None:intergenic
GTGTACCCTCTTGTCCAGTT+AGG 0.427115 4_4:-11011703 None:intergenic
TTTCTGTGCCAGTGGGAGGA+TGG 0.429026 4_4:+11014386 Msa0669350:CDS
TTAAACCCATGAATATGAAT+AGG 0.429980 4_4:-11014271 None:intergenic
GGACTTCTTGATAAACCTTT+GGG 0.434862 4_4:-11011814 None:intergenic
GTTGAGAATGGGCCTACTCT+TGG 0.437907 4_4:+11014602 Msa0669350:CDS
TCAAGAGAACGTCAACGGTT+TGG 0.441613 4_4:-11013117 None:intergenic
TTCCTACGTGCTACTGTCCA+TGG 0.442379 4_4:+11011749 Msa0669350:CDS
TTTGCTCTTCCTAGGGGTAG+AGG 0.443734 4_4:+11014019 Msa0669350:CDS
CCAATGAAGAGTAAGATTGT+AGG 0.448345 4_4:-11010305 None:intergenic
TCCTCTAGTGGTTATGTTAG+CGG 0.449947 4_4:-11013206 None:intergenic
GAAGCTAATAGAAGAGCAAA+AGG 0.450795 4_4:-11009392 None:intergenic
TTCTGTGCCAGTGGGAGGAT+GGG 0.454325 4_4:+11014387 Msa0669350:CDS
CAACACTTGGGAAGATTAGC+TGG 0.455119 4_4:-11014644 None:intergenic
CACTATGATGTTGAAAGAAT+AGG 0.456772 4_4:-11014804 None:intergenic
CAGGTTATCACTGCCGTCAA+TGG 0.458437 4_4:+11010211 Msa0669350:CDS
ATATATACAGGTTTAACAAC+TGG 0.461888 4_4:+11013851 Msa0669350:intron
TTTAACAACTGGTCCACATT+GGG 0.462574 4_4:+11013862 Msa0669350:CDS
ATTGATAATGCGTAGTAAGT+AGG 0.465795 4_4:-11012978 None:intergenic
AGAGGAGTTGTTGTTTATGA+TGG 0.466459 4_4:+11013223 Msa0669350:CDS
TTATATCATACCTGTAACAT+TGG 0.468461 4_4:-11013323 None:intergenic
ATTTGCACCTAAATCAACTA+AGG 0.469161 4_4:+11014174 Msa0669350:CDS
TAGCTGCCACCAGATGGTAT+TGG 0.469636 4_4:-11011665 None:intergenic
TCAGTATAATTAAATTCAAA+TGG 0.470555 4_4:-11014112 None:intergenic
CTTGATAAACCTTTGGGAAG+GGG 0.471972 4_4:-11011808 None:intergenic
GCCGTCAATGGAACCTTACC+AGG 0.472419 4_4:+11010223 Msa0669350:CDS
GGATTAACAAAGTTAAACTT+TGG 0.476313 4_4:-11014346 None:intergenic
AGTTATTGATTCTTATTACA+TGG 0.478409 4_4:+11013150 Msa0669350:CDS
ATTAAATTGCAGGGGAGTGG+TGG 0.479049 4_4:+11012198 Msa0669350:intron
GAAATGGTGTTACGCATTTG+AGG 0.480620 4_4:-11014367 None:intergenic
TGAGAGAAATGCAACAGATA+CGG 0.485761 4_4:+11012241 Msa0669350:CDS
ATCATCCTATTCATATTCAT+GGG 0.488322 4_4:+11014266 Msa0669350:CDS
ATTAATTGTTGGTCCTGGTA+AGG 0.491356 4_4:-11010236 None:intergenic
TAAATAATGTACTCAACACT+TGG 0.491896 4_4:-11014657 None:intergenic
CGAGCATTAATTGTTGGTCC+TGG 0.493195 4_4:-11010241 None:intergenic
GAGAGAAATGCAACAGATAC+GGG 0.494209 4_4:+11012242 Msa0669350:CDS
TCAGCGATTGTGCCGTCCAC+AGG 0.494835 4_4:+11010192 Msa0669350:CDS
GGGTAGAGGTTTCTCAATGT+TGG 0.495591 4_4:+11014033 Msa0669350:CDS
CAATTTGATAAATGTTTACA+GGG 0.497563 4_4:-11014838 None:intergenic
CAAGAAGTCCCCATATTGCT+TGG 0.500129 4_4:+11011827 Msa0669350:CDS
AGAACGTCAACGGTTTGGCC+AGG 0.501113 4_4:-11013112 None:intergenic
ATTTGGAAACACACTTGCCA+TGG 0.502328 4_4:+11014558 Msa0669350:CDS
TAGTTTCACAGTTGTAGCTA+TGG 0.504059 4_4:+11013042 Msa0669350:CDS
TGTGAGCTGTTGGTGTATCA+TGG 0.504650 4_4:-11013291 None:intergenic
TTTGGAAACACACTTGCCAT+GGG 0.506002 4_4:+11014559 Msa0669350:CDS
GGATCAACATTAGCCAATGA+AGG 0.506492 4_4:-11014136 None:intergenic
TAATCGTGGCTTAATGATAA+GGG 0.508095 4_4:-11011774 None:intergenic
ACAACACAACGACCTGGTCC+TGG 0.509215 4_4:-11013956 None:intergenic
ATTTGCTTCTGCTGCTATCG+TGG 0.515893 4_4:+11009418 Msa0669350:CDS
TTCTTGATAAACCTTTGGGA+AGG 0.519043 4_4:-11011810 None:intergenic
AAAAGATTCATTGTTCATGT+TGG 0.521543 4_4:-11013999 None:intergenic
GGAAGATTAGCTGGTGGAGG+AGG 0.523061 4_4:-11014635 None:intergenic
CAATGAAGAGTAAGATTGTA+GGG 0.523403 4_4:-11010304 None:intergenic
AGATTTCAAGCAAATAATCC+AGG 0.524094 4_4:+11014418 Msa0669350:CDS
ACCCAATGCCCAATACCATC+TGG 0.526254 4_4:+11011656 Msa0669350:CDS
ATAATTAAATTGCAGGGGAG+TGG 0.527515 4_4:+11012195 Msa0669350:intron
AAACTTTGGTTCATCTCTAG+TGG 0.533014 4_4:-11014332 None:intergenic
TCTTGATAAACCTTTGGGAA+GGG 0.536055 4_4:-11011809 None:intergenic
ACCAACAATTAATGCTCGAG+AGG 0.542459 4_4:+11010246 Msa0669350:CDS
TGTAATAAGAATCAATAACT+TGG 0.543985 4_4:-11013147 None:intergenic
CCATACCGTTCTTCAAGTGT+TGG 0.544154 4_4:+11013181 Msa0669350:CDS
TCCAGTTAGGTTAAATTGGT+AGG 0.550019 4_4:-11011690 None:intergenic
GTTTAACAACTGGTCCACAT+TGG 0.554162 4_4:+11013861 Msa0669350:CDS
GTTGATGCATTTGACTCCGC+TGG 0.558250 4_4:-11012272 None:intergenic
CTAGAGCAATGCATCAATCC+AGG 0.561417 4_4:+11013938 Msa0669350:CDS
GATGCATTTGACTCCGCTGG+TGG 0.562919 4_4:-11012269 None:intergenic
GACAAGAGGGTACACTTTGG+TGG 0.569591 4_4:+11011711 Msa0669350:CDS
ATTTAACCTAACTGGACAAG+AGG 0.571063 4_4:+11011697 Msa0669350:CDS
CAATGCATCAATCCAGGACC+AGG 0.571950 4_4:+11013944 Msa0669350:CDS
CGTGGAACTGGAACCCAATG+TGG 0.578205 4_4:-11013875 None:intergenic
GTATAATTAAATTCAAATGG+TGG 0.578940 4_4:-11014109 None:intergenic
ACCATTTCTGTGCCAGTGGG+AGG 0.579775 4_4:+11014382 Msa0669350:CDS
ACACTATAACACTGACGTTG+TGG 0.581153 4_4:+11013081 Msa0669350:CDS
TTGGGAAGATTAGCTGGTGG+AGG 0.584706 4_4:-11014638 None:intergenic
GATTTAGGTGCAAATGCAAG+TGG 0.589023 4_4:-11014166 None:intergenic
ATAACAGCCCATCCTCCCAC+TGG 0.590871 4_4:-11014394 None:intergenic
TACAAGCTCAAAGTGAAGCA+AGG 0.595483 4_4:+11012950 Msa0669350:CDS
CTGGACAAGAGGGTACACTT+TGG 0.595930 4_4:+11011708 Msa0669350:CDS
ATGTGTTCATCTACTTTACG+TGG 0.597501 4_4:-11013893 None:intergenic
GTGTAAAACTTGTGAGCTGT+TGG 0.601475 4_4:-11013301 None:intergenic
ATGCATTTGACTCCGCTGGT+GGG 0.601923 4_4:-11012268 None:intergenic
TAACACCATTTCTGTGCCAG+TGG 0.602566 4_4:+11014378 Msa0669350:CDS
CGGTTCCAACACTTGAAGAA+CGG 0.606020 4_4:-11013186 None:intergenic
ATAAACAACAACTCCTCTAG+TGG 0.606065 4_4:-11013218 None:intergenic
TAGGTATAGCTGCCACCAGA+TGG 0.606138 4_4:-11011671 None:intergenic
GGCACTGTTGTTCCAAGAGT+AGG 0.606785 4_4:-11014614 None:intergenic
CCAACACTTGAAGAACGGTA+TGG 0.608437 4_4:-11013181 None:intergenic
AAATAATGTACTCAACACTT+GGG 0.616786 4_4:-11014656 None:intergenic
ATTGAGAAACCTCTACCCCT+AGG 0.617029 4_4:-11014028 None:intergenic
TGGAAACACACTTGCCATGG+GGG 0.620681 4_4:+11014561 Msa0669350:CDS
TTTAACCTAACTGGACAAGA+GGG 0.625977 4_4:+11011698 Msa0669350:CDS
TCGCTCATACCCCTTCCCAA+AGG 0.634666 4_4:+11011799 Msa0669350:CDS
ACCGCTAACATAACCACTAG+AGG 0.642290 4_4:+11013205 Msa0669350:CDS
ACAAAATACAGCTATTCTTG+GGG 0.644652 4_4:+11014237 Msa0669350:CDS
AAACAATACCAAGCAATATG+GGG 0.644866 4_4:-11011835 None:intergenic
GATCAACATTAGCCAATGAA+GGG 0.645575 4_4:-11014135 None:intergenic
CGGCAGTGATAACCTGTGGA+CGG 0.647467 4_4:-11010204 None:intergenic
TTGGAAACACACTTGCCATG+GGG 0.647612 4_4:+11014560 Msa0669350:CDS
AACACCATTTCTGTGCCAGT+GGG 0.650163 4_4:+11014379 Msa0669350:CDS
TTGACGGCAGTGATAACCTG+TGG 0.667579 4_4:-11010208 None:intergenic
CCAACAATTAATGCTCGAGA+GGG 0.669858 4_4:+11010247 Msa0669350:CDS
CACTTGGGAAGATTAGCTGG+TGG 0.669877 4_4:-11014641 None:intergenic
CAATGCCCAATACCATCTGG+TGG 0.674666 4_4:+11011659 Msa0669350:CDS
GCTTAATGATAAGGGCACCA+TGG 0.676516 4_4:-11011766 None:intergenic
GGGTATGAGCGACCTAATCG+TGG 0.677346 4_4:-11011788 None:intergenic
TTTAGTACAACACAACGACC+TGG 0.677465 4_4:-11013962 None:intergenic
TATTCTGGACCATCTGACCA+AGG 0.704441 4_4:-11011629 None:intergenic
AGATACGGGTAACCCACCAG+CGG 0.756602 4_4:+11012256 Msa0669350:CDS
CACCATGGACAGTAGCACGT+AGG 0.762654 4_4:-11011751 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTTTTAAGTAAAATAA+AGG - chr4_4:11009537-11009556 None:intergenic 10.0%
!! ATATATAATAAAATAAGCAA+AGG + chr4_4:11011327-11011346 Msa0669350:intron 10.0%
!!! CTATTTTTTTTTTATTGAAA+GGG - chr4_4:11011508-11011527 None:intergenic 10.0%
!!! ACTATTTTTTTTTTATTGAA+AGG - chr4_4:11011509-11011528 None:intergenic 10.0%
!!! TTTTAAATGTAATTCTAATT+TGG + chr4_4:11011561-11011580 Msa0669350:intron 10.0%
!! AAATATGAATTCTTATAATT+TGG - chr4_4:11011897-11011916 None:intergenic 10.0%
!! ATATATAATAATTAAATTGC+AGG + chr4_4:11012188-11012207 Msa0669350:intron 10.0%
!! TATATAATAATTAAATTGCA+GGG + chr4_4:11012189-11012208 Msa0669350:intron 10.0%
!! TTCAATTAATTAACAAATTT+GGG + chr4_4:11012346-11012365 Msa0669350:intron 10.0%
!! TAAATTTCCATATATTCTAA+AGG - chr4_4:11009498-11009517 None:intergenic 15.0%
!! TATAACTAGATTGAAATAAA+AGG + chr4_4:11009747-11009766 Msa0669350:intron 15.0%
!! TGTTACTAAACTTTATAATA+TGG + chr4_4:11010105-11010124 Msa0669350:intron 15.0%
!!! TTAATTTTATTTTGATATCC+AGG + chr4_4:11010153-11010172 Msa0669350:intron 15.0%
!!! TTCTCTAATATTTTTATTGA+GGG - chr4_4:11010350-11010369 None:intergenic 15.0%
!!! TTTCTCTAATATTTTTATTG+AGG - chr4_4:11010351-11010370 None:intergenic 15.0%
!!! TTACATGATCATTATTTTAA+CGG + chr4_4:11010510-11010529 Msa0669350:intron 15.0%
!!! ATTTTTTACATAGTTGTTTT+TGG - chr4_4:11010879-11010898 None:intergenic 15.0%
!! AAAGAGAATATTCTTAAAAT+AGG - chr4_4:11010979-11010998 None:intergenic 15.0%
!!! AATCAATTTTATCATAAAAG+AGG + chr4_4:11011111-11011130 Msa0669350:intron 15.0%
!!! ATCAATTTTATCATAAAAGA+GGG + chr4_4:11011112-11011131 Msa0669350:intron 15.0%
!!! TTATTTTTCTCATATGTATA+GGG + chr4_4:11011530-11011549 Msa0669350:intron 15.0%
!! ATATAATAATTAAATTGCAG+GGG + chr4_4:11012190-11012209 Msa0669350:intron 15.0%
!! CTTCAATTAATTAACAAATT+TGG + chr4_4:11012345-11012364 Msa0669350:intron 15.0%
!! TCATATCTTAAAATATAGTT+TGG - chr4_4:11012795-11012814 None:intergenic 15.0%
!!! ATTTAATTTATATGCACTTT+CGG + chr4_4:11013416-11013435 Msa0669350:intron 15.0%
!! TTCAATGATCATAAATATAT+TGG + chr4_4:11013605-11013624 Msa0669350:intron 15.0%
!!! GAAATTTTGTATATATATAC+AGG + chr4_4:11013839-11013858 Msa0669350:intron 15.0%
!! TCAGTATAATTAAATTCAAA+TGG - chr4_4:11014115-11014134 None:intergenic 15.0%
!! AATTAACTCAAGAATTTATA+AGG - chr4_4:11014691-11014710 None:intergenic 15.0%
!! AACATAAAATGTGTCATATT+TGG - chr4_4:11009359-11009378 None:intergenic 20.0%
!!! ATTTCTTCCTTTAGAATATA+TGG + chr4_4:11009488-11009507 Msa0669350:intron 20.0%
!! CTAGTTATAAAGATTTACTA+TGG - chr4_4:11009736-11009755 None:intergenic 20.0%
!! AAGAAAAGATTAAAACAAGT+TGG - chr4_4:11009982-11010001 None:intergenic 20.0%
!!! TAATATTTTTATTGAGGGTA+AGG - chr4_4:11010345-11010364 None:intergenic 20.0%
!! GAAATAAATTAAACAAACCA+TGG + chr4_4:11010367-11010386 Msa0669350:intron 20.0%
!!! ATCAATTTTTTGACGAATTA+TGG - chr4_4:11011297-11011316 None:intergenic 20.0%
!! ATACTATTGTTAAAATGAGA+TGG - chr4_4:11011476-11011495 None:intergenic 20.0%
!!! GTTATTTTTCTCATATGTAT+AGG + chr4_4:11011529-11011548 Msa0669350:intron 20.0%
!!! GTTTTTTCACATTACATATT+TGG + chr4_4:11011854-11011873 Msa0669350:intron 20.0%
!!! TAAGAATTCATATTTTTCTG+TGG + chr4_4:11011902-11011921 Msa0669350:intron 20.0%
!!! TCTATTTTAGTAGATGAAAT+TGG + chr4_4:11012385-11012404 Msa0669350:intron 20.0%
!! TAAGAAATTTGGATAAAGAT+TGG - chr4_4:11012457-11012476 None:intergenic 20.0%
!! CATGTAAAAGTTAAGAAATT+TGG - chr4_4:11012468-11012487 None:intergenic 20.0%
!!! CTTTCTTTTACAATAAACTT+AGG + chr4_4:11012500-11012519 Msa0669350:intron 20.0%
!! AAATTCAATTATCTTTGGTA+AGG - chr4_4:11012728-11012747 None:intergenic 20.0%
!!! ATCTGAAATTCAATTATCTT+TGG - chr4_4:11012733-11012752 None:intergenic 20.0%
!!! TCATGATTGTTTTAACATTT+TGG + chr4_4:11012905-11012924 Msa0669350:intron 20.0%
!! TGTAATAAGAATCAATAACT+TGG - chr4_4:11013150-11013169 None:intergenic 20.0%
!!! AGTTATTGATTCTTATTACA+TGG + chr4_4:11013150-11013169 Msa0669350:CDS 20.0%
!! TTACAGGTATGATATAAATA+GGG + chr4_4:11013329-11013348 Msa0669350:intron 20.0%
!! TGTGTATATATAATCAACTT+TGG + chr4_4:11013386-11013405 Msa0669350:intron 20.0%
!! CACCTAATTATTATCAAATT+CGG + chr4_4:11013471-11013490 Msa0669350:intron 20.0%
!!! ATCCGAATTTGATAATAATT+AGG - chr4_4:11013476-11013495 None:intergenic 20.0%
!! TACTCTATAGTGAAAATATT+TGG + chr4_4:11013752-11013771 Msa0669350:intron 20.0%
!! TGGCATTAAAAATATCTAAA+TGG + chr4_4:11013772-11013791 Msa0669350:intron 20.0%
!! GGCATTAAAAATATCTAAAT+GGG + chr4_4:11013773-11013792 Msa0669350:intron 20.0%
!!! AATGGGTATATCTTTTTTTT+AGG + chr4_4:11013790-11013809 Msa0669350:intron 20.0%
!! GTATAATTAAATTCAAATGG+TGG - chr4_4:11014112-11014131 None:intergenic 20.0%
!!! ATTGACAAAAAGTGTAATAA+AGG + chr4_4:11014769-11014788 Msa0669350:three_prime_UTR 20.0%
!! CAATTTGATAAATGTTTACA+GGG - chr4_4:11014841-11014860 None:intergenic 20.0%
!! TCAATTTGATAAATGTTTAC+AGG - chr4_4:11014842-11014861 None:intergenic 20.0%
!! ATTTTATGTTTTCTCTAGCA+TGG + chr4_4:11009369-11009388 Msa0669350:CDS 25.0%
! TACTTTCAATGTATTGTTGA+TGG + chr4_4:11009445-11009464 Msa0669350:intron 25.0%
! ACTTTCAATGTATTGTTGAT+GGG + chr4_4:11009446-11009465 Msa0669350:intron 25.0%
! ATAAAGGAAGAAAGCTATAT+AGG - chr4_4:11009521-11009540 None:intergenic 25.0%
! TATGATCCACATGAAAAAAT+AGG + chr4_4:11009629-11009648 Msa0669350:intron 25.0%
! ATGATCCACATGAAAAAATA+GGG + chr4_4:11009630-11009649 Msa0669350:intron 25.0%
!! CAATCTATTTTCTTTTGCAA+AGG - chr4_4:11009907-11009926 None:intergenic 25.0%
! TCTAAAAAAGCTCAAACTTA+AGG - chr4_4:11010064-11010083 None:intergenic 25.0%
! TAATTCTTGAATTCAAACCA+TGG - chr4_4:11010387-11010406 None:intergenic 25.0%
! AAATACAAGAAACGTTTAAC+AGG - chr4_4:11010432-11010451 None:intergenic 25.0%
!!! TTTCTTTTCCATTTTTTCAG+AGG + chr4_4:11010752-11010771 Msa0669350:intron 25.0%
! TTTAAAAACATATGAGACTC+CGG + chr4_4:11011187-11011206 Msa0669350:intron 25.0%
! GGTTACATTATAAAATATGC+AGG + chr4_4:11011582-11011601 Msa0669350:intron 25.0%
!!! TCATTCAATTTTTAACTCCT+TGG + chr4_4:11011612-11011631 Msa0669350:CDS 25.0%
! AAAACAATACCAAGCAATAT+GGG - chr4_4:11011839-11011858 None:intergenic 25.0%
! AAAAACAATACCAAGCAATA+TGG - chr4_4:11011840-11011859 None:intergenic 25.0%
! CTATCAAACGTCAATAAATA+TGG + chr4_4:11012067-11012086 Msa0669350:intron 25.0%
! AGAAATGAATTCAAATGTCA+TGG - chr4_4:11012107-11012126 None:intergenic 25.0%
! TCAAATGCATCAACAATTAA+TGG + chr4_4:11012281-11012300 Msa0669350:CDS 25.0%
! AAAACAATCATGAGTCATAT+GGG - chr4_4:11012898-11012917 None:intergenic 25.0%
! TAAAACAATCATGAGTCATA+TGG - chr4_4:11012899-11012918 None:intergenic 25.0%
! TTATATCATACCTGTAACAT+TGG - chr4_4:11013326-11013345 None:intergenic 25.0%
! GTTACAGGTATGATATAAAT+AGG + chr4_4:11013328-11013347 Msa0669350:intron 25.0%
! CATTTACTAATTGCAAGATT+CGG + chr4_4:11013502-11013521 Msa0669350:intron 25.0%
! ATATATACAGGTTTAACAAC+TGG + chr4_4:11013851-11013870 Msa0669350:intron 25.0%
!! ATGTTCATAACTTTTGGTAT+TGG + chr4_4:11013914-11013933 Msa0669350:CDS 25.0%
! AAAAGATTCATTGTTCATGT+TGG - chr4_4:11014002-11014021 None:intergenic 25.0%
!! GCATTTTACAACAATATTAG+TGG + chr4_4:11014058-11014077 Msa0669350:CDS 25.0%
! TACAAAATACAGCTATTCTT+GGG + chr4_4:11014236-11014255 Msa0669350:CDS 25.0%
! AATCATCCTATTCATATTCA+TGG + chr4_4:11014265-11014284 Msa0669350:CDS 25.0%
! ATCATCCTATTCATATTCAT+GGG + chr4_4:11014266-11014285 Msa0669350:CDS 25.0%
! TTAAACCCATGAATATGAAT+AGG - chr4_4:11014274-11014293 None:intergenic 25.0%
! GGATTAACAAAGTTAAACTT+TGG - chr4_4:11014349-11014368 None:intergenic 25.0%
! TTCTACTTTAAACATTATGC+AGG + chr4_4:11014515-11014534 Msa0669350:intron 25.0%
! TTAAACATTATGCAGGTATT+TGG + chr4_4:11014522-11014541 Msa0669350:intron 25.0%
! AAATAATGTACTCAACACTT+GGG - chr4_4:11014659-11014678 None:intergenic 25.0%
! TAAATAATGTACTCAACACT+TGG - chr4_4:11014660-11014679 None:intergenic 25.0%
AGAAATGAGAGATGTAGAAA+AGG - chr4_4:11009474-11009493 None:intergenic 30.0%
TGTATAGTCCTTATGTAAGA+AGG + chr4_4:11009576-11009595 Msa0669350:intron 30.0%
!!! GTTTTCCCTATTTTTTCATG+TGG - chr4_4:11009638-11009657 None:intergenic 30.0%
AAAGCTCAAACTTAAGGTAA+TGG - chr4_4:11010058-11010077 None:intergenic 30.0%
CAATGAAGAGTAAGATTGTA+GGG - chr4_4:11010307-11010326 None:intergenic 30.0%
TCAACATTATACTACATGCA+TGG - chr4_4:11010474-11010493 None:intergenic 30.0%
! AGCAATATCTTCTTGTTTCT+AGG - chr4_4:11010693-11010712 None:intergenic 30.0%
TTAAAAACATATGAGACTCC+GGG + chr4_4:11011188-11011207 Msa0669350:intron 30.0%
! GCTAAATTTTCTTTCTTTCC+CGG - chr4_4:11011209-11011228 None:intergenic 30.0%
CATTATAAAATATGCAGGCA+TGG + chr4_4:11011587-11011606 Msa0669350:intron 30.0%
TAATCGTGGCTTAATGATAA+GGG - chr4_4:11011777-11011796 None:intergenic 30.0%
AAACAATACCAAGCAATATG+GGG - chr4_4:11011838-11011857 None:intergenic 30.0%
TCAACAATTAATGGCTTATC+TGG + chr4_4:11012290-11012309 Msa0669350:CDS 30.0%
AATTGAAGACTTACGATCTT+GGG - chr4_4:11012333-11012352 None:intergenic 30.0%
TAATTGAAGACTTACGATCT+TGG - chr4_4:11012334-11012353 None:intergenic 30.0%
TCTCCACTTATTCACTTAAA+GGG - chr4_4:11012597-11012616 None:intergenic 30.0%
TTCTCCACTTATTCACTTAA+AGG - chr4_4:11012598-11012617 None:intergenic 30.0%
AAGTGAATAAGTGGAGAATA+AGG + chr4_4:11012600-11012619 Msa0669350:intron 30.0%
AGTGAATAAGTGGAGAATAA+GGG + chr4_4:11012601-11012620 Msa0669350:intron 30.0%
TTTACATTGCAGTATGAAGA+GGG - chr4_4:11012637-11012656 None:intergenic 30.0%
! TTTTACATTGCAGTATGAAG+AGG - chr4_4:11012638-11012657 None:intergenic 30.0%
TTCAATTATCTTTGGTAAGG+AGG - chr4_4:11012725-11012744 None:intergenic 30.0%
!! ATTGATAATGCGTAGTAAGT+AGG - chr4_4:11012981-11013000 None:intergenic 30.0%
!! CATTATAGGTGCTTTTGTTT+GGG - chr4_4:11013254-11013273 None:intergenic 30.0%
! AAGTTTTACACCAATGTTAC+AGG + chr4_4:11013313-11013332 Msa0669350:CDS 30.0%
TGATATAAATAGGGTTTCAC+TGG + chr4_4:11013338-11013357 Msa0669350:intron 30.0%
! CTTTCGGTGTAAATTAACTT+TGG + chr4_4:11013432-11013451 Msa0669350:intron 30.0%
! GAACACATGTTCATAACTTT+TGG + chr4_4:11013908-11013927 Msa0669350:CDS 30.0%
AATTCAAATGGTGGTTGATT+TGG - chr4_4:11014103-11014122 None:intergenic 30.0%
TAATTATACTGACCCTTCAT+TGG + chr4_4:11014123-11014142 Msa0669350:CDS 30.0%
ATTTGCACCTAAATCAACTA+AGG + chr4_4:11014174-11014193 Msa0669350:CDS 30.0%
CTACAAAATACAGCTATTCT+TGG + chr4_4:11014235-11014254 Msa0669350:CDS 30.0%
ACAAAATACAGCTATTCTTG+GGG + chr4_4:11014237-11014256 Msa0669350:CDS 30.0%
!! TGGGTTTAACTTTCATGTTT+TGG + chr4_4:11014285-11014304 Msa0669350:CDS 30.0%
AGATTTCAAGCAAATAATCC+AGG + chr4_4:11014418-11014437 Msa0669350:CDS 30.0%
ACATGTAAATAAAACCAACC+TGG - chr4_4:11014439-11014458 None:intergenic 30.0%
! GAAAGAATAGGCAAACATTT+TGG - chr4_4:11014795-11014814 None:intergenic 30.0%
CACTATGATGTTGAAAGAAT+AGG - chr4_4:11014807-11014826 None:intergenic 30.0%
GAAGCTAATAGAAGAGCAAA+AGG - chr4_4:11009395-11009414 None:intergenic 35.0%
ACACTACACCTTCTTACATA+AGG - chr4_4:11009587-11009606 None:intergenic 35.0%
ATTAATTGTTGGTCCTGGTA+AGG - chr4_4:11010239-11010258 None:intergenic 35.0%
CCAATGAAGAGTAAGATTGT+AGG - chr4_4:11010308-11010327 None:intergenic 35.0%
CCTACAATCTTACTCTTCAT+TGG + chr4_4:11010305-11010324 Msa0669350:CDS 35.0%
TACAAGAAACGTTTAACAGG+AGG - chr4_4:11010429-11010448 None:intergenic 35.0%
! AGCAAGATCCTCTGAAAAAA+TGG - chr4_4:11010763-11010782 None:intergenic 35.0%
TCATAAAAGAGGGTGAAGTT+TGG + chr4_4:11011122-11011141 Msa0669350:intron 35.0%
!! TTTTTAACTCCTTGGTCAGA+TGG + chr4_4:11011620-11011639 Msa0669350:CDS 35.0%
ACCTACCAATTTAACCTAAC+TGG + chr4_4:11011689-11011708 Msa0669350:CDS 35.0%
TCCAGTTAGGTTAAATTGGT+AGG - chr4_4:11011693-11011712 None:intergenic 35.0%
CTTGTCCAGTTAGGTTAAAT+TGG - chr4_4:11011697-11011716 None:intergenic 35.0%
ATTTAACCTAACTGGACAAG+AGG + chr4_4:11011697-11011716 Msa0669350:CDS 35.0%
TTTAACCTAACTGGACAAGA+GGG + chr4_4:11011698-11011717 Msa0669350:CDS 35.0%
CTAATCGTGGCTTAATGATA+AGG - chr4_4:11011778-11011797 None:intergenic 35.0%
CTTATCATTAAGCCACGATT+AGG + chr4_4:11011776-11011795 Msa0669350:CDS 35.0%
! TCTTGATAAACCTTTGGGAA+GGG - chr4_4:11011812-11011831 None:intergenic 35.0%
! TTCTTGATAAACCTTTGGGA+AGG - chr4_4:11011813-11011832 None:intergenic 35.0%
GGACTTCTTGATAAACCTTT+GGG - chr4_4:11011817-11011836 None:intergenic 35.0%
ATAATTAAATTGCAGGGGAG+TGG + chr4_4:11012195-11012214 Msa0669350:intron 35.0%
TGAGAGAAATGCAACAGATA+CGG + chr4_4:11012241-11012260 Msa0669350:CDS 35.0%
TTCCCCTTTAAGTGAATAAG+TGG + chr4_4:11012591-11012610 Msa0669350:intron 35.0%
CTCCACTTATTCACTTAAAG+GGG - chr4_4:11012596-11012615 None:intergenic 35.0%
ATTATCTTTGGTAAGGAGGT+TGG - chr4_4:11012721-11012740 None:intergenic 35.0%
TAGCTACAACTGTGAAACTA+TGG - chr4_4:11013042-11013061 None:intergenic 35.0%
TAGTTTCACAGTTGTAGCTA+TGG + chr4_4:11013042-11013061 Msa0669350:CDS 35.0%
!! AGTTTTCAAGAGAACGTCAA+CGG - chr4_4:11013125-11013144 None:intergenic 35.0%
ATAAACAACAACTCCTCTAG+TGG - chr4_4:11013221-11013240 None:intergenic 35.0%
!! AGAGGAGTTGTTGTTTATGA+TGG + chr4_4:11013223-11013242 Msa0669350:CDS 35.0%
! GCATTATAGGTGCTTTTGTT+TGG - chr4_4:11013255-11013274 None:intergenic 35.0%
TCAGGCATAATTGGCATTAT+AGG - chr4_4:11013268-11013287 None:intergenic 35.0%
TCATGGTTATCAGGCATAAT+TGG - chr4_4:11013277-11013296 None:intergenic 35.0%
TTTAACAACTGGTCCACATT+GGG + chr4_4:11013862-11013881 Msa0669350:CDS 35.0%
ATGTGTTCATCTACTTTACG+TGG - chr4_4:11013896-11013915 None:intergenic 35.0%
! ATGAATCTTTTGCTCTTCCT+AGG + chr4_4:11014011-11014030 Msa0669350:CDS 35.0%
! TGAATCTTTTGCTCTTCCTA+GGG + chr4_4:11014012-11014031 Msa0669350:CDS 35.0%
GATCAACATTAGCCAATGAA+GGG - chr4_4:11014138-11014157 None:intergenic 35.0%
! GTCTTCACCTTAGTTGATTT+AGG - chr4_4:11014184-11014203 None:intergenic 35.0%
!!! AACTTTCATGTTTTGGCACA+AGG + chr4_4:11014292-11014311 Msa0669350:CDS 35.0%
TTCAAGCAAATAATCCAGGT+TGG + chr4_4:11014422-11014441 Msa0669350:intron 35.0%
!! TTGGCTTGTACATTGTCATT+TGG + chr4_4:11014541-11014560 Msa0669350:CDS 35.0%
GAATAGTGAAGTCTTCAACC+TGG - chr4_4:11010174-11010193 None:intergenic 40.0%
ACCAACAATTAATGCTCGAG+AGG + chr4_4:11010246-11010265 Msa0669350:CDS 40.0%
CCAACAATTAATGCTCGAGA+GGG + chr4_4:11010247-11010266 Msa0669350:CDS 40.0%
TCTTGCTAACGAGTGTTCTA+AGG + chr4_4:11010776-11010795 Msa0669350:intron 40.0%
TCTGATAGTTGATCGACCAT+CGG + chr4_4:11010939-11010958 Msa0669350:intron 40.0%
GGAGAAAGAATTAAGTCCGA+TGG - chr4_4:11010958-11010977 None:intergenic 40.0%
! CTTGATAAACCTTTGGGAAG+GGG - chr4_4:11011811-11011830 None:intergenic 40.0%
GGGACTTCTTGATAAACCTT+TGG - chr4_4:11011818-11011837 None:intergenic 40.0%
TTGCATGCATTCACGCAATT+AGG + chr4_4:11011932-11011951 Msa0669350:intron 40.0%
GAGAGAAATGCAACAGATAC+GGG + chr4_4:11012242-11012261 Msa0669350:CDS 40.0%
AGGTTGGATTATGAAGAGGT+TGG - chr4_4:11012705-11012724 None:intergenic 40.0%
AAGGAGGTTGGATTATGAAG+AGG - chr4_4:11012709-11012728 None:intergenic 40.0%
TACAAGCTCAAAGTGAAGCA+AGG + chr4_4:11012950-11012969 Msa0669350:CDS 40.0%
ACACTATAACACTGACGTTG+TGG + chr4_4:11013081-11013100 Msa0669350:CDS 40.0%
TCCTCTAGTGGTTATGTTAG+CGG - chr4_4:11013209-11013228 None:intergenic 40.0%
!! TATAGGTGCTTTTGTTTGGG+AGG - chr4_4:11013251-11013270 None:intergenic 40.0%
GTTGGTGTATCATGGTTATC+AGG - chr4_4:11013286-11013305 None:intergenic 40.0%
GTGTAAAACTTGTGAGCTGT+TGG - chr4_4:11013304-11013323 None:intergenic 40.0%
GTTTAACAACTGGTCCACAT+TGG + chr4_4:11013861-11013880 Msa0669350:CDS 40.0%
TCATCTACTTTACGTGGAAC+TGG - chr4_4:11013890-11013909 None:intergenic 40.0%
TTTAGTACAACACAACGACC+TGG - chr4_4:11013965-11013984 None:intergenic 40.0%
! GAATCTTTTGCTCTTCCTAG+GGG + chr4_4:11014013-11014032 Msa0669350:CDS 40.0%
GGATCAACATTAGCCAATGA+AGG - chr4_4:11014139-11014158 None:intergenic 40.0%
GATTTAGGTGCAAATGCAAG+TGG - chr4_4:11014169-11014188 None:intergenic 40.0%
!!! CATGTTTTGGCACAAGGTTT+TGG + chr4_4:11014298-11014317 Msa0669350:CDS 40.0%
!! GAAATGGTGTTACGCATTTG+AGG - chr4_4:11014370-11014389 None:intergenic 40.0%
ATTTGGAAACACACTTGCCA+TGG + chr4_4:11014558-11014577 Msa0669350:CDS 40.0%
TTTGGAAACACACTTGCCAT+GGG + chr4_4:11014559-11014578 Msa0669350:CDS 40.0%
!!! ATGGCTTTTGAGGTTGAGAA+TGG + chr4_4:11014590-11014609 Msa0669350:CDS 40.0%
!!! TGGCTTTTGAGGTTGAGAAT+GGG + chr4_4:11014591-11014610 Msa0669350:CDS 40.0%
ATTTGCTTCTGCTGCTATCG+TGG + chr4_4:11009418-11009437 Msa0669350:CDS 45.0%
TCCTGGTAAGGTTCCATTGA+CGG - chr4_4:11010227-11010246 None:intergenic 45.0%
CGAGCATTAATTGTTGGTCC+TGG - chr4_4:11010244-11010263 None:intergenic 45.0%
CCCTCTCGAGCATTAATTGT+TGG - chr4_4:11010250-11010269 None:intergenic 45.0%
TATTCTGGACCATCTGACCA+AGG - chr4_4:11011632-11011651 None:intergenic 45.0%
!! GGGCATTGGGTTACATATTC+TGG - chr4_4:11011647-11011666 None:intergenic 45.0%
! ACCAGATGGTATTGGGCATT+GGG - chr4_4:11011660-11011679 None:intergenic 45.0%
!! GCTTAATGATAAGGGCACCA+TGG - chr4_4:11011769-11011788 None:intergenic 45.0%
CAAGAAGTCCCCATATTGCT+TGG + chr4_4:11011827-11011846 Msa0669350:CDS 45.0%
ATTAAATTGCAGGGGAGTGG+TGG + chr4_4:11012198-11012217 Msa0669350:intron 45.0%
ACATTGCAGTATGAAGAGGG+TGG - chr4_4:11012634-11012653 None:intergenic 45.0%
TCAAGAGAACGTCAACGGTT+TGG - chr4_4:11013120-11013139 None:intergenic 45.0%
CCAACACTTGAAGAACGGTA+TGG - chr4_4:11013184-11013203 None:intergenic 45.0%
CCATACCGTTCTTCAAGTGT+TGG + chr4_4:11013181-11013200 Msa0669350:CDS 45.0%
CGGTTCCAACACTTGAAGAA+CGG - chr4_4:11013189-11013208 None:intergenic 45.0%
ACCGCTAACATAACCACTAG+AGG + chr4_4:11013205-11013224 Msa0669350:CDS 45.0%
TGTGAGCTGTTGGTGTATCA+TGG - chr4_4:11013294-11013313 None:intergenic 45.0%
CTAGAGCAATGCATCAATCC+AGG + chr4_4:11013938-11013957 Msa0669350:CDS 45.0%
ATTGAGAAACCTCTACCCCT+AGG - chr4_4:11014031-11014050 None:intergenic 45.0%
GGGTAGAGGTTTCTCAATGT+TGG + chr4_4:11014033-11014052 Msa0669350:CDS 45.0%
GCAAATGCAAGTGGCTTAGA+AGG - chr4_4:11014160-11014179 None:intergenic 45.0%
TAACACCATTTCTGTGCCAG+TGG + chr4_4:11014378-11014397 Msa0669350:CDS 45.0%
AACACCATTTCTGTGCCAGT+GGG + chr4_4:11014379-11014398 Msa0669350:CDS 45.0%
TTGGAAACACACTTGCCATG+GGG + chr4_4:11014560-11014579 Msa0669350:CDS 45.0%
CAACACTTGGGAAGATTAGC+TGG - chr4_4:11014647-11014666 None:intergenic 45.0%
!! TTATTTAATAATTTATGAAT+TGG - chr4_4:11010819-11010838 None:intergenic 5.0%
!! AAACAAAATTATAAATAATA+TGG + chr4_4:11011051-11011070 Msa0669350:intron 5.0%
!!! AATTATTTAGAATAATTTTT+TGG - chr4_4:11012161-11012180 None:intergenic 5.0%
TTGACGGCAGTGATAACCTG+TGG - chr4_4:11010211-11010230 None:intergenic 50.0%
CAGGTTATCACTGCCGTCAA+TGG + chr4_4:11010211-11010230 Msa0669350:CDS 50.0%
ACCCAATGCCCAATACCATC+TGG + chr4_4:11011656-11011675 Msa0669350:CDS 50.0%
! CACCAGATGGTATTGGGCAT+TGG - chr4_4:11011661-11011680 None:intergenic 50.0%
CAATGCCCAATACCATCTGG+TGG + chr4_4:11011659-11011678 Msa0669350:CDS 50.0%
AGCTGCCACCAGATGGTATT+GGG - chr4_4:11011667-11011686 None:intergenic 50.0%
TAGCTGCCACCAGATGGTAT+TGG - chr4_4:11011668-11011687 None:intergenic 50.0%
TAGGTATAGCTGCCACCAGA+TGG - chr4_4:11011674-11011693 None:intergenic 50.0%
GTGTACCCTCTTGTCCAGTT+AGG - chr4_4:11011706-11011725 None:intergenic 50.0%
! CTGGACAAGAGGGTACACTT+TGG + chr4_4:11011708-11011727 Msa0669350:CDS 50.0%
! GACAAGAGGGTACACTTTGG+TGG + chr4_4:11011711-11011730 Msa0669350:CDS 50.0%
! TTCCTACGTGCTACTGTCCA+TGG + chr4_4:11011749-11011768 Msa0669350:CDS 50.0%
ATGCATTTGACTCCGCTGGT+GGG - chr4_4:11012271-11012290 None:intergenic 50.0%
GTTGATGCATTTGACTCCGC+TGG - chr4_4:11012275-11012294 None:intergenic 50.0%
GTGCGTGTGTGTCAGATTCA+TGG - chr4_4:11013642-11013661 None:intergenic 50.0%
CAATGCATCAATCCAGGACC+AGG + chr4_4:11013944-11013963 Msa0669350:CDS 50.0%
TTTGCTCTTCCTAGGGGTAG+AGG + chr4_4:11014019-11014038 Msa0669350:CDS 50.0%
TGGAAACACACTTGCCATGG+GGG + chr4_4:11014561-11014580 Msa0669350:CDS 50.0%
AAAGCCATTGCTAACCCCCA+TGG - chr4_4:11014578-11014597 None:intergenic 50.0%
!! GGGGTTAGCAATGGCTTTTG+AGG + chr4_4:11014580-11014599 Msa0669350:CDS 50.0%
GTTGAGAATGGGCCTACTCT+TGG + chr4_4:11014602-11014621 Msa0669350:CDS 50.0%
GGCACTGTTGTTCCAAGAGT+AGG - chr4_4:11014617-11014636 None:intergenic 50.0%
! TTGGGAAGATTAGCTGGTGG+AGG - chr4_4:11014641-11014660 None:intergenic 50.0%
! CACTTGGGAAGATTAGCTGG+TGG - chr4_4:11014644-11014663 None:intergenic 50.0%
CGGCAGTGATAACCTGTGGA+CGG - chr4_4:11010207-11010226 None:intergenic 55.0%
GCCGTCAATGGAACCTTACC+AGG + chr4_4:11010223-11010242 Msa0669350:CDS 55.0%
CACCATGGACAGTAGCACGT+AGG - chr4_4:11011754-11011773 None:intergenic 55.0%
GGGTATGAGCGACCTAATCG+TGG - chr4_4:11011791-11011810 None:intergenic 55.0%
TCGCTCATACCCCTTCCCAA+AGG + chr4_4:11011799-11011818 Msa0669350:CDS 55.0%
AGATACGGGTAACCCACCAG+CGG + chr4_4:11012256-11012275 Msa0669350:CDS 55.0%
GATGCATTTGACTCCGCTGG+TGG - chr4_4:11012272-11012291 None:intergenic 55.0%
! AGAACGTCAACGGTTTGGCC+AGG - chr4_4:11013115-11013134 None:intergenic 55.0%
CGTGGAACTGGAACCCAATG+TGG - chr4_4:11013878-11013897 None:intergenic 55.0%
ACAACACAACGACCTGGTCC+TGG - chr4_4:11013959-11013978 None:intergenic 55.0%
ACCATTTCTGTGCCAGTGGG+AGG + chr4_4:11014382-11014401 Msa0669350:CDS 55.0%
TCCTCCCACTGGCACAGAAA+TGG - chr4_4:11014386-11014405 None:intergenic 55.0%
TTTCTGTGCCAGTGGGAGGA+TGG + chr4_4:11014386-11014405 Msa0669350:CDS 55.0%
TTCTGTGCCAGTGGGAGGAT+GGG + chr4_4:11014387-11014406 Msa0669350:CDS 55.0%
ATAACAGCCCATCCTCCCAC+TGG - chr4_4:11014397-11014416 None:intergenic 55.0%
! CTTGCCATGGGGGTTAGCAA+TGG + chr4_4:11014571-11014590 Msa0669350:CDS 55.0%
! GGAAGATTAGCTGGTGGAGG+AGG - chr4_4:11014638-11014657 None:intergenic 55.0%
TCAGCGATTGTGCCGTCCAC+AGG + chr4_4:11010192-11010211 Msa0669350:CDS 60.0%
GACGTTGTGGTGCTTGCGCC+TGG + chr4_4:11013094-11013113 Msa0669350:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 11009343 11014877 11009343 ID=Msa0669350;Name=Msa0669350
chr4_4 mRNA 11009343 11014877 11009343 ID=Msa0669350-mRNA-1;Parent=Msa0669350;Name=Msa0669350-mRNA-1;_AED=0.09;_eAED=0.09;_QI=19|1|1|1|1|1|7|210|572
chr4_4 exon 11009343 11009454 11009343 ID=Msa0669350-mRNA-1:exon:3282;Parent=Msa0669350-mRNA-1
chr4_4 exon 11010175 11010326 11010175 ID=Msa0669350-mRNA-1:exon:3283;Parent=Msa0669350-mRNA-1
chr4_4 exon 11011604 11011848 11011604 ID=Msa0669350-mRNA-1:exon:3284;Parent=Msa0669350-mRNA-1
chr4_4 exon 11012210 11012338 11012210 ID=Msa0669350-mRNA-1:exon:3285;Parent=Msa0669350-mRNA-1
chr4_4 exon 11012945 11013334 11012945 ID=Msa0669350-mRNA-1:exon:3286;Parent=Msa0669350-mRNA-1
chr4_4 exon 11013861 11014439 11013861 ID=Msa0669350-mRNA-1:exon:3287;Parent=Msa0669350-mRNA-1
chr4_4 exon 11014537 11014877 11014537 ID=Msa0669350-mRNA-1:exon:3288;Parent=Msa0669350-mRNA-1
chr4_4 five_prime_UTR 11009343 11009361 11009343 ID=Msa0669350-mRNA-1:five_prime_utr;Parent=Msa0669350-mRNA-1
chr4_4 CDS 11009362 11009454 11009362 ID=Msa0669350-mRNA-1:cds;Parent=Msa0669350-mRNA-1
chr4_4 CDS 11010175 11010326 11010175 ID=Msa0669350-mRNA-1:cds;Parent=Msa0669350-mRNA-1
chr4_4 CDS 11011604 11011848 11011604 ID=Msa0669350-mRNA-1:cds;Parent=Msa0669350-mRNA-1
chr4_4 CDS 11012210 11012338 11012210 ID=Msa0669350-mRNA-1:cds;Parent=Msa0669350-mRNA-1
chr4_4 CDS 11012945 11013334 11012945 ID=Msa0669350-mRNA-1:cds;Parent=Msa0669350-mRNA-1
chr4_4 CDS 11013861 11014439 11013861 ID=Msa0669350-mRNA-1:cds;Parent=Msa0669350-mRNA-1
chr4_4 CDS 11014537 11014667 11014537 ID=Msa0669350-mRNA-1:cds;Parent=Msa0669350-mRNA-1
chr4_4 three_prime_UTR 11014668 11014877 11014668 ID=Msa0669350-mRNA-1:three_prime_utr;Parent=Msa0669350-mRNA-1
Gene Sequence

>Msa0669350

ATGACACATTTTATGTTTTCTCTAGCATGGCCTTTTGCTCTTCTATTAGCTTCTTCATTTGCTTCTGCTGCTATCGTGGAACATACTTTCAATGTTGAAGACTTCACTATTCAGCGATTGTGCCGTCCACAGGTTATCACTGCCGTCAATGGAACCTTACCAGGACCAACAATTAATGCTCGAGAGGGTGACACTGTTATCATTCATGCATTCAACAAATCACCCTACAATCTTACTCTTCATTGGCATGGAATCATTCAATTTTTAACTCCTTGGTCAGATGGTCCAGAATATGTAACCCAATGCCCAATACCATCTGGTGGCAGCTATACCTACCAATTTAACCTAACTGGACAAGAGGGTACACTTTGGTGGCATGCACACTCTTCTTTCCTACGTGCTACTGTCCATGGTGCCCTTATCATTAAGCCACGATTAGGTCGCTCATACCCCTTCCCAAAGGTTTATCAAGAAGTCCCCATATTGCTTGGGGAGTGGTGGAATGCTAATGTTGAAGAAGTTGAGAGAAATGCAACAGATACGGGTAACCCACCAGCGGAGTCAAATGCATCAACAATTAATGGCTTATCTGGTGACTTGTATAACTGCTCCCAAGATCAGAATTACAAGCTCAAAGTGAAGCAAGGAAAAACCTACTTACTACGCATTATCAATGCTGCACTCAATGAACAACATTTCTTCAAGATAGCAAACCATAGTTTCACAGTTGTAGCTATGGATGCTATCTACACAGAACACTATAACACTGACGTTGTGGTGCTTGCGCCTGGCCAAACCGTTGACGTTCTCTTGAAAACTAACCAAGTTATTGATTCTTATTACATGGTTATGACACCATACCGTTCTTCAAGTGTTGGAACCGCTAACATAACCACTAGAGGAGTTGTTGTTTATGATGGTGCCTCCCAAACAAAAGCACCTATAATGCCAATTATGCCTGATAACCATGATACACCAACAGCTCACAAGTTTTACACCAATGTTACAGGTTTAACAACTGGTCCACATTGGGTTCCAGTTCCACGTAAAGTAGATGAACACATGTTCATAACTTTTGGTATTGGTCTAGAGCAATGCATCAATCCAGGACCAGGTCGTTGTGTTGTACTAAACTCTCGACTTTCTGCCAACATGAACAATGAATCTTTTGCTCTTCCTAGGGGTAGAGGTTTCTCAATGTTGGAAGCATTTTACAACAATATTAGTGGTGTGTACACAAAAGATTTTCCAAATCAACCACCATTTGAATTTAATTATACTGACCCTTCATTGGCTAATGTTGATCCTTCTAAGCCACTTGCATTTGCACCTAAATCAACTAAGGTGAAGACTTTGAAGTTTAATTCAACGATTGAGATTGTGCTACAAAATACAGCTATTCTTGGGGCAGAGAATCATCCTATTCATATTCATGGGTTTAACTTTCATGTTTTGGCACAAGGTTTTGGAAATTATAATGCCACTAGAGATGAACCAAAGTTTAACTTTGTTAATCCTCAAATGCGTAACACCATTTCTGTGCCAGTGGGAGGATGGGCTGTTATTAGATTTCAAGCAAATAATCCAGGTATTTGGCTTGTACATTGTCATTTGGAAACACACTTGCCATGGGGGTTAGCAATGGCTTTTGAGGTTGAGAATGGGCCTACTCTTGGAACAACAGTGCCTCCTCCACCAGCTAATCTTCCCAAGTGTTGA
Protein sequence

>Msa0669350

MTHFMFSLAWPFALLLASSFASAAIVEHTFNVEDFTIQRLCRPQVITAVNGTLPGPTINAREGDTVIIHAFNKSPYNLTLHWHGIIQFLTPWSDGPEYVTQCPIPSGGSYTYQFNLTGQEGTLWWHAHSSFLRATVHGALIIKPRLGRSYPFPKVYQEVPILLGEWWNANVEEVERNATDTGNPPAESNASTINGLSGDLYNCSQDQNYKLKVKQGKTYLLRIINAALNEQHFFKIANHSFTVVAMDAIYTEHYNTDVVVLAPGQTVDVLLKTNQVIDSYYMVMTPYRSSSVGTANITTRGVVVYDGASQTKAPIMPIMPDNHDTPTAHKFYTNVTGLTTGPHWVPVPRKVDEHMFITFGIGLEQCINPGPGRCVVLNSRLSANMNNESFALPRGRGFSMLEAFYNNISGVYTKDFPNQPPFEFNYTDPSLANVDPSKPLAFAPKSTKVKTLKFNSTIEIVLQNTAILGAENHPIHIHGFNFHVLAQGFGNYNATRDEPKFNFVNPQMRNTISVPVGGWAVIRFQANNPGIWLVHCHLETHLPWGLAMAFEVENGPTLGTTVPPPPANLPKC*