AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0534610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0534610 MtrunA17_Chr4g0008521 95.629 572 25 0 1 572 1 572 0.0 1123
Msa0534610 MtrunA17_Chr7g0240971 62.609 575 207 5 1 572 1 570 0.0 716
Msa0534610 MtrunA17_Chr7g0240991 57.719 570 227 9 10 572 9 571 0.0 684
Msa0534610 MtrunA17_Chr5g0404361 45.760 566 288 5 8 572 64 611 0.0 535
Msa0534610 MtrunA17_Chr5g0437681 47.627 569 275 9 14 572 12 567 0.0 532
Msa0534610 MtrunA17_Chr3g0114211 48.330 569 273 9 12 572 15 570 0.0 525
Msa0534610 MtrunA17_Chr5g0431581 47.153 562 281 8 14 572 12 560 0.0 524
Msa0534610 MtrunA17_Chr5g0404341 44.326 564 295 6 12 572 19 566 1.13e-175 508
Msa0534610 MtrunA17_Chr5g0429511 44.565 552 291 4 21 572 22 558 5.68e-173 501
Msa0534610 MtrunA17_Chr4g0005921 45.263 570 293 5 8 572 2 557 2.00e-172 500
Msa0534610 MtrunA17_Chr3g0104061 44.283 551 291 4 22 572 22 556 3.37e-172 499
Msa0534610 MtrunA17_Chr7g0238981 45.276 561 277 7 27 572 48 593 5.28e-172 500
Msa0534610 MtrunA17_Chr7g0238941 45.234 577 295 6 4 572 15 578 6.54e-171 497
Msa0534610 MtrunA17_Chr7g0237491 45.299 585 289 10 7 572 8 580 4.98e-168 489
Msa0534610 MtrunA17_Chr5g0436461 43.881 572 292 7 4 572 9 554 8.31e-166 483
Msa0534610 MtrunA17_Chr7g0237441 44.696 575 286 9 15 572 21 580 4.25e-165 482
Msa0534610 MtrunA17_Chr4g0031901 45.191 551 281 8 13 555 31 568 1.72e-164 481
Msa0534610 MtrunA17_Chr7g0238351 44.779 565 289 7 21 572 27 581 2.49e-164 480
Msa0534610 MtrunA17_Chr2g0278811 40.464 603 310 9 5 572 19 607 2.00e-160 471
Msa0534610 MtrunA17_Chr8g0346581 40.693 548 310 8 28 572 36 571 1.60e-149 442
Msa0534610 MtrunA17_Chr3g0133861 41.228 342 182 11 228 562 1 330 6.15e-75 241
Msa0534610 MtrunA17_Chr3g0133871 47.959 196 101 1 12 206 17 212 2.44e-61 202
Msa0534610 MtrunA17_Chr8g0382691 30.404 569 312 20 21 551 29 551 1.19e-60 211
Msa0534610 MtrunA17_Chr3g0118321 28.720 578 351 17 1 550 1 545 1.18e-54 194
Msa0534610 MtrunA17_Chr3g0118331 28.265 559 334 17 23 550 22 544 6.23e-48 176
Msa0534610 MtrunA17_Chr5g0436431 37.190 242 131 5 292 530 5 228 3.23e-44 157
Msa0534610 MtrunA17_Chr4g0056511 26.620 571 371 13 14 572 15 549 2.80e-40 154
Msa0534610 MtrunA17_Chr3g0079321 25.473 581 394 11 1 572 1 551 1.99e-39 152
Msa0534610 MtrunA17_Chr4g0038671 25.599 543 338 18 11 538 7 498 3.96e-31 127
Msa0534610 MtrunA17_Chr3g0143671 25.235 531 340 14 49 572 53 533 8.04e-31 126
Msa0534610 MtrunA17_Chr8g0374261 25.681 514 318 16 29 532 32 491 1.35e-30 125
Msa0534610 MtrunA17_Chr8g0374271 25.532 517 315 15 29 532 34 493 2.81e-30 125
Msa0534610 MtrunA17_Chr3g0121161 24.638 552 370 16 8 554 48 558 4.90e-29 121
Msa0534610 MtrunA17_Chr3g0121171 24.319 551 373 15 8 554 1158 1668 1.02e-28 122
Msa0534610 MtrunA17_Chr3g0121171 24.457 552 371 16 8 554 583 1093 1.30e-28 122
Msa0534610 MtrunA17_Chr3g0121171 24.304 539 364 15 20 554 20 518 1.73e-27 118
Msa0534610 MtrunA17_Chr3g0121171 23.732 552 366 17 8 554 1733 2234 3.91e-23 104
Msa0534610 MtrunA17_Chr4g0024861 24.457 552 360 17 28 572 29 530 1.20e-28 120
Msa0534610 MtrunA17_Chr5g0402831 25.725 552 349 16 28 572 33 530 1.34e-28 119
Msa0534610 MtrunA17_Chr2g0322441 25.000 552 357 17 28 572 32 533 1.70e-28 119
Msa0534610 MtrunA17_Chr3g0121151 24.549 554 368 17 8 554 48 558 1.83e-28 119
Msa0534610 MtrunA17_Chr1g0157341 23.585 530 350 14 49 572 52 532 8.15e-27 114
Msa0534610 MtrunA17_Chr7g0234061 25.189 528 341 18 19 532 22 509 8.16e-27 115
Msa0534610 MtrunA17_Chr4g0043261 23.133 549 374 14 8 552 7 511 1.13e-26 114
Msa0534610 MtrunA17_Chr4g0043251 24.612 516 341 16 43 552 44 517 8.87e-24 105
Msa0534610 MtrunA17_Chr3g0118341 36.000 150 92 4 28 175 28 175 5.78e-22 94.4
Msa0534610 MtrunA17_Chr4g0075071 24.848 495 307 13 49 532 54 494 2.24e-21 97.8
Msa0534610 MtrunA17_Chr3g0118351 28.135 327 206 11 238 550 5 316 8.31e-20 91.3
Msa0534610 MtrunA17_Chr4g0024641 24.000 450 286 17 92 535 17 416 5.24e-15 78.6
Msa0534610 MtrunA17_Chr1g0197311 28.641 206 111 6 44 217 42 243 6.78e-13 68.9
Msa0534610 MtrunA17_Chr3g0118931 39.130 69 42 0 482 550 10 78 3.54e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0534610 AT3G09220.1 58.047 553 217 7 8 555 8 550 0.0 670
Msa0534610 AT5G01050.1 54.690 565 242 5 12 572 14 568 0.0 648
Msa0534610 AT5G01040.1 53.217 575 254 8 1 572 4 566 0.0 639
Msa0534610 AT3G09220.2 57.792 462 180 6 99 555 2 453 0.0 553
Msa0534610 AT5G05390.1 47.544 570 285 6 6 572 7 565 0.0 546
Msa0534610 AT2G40370.1 46.794 577 279 9 13 572 15 580 0.0 526
Msa0534610 AT5G03260.1 46.491 570 287 5 4 572 5 557 3.34e-180 520
Msa0534610 AT2G30210.1 46.194 578 286 8 6 572 7 570 3.21e-179 518
Msa0534610 AT5G58910.2 46.561 567 283 6 12 572 15 567 5.03e-176 510
Msa0534610 AT5G58910.3 46.737 567 281 7 12 572 15 566 4.19e-175 507
Msa0534610 AT2G38080.1 44.406 572 300 6 1 572 5 558 6.64e-175 506
Msa0534610 AT5G07130.1 46.701 576 281 11 11 572 6 569 1.10e-173 504
Msa0534610 AT2G29130.1 45.804 572 294 6 8 572 11 573 4.69e-173 502
Msa0534610 AT5G01190.1 45.374 562 292 4 12 572 11 558 1.59e-170 495
Msa0534610 AT5G58910.1 46.753 539 261 6 39 572 6 523 7.75e-169 490
Msa0534610 AT5G60020.1 44.097 576 296 6 13 572 12 577 2.70e-167 488
Msa0534610 AT5G60020.3 45.290 552 276 6 37 572 4 545 1.76e-166 484
Msa0534610 AT5G09360.1 42.984 563 300 9 14 572 24 569 1.42e-165 483
Msa0534610 AT5G01190.2 44.840 562 281 5 12 572 11 544 2.14e-164 479
Msa0534610 AT1G18140.1 43.137 561 289 9 30 572 33 581 3.85e-156 459
Msa0534610 AT2G46570.1 42.626 556 298 9 23 572 29 569 3.38e-153 451
Msa0534610 AT5G60020.2 43.446 534 276 6 13 530 12 535 4.44e-148 437
Msa0534610 AT5G07130.2 47.059 493 238 10 91 572 4 484 1.50e-146 431
Msa0534610 AT5G07130.3 47.059 493 238 10 91 572 4 484 1.50e-146 431
Msa0534610 AT5G48100.1 40.429 559 300 12 12 555 6 546 8.05e-143 425
Msa0534610 AT2G46570.2 42.485 499 266 9 80 572 1 484 7.25e-133 396
Msa0534610 AT5G21100.1 29.217 575 343 16 21 567 19 557 4.48e-62 214
Msa0534610 AT4G39830.1 29.088 581 328 19 13 551 23 561 2.62e-60 210
Msa0534610 AT5G21105.1 27.768 569 353 13 10 550 20 558 9.73e-58 203
Msa0534610 AT5G21105.3 27.140 549 342 13 10 530 20 538 9.50e-49 178
Msa0534610 AT4G39830.2 28.543 508 280 18 85 551 1 466 4.67e-45 166
Msa0534610 AT4G39830.3 28.628 503 276 18 90 551 4 464 1.59e-44 165
Msa0534610 AT4G39830.4 28.008 507 271 17 85 551 1 453 9.55e-42 157
Msa0534610 AT4G25240.2 26.876 573 370 16 12 572 15 550 1.07e-40 156
Msa0534610 AT4G25240.1 26.876 573 370 16 12 572 15 550 1.07e-40 156
Msa0534610 AT5G51480.1 25.569 571 377 14 14 572 16 550 3.55e-40 154
Msa0534610 AT4G12420.1 24.764 529 358 14 12 532 11 507 1.15e-34 139
Msa0534610 AT4G12420.2 24.764 529 358 14 12 532 11 507 1.15e-34 139
Msa0534610 AT1G41830.1 25.391 575 358 17 14 572 12 531 1.79e-33 134
Msa0534610 AT1G76160.1 25.130 577 371 18 7 572 4 530 5.60e-32 130
Msa0534610 AT1G21860.1 25.376 532 338 17 14 538 17 496 6.96e-32 130
Msa0534610 AT4G37160.1 24.352 579 372 15 4 572 12 534 1.17e-31 129
Msa0534610 AT5G48450.2 23.836 537 367 15 14 547 17 514 1.45e-31 129
Msa0534610 AT5G48450.1 23.836 537 367 15 14 547 17 514 2.56e-31 129
Msa0534610 AT1G75790.1 24.564 574 371 19 14 572 15 541 1.48e-30 126
Msa0534610 AT1G21850.1 25.151 497 313 16 49 538 52 496 8.21e-30 124
Msa0534610 AT2G23630.2 24.771 545 364 13 29 572 38 537 2.45e-29 122
Msa0534610 AT2G23630.1 24.771 545 364 13 29 572 33 532 2.87e-29 122
Msa0534610 AT5G66920.1 23.835 558 354 14 28 572 39 538 9.82e-29 120
Msa0534610 AT1G55560.1 30.612 294 194 5 28 320 28 312 3.18e-28 119
Msa0534610 AT3G13400.2 24.366 513 336 13 28 532 29 497 2.61e-27 116
Msa0534610 AT3G13400.1 24.366 513 336 13 28 532 29 497 2.61e-27 116
Msa0534610 AT5G21105.2 25.656 343 226 5 211 550 51 367 7.67e-27 113
Msa0534610 AT4G22010.1 24.767 537 336 17 49 572 49 530 1.29e-26 114
Msa0534610 AT4G37160.2 28.037 321 213 5 4 323 12 315 4.25e-26 111
Msa0534610 AT1G55570.1 24.105 531 355 12 12 532 8 500 8.42e-26 112
Msa0534610 AT3G13390.1 25.245 511 341 15 28 535 30 502 4.49e-25 109
Msa0534610 AT4G28090.1 23.600 500 322 16 43 532 45 494 3.32e-21 97.8
Msa0534610 AT4G38420.2 28.405 257 160 7 37 285 41 281 5.52e-18 87.4
Msa0534610 AT4G38420.3 28.405 257 160 7 37 285 41 281 1.13e-17 86.7
Msa0534610 AT4G38420.1 28.405 257 160 7 37 285 41 281 1.81e-17 86.3

Find 146 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATAAAATGTGTCATATT+TGG 0.185163 4_1:-10118663 None:intergenic
TTAAACATTATGCAGGTATT+TGG 0.193690 4_1:+10123760 Msa0534610:intron
GGGCATTGGGTTACATATTC+TGG 0.208610 4_1:-10120877 None:intergenic
ACATACCAATTTAACTTAAC+TGG 0.220713 4_1:+10120922 Msa0534610:CDS
AATTCAAATGGTGGTTGATT+TGG 0.228784 4_1:-10123338 None:intergenic
GAAAGAATAGGCAAACATTT+TGG 0.246426 4_1:-10124030 None:intergenic
AATTAACTCAAGAATTTATA+AGG 0.257962 4_1:-10123926 None:intergenic
TCATGGTTATCAGGCATAAT+TGG 0.277334 4_1:-10122512 None:intergenic
TGGGTTTAACTTTCATGTTT+TGG 0.277831 4_1:+10123523 Msa0534610:CDS
TCAATTTGATAAATGTTTAC+AGG 0.279128 4_1:-10124077 None:intergenic
GAACACATGTTCATAACTTT+TGG 0.285867 4_1:+10123146 Msa0534610:CDS
CTTGTCCAGTTAAGTTAAAT+TGG 0.293953 4_1:-10120927 None:intergenic
GTCTTCACCTTAGTTGATTT+AGG 0.296358 4_1:-10123419 None:intergenic
TCAACAATTAATGGCTTATC+TGG 0.303948 4_1:+10121523 Msa0534610:CDS
TCAAATGCATCAACAATTAA+TGG 0.327150 4_1:+10121514 Msa0534610:CDS
GGGACTTCTTGATAAACCTT+TGG 0.333644 4_1:-10121048 None:intergenic
TCAGGCATAATTGGCATTAT+AGG 0.339643 4_1:-10122503 None:intergenic
CCTACAATCTTACTCTTCAT+TGG 0.348502 4_1:+10119613 Msa0534610:CDS
TCCTCTAGTGGTTATGTTAG+TGG 0.350570 4_1:-10122444 None:intergenic
AATCATCCTATTCATATTCA+TGG 0.354176 4_1:+10123503 Msa0534610:CDS
CACCAGATGGTATTGGGCAT+TGG 0.364496 4_1:-10120891 None:intergenic
AGCTGCCACCAGATGGTATT+GGG 0.364532 4_1:-10120897 None:intergenic
CTACAAAATACAGCTATTCT+TGG 0.369525 4_1:+10123473 Msa0534610:CDS
TAATTATACTGACCCTTCAT+TGG 0.373770 4_1:+10123361 Msa0534610:CDS
TTGGCTTGTACATTGTCATT+TGG 0.376918 4_1:+10123779 Msa0534610:CDS
GCAAATGCAAGTGGCTTAGA+AGG 0.379879 4_1:-10123395 None:intergenic
CCCTCTCGAGCATTAATTGT+TGG 0.386584 4_1:-10119555 None:intergenic
AAAACAATACCAAGCAATAT+GGG 0.389972 4_1:-10121069 None:intergenic
GTTGGTGTATCATGGTTATC+AGG 0.392956 4_1:-10122521 None:intergenic
GACATTGTGGTGCTTGCGCC+TGG 0.400629 4_1:+10122332 Msa0534610:CDS
AATTGAAGACTTACGATCTT+GGG 0.401325 4_1:-10121563 None:intergenic
ACCAGATGGTATTGGGCATT+GGG 0.403258 4_1:-10120890 None:intergenic
TCATCTACTTTACGTGGAAC+TGG 0.407962 4_1:-10123125 None:intergenic
TACAAAATACAGCTATTCTT+GGG 0.408559 4_1:+10123474 Msa0534610:CDS
TAATTGAAGACTTACGATCT+TGG 0.410547 4_1:-10121564 None:intergenic
TAGCTACAACTGTGAAACTA+TGG 0.411298 4_1:-10122277 None:intergenic
TCATTCAATTTCTAACTCCT+TGG 0.414307 4_1:+10120845 Msa0534610:CDS
TCCTCCCACTGGCACAGAAA+TGG 0.414706 4_1:-10123621 None:intergenic
TCCTGGTAAGGTTCCATTGA+CGG 0.426598 4_1:-10119532 None:intergenic
TTTCTGTGCCAGTGGGAGGA+TGG 0.429026 4_1:+10123624 Msa0534610:CDS
TTAAACCCATGAATATGAAT+AGG 0.429980 4_1:-10123509 None:intergenic
GGACTTCTTGATAAACCTTT+GGG 0.434862 4_1:-10121047 None:intergenic
GTTGAGAATGGGCCTACTCT+TGG 0.437907 4_1:+10123840 Msa0534610:CDS
CTAGTCGTGGCTTAATGATA+AGG 0.437923 4_1:-10121008 None:intergenic
TCAAGAGAACGTCAACGGTT+TGG 0.441613 4_1:-10122355 None:intergenic
TTTGCTCTTCCTAGGGGTAG+AGG 0.443734 4_1:+10123257 Msa0534610:CDS
CCAATGAAGAGTAAGATTGT+AGG 0.448345 4_1:-10119613 None:intergenic
GAAGCTAATAGAAGAGCAAA+AGG 0.450795 4_1:-10118699 None:intergenic
TTCTGTGCCAGTGGGAGGAT+GGG 0.454325 4_1:+10123625 Msa0534610:CDS
CACTATGATGTTGAAAGAAT+AGG 0.456772 4_1:-10124042 None:intergenic
CAGGTTATCACTGCCGTCAA+TGG 0.458437 4_1:+10119519 Msa0534610:CDS
TTCCTACGCGCTACTGTCCA+TGG 0.459320 4_1:+10120982 Msa0534610:CDS
ATATATACAGGTTTAACAAC+TGG 0.461888 4_1:+10123089 Msa0534610:intron
TTTAACAACTGGTCCACATT+GGG 0.462574 4_1:+10123100 Msa0534610:CDS
ATTGATAATGCGTAGTAAGT+AGG 0.465795 4_1:-10122216 None:intergenic
AGAGGAGTTGTTGTTTATGA+TGG 0.466459 4_1:+10122461 Msa0534610:CDS
TTATATCATACCTGTAACAT+TGG 0.468461 4_1:-10122561 None:intergenic
ATTTGCACCTAAATCAACTA+AGG 0.469161 4_1:+10123412 Msa0534610:CDS
TAGCTGCCACCAGATGGTAT+TGG 0.469636 4_1:-10120898 None:intergenic
TCAGTATAATTAAATTCAAA+TGG 0.470555 4_1:-10123350 None:intergenic
CTTGATAAACCTTTGGGAAG+GGG 0.471972 4_1:-10121041 None:intergenic
GCCGTCAATGGAACCTTACC+AGG 0.472419 4_1:+10119531 Msa0534610:CDS
GGATTAACAAAGTTAAACTT+TGG 0.476313 4_1:-10123584 None:intergenic
AGTTATTGATTCTTATTACA+TGG 0.478409 4_1:+10122388 Msa0534610:CDS
TTTCTAACTCCTTGGTCAGA+TGG 0.480191 4_1:+10120853 Msa0534610:CDS
GAAATGGTGTTACGCATTTG+AGG 0.480620 4_1:-10123605 None:intergenic
TGAGAGAAATGCAACAGATA+CGG 0.485761 4_1:+10121474 Msa0534610:CDS
ATTAAATTGCAGGGGAGTGG+TGG 0.485870 4_1:+10121431 Msa0534610:intron
ATCATCCTATTCATATTCAT+GGG 0.488322 4_1:+10123504 Msa0534610:CDS
ATTAATTGTTGGTCCTGGTA+AGG 0.491356 4_1:-10119544 None:intergenic
CGAGCATTAATTGTTGGTCC+TGG 0.493195 4_1:-10119549 None:intergenic
GAGAGAAATGCAACAGATAC+GGG 0.494209 4_1:+10121475 Msa0534610:CDS
TCAGCGATTGTGCCGTCCAC+AGG 0.494835 4_1:+10119500 Msa0534610:CDS
GGGTAGAGGTTTCTCAATGT+TGG 0.495591 4_1:+10123271 Msa0534610:CDS
CAATTTGATAAATGTTTACA+GGG 0.497563 4_1:-10124076 None:intergenic
GGAAGATTAGCTGGTGGAGG+AGG 0.498166 4_1:-10123873 None:intergenic
CAAGAAGTCCCCATATTGCT+TGG 0.500129 4_1:+10121060 Msa0534610:CDS
AGAACGTCAACGGTTTGGCC+AGG 0.501113 4_1:-10122350 None:intergenic
ATTTGGAAACACACTTGCCA+TGG 0.502328 4_1:+10123796 Msa0534610:CDS
TAGTTTCACAGTTGTAGCTA+TGG 0.504059 4_1:+10122280 Msa0534610:CDS
TGTGAGCTGTTGGTGTATCA+TGG 0.504650 4_1:-10122529 None:intergenic
TTTGGAAACACACTTGCCAT+GGG 0.506002 4_1:+10123797 Msa0534610:CDS
GGATCAACATTAGCCAATGA+AGG 0.506492 4_1:-10123374 None:intergenic
ACAACACAACGACCTGGTCC+TGG 0.509215 4_1:-10123194 None:intergenic
ATTTGCTTCTGCTGCTATCG+TGG 0.515893 4_1:+10118725 Msa0534610:CDS
CAACACTTAGGAAGATTAGC+TGG 0.518544 4_1:-10123882 None:intergenic
TTCTTGATAAACCTTTGGGA+AGG 0.519043 4_1:-10121043 None:intergenic
AAAAGATTCATTGTTCATGT+TGG 0.521543 4_1:-10123237 None:intergenic
CAATGAAGAGTAAGATTGTA+GGG 0.523403 4_1:-10119612 None:intergenic
AGATTTCAAGCAAATAATCC+AGG 0.524094 4_1:+10123656 Msa0534610:CDS
ACCCAATGCCCAATACCATC+TGG 0.526254 4_1:+10120889 Msa0534610:CDS
TAGTCGTGGCTTAATGATAA+GGG 0.527616 4_1:-10121007 None:intergenic
GTAATTAAATTGCAGGGGAG+TGG 0.530526 4_1:+10121428 Msa0534610:intron
AAACTTTGGTTCATCTCTAG+TGG 0.533014 4_1:-10123570 None:intergenic
CTTATCATTAAGCCACGACT+AGG 0.535485 4_1:+10121009 Msa0534610:CDS
TCTTGATAAACCTTTGGGAA+GGG 0.536055 4_1:-10121042 None:intergenic
ACCAACAATTAATGCTCGAG+AGG 0.542459 4_1:+10119554 Msa0534610:CDS
TGTAATAAGAATCAATAACT+TGG 0.543985 4_1:-10122385 None:intergenic
CCATACCGTTCTTCAAGTGT+TGG 0.544154 4_1:+10122419 Msa0534610:CDS
GTTTAACAACTGGTCCACAT+TGG 0.554162 4_1:+10123099 Msa0534610:CDS
GTTGATGCATTTGACTCCGC+TGG 0.558250 4_1:-10121505 None:intergenic
CTAGAGCAATGCATCAATCC+AGG 0.561417 4_1:+10123176 Msa0534610:CDS
ATTTAACTTAACTGGACAAG+AGG 0.562624 4_1:+10120930 Msa0534610:CDS
GATGCATTTGACTCCGCTGG+TGG 0.562919 4_1:-10121502 None:intergenic
GACAAGAGGGTACACTTTGG+TGG 0.569591 4_1:+10120944 Msa0534610:CDS
CAATGCATCAATCCAGGACC+AGG 0.571950 4_1:+10123182 Msa0534610:CDS
AAATAATGTACTCAACACTT+AGG 0.572543 4_1:-10123894 None:intergenic
CGTGGAACTGGAACCCAATG+TGG 0.578205 4_1:-10123113 None:intergenic
GTATAATTAAATTCAAATGG+TGG 0.578940 4_1:-10123347 None:intergenic
ACCATTTCTGTGCCAGTGGG+AGG 0.579775 4_1:+10123620 Msa0534610:CDS
TTAGGAAGATTAGCTGGTGG+AGG 0.587146 4_1:-10123876 None:intergenic
GATTTAGGTGCAAATGCAAG+TGG 0.589023 4_1:-10123404 None:intergenic
CTGGACAAGAGGGTACACTT+TGG 0.590545 4_1:+10120941 Msa0534610:CDS
ATAACAGCCCATCCTCCCAC+TGG 0.590871 4_1:-10123632 None:intergenic
TACAAGCTCAAAGTGAAGCA+AGG 0.595483 4_1:+10122188 Msa0534610:CDS
ATGTGTTCATCTACTTTACG+TGG 0.597501 4_1:-10123131 None:intergenic
ACACTATAACACTGACATTG+TGG 0.600970 4_1:+10122319 Msa0534610:CDS
GTGTAAAACTTGTGAGCTGT+TGG 0.601475 4_1:-10122539 None:intergenic
ATGCATTTGACTCCGCTGGT+GGG 0.601923 4_1:-10121501 None:intergenic
TAACACCATTTCTGTGCCAG+TGG 0.602566 4_1:+10123616 Msa0534610:CDS
ATAAACAACAACTCCTCTAG+TGG 0.606065 4_1:-10122456 None:intergenic
GGCACTGTTGTTCCAAGAGT+AGG 0.606785 4_1:-10123852 None:intergenic
CCAACACTTGAAGAACGGTA+TGG 0.608437 4_1:-10122419 None:intergenic
TATGTATAGCTGCCACCAGA+TGG 0.609465 4_1:-10120904 None:intergenic
TTTAACTTAACTGGACAAGA+GGG 0.611861 4_1:+10120931 Msa0534610:CDS
ATTGAGAAACCTCTACCCCT+AGG 0.617029 4_1:-10123266 None:intergenic
TGGTTCCAACACTTGAAGAA+CGG 0.619947 4_1:-10122424 None:intergenic
TGGAAACACACTTGCCATGG+GGG 0.620681 4_1:+10123799 Msa0534610:CDS
ACCACTAACATAACCACTAG+AGG 0.625523 4_1:+10122443 Msa0534610:CDS
TCGCTCATACCCCTTCCCAA+AGG 0.634666 4_1:+10121032 Msa0534610:CDS
ACAAAATACAGCTATTCTTG+GGG 0.644652 4_1:+10123475 Msa0534610:CDS
AAACAATACCAAGCAATATG+GGG 0.644866 4_1:-10121068 None:intergenic
GATCAACATTAGCCAATGAA+GGG 0.645575 4_1:-10123373 None:intergenic
CGGCAGTGATAACCTGTGGA+CGG 0.647467 4_1:-10119512 None:intergenic
TTGGAAACACACTTGCCATG+GGG 0.647612 4_1:+10123798 Msa0534610:CDS
AACACCATTTCTGTGCCAGT+GGG 0.650163 4_1:+10123617 Msa0534610:CDS
GGGTATGAGCGACCTAGTCG+TGG 0.653043 4_1:-10121021 None:intergenic
CACTTAGGAAGATTAGCTGG+TGG 0.654885 4_1:-10123879 None:intergenic
TTGACGGCAGTGATAACCTG+TGG 0.667579 4_1:-10119516 None:intergenic
CACCATGGACAGTAGCGCGT+AGG 0.667826 4_1:-10120984 None:intergenic
CCAACAATTAATGCTCGAGA+GGG 0.669858 4_1:+10119555 Msa0534610:CDS
CAATGCCCAATACCATCTGG+TGG 0.674666 4_1:+10120892 Msa0534610:CDS
GCTTAATGATAAGGGCACCA+TGG 0.676516 4_1:-10120999 None:intergenic
TTTAGTACAACACAACGACC+TGG 0.677465 4_1:-10123200 None:intergenic
TATTCTGGACCATCTGACCA+AGG 0.704441 4_1:-10120862 None:intergenic
AGATACGGGTAACCCACCAG+CGG 0.756602 4_1:+10121489 Msa0534610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTTTTAAGTAAAATAA+AGG - chr4_1:10118844-10118863 None:intergenic 10.0%
!!! AATTAATTTTATCATAAAAG+AGG + chr4_1:10120356-10120375 Msa0534610:intron 10.0%
!!! ATTAATTTTATCATAAAAGA+GGG + chr4_1:10120357-10120376 Msa0534610:intron 10.0%
!! ATATATAATTAGATAAACAA+AGG + chr4_1:10120575-10120594 Msa0534610:intron 10.0%
!!! CTATTTTTTTTTTATTGAAA+GGG - chr4_1:10120741-10120760 None:intergenic 10.0%
!!! ACTATTTTTTTTTTATTGAA+AGG - chr4_1:10120742-10120761 None:intergenic 10.0%
!! AAATATGAATTCTTATAATT+AGG - chr4_1:10121130-10121149 None:intergenic 10.0%
!!! AATTGTTTAGAATAATTTTT+TGG - chr4_1:10121394-10121413 None:intergenic 10.0%
!! TTCAATTAATTAACAAATTT+GGG + chr4_1:10121579-10121598 Msa0534610:intron 10.0%
!!! ATTTAATTTATATGCAATTT+TGG + chr4_1:10122655-10122674 Msa0534610:intron 10.0%
!! TAAATTTCCATATATTCTAA+AGG - chr4_1:10118805-10118824 None:intergenic 15.0%
!! TATAACTAGATTGAAATAAA+AGG + chr4_1:10119054-10119073 Msa0534610:intron 15.0%
!! TGTTACTAAACTTTATAATA+TGG + chr4_1:10119413-10119432 Msa0534610:intron 15.0%
!!! TTAATTTTATTTTGATATCC+AGG + chr4_1:10119461-10119480 Msa0534610:intron 15.0%
!!! TTCTCTAATATTTTTATTGA+GGG - chr4_1:10119658-10119677 None:intergenic 15.0%
!!! TTTCTCTAATATTTTTATTG+AGG - chr4_1:10119659-10119678 None:intergenic 15.0%
!!! TTACATGATCATTATTTTAA+CGG + chr4_1:10119818-10119837 Msa0534610:intron 15.0%
!!! ATTTTTTACATAGTTGTTTT+TGG - chr4_1:10120126-10120145 None:intergenic 15.0%
!! AAAGAGAATATTCTTAAAAT+AGG - chr4_1:10120226-10120245 None:intergenic 15.0%
!!! TTATTTTTCTCATATGTATA+GGG + chr4_1:10120763-10120782 Msa0534610:intron 15.0%
!! TGTTAAATGTAATTCTAATT+TGG + chr4_1:10120794-10120813 Msa0534610:intron 15.0%
!! ATATATAGTAATTAAATTGC+AGG + chr4_1:10121421-10121440 Msa0534610:intron 15.0%
!! TATATAGTAATTAAATTGCA+GGG + chr4_1:10121422-10121441 Msa0534610:intron 15.0%
!! CTTCAATTAATTAACAAATT+TGG + chr4_1:10121578-10121597 Msa0534610:intron 15.0%
!! TCATATCTTAAAATATAGTT+TGG - chr4_1:10122035-10122054 None:intergenic 15.0%
!! TGTGTATATATTATTAACTT+TGG + chr4_1:10122625-10122644 Msa0534610:intron 15.0%
!!! AATGTGTATATCTTTTTTTT+AGG + chr4_1:10123028-10123047 Msa0534610:intron 15.0%
!!! GAAATTTTGTATATATATAC+AGG + chr4_1:10123077-10123096 Msa0534610:intron 15.0%
!! TCAGTATAATTAAATTCAAA+TGG - chr4_1:10123353-10123372 None:intergenic 15.0%
!! AATTAACTCAAGAATTTATA+AGG - chr4_1:10123929-10123948 None:intergenic 15.0%
!! AACATAAAATGTGTCATATT+TGG - chr4_1:10118666-10118685 None:intergenic 20.0%
!!! ATTTCTTCCTTTAGAATATA+TGG + chr4_1:10118795-10118814 Msa0534610:intron 20.0%
!! CTAGTTATAAAGATTTACTA+TGG - chr4_1:10119043-10119062 None:intergenic 20.0%
!! AAGAAAAGATTAAAACAAGT+TGG - chr4_1:10119289-10119308 None:intergenic 20.0%
!!! TAATATTTTTATTGAGGGTA+AGG - chr4_1:10119653-10119672 None:intergenic 20.0%
!! GAAATAAATTAAACAAACCA+TGG + chr4_1:10119675-10119694 Msa0534610:intron 20.0%
!!! ATCAATTTTTTGACGAATTA+TGG - chr4_1:10120545-10120564 None:intergenic 20.0%
!! ATACTATTGTTAAAATGAGA+TGG - chr4_1:10120709-10120728 None:intergenic 20.0%
!!! GTTATTTTTCTCATATGTAT+AGG + chr4_1:10120762-10120781 Msa0534610:intron 20.0%
!!! GTTTTTTCACATTACATATT+TGG + chr4_1:10121087-10121106 Msa0534610:intron 20.0%
!!! TAAGAATTCATATTTTTCTG+TGG + chr4_1:10121135-10121154 Msa0534610:intron 20.0%
!! ATATAGTAATTAAATTGCAG+GGG + chr4_1:10121423-10121442 Msa0534610:intron 20.0%
!!! TCTATTTTAGTAGATGAAAT+TGG + chr4_1:10121618-10121637 Msa0534610:intron 20.0%
!! CATGTAAAAGTTAAGAAATT+TGG - chr4_1:10121701-10121720 None:intergenic 20.0%
!!! CTTTCTTTTACAATAAACTT+AGG + chr4_1:10121733-10121752 Msa0534610:intron 20.0%
!! AAATTCAATTATCTTTGGTA+AGG - chr4_1:10121968-10121987 None:intergenic 20.0%
!!! ATCTGAAATTCAATTATCTT+TGG - chr4_1:10121973-10121992 None:intergenic 20.0%
!!! TCATGATTGTTTTAACATTT+TGG + chr4_1:10122143-10122162 Msa0534610:intron 20.0%
!! TGTAATAAGAATCAATAACT+TGG - chr4_1:10122388-10122407 None:intergenic 20.0%
!!! AGTTATTGATTCTTATTACA+TGG + chr4_1:10122388-10122407 Msa0534610:CDS 20.0%
!! TTACAGGTATGATATAAATA+GGG + chr4_1:10122567-10122586 Msa0534610:intron 20.0%
!! CACCTAATTATTATCAAATT+CGG + chr4_1:10122709-10122728 Msa0534610:intron 20.0%
!!! ATCCGAATTTGATAATAATT+AGG - chr4_1:10122714-10122733 None:intergenic 20.0%
!! TTCAATGATCATAAATATAC+TGG + chr4_1:10122843-10122862 Msa0534610:intron 20.0%
!! TACTCTATAGTGAAAATATT+TGG + chr4_1:10122990-10123009 Msa0534610:intron 20.0%
!! GTATAATTAAATTCAAATGG+TGG - chr4_1:10123350-10123369 None:intergenic 20.0%
!!! ATTGACAAAAAGTGTAATAA+AGG + chr4_1:10124007-10124026 Msa0534610:three_prime_UTR 20.0%
!! CAATTTGATAAATGTTTACA+GGG - chr4_1:10124079-10124098 None:intergenic 20.0%
!! TCAATTTGATAAATGTTTAC+AGG - chr4_1:10124080-10124099 None:intergenic 20.0%
!! ATTTTATGTTTTCTCTAGCA+TGG + chr4_1:10118676-10118695 Msa0534610:CDS 25.0%
! TACTTTCAATGTATTGTTGA+TGG + chr4_1:10118752-10118771 Msa0534610:intron 25.0%
! ACTTTCAATGTATTGTTGAT+GGG + chr4_1:10118753-10118772 Msa0534610:intron 25.0%
! ATAAAGGAAGAAAGCTATAT+AGG - chr4_1:10118828-10118847 None:intergenic 25.0%
! TATGATCCACATGAAAAAAT+AGG + chr4_1:10118936-10118955 Msa0534610:intron 25.0%
! ATGATCCACATGAAAAAATA+GGG + chr4_1:10118937-10118956 Msa0534610:intron 25.0%
!! CAATCTATTTTCTTTTGCAA+AGG - chr4_1:10119214-10119233 None:intergenic 25.0%
! TCTAAAAAAGCTCAAACTTA+AGG - chr4_1:10119372-10119391 None:intergenic 25.0%
! TAATTCTTGAATTCAAACCA+TGG - chr4_1:10119695-10119714 None:intergenic 25.0%
! AAATACAAGAAACGTTTAAC+AGG - chr4_1:10119740-10119759 None:intergenic 25.0%
!!! TTTCTTTTCCATTTTTTCAG+AGG + chr4_1:10119999-10120018 Msa0534610:intron 25.0%
!! AGTTTTTGACACAAAGTTAT+CGG + chr4_1:10120167-10120186 Msa0534610:intron 25.0%
! TTTAAAAACATATGAGACTC+CGG + chr4_1:10120432-10120451 Msa0534610:intron 25.0%
! GGTTACATTATAAAATATGC+AGG + chr4_1:10120815-10120834 Msa0534610:intron 25.0%
!! ACATACCAATTTAACTTAAC+TGG + chr4_1:10120922-10120941 Msa0534610:CDS 25.0%
! AAAACAATACCAAGCAATAT+GGG - chr4_1:10121072-10121091 None:intergenic 25.0%
! AAAAACAATACCAAGCAATA+TGG - chr4_1:10121073-10121092 None:intergenic 25.0%
! CTATCAAACGTCAATAAATA+TGG + chr4_1:10121300-10121319 Msa0534610:intron 25.0%
! AGAAATGAATTCAAATGTCA+TGG - chr4_1:10121340-10121359 None:intergenic 25.0%
! TCAAATGCATCAACAATTAA+TGG + chr4_1:10121514-10121533 Msa0534610:CDS 25.0%
! TCTCCACTTATTTACTTAAA+GGG - chr4_1:10121841-10121860 None:intergenic 25.0%
! TTCTCCACTTATTTACTTAA+AGG - chr4_1:10121842-10121861 None:intergenic 25.0%
! AAGTAAATAAGTGGAGAATA+AGG + chr4_1:10121844-10121863 Msa0534610:intron 25.0%
! AGTAAATAAGTGGAGAATAA+GGG + chr4_1:10121845-10121864 Msa0534610:intron 25.0%
! AAAACAATCATGAGTCATAT+GGG - chr4_1:10122136-10122155 None:intergenic 25.0%
! TAAAACAATCATGAGTCATA+TGG - chr4_1:10122137-10122156 None:intergenic 25.0%
! TTATATCATACCTGTAACAT+TGG - chr4_1:10122564-10122583 None:intergenic 25.0%
! GTTACAGGTATGATATAAAT+AGG + chr4_1:10122566-10122585 Msa0534610:intron 25.0%
!!! ATTTTGGCGTAAATTAACTT+TGG + chr4_1:10122671-10122690 Msa0534610:intron 25.0%
! CATTTACTAATTGCAAGATT+CGG + chr4_1:10122740-10122759 Msa0534610:intron 25.0%
! ATATATACAGGTTTAACAAC+TGG + chr4_1:10123089-10123108 Msa0534610:intron 25.0%
!! ATGTTCATAACTTTTGGTAT+TGG + chr4_1:10123152-10123171 Msa0534610:CDS 25.0%
! AAAAGATTCATTGTTCATGT+TGG - chr4_1:10123240-10123259 None:intergenic 25.0%
!! GCATTTTACAACAATATTAG+TGG + chr4_1:10123296-10123315 Msa0534610:CDS 25.0%
! TACAAAATACAGCTATTCTT+GGG + chr4_1:10123474-10123493 Msa0534610:CDS 25.0%
! AATCATCCTATTCATATTCA+TGG + chr4_1:10123503-10123522 Msa0534610:CDS 25.0%
! ATCATCCTATTCATATTCAT+GGG + chr4_1:10123504-10123523 Msa0534610:CDS 25.0%
! TTAAACCCATGAATATGAAT+AGG - chr4_1:10123512-10123531 None:intergenic 25.0%
! GGATTAACAAAGTTAAACTT+TGG - chr4_1:10123587-10123606 None:intergenic 25.0%
! TTCTACTTTAAACATTATGC+AGG + chr4_1:10123753-10123772 Msa0534610:intron 25.0%
! TTAAACATTATGCAGGTATT+TGG + chr4_1:10123760-10123779 Msa0534610:intron 25.0%
! AAATAATGTACTCAACACTT+AGG - chr4_1:10123897-10123916 None:intergenic 25.0%
AGAAATGAGAGATGTAGAAA+AGG - chr4_1:10118781-10118800 None:intergenic 30.0%
TGTATAGTCCTTATGTAAGA+AGG + chr4_1:10118883-10118902 Msa0534610:intron 30.0%
!!! GTTTTCCCTATTTTTTCATG+TGG - chr4_1:10118945-10118964 None:intergenic 30.0%
AAAGCTCAAACTTAAGGTAA+TGG - chr4_1:10119366-10119385 None:intergenic 30.0%
CAATGAAGAGTAAGATTGTA+GGG - chr4_1:10119615-10119634 None:intergenic 30.0%
TCAACATTATACTACATGCA+TGG - chr4_1:10119782-10119801 None:intergenic 30.0%
! AGCAATATCTTCTTGTTTCT+AGG - chr4_1:10119940-10119959 None:intergenic 30.0%
TTAAAAACATATGAGACTCC+GGG + chr4_1:10120433-10120452 Msa0534610:intron 30.0%
! GCTAAATTTTCTTTCTTTCC+CGG - chr4_1:10120454-10120473 None:intergenic 30.0%
CATTATAAAATATGCAGGCA+TGG + chr4_1:10120820-10120839 Msa0534610:intron 30.0%
TCATTCAATTTCTAACTCCT+TGG + chr4_1:10120845-10120864 Msa0534610:CDS 30.0%
CTTGTCCAGTTAAGTTAAAT+TGG - chr4_1:10120930-10120949 None:intergenic 30.0%
! ATTTAACTTAACTGGACAAG+AGG + chr4_1:10120930-10120949 Msa0534610:CDS 30.0%
! TTTAACTTAACTGGACAAGA+GGG + chr4_1:10120931-10120950 Msa0534610:CDS 30.0%
AAACAATACCAAGCAATATG+GGG - chr4_1:10121071-10121090 None:intergenic 30.0%
TCAACAATTAATGGCTTATC+TGG + chr4_1:10121523-10121542 Msa0534610:CDS 30.0%
AATTGAAGACTTACGATCTT+GGG - chr4_1:10121566-10121585 None:intergenic 30.0%
TAATTGAAGACTTACGATCT+TGG - chr4_1:10121567-10121586 None:intergenic 30.0%
TTCCCCTTTAAGTAAATAAG+TGG + chr4_1:10121835-10121854 Msa0534610:intron 30.0%
CTCCACTTATTTACTTAAAG+GGG - chr4_1:10121840-10121859 None:intergenic 30.0%
! TTTTACATTGCAGTATGAAG+AGG - chr4_1:10121882-10121901 None:intergenic 30.0%
TTCAATTATCTTTGGTAAGG+AGG - chr4_1:10121965-10121984 None:intergenic 30.0%
!! ATTGATAATGCGTAGTAAGT+AGG - chr4_1:10122219-10122238 None:intergenic 30.0%
!! CATTATAGGTGCTTTTGTTT+GGG - chr4_1:10122492-10122511 None:intergenic 30.0%
! AAGTTTTACACCAATGTTAC+AGG + chr4_1:10122551-10122570 Msa0534610:CDS 30.0%
TGATATAAATAGGGTTTCAC+TGG + chr4_1:10122576-10122595 Msa0534610:intron 30.0%
! GAACACATGTTCATAACTTT+TGG + chr4_1:10123146-10123165 Msa0534610:CDS 30.0%
AATTCAAATGGTGGTTGATT+TGG - chr4_1:10123341-10123360 None:intergenic 30.0%
TAATTATACTGACCCTTCAT+TGG + chr4_1:10123361-10123380 Msa0534610:CDS 30.0%
ATTTGCACCTAAATCAACTA+AGG + chr4_1:10123412-10123431 Msa0534610:CDS 30.0%
CTACAAAATACAGCTATTCT+TGG + chr4_1:10123473-10123492 Msa0534610:CDS 30.0%
ACAAAATACAGCTATTCTTG+GGG + chr4_1:10123475-10123494 Msa0534610:CDS 30.0%
!! TGGGTTTAACTTTCATGTTT+TGG + chr4_1:10123523-10123542 Msa0534610:CDS 30.0%
AGATTTCAAGCAAATAATCC+AGG + chr4_1:10123656-10123675 Msa0534610:CDS 30.0%
ACATGTAAATAAAACCAACC+TGG - chr4_1:10123677-10123696 None:intergenic 30.0%
! GAAAGAATAGGCAAACATTT+TGG - chr4_1:10124033-10124052 None:intergenic 30.0%
CACTATGATGTTGAAAGAAT+AGG - chr4_1:10124045-10124064 None:intergenic 30.0%
GAAGCTAATAGAAGAGCAAA+AGG - chr4_1:10118702-10118721 None:intergenic 35.0%
ACACTACACCTTCTTACATA+AGG - chr4_1:10118894-10118913 None:intergenic 35.0%
ATTAATTGTTGGTCCTGGTA+AGG - chr4_1:10119547-10119566 None:intergenic 35.0%
CCAATGAAGAGTAAGATTGT+AGG - chr4_1:10119616-10119635 None:intergenic 35.0%
CCTACAATCTTACTCTTCAT+TGG + chr4_1:10119613-10119632 Msa0534610:CDS 35.0%
TACAAGAAACGTTTAACAGG+AGG - chr4_1:10119737-10119756 None:intergenic 35.0%
! AGCAAGATCCTCTGAAAAAA+TGG - chr4_1:10120010-10120029 None:intergenic 35.0%
TCATAAAAGAGGGTGAAGTT+TGG + chr4_1:10120367-10120386 Msa0534610:intron 35.0%
TAGTCGTGGCTTAATGATAA+GGG - chr4_1:10121010-10121029 None:intergenic 35.0%
! TCTTGATAAACCTTTGGGAA+GGG - chr4_1:10121045-10121064 None:intergenic 35.0%
! TTCTTGATAAACCTTTGGGA+AGG - chr4_1:10121046-10121065 None:intergenic 35.0%
GGACTTCTTGATAAACCTTT+GGG - chr4_1:10121050-10121069 None:intergenic 35.0%
TGAGAGAAATGCAACAGATA+CGG + chr4_1:10121474-10121493 Msa0534610:CDS 35.0%
ATTATCTTTGGTAAGGAGGT+TGG - chr4_1:10121961-10121980 None:intergenic 35.0%
! GTGATGTTAACTTGAGATCT+TGG - chr4_1:10122013-10122032 None:intergenic 35.0%
TAGCTACAACTGTGAAACTA+TGG - chr4_1:10122280-10122299 None:intergenic 35.0%
TAGTTTCACAGTTGTAGCTA+TGG + chr4_1:10122280-10122299 Msa0534610:CDS 35.0%
ACACTATAACACTGACATTG+TGG + chr4_1:10122319-10122338 Msa0534610:CDS 35.0%
!! AGTTTTCAAGAGAACGTCAA+CGG - chr4_1:10122363-10122382 None:intergenic 35.0%
ATAAACAACAACTCCTCTAG+TGG - chr4_1:10122459-10122478 None:intergenic 35.0%
!! AGAGGAGTTGTTGTTTATGA+TGG + chr4_1:10122461-10122480 Msa0534610:CDS 35.0%
! GCATTATAGGTGCTTTTGTT+TGG - chr4_1:10122493-10122512 None:intergenic 35.0%
TCAGGCATAATTGGCATTAT+AGG - chr4_1:10122506-10122525 None:intergenic 35.0%
TCATGGTTATCAGGCATAAT+TGG - chr4_1:10122515-10122534 None:intergenic 35.0%
TTTAACAACTGGTCCACATT+GGG + chr4_1:10123100-10123119 Msa0534610:CDS 35.0%
ATGTGTTCATCTACTTTACG+TGG - chr4_1:10123134-10123153 None:intergenic 35.0%
! ATGAATCTTTTGCTCTTCCT+AGG + chr4_1:10123249-10123268 Msa0534610:CDS 35.0%
! TGAATCTTTTGCTCTTCCTA+GGG + chr4_1:10123250-10123269 Msa0534610:CDS 35.0%
GATCAACATTAGCCAATGAA+GGG - chr4_1:10123376-10123395 None:intergenic 35.0%
! GTCTTCACCTTAGTTGATTT+AGG - chr4_1:10123422-10123441 None:intergenic 35.0%
!!! AACTTTCATGTTTTGGCACA+AGG + chr4_1:10123530-10123549 Msa0534610:CDS 35.0%
! AAACTTTGGTTCATCTCTAG+TGG - chr4_1:10123573-10123592 None:intergenic 35.0%
TTCAAGCAAATAATCCAGGT+TGG + chr4_1:10123660-10123679 Msa0534610:intron 35.0%
!! TTGGCTTGTACATTGTCATT+TGG + chr4_1:10123779-10123798 Msa0534610:CDS 35.0%
GAATAGTGAAGTCTTCAACC+TGG - chr4_1:10119482-10119501 None:intergenic 40.0%
ACCAACAATTAATGCTCGAG+AGG + chr4_1:10119554-10119573 Msa0534610:CDS 40.0%
CCAACAATTAATGCTCGAGA+GGG + chr4_1:10119555-10119574 Msa0534610:CDS 40.0%
TCTTGCTAACGAGTGTTCTA+AGG + chr4_1:10120023-10120042 Msa0534610:intron 40.0%
GGAGAAAGAATTAAGACCGA+TGG - chr4_1:10120205-10120224 None:intergenic 40.0%
TTTCTAACTCCTTGGTCAGA+TGG + chr4_1:10120853-10120872 Msa0534610:CDS 40.0%
CTAGTCGTGGCTTAATGATA+AGG - chr4_1:10121011-10121030 None:intergenic 40.0%
CTTATCATTAAGCCACGACT+AGG + chr4_1:10121009-10121028 Msa0534610:CDS 40.0%
! CTTGATAAACCTTTGGGAAG+GGG - chr4_1:10121044-10121063 None:intergenic 40.0%
GGGACTTCTTGATAAACCTT+TGG - chr4_1:10121051-10121070 None:intergenic 40.0%
TTGCATGCATTCACGCAATT+AGG + chr4_1:10121165-10121184 Msa0534610:intron 40.0%
GTAATTAAATTGCAGGGGAG+TGG + chr4_1:10121428-10121447 Msa0534610:intron 40.0%
GAGAGAAATGCAACAGATAC+GGG + chr4_1:10121475-10121494 Msa0534610:CDS 40.0%
ACATTGCAGTATGAAGAGGT+TGG - chr4_1:10121878-10121897 None:intergenic 40.0%
AGGTTGGATTATGAAGAGGT+TGG - chr4_1:10121945-10121964 None:intergenic 40.0%
AAGGAGGTTGGATTATGAAG+AGG - chr4_1:10121949-10121968 None:intergenic 40.0%
TACAAGCTCAAAGTGAAGCA+AGG + chr4_1:10122188-10122207 Msa0534610:CDS 40.0%
TGGTTCCAACACTTGAAGAA+CGG - chr4_1:10122427-10122446 None:intergenic 40.0%
ACCACTAACATAACCACTAG+AGG + chr4_1:10122443-10122462 Msa0534610:CDS 40.0%
TCCTCTAGTGGTTATGTTAG+TGG - chr4_1:10122447-10122466 None:intergenic 40.0%
!! TATAGGTGCTTTTGTTTGGG+AGG - chr4_1:10122489-10122508 None:intergenic 40.0%
GTTGGTGTATCATGGTTATC+AGG - chr4_1:10122524-10122543 None:intergenic 40.0%
GTGTAAAACTTGTGAGCTGT+TGG - chr4_1:10122542-10122561 None:intergenic 40.0%
GTTTAACAACTGGTCCACAT+TGG + chr4_1:10123099-10123118 Msa0534610:CDS 40.0%
TCATCTACTTTACGTGGAAC+TGG - chr4_1:10123128-10123147 None:intergenic 40.0%
TTTAGTACAACACAACGACC+TGG - chr4_1:10123203-10123222 None:intergenic 40.0%
! GAATCTTTTGCTCTTCCTAG+GGG + chr4_1:10123251-10123270 Msa0534610:CDS 40.0%
GGATCAACATTAGCCAATGA+AGG - chr4_1:10123377-10123396 None:intergenic 40.0%
GATTTAGGTGCAAATGCAAG+TGG - chr4_1:10123407-10123426 None:intergenic 40.0%
!!! CATGTTTTGGCACAAGGTTT+TGG + chr4_1:10123536-10123555 Msa0534610:CDS 40.0%
!! GAAATGGTGTTACGCATTTG+AGG - chr4_1:10123608-10123627 None:intergenic 40.0%
ATTTGGAAACACACTTGCCA+TGG + chr4_1:10123796-10123815 Msa0534610:CDS 40.0%
TTTGGAAACACACTTGCCAT+GGG + chr4_1:10123797-10123816 Msa0534610:CDS 40.0%
!!! ATGGCTTTTGAGGTTGAGAA+TGG + chr4_1:10123828-10123847 Msa0534610:CDS 40.0%
!!! TGGCTTTTGAGGTTGAGAAT+GGG + chr4_1:10123829-10123848 Msa0534610:CDS 40.0%
CAACACTTAGGAAGATTAGC+TGG - chr4_1:10123885-10123904 None:intergenic 40.0%
ATTTGCTTCTGCTGCTATCG+TGG + chr4_1:10118725-10118744 Msa0534610:CDS 45.0%
TCCTGGTAAGGTTCCATTGA+CGG - chr4_1:10119535-10119554 None:intergenic 45.0%
CGAGCATTAATTGTTGGTCC+TGG - chr4_1:10119552-10119571 None:intergenic 45.0%
CCCTCTCGAGCATTAATTGT+TGG - chr4_1:10119558-10119577 None:intergenic 45.0%
TCGGATAGTTGATCGACCAT+CGG + chr4_1:10120186-10120205 Msa0534610:intron 45.0%
TATTCTGGACCATCTGACCA+AGG - chr4_1:10120865-10120884 None:intergenic 45.0%
!! GGGCATTGGGTTACATATTC+TGG - chr4_1:10120880-10120899 None:intergenic 45.0%
! ACCAGATGGTATTGGGCATT+GGG - chr4_1:10120893-10120912 None:intergenic 45.0%
TATGTATAGCTGCCACCAGA+TGG - chr4_1:10120907-10120926 None:intergenic 45.0%
!! GCTTAATGATAAGGGCACCA+TGG - chr4_1:10121002-10121021 None:intergenic 45.0%
CAAGAAGTCCCCATATTGCT+TGG + chr4_1:10121060-10121079 Msa0534610:CDS 45.0%
ATTAAATTGCAGGGGAGTGG+TGG + chr4_1:10121431-10121450 Msa0534610:intron 45.0%
TCAAGAGAACGTCAACGGTT+TGG - chr4_1:10122358-10122377 None:intergenic 45.0%
CCAACACTTGAAGAACGGTA+TGG - chr4_1:10122422-10122441 None:intergenic 45.0%
CCATACCGTTCTTCAAGTGT+TGG + chr4_1:10122419-10122438 Msa0534610:CDS 45.0%
TGTGAGCTGTTGGTGTATCA+TGG - chr4_1:10122532-10122551 None:intergenic 45.0%
CTAGAGCAATGCATCAATCC+AGG + chr4_1:10123176-10123195 Msa0534610:CDS 45.0%
ATTGAGAAACCTCTACCCCT+AGG - chr4_1:10123269-10123288 None:intergenic 45.0%
GGGTAGAGGTTTCTCAATGT+TGG + chr4_1:10123271-10123290 Msa0534610:CDS 45.0%
GCAAATGCAAGTGGCTTAGA+AGG - chr4_1:10123398-10123417 None:intergenic 45.0%
TAACACCATTTCTGTGCCAG+TGG + chr4_1:10123616-10123635 Msa0534610:CDS 45.0%
AACACCATTTCTGTGCCAGT+GGG + chr4_1:10123617-10123636 Msa0534610:CDS 45.0%
TTGGAAACACACTTGCCATG+GGG + chr4_1:10123798-10123817 Msa0534610:CDS 45.0%
! TTAGGAAGATTAGCTGGTGG+AGG - chr4_1:10123879-10123898 None:intergenic 45.0%
! CACTTAGGAAGATTAGCTGG+TGG - chr4_1:10123882-10123901 None:intergenic 45.0%
!! TTATTTAATAATTTATGAAT+TGG - chr4_1:10120066-10120085 None:intergenic 5.0%
!! AAACAAAATTATAAATAATA+TGG + chr4_1:10120298-10120317 Msa0534610:intron 5.0%
TTGACGGCAGTGATAACCTG+TGG - chr4_1:10119519-10119538 None:intergenic 50.0%
CAGGTTATCACTGCCGTCAA+TGG + chr4_1:10119519-10119538 Msa0534610:CDS 50.0%
ACCCAATGCCCAATACCATC+TGG + chr4_1:10120889-10120908 Msa0534610:CDS 50.0%
! CACCAGATGGTATTGGGCAT+TGG - chr4_1:10120894-10120913 None:intergenic 50.0%
CAATGCCCAATACCATCTGG+TGG + chr4_1:10120892-10120911 Msa0534610:CDS 50.0%
AGCTGCCACCAGATGGTATT+GGG - chr4_1:10120900-10120919 None:intergenic 50.0%
TAGCTGCCACCAGATGGTAT+TGG - chr4_1:10120901-10120920 None:intergenic 50.0%
! CTGGACAAGAGGGTACACTT+TGG + chr4_1:10120941-10120960 Msa0534610:CDS 50.0%
! GACAAGAGGGTACACTTTGG+TGG + chr4_1:10120944-10120963 Msa0534610:CDS 50.0%
ATGCATTTGACTCCGCTGGT+GGG - chr4_1:10121504-10121523 None:intergenic 50.0%
GTTGATGCATTTGACTCCGC+TGG - chr4_1:10121508-10121527 None:intergenic 50.0%
GTGCGTGTGTGTCAGATTCA+TGG - chr4_1:10122880-10122899 None:intergenic 50.0%
CAATGCATCAATCCAGGACC+AGG + chr4_1:10123182-10123201 Msa0534610:CDS 50.0%
TTTGCTCTTCCTAGGGGTAG+AGG + chr4_1:10123257-10123276 Msa0534610:CDS 50.0%
TGGAAACACACTTGCCATGG+GGG + chr4_1:10123799-10123818 Msa0534610:CDS 50.0%
AAAGCCATTGCTAACCCCCA+TGG - chr4_1:10123816-10123835 None:intergenic 50.0%
!! GGGGTTAGCAATGGCTTTTG+AGG + chr4_1:10123818-10123837 Msa0534610:CDS 50.0%
GTTGAGAATGGGCCTACTCT+TGG + chr4_1:10123840-10123859 Msa0534610:CDS 50.0%
GGCACTGTTGTTCCAAGAGT+AGG - chr4_1:10123855-10123874 None:intergenic 50.0%
CGGCAGTGATAACCTGTGGA+CGG - chr4_1:10119515-10119534 None:intergenic 55.0%
GCCGTCAATGGAACCTTACC+AGG + chr4_1:10119531-10119550 Msa0534610:CDS 55.0%
TTCCTACGCGCTACTGTCCA+TGG + chr4_1:10120982-10121001 Msa0534610:CDS 55.0%
TCGCTCATACCCCTTCCCAA+AGG + chr4_1:10121032-10121051 Msa0534610:CDS 55.0%
AGATACGGGTAACCCACCAG+CGG + chr4_1:10121489-10121508 Msa0534610:CDS 55.0%
GATGCATTTGACTCCGCTGG+TGG - chr4_1:10121505-10121524 None:intergenic 55.0%
! AGAACGTCAACGGTTTGGCC+AGG - chr4_1:10122353-10122372 None:intergenic 55.0%
CGTGGAACTGGAACCCAATG+TGG - chr4_1:10123116-10123135 None:intergenic 55.0%
ACAACACAACGACCTGGTCC+TGG - chr4_1:10123197-10123216 None:intergenic 55.0%
ACCATTTCTGTGCCAGTGGG+AGG + chr4_1:10123620-10123639 Msa0534610:CDS 55.0%
TCCTCCCACTGGCACAGAAA+TGG - chr4_1:10123624-10123643 None:intergenic 55.0%
TTTCTGTGCCAGTGGGAGGA+TGG + chr4_1:10123624-10123643 Msa0534610:CDS 55.0%
TTCTGTGCCAGTGGGAGGAT+GGG + chr4_1:10123625-10123644 Msa0534610:CDS 55.0%
ATAACAGCCCATCCTCCCAC+TGG - chr4_1:10123635-10123654 None:intergenic 55.0%
! CTTGCCATGGGGGTTAGCAA+TGG + chr4_1:10123809-10123828 Msa0534610:CDS 55.0%
! GGAAGATTAGCTGGTGGAGG+AGG - chr4_1:10123876-10123895 None:intergenic 55.0%
TCAGCGATTGTGCCGTCCAC+AGG + chr4_1:10119500-10119519 Msa0534610:CDS 60.0%
CACCATGGACAGTAGCGCGT+AGG - chr4_1:10120987-10121006 None:intergenic 60.0%
GGGTATGAGCGACCTAGTCG+TGG - chr4_1:10121024-10121043 None:intergenic 60.0%
GACATTGTGGTGCTTGCGCC+TGG + chr4_1:10122332-10122351 Msa0534610:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 10118650 10124115 10118650 ID=Msa0534610;Name=Msa0534610
chr4_1 mRNA 10118650 10124115 10118650 ID=Msa0534610-mRNA-1;Parent=Msa0534610;Name=Msa0534610-mRNA-1;_AED=0.09;_eAED=0.09;_QI=19|1|1|1|1|1|7|210|572
chr4_1 exon 10118650 10118761 10118650 ID=Msa0534610-mRNA-1:exon:3257;Parent=Msa0534610-mRNA-1
chr4_1 exon 10119483 10119634 10119483 ID=Msa0534610-mRNA-1:exon:3258;Parent=Msa0534610-mRNA-1
chr4_1 exon 10120837 10121081 10120837 ID=Msa0534610-mRNA-1:exon:3259;Parent=Msa0534610-mRNA-1
chr4_1 exon 10121443 10121571 10121443 ID=Msa0534610-mRNA-1:exon:3260;Parent=Msa0534610-mRNA-1
chr4_1 exon 10122183 10122572 10122183 ID=Msa0534610-mRNA-1:exon:3261;Parent=Msa0534610-mRNA-1
chr4_1 exon 10123099 10123677 10123099 ID=Msa0534610-mRNA-1:exon:3262;Parent=Msa0534610-mRNA-1
chr4_1 exon 10123775 10124115 10123775 ID=Msa0534610-mRNA-1:exon:3263;Parent=Msa0534610-mRNA-1
chr4_1 five_prime_UTR 10118650 10118668 10118650 ID=Msa0534610-mRNA-1:five_prime_utr;Parent=Msa0534610-mRNA-1
chr4_1 CDS 10118669 10118761 10118669 ID=Msa0534610-mRNA-1:cds;Parent=Msa0534610-mRNA-1
chr4_1 CDS 10119483 10119634 10119483 ID=Msa0534610-mRNA-1:cds;Parent=Msa0534610-mRNA-1
chr4_1 CDS 10120837 10121081 10120837 ID=Msa0534610-mRNA-1:cds;Parent=Msa0534610-mRNA-1
chr4_1 CDS 10121443 10121571 10121443 ID=Msa0534610-mRNA-1:cds;Parent=Msa0534610-mRNA-1
chr4_1 CDS 10122183 10122572 10122183 ID=Msa0534610-mRNA-1:cds;Parent=Msa0534610-mRNA-1
chr4_1 CDS 10123099 10123677 10123099 ID=Msa0534610-mRNA-1:cds;Parent=Msa0534610-mRNA-1
chr4_1 CDS 10123775 10123905 10123775 ID=Msa0534610-mRNA-1:cds;Parent=Msa0534610-mRNA-1
chr4_1 three_prime_UTR 10123906 10124115 10123906 ID=Msa0534610-mRNA-1:three_prime_utr;Parent=Msa0534610-mRNA-1
Gene Sequence

>Msa0534610

ATGACACATTTTATGTTTTCTCTAGCATGGCCTTTTGCTCTTCTATTAGCTTCTTCATTTGCTTCTGCTGCTATCGTGGAACATACTTTCAATGTTGAAGACTTCACTATTCAGCGATTGTGCCGTCCACAGGTTATCACTGCCGTCAATGGAACCTTACCAGGACCAACAATTAATGCTCGAGAGGGTGACACTGTTATCATTCATGCATTCAACAAATCACCCTACAATCTTACTCTTCATTGGCATGGAATCATTCAATTTCTAACTCCTTGGTCAGATGGTCCAGAATATGTAACCCAATGCCCAATACCATCTGGTGGCAGCTATACATACCAATTTAACTTAACTGGACAAGAGGGTACACTTTGGTGGCATGCACACTCTTCTTTCCTACGCGCTACTGTCCATGGTGCCCTTATCATTAAGCCACGACTAGGTCGCTCATACCCCTTCCCAAAGGTTTATCAAGAAGTCCCCATATTGCTTGGGGAGTGGTGGAATGCTAATGTTGAAGAAGTTGAGAGAAATGCAACAGATACGGGTAACCCACCAGCGGAGTCAAATGCATCAACAATTAATGGCTTATCTGGTGACTTGTATAACTGCTCCCAAGATCAGAATTACAAGCTCAAAGTGAAGCAAGGAAAAACCTACTTACTACGCATTATCAATGCTGCACTCAATGAACAACATTTCTTCAAGATAGCAAACCATAGTTTCACAGTTGTAGCTATGGATGCTATCTACACAGAACACTATAACACTGACATTGTGGTGCTTGCGCCTGGCCAAACCGTTGACGTTCTCTTGAAAACTAACCAAGTTATTGATTCTTATTACATGGTTATGACACCATACCGTTCTTCAAGTGTTGGAACCACTAACATAACCACTAGAGGAGTTGTTGTTTATGATGGTGCCTCCCAAACAAAAGCACCTATAATGCCAATTATGCCTGATAACCATGATACACCAACAGCTCACAAGTTTTACACCAATGTTACAGGTTTAACAACTGGTCCACATTGGGTTCCAGTTCCACGTAAAGTAGATGAACACATGTTCATAACTTTTGGTATTGGTCTAGAGCAATGCATCAATCCAGGACCAGGTCGTTGTGTTGTACTAAACTCTCGACTTTCTGCCAACATGAACAATGAATCTTTTGCTCTTCCTAGGGGTAGAGGTTTCTCAATGTTGGAAGCATTTTACAACAATATTAGTGGTGTGTACACAAAAGATTTTCCAAATCAACCACCATTTGAATTTAATTATACTGACCCTTCATTGGCTAATGTTGATCCTTCTAAGCCACTTGCATTTGCACCTAAATCAACTAAGGTGAAGACTTTGAAGTTTAATTCAACGATTGAGATTGTGCTACAAAATACAGCTATTCTTGGGGCAGAGAATCATCCTATTCATATTCATGGGTTTAACTTTCATGTTTTGGCACAAGGTTTTGGAAATTATAATGCCACTAGAGATGAACCAAAGTTTAACTTTGTTAATCCTCAAATGCGTAACACCATTTCTGTGCCAGTGGGAGGATGGGCTGTTATTAGATTTCAAGCAAATAATCCAGGTATTTGGCTTGTACATTGTCATTTGGAAACACACTTGCCATGGGGGTTAGCAATGGCTTTTGAGGTTGAGAATGGGCCTACTCTTGGAACAACAGTGCCTCCTCCACCAGCTAATCTTCCTAAGTGTTGA
Protein sequence

>Msa0534610

MTHFMFSLAWPFALLLASSFASAAIVEHTFNVEDFTIQRLCRPQVITAVNGTLPGPTINAREGDTVIIHAFNKSPYNLTLHWHGIIQFLTPWSDGPEYVTQCPIPSGGSYTYQFNLTGQEGTLWWHAHSSFLRATVHGALIIKPRLGRSYPFPKVYQEVPILLGEWWNANVEEVERNATDTGNPPAESNASTINGLSGDLYNCSQDQNYKLKVKQGKTYLLRIINAALNEQHFFKIANHSFTVVAMDAIYTEHYNTDIVVLAPGQTVDVLLKTNQVIDSYYMVMTPYRSSSVGTTNITTRGVVVYDGASQTKAPIMPIMPDNHDTPTAHKFYTNVTGLTTGPHWVPVPRKVDEHMFITFGIGLEQCINPGPGRCVVLNSRLSANMNNESFALPRGRGFSMLEAFYNNISGVYTKDFPNQPPFEFNYTDPSLANVDPSKPLAFAPKSTKVKTLKFNSTIEIVLQNTAILGAENHPIHIHGFNFHVLAQGFGNYNATRDEPKFNFVNPQMRNTISVPVGGWAVIRFQANNPGIWLVHCHLETHLPWGLAMAFEVENGPTLGTTVPPPPANLPKC*