Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0095180 | sp|Q49L15|PSBK_EUCGG | 91.228 | 57 | 5 | 0 | 3 | 59 | 5 | 61 | 5.01e-31 | 105 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0095180 | A0A0K0LTP3 | 98.246 | 57 | 1 | 0 | 3 | 59 | 5 | 61 | 1.88e-30 | 110 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0095180 | Msa0095300 | 0.842881 | 1.971985e-58 | -8.615850e-47 |
| Msa0095180 | Msa0268180 | 0.839424 | 1.598136e-57 | -8.615850e-47 |
| Msa0095180 | Msa0268380 | 0.814695 | 1.351261e-51 | -8.615850e-47 |
| Msa0095180 | Msa0268540 | 0.815960 | 7.072675e-52 | -8.615850e-47 |
| Msa0095180 | Msa0268650 | 0.809028 | 2.316595e-50 | -8.615850e-47 |
| Msa0095180 | Msa0268730 | 0.825112 | 5.597017e-54 | -8.615850e-47 |
| Msa0095180 | Msa0268850 | 0.838239 | 3.236182e-57 | -8.615850e-47 |
| Msa0095180 | Msa0268930 | 0.815561 | 8.677647e-52 | -8.615850e-47 |
| Msa0095180 | Msa0269060 | 0.824811 | 6.593800e-54 | -8.615850e-47 |
| Msa0095180 | Msa0344200 | 0.805637 | 1.212266e-49 | -8.615850e-47 |
| Msa0095180 | Msa0582530 | 0.849299 | 3.535723e-60 | -8.615850e-47 |
| Msa0095180 | Msa0582710 | 0.807071 | 6.044335e-50 | -8.615850e-47 |
| Msa0095180 | Msa0582740 | 0.808732 | 2.680616e-50 | -8.615850e-47 |
| Msa0095180 | Msa0582830 | 0.811777 | 5.908948e-51 | -8.615850e-47 |
| Msa0095180 | Msa1030280 | 0.801965 | 7.019661e-49 | -8.615850e-47 |
| Msa0095180 | Msa1086060 | 0.823607 | 1.264420e-53 | -8.615850e-47 |
| Msa0095180 | Msa1086160 | 0.804814 | 1.802997e-49 | -8.615850e-47 |
| Msa0095180 | Msa1086170 | 0.800092 | 1.694501e-48 | -8.615850e-47 |
| Msa0095180 | Msa1086290 | 0.819351 | 1.215362e-52 | -8.615850e-47 |
| Msa0095180 | Msa1086370 | 0.831351 | 1.753664e-55 | -8.615850e-47 |
| Msa0095180 | Msa1086440 | 0.802650 | 5.073761e-49 | -8.615850e-47 |
| Msa0095180 | Msa1086480 | 0.844696 | 6.444515e-59 | -8.615850e-47 |
| Msa0095180 | Msa1086870 | 0.812819 | 3.500047e-51 | -8.615850e-47 |
| Msa0095180 | Msa1091690 | 0.813723 | 2.215591e-51 | -8.615850e-47 |
| Msa0095180 | Msa1143150 | 0.829951 | 3.862167e-55 | -8.615850e-47 |
| Msa0095180 | Msa1354890 | 0.861228 | 1.188807e-63 | -8.615850e-47 |
| Msa0095180 | Msa1355060 | 0.813707 | 2.233021e-51 | -8.615850e-47 |
| Msa0095180 | Msa1394140 | 0.810495 | 1.120569e-50 | -8.615850e-47 |
| Msa0095180 | Msa1394240 | 0.827345 | 1.646726e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0095180 | MtrunA17_Chr4g0015681 | 96.491 | 57 | 2 | 0 | 3 | 59 | 5 | 61 | 1.40e-33 | 108 |
| Msa0095180 | MtrunA17_CPg0492901 | 96.491 | 57 | 2 | 0 | 3 | 59 | 5 | 61 | 1.40e-33 | 108 |
| Msa0095180 | MtrunA17_Chr4g0016771 | 96.491 | 57 | 2 | 0 | 3 | 59 | 5 | 61 | 1.40e-33 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0095180 | ATCG00070.1 | 75.439 | 57 | 14 | 0 | 3 | 59 | 5 | 61 | 1.69e-25 | 89.0 |
Find 1 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGGCATAAAATCTACAAT+TGG | 0.453871 | 1_2:-79023478 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAATTGTTACTAGAATT+TGG | + | chr1_2:79022844-79022863 | Msa0095180:intron | 15.0% |
| !! | TTGAATTGATATTTATAGAT+AGG | - | chr1_2:79023071-79023090 | None:intergenic | 15.0% |
| !! | AAAGAAAAAATGGAAAATAA+TGG | - | chr1_2:79023113-79023132 | None:intergenic | 15.0% |
| !! | ATTAAATTGTTACTAGAATT+TGG | + | chr1_2:79022844-79022863 | Msa0095180:intron | 15.0% |
| !! | TTGAATTGATATTTATAGAT+AGG | - | chr1_2:79023071-79023090 | None:intergenic | 15.0% |
| !! | AAAGAAAAAATGGAAAATAA+TGG | - | chr1_2:79023113-79023132 | None:intergenic | 15.0% |
| !! | GACACTAATAAATACTATTA+TGG | + | chr1_2:79022779-79022798 | Msa0095180:intron | 20.0% |
| !!! | CTATAGAATAAATTTAGAAC+TGG | - | chr1_2:79022819-79022838 | None:intergenic | 20.0% |
| !! | TGGATAAGTTAAAATGAAAA+AGG | + | chr1_2:79022985-79023004 | Msa0095180:intron | 20.0% |
| !! | GGATAAGTTAAAATGAAAAA+GGG | + | chr1_2:79022986-79023005 | Msa0095180:intron | 20.0% |
| !! | GAAAAAACTACTTGAATAAA+GGG | - | chr1_2:79023422-79023441 | None:intergenic | 20.0% |
| !! | AGAAAAAACTACTTGAATAA+AGG | - | chr1_2:79023423-79023442 | None:intergenic | 20.0% |
| !!! | TTATTCAAGTAGTTTTTTCT+TGG | + | chr1_2:79023423-79023442 | Msa0095180:CDS | 20.0% |
| !! | GACACTAATAAATACTATTA+TGG | + | chr1_2:79022779-79022798 | Msa0095180:intron | 20.0% |
| !!! | CTATAGAATAAATTTAGAAC+TGG | - | chr1_2:79022819-79022838 | None:intergenic | 20.0% |
| !! | TGGATAAGTTAAAATGAAAA+AGG | + | chr1_2:79022985-79023004 | Msa0095180:intron | 20.0% |
| !! | GGATAAGTTAAAATGAAAAA+GGG | + | chr1_2:79022986-79023005 | Msa0095180:intron | 20.0% |
| !! | GAAAAAACTACTTGAATAAA+GGG | - | chr1_2:79023422-79023441 | None:intergenic | 20.0% |
| !! | AGAAAAAACTACTTGAATAA+AGG | - | chr1_2:79023423-79023442 | None:intergenic | 20.0% |
| !!! | TTATTCAAGTAGTTTTTTCT+TGG | + | chr1_2:79023423-79023442 | Msa0095180:CDS | 20.0% |
| ! | GGTTATATTCAAATAGGTAT+AGG | + | chr1_2:79022651-79022670 | Msa0095180:intron | 25.0% |
| ! | TATCATGAATAGATATGATC+TGG | + | chr1_2:79022678-79022697 | Msa0095180:intron | 25.0% |
| ! | ATCATGAATAGATATGATCT+GGG | + | chr1_2:79022679-79022698 | Msa0095180:intron | 25.0% |
| ! | AGTTAAAATGAAAAAGGGAT+TGG | + | chr1_2:79022991-79023010 | Msa0095180:intron | 25.0% |
| ! | GTTAAAATGAAAAAGGGATT+GGG | + | chr1_2:79022992-79023011 | Msa0095180:intron | 25.0% |
| ! | TTAAAATGAAAAAGGGATTG+GGG | + | chr1_2:79022993-79023012 | Msa0095180:intron | 25.0% |
| ! | TATGCTTAATATCTTTAGCT+TGG | + | chr1_2:79023375-79023394 | Msa0095180:intron | 25.0% |
| !!! | TTTTTCTCTTAGCTTTTGTT+TGG | + | chr1_2:79023515-79023534 | Msa0095180:CDS | 25.0% |
| ! | GGTTATATTCAAATAGGTAT+AGG | + | chr1_2:79022651-79022670 | Msa0095180:intron | 25.0% |
| ! | TATCATGAATAGATATGATC+TGG | + | chr1_2:79022678-79022697 | Msa0095180:intron | 25.0% |
| ! | ATCATGAATAGATATGATCT+GGG | + | chr1_2:79022679-79022698 | Msa0095180:intron | 25.0% |
| ! | AGTTAAAATGAAAAAGGGAT+TGG | + | chr1_2:79022991-79023010 | Msa0095180:intron | 25.0% |
| ! | GTTAAAATGAAAAAGGGATT+GGG | + | chr1_2:79022992-79023011 | Msa0095180:intron | 25.0% |
| ! | TTAAAATGAAAAAGGGATTG+GGG | + | chr1_2:79022993-79023012 | Msa0095180:intron | 25.0% |
| ! | TATGCTTAATATCTTTAGCT+TGG | + | chr1_2:79023375-79023394 | Msa0095180:intron | 25.0% |
| !!! | TTTTTCTCTTAGCTTTTGTT+TGG | + | chr1_2:79023515-79023534 | Msa0095180:CDS | 25.0% |
| ! | ATTAGTGTCGAATCCAAAAT+GGG | - | chr1_2:79022768-79022787 | None:intergenic | 30.0% |
| ! | TATTAGTGTCGAATCCAAAA+TGG | - | chr1_2:79022769-79022788 | None:intergenic | 30.0% |
| !! | AGAATGAAACTGTTTTGAAC+TGG | + | chr1_2:79022965-79022984 | Msa0095180:intron | 30.0% |
| ! | AATTGGATTCAAAAAAGCGT+AGG | - | chr1_2:79023464-79023483 | None:intergenic | 30.0% |
| ACTGGCATAAAATCTACAAT+TGG | - | chr1_2:79023481-79023500 | None:intergenic | 30.0% | |
| !! | AAAAAGAGTAAGGGTATTAC+TGG | - | chr1_2:79023499-79023518 | None:intergenic | 30.0% |
| GCTAAGAGAAAAAAGAGTAA+GGG | - | chr1_2:79023508-79023527 | None:intergenic | 30.0% | |
| AGCTAAGAGAAAAAAGAGTA+AGG | - | chr1_2:79023509-79023528 | None:intergenic | 30.0% | |
| ! | ATTAGTGTCGAATCCAAAAT+GGG | - | chr1_2:79022768-79022787 | None:intergenic | 30.0% |
| ! | TATTAGTGTCGAATCCAAAA+TGG | - | chr1_2:79022769-79022788 | None:intergenic | 30.0% |
| !! | AGAATGAAACTGTTTTGAAC+TGG | + | chr1_2:79022965-79022984 | Msa0095180:intron | 30.0% |
| ! | AATTGGATTCAAAAAAGCGT+AGG | - | chr1_2:79023464-79023483 | None:intergenic | 30.0% |
| ACTGGCATAAAATCTACAAT+TGG | - | chr1_2:79023481-79023500 | None:intergenic | 30.0% | |
| !! | AAAAAGAGTAAGGGTATTAC+TGG | - | chr1_2:79023499-79023518 | None:intergenic | 30.0% |
| GCTAAGAGAAAAAAGAGTAA+GGG | - | chr1_2:79023508-79023527 | None:intergenic | 30.0% | |
| AGCTAAGAGAAAAAAGAGTA+AGG | - | chr1_2:79023509-79023528 | None:intergenic | 30.0% | |
| TGAATAGATATGATCTGGGA+CGG | + | chr1_2:79022683-79022702 | Msa0095180:intron | 35.0% | |
| ! | TTAGTGTCGAATCCAAAATG+GGG | - | chr1_2:79022767-79022786 | None:intergenic | 35.0% |
| TGAATAGATATGATCTGGGA+CGG | + | chr1_2:79022683-79022702 | Msa0095180:intron | 35.0% | |
| ! | TTAGTGTCGAATCCAAAATG+GGG | - | chr1_2:79022767-79022786 | None:intergenic | 35.0% |
| ! | GAATAGATATGATCTGGGAC+GGG | + | chr1_2:79022684-79022703 | Msa0095180:intron | 40.0% |
| ! | TTCAAAAAAGCGTAGGCTTC+AGG | - | chr1_2:79023457-79023476 | None:intergenic | 40.0% |
| ! | GAATAGATATGATCTGGGAC+GGG | + | chr1_2:79022684-79022703 | Msa0095180:intron | 40.0% |
| ! | TTCAAAAAAGCGTAGGCTTC+AGG | - | chr1_2:79023457-79023476 | None:intergenic | 40.0% |
| GGATTCGAACCTCCGAATAA+CGG | + | chr1_2:79022708-79022727 | Msa0095180:intron | 45.0% | |
| GATTCGAACCTCCGAATAAC+GGG | + | chr1_2:79022709-79022728 | Msa0095180:intron | 45.0% | |
| GGATTCGAACCTCCGAATAA+CGG | + | chr1_2:79022708-79022727 | Msa0095180:intron | 45.0% | |
| GATTCGAACCTCCGAATAAC+GGG | + | chr1_2:79022709-79022728 | Msa0095180:intron | 45.0% | |
| !! | AATAATTTAAAAAGAAAAAA+TGG | - | chr1_2:79023123-79023142 | None:intergenic | 5.0% |
| !! | AATAATTTAAAAAGAAAAAA+TGG | - | chr1_2:79023123-79023142 | None:intergenic | 5.0% |
| ! | TAGATATGATCTGGGACGGG+AGG | + | chr1_2:79022687-79022706 | Msa0095180:intron | 50.0% |
| !! | GTTTTGGTCCCGTTATTCGG+AGG | - | chr1_2:79022720-79022739 | None:intergenic | 50.0% |
| !! | CGGGTTTTGGTCCCGTTATT+CGG | - | chr1_2:79022723-79022742 | None:intergenic | 50.0% |
| ! | TAGATATGATCTGGGACGGG+AGG | + | chr1_2:79022687-79022706 | Msa0095180:intron | 50.0% |
| !! | GTTTTGGTCCCGTTATTCGG+AGG | - | chr1_2:79022720-79022739 | None:intergenic | 50.0% |
| !! | CGGGTTTTGGTCCCGTTATT+CGG | - | chr1_2:79022723-79022742 | None:intergenic | 50.0% |
| !! | AGCGGTAAGGCAACGGGTTT+TGG | - | chr1_2:79022736-79022755 | None:intergenic | 55.0% |
| AAACCCGTTGCCTTACCGCT+TGG | + | chr1_2:79022736-79022755 | Msa0095180:intron | 55.0% | |
| GTCGAATCCAAAATGGGGCG+TGG | - | chr1_2:79022762-79022781 | None:intergenic | 55.0% | |
| !! | AGCGGTAAGGCAACGGGTTT+TGG | - | chr1_2:79022736-79022755 | None:intergenic | 55.0% |
| AAACCCGTTGCCTTACCGCT+TGG | + | chr1_2:79022736-79022755 | Msa0095180:intron | 55.0% | |
| GTCGAATCCAAAATGGGGCG+TGG | - | chr1_2:79022762-79022781 | None:intergenic | 55.0% | |
| TGGCCAAGCGGTAAGGCAAC+GGG | - | chr1_2:79022742-79022761 | None:intergenic | 60.0% | |
| GTGGCCAAGCGGTAAGGCAA+CGG | - | chr1_2:79022743-79022762 | None:intergenic | 60.0% | |
| CAAAATGGGGCGTGGCCAAG+CGG | - | chr1_2:79022754-79022773 | None:intergenic | 60.0% | |
| TGGCCAAGCGGTAAGGCAAC+GGG | - | chr1_2:79022742-79022761 | None:intergenic | 60.0% | |
| GTGGCCAAGCGGTAAGGCAA+CGG | - | chr1_2:79022743-79022762 | None:intergenic | 60.0% | |
| CAAAATGGGGCGTGGCCAAG+CGG | - | chr1_2:79022754-79022773 | None:intergenic | 60.0% | |
| ! | CGCTTGGCCACGCCCCATTT+TGG | + | chr1_2:79022752-79022771 | Msa0095180:intron | 65.0% |
| ! | CGCTTGGCCACGCCCCATTT+TGG | + | chr1_2:79022752-79022771 | Msa0095180:intron | 65.0% |
| TGGGGCGTGGCCAAGCGGTA+AGG | - | chr1_2:79022749-79022768 | None:intergenic | 70.0% | |
| TGGGGCGTGGCCAAGCGGTA+AGG | - | chr1_2:79022749-79022768 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 79022647 | 79023558 | 79022647 | ID=Msa0095180;Name=Msa0095180 |
| chr1_2 | mRNA | 79022647 | 79023558 | 79022647 | ID=Msa0095180-mRNA-1;Parent=Msa0095180;Name=Msa0095180-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|0.66|0|0|3|0|59 |
| chr1_2 | exon | 79022647 | 79022651 | 79022647 | ID=Msa0095180-mRNA-1:exon:28084;Parent=Msa0095180-mRNA-1 |
| chr1_2 | exon | 79022667 | 79022672 | 79022667 | ID=Msa0095180-mRNA-1:exon:28085;Parent=Msa0095180-mRNA-1 |
| chr1_2 | exon | 79023393 | 79023558 | 79023393 | ID=Msa0095180-mRNA-1:exon:28086;Parent=Msa0095180-mRNA-1 |
| chr1_2 | CDS | 79022647 | 79022651 | 79022647 | ID=Msa0095180-mRNA-1:cds;Parent=Msa0095180-mRNA-1 |
| chr1_2 | CDS | 79022667 | 79022672 | 79022667 | ID=Msa0095180-mRNA-1:cds;Parent=Msa0095180-mRNA-1 |
| chr1_2 | CDS | 79023393 | 79023558 | 79023393 | ID=Msa0095180-mRNA-1:cds;Parent=Msa0095180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
