AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268380 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268380 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268380 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268380 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa0268380 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 2_2:+70209943 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 2_2:-70210046 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 2_2:+70210593 Msa0268380:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 2_2:+70210581 Msa0268380:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 2_2:-70210599 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 2_2:-70210586 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + chr2_2:70210233-70210252 Msa0268380:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - chr2_2:70210239-70210258 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - chr2_2:70210390-70210409 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - chr2_2:70210231-70210250 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + chr2_2:70210485-70210504 Msa0268380:intron 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - chr2_2:70209990-70210009 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - chr2_2:70209991-70210010 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + chr2_2:70209991-70210010 Msa0268380:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + chr2_2:70210277-70210296 Msa0268380:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + chr2_2:70210328-70210347 Msa0268380:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + chr2_2:70210419-70210438 Msa0268380:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + chr2_2:70210083-70210102 Msa0268380:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + chr2_2:70210560-70210579 Msa0268380:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - chr2_2:70210032-70210051 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - chr2_2:70210049-70210068 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - chr2_2:70210067-70210086 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - chr2_2:70210076-70210095 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - chr2_2:70210077-70210096 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - chr2_2:70210309-70210328 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - chr2_2:70210312-70210331 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + chr2_2:70210310-70210329 Msa0268380:intron 35.0%
CATATCCACAATCAAGATCT+AGG - chr2_2:70210318-70210337 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + chr2_2:70210462-70210481 Msa0268380:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - chr2_2:70210025-70210044 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + chr2_2:70210581-70210600 Msa0268380:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - chr2_2:70210589-70210608 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - chr2_2:70210306-70210325 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + chr2_2:70210593-70210612 Msa0268380:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - chr2_2:70210154-70210173 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - chr2_2:70210602-70210621 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70209944 70210628 70209944 ID=Msa0268380;Name=Msa0268380
chr2_2 mRNA 70209944 70210628 70209944 ID=Msa0268380-mRNA-1;Parent=Msa0268380;Name=Msa0268380-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|0|0|2|0|97
chr2_2 exon 70209944 70210118 70209944 ID=Msa0268380-mRNA-1:exon:22548;Parent=Msa0268380-mRNA-1
chr2_2 exon 70210510 70210628 70210510 ID=Msa0268380-mRNA-1:exon:22549;Parent=Msa0268380-mRNA-1
chr2_2 CDS 70209944 70210118 70209944 ID=Msa0268380-mRNA-1:cds;Parent=Msa0268380-mRNA-1
chr2_2 CDS 70210510 70210628 70210510 ID=Msa0268380-mRNA-1:cds;Parent=Msa0268380-mRNA-1
Gene Sequence

>Msa0268380

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa0268380

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*