Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394140 | RDY14692.1 | 90.722 | 97 | 9 | 0 | 1 | 97 | 4 | 100 | 7.75e-56 | 178 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394140 | sp|Q49L15|PSBK_EUCGG | 91.667 | 60 | 5 | 0 | 1 | 60 | 1 | 60 | 1.80e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394140 | A0A371II05 | 90.722 | 97 | 9 | 0 | 1 | 97 | 4 | 100 | 3.70e-56 | 178 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0092750 | Msa1394140 | 0.806652 | 7.412921e-50 | -8.615850e-47 |
| Msa0095140 | Msa1394140 | 0.836861 | 7.300766e-57 | -8.615850e-47 |
| Msa0095180 | Msa1394140 | 0.810495 | 1.120569e-50 | -8.615850e-47 |
| Msa0095200 | Msa1394140 | 0.828673 | 7.887228e-55 | -8.615850e-47 |
| Msa0095240 | Msa1394140 | 0.800165 | 1.638246e-48 | -8.615850e-47 |
| Msa0095270 | Msa1394140 | 0.823121 | 1.642390e-53 | -8.615850e-47 |
| Msa1354850 | Msa1394140 | 0.818043 | 2.407913e-52 | -8.615850e-47 |
| Msa1354890 | Msa1394140 | 0.885810 | 5.897513e-72 | -8.615850e-47 |
| Msa1355020 | Msa1394140 | 0.811993 | 5.302894e-51 | -8.615850e-47 |
| Msa1355060 | Msa1394140 | 0.902288 | 1.130115e-78 | -8.615850e-47 |
| Msa1365210 | Msa1394140 | 0.808990 | 2.360485e-50 | -8.615850e-47 |
| Msa1380540 | Msa1394140 | 0.819255 | 1.278608e-52 | -8.615850e-47 |
| Msa1380580 | Msa1394140 | 0.806926 | 6.487254e-50 | -8.615850e-47 |
| Msa1394050 | Msa1394140 | 0.824889 | 6.318625e-54 | -8.615850e-47 |
| Msa1394090 | Msa1394140 | 0.818425 | 1.973945e-52 | -8.615850e-47 |
| Msa1394100 | Msa1394140 | 0.820767 | 5.763722e-53 | -8.615850e-47 |
| Msa1394140 | Msa1394170 | 0.825055 | 5.775042e-54 | -8.615850e-47 |
| Msa1394140 | Msa1394240 | 0.850306 | 1.849484e-60 | -8.615850e-47 |
| Msa1394140 | Msa1394280 | 0.801529 | 8.624266e-49 | -8.615850e-47 |
| Msa1394140 | Msa1406230 | 0.804226 | 2.391517e-49 | -8.615850e-47 |
| Msa0727020 | Msa1394140 | 0.812843 | 3.457580e-51 | -8.615850e-47 |
| Msa0766880 | Msa1394140 | 0.824384 | 8.309272e-54 | -8.615850e-47 |
| Msa0772210 | Msa1394140 | 0.818363 | 2.038866e-52 | -8.615850e-47 |
| Msa0139740 | Msa1394140 | 0.804399 | 2.200769e-49 | -8.615850e-47 |
| Msa0175900 | Msa1394140 | 0.809160 | 2.170636e-50 | -8.615850e-47 |
| Msa0221860 | Msa1394140 | 0.801863 | 7.367494e-49 | -8.615850e-47 |
| Msa0222070 | Msa1394140 | 0.815241 | 1.022585e-51 | -8.615850e-47 |
| Msa0222120 | Msa1394140 | 0.814622 | 1.402967e-51 | -8.615850e-47 |
| Msa0227290 | Msa1394140 | 0.806554 | 7.777034e-50 | -8.615850e-47 |
| Msa1298740 | Msa1394140 | 0.803015 | 4.264275e-49 | -8.615850e-47 |
| Msa1327820 | Msa1394140 | 0.801661 | 8.105555e-49 | -8.615850e-47 |
| Msa0365150 | Msa1394140 | 0.823556 | 1.300072e-53 | -8.615850e-47 |
| Msa0426860 | Msa1394140 | 0.808323 | 3.276692e-50 | -8.615850e-47 |
| Msa0426890 | Msa1394140 | 0.801999 | 6.906620e-49 | -8.615850e-47 |
| Msa0427050 | Msa1394140 | 0.809456 | 1.875164e-50 | -8.615850e-47 |
| Msa0983670 | Msa1394140 | 0.801987 | 6.947642e-49 | -8.615850e-47 |
| Msa0983710 | Msa1394140 | 0.849655 | 2.812611e-60 | -8.615850e-47 |
| Msa0992570 | Msa1394140 | 0.806856 | 6.713714e-50 | -8.615850e-47 |
| Msa0992590 | Msa1394140 | 0.803764 | 2.983087e-49 | -8.615850e-47 |
| Msa1002040 | Msa1394140 | 0.802682 | 4.995683e-49 | -8.615850e-47 |
| Msa1024580 | Msa1394140 | 0.815728 | 7.968716e-52 | -8.615850e-47 |
| Msa1030280 | Msa1394140 | 0.816709 | 4.809627e-52 | -8.615850e-47 |
| Msa1070900 | Msa1394140 | 0.806382 | 8.452000e-50 | -8.615850e-47 |
| Msa1086020 | Msa1394140 | 0.805234 | 1.472756e-49 | -8.615850e-47 |
| Msa1086060 | Msa1394140 | 0.898380 | 5.614813e-77 | -8.615850e-47 |
| Msa1086160 | Msa1394140 | 0.852435 | 4.625389e-61 | -8.615850e-47 |
| Msa1086170 | Msa1394140 | 0.884882 | 1.310957e-71 | -8.615850e-47 |
| Msa1086260 | Msa1394140 | 0.823845 | 1.112253e-53 | -8.615850e-47 |
| Msa1086290 | Msa1394140 | 0.852612 | 4.117775e-61 | -8.615850e-47 |
| Msa1086370 | Msa1394140 | 0.892185 | 2.004471e-74 | -8.615850e-47 |
| Msa1086440 | Msa1394140 | 0.868383 | 6.801825e-66 | -8.615850e-47 |
| Msa1086480 | Msa1394140 | 0.853763 | 1.926601e-61 | -8.615850e-47 |
| Msa1086630 | Msa1394140 | 0.805522 | 1.282120e-49 | -8.615850e-47 |
| Msa1086690 | Msa1394140 | 0.807246 | 5.550570e-50 | -8.615850e-47 |
| Msa1086830 | Msa1394140 | 0.811391 | 7.168460e-51 | -8.615850e-47 |
| Msa1086870 | Msa1394140 | 0.871822 | 5.097313e-67 | -8.615850e-47 |
| Msa1090230 | Msa1394140 | 0.801904 | 7.223865e-49 | -8.615850e-47 |
| Msa1091690 | Msa1394140 | 0.833730 | 4.508405e-56 | -8.615850e-47 |
| Msa0253410 | Msa1394140 | 0.802184 | 6.329071e-49 | -8.615850e-47 |
| Msa0263620 | Msa1394140 | 0.817218 | 3.696105e-52 | -8.615850e-47 |
| Msa0268180 | Msa1394140 | 0.877138 | 7.990169e-69 | -8.615850e-47 |
| Msa0268220 | Msa1394140 | 0.801834 | 7.467610e-49 | -8.615850e-47 |
| Msa0268340 | Msa1394140 | 0.814990 | 1.162435e-51 | -8.615850e-47 |
| Msa0268380 | Msa1394140 | 0.888217 | 7.178387e-73 | -8.615850e-47 |
| Msa0268420 | Msa1394140 | 0.875828 | 2.265099e-68 | -8.615850e-47 |
| Msa0268460 | Msa1394140 | 0.824966 | 6.059757e-54 | -8.615850e-47 |
| Msa0268500 | Msa1394140 | 0.813221 | 2.857173e-51 | -8.615850e-47 |
| Msa0268540 | Msa1394140 | 0.889171 | 3.075442e-73 | -8.615850e-47 |
| Msa0268650 | Msa1394140 | 0.861858 | 7.632858e-64 | -8.615850e-47 |
| Msa0268730 | Msa1394140 | 0.895774 | 6.958816e-76 | -8.615850e-47 |
| Msa0268760 | Msa1394140 | 0.802973 | 4.351758e-49 | -8.615850e-47 |
| Msa0268850 | Msa1394140 | 0.877866 | 4.454650e-69 | -8.615850e-47 |
| Msa0268900 | Msa1394140 | 0.804985 | 1.660595e-49 | -8.615850e-47 |
| Msa0268930 | Msa1394140 | 0.892845 | 1.090395e-74 | -8.615850e-47 |
| Msa0269060 | Msa1394140 | 0.876303 | 1.554233e-68 | -8.615850e-47 |
| Msa0269100 | Msa1394140 | 0.810102 | 1.362076e-50 | -8.615850e-47 |
| Msa0344200 | Msa1394140 | 0.815091 | 1.104147e-51 | -8.615850e-47 |
| Msa0344250 | Msa1394140 | 0.803293 | 3.735838e-49 | -8.615850e-47 |
| Msa0344290 | Msa1394140 | 0.812046 | 5.161905e-51 | -8.615850e-47 |
| Msa1120540 | Msa1394140 | 0.819910 | 9.060816e-53 | -8.615850e-47 |
| Msa1143140 | Msa1394140 | 0.828541 | 8.491424e-55 | -8.615850e-47 |
| Msa1143150 | Msa1394140 | 0.835505 | 1.613880e-56 | -8.615850e-47 |
| Msa0582530 | Msa1394140 | 0.841409 | 4.836897e-58 | -8.615850e-47 |
| Msa0582570 | Msa1394140 | 0.802021 | 6.835589e-49 | -8.615850e-47 |
| Msa0582580 | Msa1394140 | 0.844389 | 7.794261e-59 | -8.615850e-47 |
| Msa0582620 | Msa1394140 | 0.849953 | 2.321774e-60 | -8.615850e-47 |
| Msa0582710 | Msa1394140 | 0.832124 | 1.130706e-55 | -8.615850e-47 |
| Msa0582740 | Msa1394140 | 0.882227 | 1.242618e-70 | -8.615850e-47 |
| Msa0582830 | Msa1394140 | 0.819644 | 1.042472e-52 | -8.615850e-47 |
| Msa0582900 | Msa1394140 | 0.843276 | 1.548158e-58 | -8.615850e-47 |
| Msa0591370 | Msa1394140 | 0.827367 | 1.626666e-54 | -8.615850e-47 |
| Msa0855490 | Msa1394140 | 0.800405 | 1.463578e-48 | -8.615850e-47 |
| Msa0924860 | Msa1394140 | 0.853715 | 1.989291e-61 | -8.615850e-47 |
| Msa0924890 | Msa1394140 | 0.805515 | 1.286445e-49 | -8.615850e-47 |
| Msa0931090 | Msa1394140 | 0.854976 | 8.593551e-62 | -8.615850e-47 |
| Msa0931150 | Msa1394140 | 0.825065 | 5.743285e-54 | -8.615850e-47 |
| Msa0931200 | Msa1394140 | 0.854613 | 1.095517e-61 | -8.615850e-47 |
| Msa0931280 | Msa1394140 | 0.807077 | 6.026713e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394140 | MtrunA17_Chr4g0015681 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 7.64e-35 | 113 |
| Msa1394140 | MtrunA17_CPg0492901 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 7.64e-35 | 113 |
| Msa1394140 | MtrunA17_Chr4g0016771 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 7.64e-35 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394140 | ATCG00070.1 | 76.667 | 60 | 14 | 0 | 1 | 60 | 1 | 60 | 3.76e-26 | 92.0 |
| Msa1394140 | ATCG00080.1 | 97.222 | 36 | 1 | 0 | 62 | 97 | 1 | 36 | 2.92e-19 | 73.9 |
Find 6 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCTTAATATCTTTAGCT+TGG | 0.440922 | tig0021785:+65572 | None:intergenic |
| ACTGGCATAAAATCTACAAT+TGG | 0.453871 | tig0021785:-65675 | None:intergenic |
| AATGATCCAGGACGTAATCC+TGG | 0.474800 | tig0021785:+66222 | Msa1394140:CDS |
| GGATTCCTATCTAATGATCC+AGG | 0.536202 | tig0021785:+66210 | Msa1394140:CDS |
| TCACGTCCAGGATTACGTCC+TGG | 0.548081 | tig0021785:-66228 | None:intergenic |
| TACGTCCTGGATCATTAGAT+AGG | 0.581605 | tig0021785:-66215 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCCAAATATTAAAATTTT+TGG | + | tig0021785:65862-65881 | Msa1394140:intron | 10.0% |
| !!! | TATCCAAAAATTTTAATATT+TGG | - | tig0021785:65868-65887 | None:intergenic | 10.0% |
| !! | ATTTCAAAAAAAAAAAACTT+AGG | - | tig0021785:66019-66038 | None:intergenic | 10.0% |
| !!! | AATTTTAATATTTGGAATCA+AGG | - | tig0021785:65860-65879 | None:intergenic | 15.0% |
| !!! | CGTTTTTTTTTTAATTATCT+TGG | + | tig0021785:66114-66133 | Msa1394140:intron | 15.0% |
| !! | GAAAAAACTACTTGAATAAA+GGG | - | tig0021785:65619-65638 | None:intergenic | 20.0% |
| !! | AGAAAAAACTACTTGAATAA+AGG | - | tig0021785:65620-65639 | None:intergenic | 20.0% |
| !!! | TTATTCAAGTAGTTTTTTCT+TGG | + | tig0021785:65620-65639 | Msa1394140:CDS | 20.0% |
| !! | TGAAAATTCTATTATTCGAT+TGG | + | tig0021785:65906-65925 | Msa1394140:intron | 20.0% |
| !!! | TGTGGATATGAAAAAAATTT+TGG | + | tig0021785:65957-65976 | Msa1394140:intron | 20.0% |
| !! | TCTTATAAACTTAGTATCAA+AGG | + | tig0021785:66048-66067 | Msa1394140:intron | 20.0% |
| !!! | TTTTTCTCTTAGCTTTTGTT+TGG | + | tig0021785:65712-65731 | Msa1394140:CDS | 25.0% |
| !!! | TTTGTTTCTCTTTTCATCTT+CGG | + | tig0021785:66189-66208 | Msa1394140:CDS | 25.0% |
| ! | AATTGGATTCAAAAAAGCGT+AGG | - | tig0021785:65661-65680 | None:intergenic | 30.0% |
| ACTGGCATAAAATCTACAAT+TGG | - | tig0021785:65678-65697 | None:intergenic | 30.0% | |
| !! | AAAAAGAGTAAGGGTATTAC+TGG | - | tig0021785:65696-65715 | None:intergenic | 30.0% |
| GCTAAGAGAAAAAAGAGTAA+GGG | - | tig0021785:65705-65724 | None:intergenic | 30.0% | |
| AGCTAAGAGAAAAAAGAGTA+AGG | - | tig0021785:65706-65725 | None:intergenic | 30.0% | |
| !! | AATCAAGATCTAGGTATTGG+TGG | - | tig0021785:65938-65957 | None:intergenic | 35.0% |
| CACAATCAAGATCTAGGTAT+TGG | - | tig0021785:65941-65960 | None:intergenic | 35.0% | |
| CAATACCTAGATCTTGATTG+TGG | + | tig0021785:65939-65958 | Msa1394140:intron | 35.0% | |
| CATATCCACAATCAAGATCT+AGG | - | tig0021785:65947-65966 | None:intergenic | 35.0% | |
| GAGAATCTATTCTCTTTCTC+TGG | + | tig0021785:66091-66110 | Msa1394140:intron | 35.0% | |
| ! | TTCAAAAAAGCGTAGGCTTC+AGG | - | tig0021785:65654-65673 | None:intergenic | 40.0% |
| GGATTCCTATCTAATGATCC+AGG | + | tig0021785:66210-66229 | Msa1394140:CDS | 40.0% | |
| TACGTCCTGGATCATTAGAT+AGG | - | tig0021785:66218-66237 | None:intergenic | 40.0% | |
| !! | CAAGATCTAGGTATTGGTGG+TGG | - | tig0021785:65935-65954 | None:intergenic | 45.0% |
| AATGATCCAGGACGTAATCC+TGG | + | tig0021785:66222-66241 | Msa1394140:CDS | 45.0% | |
| !!! | AATAAATTCTTAATTTTTTT+CGG | - | tig0021785:65783-65802 | None:intergenic | 5.0% |
| TCACGTCCAGGATTACGTCC+TGG | - | tig0021785:66231-66250 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021785 | gene | 65573 | 66257 | 65573 | ID=Msa1394140;Name=Msa1394140 |
| tig0021785 | mRNA | 65573 | 66257 | 65573 | ID=Msa1394140-mRNA-1;Parent=Msa1394140;Name=Msa1394140-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97 |
| tig0021785 | exon | 65573 | 65747 | 65573 | ID=Msa1394140-mRNA-1:exon:14250;Parent=Msa1394140-mRNA-1 |
| tig0021785 | exon | 66139 | 66257 | 66139 | ID=Msa1394140-mRNA-1:exon:14251;Parent=Msa1394140-mRNA-1 |
| tig0021785 | CDS | 65573 | 65747 | 65573 | ID=Msa1394140-mRNA-1:cds;Parent=Msa1394140-mRNA-1 |
| tig0021785 | CDS | 66139 | 66257 | 66139 | ID=Msa1394140-mRNA-1:cds;Parent=Msa1394140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
