Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394240 | RDY14692.1 | 90.722 | 97 | 9 | 0 | 1 | 97 | 4 | 100 | 7.75e-56 | 178 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394240 | sp|Q49L15|PSBK_EUCGG | 91.667 | 60 | 5 | 0 | 1 | 60 | 1 | 60 | 1.80e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394240 | A0A371II05 | 90.722 | 97 | 9 | 0 | 1 | 97 | 4 | 100 | 3.70e-56 | 178 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0035090 | Msa1394240 | 0.809799 | 1.582643e-50 | -8.615850e-47 |
| Msa0094420 | Msa1394240 | 0.801291 | 9.649274e-49 | -8.615850e-47 |
| Msa0095140 | Msa1394240 | 0.828277 | 9.833310e-55 | -8.615850e-47 |
| Msa0095180 | Msa1394240 | 0.827345 | 1.646726e-54 | -8.615850e-47 |
| Msa0095200 | Msa1394240 | 0.842180 | 3.027080e-58 | -8.615850e-47 |
| Msa0095240 | Msa1394240 | 0.801530 | 8.623352e-49 | -8.615850e-47 |
| Msa0095270 | Msa1394240 | 0.825900 | 3.643416e-54 | -8.615850e-47 |
| Msa0095300 | Msa1394240 | 0.813376 | 2.640974e-51 | -8.615850e-47 |
| Msa1354850 | Msa1394240 | 0.817905 | 2.587039e-52 | -8.615850e-47 |
| Msa1354890 | Msa1394240 | 0.907943 | 2.932676e-81 | -8.615850e-47 |
| Msa1355020 | Msa1394240 | 0.808354 | 3.227934e-50 | -8.615850e-47 |
| Msa1355060 | Msa1394240 | 0.852131 | 5.645967e-61 | -8.615850e-47 |
| Msa1367940 | Msa1394240 | 0.800125 | 1.668911e-48 | -8.615850e-47 |
| Msa1380540 | Msa1394240 | 0.803425 | 3.507409e-49 | -8.615850e-47 |
| Msa1380580 | Msa1394240 | 0.805116 | 1.559393e-49 | -8.615850e-47 |
| Msa1387090 | Msa1394240 | 0.811173 | 7.993555e-51 | -8.615850e-47 |
| Msa1394050 | Msa1394240 | 0.865532 | 5.515126e-65 | -8.615850e-47 |
| Msa1394090 | Msa1394240 | 0.810936 | 8.995703e-51 | -8.615850e-47 |
| Msa1394100 | Msa1394240 | 0.802905 | 4.494605e-49 | -8.615850e-47 |
| Msa1394140 | Msa1394240 | 0.850306 | 1.849484e-60 | -8.615850e-47 |
| Msa0702070 | Msa1394240 | 0.800565 | 1.357755e-48 | -8.615850e-47 |
| Msa0727020 | Msa1394240 | 0.811949 | 5.419097e-51 | -8.615850e-47 |
| Msa0766880 | Msa1394240 | 0.809747 | 1.624135e-50 | -8.615850e-47 |
| Msa0772210 | Msa1394240 | 0.809338 | 1.988080e-50 | -8.615850e-47 |
| Msa0782330 | Msa1394240 | 0.806953 | 6.403259e-50 | -8.615850e-47 |
| Msa0134550 | Msa1394240 | 0.803165 | 3.970671e-49 | -8.615850e-47 |
| Msa0139740 | Msa1394240 | 0.804353 | 2.249571e-49 | -8.615850e-47 |
| Msa0175900 | Msa1394240 | 0.811431 | 7.026460e-51 | -8.615850e-47 |
| Msa0221860 | Msa1394240 | 0.810487 | 1.124679e-50 | -8.615850e-47 |
| Msa0222070 | Msa1394240 | 0.808929 | 2.432020e-50 | -8.615850e-47 |
| Msa0222120 | Msa1394240 | 0.808019 | 3.803372e-50 | -8.615850e-47 |
| Msa0224960 | Msa1394240 | 0.800876 | 1.173263e-48 | -8.615850e-47 |
| Msa0227290 | Msa1394240 | 0.800653 | 1.302597e-48 | -8.615850e-47 |
| Msa1284030 | Msa1394240 | 0.810438 | 1.152733e-50 | -8.615850e-47 |
| Msa1298740 | Msa1394240 | 0.805684 | 1.185023e-49 | -8.615850e-47 |
| Msa1309910 | Msa1394240 | 0.809880 | 1.520107e-50 | -8.615850e-47 |
| Msa1323060 | Msa1394240 | 0.804685 | 1.918476e-49 | -8.615850e-47 |
| Msa1327820 | Msa1394240 | 0.806808 | 6.873250e-50 | -8.615850e-47 |
| Msa0365150 | Msa1394240 | 0.800185 | 1.622612e-48 | -8.615850e-47 |
| Msa0427050 | Msa1394240 | 0.805187 | 1.506626e-49 | -8.615850e-47 |
| Msa0983710 | Msa1394240 | 0.855721 | 5.214951e-62 | -8.615850e-47 |
| Msa0992570 | Msa1394240 | 0.808016 | 3.809421e-50 | -8.615850e-47 |
| Msa0992590 | Msa1394240 | 0.821767 | 3.389781e-53 | -8.615850e-47 |
| Msa1002040 | Msa1394240 | 0.828672 | 7.893791e-55 | -8.615850e-47 |
| Msa1030280 | Msa1394240 | 0.829311 | 5.527114e-55 | -8.615850e-47 |
| Msa1070900 | Msa1394240 | 0.825857 | 3.728339e-54 | -8.615850e-47 |
| Msa1086020 | Msa1394240 | 0.814654 | 1.380184e-51 | -8.615850e-47 |
| Msa1086060 | Msa1394240 | 0.863141 | 3.075532e-64 | -8.615850e-47 |
| Msa1086160 | Msa1394240 | 0.851588 | 8.046759e-61 | -8.615850e-47 |
| Msa1086170 | Msa1394240 | 0.903759 | 2.487866e-79 | -8.615850e-47 |
| Msa1086290 | Msa1394240 | 0.879426 | 1.258567e-69 | -8.615850e-47 |
| Msa1086340 | Msa1394240 | 0.812162 | 4.870750e-51 | -8.615850e-47 |
| Msa1086370 | Msa1394240 | 0.856677 | 2.737695e-62 | -8.615850e-47 |
| Msa1086440 | Msa1394240 | 0.830953 | 2.196332e-55 | -8.615850e-47 |
| Msa1086480 | Msa1394240 | 0.883310 | 5.001380e-71 | -8.615850e-47 |
| Msa1086630 | Msa1394240 | 0.803027 | 4.241123e-49 | -8.615850e-47 |
| Msa1086830 | Msa1394240 | 0.803732 | 3.029546e-49 | -8.615850e-47 |
| Msa1086870 | Msa1394240 | 0.846227 | 2.479192e-59 | -8.615850e-47 |
| Msa1091690 | Msa1394240 | 0.845715 | 3.415777e-59 | -8.615850e-47 |
| Msa0263620 | Msa1394240 | 0.808559 | 2.917929e-50 | -8.615850e-47 |
| Msa0268180 | Msa1394240 | 0.899281 | 2.314354e-77 | -8.615850e-47 |
| Msa0268340 | Msa1394240 | 0.805536 | 1.273374e-49 | -8.615850e-47 |
| Msa0268380 | Msa1394240 | 0.883809 | 3.276519e-71 | -8.615850e-47 |
| Msa0268420 | Msa1394240 | 0.886512 | 3.207604e-72 | -8.615850e-47 |
| Msa0268500 | Msa1394240 | 0.804696 | 1.908303e-49 | -8.615850e-47 |
| Msa0268540 | Msa1394240 | 0.887150 | 1.836738e-72 | -8.615850e-47 |
| Msa0268650 | Msa1394240 | 0.892772 | 1.166101e-74 | -8.615850e-47 |
| Msa0268730 | Msa1394240 | 0.860279 | 2.306826e-63 | -8.615850e-47 |
| Msa0268850 | Msa1394240 | 0.911484 | 5.770703e-83 | -8.615850e-47 |
| Msa0268930 | Msa1394240 | 0.876567 | 1.260083e-68 | -8.615850e-47 |
| Msa0269060 | Msa1394240 | 0.901888 | 1.698616e-78 | -8.615850e-47 |
| Msa0269100 | Msa1394240 | 0.800527 | 1.382156e-48 | -8.615850e-47 |
| Msa0306130 | Msa1394240 | 0.810959 | 8.890620e-51 | -8.615850e-47 |
| Msa0344200 | Msa1394240 | 0.843052 | 1.776351e-58 | -8.615850e-47 |
| Msa1143140 | Msa1394240 | 0.865899 | 4.223522e-65 | -8.615850e-47 |
| Msa1143150 | Msa1394240 | 0.831128 | 1.989514e-55 | -8.615850e-47 |
| Msa1157160 | Msa1394240 | 0.804084 | 2.559672e-49 | -8.615850e-47 |
| Msa1195860 | Msa1394240 | 0.806479 | 8.064348e-50 | -8.615850e-47 |
| Msa0540340 | Msa1394240 | 0.811753 | 5.979740e-51 | -8.615850e-47 |
| Msa0558890 | Msa1394240 | 0.818323 | 2.081058e-52 | -8.615850e-47 |
| Msa0582530 | Msa1394240 | 0.898452 | 5.231961e-77 | -8.615850e-47 |
| Msa0582580 | Msa1394240 | 0.807679 | 4.493356e-50 | -8.615850e-47 |
| Msa0582620 | Msa1394240 | 0.849214 | 3.733847e-60 | -8.615850e-47 |
| Msa0582710 | Msa1394240 | 0.902580 | 8.384621e-79 | -8.615850e-47 |
| Msa0582740 | Msa1394240 | 0.865923 | 4.152248e-65 | -8.615850e-47 |
| Msa0582830 | Msa1394240 | 0.891070 | 5.556319e-74 | -8.615850e-47 |
| Msa0582900 | Msa1394240 | 0.849664 | 2.796057e-60 | -8.615850e-47 |
| Msa0586890 | Msa1394240 | 0.800253 | 1.571427e-48 | -8.615850e-47 |
| Msa0591390 | Msa1394240 | 0.803415 | 3.523777e-49 | -8.615850e-47 |
| Msa0595780 | Msa1394240 | 0.802755 | 4.826527e-49 | -8.615850e-47 |
| Msa0860270 | Msa1394240 | 0.801278 | 9.710489e-49 | -8.615850e-47 |
| Msa0924860 | Msa1394240 | 0.851711 | 7.426929e-61 | -8.615850e-47 |
| Msa0931090 | Msa1394240 | 0.901286 | 3.125122e-78 | -8.615850e-47 |
| Msa0931150 | Msa1394240 | 0.874996 | 4.361080e-68 | -8.615850e-47 |
| Msa0931200 | Msa1394240 | 0.833607 | 4.839379e-56 | -8.615850e-47 |
| Msa0931280 | Msa1394240 | 0.842761 | 2.122342e-58 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394240 | MtrunA17_Chr4g0015681 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 7.64e-35 | 113 |
| Msa1394240 | MtrunA17_CPg0492901 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 7.64e-35 | 113 |
| Msa1394240 | MtrunA17_Chr4g0016771 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 7.64e-35 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394240 | ATCG00070.1 | 76.667 | 60 | 14 | 0 | 1 | 60 | 1 | 60 | 3.76e-26 | 92.0 |
| Msa1394240 | ATCG00080.1 | 97.222 | 36 | 1 | 0 | 62 | 97 | 1 | 36 | 2.92e-19 | 73.9 |
Find 6 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCTTAATATCTTTAGCT+TGG | 0.440922 | tig0021785:-89419 | Msa1394240:CDS |
| ACTGGCATAAAATCTACAAT+TGG | 0.453871 | tig0021785:+89316 | None:intergenic |
| AATGATCCAGGACGTAATCC+TGG | 0.474800 | tig0021785:-88769 | Msa1394240:CDS |
| GGATTCCTATCTAATGATCC+AGG | 0.536202 | tig0021785:-88781 | Msa1394240:CDS |
| TCACGTCCAGGATTACGTCC+TGG | 0.548081 | tig0021785:+88763 | None:intergenic |
| TACGTCCTGGATCATTAGAT+AGG | 0.581605 | tig0021785:+88776 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCCAAATATTAAAATTTT+TGG | - | tig0021785:89045-89064 | Msa1394240:intron | 10.0% |
| !!! | TATCCAAAAATTTTAATATT+TGG | + | tig0021785:89051-89070 | None:intergenic | 10.0% |
| !! | ATTTCAAAAAAAAAAAACTT+AGG | + | tig0021785:89202-89221 | None:intergenic | 10.0% |
| !!! | AATTTTAATATTTGGAATCA+AGG | + | tig0021785:89043-89062 | None:intergenic | 15.0% |
| !!! | CGTTTTTTTTTTAATTATCT+TGG | - | tig0021785:89297-89316 | Msa1394240:CDS | 15.0% |
| !! | GAAAAAACTACTTGAATAAA+GGG | + | tig0021785:88802-88821 | None:intergenic | 20.0% |
| !! | AGAAAAAACTACTTGAATAA+AGG | + | tig0021785:88803-88822 | None:intergenic | 20.0% |
| !!! | TTATTCAAGTAGTTTTTTCT+TGG | - | tig0021785:88803-88822 | Msa1394240:CDS | 20.0% |
| !! | TGAAAATTCTATTATTCGAT+TGG | - | tig0021785:89089-89108 | Msa1394240:intron | 20.0% |
| !!! | TGTGGATATGAAAAAAATTT+TGG | - | tig0021785:89140-89159 | Msa1394240:intron | 20.0% |
| !! | TCTTATAAACTTAGTATCAA+AGG | - | tig0021785:89231-89250 | Msa1394240:intron | 20.0% |
| !!! | TTTTTCTCTTAGCTTTTGTT+TGG | - | tig0021785:88895-88914 | Msa1394240:intron | 25.0% |
| !!! | TTTGTTTCTCTTTTCATCTT+CGG | - | tig0021785:89372-89391 | Msa1394240:CDS | 25.0% |
| ! | AATTGGATTCAAAAAAGCGT+AGG | + | tig0021785:88844-88863 | None:intergenic | 30.0% |
| ACTGGCATAAAATCTACAAT+TGG | + | tig0021785:88861-88880 | None:intergenic | 30.0% | |
| !! | AAAAAGAGTAAGGGTATTAC+TGG | + | tig0021785:88879-88898 | None:intergenic | 30.0% |
| GCTAAGAGAAAAAAGAGTAA+GGG | + | tig0021785:88888-88907 | None:intergenic | 30.0% | |
| AGCTAAGAGAAAAAAGAGTA+AGG | + | tig0021785:88889-88908 | None:intergenic | 30.0% | |
| !! | AATCAAGATCTAGGTATTGG+TGG | + | tig0021785:89121-89140 | None:intergenic | 35.0% |
| CACAATCAAGATCTAGGTAT+TGG | + | tig0021785:89124-89143 | None:intergenic | 35.0% | |
| CAATACCTAGATCTTGATTG+TGG | - | tig0021785:89122-89141 | Msa1394240:intron | 35.0% | |
| CATATCCACAATCAAGATCT+AGG | + | tig0021785:89130-89149 | None:intergenic | 35.0% | |
| GAGAATCTATTCTCTTTCTC+TGG | - | tig0021785:89274-89293 | Msa1394240:CDS | 35.0% | |
| ! | TTCAAAAAAGCGTAGGCTTC+AGG | + | tig0021785:88837-88856 | None:intergenic | 40.0% |
| GGATTCCTATCTAATGATCC+AGG | - | tig0021785:89393-89412 | Msa1394240:CDS | 40.0% | |
| TACGTCCTGGATCATTAGAT+AGG | + | tig0021785:89401-89420 | None:intergenic | 40.0% | |
| !! | CAAGATCTAGGTATTGGTGG+TGG | + | tig0021785:89118-89137 | None:intergenic | 45.0% |
| AATGATCCAGGACGTAATCC+TGG | - | tig0021785:89405-89424 | Msa1394240:CDS | 45.0% | |
| !!! | AATAAATTCTTAATTTTTTT+CGG | + | tig0021785:88966-88985 | None:intergenic | 5.0% |
| TCACGTCCAGGATTACGTCC+TGG | + | tig0021785:89414-89433 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021785 | gene | 88756 | 89440 | 88756 | ID=Msa1394240;Name=Msa1394240 |
| tig0021785 | mRNA | 88756 | 89440 | 88756 | ID=Msa1394240-mRNA-1;Parent=Msa1394240;Name=Msa1394240-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97 |
| tig0021785 | exon | 89266 | 89440 | 89266 | ID=Msa1394240-mRNA-1:exon:14255;Parent=Msa1394240-mRNA-1 |
| tig0021785 | exon | 88756 | 88874 | 88756 | ID=Msa1394240-mRNA-1:exon:14254;Parent=Msa1394240-mRNA-1 |
| tig0021785 | CDS | 89266 | 89440 | 89266 | ID=Msa1394240-mRNA-1:cds;Parent=Msa1394240-mRNA-1 |
| tig0021785 | CDS | 88756 | 88874 | 88756 | ID=Msa1394240-mRNA-1:cds;Parent=Msa1394240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
