Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1086480 | RDY14692.1 | 90.722 | 97 | 9 | 0 | 1 | 97 | 4 | 100 | 7.75e-56 | 178 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1086480 | sp|Q49L15|PSBK_EUCGG | 91.667 | 60 | 5 | 0 | 1 | 60 | 1 | 60 | 1.80e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1086480 | A0A371II05 | 90.722 | 97 | 9 | 0 | 1 | 97 | 4 | 100 | 3.70e-56 | 178 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0035090 | Msa1086480 | 0.810325 | 1.219312e-50 | -8.615850e-47 |
| Msa0092750 | Msa1086480 | 0.803277 | 3.764444e-49 | -8.615850e-47 |
| Msa0095140 | Msa1086480 | 0.854484 | 1.193421e-61 | -8.615850e-47 |
| Msa0095180 | Msa1086480 | 0.844696 | 6.444515e-59 | -8.615850e-47 |
| Msa0095200 | Msa1086480 | 0.835684 | 1.453941e-56 | -8.615850e-47 |
| Msa0095240 | Msa1086480 | 0.812766 | 3.593723e-51 | -8.615850e-47 |
| Msa0095270 | Msa1086480 | 0.853085 | 3.017108e-61 | -8.615850e-47 |
| Msa0647110 | Msa1086480 | 0.804832 | 1.787648e-49 | -8.615850e-47 |
| Msa0727020 | Msa1086480 | 0.822863 | 1.886516e-53 | -8.615850e-47 |
| Msa0766880 | Msa1086480 | 0.832693 | 8.173350e-56 | -8.615850e-47 |
| Msa0804930 | Msa1086480 | 0.801853 | 7.402193e-49 | -8.615850e-47 |
| Msa0130120 | Msa1086480 | 0.802549 | 5.322639e-49 | -8.615850e-47 |
| Msa0139740 | Msa1086480 | 0.813592 | 2.367219e-51 | -8.615850e-47 |
| Msa0156780 | Msa1086480 | 0.810940 | 8.975420e-51 | -8.615850e-47 |
| Msa0175900 | Msa1086480 | 0.810593 | 1.067151e-50 | -8.615850e-47 |
| Msa0221860 | Msa1086480 | 0.839454 | 1.569023e-57 | -8.615850e-47 |
| Msa0426860 | Msa1086480 | 0.803788 | 2.949422e-49 | -8.615850e-47 |
| Msa0427050 | Msa1086480 | 0.800091 | 1.695292e-48 | -8.615850e-47 |
| Msa0449930 | Msa1086480 | 0.810798 | 9.634171e-51 | -8.615850e-47 |
| Msa0983670 | Msa1086480 | 0.817511 | 3.175219e-52 | -8.615850e-47 |
| Msa0983710 | Msa1086480 | 0.809816 | 1.569353e-50 | -8.615850e-47 |
| Msa0992590 | Msa1086480 | 0.809148 | 2.183410e-50 | -8.615850e-47 |
| Msa1030280 | Msa1086480 | 0.808243 | 3.408208e-50 | -8.615850e-47 |
| Msa1086020 | Msa1086480 | 0.835713 | 1.429507e-56 | -8.615850e-47 |
| Msa1086060 | Msa1086480 | 0.866150 | 3.519576e-65 | -8.615850e-47 |
| Msa1086160 | Msa1086480 | 0.884363 | 2.044306e-71 | -8.615850e-47 |
| Msa1086170 | Msa1086480 | 0.889556 | 2.179653e-73 | -8.615850e-47 |
| Msa1086260 | Msa1086480 | 0.813938 | 1.986814e-51 | -8.615850e-47 |
| Msa1086290 | Msa1086480 | 0.911153 | 8.395387e-83 | -8.615850e-47 |
| Msa1086330 | Msa1086480 | 0.824070 | 9.851343e-54 | -8.615850e-47 |
| Msa1086340 | Msa1086480 | 0.810555 | 1.087768e-50 | -8.615850e-47 |
| Msa1086370 | Msa1086480 | 0.891099 | 5.408391e-74 | -8.615850e-47 |
| Msa1086440 | Msa1086480 | 0.847515 | 1.101512e-59 | -8.615850e-47 |
| Msa1086480 | Msa1086520 | 0.816914 | 4.325449e-52 | -8.615850e-47 |
| Msa1086480 | Msa1086630 | 0.842322 | 2.774711e-58 | -8.615850e-47 |
| Msa1086480 | Msa1086690 | 0.815826 | 7.576231e-52 | -8.615850e-47 |
| Msa1086480 | Msa1086830 | 0.835884 | 1.293935e-56 | -8.615850e-47 |
| Msa1086480 | Msa1086870 | 0.868078 | 8.525718e-66 | -8.615850e-47 |
| Msa1086480 | Msa1090230 | 0.809836 | 1.554067e-50 | -8.615850e-47 |
| Msa1086480 | Msa1091690 | 0.863239 | 2.868134e-64 | -8.615850e-47 |
| Msa1086480 | Msa1143140 | 0.858372 | 8.618663e-63 | -8.615850e-47 |
| Msa1086480 | Msa1143150 | 0.815437 | 9.247421e-52 | -8.615850e-47 |
| Msa1086480 | Msa1157160 | 0.804974 | 1.669312e-49 | -8.615850e-47 |
| Msa1086480 | Msa1195860 | 0.811478 | 6.861698e-51 | -8.615850e-47 |
| Msa1086480 | Msa1248400 | 0.810594 | 1.066542e-50 | -8.615850e-47 |
| Msa1086480 | Msa1298740 | 0.801530 | 8.621193e-49 | -8.615850e-47 |
| Msa1086480 | Msa1327820 | 0.802757 | 4.822565e-49 | -8.615850e-47 |
| Msa1086480 | Msa1342030 | 0.813686 | 2.257188e-51 | -8.615850e-47 |
| Msa1086480 | Msa1354850 | 0.857556 | 1.505965e-62 | -8.615850e-47 |
| Msa1086480 | Msa1354890 | 0.874286 | 7.606198e-68 | -8.615850e-47 |
| Msa1086480 | Msa1355020 | 0.842733 | 2.158922e-58 | -8.615850e-47 |
| Msa1086480 | Msa1355060 | 0.864142 | 1.503968e-64 | -8.615850e-47 |
| Msa1086480 | Msa1380540 | 0.840784 | 7.056863e-58 | -8.615850e-47 |
| Msa1086480 | Msa1385940 | 0.813395 | 2.616372e-51 | -8.615850e-47 |
| Msa1086480 | Msa1394090 | 0.856686 | 2.719739e-62 | -8.615850e-47 |
| Msa1086480 | Msa1394100 | 0.828145 | 1.058082e-54 | -8.615850e-47 |
| Msa1086480 | Msa1394140 | 0.853763 | 1.926601e-61 | -8.615850e-47 |
| Msa1086480 | Msa1394240 | 0.883310 | 5.001380e-71 | -8.615850e-47 |
| Msa1086480 | Msa1394280 | 0.843342 | 1.486173e-58 | -8.615850e-47 |
| Msa1086480 | Msa1426870 | 0.802057 | 6.719158e-49 | -8.615850e-47 |
| Msa0253410 | Msa1086480 | 0.802356 | 5.831730e-49 | -8.615850e-47 |
| Msa0268180 | Msa1086480 | 0.900441 | 7.302950e-78 | -8.615850e-47 |
| Msa0268220 | Msa1086480 | 0.828267 | 9.886715e-55 | -8.615850e-47 |
| Msa0268340 | Msa1086480 | 0.835973 | 1.228336e-56 | -8.615850e-47 |
| Msa0268380 | Msa1086480 | 0.862323 | 5.498306e-64 | -8.615850e-47 |
| Msa0268420 | Msa1086480 | 0.909285 | 6.740601e-82 | -8.615850e-47 |
| Msa0268460 | Msa1086480 | 0.824304 | 8.677022e-54 | -8.615850e-47 |
| Msa0268500 | Msa1086480 | 0.833428 | 5.364571e-56 | -8.615850e-47 |
| Msa0268540 | Msa1086480 | 0.887994 | 8.744068e-73 | -8.615850e-47 |
| Msa0268650 | Msa1086480 | 0.909056 | 8.682229e-82 | -8.615850e-47 |
| Msa0268730 | Msa1086480 | 0.852627 | 4.076487e-61 | -8.615850e-47 |
| Msa0268760 | Msa1086480 | 0.800936 | 1.140833e-48 | -8.615850e-47 |
| Msa0268850 | Msa1086480 | 0.923135 | 3.957427e-89 | -8.615850e-47 |
| Msa0268900 | Msa1086480 | 0.817066 | 3.997885e-52 | -8.615850e-47 |
| Msa0268930 | Msa1086480 | 0.854609 | 1.098648e-61 | -8.615850e-47 |
| Msa0269060 | Msa1086480 | 0.906880 | 9.240463e-81 | -8.615850e-47 |
| Msa0269100 | Msa1086480 | 0.824151 | 9.427547e-54 | -8.615850e-47 |
| Msa0324060 | Msa1086480 | 0.802375 | 5.780957e-49 | -8.615850e-47 |
| Msa0344200 | Msa1086480 | 0.855334 | 6.765273e-62 | -8.615850e-47 |
| Msa0344250 | Msa1086480 | 0.837118 | 6.277867e-57 | -8.615850e-47 |
| Msa0487560 | Msa1086480 | 0.800670 | 1.292715e-48 | -8.615850e-47 |
| Msa0582530 | Msa1086480 | 0.880683 | 4.484621e-70 | -8.615850e-47 |
| Msa0582570 | Msa1086480 | 0.831501 | 1.610253e-55 | -8.615850e-47 |
| Msa0582580 | Msa1086480 | 0.842754 | 2.131176e-58 | -8.615850e-47 |
| Msa0582620 | Msa1086480 | 0.863085 | 3.202313e-64 | -8.615850e-47 |
| Msa0582710 | Msa1086480 | 0.893021 | 9.259599e-75 | -8.615850e-47 |
| Msa0582740 | Msa1086480 | 0.859141 | 5.077645e-63 | -8.615850e-47 |
| Msa0582830 | Msa1086480 | 0.893655 | 5.133094e-75 | -8.615850e-47 |
| Msa0582900 | Msa1086480 | 0.857716 | 1.350096e-62 | -8.615850e-47 |
| Msa0591370 | Msa1086480 | 0.808976 | 2.376688e-50 | -8.615850e-47 |
| Msa0924820 | Msa1086480 | 0.811242 | 7.721930e-51 | -8.615850e-47 |
| Msa0924860 | Msa1086480 | 0.834710 | 2.560262e-56 | -8.615850e-47 |
| Msa0924890 | Msa1086480 | 0.818066 | 2.380048e-52 | -8.615850e-47 |
| Msa0925020 | Msa1086480 | 0.825958 | 3.528275e-54 | -8.615850e-47 |
| Msa0931090 | Msa1086480 | 0.887540 | 1.304744e-72 | -8.615850e-47 |
| Msa0931150 | Msa1086480 | 0.891634 | 3.321708e-74 | -8.615850e-47 |
| Msa0931200 | Msa1086480 | 0.812875 | 3.402490e-51 | -8.615850e-47 |
| Msa0931280 | Msa1086480 | 0.887592 | 1.246248e-72 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1086480 | MtrunA17_Chr4g0015681 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 7.64e-35 | 113 |
| Msa1086480 | MtrunA17_CPg0492901 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 7.64e-35 | 113 |
| Msa1086480 | MtrunA17_Chr4g0016771 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 7.64e-35 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1086480 | ATCG00070.1 | 76.667 | 60 | 14 | 0 | 1 | 60 | 1 | 60 | 3.76e-26 | 92.0 |
| Msa1086480 | ATCG00080.1 | 97.222 | 36 | 1 | 0 | 62 | 97 | 1 | 36 | 2.92e-19 | 73.9 |
Find 6 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCTTAATATCTTTAGCT+TGG | 0.440922 | 7_2:-87414577 | Msa1086480:CDS |
| ACTGGCATAAAATCTACAAT+TGG | 0.453871 | 7_2:+87414474 | None:intergenic |
| AATGATCCAGGACGTAATCC+TGG | 0.474800 | 7_2:-87413929 | Msa1086480:CDS |
| GGATTCCTATCTAATGATCC+AGG | 0.536202 | 7_2:-87413941 | Msa1086480:CDS |
| TCACGTCCAGGATTACGTCC+TGG | 0.548081 | 7_2:+87413923 | None:intergenic |
| TACGTCCTGGATCATTAGAT+AGG | 0.581605 | 7_2:+87413936 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCCAAATATTAAAATTTT+TGG | - | chr7_2:87414205-87414224 | Msa1086480:intron | 10.0% |
| !!! | TATCCAAAAATTTTAATATT+TGG | + | chr7_2:87414211-87414230 | None:intergenic | 10.0% |
| !! | AATTTCAAAAAAAAAAACTT+AGG | + | chr7_2:87414362-87414381 | None:intergenic | 10.0% |
| !!! | AATTTTAATATTTGGAATCA+AGG | + | chr7_2:87414203-87414222 | None:intergenic | 15.0% |
| !! | GAAAAAACTACTTGAATAAA+GGG | + | chr7_2:87413962-87413981 | None:intergenic | 20.0% |
| !! | AGAAAAAACTACTTGAATAA+AGG | + | chr7_2:87413963-87413982 | None:intergenic | 20.0% |
| !!! | TTATTCAAGTAGTTTTTTCT+TGG | - | chr7_2:87413963-87413982 | Msa1086480:CDS | 20.0% |
| !! | TGAAAATTCTATTATTCGAT+TGG | - | chr7_2:87414249-87414268 | Msa1086480:intron | 20.0% |
| !!! | TGTGGATATGAAAAAAATTT+TGG | - | chr7_2:87414300-87414319 | Msa1086480:intron | 20.0% |
| !! | TCTTATAAACTTAGTATCAA+AGG | - | chr7_2:87414390-87414409 | Msa1086480:intron | 20.0% |
| !!! | GCGTTTTTTTTTAATTATCT+TGG | - | chr7_2:87414455-87414474 | Msa1086480:CDS | 20.0% |
| !!! | TTTTTCTCTTAGCTTTTGTT+TGG | - | chr7_2:87414055-87414074 | Msa1086480:intron | 25.0% |
| !!! | TTTGTTTCTCTTTTCATCTT+CGG | - | chr7_2:87414530-87414549 | Msa1086480:CDS | 25.0% |
| ! | AATTGGATTCAAAAAAGCGT+AGG | + | chr7_2:87414004-87414023 | None:intergenic | 30.0% |
| ACTGGCATAAAATCTACAAT+TGG | + | chr7_2:87414021-87414040 | None:intergenic | 30.0% | |
| !! | AAAAAGAGTAAGGGTATTAC+TGG | + | chr7_2:87414039-87414058 | None:intergenic | 30.0% |
| GCTAAGAGAAAAAAGAGTAA+GGG | + | chr7_2:87414048-87414067 | None:intergenic | 30.0% | |
| AGCTAAGAGAAAAAAGAGTA+AGG | + | chr7_2:87414049-87414068 | None:intergenic | 30.0% | |
| !! | AATCAAGATCTAGGTATTGG+TGG | + | chr7_2:87414281-87414300 | None:intergenic | 35.0% |
| CACAATCAAGATCTAGGTAT+TGG | + | chr7_2:87414284-87414303 | None:intergenic | 35.0% | |
| CAATACCTAGATCTTGATTG+TGG | - | chr7_2:87414282-87414301 | Msa1086480:intron | 35.0% | |
| CATATCCACAATCAAGATCT+AGG | + | chr7_2:87414290-87414309 | None:intergenic | 35.0% | |
| GAGAATCTATTCTCTTTCTC+TGG | - | chr7_2:87414433-87414452 | Msa1086480:CDS | 35.0% | |
| ! | TTCAAAAAAGCGTAGGCTTC+AGG | + | chr7_2:87413997-87414016 | None:intergenic | 40.0% |
| GGATTCCTATCTAATGATCC+AGG | - | chr7_2:87414551-87414570 | Msa1086480:CDS | 40.0% | |
| TACGTCCTGGATCATTAGAT+AGG | + | chr7_2:87414559-87414578 | None:intergenic | 40.0% | |
| !! | CAAGATCTAGGTATTGGTGG+TGG | + | chr7_2:87414278-87414297 | None:intergenic | 45.0% |
| AATGATCCAGGACGTAATCC+TGG | - | chr7_2:87414563-87414582 | Msa1086480:CDS | 45.0% | |
| !!! | AATAAATTCTTAATTTTTTT+CGG | + | chr7_2:87414126-87414145 | None:intergenic | 5.0% |
| TCACGTCCAGGATTACGTCC+TGG | + | chr7_2:87414572-87414591 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 87413916 | 87414598 | 87413916 | ID=Msa1086480;Name=Msa1086480 |
| chr7_2 | mRNA | 87413916 | 87414598 | 87413916 | ID=Msa1086480-mRNA-1;Parent=Msa1086480;Name=Msa1086480-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97 |
| chr7_2 | exon | 87414424 | 87414598 | 87414424 | ID=Msa1086480-mRNA-1:exon:23846;Parent=Msa1086480-mRNA-1 |
| chr7_2 | exon | 87413916 | 87414034 | 87413916 | ID=Msa1086480-mRNA-1:exon:23845;Parent=Msa1086480-mRNA-1 |
| chr7_2 | CDS | 87414424 | 87414598 | 87414424 | ID=Msa1086480-mRNA-1:cds;Parent=Msa1086480-mRNA-1 |
| chr7_2 | CDS | 87413916 | 87414034 | 87413916 | ID=Msa1086480-mRNA-1:cds;Parent=Msa1086480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
