AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268540 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268540 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268540 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268540 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa0268540 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 2_2:+70300602 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 2_2:-70300705 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 2_2:+70301252 Msa0268540:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 2_2:+70301240 Msa0268540:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 2_2:-70301258 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 2_2:-70301245 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + chr2_2:70300892-70300911 Msa0268540:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - chr2_2:70300898-70300917 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - chr2_2:70301049-70301068 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - chr2_2:70300890-70300909 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + chr2_2:70301144-70301163 Msa0268540:intron 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - chr2_2:70300649-70300668 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - chr2_2:70300650-70300669 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + chr2_2:70300650-70300669 Msa0268540:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + chr2_2:70300936-70300955 Msa0268540:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + chr2_2:70300987-70301006 Msa0268540:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + chr2_2:70301078-70301097 Msa0268540:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + chr2_2:70300742-70300761 Msa0268540:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + chr2_2:70301219-70301238 Msa0268540:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - chr2_2:70300691-70300710 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - chr2_2:70300708-70300727 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - chr2_2:70300726-70300745 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - chr2_2:70300735-70300754 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - chr2_2:70300736-70300755 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - chr2_2:70300968-70300987 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - chr2_2:70300971-70300990 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + chr2_2:70300969-70300988 Msa0268540:intron 35.0%
CATATCCACAATCAAGATCT+AGG - chr2_2:70300977-70300996 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + chr2_2:70301121-70301140 Msa0268540:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - chr2_2:70300684-70300703 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + chr2_2:70301240-70301259 Msa0268540:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - chr2_2:70301248-70301267 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - chr2_2:70300965-70300984 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + chr2_2:70301252-70301271 Msa0268540:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - chr2_2:70300813-70300832 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - chr2_2:70301261-70301280 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70300603 70301287 70300603 ID=Msa0268540;Name=Msa0268540
chr2_2 mRNA 70300603 70301287 70300603 ID=Msa0268540-mRNA-1;Parent=Msa0268540;Name=Msa0268540-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr2_2 exon 70300603 70300777 70300603 ID=Msa0268540-mRNA-1:exon:22604;Parent=Msa0268540-mRNA-1
chr2_2 exon 70301169 70301287 70301169 ID=Msa0268540-mRNA-1:exon:22605;Parent=Msa0268540-mRNA-1
chr2_2 CDS 70300603 70300777 70300603 ID=Msa0268540-mRNA-1:cds;Parent=Msa0268540-mRNA-1
chr2_2 CDS 70301169 70301287 70301169 ID=Msa0268540-mRNA-1:cds;Parent=Msa0268540-mRNA-1
Gene Sequence

>Msa0268540

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa0268540

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*