AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1086870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086870 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086870 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086870 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086870 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa1086870 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 7_2:+87603577 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 7_2:-87603680 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 7_2:+87604227 Msa1086870:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 7_2:+87604215 Msa1086870:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 7_2:-87604233 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 7_2:-87604220 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + chr7_2:87603867-87603886 Msa1086870:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - chr7_2:87603873-87603892 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - chr7_2:87604024-87604043 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - chr7_2:87603865-87603884 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + chr7_2:87604119-87604138 Msa1086870:intron 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - chr7_2:87603624-87603643 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - chr7_2:87603625-87603644 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + chr7_2:87603625-87603644 Msa1086870:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + chr7_2:87603911-87603930 Msa1086870:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + chr7_2:87603962-87603981 Msa1086870:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + chr7_2:87604053-87604072 Msa1086870:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + chr7_2:87603717-87603736 Msa1086870:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + chr7_2:87604194-87604213 Msa1086870:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - chr7_2:87603666-87603685 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - chr7_2:87603683-87603702 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - chr7_2:87603701-87603720 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - chr7_2:87603710-87603729 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - chr7_2:87603711-87603730 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - chr7_2:87603943-87603962 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - chr7_2:87603946-87603965 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + chr7_2:87603944-87603963 Msa1086870:intron 35.0%
CATATCCACAATCAAGATCT+AGG - chr7_2:87603952-87603971 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + chr7_2:87604096-87604115 Msa1086870:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - chr7_2:87603659-87603678 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + chr7_2:87604215-87604234 Msa1086870:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - chr7_2:87604223-87604242 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - chr7_2:87603940-87603959 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + chr7_2:87604227-87604246 Msa1086870:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - chr7_2:87603788-87603807 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - chr7_2:87604236-87604255 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87603578 87604262 87603578 ID=Msa1086870;Name=Msa1086870
chr7_2 mRNA 87603578 87604262 87603578 ID=Msa1086870-mRNA-1;Parent=Msa1086870;Name=Msa1086870-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr7_2 exon 87603578 87603752 87603578 ID=Msa1086870-mRNA-1:exon:23963;Parent=Msa1086870-mRNA-1
chr7_2 exon 87604144 87604262 87604144 ID=Msa1086870-mRNA-1:exon:23964;Parent=Msa1086870-mRNA-1
chr7_2 CDS 87603578 87603752 87603578 ID=Msa1086870-mRNA-1:cds;Parent=Msa1086870-mRNA-1
chr7_2 CDS 87604144 87604262 87604144 ID=Msa1086870-mRNA-1:cds;Parent=Msa1086870-mRNA-1
Gene Sequence

>Msa1086870

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa1086870

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*