Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1091690 | RDY14692.1 | 89.691 | 97 | 10 | 0 | 1 | 97 | 4 | 100 | 6.37e-55 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1091690 | sp|Q49L15|PSBK_EUCGG | 91.667 | 60 | 5 | 0 | 1 | 60 | 1 | 60 | 1.59e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1091690 | A0A371II05 | 89.691 | 97 | 10 | 0 | 1 | 97 | 4 | 100 | 3.04e-55 | 176 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0095140 | Msa1091690 | 0.835763 | 1.388546e-56 | -8.615850e-47 |
| Msa0095180 | Msa1091690 | 0.813723 | 2.215591e-51 | -8.615850e-47 |
| Msa0095200 | Msa1091690 | 0.844099 | 9.323970e-59 | -8.615850e-47 |
| Msa0095240 | Msa1091690 | 0.803858 | 2.852264e-49 | -8.615850e-47 |
| Msa0095270 | Msa1091690 | 0.824319 | 8.609480e-54 | -8.615850e-47 |
| Msa0766880 | Msa1091690 | 0.805238 | 1.470227e-49 | -8.615850e-47 |
| Msa0782330 | Msa1091690 | 0.801485 | 8.806664e-49 | -8.615850e-47 |
| Msa0804930 | Msa1091690 | 0.804729 | 1.877940e-49 | -8.615850e-47 |
| Msa0221860 | Msa1091690 | 0.806353 | 8.570859e-50 | -8.615850e-47 |
| Msa0224960 | Msa1091690 | 0.800249 | 1.574392e-48 | -8.615850e-47 |
| Msa0992570 | Msa1091690 | 0.806527 | 7.879186e-50 | -8.615850e-47 |
| Msa1030280 | Msa1091690 | 0.811577 | 6.529505e-51 | -8.615850e-47 |
| Msa1086020 | Msa1091690 | 0.808394 | 3.165378e-50 | -8.615850e-47 |
| Msa1086060 | Msa1091690 | 0.856945 | 2.281755e-62 | -8.615850e-47 |
| Msa1086160 | Msa1091690 | 0.850099 | 2.113010e-60 | -8.615850e-47 |
| Msa1086170 | Msa1091690 | 0.865974 | 4.001381e-65 | -8.615850e-47 |
| Msa1086290 | Msa1091690 | 0.887095 | 1.926826e-72 | -8.615850e-47 |
| Msa1086370 | Msa1091690 | 0.849214 | 3.732119e-60 | -8.615850e-47 |
| Msa1086440 | Msa1091690 | 0.861524 | 9.657729e-64 | -8.615850e-47 |
| Msa1086480 | Msa1091690 | 0.863239 | 2.868134e-64 | -8.615850e-47 |
| Msa1086630 | Msa1091690 | 0.805746 | 1.150364e-49 | -8.615850e-47 |
| Msa1086870 | Msa1091690 | 0.864270 | 1.372100e-64 | -8.615850e-47 |
| Msa1091690 | Msa1143140 | 0.841285 | 5.214227e-58 | -8.615850e-47 |
| Msa1091690 | Msa1143150 | 0.801253 | 9.822638e-49 | -8.615850e-47 |
| Msa1091690 | Msa1354850 | 0.821604 | 3.696782e-53 | -8.615850e-47 |
| Msa1091690 | Msa1354890 | 0.837745 | 4.335253e-57 | -8.615850e-47 |
| Msa1091690 | Msa1355020 | 0.807395 | 5.161181e-50 | -8.615850e-47 |
| Msa1091690 | Msa1355060 | 0.835849 | 1.320728e-56 | -8.615850e-47 |
| Msa1091690 | Msa1380540 | 0.803907 | 2.785737e-49 | -8.615850e-47 |
| Msa1091690 | Msa1394050 | 0.806394 | 8.403868e-50 | -8.615850e-47 |
| Msa1091690 | Msa1394090 | 0.812755 | 3.613163e-51 | -8.615850e-47 |
| Msa1091690 | Msa1394100 | 0.801345 | 9.409364e-49 | -8.615850e-47 |
| Msa1091690 | Msa1394140 | 0.833730 | 4.508405e-56 | -8.615850e-47 |
| Msa1091690 | Msa1394240 | 0.845715 | 3.415777e-59 | -8.615850e-47 |
| Msa1091690 | Msa1394280 | 0.806443 | 8.204551e-50 | -8.615850e-47 |
| Msa1091690 | Msa1415120 | 0.804795 | 1.819493e-49 | -8.615850e-47 |
| Msa0268180 | Msa1091690 | 0.874407 | 6.917290e-68 | -8.615850e-47 |
| Msa0268340 | Msa1091690 | 0.803737 | 3.021683e-49 | -8.615850e-47 |
| Msa0268380 | Msa1091690 | 0.845380 | 4.210288e-59 | -8.615850e-47 |
| Msa0268420 | Msa1091690 | 0.880342 | 5.940143e-70 | -8.615850e-47 |
| Msa0268500 | Msa1091690 | 0.803191 | 3.921191e-49 | -8.615850e-47 |
| Msa0268540 | Msa1091690 | 0.877635 | 5.364957e-69 | -8.615850e-47 |
| Msa0268650 | Msa1091690 | 0.886311 | 3.820176e-72 | -8.615850e-47 |
| Msa0268730 | Msa1091690 | 0.859417 | 4.196663e-63 | -8.615850e-47 |
| Msa0268850 | Msa1091690 | 0.868807 | 4.963147e-66 | -8.615850e-47 |
| Msa0268900 | Msa1091690 | 0.822573 | 2.203334e-53 | -8.615850e-47 |
| Msa0268930 | Msa1091690 | 0.846696 | 1.846939e-59 | -8.615850e-47 |
| Msa0269060 | Msa1091690 | 0.875865 | 2.198633e-68 | -8.615850e-47 |
| Msa0344200 | Msa1091690 | 0.813386 | 2.628074e-51 | -8.615850e-47 |
| Msa0344290 | Msa1091690 | 0.803130 | 4.037425e-49 | -8.615850e-47 |
| Msa0582530 | Msa1091690 | 0.832793 | 7.716876e-56 | -8.615850e-47 |
| Msa0582570 | Msa1091690 | 0.811126 | 8.180479e-51 | -8.615850e-47 |
| Msa0582580 | Msa1091690 | 0.805793 | 1.124256e-49 | -8.615850e-47 |
| Msa0582620 | Msa1091690 | 0.834398 | 3.066521e-56 | -8.615850e-47 |
| Msa0582710 | Msa1091690 | 0.861759 | 8.188288e-64 | -8.615850e-47 |
| Msa0582740 | Msa1091690 | 0.846676 | 1.870745e-59 | -8.615850e-47 |
| Msa0582830 | Msa1091690 | 0.857209 | 1.907917e-62 | -8.615850e-47 |
| Msa0582900 | Msa1091690 | 0.847372 | 1.205867e-59 | -8.615850e-47 |
| Msa0924860 | Msa1091690 | 0.803048 | 4.197249e-49 | -8.615850e-47 |
| Msa0925020 | Msa1091690 | 0.803357 | 3.623258e-49 | -8.615850e-47 |
| Msa0931090 | Msa1091690 | 0.835969 | 1.231328e-56 | -8.615850e-47 |
| Msa0931150 | Msa1091690 | 0.855431 | 6.336976e-62 | -8.615850e-47 |
| Msa0931200 | Msa1091690 | 0.824484 | 7.872475e-54 | -8.615850e-47 |
| Msa0931280 | Msa1091690 | 0.835401 | 1.714494e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1091690 | MtrunA17_Chr4g0015681 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 6.63e-35 | 114 |
| Msa1091690 | MtrunA17_CPg0492901 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 6.63e-35 | 114 |
| Msa1091690 | MtrunA17_Chr4g0016771 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 6.63e-35 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1091690 | ATCG00070.1 | 76.667 | 60 | 14 | 0 | 1 | 60 | 1 | 60 | 3.64e-26 | 92.0 |
| Msa1091690 | ATCG00080.1 | 97.143 | 35 | 1 | 0 | 63 | 97 | 2 | 36 | 2.02e-18 | 72.0 |
Find 6 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCTTAATATCTTTAGCT+TGG | 0.440922 | 7_3:-7150125 | Msa1091690:CDS |
| ACTGGCATAAAATCTACAAT+TGG | 0.453871 | 7_3:+7150022 | None:intergenic |
| AATGATCCAGGACGTAATCC+TGG | 0.474800 | 7_3:-7149474 | Msa1091690:CDS |
| GGATTCCTATCTAATGATCC+AGG | 0.536202 | 7_3:-7149486 | Msa1091690:CDS |
| TCACGTCCAGGATTACGTCC+TGG | 0.548081 | 7_3:+7149468 | None:intergenic |
| TACGTCCTGGATCATTAGAT+AGG | 0.581605 | 7_3:+7149481 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCCAAATATTAAAATTTT+TGG | - | chr7_3:7149750-7149769 | Msa1091690:intron | 10.0% |
| !!! | TATCCAAAAATTTTAATATT+TGG | + | chr7_3:7149756-7149775 | None:intergenic | 10.0% |
| !! | ATTTCAAAAAAAAAAAACTT+AGG | + | chr7_3:7149907-7149926 | None:intergenic | 10.0% |
| !!! | AATTTTAATATTTGGAATCA+AGG | + | chr7_3:7149748-7149767 | None:intergenic | 15.0% |
| !!! | GTTTCTTTTTTTAATTATCT+TGG | - | chr7_3:7150003-7150022 | Msa1091690:CDS | 15.0% |
| !! | GAAAAAACTACTTGAATAAA+GGG | + | chr7_3:7149507-7149526 | None:intergenic | 20.0% |
| !! | AGAAAAAACTACTTGAATAA+AGG | + | chr7_3:7149508-7149527 | None:intergenic | 20.0% |
| !!! | TTATTCAAGTAGTTTTTTCT+TGG | - | chr7_3:7149508-7149527 | Msa1091690:CDS | 20.0% |
| !! | TGAAAATTCTATTATTCGAT+TGG | - | chr7_3:7149794-7149813 | Msa1091690:intron | 20.0% |
| !!! | TGTGGATATGAAAAAAATTT+TGG | - | chr7_3:7149845-7149864 | Msa1091690:intron | 20.0% |
| !! | TCTTATAAACTTAGTATCAA+AGG | - | chr7_3:7149936-7149955 | Msa1091690:intron | 20.0% |
| !!! | TTTTTCTCTTAGCTTTTGTT+TGG | - | chr7_3:7149600-7149619 | Msa1091690:intron | 25.0% |
| !!! | TTTGTTTCTCTTTTCATCTT+CGG | - | chr7_3:7150078-7150097 | Msa1091690:CDS | 25.0% |
| ! | AATTGGATTCAAAAAAGCGT+AGG | + | chr7_3:7149549-7149568 | None:intergenic | 30.0% |
| ACTGGCATAAAATCTACAAT+TGG | + | chr7_3:7149566-7149585 | None:intergenic | 30.0% | |
| !! | AAAAAGAGTAAGGGTATTAC+TGG | + | chr7_3:7149584-7149603 | None:intergenic | 30.0% |
| GCTAAGAGAAAAAAGAGTAA+GGG | + | chr7_3:7149593-7149612 | None:intergenic | 30.0% | |
| AGCTAAGAGAAAAAAGAGTA+AGG | + | chr7_3:7149594-7149613 | None:intergenic | 30.0% | |
| GAGAATCTATTCTCTTTCTT+TGG | - | chr7_3:7149979-7149998 | Msa1091690:CDS | 30.0% | |
| !! | AATCAAGATCTAGGTATTGG+TGG | + | chr7_3:7149826-7149845 | None:intergenic | 35.0% |
| CACAATCAAGATCTAGGTAT+TGG | + | chr7_3:7149829-7149848 | None:intergenic | 35.0% | |
| CAATACCTAGATCTTGATTG+TGG | - | chr7_3:7149827-7149846 | Msa1091690:intron | 35.0% | |
| CATATCCACAATCAAGATCT+AGG | + | chr7_3:7149835-7149854 | None:intergenic | 35.0% | |
| ! | TTCAAAAAAGCGTAGGCTTC+AGG | + | chr7_3:7149542-7149561 | None:intergenic | 40.0% |
| GGATTCCTATCTAATGATCC+AGG | - | chr7_3:7150099-7150118 | Msa1091690:CDS | 40.0% | |
| TACGTCCTGGATCATTAGAT+AGG | + | chr7_3:7150107-7150126 | None:intergenic | 40.0% | |
| !! | CAAGATCTAGGTATTGGTGG+TGG | + | chr7_3:7149823-7149842 | None:intergenic | 45.0% |
| AATGATCCAGGACGTAATCC+TGG | - | chr7_3:7150111-7150130 | Msa1091690:CDS | 45.0% | |
| !!! | AATAAATTCTTAATTTTTTT+CGG | + | chr7_3:7149671-7149690 | None:intergenic | 5.0% |
| TCACGTCCAGGATTACGTCC+TGG | + | chr7_3:7150120-7150139 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 7149461 | 7150146 | 7149461 | ID=Msa1091690;Name=Msa1091690 |
| chr7_3 | mRNA | 7149461 | 7150146 | 7149461 | ID=Msa1091690-mRNA-1;Parent=Msa1091690;Name=Msa1091690-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|0|0|2|0|97 |
| chr7_3 | exon | 7149972 | 7150146 | 7149972 | ID=Msa1091690-mRNA-1:exon:2257;Parent=Msa1091690-mRNA-1 |
| chr7_3 | exon | 7149461 | 7149579 | 7149461 | ID=Msa1091690-mRNA-1:exon:2256;Parent=Msa1091690-mRNA-1 |
| chr7_3 | CDS | 7149972 | 7150146 | 7149972 | ID=Msa1091690-mRNA-1:cds;Parent=Msa1091690-mRNA-1 |
| chr7_3 | CDS | 7149461 | 7149579 | 7149461 | ID=Msa1091690-mRNA-1:cds;Parent=Msa1091690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
