AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1086290


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086290 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086290 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086290 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086290 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa1086290 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 7_2:-87303876 Msa1086290:CDS
ACTGGCATAAAATCTACAAT+TGG 0.453871 7_2:+87303773 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 7_2:-87303227 Msa1086290:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 7_2:-87303239 Msa1086290:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 7_2:+87303221 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 7_2:+87303234 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG - chr7_2:87303503-87303522 Msa1086290:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG + chr7_2:87303509-87303528 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG + chr7_2:87303660-87303679 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG + chr7_2:87303501-87303520 None:intergenic 15.0%
!! GAAAAAACTACTTGAATAAA+GGG + chr7_2:87303260-87303279 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG + chr7_2:87303261-87303280 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG - chr7_2:87303261-87303280 Msa1086290:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG - chr7_2:87303547-87303566 Msa1086290:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG - chr7_2:87303598-87303617 Msa1086290:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG - chr7_2:87303689-87303708 Msa1086290:intron 20.0%
!!! GCGTTTTTTTTTAATTATCT+TGG - chr7_2:87303754-87303773 Msa1086290:CDS 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG - chr7_2:87303353-87303372 Msa1086290:intron 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG - chr7_2:87303829-87303848 Msa1086290:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG + chr7_2:87303302-87303321 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG + chr7_2:87303319-87303338 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG + chr7_2:87303337-87303356 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG + chr7_2:87303346-87303365 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG + chr7_2:87303347-87303366 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG + chr7_2:87303579-87303598 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG + chr7_2:87303582-87303601 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG - chr7_2:87303580-87303599 Msa1086290:intron 35.0%
CATATCCACAATCAAGATCT+AGG + chr7_2:87303588-87303607 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG - chr7_2:87303732-87303751 Msa1086290:CDS 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG + chr7_2:87303295-87303314 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG - chr7_2:87303850-87303869 Msa1086290:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG + chr7_2:87303858-87303877 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG + chr7_2:87303576-87303595 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG - chr7_2:87303862-87303881 Msa1086290:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG + chr7_2:87303424-87303443 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG + chr7_2:87303871-87303890 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87303214 87303897 87303214 ID=Msa1086290;Name=Msa1086290
chr7_2 mRNA 87303214 87303897 87303214 ID=Msa1086290-mRNA-1;Parent=Msa1086290;Name=Msa1086290-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr7_2 exon 87303723 87303897 87303723 ID=Msa1086290-mRNA-1:exon:23782;Parent=Msa1086290-mRNA-1
chr7_2 exon 87303214 87303332 87303214 ID=Msa1086290-mRNA-1:exon:23781;Parent=Msa1086290-mRNA-1
chr7_2 CDS 87303723 87303897 87303723 ID=Msa1086290-mRNA-1:cds;Parent=Msa1086290-mRNA-1
chr7_2 CDS 87303214 87303332 87303214 ID=Msa1086290-mRNA-1:cds;Parent=Msa1086290-mRNA-1
Gene Sequence

>Msa1086290

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa1086290

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*