AlfalfaGEDB | Msa1143150

M. sativa cultivar zhongmu-4 / Msa1143150

KEGG: K02710|K02712
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF02533.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1143150	RDY14692.1	90.722	97	9	0	1	97	4	100	7.75e-56	178

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1143150	sp\|Q49L15\|PSBK_EUCGG	91.667	60	5	0	1	60	1	60	1.80e-32	110

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1143150	A0A371II05	90.722	97	9	0	1	97	4	100	3.70e-56	178

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0095140	Msa1143150	0.802291	6.015502e-49	-8.615850e-47
Msa0095180	Msa1143150	0.829951	3.862167e-55	-8.615850e-47
Msa0095270	Msa1143150	0.805840	1.099001e-49	-8.615850e-47
Msa0983710	Msa1143150	0.832879	7.347300e-56	-8.615850e-47
Msa0992570	Msa1143150	0.804011	2.650302e-49	-8.615850e-47
Msa1030280	Msa1143150	0.832873	7.373104e-56	-8.615850e-47
Msa1086060	Msa1143150	0.843966	1.012140e-58	-8.615850e-47
Msa1086160	Msa1143150	0.819641	1.043834e-52	-8.615850e-47
Msa1086170	Msa1143150	0.868849	4.807257e-66	-8.615850e-47
Msa1086290	Msa1143150	0.845074	5.094182e-59	-8.615850e-47
Msa1086370	Msa1143150	0.857140	1.999343e-62	-8.615850e-47
Msa1086440	Msa1143150	0.837659	4.561832e-57	-8.615850e-47
Msa1086480	Msa1143150	0.815437	9.247421e-52	-8.615850e-47
Msa1086870	Msa1143150	0.825076	5.710387e-54	-8.615850e-47
Msa1091690	Msa1143150	0.801253	9.822638e-49	-8.615850e-47
Msa0268180	Msa1143150	0.830407	2.988453e-55	-8.615850e-47
Msa0268380	Msa1143150	0.855992	4.348126e-62	-8.615850e-47
Msa0268420	Msa1143150	0.832352	9.926323e-56	-8.615850e-47
Msa0268540	Msa1143150	0.835686	1.451995e-56	-8.615850e-47
Msa0268650	Msa1143150	0.837336	5.521050e-57	-8.615850e-47
Msa0268730	Msa1143150	0.850317	1.835716e-60	-8.615850e-47
Msa0268850	Msa1143150	0.824834	6.510208e-54	-8.615850e-47
Msa0268930	Msa1143150	0.827436	1.566342e-54	-8.615850e-47
Msa0269060	Msa1143150	0.829262	5.680638e-55	-8.615850e-47
Msa0344290	Msa1143150	0.803836	2.881850e-49	-8.615850e-47
Msa1143140	Msa1143150	0.804016	2.644432e-49	-8.615850e-47
Msa1143150	Msa1354890	0.873418	1.493472e-67	-8.615850e-47
Msa1143150	Msa1355060	0.830483	2.863265e-55	-8.615850e-47
Msa1143150	Msa1380580	0.837625	4.653331e-57	-8.615850e-47
Msa1143150	Msa1394050	0.816759	4.686889e-52	-8.615850e-47
Msa1143150	Msa1394140	0.835505	1.613880e-56	-8.615850e-47
Msa1143150	Msa1394240	0.831128	1.989514e-55	-8.615850e-47
Msa0582530	Msa1143150	0.804424	2.174291e-49	-8.615850e-47
Msa0582620	Msa1143150	0.819801	9.595093e-53	-8.615850e-47
Msa0582740	Msa1143150	0.865988	3.958551e-65	-8.615850e-47
Msa0582830	Msa1143150	0.808936	2.424626e-50	-8.615850e-47
Msa0582900	Msa1143150	0.834527	2.846945e-56	-8.615850e-47
Msa0924860	Msa1143150	0.806652	7.411760e-50	-8.615850e-47
Msa0931090	Msa1143150	0.813430	2.569481e-51	-8.615850e-47
Msa0931150	Msa1143150	0.832573	8.749636e-56	-8.615850e-47
Msa0931200	Msa1143150	0.821930	3.107607e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1143150	MtrunA17_Chr4g0015681	96.667	60	2	1	60	1	60	7.64e-35	113
Msa1143150	MtrunA17_CPg0492901	96.667	60	2	1	60	1	60	7.64e-35	113
Msa1143150	MtrunA17_Chr4g0016771	96.667	60	2	1	60	1	60	7.64e-35	113

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1143150	ATCG00070.1	76.667	60	14	0	1	60	1	60	3.76e-26	92.0
Msa1143150	ATCG00080.1	97.222	36	1	0	62	97	1	36	2.92e-19	73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGCTTAATATCTTTAGCT+TGG	0.440922	7_4:+18353286	None:intergenic
ACTGGCATAAAATCTACAAT+TGG	0.453871	7_4:-18353389	None:intergenic
AATGATCCAGGACGTAATCC+TGG	0.474800	7_4:+18353936	Msa1143150:CDS
GGATTCCTATCTAATGATCC+AGG	0.536202	7_4:+18353924	Msa1143150:CDS
TCACGTCCAGGATTACGTCC+TGG	0.548081	7_4:-18353942	None:intergenic
TACGTCCTGGATCATTAGAT+AGG	0.581605	7_4:-18353929	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTCCAAATATTAAAATTTT+TGG	+	chr7_4:18353576-18353595	Msa1143150:intron	10.0%
!!!	TATCCAAAAATTTTAATATT+TGG	-	chr7_4:18353582-18353601	None:intergenic	10.0%
!!	ATTTCAAAAAAAAAAAACTT+AGG	-	chr7_4:18353733-18353752	None:intergenic	10.0%
!!!	AATTTTAATATTTGGAATCA+AGG	-	chr7_4:18353574-18353593	None:intergenic	15.0%
!!!	CGTTTTTTTTTTAATTATCT+TGG	+	chr7_4:18353828-18353847	Msa1143150:intron	15.0%
!!	GAAAAAACTACTTGAATAAA+GGG	-	chr7_4:18353333-18353352	None:intergenic	20.0%
!!	AGAAAAAACTACTTGAATAA+AGG	-	chr7_4:18353334-18353353	None:intergenic	20.0%
!!!	TTATTCAAGTAGTTTTTTCT+TGG	+	chr7_4:18353334-18353353	Msa1143150:CDS	20.0%
!!	TGAAAATTCTATTATTCGAT+TGG	+	chr7_4:18353620-18353639	Msa1143150:intron	20.0%
!!!	TGTGGATATGAAAAAAATTT+TGG	+	chr7_4:18353671-18353690	Msa1143150:intron	20.0%
!!	TCTTATAAACTTAGTATCAA+AGG	+	chr7_4:18353762-18353781	Msa1143150:intron	20.0%
!!!	TTTTTCTCTTAGCTTTTGTT+TGG	+	chr7_4:18353426-18353445	Msa1143150:CDS	25.0%
!!!	TTTGTTTCTCTTTTCATCTT+CGG	+	chr7_4:18353903-18353922	Msa1143150:CDS	25.0%
!	AATTGGATTCAAAAAAGCGT+AGG	-	chr7_4:18353375-18353394	None:intergenic	30.0%
	ACTGGCATAAAATCTACAAT+TGG	-	chr7_4:18353392-18353411	None:intergenic	30.0%
!!	AAAAAGAGTAAGGGTATTAC+TGG	-	chr7_4:18353410-18353429	None:intergenic	30.0%
	GCTAAGAGAAAAAAGAGTAA+GGG	-	chr7_4:18353419-18353438	None:intergenic	30.0%
	AGCTAAGAGAAAAAAGAGTA+AGG	-	chr7_4:18353420-18353439	None:intergenic	30.0%
!!	AATCAAGATCTAGGTATTGG+TGG	-	chr7_4:18353652-18353671	None:intergenic	35.0%
	CACAATCAAGATCTAGGTAT+TGG	-	chr7_4:18353655-18353674	None:intergenic	35.0%
	CAATACCTAGATCTTGATTG+TGG	+	chr7_4:18353653-18353672	Msa1143150:intron	35.0%
	CATATCCACAATCAAGATCT+AGG	-	chr7_4:18353661-18353680	None:intergenic	35.0%
	GAGAATCTATTCTCTTTCTC+TGG	+	chr7_4:18353805-18353824	Msa1143150:intron	35.0%
!	TTCAAAAAAGCGTAGGCTTC+AGG	-	chr7_4:18353368-18353387	None:intergenic	40.0%
	GGATTCCTATCTAATGATCC+AGG	+	chr7_4:18353924-18353943	Msa1143150:CDS	40.0%
	TACGTCCTGGATCATTAGAT+AGG	-	chr7_4:18353932-18353951	None:intergenic	40.0%
!!	CAAGATCTAGGTATTGGTGG+TGG	-	chr7_4:18353649-18353668	None:intergenic	45.0%
	AATGATCCAGGACGTAATCC+TGG	+	chr7_4:18353936-18353955	Msa1143150:CDS	45.0%
!!!	AATAAATTCTTAATTTTTTT+CGG	-	chr7_4:18353497-18353516	None:intergenic	5.0%
	TCACGTCCAGGATTACGTCC+TGG	-	chr7_4:18353945-18353964	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	18353287	18353971	18353287	ID=Msa1143150;Name=Msa1143150
chr7_4	mRNA	18353287	18353971	18353287	ID=Msa1143150-mRNA-1;Parent=Msa1143150;Name=Msa1143150-mRNA-1;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|97
chr7_4	exon	18353287	18353461	18353287	ID=Msa1143150-mRNA-1:exon:4100;Parent=Msa1143150-mRNA-1
chr7_4	exon	18353853	18353971	18353853	ID=Msa1143150-mRNA-1:exon:4101;Parent=Msa1143150-mRNA-1
chr7_4	CDS	18353287	18353461	18353287	ID=Msa1143150-mRNA-1:cds;Parent=Msa1143150-mRNA-1
chr7_4	CDS	18353853	18353971	18353853	ID=Msa1143150-mRNA-1:cds;Parent=Msa1143150-mRNA-1

Gene Sequence	>Msa1143150 ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA
Protein sequence	>Msa1143150 MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*