AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268180


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268180 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268180 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268180 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268180 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa0268180 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 2_2:-70118368 Msa0268180:CDS
ACTGGCATAAAATCTACAAT+TGG 0.453871 2_2:+70118265 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 2_2:-70117718 Msa0268180:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 2_2:-70117730 Msa0268180:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 2_2:+70117712 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 2_2:+70117725 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG - chr2_2:70117994-70118013 Msa0268180:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG + chr2_2:70118000-70118019 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG + chr2_2:70118151-70118170 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG + chr2_2:70117992-70118011 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG - chr2_2:70118246-70118265 Msa0268180:CDS 15.0%
!! GAAAAAACTACTTGAATAAA+GGG + chr2_2:70117751-70117770 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG + chr2_2:70117752-70117771 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG - chr2_2:70117752-70117771 Msa0268180:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG - chr2_2:70118038-70118057 Msa0268180:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG - chr2_2:70118089-70118108 Msa0268180:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG - chr2_2:70118180-70118199 Msa0268180:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG - chr2_2:70117844-70117863 Msa0268180:intron 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG - chr2_2:70118321-70118340 Msa0268180:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG + chr2_2:70117793-70117812 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG + chr2_2:70117810-70117829 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG + chr2_2:70117828-70117847 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG + chr2_2:70117837-70117856 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG + chr2_2:70117838-70117857 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG + chr2_2:70118070-70118089 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG + chr2_2:70118073-70118092 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG - chr2_2:70118071-70118090 Msa0268180:intron 35.0%
CATATCCACAATCAAGATCT+AGG + chr2_2:70118079-70118098 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG - chr2_2:70118223-70118242 Msa0268180:CDS 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG + chr2_2:70117786-70117805 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG - chr2_2:70118342-70118361 Msa0268180:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG + chr2_2:70118350-70118369 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG + chr2_2:70118067-70118086 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG - chr2_2:70118354-70118373 Msa0268180:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG + chr2_2:70117915-70117934 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG + chr2_2:70118363-70118382 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70117705 70118389 70117705 ID=Msa0268180;Name=Msa0268180
chr2_2 mRNA 70117705 70118389 70117705 ID=Msa0268180-mRNA-1;Parent=Msa0268180;Name=Msa0268180-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr2_2 exon 70118215 70118389 70118215 ID=Msa0268180-mRNA-1:exon:22520;Parent=Msa0268180-mRNA-1
chr2_2 exon 70117705 70117823 70117705 ID=Msa0268180-mRNA-1:exon:22519;Parent=Msa0268180-mRNA-1
chr2_2 CDS 70118215 70118389 70118215 ID=Msa0268180-mRNA-1:cds;Parent=Msa0268180-mRNA-1
chr2_2 CDS 70117705 70117823 70117705 ID=Msa0268180-mRNA-1:cds;Parent=Msa0268180-mRNA-1
Gene Sequence

>Msa0268180

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa0268180

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*