AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1086370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086370 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086370 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086370 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086370 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa1086370 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 7 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 7_2:+87354730 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 7_2:-87354833 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 7_2:+87355379 Msa1086370:CDS
ATTTCTTATTCTTCACGTCC+AGG 0.484075 7_2:-87355397 None:intergenic
GGATTCCTATCTAATGATCC+AGG 0.536202 7_2:+87355367 Msa1086370:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 7_2:-87355385 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 7_2:-87355372 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + chr7_2:87355020-87355039 Msa1086370:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - chr7_2:87355026-87355045 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - chr7_2:87355177-87355196 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - chr7_2:87355018-87355037 None:intergenic 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - chr7_2:87354777-87354796 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - chr7_2:87354778-87354797 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + chr7_2:87354778-87354797 Msa1086370:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + chr7_2:87355064-87355083 Msa1086370:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + chr7_2:87355115-87355134 Msa1086370:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + chr7_2:87355206-87355225 Msa1086370:intron 20.0%
!!! GCGTTTTTTTTTAATTATCT+TGG + chr7_2:87355271-87355290 Msa1086370:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + chr7_2:87354870-87354889 Msa1086370:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + chr7_2:87355346-87355365 Msa1086370:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - chr7_2:87354819-87354838 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - chr7_2:87354836-87354855 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - chr7_2:87354854-87354873 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - chr7_2:87354863-87354882 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - chr7_2:87354864-87354883 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - chr7_2:87355096-87355115 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - chr7_2:87355099-87355118 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + chr7_2:87355097-87355116 Msa1086370:intron 35.0%
CATATCCACAATCAAGATCT+AGG - chr7_2:87355105-87355124 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + chr7_2:87355249-87355268 Msa1086370:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - chr7_2:87354812-87354831 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + chr7_2:87355367-87355386 Msa1086370:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - chr7_2:87355375-87355394 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - chr7_2:87355093-87355112 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + chr7_2:87355379-87355398 Msa1086370:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - chr7_2:87354941-87354960 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - chr7_2:87355388-87355407 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87354731 87355414 87354731 ID=Msa1086370;Name=Msa1086370
chr7_2 mRNA 87354731 87355414 87354731 ID=Msa1086370-mRNA-1;Parent=Msa1086370;Name=Msa1086370-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr7_2 exon 87354731 87354905 87354731 ID=Msa1086370-mRNA-1:exon:23779;Parent=Msa1086370-mRNA-1
chr7_2 exon 87355296 87355414 87355296 ID=Msa1086370-mRNA-1:exon:23780;Parent=Msa1086370-mRNA-1
chr7_2 CDS 87354731 87354905 87354731 ID=Msa1086370-mRNA-1:cds;Parent=Msa1086370-mRNA-1
chr7_2 CDS 87355296 87355414 87355296 ID=Msa1086370-mRNA-1:cds;Parent=Msa1086370-mRNA-1
Gene Sequence

>Msa1086370

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa1086370

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*