AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268650 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268650 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268650 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268650 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa0268650 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 2_2:-70349601 Msa0268650:CDS
ACTGGCATAAAATCTACAAT+TGG 0.453871 2_2:+70349498 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 2_2:-70348954 Msa0268650:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 2_2:-70348966 Msa0268650:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 2_2:+70348948 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 2_2:+70348961 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCAAAAAAAAAAAACTT+AGG + chr2_2:70349384-70349403 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG + chr2_2:70349228-70349247 None:intergenic 15.0%
!!! GATTCCAAATATTAAAATTT+TGG - chr2_2:70349229-70349248 Msa0268650:intron 15.0%
!!! CTATCCAAAATTTTAATATT+TGG + chr2_2:70349236-70349255 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG - chr2_2:70349479-70349498 Msa0268650:CDS 15.0%
!! GAAAAAACTACTTGAATAAA+GGG + chr2_2:70348987-70349006 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG + chr2_2:70348988-70349007 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG - chr2_2:70348988-70349007 Msa0268650:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG - chr2_2:70349272-70349291 Msa0268650:intron 20.0%
!! TTGTGGATATGAAAAAAATT+TGG - chr2_2:70349322-70349341 Msa0268650:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG - chr2_2:70349413-70349432 Msa0268650:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG - chr2_2:70349080-70349099 Msa0268650:intron 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG - chr2_2:70349554-70349573 Msa0268650:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG + chr2_2:70349029-70349048 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG + chr2_2:70349046-70349065 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG + chr2_2:70349064-70349083 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG + chr2_2:70349073-70349092 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG + chr2_2:70349074-70349093 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG + chr2_2:70349304-70349323 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG + chr2_2:70349307-70349326 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG - chr2_2:70349305-70349324 Msa0268650:intron 35.0%
CATATCCACAATCAAGATCT+AGG + chr2_2:70349313-70349332 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG - chr2_2:70349456-70349475 Msa0268650:CDS 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG + chr2_2:70349022-70349041 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG - chr2_2:70349575-70349594 Msa0268650:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG + chr2_2:70349583-70349602 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG + chr2_2:70349301-70349320 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG - chr2_2:70349587-70349606 Msa0268650:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG + chr2_2:70349151-70349170 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG + chr2_2:70349596-70349615 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70348941 70349622 70348941 ID=Msa0268650;Name=Msa0268650
chr2_2 mRNA 70348941 70349622 70348941 ID=Msa0268650-mRNA-1;Parent=Msa0268650;Name=Msa0268650-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr2_2 exon 70349448 70349622 70349448 ID=Msa0268650-mRNA-1:exon:22609;Parent=Msa0268650-mRNA-1
chr2_2 exon 70348941 70349059 70348941 ID=Msa0268650-mRNA-1:exon:22608;Parent=Msa0268650-mRNA-1
chr2_2 CDS 70349448 70349622 70349448 ID=Msa0268650-mRNA-1:cds;Parent=Msa0268650-mRNA-1
chr2_2 CDS 70348941 70349059 70348941 ID=Msa0268650-mRNA-1:cds;Parent=Msa0268650-mRNA-1
Gene Sequence

>Msa0268650

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa0268650

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*