AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1086440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086440 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086440 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086440 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086440 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa1086440 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 7_2:+87402168 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 7_2:-87402271 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 7_2:+87402816 Msa1086440:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 7_2:+87402804 Msa1086440:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 7_2:-87402822 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 7_2:-87402809 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + chr7_2:87402458-87402477 Msa1086440:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - chr7_2:87402464-87402483 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - chr7_2:87402613-87402632 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - chr7_2:87402456-87402475 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + chr7_2:87402708-87402727 Msa1086440:intron 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - chr7_2:87402215-87402234 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - chr7_2:87402216-87402235 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + chr7_2:87402216-87402235 Msa1086440:CDS 20.0%
!! ATGAAAATCTATTATTCGAT+TGG + chr7_2:87402501-87402520 Msa1086440:intron 20.0%
!!! TTGTGGATATGAAAAAATTT+TGG + chr7_2:87402551-87402570 Msa1086440:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + chr7_2:87402642-87402661 Msa1086440:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + chr7_2:87402308-87402327 Msa1086440:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + chr7_2:87402783-87402802 Msa1086440:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - chr7_2:87402257-87402276 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - chr7_2:87402274-87402293 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - chr7_2:87402292-87402311 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - chr7_2:87402301-87402320 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - chr7_2:87402302-87402321 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - chr7_2:87402533-87402552 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - chr7_2:87402536-87402555 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + chr7_2:87402534-87402553 Msa1086440:intron 35.0%
CATATCCACAATCAAGATCT+AGG - chr7_2:87402542-87402561 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + chr7_2:87402685-87402704 Msa1086440:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - chr7_2:87402250-87402269 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + chr7_2:87402804-87402823 Msa1086440:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - chr7_2:87402812-87402831 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - chr7_2:87402530-87402549 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + chr7_2:87402816-87402835 Msa1086440:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - chr7_2:87402379-87402398 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - chr7_2:87402825-87402844 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87402169 87402851 87402169 ID=Msa1086440;Name=Msa1086440
chr7_2 mRNA 87402169 87402851 87402169 ID=Msa1086440-mRNA-1;Parent=Msa1086440;Name=Msa1086440-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|0|0|2|0|97
chr7_2 exon 87402169 87402343 87402169 ID=Msa1086440-mRNA-1:exon:23843;Parent=Msa1086440-mRNA-1
chr7_2 exon 87402733 87402851 87402733 ID=Msa1086440-mRNA-1:exon:23844;Parent=Msa1086440-mRNA-1
chr7_2 CDS 87402169 87402343 87402169 ID=Msa1086440-mRNA-1:cds;Parent=Msa1086440-mRNA-1
chr7_2 CDS 87402733 87402851 87402733 ID=Msa1086440-mRNA-1:cds;Parent=Msa1086440-mRNA-1
Gene Sequence

>Msa1086440

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa1086440

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*