AlfalfaGEDB | Msa0582530

M. sativa cultivar zhongmu-4 / Msa0582530

KEGG: K02710|K02712
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF02533.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0582530	RDY14692.1	90.722	97	9	0	1	97	4	100	7.75e-56	178

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0582530	sp\|Q49L15\|PSBK_EUCGG	91.667	60	5	0	1	60	1	60	1.80e-32	110

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017120	Msa0582530	0.802799	4.726826e-49	-8.615850e-47
Msa0035090	Msa0582530	0.808723	2.692571e-50	-8.615850e-47
Msa0040190	Msa0582530	0.808605	2.852612e-50	-8.615850e-47
Msa0065390	Msa0582530	0.804332	2.272966e-49	-8.615850e-47
Msa0092750	Msa0582530	0.800262	1.565352e-48	-8.615850e-47
Msa0094420	Msa0582530	0.800095	1.692320e-48	-8.615850e-47
Msa0095140	Msa0582530	0.845660	3.536404e-59	-8.615850e-47
Msa0095180	Msa0582530	0.849299	3.535723e-60	-8.615850e-47
Msa0095200	Msa0582530	0.871173	8.363540e-67	-8.615850e-47
Msa0095240	Msa0582530	0.817253	3.628553e-52	-8.615850e-47
Msa0095270	Msa0582530	0.842205	2.980868e-58	-8.615850e-47
Msa0095300	Msa0582530	0.836138	1.115411e-56	-8.615850e-47
Msa0110710	Msa0582530	0.808743	2.666234e-50	-8.615850e-47
Msa0134550	Msa0582530	0.805293	1.431397e-49	-8.615850e-47
Msa0139740	Msa0582530	0.801765	7.715974e-49	-8.615850e-47
Msa0141670	Msa0582530	0.818525	1.872861e-52	-8.615850e-47
Msa0175900	Msa0582530	0.809719	1.646811e-50	-8.615850e-47
Msa0221860	Msa0582530	0.819515	1.115486e-52	-8.615850e-47
Msa0222070	Msa0582530	0.821621	3.663325e-53	-8.615850e-47
Msa0222120	Msa0582530	0.818025	2.430368e-52	-8.615850e-47
Msa0224960	Msa0582530	0.809071	2.268222e-50	-8.615850e-47
Msa0225010	Msa0582530	0.809532	1.806238e-50	-8.615850e-47
Msa0227290	Msa0582530	0.816831	4.514000e-52	-8.615850e-47
Msa0417630	Msa0582530	0.803612	3.208144e-49	-8.615850e-47
Msa0427050	Msa0582530	0.806560	7.752180e-50	-8.615850e-47
Msa0473450	Msa0582530	0.800276	1.555133e-48	-8.615850e-47
Msa0263620	Msa0582530	0.819387	1.193124e-52	-8.615850e-47
Msa0268180	Msa0582530	0.919885	2.570836e-87	-8.615850e-47
Msa0268220	Msa0582530	0.815162	1.064528e-51	-8.615850e-47
Msa0268340	Msa0582530	0.824816	6.576235e-54	-8.615850e-47
Msa0268380	Msa0582530	0.876108	1.814625e-68	-8.615850e-47
Msa0268420	Msa0582530	0.905606	3.593475e-80	-8.615850e-47
Msa0268460	Msa0582530	0.826259	2.992856e-54	-8.615850e-47
Msa0268500	Msa0582530	0.820447	6.827205e-53	-8.615850e-47
Msa0268540	Msa0582530	0.884675	1.565765e-71	-8.615850e-47
Msa0268650	Msa0582530	0.886309	3.824968e-72	-8.615850e-47
Msa0268680	Msa0582530	0.800148	1.651241e-48	-8.615850e-47
Msa0268690	Msa0582530	0.804334	2.270445e-49	-8.615850e-47
Msa0268730	Msa0582530	0.858946	5.810888e-63	-8.615850e-47
Msa0268760	Msa0582530	0.805482	1.306563e-49	-8.615850e-47
Msa0268850	Msa0582530	0.898429	5.352402e-77	-8.615850e-47
Msa0268900	Msa0582530	0.823688	1.210388e-53	-8.615850e-47
Msa0268930	Msa0582530	0.874397	6.973630e-68	-8.615850e-47
Msa0269060	Msa0582530	0.906710	1.109107e-80	-8.615850e-47
Msa0269100	Msa0582530	0.823966	1.042195e-53	-8.615850e-47
Msa0304870	Msa0582530	0.800225	1.592612e-48	-8.615850e-47
Msa0306130	Msa0582530	0.817732	2.831062e-52	-8.615850e-47
Msa0341260	Msa0582530	0.800247	1.576576e-48	-8.615850e-47
Msa0344200	Msa0582530	0.860766	1.642770e-63	-8.615850e-47
Msa0344250	Msa0582530	0.804161	2.467012e-49	-8.615850e-47
Msa0344290	Msa0582530	0.805557	1.260195e-49	-8.615850e-47
Msa0547050	Msa0582530	0.807530	4.832084e-50	-8.615850e-47
Msa0558890	Msa0582530	0.804526	2.071250e-49	-8.615850e-47
Msa0582530	Msa0582570	0.807538	4.812920e-50	-8.615850e-47
Msa0582530	Msa0582580	0.821960	3.057677e-53	-8.615850e-47
Msa0582530	Msa0582620	0.860392	2.132766e-63	-8.615850e-47
Msa0582530	Msa0582710	0.934766	2.426953e-96	-8.615850e-47
Msa0582530	Msa0582740	0.869003	4.285740e-66	-8.615850e-47
Msa0582530	Msa0582830	0.915377	6.331432e-85	-8.615850e-47
Msa0582530	Msa0582900	0.853164	2.863876e-61	-8.615850e-47
Msa0582530	Msa0591370	0.803944	2.737474e-49	-8.615850e-47
Msa0582530	Msa0595780	0.801765	7.715028e-49	-8.615850e-47
Msa0582530	Msa0622390	0.800755	1.241889e-48	-8.615850e-47
Msa0582530	Msa0647110	0.803509	3.369589e-49	-8.615850e-47
Msa0582530	Msa0653710	0.803292	3.736901e-49	-8.615850e-47
Msa0582530	Msa0702070	0.813893	2.032790e-51	-8.615850e-47
Msa0582530	Msa0702120	0.804795	1.819757e-49	-8.615850e-47
Msa0582530	Msa0727020	0.810653	1.035748e-50	-8.615850e-47
Msa0582530	Msa0739570	0.803750	3.003125e-49	-8.615850e-47
Msa0582530	Msa0766880	0.820131	8.063481e-53	-8.615850e-47
Msa0582530	Msa0772210	0.824776	6.720366e-54	-8.615850e-47
Msa0582530	Msa0782330	0.809740	1.629687e-50	-8.615850e-47
Msa0582530	Msa0804930	0.815871	7.403198e-52	-8.615850e-47
Msa0582530	Msa0860270	0.810717	1.003483e-50	-8.615850e-47
Msa0582530	Msa0900320	0.807753	4.332822e-50	-8.615850e-47
Msa0582530	Msa0924860	0.829238	5.757787e-55	-8.615850e-47
Msa0582530	Msa0924890	0.806012	1.011583e-49	-8.615850e-47
Msa0582530	Msa0931090	0.898071	7.592619e-77	-8.615850e-47
Msa0582530	Msa0931150	0.883057	6.190778e-71	-8.615850e-47
Msa0582530	Msa0931200	0.828316	9.620602e-55	-8.615850e-47
Msa0582530	Msa0931280	0.872374	3.340761e-67	-8.615850e-47
Msa0582530	Msa0983710	0.846462	2.139000e-59	-8.615850e-47
Msa0582530	Msa0992570	0.817613	3.011501e-52	-8.615850e-47
Msa0582530	Msa0992590	0.826531	2.578354e-54	-8.615850e-47
Msa0582530	Msa1002040	0.833594	4.876415e-56	-8.615850e-47
Msa0582530	Msa1030280	0.840442	8.673752e-58	-8.615850e-47
Msa0582530	Msa1070900	0.811516	6.733056e-51	-8.615850e-47
Msa0582530	Msa1086020	0.832260	1.046499e-55	-8.615850e-47
Msa0582530	Msa1086060	0.871474	6.646765e-67	-8.615850e-47
Msa0582530	Msa1086160	0.856384	3.335619e-62	-8.615850e-47
Msa0582530	Msa1086170	0.898434	5.324279e-77	-8.615850e-47
Msa0582530	Msa1086260	0.810004	1.429892e-50	-8.615850e-47
Msa0582530	Msa1086290	0.882475	1.010276e-70	-8.615850e-47
Msa0582530	Msa1086330	0.808282	3.343107e-50	-8.615850e-47
Msa0582530	Msa1086340	0.827933	1.189810e-54	-8.615850e-47
Msa0582530	Msa1086370	0.892830	1.105372e-74	-8.615850e-47
Msa0582530	Msa1086440	0.831119	1.999726e-55	-8.615850e-47
Msa0582530	Msa1086480	0.880683	4.484621e-70	-8.615850e-47
Msa0582530	Msa1086520	0.805050	1.609553e-49	-8.615850e-47
Msa0582530	Msa1086630	0.820279	7.459549e-53	-8.615850e-47
Msa0582530	Msa1086690	0.825020	5.885915e-54	-8.615850e-47
Msa0582530	Msa1086830	0.815336	9.742733e-52	-8.615850e-47
Msa0582530	Msa1086870	0.878275	3.203037e-69	-8.615850e-47
Msa0582530	Msa1090230	0.802566	5.279935e-49	-8.615850e-47
Msa0582530	Msa1091690	0.832793	7.716876e-56	-8.615850e-47
Msa0582530	Msa1143140	0.874244	7.856614e-68	-8.615850e-47
Msa0582530	Msa1143150	0.804424	2.174291e-49	-8.615850e-47
Msa0582530	Msa1157160	0.823548	1.305655e-53	-8.615850e-47
Msa0582530	Msa1195860	0.812844	3.455818e-51	-8.615850e-47
Msa0582530	Msa1284030	0.811349	7.321094e-51	-8.615850e-47
Msa0582530	Msa1298740	0.803076	4.141810e-49	-8.615850e-47
Msa0582530	Msa1305080	0.823066	1.691771e-53	-8.615850e-47
Msa0582530	Msa1309910	0.810748	9.877312e-51	-8.615850e-47
Msa0582530	Msa1323060	0.811766	5.941003e-51	-8.615850e-47
Msa0582530	Msa1327820	0.822588	2.185514e-53	-8.615850e-47
Msa0582530	Msa1342030	0.829048	6.402193e-55	-8.615850e-47
Msa0582530	Msa1352820	0.811813	5.803573e-51	-8.615850e-47
Msa0582530	Msa1354850	0.831089	2.033900e-55	-8.615850e-47
Msa0582530	Msa1354890	0.900646	5.946860e-78	-8.615850e-47
Msa0582530	Msa1355020	0.832765	7.840975e-56	-8.615850e-47
Msa0582530	Msa1355060	0.872301	3.532496e-67	-8.615850e-47
Msa0582530	Msa1367940	0.816389	5.670757e-52	-8.615850e-47
Msa0582530	Msa1380540	0.832242	1.056793e-55	-8.615850e-47
Msa0582530	Msa1380580	0.819595	1.069413e-52	-8.615850e-47
Msa0582530	Msa1385940	0.801779	7.665391e-49	-8.615850e-47
Msa0582530	Msa1387090	0.801701	7.951578e-49	-8.615850e-47
Msa0582530	Msa1394050	0.846238	2.462964e-59	-8.615850e-47
Msa0582530	Msa1394090	0.821696	3.520563e-53	-8.615850e-47
Msa0582530	Msa1394100	0.819781	9.697379e-53	-8.615850e-47
Msa0582530	Msa1394140	0.841409	4.836897e-58	-8.615850e-47
Msa0582530	Msa1394170	0.803209	3.888760e-49	-8.615850e-47
Msa0582530	Msa1394240	0.898452	5.231961e-77	-8.615850e-47
Msa0582530	Msa1394280	0.819008	1.455221e-52	-8.615850e-47
Msa0582530	Msa1406230	0.801045	1.083446e-48	-8.615850e-47
Msa0582530	Msa1422750	0.807084	6.007944e-50	-8.615850e-47
Msa0582530	Msa1435470	0.801936	7.115046e-49	-8.615850e-47
Msa0582530	Msa1442300	0.802159	6.404180e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0582530	MtrunA17_Chr4g0015681	96.667	60	2	1	60	1	60	7.64e-35	113
Msa0582530	MtrunA17_CPg0492901	96.667	60	2	1	60	1	60	7.64e-35	113
Msa0582530	MtrunA17_Chr4g0016771	96.667	60	2	1	60	1	60	7.64e-35	113

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0582530	ATCG00070.1	76.667	60	14	0	1	60	1	60	3.76e-26	92.0
Msa0582530	ATCG00080.1	97.222	36	1	0	62	97	1	36	2.92e-19	73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGCTTAATATCTTTAGCT+TGG	0.440922	4_2:-1062183	Msa0582530:CDS
ACTGGCATAAAATCTACAAT+TGG	0.453871	4_2:+1062080	None:intergenic
AATGATCCAGGACGTAATCC+TGG	0.474800	4_2:-1061533	Msa0582530:CDS
GGATTCCTATCTAATGATCC+AGG	0.536202	4_2:-1061545	Msa0582530:CDS
TCACGTCCAGGATTACGTCC+TGG	0.548081	4_2:+1061527	None:intergenic
TACGTCCTGGATCATTAGAT+AGG	0.581605	4_2:+1061540	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTCCAAATATTAAAATTTT+TGG	-	chr4_2:1061809-1061828	Msa0582530:intron	10.0%
!!!	TATCCAAAAATTTTAATATT+TGG	+	chr4_2:1061815-1061834	None:intergenic	10.0%
!!	ATTTCAAAAAAAAAAAACTT+AGG	+	chr4_2:1061966-1061985	None:intergenic	10.0%
!!!	AATTTTAATATTTGGAATCA+AGG	+	chr4_2:1061807-1061826	None:intergenic	15.0%
!!!	CGTTTTTTTTTTAATTATCT+TGG	-	chr4_2:1062061-1062080	Msa0582530:CDS	15.0%
!!	GAAAAAACTACTTGAATAAA+GGG	+	chr4_2:1061566-1061585	None:intergenic	20.0%
!!	AGAAAAAACTACTTGAATAA+AGG	+	chr4_2:1061567-1061586	None:intergenic	20.0%
!!!	TTATTCAAGTAGTTTTTTCT+TGG	-	chr4_2:1061567-1061586	Msa0582530:CDS	20.0%
!!	TGAAAATTCTATTATTCGAT+TGG	-	chr4_2:1061853-1061872	Msa0582530:intron	20.0%
!!!	TGTGGATATGAAAAAAATTT+TGG	-	chr4_2:1061904-1061923	Msa0582530:intron	20.0%
!!	TCTTATAAACTTAGTATCAA+AGG	-	chr4_2:1061995-1062014	Msa0582530:intron	20.0%
!!!	TTTTTCTCTTAGCTTTTGTT+TGG	-	chr4_2:1061659-1061678	Msa0582530:intron	25.0%
!!!	TTTGTTTCTCTTTTCATCTT+CGG	-	chr4_2:1062136-1062155	Msa0582530:CDS	25.0%
!	AATTGGATTCAAAAAAGCGT+AGG	+	chr4_2:1061608-1061627	None:intergenic	30.0%
	ACTGGCATAAAATCTACAAT+TGG	+	chr4_2:1061625-1061644	None:intergenic	30.0%
!!	AAAAAGAGTAAGGGTATTAC+TGG	+	chr4_2:1061643-1061662	None:intergenic	30.0%
	GCTAAGAGAAAAAAGAGTAA+GGG	+	chr4_2:1061652-1061671	None:intergenic	30.0%
	AGCTAAGAGAAAAAAGAGTA+AGG	+	chr4_2:1061653-1061672	None:intergenic	30.0%
!!	AATCAAGATCTAGGTATTGG+TGG	+	chr4_2:1061885-1061904	None:intergenic	35.0%
	CACAATCAAGATCTAGGTAT+TGG	+	chr4_2:1061888-1061907	None:intergenic	35.0%
	CAATACCTAGATCTTGATTG+TGG	-	chr4_2:1061886-1061905	Msa0582530:intron	35.0%
	CATATCCACAATCAAGATCT+AGG	+	chr4_2:1061894-1061913	None:intergenic	35.0%
	GAGAATCTATTCTCTTTCTC+TGG	-	chr4_2:1062038-1062057	Msa0582530:CDS	35.0%
!	TTCAAAAAAGCGTAGGCTTC+AGG	+	chr4_2:1061601-1061620	None:intergenic	40.0%
	GGATTCCTATCTAATGATCC+AGG	-	chr4_2:1062157-1062176	Msa0582530:CDS	40.0%
	TACGTCCTGGATCATTAGAT+AGG	+	chr4_2:1062165-1062184	None:intergenic	40.0%
!!	CAAGATCTAGGTATTGGTGG+TGG	+	chr4_2:1061882-1061901	None:intergenic	45.0%
	AATGATCCAGGACGTAATCC+TGG	-	chr4_2:1062169-1062188	Msa0582530:CDS	45.0%
!!!	AATAAATTCTTAATTTTTTT+CGG	+	chr4_2:1061730-1061749	None:intergenic	5.0%
	TCACGTCCAGGATTACGTCC+TGG	+	chr4_2:1062178-1062197	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	1061520	1062204	1061520	ID=Msa0582530;Name=Msa0582530
chr4_2	mRNA	1061520	1062204	1061520	ID=Msa0582530-mRNA-1;Parent=Msa0582530;Name=Msa0582530-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0\|0\|0\|1\|0\|0\|2\|0\|97
chr4_2	exon	1062030	1062204	1062030	ID=Msa0582530-mRNA-1:exon:219;Parent=Msa0582530-mRNA-1
chr4_2	exon	1061520	1061638	1061520	ID=Msa0582530-mRNA-1:exon:218;Parent=Msa0582530-mRNA-1
chr4_2	CDS	1062030	1062204	1062030	ID=Msa0582530-mRNA-1:cds;Parent=Msa0582530-mRNA-1
chr4_2	CDS	1061520	1061638	1061520	ID=Msa0582530-mRNA-1:cds;Parent=Msa0582530-mRNA-1

Gene Sequence	>Msa0582530 ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA
Protein sequence	>Msa0582530 MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*