AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1354890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354890 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1354890 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1354890 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354890 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa1354890 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 tig0014543:+580514 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 tig0014543:-580617 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 tig0014543:+581164 Msa1354890:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 tig0014543:+581152 Msa1354890:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 tig0014543:-581170 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 tig0014543:-581157 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + tig0014543:580804-580823 Msa1354890:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - tig0014543:580810-580829 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - tig0014543:580961-580980 None:intergenic 10.0%
!!! ATTCCAAATATTAAAATTTT+TGG + tig0014543:580804-580823 Msa1354890:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - tig0014543:580810-580829 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - tig0014543:580961-580980 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - tig0014543:580802-580821 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + tig0014543:581056-581075 Msa1354890:intron 15.0%
!!! AATTTTAATATTTGGAATCA+AGG - tig0014543:580802-580821 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + tig0014543:581056-581075 Msa1354890:intron 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - tig0014543:580561-580580 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - tig0014543:580562-580581 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + tig0014543:580562-580581 Msa1354890:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + tig0014543:580848-580867 Msa1354890:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + tig0014543:580899-580918 Msa1354890:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + tig0014543:580990-581009 Msa1354890:intron 20.0%
!! GAAAAAACTACTTGAATAAA+GGG - tig0014543:580561-580580 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - tig0014543:580562-580581 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + tig0014543:580562-580581 Msa1354890:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + tig0014543:580848-580867 Msa1354890:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + tig0014543:580899-580918 Msa1354890:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + tig0014543:580990-581009 Msa1354890:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + tig0014543:580654-580673 Msa1354890:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + tig0014543:581131-581150 Msa1354890:CDS 25.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + tig0014543:580654-580673 Msa1354890:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + tig0014543:581131-581150 Msa1354890:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - tig0014543:580603-580622 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - tig0014543:580620-580639 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - tig0014543:580638-580657 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - tig0014543:580647-580666 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - tig0014543:580648-580667 None:intergenic 30.0%
! AATTGGATTCAAAAAAGCGT+AGG - tig0014543:580603-580622 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - tig0014543:580620-580639 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - tig0014543:580638-580657 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - tig0014543:580647-580666 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - tig0014543:580648-580667 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - tig0014543:580880-580899 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - tig0014543:580883-580902 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + tig0014543:580881-580900 Msa1354890:intron 35.0%
CATATCCACAATCAAGATCT+AGG - tig0014543:580889-580908 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + tig0014543:581033-581052 Msa1354890:intron 35.0%
!! AATCAAGATCTAGGTATTGG+TGG - tig0014543:580880-580899 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - tig0014543:580883-580902 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + tig0014543:580881-580900 Msa1354890:intron 35.0%
CATATCCACAATCAAGATCT+AGG - tig0014543:580889-580908 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + tig0014543:581033-581052 Msa1354890:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - tig0014543:580596-580615 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + tig0014543:581152-581171 Msa1354890:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - tig0014543:581160-581179 None:intergenic 40.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - tig0014543:580596-580615 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + tig0014543:581152-581171 Msa1354890:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - tig0014543:581160-581179 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - tig0014543:580877-580896 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + tig0014543:581164-581183 Msa1354890:CDS 45.0%
!! CAAGATCTAGGTATTGGTGG+TGG - tig0014543:580877-580896 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + tig0014543:581164-581183 Msa1354890:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - tig0014543:580725-580744 None:intergenic 5.0%
!!! AATAAATTCTTAATTTTTTT+CGG - tig0014543:580725-580744 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - tig0014543:581173-581192 None:intergenic 55.0%
TCACGTCCAGGATTACGTCC+TGG - tig0014543:581173-581192 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0014543 gene 580515 581199 580515 ID=Msa1354890;Name=Msa1354890
tig0014543 mRNA 580515 581199 580515 ID=Msa1354890-mRNA-1;Parent=Msa1354890;Name=Msa1354890-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
tig0014543 exon 580515 580689 580515 ID=Msa1354890-mRNA-1:exon:9264;Parent=Msa1354890-mRNA-1
tig0014543 exon 581081 581199 581081 ID=Msa1354890-mRNA-1:exon:9265;Parent=Msa1354890-mRNA-1
tig0014543 CDS 580515 580689 580515 ID=Msa1354890-mRNA-1:cds;Parent=Msa1354890-mRNA-1
tig0014543 CDS 581081 581199 581081 ID=Msa1354890-mRNA-1:cds;Parent=Msa1354890-mRNA-1
Gene Sequence

>Msa1354890

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa1354890

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*