AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0125550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0125550 MtrunA17_Chr1g0186341 95.667 300 11 2 1 300 1 298 0.0 575
Msa0125550 MtrunA17_Chr1g0183391 27.729 339 187 10 1 299 1 321 1.66e-14 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0125550 AT2G33400.1 29.545 308 139 10 1 289 1 249 1.82e-24 100
Msa0125550 AT5G44040.1 33.108 296 145 11 30 299 71 339 1.06e-18 86.3
Msa0125550 AT1G04030.2 27.554 323 177 11 1 286 1 303 7.75e-13 68.9
Msa0125550 AT1G04030.1 27.554 323 177 11 1 286 1 303 7.75e-13 68.9

Find 60 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAAAGAAAGAAAGAAAA+TGG 0.158174 1_3:+54014940 Msa0125550:exon
CCCTAGTTCTTATCAGGTTT+TGG 0.222472 1_3:+54015379 Msa0125550:CDS
GATGAAAGTTTGGAGAATTA+TGG 0.268950 1_3:+54015611 Msa0125550:CDS
GGCAAACCCTAGTTCTTATC+AGG 0.299458 1_3:+54015373 Msa0125550:CDS
ACAAACCTGATTTCAGAATA+TGG 0.325794 1_3:-54015176 None:intergenic
TTTCTTTCTTTCTCTTTCAA+TGG 0.327083 1_3:-54014928 None:intergenic
TTGTACCATATTCTGAAATC+AGG 0.328302 1_3:+54015171 Msa0125550:CDS
TGATGAGGAAGATGAAAATA+AGG 0.334449 1_3:+54015409 Msa0125550:CDS
ATGAAAGTTTGGAGAATTAT+GGG 0.338370 1_3:+54015612 Msa0125550:CDS
TTGGGATGATGATGAAAGTT+TGG 0.373619 1_3:+54015601 Msa0125550:CDS
TTCATTCATTAAAAGTTTGT+TGG 0.384333 1_3:+54016112 Msa0125550:three_prime_UTR
TCATTCATTAAAAGTTTGTT+GGG 0.396716 1_3:+54016113 Msa0125550:three_prime_UTR
GGAGGATGAAATAGTGTATG+AGG 0.408167 1_3:+54015529 Msa0125550:CDS
AAGAAAGAAAGAAAGAAAAT+GGG 0.409644 1_3:+54014941 Msa0125550:exon
TCAGTCGAAGCCTTTGTTGC+AGG 0.410456 1_3:+54015931 Msa0125550:CDS
GAAGTTCTCGCCTTGCGTCT+TGG 0.432670 1_3:+54015895 Msa0125550:CDS
AATTTGATGATGGGTATGAT+TGG 0.438767 1_3:+54015582 Msa0125550:CDS
AACAGTGAACAGAAAGAAGA+TGG 0.438966 1_3:+54015656 Msa0125550:CDS
TGCTAGTCTTTCAAACTGGT+TGG 0.449139 1_3:+54015967 Msa0125550:CDS
GATGATGATGAATTTGATGA+TGG 0.451253 1_3:+54015572 Msa0125550:CDS
AAGCATGCTGCCTGTAGTCT+TGG 0.451334 1_3:+54015862 Msa0125550:CDS
CAACAAAGGCTTCGACTGAA+TGG 0.464145 1_3:-54015927 None:intergenic
ACTGCAATTTCCTGCAACAA+AGG 0.476203 1_3:-54015941 None:intergenic
TGTTGCAGCTATGCAATTTG+AGG 0.478704 1_3:+54016251 Msa0125550:three_prime_UTR
TTGATGCTAGTCTTTCAAAC+TGG 0.480004 1_3:+54015963 Msa0125550:CDS
TAGAGGTAGAGATCAAGAAA+AGG 0.488908 1_3:+54015283 Msa0125550:intron
CATTGAGTAAGATTCTCTAC+CGG 0.490839 1_3:-54015767 None:intergenic
CGGTAGAGAATCTTACTCAA+TGG 0.500520 1_3:+54015768 Msa0125550:CDS
TTTGAATGTAGAGATGTATG+AGG 0.505130 1_3:+54015352 Msa0125550:CDS
TGTCTAAAACAGAAACTCGT+TGG 0.507304 1_3:+54014751 Msa0125550:five_prime_UTR
ACTCTCTACAAAGTCCTAGC+TGG 0.507971 1_3:+54015013 Msa0125550:CDS
GAGCTTGATTCCAAGACTAC+AGG 0.512568 1_3:-54015872 None:intergenic
ATAAGAAAGAATGTTTATGT+TGG 0.519157 1_3:-54014893 None:intergenic
GTCAAGTAAACAAACGAGAA+AGG 0.525056 1_3:+54015814 Msa0125550:CDS
TATAATATCTATGATTCGAC+GGG 0.526124 1_3:-54015484 None:intergenic
TCCAAAACCTGATAAGAACT+AGG 0.528369 1_3:-54015380 None:intergenic
CATGAATTCCGTACTGCACC+CGG 0.549273 1_3:+54015748 Msa0125550:CDS
TTATAATATCTATGATTCGA+CGG 0.553522 1_3:-54015485 None:intergenic
CCAAAACCTGATAAGAACTA+GGG 0.562445 1_3:-54015379 None:intergenic
ATTTGATGATGGGTATGATT+GGG 0.562602 1_3:+54015583 Msa0125550:CDS
AGAAACTCGTTGGAGCCAAA+CGG 0.563148 1_3:+54014761 Msa0125550:five_prime_UTR
GATCAAGAAAAGGAGAAGAG+TGG 0.564885 1_3:+54015293 Msa0125550:CDS
TTTCTGTTTCTTCAAGCACT+CGG 0.567496 1_3:-54016039 None:intergenic
TAGTTATGACAATGAGGAGG+AGG 0.567704 1_3:+54015511 Msa0125550:CDS
ATGATGATGAATTTGATGAT+GGG 0.572566 1_3:+54015573 Msa0125550:CDS
ATTCTCTACCGGGTGCAGTA+CGG 0.574059 1_3:-54015756 None:intergenic
ACAGTGAACAGAAAGAAGAT+GGG 0.578317 1_3:+54015657 Msa0125550:CDS
ATTGAGTAAGATTCTCTACC+GGG 0.594847 1_3:-54015766 None:intergenic
CGAATTTGAGTGGACGAGAG+AGG 0.606574 1_3:+54015717 Msa0125550:CDS
TCATTGACAACGAATTGTAG+TGG 0.620784 1_3:-54016011 None:intergenic
ACAATTCGTTGTCAATGATG+TGG 0.639822 1_3:+54016016 Msa0125550:exon
ATCGAATTGTGATGCTTCTG+TGG 0.643973 1_3:+54016081 Msa0125550:three_prime_UTR
TGAAAATAAGGAGACTACTG+TGG 0.644236 1_3:+54015421 Msa0125550:CDS
TAACTGTAGTTATGACAATG+AGG 0.650246 1_3:+54015505 Msa0125550:CDS
GCATTCGATTCCAAGACGCA+AGG 0.653152 1_3:-54015905 None:intergenic
GAGTTGAAATCGAATTTGAG+TGG 0.654782 1_3:+54015707 Msa0125550:CDS
GTAGTGGAGACATTATAACT+AGG 0.665616 1_3:-54015995 None:intergenic
TGAAAGTTTGGAGAATTATG+GGG 0.678615 1_3:+54015613 Msa0125550:CDS
ACTGTGGAATCAGAGAGAGA+AGG 0.685984 1_3:+54015437 Msa0125550:CDS
CTGTAGTTATGACAATGAGG+AGG 0.688122 1_3:+54015508 Msa0125550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATTTTATTTGTTAG+AGG + chr1_3:54015266-54015285 Msa0125550:intron 10.0%
!!! TTTTTTATTTTATTTGTTAG+AGG + chr1_3:54015266-54015285 Msa0125550:intron 10.0%
!! ATAAGAAAGAATGTTTATGT+TGG - chr1_3:54014896-54014915 None:intergenic 20.0%
!! AAAGAAAGAAAGAAAGAAAA+TGG + chr1_3:54014940-54014959 Msa0125550:exon 20.0%
!! AAGAAAGAAAGAAAGAAAAT+GGG + chr1_3:54014941-54014960 Msa0125550:exon 20.0%
!! TGAAGAAATATTATTGTATG+TGG + chr1_3:54015218-54015237 Msa0125550:intron 20.0%
!! TTATAATATCTATGATTCGA+CGG - chr1_3:54015488-54015507 None:intergenic 20.0%
!! TTCATTCATTAAAAGTTTGT+TGG + chr1_3:54016112-54016131 Msa0125550:three_prime_UTR 20.0%
!!! TCATTCATTAAAAGTTTGTT+GGG + chr1_3:54016113-54016132 Msa0125550:three_prime_UTR 20.0%
!! ATAAGAAAGAATGTTTATGT+TGG - chr1_3:54014896-54014915 None:intergenic 20.0%
!! AAAGAAAGAAAGAAAGAAAA+TGG + chr1_3:54014940-54014959 Msa0125550:exon 20.0%
!! AAGAAAGAAAGAAAGAAAAT+GGG + chr1_3:54014941-54014960 Msa0125550:exon 20.0%
!! TGAAGAAATATTATTGTATG+TGG + chr1_3:54015218-54015237 Msa0125550:intron 20.0%
!! TTATAATATCTATGATTCGA+CGG - chr1_3:54015488-54015507 None:intergenic 20.0%
!! TTCATTCATTAAAAGTTTGT+TGG + chr1_3:54016112-54016131 Msa0125550:three_prime_UTR 20.0%
!!! TCATTCATTAAAAGTTTGTT+GGG + chr1_3:54016113-54016132 Msa0125550:three_prime_UTR 20.0%
! TTTCTTTCTTTCTCTTTCAA+TGG - chr1_3:54014931-54014950 None:intergenic 25.0%
!!! GTTGTTTTGATATACTTACT+TGG - chr1_3:54015045-54015064 None:intergenic 25.0%
! ATCAAGAACTTAAGAATGAT+GGG - chr1_3:54015067-54015086 None:intergenic 25.0%
! AATCAAGAACTTAAGAATGA+TGG - chr1_3:54015068-54015087 None:intergenic 25.0%
!! CTTATCTTTTTGCAGAAATA+TGG + chr1_3:54015110-54015129 Msa0125550:intron 25.0%
! TATAATATCTATGATTCGAC+GGG - chr1_3:54015487-54015506 None:intergenic 25.0%
!! ATGATGATGAATTTGATGAT+GGG + chr1_3:54015573-54015592 Msa0125550:CDS 25.0%
! ATGAAAGTTTGGAGAATTAT+GGG + chr1_3:54015612-54015631 Msa0125550:CDS 25.0%
!!! TCCTGTAATTGTTTTGTTTT+TGG + chr1_3:54016169-54016188 Msa0125550:three_prime_UTR 25.0%
! ACCAAAAACAAAACAATTAC+AGG - chr1_3:54016173-54016192 None:intergenic 25.0%
!!! TTTTGTTTTTGGTTTGTGAA+TGG + chr1_3:54016180-54016199 Msa0125550:three_prime_UTR 25.0%
! TTTCTTTCTTTCTCTTTCAA+TGG - chr1_3:54014931-54014950 None:intergenic 25.0%
!!! GTTGTTTTGATATACTTACT+TGG - chr1_3:54015045-54015064 None:intergenic 25.0%
! ATCAAGAACTTAAGAATGAT+GGG - chr1_3:54015067-54015086 None:intergenic 25.0%
! AATCAAGAACTTAAGAATGA+TGG - chr1_3:54015068-54015087 None:intergenic 25.0%
!! CTTATCTTTTTGCAGAAATA+TGG + chr1_3:54015110-54015129 Msa0125550:intron 25.0%
! TATAATATCTATGATTCGAC+GGG - chr1_3:54015487-54015506 None:intergenic 25.0%
!! ATGATGATGAATTTGATGAT+GGG + chr1_3:54015573-54015592 Msa0125550:CDS 25.0%
! ATGAAAGTTTGGAGAATTAT+GGG + chr1_3:54015612-54015631 Msa0125550:CDS 25.0%
!!! TCCTGTAATTGTTTTGTTTT+TGG + chr1_3:54016169-54016188 Msa0125550:three_prime_UTR 25.0%
! ACCAAAAACAAAACAATTAC+AGG - chr1_3:54016173-54016192 None:intergenic 25.0%
!!! TTTTGTTTTTGGTTTGTGAA+TGG + chr1_3:54016180-54016199 Msa0125550:three_prime_UTR 25.0%
!! TTGTACCATATTCTGAAATC+AGG + chr1_3:54015171-54015190 Msa0125550:CDS 30.0%
ACAAACCTGATTTCAGAATA+TGG - chr1_3:54015179-54015198 None:intergenic 30.0%
TTTGAATGTAGAGATGTATG+AGG + chr1_3:54015352-54015371 Msa0125550:CDS 30.0%
TGATGAGGAAGATGAAAATA+AGG + chr1_3:54015409-54015428 Msa0125550:CDS 30.0%
TAACTGTAGTTATGACAATG+AGG + chr1_3:54015505-54015524 Msa0125550:CDS 30.0%
! GATGATGATGAATTTGATGA+TGG + chr1_3:54015572-54015591 Msa0125550:CDS 30.0%
!! AATTTGATGATGGGTATGAT+TGG + chr1_3:54015582-54015601 Msa0125550:CDS 30.0%
!! ATTTGATGATGGGTATGATT+GGG + chr1_3:54015583-54015602 Msa0125550:CDS 30.0%
GATGAAAGTTTGGAGAATTA+TGG + chr1_3:54015611-54015630 Msa0125550:CDS 30.0%
TGAAAGTTTGGAGAATTATG+GGG + chr1_3:54015613-54015632 Msa0125550:CDS 30.0%
!! TTGTACCATATTCTGAAATC+AGG + chr1_3:54015171-54015190 Msa0125550:CDS 30.0%
ACAAACCTGATTTCAGAATA+TGG - chr1_3:54015179-54015198 None:intergenic 30.0%
TTTGAATGTAGAGATGTATG+AGG + chr1_3:54015352-54015371 Msa0125550:CDS 30.0%
TGATGAGGAAGATGAAAATA+AGG + chr1_3:54015409-54015428 Msa0125550:CDS 30.0%
TAACTGTAGTTATGACAATG+AGG + chr1_3:54015505-54015524 Msa0125550:CDS 30.0%
! GATGATGATGAATTTGATGA+TGG + chr1_3:54015572-54015591 Msa0125550:CDS 30.0%
!! AATTTGATGATGGGTATGAT+TGG + chr1_3:54015582-54015601 Msa0125550:CDS 30.0%
!! ATTTGATGATGGGTATGATT+GGG + chr1_3:54015583-54015602 Msa0125550:CDS 30.0%
GATGAAAGTTTGGAGAATTA+TGG + chr1_3:54015611-54015630 Msa0125550:CDS 30.0%
TGAAAGTTTGGAGAATTATG+GGG + chr1_3:54015613-54015632 Msa0125550:CDS 30.0%
! TGTCTAAAACAGAAACTCGT+TGG + chr1_3:54014751-54014770 Msa0125550:five_prime_UTR 35.0%
!! GGTTGTTTTCTTCAGTGTTT+TGG + chr1_3:54014962-54014981 Msa0125550:CDS 35.0%
TAGAGGTAGAGATCAAGAAA+AGG + chr1_3:54015283-54015302 Msa0125550:intron 35.0%
CCAAAACCTGATAAGAACTA+GGG - chr1_3:54015382-54015401 None:intergenic 35.0%
TCCAAAACCTGATAAGAACT+AGG - chr1_3:54015383-54015402 None:intergenic 35.0%
TGAAAATAAGGAGACTACTG+TGG + chr1_3:54015421-54015440 Msa0125550:CDS 35.0%
! TTGGGATGATGATGAAAGTT+TGG + chr1_3:54015601-54015620 Msa0125550:CDS 35.0%
AACAGTGAACAGAAAGAAGA+TGG + chr1_3:54015656-54015675 Msa0125550:CDS 35.0%
ACAGTGAACAGAAAGAAGAT+GGG + chr1_3:54015657-54015676 Msa0125550:CDS 35.0%
GAGTTGAAATCGAATTTGAG+TGG + chr1_3:54015707-54015726 Msa0125550:CDS 35.0%
ATTGAGTAAGATTCTCTACC+GGG - chr1_3:54015769-54015788 None:intergenic 35.0%
CATTGAGTAAGATTCTCTAC+CGG - chr1_3:54015770-54015789 None:intergenic 35.0%
GTCAAGTAAACAAACGAGAA+AGG + chr1_3:54015814-54015833 Msa0125550:CDS 35.0%
!!! ATGCTTGTTTTTTGCTCTGA+TGG - chr1_3:54015848-54015867 None:intergenic 35.0%
!! TTGATGCTAGTCTTTCAAAC+TGG + chr1_3:54015963-54015982 Msa0125550:CDS 35.0%
! GTAGTGGAGACATTATAACT+AGG - chr1_3:54015998-54016017 None:intergenic 35.0%
TCATTGACAACGAATTGTAG+TGG - chr1_3:54016014-54016033 None:intergenic 35.0%
ACAATTCGTTGTCAATGATG+TGG + chr1_3:54016016-54016035 Msa0125550:exon 35.0%
! TTTCTGTTTCTTCAAGCACT+CGG - chr1_3:54016042-54016061 None:intergenic 35.0%
! TGTCTAAAACAGAAACTCGT+TGG + chr1_3:54014751-54014770 Msa0125550:five_prime_UTR 35.0%
!! GGTTGTTTTCTTCAGTGTTT+TGG + chr1_3:54014962-54014981 Msa0125550:CDS 35.0%
TAGAGGTAGAGATCAAGAAA+AGG + chr1_3:54015283-54015302 Msa0125550:intron 35.0%
CCAAAACCTGATAAGAACTA+GGG - chr1_3:54015382-54015401 None:intergenic 35.0%
TCCAAAACCTGATAAGAACT+AGG - chr1_3:54015383-54015402 None:intergenic 35.0%
TGAAAATAAGGAGACTACTG+TGG + chr1_3:54015421-54015440 Msa0125550:CDS 35.0%
! TTGGGATGATGATGAAAGTT+TGG + chr1_3:54015601-54015620 Msa0125550:CDS 35.0%
AACAGTGAACAGAAAGAAGA+TGG + chr1_3:54015656-54015675 Msa0125550:CDS 35.0%
ACAGTGAACAGAAAGAAGAT+GGG + chr1_3:54015657-54015676 Msa0125550:CDS 35.0%
GAGTTGAAATCGAATTTGAG+TGG + chr1_3:54015707-54015726 Msa0125550:CDS 35.0%
ATTGAGTAAGATTCTCTACC+GGG - chr1_3:54015769-54015788 None:intergenic 35.0%
CATTGAGTAAGATTCTCTAC+CGG - chr1_3:54015770-54015789 None:intergenic 35.0%
GTCAAGTAAACAAACGAGAA+AGG + chr1_3:54015814-54015833 Msa0125550:CDS 35.0%
!!! ATGCTTGTTTTTTGCTCTGA+TGG - chr1_3:54015848-54015867 None:intergenic 35.0%
!! TTGATGCTAGTCTTTCAAAC+TGG + chr1_3:54015963-54015982 Msa0125550:CDS 35.0%
! GTAGTGGAGACATTATAACT+AGG - chr1_3:54015998-54016017 None:intergenic 35.0%
TCATTGACAACGAATTGTAG+TGG - chr1_3:54016014-54016033 None:intergenic 35.0%
ACAATTCGTTGTCAATGATG+TGG + chr1_3:54016016-54016035 Msa0125550:exon 35.0%
! TTTCTGTTTCTTCAAGCACT+CGG - chr1_3:54016042-54016061 None:intergenic 35.0%
!!! GACAGAGTTTTAAAGCCGTT+TGG - chr1_3:54014779-54014798 None:intergenic 40.0%
!! TTACTTGGTTTTCTCCAGCT+AGG - chr1_3:54015030-54015049 None:intergenic 40.0%
! ATTGTATGTGGTTCTGTGAG+TGG + chr1_3:54015230-54015249 Msa0125550:intron 40.0%
GATCAAGAAAAGGAGAAGAG+TGG + chr1_3:54015293-54015312 Msa0125550:CDS 40.0%
!! CCCTAGTTCTTATCAGGTTT+TGG + chr1_3:54015379-54015398 Msa0125550:CDS 40.0%
!!! GGTTTTGGATGATGATGATG+AGG + chr1_3:54015394-54015413 Msa0125550:CDS 40.0%
CTGTAGTTATGACAATGAGG+AGG + chr1_3:54015508-54015527 Msa0125550:CDS 40.0%
TAGTTATGACAATGAGGAGG+AGG + chr1_3:54015511-54015530 Msa0125550:CDS 40.0%
GGAGGATGAAATAGTGTATG+AGG + chr1_3:54015529-54015548 Msa0125550:CDS 40.0%
CGGTAGAGAATCTTACTCAA+TGG + chr1_3:54015768-54015787 Msa0125550:CDS 40.0%
ACTGCAATTTCCTGCAACAA+AGG - chr1_3:54015944-54015963 None:intergenic 40.0%
!! TGCTAGTCTTTCAAACTGGT+TGG + chr1_3:54015967-54015986 Msa0125550:CDS 40.0%
ATCGAATTGTGATGCTTCTG+TGG + chr1_3:54016081-54016100 Msa0125550:three_prime_UTR 40.0%
CATGTTGCAGCTATGCAATT+TGG + chr1_3:54016249-54016268 Msa0125550:three_prime_UTR 40.0%
!!! GACAGAGTTTTAAAGCCGTT+TGG - chr1_3:54014779-54014798 None:intergenic 40.0%
!! TTACTTGGTTTTCTCCAGCT+AGG - chr1_3:54015030-54015049 None:intergenic 40.0%
! ATTGTATGTGGTTCTGTGAG+TGG + chr1_3:54015230-54015249 Msa0125550:intron 40.0%
GATCAAGAAAAGGAGAAGAG+TGG + chr1_3:54015293-54015312 Msa0125550:CDS 40.0%
!! CCCTAGTTCTTATCAGGTTT+TGG + chr1_3:54015379-54015398 Msa0125550:CDS 40.0%
!!! GGTTTTGGATGATGATGATG+AGG + chr1_3:54015394-54015413 Msa0125550:CDS 40.0%
CTGTAGTTATGACAATGAGG+AGG + chr1_3:54015508-54015527 Msa0125550:CDS 40.0%
TAGTTATGACAATGAGGAGG+AGG + chr1_3:54015511-54015530 Msa0125550:CDS 40.0%
GGAGGATGAAATAGTGTATG+AGG + chr1_3:54015529-54015548 Msa0125550:CDS 40.0%
CGGTAGAGAATCTTACTCAA+TGG + chr1_3:54015768-54015787 Msa0125550:CDS 40.0%
ACTGCAATTTCCTGCAACAA+AGG - chr1_3:54015944-54015963 None:intergenic 40.0%
!! TGCTAGTCTTTCAAACTGGT+TGG + chr1_3:54015967-54015986 Msa0125550:CDS 40.0%
ATCGAATTGTGATGCTTCTG+TGG + chr1_3:54016081-54016100 Msa0125550:three_prime_UTR 40.0%
AGAAACTCGTTGGAGCCAAA+CGG + chr1_3:54014761-54014780 Msa0125550:five_prime_UTR 45.0%
ACTCTCTACAAAGTCCTAGC+TGG + chr1_3:54015013-54015032 Msa0125550:CDS 45.0%
! GGCAAACCCTAGTTCTTATC+AGG + chr1_3:54015373-54015392 Msa0125550:CDS 45.0%
ACTGTGGAATCAGAGAGAGA+AGG + chr1_3:54015437-54015456 Msa0125550:CDS 45.0%
GAGCTTGATTCCAAGACTAC+AGG - chr1_3:54015875-54015894 None:intergenic 45.0%
CAACAAAGGCTTCGACTGAA+TGG - chr1_3:54015930-54015949 None:intergenic 45.0%
AGAAACTCGTTGGAGCCAAA+CGG + chr1_3:54014761-54014780 Msa0125550:five_prime_UTR 45.0%
ACTCTCTACAAAGTCCTAGC+TGG + chr1_3:54015013-54015032 Msa0125550:CDS 45.0%
! GGCAAACCCTAGTTCTTATC+AGG + chr1_3:54015373-54015392 Msa0125550:CDS 45.0%
ACTGTGGAATCAGAGAGAGA+AGG + chr1_3:54015437-54015456 Msa0125550:CDS 45.0%
GAGCTTGATTCCAAGACTAC+AGG - chr1_3:54015875-54015894 None:intergenic 45.0%
CAACAAAGGCTTCGACTGAA+TGG - chr1_3:54015930-54015949 None:intergenic 45.0%
CGAATTTGAGTGGACGAGAG+AGG + chr1_3:54015717-54015736 Msa0125550:CDS 50.0%
CATGAATTCCGTACTGCACC+CGG + chr1_3:54015748-54015767 Msa0125550:CDS 50.0%
ATTCTCTACCGGGTGCAGTA+CGG - chr1_3:54015759-54015778 None:intergenic 50.0%
AAGCATGCTGCCTGTAGTCT+TGG + chr1_3:54015862-54015881 Msa0125550:CDS 50.0%
GCATTCGATTCCAAGACGCA+AGG - chr1_3:54015908-54015927 None:intergenic 50.0%
TCAGTCGAAGCCTTTGTTGC+AGG + chr1_3:54015931-54015950 Msa0125550:CDS 50.0%
CGAATTTGAGTGGACGAGAG+AGG + chr1_3:54015717-54015736 Msa0125550:CDS 50.0%
CATGAATTCCGTACTGCACC+CGG + chr1_3:54015748-54015767 Msa0125550:CDS 50.0%
ATTCTCTACCGGGTGCAGTA+CGG - chr1_3:54015759-54015778 None:intergenic 50.0%
AAGCATGCTGCCTGTAGTCT+TGG + chr1_3:54015862-54015881 Msa0125550:CDS 50.0%
GCATTCGATTCCAAGACGCA+AGG - chr1_3:54015908-54015927 None:intergenic 50.0%
TCAGTCGAAGCCTTTGTTGC+AGG + chr1_3:54015931-54015950 Msa0125550:CDS 50.0%
GAAGTTCTCGCCTTGCGTCT+TGG + chr1_3:54015895-54015914 Msa0125550:CDS 55.0%
GAAGTTCTCGCCTTGCGTCT+TGG + chr1_3:54015895-54015914 Msa0125550:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 54014741 54016270 54014741 ID=Msa0125550;Name=Msa0125550
chr1_3 mRNA 54014741 54016270 54014741 ID=Msa0125550-mRNA-1;Parent=Msa0125550;Name=Msa0125550-mRNA-1;_AED=0.03;_eAED=0.03;_QI=218|1|1|1|1|1|3|237|300
chr1_3 exon 54014741 54015045 54014741 ID=Msa0125550-mRNA-1:exon:16608;Parent=Msa0125550-mRNA-1
chr1_3 exon 54015125 54015192 54015125 ID=Msa0125550-mRNA-1:exon:16609;Parent=Msa0125550-mRNA-1
chr1_3 exon 54015286 54016270 54015286 ID=Msa0125550-mRNA-1:exon:16610;Parent=Msa0125550-mRNA-1
chr1_3 five_prime_UTR 54014741 54014958 54014741 ID=Msa0125550-mRNA-1:five_prime_utr;Parent=Msa0125550-mRNA-1
chr1_3 CDS 54014959 54015045 54014959 ID=Msa0125550-mRNA-1:cds;Parent=Msa0125550-mRNA-1
chr1_3 CDS 54015125 54015192 54015125 ID=Msa0125550-mRNA-1:cds;Parent=Msa0125550-mRNA-1
chr1_3 CDS 54015286 54016033 54015286 ID=Msa0125550-mRNA-1:cds;Parent=Msa0125550-mRNA-1
chr1_3 three_prime_UTR 54016034 54016270 54016034 ID=Msa0125550-mRNA-1:three_prime_utr;Parent=Msa0125550-mRNA-1
Gene Sequence

>Msa0125550

ATGGGTTGTTTTCTTCAGTGTTTTGGTTTAACAAGCAAAAGAAAACGCAGAAAGACTCTCTACAAAGTCCTAGCTGGAGAAAACCAAAAATATGGAAACTATCAAGTTCTTGCTACTATTACAGAGAAATCTATTGTACCATATTCTGAAATCAGAGGTAGAGATCAAGAAAAGGAGAAGAGTGGTGTTAAGAGCAAGAAGAAGAAGAAAGTTAGCTTTAATTTGAATGTAGAGATGTATGAGGCAAACCCTAGTTCTTATCAGGTTTTGGATGATGATGATGAGGAAGATGAAAATAAGGAGACTACTGTGGAATCAGAGAGAGAAGGATCTGTAGTGTCGATGATTCGATACCCGTCGAATCATAGATATTATAACTGTAGTTATGACAATGAGGAGGAGGATGAAATAGTGTATGAGGAATCTGATATTGATGAGTTTGATGATGATGAATTTGATGATGGGTATGATTGGGATGATGATGAAAGTTTGGAGAATTATGGGGATGCTGAAGTTTGCGATGAAAACAGTGAACAGAAAGAAGATGGGATGAAGAATCAGAAGTCGAACGATGCTGAGTTGAAATCGAATTTGAGTGGACGAGAGAGGAGCATAAACATGAATTCCGTACTGCACCCGGTAGAGAATCTTACTCAATGGAAAGCGATCAAAGCAAAAGTTGCGTCAAGTAAACAAACGAGAAAGGAAAATGTTCCATCAGAGCAAAAAACAAGCATGCTGCCTGTAGTCTTGGAATCAAGCTCGAAGTTCTCGCCTTGCGTCTTGGAATCGAATGCCATTCAGTCGAAGCCTTTGTTGCAGGAAATTGCAGTTGATGCTAGTCTTTCAAACTGGTTGGTTTCTCCTAGTTATAATGTCTCCACTACAATTCGTTGTCAATGA
Protein sequence

>Msa0125550

MGCFLQCFGLTSKRKRRKTLYKVLAGENQKYGNYQVLATITEKSIVPYSEIRGRDQEKEKSGVKSKKKKKVSFNLNVEMYEANPSSYQVLDDDDEEDENKETTVESEREGSVVSMIRYPSNHRYYNCSYDNEEEDEIVYEESDIDEFDDDEFDDGYDWDDDESLENYGDAEVCDENSEQKEDGMKNQKSNDAELKSNLSGRERSINMNSVLHPVENLTQWKAIKAKVASSKQTRKENVPSEQKTSMLPVVLESSSKFSPCVLESNAIQSKPLLQEIAVDASLSNWLVSPSYNVSTTIRCQ*