AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0050380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0050380 MtrunA17_Chr4g0052371 86.992 123 4 4 1 122 1 112 1.50e-56 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCAGCAGACGATTATTT+TGG 0.182988 1_2:-4270057 Msa0050380:intron
TCCATTGTCCGGAACTCTTT+TGG 0.290169 1_2:-4270362 Msa0050380:CDS
TCAGGATTTAATTGAAATCA+AGG 0.374856 1_2:-4270166 Msa0050380:intron
TGCTGCAAAGCAGGCAGCCA+AGG 0.380640 1_2:-4269991 Msa0050380:CDS
TGAATTTGCCAAAAGAGTTC+CGG 0.381401 1_2:+4270354 None:intergenic
TCCCCTTCCGATTGGGTAGC+CGG 0.386098 1_2:+4270390 None:intergenic
CTTCCGAACTTTAGTTGCCT+TGG 0.396128 1_2:+4269974 None:intergenic
GAGTAAAGCTGCTGCAAAGC+AGG 0.435629 1_2:-4270000 Msa0050380:CDS
ATTAAATCCTGAGGAATGTT+GGG 0.440220 1_2:+4270177 None:intergenic
AATTAAATCCTGAGGAATGT+TGG 0.444643 1_2:+4270176 None:intergenic
CAAGCTTATGATTTCCTCAC+TGG 0.467518 1_2:-4270217 Msa0050380:CDS
GATTTACTCCCCTTCCGATT+GGG 0.479142 1_2:+4270383 None:intergenic
ACGTACGCCGGCTACCCAAT+CGG 0.500846 1_2:-4270397 Msa0050380:CDS
GGATTTACTCCCCTTCCGAT+TGG 0.503941 1_2:+4270382 None:intergenic
CAGCCAAGGCAACTAAAGTT+CGG 0.506043 1_2:-4269977 Msa0050380:CDS
GCAGAAGGGGAAGAAAGTGT+TGG 0.512031 1_2:-4269904 Msa0050380:CDS
GAAGGAGCCCAACATTCCTC+AGG 0.521672 1_2:-4270184 Msa0050380:CDS
ATTGGGTAGCCGGCGTACGT+TGG 0.539138 1_2:+4270400 None:intergenic
GCCAAAAGAGTTCCGGACAA+TGG 0.540316 1_2:+4270361 None:intergenic
AGTAAAATTCATAAGCAGAA+GGG 0.542068 1_2:-4269918 Msa0050380:CDS
AGGGGAGTAAATCCATTGTC+CGG 0.546668 1_2:-4270373 Msa0050380:CDS
GCCGGCTACCCAATCGGAAG+GGG 0.548832 1_2:-4270391 Msa0050380:CDS
GTAAAATTCATAAGCAGAAG+GGG 0.557860 1_2:-4269917 Msa0050380:CDS
AAGTAAAATTCATAAGCAGA+AGG 0.561096 1_2:-4269919 Msa0050380:CDS
TTGATTTCAATTAAATCCTG+AGG 0.568823 1_2:+4270168 None:intergenic
ACGCCGGCTACCCAATCGGA+AGG 0.586765 1_2:-4270393 Msa0050380:CDS
TACTCTGTCGATATTCAGCA+AGG 0.588341 1_2:+4270018 None:intergenic
CGCCGGCTACCCAATCGGAA+GGG 0.588497 1_2:-4270392 Msa0050380:CDS
AAGCAAAATCCAACGTACGC+CGG 0.639196 1_2:-4270409 Msa0050380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGATTTCAATTAAATCCTG+AGG + chr1_2:4270158-4270177 None:intergenic 25.0%
! TCAGGATTTAATTGAAATCA+AGG - chr1_2:4270157-4270176 Msa0050380:intron 25.0%
! AAGTAAAATTCATAAGCAGA+AGG - chr1_2:4270404-4270423 Msa0050380:CDS 25.0%
! AGTAAAATTCATAAGCAGAA+GGG - chr1_2:4270405-4270424 Msa0050380:CDS 25.0%
! AACTCTTTTGGCAAATTCAA+AGG - chr1_2:4269973-4269992 Msa0050380:CDS 30.0%
! ATTCAAAGGCAAGTTTTTCT+CGG - chr1_2:4269987-4270006 Msa0050380:CDS 30.0%
ATATCACTCTTTATACCAAC+AGG - chr1_2:4270064-4270083 Msa0050380:intron 30.0%
ATTAAATCCTGAGGAATGTT+GGG + chr1_2:4270149-4270168 None:intergenic 30.0%
AATTAAATCCTGAGGAATGT+TGG + chr1_2:4270150-4270169 None:intergenic 30.0%
GTAAAATTCATAAGCAGAAG+GGG - chr1_2:4270406-4270425 Msa0050380:CDS 30.0%
TGAATTTGCCAAAAGAGTTC+CGG + chr1_2:4269972-4269991 None:intergenic 35.0%
! TTCAAAGGCAAGTTTTTCTC+GGG - chr1_2:4269988-4270007 Msa0050380:CDS 35.0%
! AAACCAGCAGACGATTATTT+TGG - chr1_2:4270266-4270285 Msa0050380:intron 35.0%
AGAGAATCAGAAGCAAGCAT+GGG + chr1_2:4270020-4270039 None:intergenic 40.0%
GTGACAATTCGTAATCCTGT+TGG + chr1_2:4270082-4270101 None:intergenic 40.0%
CAAGCTTATGATTTCCTCAC+TGG - chr1_2:4270106-4270125 Msa0050380:intron 40.0%
! CTTCAACTCTTTTTCCAGTG+AGG + chr1_2:4270123-4270142 None:intergenic 40.0%
CTCACTGGAAAAAGAGTTGA+AGG - chr1_2:4270121-4270140 Msa0050380:intron 40.0%
TACTCTGTCGATATTCAGCA+AGG + chr1_2:4270308-4270327 None:intergenic 40.0%
AAGCAAAATCCAACGTACGC+CGG - chr1_2:4269914-4269933 Msa0050380:CDS 45.0%
GATTTACTCCCCTTCCGATT+GGG + chr1_2:4269943-4269962 None:intergenic 45.0%
AGGGGAGTAAATCCATTGTC+CGG - chr1_2:4269950-4269969 Msa0050380:CDS 45.0%
! TCCATTGTCCGGAACTCTTT+TGG - chr1_2:4269961-4269980 Msa0050380:CDS 45.0%
CAGAGAATCAGAAGCAAGCA+TGG + chr1_2:4270021-4270040 None:intergenic 45.0%
CCTCCAAAATAATCGTCTGC+TGG + chr1_2:4270272-4270291 None:intergenic 45.0%
!!! CCAGCAGACGATTATTTTGG+AGG - chr1_2:4270269-4270288 Msa0050380:intron 45.0%
CAGCCAAGGCAACTAAAGTT+CGG - chr1_2:4270346-4270365 Msa0050380:intron 45.0%
CTTCCGAACTTTAGTTGCCT+TGG + chr1_2:4270352-4270371 None:intergenic 45.0%
GGATTTACTCCCCTTCCGAT+TGG + chr1_2:4269944-4269963 None:intergenic 50.0%
GCCAAAAGAGTTCCGGACAA+TGG + chr1_2:4269965-4269984 None:intergenic 50.0%
TCAGAAGCAAGCATGGGTAC+AGG + chr1_2:4270014-4270033 None:intergenic 50.0%
GAGTAAAGCTGCTGCAAAGC+AGG - chr1_2:4270323-4270342 Msa0050380:intron 50.0%
! GCAGAAGGGGAAGAAAGTGT+TGG - chr1_2:4270419-4270438 Msa0050380:CDS 50.0%
GAAGGAGCCCAACATTCCTC+AGG - chr1_2:4270139-4270158 Msa0050380:intron 55.0%
ATTGGGTAGCCGGCGTACGT+TGG + chr1_2:4269926-4269945 None:intergenic 60.0%
! ACGTACGCCGGCTACCCAAT+CGG - chr1_2:4269926-4269945 Msa0050380:CDS 60.0%
TCCCCTTCCGATTGGGTAGC+CGG + chr1_2:4269936-4269955 None:intergenic 60.0%
TGCTGCAAAGCAGGCAGCCA+AGG - chr1_2:4270332-4270351 Msa0050380:intron 60.0%
! ACGCCGGCTACCCAATCGGA+AGG - chr1_2:4269930-4269949 Msa0050380:CDS 65.0%
! CGCCGGCTACCCAATCGGAA+GGG - chr1_2:4269931-4269950 Msa0050380:CDS 65.0%
GCCGGCTACCCAATCGGAAG+GGG - chr1_2:4269932-4269951 Msa0050380:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 4269896 4270449 4269896 ID=Msa0050380;Name=Msa0050380
chr1_2 mRNA 4269896 4270449 4269896 ID=Msa0050380-mRNA-1;Parent=Msa0050380;Name=Msa0050380-mRNA-1;_AED=0.39;_eAED=0.40;_QI=0|0|0|1|1|1|3|0|122
chr1_2 exon 4270351 4270449 4270351 ID=Msa0050380-mRNA-1:exon:1074;Parent=Msa0050380-mRNA-1
chr1_2 exon 4270167 4270259 4270167 ID=Msa0050380-mRNA-1:exon:1073;Parent=Msa0050380-mRNA-1
chr1_2 exon 4269896 4270072 4269896 ID=Msa0050380-mRNA-1:exon:1072;Parent=Msa0050380-mRNA-1
chr1_2 CDS 4270351 4270449 4270351 ID=Msa0050380-mRNA-1:cds;Parent=Msa0050380-mRNA-1
chr1_2 CDS 4270167 4270259 4270167 ID=Msa0050380-mRNA-1:cds;Parent=Msa0050380-mRNA-1
chr1_2 CDS 4269896 4270072 4269896 ID=Msa0050380-mRNA-1:cds;Parent=Msa0050380-mRNA-1
Gene Sequence

>Msa0050380

ATGGTTTATTGTAGTGGAAAGCAAAATCCAACGTACGCCGGCTACCCAATCGGAAGGGGAGTAAATCCATTGTCCGGAACTCTTTTGGCAAATTCAAAGGATTACGAATTGTCACAATACAAGCTTATGATTTCCTCACTGGAAAAAGAGTTGAAGGAGCCCAACATTCCTCAGGATTTAATTGAAATCAAGCAGACGATTATTTTGGAGGATTGTCGTGTTCTCCTTGCTGAATATCGACAGAGTAAAGCTGCTGCAAAGCAGGCAGCCAAGGCAACTAAAGTTCGGAAGACAAGTAAAATTCAAAAGAAGACAGCTAAGACAAGTAAAATTCATAAGCAGAAGGGGAAGAAAGTGTTGGAGACTTGA

Protein sequence

>Msa0050380

MVYCSGKQNPTYAGYPIGRGVNPLSGTLLANSKDYELSQYKLMISSLEKELKEPNIPQDLIEIKQTIILEDCRVLLAEYRQSKAAAKQAAKATKVRKTSKIQKKTAKTSKIHKQKGKKVLET*