AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0152220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0152220 MtrunA17_Chr1g0160981 83.200 125 18 3 1 123 1 124 3.81e-67 199
Msa0152220 MtrunA17_Chr8g0391451 67.123 146 35 3 1 146 16 148 5.89e-64 192
Msa0152220 MtrunA17_Chr4g0059791 60.417 96 35 2 50 144 214 307 1.14e-31 114
Msa0152220 MtrunA17_Chr4g0063491 56.180 89 37 1 52 140 154 240 4.48e-29 106
Msa0152220 MtrunA17_Chr8g0360861 43.529 85 46 1 58 142 12 94 3.03e-19 77.4
Msa0152220 MtrunA17_Chr2g0331651 37.778 90 54 1 48 137 2 89 1.38e-18 75.1
Msa0152220 MtrunA17_Chr4g0067531 42.045 88 47 1 52 139 47 130 3.55e-18 75.5
Msa0152220 MtrunA17_Chr6g0485891 37.209 86 52 1 54 139 42 125 1.05e-16 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0152220 AT1G23220.1 58.621 145 43 3 1 145 1 128 3.11e-53 164
Msa0152220 AT5G20110.1 47.244 127 60 2 21 142 81 205 5.28e-36 123
Msa0152220 AT4G27360.1 43.478 92 47 2 58 146 12 101 2.06e-19 78.2
Msa0152220 AT1G52245.1 38.947 95 56 1 48 142 2 94 3.46e-19 77.4
Msa0152220 AT4G15930.1 41.860 86 46 1 54 139 42 123 1.48e-18 76.6
Msa0152220 AT3G16120.2 39.130 92 54 1 48 139 2 91 6.11e-18 73.9
Msa0152220 AT3G16120.1 39.130 92 54 1 48 139 2 91 6.11e-18 73.9

Find 42 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATTTGATGCACTTATATT+TGG 0.148484 1_4:-14790643 Msa0152220:three_prime_UTR
TGCATTGTTGGAACTAGTTT+TGG 0.263515 1_4:-14790868 Msa0152220:CDS
TTTAAATGCAAAATTTCTTC+TGG 0.289949 1_4:-14790597 Msa0152220:three_prime_UTR
GGGTTTGTTTGGTTTGGTTT+TGG 0.304130 1_4:-14793411 Msa0152220:five_prime_UTR
CAAGATGGTCACGTGCAAAT+TGG 0.319181 1_4:+14793148 None:intergenic
TGAGAAGAAAAGGGTTTGTT+TGG 0.342503 1_4:-14793422 Msa0152220:five_prime_UTR
AGGTTCATATCCTTCTCTTC+AGG 0.345046 1_4:-14790795 Msa0152220:CDS
ACTATAAGTTGTGCATCCTT+TGG 0.345376 1_4:-14790734 Msa0152220:three_prime_UTR
CAGGAATTTGACTCTTCATA+TGG 0.355472 1_4:-14790904 Msa0152220:CDS
TCCAATGCTTTGAGAAGAAA+AGG 0.363071 1_4:-14793432 Msa0152220:five_prime_UTR
TTGAAAGAAGAGAGAGAGAA+TGG 0.363479 1_4:+14793476 None:intergenic
AGAAAAGGGTTTGTTTGGTT+TGG 0.374137 1_4:-14793417 Msa0152220:five_prime_UTR
CTATAAGTTGTGCATCCTTT+GGG 0.378685 1_4:-14790733 Msa0152220:three_prime_UTR
GTACAGTGTAATGGAATGAT+AGG 0.385072 1_4:+14793512 None:intergenic
CCAGCTTGGCACTGCATTGT+TGG 0.390226 1_4:-14790880 Msa0152220:CDS
AAGTAGAGTAACCTTCTTAA+GGG 0.392735 1_4:+14793080 None:intergenic
AAAGTAGAGTAACCTTCTTA+AGG 0.404061 1_4:+14793079 None:intergenic
CAGGACAGCTGTTGCACCAT+TGG 0.413092 1_4:-14790776 Msa0152220:CDS
CCAACAATGCAGTGCCAAGC+TGG 0.439774 1_4:+14790880 None:intergenic
GGAAAGCATGTTTCTGCAAA+GGG 0.449689 1_4:+14793169 None:intergenic
TATGTTACTCACTCTGTTGG+TGG 0.459409 1_4:-14790841 Msa0152220:CDS
TTACTGTCAGGCTTCTTAGC+AGG 0.476393 1_4:+14793117 None:intergenic
TGAAAGAAGAGAGAGAGAAT+GGG 0.488141 1_4:+14793477 None:intergenic
AGTGCCAAGTGTTTACTGTC+AGG 0.499308 1_4:+14793105 None:intergenic
AAGGATATGAACCTTATCAA+TGG 0.500156 1_4:+14790804 None:intergenic
TCCTATGTTACTCACTCTGT+TGG 0.508362 1_4:-14790844 Msa0152220:CDS
GAGTAAAATGTACAGTGTAA+TGG 0.514020 1_4:+14793503 None:intergenic
ACTCTTCATATGGCCCAGCT+TGG 0.516405 1_4:-14790894 Msa0152220:CDS
ATAGTTGTGGAAGTTATGAA+TGG 0.517223 1_4:+14790752 None:intergenic
CCAATGCTTTGAGAAGAAAA+GGG 0.521573 1_4:-14793431 Msa0152220:five_prime_UTR
CTTGGCACTCTCCCTTAAGA+AGG 0.524847 1_4:-14793091 Msa0152220:intron
TGGAAAGCATGTTTCTGCAA+AGG 0.534659 1_4:+14793168 None:intergenic
CAACAATGCAGTGCCAAGCT+GGG 0.545687 1_4:+14790881 None:intergenic
TCACATGGAAGAAGCAGAGA+AGG 0.550248 1_4:-14793355 Msa0152220:exon
CAGAGAAGGAGTTAGAGAGA+AGG 0.557565 1_4:-14793341 Msa0152220:CDS
CAACAGCTGTCCTGAAGAGA+AGG 0.560687 1_4:+14790785 None:intergenic
ACCAACAGAGTGAGTAACAT+AGG 0.582734 1_4:+14790843 None:intergenic
GGATGCACAACTTATAGTTG+TGG 0.585937 1_4:+14790739 None:intergenic
TAGCAGGCAAAGAATCAAGA+TGG 0.616352 1_4:+14793133 None:intergenic
GAAGCCTGACAGTAAACACT+TGG 0.632851 1_4:-14793109 Msa0152220:CDS
GGAAGTTATGAATGGTCCAA+TGG 0.665369 1_4:+14790760 None:intergenic
GTGAGTTAAGTAACATCACA+TGG 0.671205 1_4:-14793370 Msa0152220:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAACAATTTTTTTTTTTTT+AGG - chr1_4:14792010-14792029 Msa0152220:intron 10.0%
!! AAATTTCTTCATATTAAAAT+TGG + chr1_4:14792545-14792564 None:intergenic 10.0%
!! TGTATTATACAAAAAAAATT+AGG + chr1_4:14792645-14792664 None:intergenic 10.0%
!!! AATGTTTGATATTTTATGTT+TGG - chr1_4:14791112-14791131 Msa0152220:intron 15.0%
!!! AAGATTCTAAAGAAATATTT+CGG + chr1_4:14792127-14792146 None:intergenic 15.0%
!! AAATTTATTCACATTGAAAT+TGG - chr1_4:14792451-14792470 Msa0152220:intron 15.0%
!! TATACAAATATGATCTTAAA+TGG - chr1_4:14793127-14793146 Msa0152220:CDS 15.0%
!!! TAGAGTTGATTTTAAAAGTT+TGG - chr1_4:14791172-14791191 Msa0152220:intron 20.0%
!!! TGGAGTATGTTTTTTTTTAA+AGG + chr1_4:14791595-14791614 None:intergenic 20.0%
!! TTCTTAAAACATGTTATAAC+AGG + chr1_4:14792236-14792255 None:intergenic 20.0%
!!! TGTTTTAAGAAAAAAGAAAG+AGG - chr1_4:14792245-14792264 Msa0152220:intron 20.0%
!!! TTTAAGCTTTATTAAAAGGA+AGG - chr1_4:14792328-14792347 Msa0152220:intron 20.0%
!!! AAATGTATCAATTTTGCTTT+TGG - chr1_4:14792403-14792422 Msa0152220:intron 20.0%
!!! TTAATATGAAGAAATTTTGC+AGG - chr1_4:14792548-14792567 Msa0152220:intron 20.0%
!!! TAATATGAAGAAATTTTGCA+GGG - chr1_4:14792549-14792568 Msa0152220:intron 20.0%
!! AAATCAATAAATAAGTGTCT+TGG - chr1_4:14792909-14792928 Msa0152220:intron 20.0%
!!! TATGTTGAAAATGTTACTTT+TGG - chr1_4:14792972-14792991 Msa0152220:intron 20.0%
!! ATACATTTAGTAAAAGACAA+AGG + chr1_4:14793031-14793050 None:intergenic 20.0%
!! TTAATGAATTGTACTATCTT+TGG - chr1_4:14793063-14793082 Msa0152220:intron 20.0%
!!! TTTTGAAATTAATTTCCCAA+AGG + chr1_4:14793382-14793401 None:intergenic 20.0%
!!! ATATTTGGATTTTTTGTTTG+TGG - chr1_4:14793469-14793488 Msa0152220:five_prime_UTR 20.0%
!! TTTAAATGCAAAATTTCTTC+TGG - chr1_4:14793500-14793519 Msa0152220:five_prime_UTR 20.0%
!!! TGTTGAAAACTGTTTTAACT+TGG - chr1_4:14791079-14791098 Msa0152220:intron 25.0%
! TTTATGTTTGGTTACACATT+TGG - chr1_4:14791124-14791143 Msa0152220:intron 25.0%
!! ATGAACAGTAAAATTGATTC+CGG + chr1_4:14791337-14791356 None:intergenic 25.0%
!!! GTTTTGTTTTTGCTTTAGTT+GGG + chr1_4:14791520-14791539 None:intergenic 25.0%
!!! AGTTTTGTTTTTGCTTTAGT+TGG + chr1_4:14791521-14791540 None:intergenic 25.0%
! AGCAAAAACAAAACTTTGTA+AGG - chr1_4:14791526-14791545 Msa0152220:intron 25.0%
! AAATGTTGGATATATAAGAG+AGG - chr1_4:14791866-14791885 Msa0152220:intron 25.0%
!!! ATTGTTTGTTGATGTTTTGT+TGG - chr1_4:14792044-14792063 Msa0152220:intron 25.0%
!!! TTGTTTGTTGATGTTTTGTT+GGG - chr1_4:14792045-14792064 Msa0152220:intron 25.0%
! AGAAAGAATATGTAGACTAT+AGG + chr1_4:14792186-14792205 None:intergenic 25.0%
! TATAGTCTACATATTCTTTC+TGG - chr1_4:14792185-14792204 Msa0152220:intron 25.0%
! TAACAGATTCTGCTTTATAT+TGG - chr1_4:14792291-14792310 Msa0152220:intron 25.0%
!! CCTTTTAATAAAGCTTAAAG+AGG + chr1_4:14792327-14792346 None:intergenic 25.0%
!!! CCTCTTTAAGCTTTATTAAA+AGG - chr1_4:14792324-14792343 Msa0152220:intron 25.0%
!!! CCAAAAGTTTTTTTTTTTGC+GGG + chr1_4:14792370-14792389 None:intergenic 25.0%
!!! GCCAAAAGTTTTTTTTTTTG+CGG + chr1_4:14792371-14792390 None:intergenic 25.0%
!! TCAATGTGAATAAATTTTGC+AGG + chr1_4:14792448-14792467 None:intergenic 25.0%
!!! TCATTTTAGTCCTTATTTTG+AGG + chr1_4:14792510-14792529 None:intergenic 25.0%
!!! ACATGAGAATTTTGGTATAA+TGG - chr1_4:14792746-14792765 Msa0152220:intron 25.0%
! TGTTATGTTTGATATCTTTG+TGG - chr1_4:14792782-14792801 Msa0152220:intron 25.0%
! TTACTTCATACTTGAATACA+TGG + chr1_4:14792851-14792870 None:intergenic 25.0%
! TATGTTTATTAGCTGTATGT+TGG - chr1_4:14792881-14792900 Msa0152220:intron 25.0%
!!! TATTCATGCTACTTGTTTTA+GGG + chr1_4:14792942-14792961 None:intergenic 25.0%
!!! ATATTCATGCTACTTGTTTT+AGG + chr1_4:14792943-14792962 None:intergenic 25.0%
! TCTTAAATGGAAAATGACTT+TGG - chr1_4:14793140-14793159 Msa0152220:CDS 25.0%
! AACAATATTAAGAAACTTGC+TGG - chr1_4:14793165-14793184 Msa0152220:CDS 25.0%
! GTATTTGATGCACTTATATT+TGG - chr1_4:14793454-14793473 Msa0152220:five_prime_UTR 25.0%
GAGTAAAATGTACAGTGTAA+TGG + chr1_4:14790597-14790616 None:intergenic 30.0%
AAGTAGAGTAACCTTCTTAA+GGG + chr1_4:14791020-14791039 None:intergenic 30.0%
AAAGTAGAGTAACCTTCTTA+AGG + chr1_4:14791021-14791040 None:intergenic 30.0%
CAACATAACACAACAAATCA+TGG + chr1_4:14791064-14791083 None:intergenic 30.0%
AACTTGAAGCTAGAATTTGA+AGG - chr1_4:14791236-14791255 Msa0152220:intron 30.0%
AAGCTAAAAATCAACTGTAG+AGG + chr1_4:14791267-14791286 None:intergenic 30.0%
ACAAAGAAACGAATTAAGCT+CGG + chr1_4:14791741-14791760 None:intergenic 30.0%
!! ATAGTGTTACTTGAACTTGT+TGG - chr1_4:14791797-14791816 Msa0152220:intron 30.0%
!! TAGTGTTACTTGAACTTGTT+GGG - chr1_4:14791798-14791817 Msa0152220:intron 30.0%
! CCAACATTTTACCTATTCAT+AGG + chr1_4:14791855-14791874 None:intergenic 30.0%
CCTATGAATAGGTAAAATGT+TGG - chr1_4:14791852-14791871 Msa0152220:intron 30.0%
! CTCATATACCTAACGTTTTA+AGG - chr1_4:14791892-14791911 Msa0152220:intron 30.0%
ACTCAAAACCTTAAAACGTT+AGG + chr1_4:14791903-14791922 None:intergenic 30.0%
!!! TAACGTTTTAAGGTTTTGAG+TGG - chr1_4:14791902-14791921 Msa0152220:intron 30.0%
AAGAAGTAACTGCATACATA+AGG + chr1_4:14792083-14792102 None:intergenic 30.0%
ATAAAGCAGAATCTGTTATG+TGG + chr1_4:14792289-14792308 None:intergenic 30.0%
! CCCGCAAAAAAAAAAACTTT+TGG - chr1_4:14792367-14792386 Msa0152220:intron 30.0%
! TTCACATTGAAATTGGTGTT+TGG - chr1_4:14792458-14792477 Msa0152220:intron 30.0%
AGGGACTATTATATATTTGC+AGG - chr1_4:14792568-14792587 Msa0152220:intron 30.0%
! TCATTCACACATGAGAATTT+TGG - chr1_4:14792738-14792757 Msa0152220:intron 30.0%
! TGTGTACTTTTCCATTGATA+AGG - chr1_4:14793282-14793301 Msa0152220:CDS 30.0%
AAGGATATGAACCTTATCAA+TGG + chr1_4:14793296-14793315 None:intergenic 30.0%
ATAGTTGTGGAAGTTATGAA+TGG + chr1_4:14793348-14793367 None:intergenic 30.0%
! GTACAGTGTAATGGAATGAT+AGG + chr1_4:14790588-14790607 None:intergenic 35.0%
TGAAAGAAGAGAGAGAGAAT+GGG + chr1_4:14790623-14790642 None:intergenic 35.0%
TTGAAAGAAGAGAGAGAGAA+TGG + chr1_4:14790624-14790643 None:intergenic 35.0%
! GGAAACTATTTTGCACACTT+TGG + chr1_4:14790648-14790667 None:intergenic 35.0%
!! TCCAATGCTTTGAGAAGAAA+AGG - chr1_4:14790665-14790684 Msa0152220:three_prime_UTR 35.0%
!! CCAATGCTTTGAGAAGAAAA+GGG - chr1_4:14790666-14790685 Msa0152220:three_prime_UTR 35.0%
! TGAGAAGAAAAGGGTTTGTT+TGG - chr1_4:14790675-14790694 Msa0152220:three_prime_UTR 35.0%
! AGAAAAGGGTTTGTTTGGTT+TGG - chr1_4:14790680-14790699 Msa0152220:three_prime_UTR 35.0%
GTGAGTTAAGTAACATCACA+TGG - chr1_4:14790727-14790746 Msa0152220:three_prime_UTR 35.0%
ATGTTTGGTTACACATTTGG+AGG - chr1_4:14791127-14791146 Msa0152220:intron 35.0%
!! AAGGACTAGCCTACATAAAT+TGG - chr1_4:14791545-14791564 Msa0152220:intron 35.0%
ATCATGACTTTGCAACATCA+CGG + chr1_4:14791673-14791692 None:intergenic 35.0%
TTGTTGTGTGAAGTTCTGTT+GGG + chr1_4:14791997-14792016 None:intergenic 35.0%
ATTGTTGTGTGAAGTTCTGT+TGG + chr1_4:14791998-14792017 None:intergenic 35.0%
GATCATAGCTCCTCAAAATA+AGG - chr1_4:14792497-14792516 Msa0152220:intron 35.0%
CATGTGTGAATGAAACAAGT+AGG + chr1_4:14792731-14792750 None:intergenic 35.0%
ATATCTTTGTGGTCCAACAA+AGG - chr1_4:14792793-14792812 Msa0152220:intron 35.0%
AAAAGTGATGCAACCTTTGT+TGG + chr1_4:14792809-14792828 None:intergenic 35.0%
TGGTTTCCTCATTTCACTAA+TGG - chr1_4:14792992-14793011 Msa0152220:intron 35.0%
! ACATTAGTCTCTCCAATTCT+TGG - chr1_4:14793090-14793109 Msa0152220:intron 35.0%
CAGCTTAATTGTCCAAGAAT+TGG + chr1_4:14793105-14793124 None:intergenic 35.0%
CAGGAATTTGACTCTTCATA+TGG - chr1_4:14793193-14793212 Msa0152220:CDS 35.0%
!! TGCATTGTTGGAACTAGTTT+TGG - chr1_4:14793229-14793248 Msa0152220:CDS 35.0%
ACTATAAGTTGTGCATCCTT+TGG - chr1_4:14793363-14793382 Msa0152220:exon 35.0%
CTATAAGTTGTGCATCCTTT+GGG - chr1_4:14793364-14793383 Msa0152220:exon 35.0%
! CCCTTTTCTTCTCAAAGCAT+TGG + chr1_4:14790669-14790688 None:intergenic 40.0%
!!! GGGTTTGTTTGGTTTGGTTT+TGG - chr1_4:14790686-14790705 Msa0152220:three_prime_UTR 40.0%
! GGAAAGCATGTTTCTGCAAA+GGG + chr1_4:14790931-14790950 None:intergenic 40.0%
! TGGAAAGCATGTTTCTGCAA+AGG + chr1_4:14790932-14790951 None:intergenic 40.0%
TAGCAGGCAAAGAATCAAGA+TGG + chr1_4:14790967-14790986 None:intergenic 40.0%
!! GCATTCCTTTCACTTTTAGC+CGG - chr1_4:14791315-14791334 Msa0152220:intron 40.0%
TGATTCCGGCTAAAAGTGAA+AGG + chr1_4:14791323-14791342 None:intergenic 40.0%
TTAGTTGGGCGCTATATTAG+TGG + chr1_4:14791506-14791525 None:intergenic 40.0%
TAGCCTACATAAATTGGCGT+TGG - chr1_4:14791551-14791570 Msa0152220:intron 40.0%
AGCCTACATAAATTGGCGTT+GGG - chr1_4:14791552-14791571 Msa0152220:intron 40.0%
TCTTTCAGAGGTTTGGACTT+TGG + chr1_4:14791615-14791634 None:intergenic 40.0%
GTGTCTTTCTTTCAGAGGTT+TGG + chr1_4:14791622-14791641 None:intergenic 40.0%
! TTGGAGTGTCTTTCTTTCAG+AGG + chr1_4:14791627-14791646 None:intergenic 40.0%
TCTGAAAGAAAGACACTCCA+AGG - chr1_4:14791626-14791645 Msa0152220:intron 40.0%
GGCTCATGTTAAATTGCTCT+GGG + chr1_4:14791652-14791671 None:intergenic 40.0%
GTGATGTTGCAAAGTCATGA+TGG - chr1_4:14791672-14791691 Msa0152220:intron 40.0%
!! AATTAAGCTCGGTGCATGTT+TGG + chr1_4:14791730-14791749 None:intergenic 40.0%
!!! TAAGGTTTTGAGTGGAGATG+TGG - chr1_4:14791910-14791929 Msa0152220:intron 40.0%
CTTCTTCATCCATGTTCTTG+TGG - chr1_4:14792097-14792116 Msa0152220:intron 40.0%
GAAAAAAGAAAGAGGAGAGC+AGG - chr1_4:14792253-14792272 Msa0152220:intron 40.0%
TGCATGCCATTAGTGAAATG+AGG + chr1_4:14793001-14793020 None:intergenic 40.0%
!! AAGAAACTTGCTGGTCTTAC+AGG - chr1_4:14793174-14793193 Msa0152220:CDS 40.0%
TCCTATGTTACTCACTCTGT+TGG - chr1_4:14793253-14793272 Msa0152220:CDS 40.0%
ACCAACAGAGTGAGTAACAT+AGG + chr1_4:14793257-14793276 None:intergenic 40.0%
TATGTTACTCACTCTGTTGG+TGG - chr1_4:14793256-14793275 Msa0152220:CDS 40.0%
AGGTTCATATCCTTCTCTTC+AGG - chr1_4:14793302-14793321 Msa0152220:CDS 40.0%
GGAAGTTATGAATGGTCCAA+TGG + chr1_4:14793340-14793359 None:intergenic 40.0%
GGATGCACAACTTATAGTTG+TGG + chr1_4:14793361-14793380 None:intergenic 40.0%
TCACATGGAAGAAGCAGAGA+AGG - chr1_4:14790742-14790761 Msa0152220:three_prime_UTR 45.0%
CAGAGAAGGAGTTAGAGAGA+AGG - chr1_4:14790756-14790775 Msa0152220:exon 45.0%
CAAGATGGTCACGTGCAAAT+TGG + chr1_4:14790952-14790971 None:intergenic 45.0%
TTACTGTCAGGCTTCTTAGC+AGG + chr1_4:14790983-14791002 None:intergenic 45.0%
GAAGCCTGACAGTAAACACT+TGG - chr1_4:14790988-14791007 Msa0152220:intron 45.0%
AGTGCCAAGTGTTTACTGTC+AGG + chr1_4:14790995-14791014 None:intergenic 45.0%
TTGGGCGCTATATTAGTGGA+CGG + chr1_4:14791502-14791521 None:intergenic 45.0%
CTCCCAACGCCAATTTATGT+AGG + chr1_4:14791557-14791576 None:intergenic 45.0%
! TGTTAAATTGCTCTGGGCCT+TGG + chr1_4:14791646-14791665 None:intergenic 45.0%
CGGCTCATGTTAAATTGCTC+TGG + chr1_4:14791653-14791672 None:intergenic 45.0%
! TGTTGGGTTTCAAGTGTGAG+CGG - chr1_4:14791814-14791833 Msa0152220:intron 45.0%
GTCTCACATCGCCTATGAAT+AGG - chr1_4:14791841-14791860 Msa0152220:intron 45.0%
TGAAGTTCTGTTGGGTTCGA+GGG + chr1_4:14791989-14792008 None:intergenic 45.0%
CTTGGCACTCTCCCTTAAGA+AGG - chr1_4:14791006-14791025 Msa0152220:intron 50.0%
TGCTCCAGGACCACAATAGA+GGG + chr1_4:14791941-14791960 None:intergenic 50.0%
ATGCTCCAGGACCACAATAG+AGG + chr1_4:14791942-14791961 None:intergenic 50.0%
CAACAACAGGTCAATGCTCC+AGG + chr1_4:14791955-14791974 None:intergenic 50.0%
GTGAAGTTCTGTTGGGTTCG+AGG + chr1_4:14791990-14792009 None:intergenic 50.0%
TTCGGCACACCACAAGAACA+TGG + chr1_4:14792109-14792128 None:intergenic 50.0%
ACTCTTCATATGGCCCAGCT+TGG - chr1_4:14793203-14793222 Msa0152220:CDS 50.0%
CAACAATGCAGTGCCAAGCT+GGG + chr1_4:14793219-14793238 None:intergenic 50.0%
CAACAGCTGTCCTGAAGAGA+AGG + chr1_4:14793315-14793334 None:intergenic 50.0%
TGTGGCGTCTCCCTCTATTG+TGG - chr1_4:14791928-14791947 Msa0152220:intron 55.0%
GTCTCCCTCTATTGTGGTCC+TGG - chr1_4:14791934-14791953 Msa0152220:intron 55.0%
!! GGAGTGTCGGGAACAACAAC+AGG + chr1_4:14791968-14791987 None:intergenic 55.0%
! TGTTGGGTTCGAGGGAGTGT+CGG + chr1_4:14791981-14792000 None:intergenic 55.0%
CCAACAATGCAGTGCCAAGC+TGG + chr1_4:14793220-14793239 None:intergenic 55.0%
CCAGCTTGGCACTGCATTGT+TGG - chr1_4:14793217-14793236 Msa0152220:CDS 55.0%
CAGGACAGCTGTTGCACCAT+TGG - chr1_4:14793321-14793340 Msa0152220:CDS 55.0%
!! GTTGGGTTCGAGGGAGTGTC+GGG + chr1_4:14791980-14791999 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 14790578 14793541 14790578 ID=Msa0152220;Name=Msa0152220
chr1_4 mRNA 14790578 14793541 14790578 ID=Msa0152220-mRNA-1;Parent=Msa0152220;Name=Msa0152220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=168|1|1|1|1|1|2|187|146
chr1_4 exon 14790578 14790923 14790578 ID=Msa0152220-mRNA-1:exon:5824;Parent=Msa0152220-mRNA-1
chr1_4 exon 14793092 14793541 14793092 ID=Msa0152220-mRNA-1:exon:5823;Parent=Msa0152220-mRNA-1
chr1_4 five_prime_UTR 14793374 14793541 14793374 ID=Msa0152220-mRNA-1:five_prime_utr;Parent=Msa0152220-mRNA-1
chr1_4 CDS 14793092 14793373 14793092 ID=Msa0152220-mRNA-1:cds;Parent=Msa0152220-mRNA-1
chr1_4 CDS 14790765 14790923 14790765 ID=Msa0152220-mRNA-1:cds;Parent=Msa0152220-mRNA-1
chr1_4 three_prime_UTR 14790578 14790764 14790578 ID=Msa0152220-mRNA-1:three_prime_utr;Parent=Msa0152220-mRNA-1
Gene Sequence

>Msa0152220

ATGGAAGAAGCAGAGAAGGAGTTAGAGAGAAGGAGTAAGTTTCTAAACAGTTTGATACAGAACAAGAAGAAGAAATCAACAGAACAAGAACAAAAACAAGAGAAAGAACAAGAACATGATGATAAAAATAATAATATTAAACTCAACAACAGCATTCATGTCAAAGCTTGTGACATGTCTCTCCCTTTGCAGAAACATGCTTTCCAATTTGCACGTGACCATCTTGATTCTTTGCCTGCTAAGAAGCCTGACAGTAAACACTTGGCACTCTCCCTTAAGAAGGAATTTGACTCTTCATATGGCCCAGCTTGGCACTGCATTGTTGGAACTAGTTTTGGTTCCTATGTTACTCACTCTGTTGGTGGTTTTGTGTACTTTTCCATTGATAAGGTTCATATCCTTCTCTTCAGGACAGCTGTTGCACCATTGGACCATTCATAA
Protein sequence

>Msa0152220

MEEAEKELERRSKFLNSLIQNKKKKSTEQEQKQEKEQEHDDKNNNIKLNNSIHVKACDMSLPLQKHAFQFARDHLDSLPAKKPDSKHLALSLKKEFDSSYGPAWHCIVGTSFGSYVTHSVGGFVYFSIDKVHILLFRTAVAPLDHS*