Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0967170 | sp|Q9LVB0|AHL6_ARATH | 54.545 | 220 | 85 | 6 | 50 | 257 | 75 | 291 | 2.35e-59 | 198 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0967170 | A0A396HFC3 | 98.746 | 319 | 4 | 0 | 1 | 319 | 1 | 319 | 0.0 | 632 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0038290 | Msa0967170 | -0.818607 | 1.794446e-52 | -8.615850e-47 |
| Msa0057770 | Msa0967170 | -0.802330 | 5.905599e-49 | -8.615850e-47 |
| Msa0081780 | Msa0967170 | 0.802399 | 5.716106e-49 | -8.615850e-47 |
| Msa0093950 | Msa0967170 | -0.815513 | 8.897590e-52 | -8.615850e-47 |
| Msa0126000 | Msa0967170 | -0.804909 | 1.722956e-49 | -8.615850e-47 |
| Msa0133140 | Msa0967170 | -0.800808 | 1.211532e-48 | -8.615850e-47 |
| Msa0152220 | Msa0967170 | 0.834203 | 3.432684e-56 | -8.615850e-47 |
| Msa0168310 | Msa0967170 | 0.828298 | 9.716215e-55 | -8.615850e-47 |
| Msa0168410 | Msa0967170 | -0.852431 | 4.637854e-61 | -8.615850e-47 |
| Msa0220400 | Msa0967170 | -0.826380 | 2.800469e-54 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0967170 | MtrunA17_Chr6g0476061 | 98.746 | 319 | 4 | 0 | 1 | 319 | 1 | 319 | 0.0 | 632 |
| Msa0967170 | MtrunA17_Chr1g0188211 | 53.353 | 343 | 116 | 13 | 1 | 317 | 1 | 325 | 1.33e-87 | 266 |
| Msa0967170 | MtrunA17_Chr7g0245841 | 41.954 | 348 | 154 | 10 | 1 | 319 | 1 | 329 | 3.23e-51 | 172 |
| Msa0967170 | MtrunA17_Chr6g0476241 | 45.495 | 222 | 89 | 7 | 43 | 260 | 112 | 305 | 1.41e-43 | 151 |
| Msa0967170 | MtrunA17_Chr4g0056761 | 52.232 | 224 | 88 | 7 | 43 | 258 | 64 | 276 | 1.05e-42 | 150 |
| Msa0967170 | MtrunA17_Chr8g0351761 | 47.234 | 235 | 98 | 7 | 40 | 263 | 60 | 279 | 1.09e-39 | 142 |
| Msa0967170 | MtrunA17_Chr1g0179461 | 41.026 | 234 | 108 | 6 | 51 | 269 | 69 | 287 | 1.29e-35 | 132 |
| Msa0967170 | MtrunA17_Chr4g0054661 | 39.407 | 236 | 111 | 6 | 51 | 268 | 73 | 294 | 1.18e-32 | 124 |
| Msa0967170 | MtrunA17_Chr5g0397551 | 36.029 | 272 | 139 | 8 | 1 | 253 | 1 | 256 | 4.61e-30 | 116 |
| Msa0967170 | MtrunA17_Chr7g0247431 | 37.931 | 232 | 126 | 5 | 29 | 260 | 74 | 287 | 6.23e-29 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0967170 | AT5G62260.1 | 54.545 | 220 | 85 | 6 | 50 | 257 | 75 | 291 | 2.39e-60 | 198 |
| Msa0967170 | AT5G62260.3 | 54.545 | 220 | 85 | 6 | 50 | 257 | 75 | 291 | 2.39e-60 | 198 |
| Msa0967170 | AT5G62260.2 | 55.072 | 207 | 82 | 5 | 50 | 248 | 75 | 278 | 1.33e-58 | 190 |
| Msa0967170 | AT4G25320.1 | 48.000 | 250 | 119 | 5 | 38 | 281 | 73 | 317 | 1.79e-57 | 191 |
| Msa0967170 | AT5G51590.1 | 52.018 | 223 | 78 | 5 | 49 | 246 | 76 | 294 | 2.93e-51 | 175 |
| Msa0967170 | AT4G12080.1 | 49.784 | 231 | 92 | 8 | 50 | 264 | 88 | 310 | 9.54e-51 | 172 |
| Msa0967170 | AT4G00200.2 | 48.918 | 231 | 101 | 5 | 38 | 264 | 43 | 260 | 2.61e-47 | 162 |
| Msa0967170 | AT4G00200.1 | 48.918 | 231 | 101 | 5 | 38 | 264 | 43 | 260 | 2.61e-47 | 162 |
| Msa0967170 | AT2G33620.1 | 47.248 | 218 | 98 | 5 | 50 | 265 | 96 | 298 | 2.91e-42 | 149 |
| Msa0967170 | AT2G33620.4 | 47.248 | 218 | 98 | 5 | 50 | 265 | 96 | 298 | 2.91e-42 | 149 |
Find 97 sgRNAs with CRISPR-Local
Find 408 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTGGTGGTGGTGGAGG+TGG | 0.408449 | 6_3:-96440308 | Msa0967170:CDS |
| AAAATCTCCGGTCAACGGAA+TGG | 0.558235 | 6_3:+96440340 | None:intergenic |
| AAACCGGCCGGTGACATGAT+AGG | 0.454468 | 6_3:+96435407 | None:intergenic |
| AACGGAATGGAAGCCGAAAT+TGG | 0.321360 | 6_3:+96440353 | None:intergenic |
| AACTTTGCTTCTTTCGGCAA+TGG | 0.426973 | 6_3:-96435383 | Msa0967170:CDS |
| AAGAGTGATTTATGAGAATT+AGG | 0.142788 | 6_3:+96440944 | None:intergenic |
| AATATCAGTTCTCAATTCTT+CGG | 0.403456 | 6_3:+96435442 | None:intergenic |
| AATATGAGATGATGTAGGAG+GGG | 0.606351 | 6_3:+96435481 | None:intergenic |
| AATGAAGCCTATCATGTCAC+CGG | 0.606781 | 6_3:-96435414 | Msa0967170:CDS |
| AATGTTAACCAGGCCCCACC+TGG | 0.534870 | 6_3:-96440527 | Msa0967170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTAACTAACCAGATCAA+AGG | + | chr6_3:96435791-96435810 | None:intergenic | 30.0% | |
| TTTGATTCATGGTTTAGAAG+TGG | - | chr6_3:96435935-96435954 | Msa0967170:intron | 30.0% | |
| CAGATCTTAAATTGAAGATG+TGG | - | chr6_3:96435989-96436008 | Msa0967170:intron | 30.0% | |
| GATCTTAAATTGAAGATGTG+GGG | - | chr6_3:96435991-96436010 | Msa0967170:intron | 30.0% | |
| GGACTCAAAATAGTATAATC+TGG | - | chr6_3:96436499-96436518 | Msa0967170:CDS | 30.0% | |
| TTCTTCATTGCAGAATGTAA+TGG | - | chr6_3:96436623-96436642 | Msa0967170:intron | 30.0% | |
| ATATGCATAAAGACAAAACC+AGG | + | chr6_3:96436738-96436757 | None:intergenic | 30.0% | |
| ATATGATGATTTAGTGAAGC+AGG | - | chr6_3:96436754-96436773 | Msa0967170:intron | 30.0% | |
| TATGATGATTTAGTGAAGCA+GGG | - | chr6_3:96436755-96436774 | Msa0967170:intron | 30.0% | |
| CCATTATCTACAAAATAGAC+AGG | + | chr6_3:96436932-96436951 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 96435006 | 96440973 | 96435006 | ID=Msa0967170;Name=Msa0967170 |
| chr6_3 | mRNA | 96435006 | 96440973 | 96435006 | ID=Msa0967170-mRNA-1;Parent=Msa0967170;Name=Msa0967170-mRNA-1;_AED=0.17;_eAED=0.17;_QI=385|1|1|1|1|1|5|328|319 |
| chr6_3 | exon | 96435006 | 96435576 | 96435006 | ID=Msa0967170-mRNA-1:exon:17869;Parent=Msa0967170-mRNA-1 |
| chr6_3 | exon | 96436421 | 96436582 | 96436421 | ID=Msa0967170-mRNA-1:exon:17868;Parent=Msa0967170-mRNA-1 |
| chr6_3 | exon | 96437394 | 96437525 | 96437394 | ID=Msa0967170-mRNA-1:exon:17867;Parent=Msa0967170-mRNA-1 |
| chr6_3 | exon | 96438970 | 96439034 | 96438970 | ID=Msa0967170-mRNA-1:exon:17866;Parent=Msa0967170-mRNA-1 |
| chr6_3 | exon | 96440231 | 96440973 | 96440231 | ID=Msa0967170-mRNA-1:exon:17865;Parent=Msa0967170-mRNA-1 |
| chr6_3 | five_prime_UTR | 96440589 | 96440973 | 96440589 | ID=Msa0967170-mRNA-1:five_prime_utr;Parent=Msa0967170-mRNA-1 |
| chr6_3 | CDS | 96440231 | 96440588 | 96440231 | ID=Msa0967170-mRNA-1:cds;Parent=Msa0967170-mRNA-1 |
| chr6_3 | CDS | 96438970 | 96439034 | 96438970 | ID=Msa0967170-mRNA-1:cds;Parent=Msa0967170-mRNA-1 |
| chr6_3 | CDS | 96437394 | 96437525 | 96437394 | ID=Msa0967170-mRNA-1:cds;Parent=Msa0967170-mRNA-1 |
| chr6_3 | CDS | 96436421 | 96436582 | 96436421 | ID=Msa0967170-mRNA-1:cds;Parent=Msa0967170-mRNA-1 |
| chr6_3 | CDS | 96435334 | 96435576 | 96435334 | ID=Msa0967170-mRNA-1:cds;Parent=Msa0967170-mRNA-1 |
| chr6_3 | three_prime_UTR | 96435006 | 96435333 | 96435006 | ID=Msa0967170-mRNA-1:three_prime_utr;Parent=Msa0967170-mRNA-1 |
| Gene Sequence |
>Msa0967170 ATGGAAGAAAGAGAGATTTTTGGTTCTGGGCATGCTGTTAATGTTAACCAGGCCCCACCTGGCTTCAATTTGGCTCAAAATACGTTGAATTTTGCTGGGTCCACTGGAGAGTTACCGGCTCCGGTGCCGGCTGGCGGTGGTGTGGAGGTGAAGAAGAAGAGGGGCAGGCCTAGAAAGAGTGAATCTGGAAGTAAGCCGGCGTTGTCACCGATGCCAATTTCGGCTTCCATTCCGTTGACCGGAGATTTTTCAGGTTGGAAAAGTGGTGGTGGTGGAGGTGGAGGTGTAGTGAAGCCATTTGAATCAATTAAGAAGCCTTTGAAGTTGAATGATTTTGATGAAGGTAATTTTGATGAAGATAATGGAATATCACCCTTCGATTCCAATTTCAAAACGCATGTGCTTACTGTAAATTCTGGAGAGGATGTCTCGATGAAAATAATGTCTCTTTCTCAACAAGAATATCATACTATATCTATTCTGTCTGCGACTGGCACAATTTCGAATGTAACACTTCGCCAGTCGGATGCTTGTGGCGGTACTTCAACGTATGAGGGAGTTTTTGAGATTCTTTCCTTGAGCGGCTCATTTGTGCCAACTGAGAATGGATTGACAAAGAGCAGATCTGGTAGAATGAGTGTCTCATTGGCAGGTCCAAATGGTCGTGTTTTTGGGGGTGCACTGGCTGGTTTGCTGGTAGCTGCTGGTTCTGTGCAGGTTGTGGTTGCGAGTTTTTTTCCAGAGAAGGAGAATCCAAAGAGGCAAAGGGTCGATCATTCGGCACCAACTGCCCCTCCTACATCATCTCATATTAATAATCATGTTTCTTCCGAAGAATTGAGAACTGATATTGGTGGAATGAAGCCTATCATGTCACCGGCCGGTTTTAACTTTGCTTCTTTCGGCAATGGCCAAGGCTCTGGAAACTCATCATCATCTGCGGATGATGAGCATGTCTAA |
| Protein sequence |
>Msa0967170 MEEREIFGSGHAVNVNQAPPGFNLAQNTLNFAGSTGELPAPVPAGGGVEVKKKRGRPRKSESGSKPALSPMPISASIPLTGDFSGWKSGGGGGGGVVKPFESIKKPLKLNDFDEGNFDEDNGISPFDSNFKTHVLTVNSGEDVSMKIMSLSQQEYHTISILSATGTISNVTLRQSDACGGTSTYEGVFEILSLSGSFVPTENGLTKSRSGRMSVSLAGPNGRVFGGALAGLLVAAGSVQVVVASFFPEKENPKRQRVDHSAPTAPPTSSHINNHVSSEELRTDIGGMKPIMSPAGFNFASFGNGQGSGNSSSSADDEHV* |
