Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372770 | XP_013453279.2 | 91.981 | 212 | 10 | 2 | 1 | 212 | 1 | 205 | 1.15e-110 | 351 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372770 | sp|Q8VY06|PREP2_ARATH | 61.111 | 180 | 55 | 6 | 42 | 212 | 19 | 192 | 4.41e-51 | 180 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372770 | A0A396HIP5 | 91.981 | 212 | 10 | 2 | 1 | 212 | 1 | 205 | 5.47e-111 | 351 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001550 | Msa1372770 | 0.821531 | 3.842385e-53 | -8.615850e-47 |
| Msa0002110 | Msa1372770 | 0.831539 | 1.576517e-55 | -8.615850e-47 |
| Msa0007810 | Msa1372770 | 0.813420 | 2.582643e-51 | -8.615850e-47 |
| Msa0011410 | Msa1372770 | 0.813168 | 2.933488e-51 | -8.615850e-47 |
| Msa0011440 | Msa1372770 | 0.823133 | 1.631807e-53 | -8.615850e-47 |
| Msa0011450 | Msa1372770 | 0.824325 | 8.582521e-54 | -8.615850e-47 |
| Msa0029520 | Msa1372770 | 0.847849 | 8.914400e-60 | -8.615850e-47 |
| Msa0035050 | Msa1372770 | 0.848006 | 8.066579e-60 | -8.615850e-47 |
| Msa0040470 | Msa1372770 | -0.808618 | 2.834185e-50 | -8.615850e-47 |
| Msa0042760 | Msa1372770 | 0.840257 | 9.697134e-58 | -8.615850e-47 |
| Msa0046020 | Msa1372770 | 0.801921 | 7.165612e-49 | -8.615850e-47 |
| Msa0056230 | Msa1372770 | 0.805974 | 1.029954e-49 | -8.615850e-47 |
| Msa0066890 | Msa1372770 | 0.876693 | 1.139983e-68 | -8.615850e-47 |
| Msa0067370 | Msa1372770 | 0.817454 | 3.269810e-52 | -8.615850e-47 |
| Msa0067590 | Msa1372770 | 0.853688 | 2.025834e-61 | -8.615850e-47 |
| Msa0081440 | Msa1372770 | 0.807290 | 5.432023e-50 | -8.615850e-47 |
| Msa0081610 | Msa1372770 | 0.803848 | 2.865324e-49 | -8.615850e-47 |
| Msa0093950 | Msa1372770 | 0.849730 | 2.679771e-60 | -8.615850e-47 |
| Msa0094170 | Msa1372770 | 0.842031 | 3.313444e-58 | -8.615850e-47 |
| Msa0095810 | Msa1372770 | 0.840734 | 7.273794e-58 | -8.615850e-47 |
| Msa0111110 | Msa1372770 | 0.845416 | 4.118481e-59 | -8.615850e-47 |
| Msa0112880 | Msa1372770 | 0.844509 | 7.236062e-59 | -8.615850e-47 |
| Msa0113370 | Msa1372770 | 0.820739 | 5.851107e-53 | -8.615850e-47 |
| Msa1352600 | Msa1372770 | 0.809598 | 1.748406e-50 | -8.615850e-47 |
| Msa1360070 | Msa1372770 | 0.822650 | 2.115168e-53 | -8.615850e-47 |
| Msa1361670 | Msa1372770 | 0.822993 | 1.759035e-53 | -8.615850e-47 |
| Msa1364350 | Msa1372770 | 0.844302 | 8.223096e-59 | -8.615850e-47 |
| Msa1372770 | Msa1373420 | 0.802202 | 6.273449e-49 | -8.615850e-47 |
| Msa1372770 | Msa1375610 | 0.832260 | 1.045978e-55 | -8.615850e-47 |
| Msa1372770 | Msa1376550 | 0.826641 | 2.426945e-54 | -8.615850e-47 |
| Msa1372770 | Msa1381340 | 0.806205 | 9.211152e-50 | -8.615850e-47 |
| Msa1372770 | Msa1382380 | 0.824303 | 8.682567e-54 | -8.615850e-47 |
| Msa1372770 | Msa1388030 | 0.824914 | 6.234221e-54 | -8.615850e-47 |
| Msa1372770 | Msa1409360 | 0.819425 | 1.169129e-52 | -8.615850e-47 |
| Msa1372770 | Msa1431510 | 0.823791 | 1.145375e-53 | -8.615850e-47 |
| Msa1372770 | Msa1440990 | 0.825665 | 4.142123e-54 | -8.615850e-47 |
| Msa1372770 | Msa1452230 | 0.814663 | 1.373495e-51 | -8.615850e-47 |
| Msa1372770 | Msa1457390 | 0.830530 | 2.788431e-55 | -8.615850e-47 |
| Msa1372770 | Msa1459130 | 0.814748 | 1.315638e-51 | -8.615850e-47 |
| Msa1372770 | Msa1462210 | 0.823204 | 1.571116e-53 | -8.615850e-47 |
| Msa0603690 | Msa1372770 | 0.809569 | 1.773637e-50 | -8.615850e-47 |
| Msa0605040 | Msa1372770 | 0.813280 | 2.771898e-51 | -8.615850e-47 |
| Msa0605160 | Msa1372770 | 0.812619 | 3.869828e-51 | -8.615850e-47 |
| Msa0637930 | Msa1372770 | 0.841098 | 5.837904e-58 | -8.615850e-47 |
| Msa0638010 | Msa1372770 | 0.865232 | 6.856743e-65 | -8.615850e-47 |
| Msa0646410 | Msa1372770 | 0.815929 | 7.185536e-52 | -8.615850e-47 |
| Msa0676570 | Msa1372770 | 0.821080 | 4.882119e-53 | -8.615850e-47 |
| Msa0686950 | Msa1372770 | 0.863679 | 2.095822e-64 | -8.615850e-47 |
| Msa0701800 | Msa1372770 | 0.811807 | 5.820289e-51 | -8.615850e-47 |
| Msa0742370 | Msa1372770 | 0.821219 | 4.536093e-53 | -8.615850e-47 |
| Msa0752490 | Msa1372770 | 0.818008 | 2.451836e-52 | -8.615850e-47 |
| Msa0779450 | Msa1372770 | 0.828549 | 8.452643e-55 | -8.615850e-47 |
| Msa0793130 | Msa1372770 | 0.801351 | 9.381552e-49 | -8.615850e-47 |
| Msa0823480 | Msa1372770 | 0.819063 | 1.414059e-52 | -8.615850e-47 |
| Msa0827520 | Msa1372770 | 0.860918 | 1.477049e-63 | -8.615850e-47 |
| Msa0833950 | Msa1372770 | 0.813346 | 2.681591e-51 | -8.615850e-47 |
| Msa0120880 | Msa1372770 | -0.803731 | 3.030925e-49 | -8.615850e-47 |
| Msa0133140 | Msa1372770 | 0.824661 | 7.152745e-54 | -8.615850e-47 |
| Msa0136870 | Msa1372770 | 0.839538 | 1.492853e-57 | -8.615850e-47 |
| Msa0140770 | Msa1372770 | 0.836072 | 1.159557e-56 | -8.615850e-47 |
| Msa0151380 | Msa1372770 | 0.832985 | 6.916223e-56 | -8.615850e-47 |
| Msa0151510 | Msa1372770 | 0.827522 | 1.493178e-54 | -8.615850e-47 |
| Msa0152220 | Msa1372770 | -0.818164 | 2.261368e-52 | -8.615850e-47 |
| Msa0154880 | Msa1372770 | 0.873001 | 2.060869e-67 | -8.615850e-47 |
| Msa0154890 | Msa1372770 | 0.856253 | 3.645048e-62 | -8.615850e-47 |
| Msa0168770 | Msa1372770 | 0.811027 | 8.594983e-51 | -8.615850e-47 |
| Msa0178840 | Msa1372770 | 0.826709 | 2.337918e-54 | -8.615850e-47 |
| Msa0187590 | Msa1372770 | 0.815002 | 1.155387e-51 | -8.615850e-47 |
| Msa0189560 | Msa1372770 | 0.807744 | 4.352555e-50 | -8.615850e-47 |
| Msa0220400 | Msa1372770 | 0.821520 | 3.865620e-53 | -8.615850e-47 |
| Msa0225790 | Msa1372770 | 0.822335 | 2.503328e-53 | -8.615850e-47 |
| Msa1255480 | Msa1372770 | 0.805275 | 1.443854e-49 | -8.615850e-47 |
| Msa1258030 | Msa1372770 | 0.834502 | 2.887792e-56 | -8.615850e-47 |
| Msa1293040 | Msa1372770 | 0.825943 | 3.557679e-54 | -8.615850e-47 |
| Msa1333220 | Msa1372770 | 0.845091 | 5.042261e-59 | -8.615850e-47 |
| Msa1341060 | Msa1372770 | -0.824607 | 7.365969e-54 | -8.615850e-47 |
| Msa1342960 | Msa1372770 | 0.838045 | 3.630282e-57 | -8.615850e-47 |
| Msa0367540 | Msa1372770 | 0.812964 | 3.251861e-51 | -8.615850e-47 |
| Msa0370580 | Msa1372770 | 0.849050 | 4.145639e-60 | -8.615850e-47 |
| Msa0373120 | Msa1372770 | 0.839934 | 1.176968e-57 | -8.615850e-47 |
| Msa0375330 | Msa1372770 | 0.804578 | 2.020095e-49 | -8.615850e-47 |
| Msa0380400 | Msa1372770 | 0.813265 | 2.793161e-51 | -8.615850e-47 |
| Msa0383870 | Msa1372770 | 0.840517 | 8.288708e-58 | -8.615850e-47 |
| Msa0385930 | Msa1372770 | 0.873836 | 1.079314e-67 | -8.615850e-47 |
| Msa0388790 | Msa1372770 | 0.804712 | 1.893427e-49 | -8.615850e-47 |
| Msa0415160 | Msa1372770 | 0.837354 | 5.462714e-57 | -8.615850e-47 |
| Msa0437820 | Msa1372770 | 0.801061 | 1.075335e-48 | -8.615850e-47 |
| Msa0460170 | Msa1372770 | 0.805659 | 1.199431e-49 | -8.615850e-47 |
| Msa0476170 | Msa1372770 | 0.827203 | 1.781533e-54 | -8.615850e-47 |
| Msa0479760 | Msa1372770 | 0.842577 | 2.375763e-58 | -8.615850e-47 |
| Msa0480090 | Msa1372770 | 0.827930 | 1.191424e-54 | -8.615850e-47 |
| Msa0982090 | Msa1372770 | 0.826539 | 2.566804e-54 | -8.615850e-47 |
| Msa1004410 | Msa1372770 | 0.820321 | 7.296828e-53 | -8.615850e-47 |
| Msa1011140 | Msa1372770 | 0.802428 | 5.637494e-49 | -8.615850e-47 |
| Msa1020210 | Msa1372770 | -0.812740 | 3.641129e-51 | -8.615850e-47 |
| Msa1028860 | Msa1372770 | 0.803605 | 3.218345e-49 | -8.615850e-47 |
| Msa1039190 | Msa1372770 | 0.845183 | 4.761878e-59 | -8.615850e-47 |
| Msa1039440 | Msa1372770 | 0.839272 | 1.749939e-57 | -8.615850e-47 |
| Msa1067980 | Msa1372770 | 0.805619 | 1.223023e-49 | -8.615850e-47 |
| Msa1083620 | Msa1372770 | 0.802245 | 6.147432e-49 | -8.615850e-47 |
| Msa1085610 | Msa1372770 | 0.852086 | 5.815271e-61 | -8.615850e-47 |
| Msa1086510 | Msa1372770 | 0.800597 | 1.337468e-48 | -8.615850e-47 |
| Msa1087520 | Msa1372770 | 0.821893 | 3.169630e-53 | -8.615850e-47 |
| Msa0260580 | Msa1372770 | 0.839661 | 1.386514e-57 | -8.615850e-47 |
| Msa0260640 | Msa1372770 | 0.818838 | 1.590119e-52 | -8.615850e-47 |
| Msa0262350 | Msa1372770 | 0.800658 | 1.299695e-48 | -8.615850e-47 |
| Msa0268450 | Msa1372770 | 0.800796 | 1.218423e-48 | -8.615850e-47 |
| Msa0268670 | Msa1372770 | 0.807599 | 4.672972e-50 | -8.615850e-47 |
| Msa0268880 | Msa1372770 | 0.803885 | 2.816217e-49 | -8.615850e-47 |
| Msa0275250 | Msa1372770 | 0.800948 | 1.134117e-48 | -8.615850e-47 |
| Msa0297610 | Msa1372770 | -0.803518 | 3.354711e-49 | -8.615850e-47 |
| Msa0326630 | Msa1372770 | 0.831409 | 1.696826e-55 | -8.615850e-47 |
| Msa0327990 | Msa1372770 | 0.804829 | 1.790594e-49 | -8.615850e-47 |
| Msa1128520 | Msa1372770 | 0.800015 | 1.757407e-48 | -8.615850e-47 |
| Msa1128610 | Msa1372770 | 0.824705 | 6.983703e-54 | -8.615850e-47 |
| Msa1129300 | Msa1372770 | 0.833462 | 5.259953e-56 | -8.615850e-47 |
| Msa1207920 | Msa1372770 | 0.810857 | 9.355436e-51 | -8.615850e-47 |
| Msa1219530 | Msa1372770 | 0.821462 | 3.986564e-53 | -8.615850e-47 |
| Msa0500610 | Msa1372770 | 0.811342 | 7.344516e-51 | -8.615850e-47 |
| Msa0523420 | Msa1372770 | 0.815046 | 1.129652e-51 | -8.615850e-47 |
| Msa0523430 | Msa1372770 | 0.853132 | 2.924130e-61 | -8.615850e-47 |
| Msa0523490 | Msa1372770 | 0.822619 | 2.149690e-53 | -8.615850e-47 |
| Msa0548650 | Msa1372770 | 0.844087 | 9.394046e-59 | -8.615850e-47 |
| Msa0550990 | Msa1372770 | 0.800943 | 1.136943e-48 | -8.615850e-47 |
| Msa0566940 | Msa1372770 | 0.801848 | 7.417499e-49 | -8.615850e-47 |
| Msa0576020 | Msa1372770 | 0.812450 | 4.212960e-51 | -8.615850e-47 |
| Msa0576080 | Msa1372770 | 0.839413 | 1.608459e-57 | -8.615850e-47 |
| Msa0576090 | Msa1372770 | 0.823915 | 1.070832e-53 | -8.615850e-47 |
| Msa0576120 | Msa1372770 | 0.839329 | 1.691674e-57 | -8.615850e-47 |
| Msa0582860 | Msa1372770 | 0.805636 | 1.212912e-49 | -8.615850e-47 |
| Msa0590620 | Msa1372770 | 0.827493 | 1.517266e-54 | -8.615850e-47 |
| Msa0862480 | Msa1372770 | 0.822695 | 2.064411e-53 | -8.615850e-47 |
| Msa0866060 | Msa1372770 | 0.807052 | 6.100613e-50 | -8.615850e-47 |
| Msa0881530 | Msa1372770 | 0.849698 | 2.736799e-60 | -8.615850e-47 |
| Msa0881880 | Msa1372770 | 0.823092 | 1.668650e-53 | -8.615850e-47 |
| Msa0919120 | Msa1372770 | 0.835073 | 2.074805e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372770 | MtrunA17_Chr5g0393451 | 91.981 | 212 | 10 | 2 | 1 | 212 | 1 | 205 | 1.05e-114 | 351 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372770 | AT1G49630.3 | 61.111 | 180 | 55 | 6 | 42 | 212 | 19 | 192 | 4.49e-52 | 180 |
| Msa1372770 | AT1G49630.1 | 61.111 | 180 | 55 | 6 | 42 | 212 | 19 | 192 | 4.49e-52 | 180 |
| Msa1372770 | AT1G49630.2 | 61.111 | 180 | 55 | 6 | 42 | 212 | 19 | 192 | 4.49e-52 | 180 |
| Msa1372770 | AT3G19170.2 | 80.952 | 105 | 14 | 1 | 108 | 212 | 95 | 193 | 1.20e-51 | 179 |
| Msa1372770 | AT3G19170.1 | 80.952 | 105 | 14 | 1 | 108 | 212 | 95 | 193 | 1.58e-51 | 178 |
| Msa1372770 | AT1G49630.5 | 97.368 | 38 | 1 | 0 | 175 | 212 | 7 | 44 | 1.08e-18 | 84.3 |
Find 55 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCGGCGGTGGGAGTGAA+TGG | 0.339972 | tig0019315:-28439 | Msa1372770:intergenic |
| TAGGATTAATTCAACTTCAA+TGG | 0.349493 | tig0019315:+28348 | Msa1372770:CDS |
| ACGAGTTGTCGGAAATATAA+TGG | 0.371922 | tig0019315:+28309 | Msa1372770:CDS |
| AACGATGAGAACAAAGTCTT+TGG | 0.378667 | tig0019315:+28840 | Msa1372770:CDS |
| GCTTCCCTCGTTTCTTCTCC+AGG | 0.415645 | tig0019315:+28586 | Msa1372770:CDS |
| TTGCGGCGGTGGGAGTGAAT+GGG | 0.416301 | tig0019315:-28438 | Msa1372770:intergenic |
| AGGAGAGAACCTTGCGGCGG+TGG | 0.417046 | tig0019315:-28449 | Msa1372770:intergenic |
| AGGAATACCAGTGGAATCAT+TGG | 0.427164 | tig0019315:-28899 | Msa1372770:intergenic |
| TGGAGAGGCTGCAGCGGCGG+CGG | 0.429831 | tig0019315:-28483 | Msa1372770:intergenic |
| GATGAAGTTGCGCGTGAACT+AGG | 0.440088 | tig0019315:+28717 | Msa1372770:CDS |
| TGGAGATAGGAGGAAGTTGT+AGG | 0.459668 | tig0019315:+28329 | Msa1372770:CDS |
| GGGAAGCTCTGGTGGAGAAA+TGG | 0.467047 | tig0019315:-28570 | Msa1372770:intergenic |
| AAGCAATCTTGCGGTGGAAG+TGG | 0.470711 | tig0019315:-28503 | Msa1372770:intergenic |
| TGGAATCATTGGGAGGAGTT+CGG | 0.481246 | tig0019315:-28888 | Msa1372770:intergenic |
| CCTTTGAGTAACTCAACAAA+TGG | 0.487250 | tig0019315:-28976 | Msa1372770:intergenic |
| AGAGGCTGCAGCGGCGGCGG+AGG | 0.490190 | tig0019315:-28480 | Msa1372770:intergenic |
| CGGCGGCGGAGGAGAGTCGG+AGG | 0.497341 | tig0019315:-28469 | Msa1372770:intergenic |
| AGAAGAAACGAGGGAAGCTC+TGG | 0.499568 | tig0019315:-28581 | Msa1372770:intergenic |
| GTCGGAAATATAATGGAGAT+AGG | 0.500076 | tig0019315:+28316 | Msa1372770:CDS |
| ATGAAGTTGCGCGTGAACTA+GGG | 0.520837 | tig0019315:+28718 | Msa1372770:CDS |
| GGAATACCAGTGGAATCATT+GGG | 0.526341 | tig0019315:-28898 | Msa1372770:intergenic |
| AGTGAATGGGGATAGAACGG+AGG | 0.527100 | tig0019315:-28425 | Msa1372770:intergenic |
| CAGCGGCGGCGGAGGAGAGT+CGG | 0.531320 | tig0019315:-28472 | Msa1372770:intergenic |
| CATTCACTCCCACCGCCGCA+AGG | 0.531835 | tig0019315:+28440 | Msa1372770:CDS |
| AGGCAGTATAAACGAGTTGT+CGG | 0.533746 | tig0019315:+28298 | Msa1372770:CDS |
| AGAAACGAGGGAAGCTCTGG+TGG | 0.546722 | tig0019315:-28578 | Msa1372770:intergenic |
| AAGTGGAGAGGCTGCAGCGG+CGG | 0.548881 | tig0019315:-28486 | Msa1372770:intergenic |
| GGAGAGAACCTTGCGGCGGT+GGG | 0.557924 | tig0019315:-28448 | Msa1372770:intergenic |
| GGGAGTGAATGGGGATAGAA+CGG | 0.561215 | tig0019315:-28428 | Msa1372770:intergenic |
| TCAACAAATGGTTCTTTGAG+AGG | 0.563123 | tig0019315:-28964 | Msa1372770:intergenic |
| GTGAATGGGGATAGAACGGA+GGG | 0.568796 | tig0019315:-28424 | Msa1372770:intergenic |
| TGCGGCGGTGGGAGTGAATG+GGG | 0.574016 | tig0019315:-28437 | Msa1372770:intergenic |
| TGGAAGTGGAGAGGCTGCAG+CGG | 0.577141 | tig0019315:-28489 | Msa1372770:intergenic |
| CTCTGGTGGAGAAATGGTTG+CGG | 0.585366 | tig0019315:-28564 | Msa1372770:intergenic |
| TTCAACTTCAATGGAAAGAG+CGG | 0.586601 | tig0019315:+28357 | Msa1372770:CDS |
| CCATTTGTTGAGTTACTCAA+AGG | 0.586706 | tig0019315:+28960 | Msa1372770:CDS |
| ACTCCTCCCAATGATTCCAC+TGG | 0.595991 | tig0019315:+28876 | Msa1372770:CDS |
| TGGTGGTGGCGAAGTAGTGA+AGG | 0.601630 | tig0019315:+28695 | Msa1372770:CDS |
| ATCTTGCGGTGGAAGTGGAG+AGG | 0.618421 | tig0019315:-28498 | Msa1372770:intergenic |
| GGAAATATAATGGAGATAGG+AGG | 0.619551 | tig0019315:+28319 | Msa1372770:CDS |
| GAATGGGGATAGAACGGAGG+GGG | 0.623314 | tig0019315:-28422 | Msa1372770:intergenic |
| TGAATGGGGATAGAACGGAG+GGG | 0.623959 | tig0019315:-28423 | Msa1372770:intergenic |
| AGAAGAAAGCAATCTTGCGG+TGG | 0.653139 | tig0019315:-28509 | Msa1372770:intergenic |
| ACACTGTGCTCTAAAATGTG+AGG | 0.655510 | tig0019315:-28919 | Msa1372770:intergenic |
| CATACCTGGAGAAGAAACGA+GGG | 0.675730 | tig0019315:-28590 | Msa1372770:intergenic |
| TAAAATGTGAGGAATACCAG+TGG | 0.680348 | tig0019315:-28908 | Msa1372770:intergenic |
| GGGAGATGTATACACGATGG+AGG | 0.682546 | tig0019315:+28278 | Msa1372770:exon |
| ACATACCTGGAGAAGAAACG+AGG | 0.687833 | tig0019315:-28591 | None:intergenic |
| CGGAGGAGAGAACCTTGCGG+CGG | 0.695662 | tig0019315:-28452 | None:intergenic |
| AGAAGAAGAAAGCAATCTTG+CGG | 0.695847 | tig0019315:-28512 | None:intergenic |
| ATACCAGTGGAATCATTGGG+AGG | 0.698254 | tig0019315:-28895 | None:intergenic |
| TAGGGGAGATGTATACACGA+TGG | 0.703466 | tig0019315:+28275 | None:intergenic |
| CGGTTACGAAAGTAAAAGAG+CGG | 0.719946 | tig0019315:-28544 | Msa1372760:intergenic |
| GGTGCGAGAGAAATATCTGG+TGG | 0.721010 | tig0019315:-28401 | Msa1372760:intergenic |
| AGTCGGAGGAGAGAACCTTG+CGG | 0.746794 | tig0019315:-28455 | Msa1372760:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATTATTATTATTATTA+TGG | - | tig0019315:28652-28671 | Msa1372770:intergenic | 0.0% |
| !!! | AATAATAATGATGATGATTT+TGG | + | tig0019315:28665-28684 | Msa1372770:intron | 15.0% |
| ! | TAGGATTAATTCAACTTCAA+TGG | + | tig0019315:28348-28367 | Msa1372770:CDS | 25.0% |
| !!! | ATGATGATTTTGGTAGATAT+TGG | + | tig0019315:28675-28694 | Msa1372770:intron | 25.0% |
| AATAGATAGAGATACATACC+TGG | - | tig0019315:28607-28626 | Msa1372770:intergenic | 30.0% | |
| !! | ATGATTTTGGTAGATATTGG+TGG | + | tig0019315:28678-28697 | Msa1372770:intron | 30.0% |
| ACGAGTTGTCGGAAATATAA+TGG | + | tig0019315:28309-28328 | Msa1372770:CDS | 35.0% | |
| ! | GTCGGAAATATAATGGAGAT+AGG | + | tig0019315:28316-28335 | Msa1372770:CDS | 35.0% |
| ! | GGAAATATAATGGAGATAGG+AGG | + | tig0019315:28319-28338 | Msa1372770:CDS | 35.0% |
| TTCAACTTCAATGGAAAGAG+CGG | + | tig0019315:28357-28376 | Msa1372770:CDS | 35.0% | |
| AGAAGAAGAAAGCAATCTTG+CGG | - | tig0019315:28515-28534 | Msa1372770:intergenic | 35.0% | |
| !! | ATTTTGGTAGATATTGGTGG+TGG | + | tig0019315:28681-28700 | Msa1372770:intron | 35.0% |
| !!! | ACTGCTTTTGATTTGCATTC+TGG | - | tig0019315:28771-28790 | Msa1372770:intergenic | 35.0% |
| AACGATGAGAACAAAGTCTT+TGG | + | tig0019315:28840-28859 | Msa1372770:CDS | 35.0% | |
| TAAAATGTGAGGAATACCAG+TGG | - | tig0019315:28895-28914 | Msa1372770:intergenic | 35.0% | |
| TCAACAAATGGTTCTTTGAG+AGG | - | tig0019315:28951-28970 | Msa1372770:intergenic | 35.0% | |
| CCTTTGAGTAACTCAACAAA+TGG | - | tig0019315:28963-28982 | Msa1372770:intergenic | 35.0% | |
| ! | CCATTTGTTGAGTTACTCAA+AGG | + | tig0019315:28960-28979 | Msa1372770:CDS | 35.0% |
| AGGCAGTATAAACGAGTTGT+CGG | + | tig0019315:28298-28317 | Msa1372770:CDS | 40.0% | |
| CGGTTACGAAAGTAAAAGAG+CGG | - | tig0019315:28547-28566 | Msa1372770:intergenic | 40.0% | |
| ! | CTTTTCAAACACGTCAAGAC+AGG | + | tig0019315:28792-28811 | Msa1372770:CDS | 40.0% |
| GGAATACCAGTGGAATCATT+GGG | - | tig0019315:28885-28904 | Msa1372770:intergenic | 40.0% | |
| AGGAATACCAGTGGAATCAT+TGG | - | tig0019315:28886-28905 | Msa1372770:intergenic | 40.0% | |
| !! | ACACTGTGCTCTAAAATGTG+AGG | - | tig0019315:28906-28925 | Msa1372770:intergenic | 40.0% |
| ! | TTAGAGCACAGTGTTTTGTG+TGG | + | tig0019315:28912-28931 | Msa1372770:CDS | 40.0% |
| TGGAGATAGGAGGAAGTTGT+AGG | + | tig0019315:28329-28348 | Msa1372770:CDS | 45.0% | |
| AGAAGAAAGCAATCTTGCGG+TGG | - | tig0019315:28512-28531 | Msa1372770:intergenic | 45.0% | |
| CATACCTGGAGAAGAAACGA+GGG | - | tig0019315:28593-28612 | Msa1372770:intergenic | 45.0% | |
| ACATACCTGGAGAAGAAACG+AGG | - | tig0019315:28594-28613 | Msa1372770:intergenic | 45.0% | |
| ATGAAGTTGCGCGTGAACTA+GGG | + | tig0019315:28718-28737 | Msa1372770:CDS | 45.0% | |
| TGGAATCATTGGGAGGAGTT+CGG | - | tig0019315:28875-28894 | Msa1372770:intergenic | 45.0% | |
| ATACCAGTGGAATCATTGGG+AGG | - | tig0019315:28882-28901 | Msa1372770:intergenic | 45.0% | |
| GGGAGATGTATACACGATGG+AGG | + | tig0019315:28278-28297 | Msa1372770:exon | 50.0% | |
| GGTGCGAGAGAAATATCTGG+TGG | - | tig0019315:28404-28423 | Msa1372770:intergenic | 50.0% | |
| TGAATGGGGATAGAACGGAG+GGG | - | tig0019315:28426-28445 | Msa1372770:intergenic | 50.0% | |
| GTGAATGGGGATAGAACGGA+GGG | - | tig0019315:28427-28446 | Msa1372770:intergenic | 50.0% | |
| AGTGAATGGGGATAGAACGG+AGG | - | tig0019315:28428-28447 | Msa1372770:intergenic | 50.0% | |
| GGGAGTGAATGGGGATAGAA+CGG | - | tig0019315:28431-28450 | Msa1372770:intergenic | 50.0% | |
| AAGCAATCTTGCGGTGGAAG+TGG | - | tig0019315:28506-28525 | Msa1372770:intergenic | 50.0% | |
| CTCTGGTGGAGAAATGGTTG+CGG | - | tig0019315:28567-28586 | Msa1372770:intergenic | 50.0% | |
| AGAAGAAACGAGGGAAGCTC+TGG | - | tig0019315:28584-28603 | Msa1372770:intergenic | 50.0% | |
| GATGAAGTTGCGCGTGAACT+AGG | + | tig0019315:28717-28736 | Msa1372770:CDS | 50.0% | |
| ACTCCTCCCAATGATTCCAC+TGG | + | tig0019315:28876-28895 | Msa1372770:CDS | 50.0% | |
| ! | GGGGGTGCGAGAGAAATATC+TGG | - | tig0019315:28407-28426 | Msa1372770:intergenic | 55.0% |
| GAATGGGGATAGAACGGAGG+GGG | - | tig0019315:28425-28444 | Msa1372770:intergenic | 55.0% | |
| AGTCGGAGGAGAGAACCTTG+CGG | - | tig0019315:28458-28477 | Msa1372770:intergenic | 55.0% | |
| ATCTTGCGGTGGAAGTGGAG+AGG | - | tig0019315:28501-28520 | Msa1372770:intergenic | 55.0% | |
| ! | GGGAAGCTCTGGTGGAGAAA+TGG | - | tig0019315:28573-28592 | Msa1372770:intergenic | 55.0% |
| ! | AGAAACGAGGGAAGCTCTGG+TGG | - | tig0019315:28581-28600 | Msa1372770:intergenic | 55.0% |
| GCTTCCCTCGTTTCTTCTCC+AGG | + | tig0019315:28586-28605 | Msa1372770:CDS | 55.0% | |
| !! | TGGTGGTGGCGAAGTAGTGA+AGG | + | tig0019315:28695-28714 | Msa1372770:CDS | 55.0% |
| TTGCGGCGGTGGGAGTGAAT+GGG | - | tig0019315:28441-28460 | Msa1372770:intergenic | 60.0% | |
| TGGAAGTGGAGAGGCTGCAG+CGG | - | tig0019315:28492-28511 | Msa1372770:intergenic | 60.0% | |
| TGCGGCGGTGGGAGTGAATG+GGG | - | tig0019315:28440-28459 | Msa1372770:intergenic | 65.0% | |
| CTTGCGGCGGTGGGAGTGAA+TGG | - | tig0019315:28442-28461 | Msa1372770:intergenic | 65.0% | |
| CATTCACTCCCACCGCCGCA+AGG | + | tig0019315:28440-28459 | Msa1372770:CDS | 65.0% | |
| GGAGAGAACCTTGCGGCGGT+GGG | - | tig0019315:28451-28470 | Msa1372770:intergenic | 65.0% | |
| AGGAGAGAACCTTGCGGCGG+TGG | - | tig0019315:28452-28471 | Msa1372770:intergenic | 65.0% | |
| CGGAGGAGAGAACCTTGCGG+CGG | - | tig0019315:28455-28474 | Msa1372770:intergenic | 65.0% | |
| AAGTGGAGAGGCTGCAGCGG+CGG | - | tig0019315:28489-28508 | Msa1372770:intergenic | 65.0% | |
| CAGCGGCGGCGGAGGAGAGT+CGG | - | tig0019315:28475-28494 | Msa1372770:intergenic | 75.0% | |
| TGGAGAGGCTGCAGCGGCGG+CGG | - | tig0019315:28486-28505 | Msa1372770:intergenic | 75.0% | |
| ! | CGGCGGCGGAGGAGAGTCGG+AGG | - | tig0019315:28472-28491 | Msa1372770:intergenic | 80.0% |
| ! | AGAGGCTGCAGCGGCGGCGG+AGG | - | tig0019315:28483-28502 | Msa1372770:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0019315 | gene | 28278 | 29001 | 28278 | ID=Msa1372770;Name=Msa1372770 |
| tig0019315 | mRNA | 28278 | 29001 | 28278 | ID=Msa1372770-mRNA-1;Parent=Msa1372770;Name=Msa1372770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=5|1|0.5|1|1|1|2|0|212 |
| tig0019315 | exon | 28278 | 28607 | 28278 | ID=Msa1372770-mRNA-1:exon:2910;Parent=Msa1372770-mRNA-1 |
| tig0019315 | exon | 28691 | 29001 | 28691 | ID=Msa1372770-mRNA-1:exon:2911;Parent=Msa1372770-mRNA-1 |
| tig0019315 | five_prime_UTR | 28278 | 28282 | 28278 | ID=Msa1372770-mRNA-1:five_prime_utr;Parent=Msa1372770-mRNA-1 |
| tig0019315 | CDS | 28283 | 28607 | 28283 | ID=Msa1372770-mRNA-1:cds;Parent=Msa1372770-mRNA-1 |
| tig0019315 | CDS | 28691 | 29001 | 28691 | ID=Msa1372770-mRNA-1:cds;Parent=Msa1372770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
