AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0155210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0155210 MtrunA17_Chr1g0165831 92.208 231 18 0 1 231 1 231 3.32e-152 423
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0155210 AT5G43650.1 36.486 222 128 6 14 231 16 228 8.99e-35 125
Msa0155210 AT5G43650.2 35.407 209 122 6 14 218 16 215 2.47e-30 112

Find 37 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATCACCTAGAAGACTTTC+AGG 0.237157 1_4:-20420252 None:intergenic
CTTGTTCATGTTATTTGAAT+TGG 0.244140 1_4:-20420418 None:intergenic
GACCAACCCAAGGTTAGTTT+TGG 0.266186 1_4:+20420372 Msa0155210:CDS
TGAACAAAAGCACTTAGATT+TGG 0.329794 1_4:-20420342 None:intergenic
CTCAAATTGTTGAAAGACTA+TGG 0.337935 1_4:+20421021 Msa0155210:CDS
ACAAGGATGCAAAGAGGAAT+TGG 0.356536 1_4:+20420885 Msa0155210:CDS
GAGAGCACTTGCACTGTGTT+TGG 0.396041 1_4:+20421301 Msa0155210:CDS
AGAGTCCCATGTCCTACATC+TGG 0.437447 1_4:+20420976 Msa0155210:CDS
TTGGTTTGGTGAAAGATTGC+AGG 0.448525 1_4:+20421254 Msa0155210:intron
TGTTCCCCAAAACTAACCTT+GGG 0.455941 1_4:-20420378 None:intergenic
CTTTGGTTTGTAGCTAAAAG+AGG 0.465381 1_4:-20420288 None:intergenic
GGGGAACAAAGTTCTCGAAA+AGG 0.474729 1_4:+20420393 Msa0155210:CDS
TGCAGGAACTGATGTGGAAA+AGG 0.474929 1_4:+20421271 Msa0155210:CDS
GGGAACAAAGTTCTCGAAAA+GGG 0.478191 1_4:+20420394 Msa0155210:CDS
TTGTTCCCCAAAACTAACCT+TGG 0.481166 1_4:-20420379 None:intergenic
GTGTTTGAACAAAACAACTT+TGG 0.492561 1_4:-20420305 None:intergenic
TGTAGTACAAACAGCAGCTA+AGG 0.514214 1_4:+20420849 Msa0155210:CDS
CTTAGATTTGGTGTAGGACA+AGG 0.518149 1_4:-20420330 None:intergenic
GTAGAATCAATTCCAGATGT+AGG 0.538154 1_4:-20420988 None:intergenic
ACTACTTTATTTCTCTCTAG+AGG 0.542226 1_4:-20420471 None:intergenic
TTACATGCAGTGCTACCTCA+TGG 0.551125 1_4:+20420576 Msa0155210:CDS
AAGGAAATTCAAAGGCTACA+AGG 0.552769 1_4:+20420868 Msa0155210:CDS
CCCCAAAACTAACCTTGGGT+TGG 0.563725 1_4:-20420374 None:intergenic
ATTCAAATAACATGAACAAG+AGG 0.578747 1_4:+20420421 Msa0155210:CDS
AGTCTTGGAGATAGAATCTG+AGG 0.586101 1_4:+20421095 Msa0155210:CDS
ACATGATTAGTGAAAGAATG+AGG 0.598991 1_4:+20420523 Msa0155210:CDS
TCAATTCCAGATGTAGGACA+TGG 0.602329 1_4:-20420982 None:intergenic
AAAGCACTTAGATTTGGTGT+AGG 0.616910 1_4:-20420336 None:intergenic
TCTAGAGAGAAATAAAGTAG+TGG 0.617344 1_4:+20420473 Msa0155210:CDS
CAGCAGCTAAGGAAATTCAA+AGG 0.623684 1_4:+20420860 Msa0155210:CDS
GATGCAAAGAGGAATTGGAA+AGG 0.633307 1_4:+20420890 Msa0155210:CDS
CAATTCCAGATGTAGGACAT+GGG 0.675336 1_4:-20420981 None:intergenic
GTGGAATGTGAAAGAGAACG+TGG 0.683740 1_4:+20420492 Msa0155210:CDS
ATATATACTTTGGTTCCATG+AGG 0.684445 1_4:-20420591 None:intergenic
AAAGATTGCAGGAACTGATG+TGG 0.688766 1_4:+20421265 Msa0155210:intron
AAGGCTACAAGGATGCAAAG+AGG 0.691456 1_4:+20420879 Msa0155210:CDS
TCAATATAGAGACCAACCCA+AGG 0.710841 1_4:+20420362 Msa0155210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATATATTTTTTAAAAT+AGG - chr1_4:20421133-20421152 None:intergenic 0.0%
!!! AAATATATATTTTTTAAAAT+AGG - chr1_4:20421133-20421152 None:intergenic 0.0%
!! TCATAAATATATGTTAAATA+TGG - chr1_4:20420724-20420743 None:intergenic 10.0%
!!! TTTTTTTCTTTTTTCTTTTA+TGG + chr1_4:20420809-20420828 Msa0155210:intron 10.0%
!!! TAATTTTTTAAAACTATACA+AGG + chr1_4:20421156-20421175 Msa0155210:intron 10.0%
!! TCATAAATATATGTTAAATA+TGG - chr1_4:20420724-20420743 None:intergenic 10.0%
!!! TTTTTTTCTTTTTTCTTTTA+TGG + chr1_4:20420809-20420828 Msa0155210:intron 10.0%
!!! TAATTTTTTAAAACTATACA+AGG + chr1_4:20421156-20421175 Msa0155210:intron 10.0%
!!! GAATGTTTTTATATATACTT+TGG - chr1_4:20420604-20420623 None:intergenic 15.0%
!!! AAAAAAGTGCTTATTTTAAA+AGG - chr1_4:20420748-20420767 None:intergenic 15.0%
!!! GAATGTTTTTATATATACTT+TGG - chr1_4:20420604-20420623 None:intergenic 15.0%
!!! AAAAAAGTGCTTATTTTAAA+AGG - chr1_4:20420748-20420767 None:intergenic 15.0%
!! TAATTGTGTTTGTAACATAA+AGG - chr1_4:20420678-20420697 None:intergenic 20.0%
!! TTATGTTACAAACACAATTA+TGG + chr1_4:20420678-20420697 Msa0155210:intron 20.0%
!! TAATTGTGTTTGTAACATAA+AGG - chr1_4:20420678-20420697 None:intergenic 20.0%
!! TTATGTTACAAACACAATTA+TGG + chr1_4:20420678-20420697 Msa0155210:intron 20.0%
!! CTTGTTCATGTTATTTGAAT+TGG - chr1_4:20420421-20420440 None:intergenic 25.0%
! ATTCAAATAACATGAACAAG+AGG + chr1_4:20420421-20420440 Msa0155210:CDS 25.0%
!!! TCATTTCAAGCTATTTCTAA+TGG + chr1_4:20420773-20420792 Msa0155210:intron 25.0%
! TGTTTATGCTAAAAATGTGT+TGG + chr1_4:20421235-20421254 Msa0155210:intron 25.0%
!! CTTGTTCATGTTATTTGAAT+TGG - chr1_4:20420421-20420440 None:intergenic 25.0%
! ATTCAAATAACATGAACAAG+AGG + chr1_4:20420421-20420440 Msa0155210:CDS 25.0%
!!! TCATTTCAAGCTATTTCTAA+TGG + chr1_4:20420773-20420792 Msa0155210:intron 25.0%
! TGTTTATGCTAAAAATGTGT+TGG + chr1_4:20421235-20421254 Msa0155210:intron 25.0%
!!! TTTTTTCCTGAAAGTCTTCT+AGG + chr1_4:20420246-20420265 Msa0155210:CDS 30.0%
!!! TCTTCTAGGTGATGTTTTTT+GGG + chr1_4:20420260-20420279 Msa0155210:CDS 30.0%
GTGTTTGAACAAAACAACTT+TGG - chr1_4:20420308-20420327 None:intergenic 30.0%
!! TGAACAAAAGCACTTAGATT+TGG - chr1_4:20420345-20420364 None:intergenic 30.0%
! ACTACTTTATTTCTCTCTAG+AGG - chr1_4:20420474-20420493 None:intergenic 30.0%
TCTAGAGAGAAATAAAGTAG+TGG + chr1_4:20420473-20420492 Msa0155210:CDS 30.0%
ACATGATTAGTGAAAGAATG+AGG + chr1_4:20420523-20420542 Msa0155210:CDS 30.0%
! ATATATACTTTGGTTCCATG+AGG - chr1_4:20420594-20420613 None:intergenic 30.0%
CACATCGTATATAGAAATTG+CGG + chr1_4:20420623-20420642 Msa0155210:intron 30.0%
!! TTTCAAGCTATTTCTAATGG+TGG + chr1_4:20420776-20420795 Msa0155210:intron 30.0%
CTCAAATTGTTGAAAGACTA+TGG + chr1_4:20421021-20421040 Msa0155210:CDS 30.0%
!! ATGCTAAAAATGTGTTGGTT+TGG + chr1_4:20421240-20421259 Msa0155210:intron 30.0%
!!! TTTTTTCCTGAAAGTCTTCT+AGG + chr1_4:20420246-20420265 Msa0155210:CDS 30.0%
!!! TCTTCTAGGTGATGTTTTTT+GGG + chr1_4:20420260-20420279 Msa0155210:CDS 30.0%
GTGTTTGAACAAAACAACTT+TGG - chr1_4:20420308-20420327 None:intergenic 30.0%
!! TGAACAAAAGCACTTAGATT+TGG - chr1_4:20420345-20420364 None:intergenic 30.0%
! ACTACTTTATTTCTCTCTAG+AGG - chr1_4:20420474-20420493 None:intergenic 30.0%
TCTAGAGAGAAATAAAGTAG+TGG + chr1_4:20420473-20420492 Msa0155210:CDS 30.0%
ACATGATTAGTGAAAGAATG+AGG + chr1_4:20420523-20420542 Msa0155210:CDS 30.0%
! ATATATACTTTGGTTCCATG+AGG - chr1_4:20420594-20420613 None:intergenic 30.0%
CACATCGTATATAGAAATTG+CGG + chr1_4:20420623-20420642 Msa0155210:intron 30.0%
!! TTTCAAGCTATTTCTAATGG+TGG + chr1_4:20420776-20420795 Msa0155210:intron 30.0%
CTCAAATTGTTGAAAGACTA+TGG + chr1_4:20421021-20421040 Msa0155210:CDS 30.0%
!! ATGCTAAAAATGTGTTGGTT+TGG + chr1_4:20421240-20421259 Msa0155210:intron 30.0%
!!! GTCTTCTAGGTGATGTTTTT+TGG + chr1_4:20420259-20420278 Msa0155210:CDS 35.0%
!! CTTTGGTTTGTAGCTAAAAG+AGG - chr1_4:20420291-20420310 None:intergenic 35.0%
!! AAAGCACTTAGATTTGGTGT+AGG - chr1_4:20420339-20420358 None:intergenic 35.0%
! AGAGGATGATTGCATTTTTG+AGG + chr1_4:20420439-20420458 Msa0155210:CDS 35.0%
AAGGAAATTCAAAGGCTACA+AGG + chr1_4:20420868-20420887 Msa0155210:CDS 35.0%
GTAGAATCAATTCCAGATGT+AGG - chr1_4:20420991-20421010 None:intergenic 35.0%
! CAAGTCAAATTTTTCTCAGC+AGG + chr1_4:20421062-20421081 Msa0155210:CDS 35.0%
!!! GTCTTCTAGGTGATGTTTTT+TGG + chr1_4:20420259-20420278 Msa0155210:CDS 35.0%
!! CTTTGGTTTGTAGCTAAAAG+AGG - chr1_4:20420291-20420310 None:intergenic 35.0%
!! AAAGCACTTAGATTTGGTGT+AGG - chr1_4:20420339-20420358 None:intergenic 35.0%
! AGAGGATGATTGCATTTTTG+AGG + chr1_4:20420439-20420458 Msa0155210:CDS 35.0%
AAGGAAATTCAAAGGCTACA+AGG + chr1_4:20420868-20420887 Msa0155210:CDS 35.0%
GTAGAATCAATTCCAGATGT+AGG - chr1_4:20420991-20421010 None:intergenic 35.0%
! CAAGTCAAATTTTTCTCAGC+AGG + chr1_4:20421062-20421081 Msa0155210:CDS 35.0%
ACATCACCTAGAAGACTTTC+AGG - chr1_4:20420255-20420274 None:intergenic 40.0%
!! CTTAGATTTGGTGTAGGACA+AGG - chr1_4:20420333-20420352 None:intergenic 40.0%
TCAATATAGAGACCAACCCA+AGG + chr1_4:20420362-20420381 Msa0155210:CDS 40.0%
!!! ACCAACCCAAGGTTAGTTTT+GGG + chr1_4:20420373-20420392 Msa0155210:CDS 40.0%
TGTTCCCCAAAACTAACCTT+GGG - chr1_4:20420381-20420400 None:intergenic 40.0%
TTGTTCCCCAAAACTAACCT+TGG - chr1_4:20420382-20420401 None:intergenic 40.0%
GGGAACAAAGTTCTCGAAAA+GGG + chr1_4:20420394-20420413 Msa0155210:CDS 40.0%
ACAAACACAATTATGGTCGC+AGG + chr1_4:20420685-20420704 Msa0155210:intron 40.0%
TGTAGTACAAACAGCAGCTA+AGG + chr1_4:20420849-20420868 Msa0155210:CDS 40.0%
CAGCAGCTAAGGAAATTCAA+AGG + chr1_4:20420860-20420879 Msa0155210:CDS 40.0%
ACAAGGATGCAAAGAGGAAT+TGG + chr1_4:20420885-20420904 Msa0155210:CDS 40.0%
GATGCAAAGAGGAATTGGAA+AGG + chr1_4:20420890-20420909 Msa0155210:CDS 40.0%
CAATTCCAGATGTAGGACAT+GGG - chr1_4:20420984-20421003 None:intergenic 40.0%
TCAATTCCAGATGTAGGACA+TGG - chr1_4:20420985-20421004 None:intergenic 40.0%
!!! GCAGGAGTTGTTTTTAGTCT+TGG + chr1_4:20421080-20421099 Msa0155210:CDS 40.0%
AGTCTTGGAGATAGAATCTG+AGG + chr1_4:20421095-20421114 Msa0155210:CDS 40.0%
!! TTGGTTTGGTGAAAGATTGC+AGG + chr1_4:20421254-20421273 Msa0155210:intron 40.0%
AAAGATTGCAGGAACTGATG+TGG + chr1_4:20421265-20421284 Msa0155210:intron 40.0%
ACATCACCTAGAAGACTTTC+AGG - chr1_4:20420255-20420274 None:intergenic 40.0%
!! CTTAGATTTGGTGTAGGACA+AGG - chr1_4:20420333-20420352 None:intergenic 40.0%
TCAATATAGAGACCAACCCA+AGG + chr1_4:20420362-20420381 Msa0155210:CDS 40.0%
!!! ACCAACCCAAGGTTAGTTTT+GGG + chr1_4:20420373-20420392 Msa0155210:CDS 40.0%
TGTTCCCCAAAACTAACCTT+GGG - chr1_4:20420381-20420400 None:intergenic 40.0%
TTGTTCCCCAAAACTAACCT+TGG - chr1_4:20420382-20420401 None:intergenic 40.0%
GGGAACAAAGTTCTCGAAAA+GGG + chr1_4:20420394-20420413 Msa0155210:CDS 40.0%
ACAAACACAATTATGGTCGC+AGG + chr1_4:20420685-20420704 Msa0155210:intron 40.0%
TGTAGTACAAACAGCAGCTA+AGG + chr1_4:20420849-20420868 Msa0155210:CDS 40.0%
CAGCAGCTAAGGAAATTCAA+AGG + chr1_4:20420860-20420879 Msa0155210:CDS 40.0%
ACAAGGATGCAAAGAGGAAT+TGG + chr1_4:20420885-20420904 Msa0155210:CDS 40.0%
GATGCAAAGAGGAATTGGAA+AGG + chr1_4:20420890-20420909 Msa0155210:CDS 40.0%
CAATTCCAGATGTAGGACAT+GGG - chr1_4:20420984-20421003 None:intergenic 40.0%
TCAATTCCAGATGTAGGACA+TGG - chr1_4:20420985-20421004 None:intergenic 40.0%
!!! GCAGGAGTTGTTTTTAGTCT+TGG + chr1_4:20421080-20421099 Msa0155210:CDS 40.0%
AGTCTTGGAGATAGAATCTG+AGG + chr1_4:20421095-20421114 Msa0155210:CDS 40.0%
!! TTGGTTTGGTGAAAGATTGC+AGG + chr1_4:20421254-20421273 Msa0155210:intron 40.0%
AAAGATTGCAGGAACTGATG+TGG + chr1_4:20421265-20421284 Msa0155210:intron 40.0%
!!! GACCAACCCAAGGTTAGTTT+TGG + chr1_4:20420372-20420391 Msa0155210:CDS 45.0%
!!! CCAACCCAAGGTTAGTTTTG+GGG + chr1_4:20420374-20420393 Msa0155210:CDS 45.0%
GGGGAACAAAGTTCTCGAAA+AGG + chr1_4:20420393-20420412 Msa0155210:CDS 45.0%
GTGGAATGTGAAAGAGAACG+TGG + chr1_4:20420492-20420511 Msa0155210:CDS 45.0%
TTACATGCAGTGCTACCTCA+TGG + chr1_4:20420576-20420595 Msa0155210:CDS 45.0%
AAGGCTACAAGGATGCAAAG+AGG + chr1_4:20420879-20420898 Msa0155210:CDS 45.0%
TGCAGGAACTGATGTGGAAA+AGG + chr1_4:20421271-20421290 Msa0155210:CDS 45.0%
!!! GACCAACCCAAGGTTAGTTT+TGG + chr1_4:20420372-20420391 Msa0155210:CDS 45.0%
!!! CCAACCCAAGGTTAGTTTTG+GGG + chr1_4:20420374-20420393 Msa0155210:CDS 45.0%
GGGGAACAAAGTTCTCGAAA+AGG + chr1_4:20420393-20420412 Msa0155210:CDS 45.0%
GTGGAATGTGAAAGAGAACG+TGG + chr1_4:20420492-20420511 Msa0155210:CDS 45.0%
TTACATGCAGTGCTACCTCA+TGG + chr1_4:20420576-20420595 Msa0155210:CDS 45.0%
AAGGCTACAAGGATGCAAAG+AGG + chr1_4:20420879-20420898 Msa0155210:CDS 45.0%
TGCAGGAACTGATGTGGAAA+AGG + chr1_4:20421271-20421290 Msa0155210:CDS 45.0%
CCCCAAAACTAACCTTGGGT+TGG - chr1_4:20420377-20420396 None:intergenic 50.0%
AGAGTCCCATGTCCTACATC+TGG + chr1_4:20420976-20420995 Msa0155210:CDS 50.0%
!! GAGAGCACTTGCACTGTGTT+TGG + chr1_4:20421301-20421320 Msa0155210:CDS 50.0%
CCCCAAAACTAACCTTGGGT+TGG - chr1_4:20420377-20420396 None:intergenic 50.0%
AGAGTCCCATGTCCTACATC+TGG + chr1_4:20420976-20420995 Msa0155210:CDS 50.0%
!! GAGAGCACTTGCACTGTGTT+TGG + chr1_4:20421301-20421320 Msa0155210:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 20420237 20421337 20420237 ID=Msa0155210;Name=Msa0155210
chr1_4 mRNA 20420237 20421337 20420237 ID=Msa0155210-mRNA-1;Parent=Msa0155210;Name=Msa0155210-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|3|0|239
chr1_4 exon 20420237 20420605 20420237 ID=Msa0155210-mRNA-1:exon:7426;Parent=Msa0155210-mRNA-1
chr1_4 exon 20420835 20421116 20420835 ID=Msa0155210-mRNA-1:exon:7427;Parent=Msa0155210-mRNA-1
chr1_4 exon 20421269 20421337 20421269 ID=Msa0155210-mRNA-1:exon:7428;Parent=Msa0155210-mRNA-1
chr1_4 CDS 20420237 20420605 20420237 ID=Msa0155210-mRNA-1:cds;Parent=Msa0155210-mRNA-1
chr1_4 CDS 20420835 20421116 20420835 ID=Msa0155210-mRNA-1:cds;Parent=Msa0155210-mRNA-1
chr1_4 CDS 20421269 20421337 20421269 ID=Msa0155210-mRNA-1:cds;Parent=Msa0155210-mRNA-1
Gene Sequence

>Msa0155210

ATGGAACAGTTTTTTCCTGAAAGTCTTCTAGGTGATGTTTTTTGGGATGAACCTCTTTTAGCTACAAACCAAAGTTGTTTTGTTCAAACACAACCTTGTCCTACACCAAATCTAAGTGCTTTTGTTCAATATAGAGACCAACCCAAGGTTAGTTTTGGGGAACAAAGTTCTCGAAAAGGGTCCAATTCAAATAACATGAACAAGAGGATGATTGCATTTTTGAGGAAAAGTTTGCCTCTAGAGAGAAATAAAGTAGTGGAATGTGAAAGAGAACGTGGTTTCAAGCACATGATTAGTGAAAGAATGAGGAGACAAAGACAAAGACAATGTTGCTTCAACTTACATGCAGTGCTACCTCATGGAACCAAAACAGATAATAATTCTGTAGTACAAACAGCAGCTAAGGAAATTCAAAGGCTACAAGGATGCAAAGAGGAATTGGAAAGGAAAAACTTTGTGCTTGAAAAAAATGTTGAAGTAATTGATAGAAGAAAACAAATTCAATATTTGAGAGTCCCATGTCCTACATCTGGAATTGATTCTACAGTTGAGACACTCAAATTGTTGAAAGACTATGGTATTGATACTAAAAGCGTCAAGTCAAATTTTTCTCAGCAGGAGTTGTTTTTAGTCTTGGAGATAGAATCTGAGATTGCAGGAACTGATGTGGAAAAGGCTATTCAGAGAGCACTTGCACTGTGTTTGGTTGAAGAGAAATAG

Protein sequence

>Msa0155210

MEQFFPESLLGDVFWDEPLLATNQSCFVQTQPCPTPNLSAFVQYRDQPKVSFGEQSSRKGSNSNNMNKRMIAFLRKSLPLERNKVVECERERGFKHMISERMRRQRQRQCCFNLHAVLPHGTKTDNNSVVQTAAKEIQRLQGCKEELERKNFVLEKNVEVIDRRKQIQYLRVPCPTSGIDSTVETLKLLKDYGIDTKSVKSNFSQQELFLVLEIESEIAGTDVEKAIQRALALCLVEEK*