AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0542530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0542530 MtrunA17_Chr4g0033401 95.602 523 16 2 1 519 1 520 0.0 1028
Msa0542530 MtrunA17_Chr4g0033321 95.985 523 13 3 1 519 1 519 0.0 1028
Msa0542530 MtrunA17_Chr4g0033331 89.648 512 51 1 1 512 5 514 0.0 938
Msa0542530 MtrunA17_Chr4g0033311 75.294 510 124 1 5 514 9 516 0.0 830
Msa0542530 MtrunA17_Chr3g0088441 73.684 513 131 2 6 514 3 515 0.0 814
Msa0542530 MtrunA17_Chr2g0329201 71.343 499 141 1 13 511 9 505 0.0 758
Msa0542530 MtrunA17_Chr6g0482131 69.643 504 147 1 8 511 13 510 0.0 741
Msa0542530 MtrunA17_Chr2g0329191 71.875 480 134 1 32 511 29 507 0.0 739
Msa0542530 MtrunA17_Chr4g0033191 68.267 479 152 0 33 511 39 517 0.0 719
Msa0542530 MtrunA17_Chr6g0482071 69.375 480 147 0 37 516 37 516 0.0 713
Msa0542530 MtrunA17_Chr4g0033341 65.098 510 172 3 3 511 37 541 0.0 713
Msa0542530 MtrunA17_Chr4g0033201 65.476 504 171 1 8 511 10 510 0.0 712
Msa0542530 MtrunA17_Chr4g0033271 65.878 507 169 2 6 511 6 509 0.0 712
Msa0542530 MtrunA17_Chr6g0482081 67.723 505 156 2 8 511 13 511 0.0 701
Msa0542530 MtrunA17_Chr2g0329321 63.992 511 157 2 1 511 3 486 0.0 691
Msa0542530 MtrunA17_Chr4g0033261 62.231 511 163 3 6 514 12 494 0.0 650
Msa0542530 MtrunA17_Chr4g0033231 61.277 501 164 3 13 511 19 491 0.0 634
Msa0542530 MtrunA17_Chr5g0430451 61.914 512 160 2 9 511 11 496 0.0 623
Msa0542530 MtrunA17_Chr4g0010501 61.134 476 179 3 36 511 23 492 0.0 611
Msa0542530 MtrunA17_Chr4g0006111 56.175 502 180 7 22 511 50 523 0.0 562
Msa0542530 MtrunA17_Chr3g0087261 54.348 506 225 4 7 511 7 507 0.0 561
Msa0542530 MtrunA17_Chr3g0087271 52.652 509 235 5 4 511 5 508 0.0 538
Msa0542530 MtrunA17_Chr4g0006131 56.619 491 174 9 37 516 42 504 0.0 536
Msa0542530 MtrunA17_Chr4g0043091 52.465 507 229 6 9 511 3 501 0.0 532
Msa0542530 MtrunA17_Chr6g0482941 47.953 513 254 7 3 514 13 513 8.44e-169 487
Msa0542530 MtrunA17_Chr4g0042171 49.606 508 244 6 10 514 3 501 1.07e-168 487
Msa0542530 MtrunA17_Chr6g0482951 47.368 513 257 7 3 514 13 513 7.44e-168 484
Msa0542530 MtrunA17_Chr2g0301231 58.273 417 137 6 105 511 1 390 8.94e-165 472
Msa0542530 MtrunA17_Chr3g0090111 49.053 475 228 5 37 511 19 479 6.39e-159 461
Msa0542530 MtrunA17_Chr3g0090131 48.950 476 230 4 36 511 20 482 1.56e-158 460
Msa0542530 MtrunA17_Chr4g0074561 47.817 481 234 5 42 513 40 512 3.46e-156 455
Msa0542530 MtrunA17_Chr4g0074551 47.500 480 246 5 42 519 55 530 3.08e-153 449
Msa0542530 MtrunA17_Chr2g0288071 45.857 519 267 7 4 517 37 546 3.28e-151 443
Msa0542530 MtrunA17_Chr4g0006971 46.708 486 243 7 36 519 26 497 1.66e-147 433
Msa0542530 MtrunA17_Chr2g0317841 44.559 487 257 7 37 518 32 510 3.31e-147 432
Msa0542530 MtrunA17_Chr7g0242681 47.047 491 229 9 37 518 38 506 9.69e-147 431
Msa0542530 MtrunA17_Chr2g0317821 45.380 487 253 7 37 518 32 510 1.03e-146 431
Msa0542530 MtrunA17_Chr7g0242691 45.773 485 247 6 36 518 30 500 3.53e-146 429
Msa0542530 MtrunA17_Chr2g0288021 43.444 511 278 8 6 512 5 508 1.70e-141 417
Msa0542530 MtrunA17_Chr1g0195611 45.791 487 236 8 32 511 22 487 5.87e-139 411
Msa0542530 MtrunA17_Chr5g0428831 45.287 488 238 9 32 511 22 488 3.13e-137 406
Msa0542530 MtrunA17_Chr3g0087201 55.589 331 142 2 181 511 17 342 7.07e-132 386
Msa0542530 MtrunA17_Chr5g0418871 59.197 299 119 2 56 354 2 297 1.66e-128 378
Msa0542530 MtrunA17_Chr7g0234111 44.351 478 246 6 38 514 76 534 3.55e-128 384
Msa0542530 MtrunA17_Chr7g0234091 42.797 479 252 5 38 514 76 534 1.39e-125 377
Msa0542530 MtrunA17_Chr7g0234131 42.620 481 255 6 38 516 76 537 1.61e-124 375
Msa0542530 MtrunA17_Chr1g0195641 48.338 391 181 6 39 423 29 404 2.53e-119 357
Msa0542530 MtrunA17_Chr7g0230651 43.272 379 191 5 84 458 18 376 1.94e-100 308
Msa0542530 MtrunA17_Chr3g0087191 77.931 145 32 0 36 180 21 165 5.84e-82 252
Msa0542530 MtrunA17_Chr7g0236731 48.000 250 123 2 125 373 27 270 2.44e-65 213
Msa0542530 MtrunA17_Chr7g0227561 40.146 274 123 5 38 307 47 283 1.19e-62 207
Msa0542530 MtrunA17_Chr4g0068871 25.467 428 234 12 101 516 183 537 9.48e-29 120
Msa0542530 MtrunA17_Chr8g0352881 39.394 132 67 2 237 358 24 152 6.88e-25 100
Msa0542530 MtrunA17_Chr8g0352881 55.556 54 23 1 46 99 7 59 1.63e-13 68.6
Msa0542530 MtrunA17_Chr5g0418861 50.549 91 44 1 423 512 1 91 6.81e-24 95.9
Msa0542530 MtrunA17_Chr7g0227591 53.704 54 25 0 460 513 14 67 3.42e-13 64.7
Msa0542530 MtrunA17_Chr2g0288031 47.458 59 30 1 188 246 3 60 3.38e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0542530 AT5G44640.1 53.981 515 225 5 1 514 1 504 0.0 577
Msa0542530 AT2G44450.1 55.717 481 208 2 37 517 31 506 0.0 570
Msa0542530 AT5G42260.1 54.528 508 219 5 1 507 1 497 0.0 568
Msa0542530 AT2G44480.1 54.583 480 217 1 36 515 34 512 0.0 562
Msa0542530 AT2G44480.3 54.583 480 217 1 36 515 65 543 0.0 562
Msa0542530 AT3G60130.1 55.462 476 206 3 37 511 30 500 0.0 557
Msa0542530 AT2G25630.1 51.992 502 218 4 14 514 7 486 0.0 538
Msa0542530 AT5G24550.1 53.445 479 218 5 37 511 32 509 0.0 531
Msa0542530 AT3G60140.1 49.900 499 239 6 16 511 12 502 0.0 523
Msa0542530 AT5G24540.1 51.663 511 233 8 9 511 5 509 0.0 521
Msa0542530 AT1G51470.1 53.862 479 207 7 36 512 44 510 3.43e-180 516
Msa0542530 AT1G26560.1 52.075 482 221 6 37 513 32 508 2.73e-178 511
Msa0542530 AT1G47600.1 53.236 479 210 7 36 512 44 510 1.82e-177 509
Msa0542530 AT1G47600.2 53.236 479 209 7 36 512 44 509 3.54e-177 508
Msa0542530 AT3G60120.1 51.452 482 223 7 36 511 13 489 5.22e-174 501
Msa0542530 AT2G44460.1 48.263 518 255 7 1 515 1 508 3.98e-171 496
Msa0542530 AT5G54570.1 49.485 485 228 4 36 511 29 505 1.10e-170 493
Msa0542530 AT3G18080.1 50.617 486 230 7 32 515 34 511 1.22e-170 492
Msa0542530 AT2G44470.3 45.873 521 270 6 1 518 1 512 1.95e-170 494
Msa0542530 AT2G44490.1 49.190 494 244 6 29 517 7 498 2.02e-169 491
Msa0542530 AT3G18070.3 49.184 490 229 9 28 514 21 493 3.77e-166 480
Msa0542530 AT3G18070.1 49.388 490 234 9 28 514 21 499 2.42e-165 478
Msa0542530 AT5G25980.2 48.263 518 256 9 6 517 19 530 8.93e-164 476
Msa0542530 AT2G44480.2 52.555 411 194 1 105 515 1 410 2.28e-163 470
Msa0542530 AT3G60130.2 54.106 414 184 3 99 511 40 448 2.40e-163 471
Msa0542530 AT5G36890.2 49.582 478 228 4 38 514 17 482 8.42e-159 461
Msa0542530 AT5G36890.1 49.582 478 228 4 38 514 17 482 1.01e-158 461
Msa0542530 AT3G09260.1 48.333 480 241 6 37 512 35 511 4.65e-158 460
Msa0542530 AT2G32860.2 45.436 482 260 3 36 514 93 574 8.64e-157 460
Msa0542530 AT3G60130.3 53.140 414 177 4 99 511 40 437 2.24e-156 453
Msa0542530 AT5G26000.1 46.654 523 259 10 8 518 4 518 4.73e-156 456
Msa0542530 AT2G32860.1 45.228 482 260 4 36 514 93 573 2.66e-155 456
Msa0542530 AT5G24540.2 50.000 456 215 7 9 457 5 454 2.48e-153 446
Msa0542530 AT3G03640.1 46.362 481 252 4 36 511 33 512 2.49e-153 448
Msa0542530 AT1G75940.1 47.531 486 239 9 37 511 37 517 7.69e-153 447
Msa0542530 AT3G21370.1 46.170 483 250 8 36 511 32 511 2.46e-151 443
Msa0542530 AT1G52400.3 45.331 514 268 10 8 514 11 518 2.62e-151 443
Msa0542530 AT1G52400.1 45.331 514 268 10 8 514 11 518 2.62e-151 443
Msa0542530 AT1G66280.1 45.472 519 263 10 1 511 4 510 9.12e-150 439
Msa0542530 AT5G28510.1 45.732 492 255 7 31 513 33 521 1.53e-149 439
Msa0542530 AT5G54570.3 48.492 431 206 3 36 458 29 451 1.64e-147 431
Msa0542530 AT1G61820.1 46.612 487 252 7 29 511 22 504 6.63e-147 431
Msa0542530 AT2G44470.1 44.880 459 242 5 1 457 1 450 8.85e-147 429
Msa0542530 AT1G66270.1 46.377 483 247 8 37 513 36 512 1.24e-146 431
Msa0542530 AT3G60140.2 48.900 409 199 5 16 422 12 412 2.88e-145 425
Msa0542530 AT1G02850.2 47.158 475 234 7 38 511 27 485 3.16e-145 427
Msa0542530 AT1G66270.2 46.170 483 246 9 37 513 36 510 1.23e-143 423
Msa0542530 AT5G25980.3 47.473 455 231 6 6 458 19 467 6.33e-143 419
Msa0542530 AT5G25980.1 47.473 455 231 6 6 458 19 467 6.33e-143 419
Msa0542530 AT4G21760.2 43.689 515 277 7 1 511 16 521 2.97e-142 421
Msa0542530 AT1G02850.5 45.181 498 233 8 38 511 27 508 6.78e-141 416
Msa0542530 AT1G02850.4 45.291 499 232 9 38 511 27 509 2.95e-139 412
Msa0542530 AT5G54570.2 48.077 416 199 4 105 511 1 408 1.22e-137 405
Msa0542530 AT1G60090.2 44.099 483 246 9 37 515 24 486 4.69e-137 406
Msa0542530 AT2G44480.4 60.256 312 122 2 36 347 65 374 9.28e-137 401
Msa0542530 AT1G61810.1 45.992 474 248 7 42 511 38 507 1.43e-136 405
Msa0542530 AT4G27830.1 42.095 506 266 7 9 511 3 484 1.46e-136 405
Msa0542530 AT5G26000.2 45.987 461 231 8 8 458 4 456 1.39e-135 400
Msa0542530 AT4G21760.1 42.718 515 272 8 1 511 16 511 3.44e-135 402
Msa0542530 AT1G02850.1 45.684 475 214 8 38 511 27 458 4.25e-135 400
Msa0542530 AT2G44450.2 51.389 360 170 2 158 517 1 355 3.89e-134 393
Msa0542530 AT1G60090.1 42.799 493 245 10 37 515 24 493 4.80e-133 396
Msa0542530 AT1G61810.3 46.204 461 240 7 42 498 38 494 2.27e-132 395
Msa0542530 AT4G22100.1 44.351 478 244 9 38 511 23 482 2.79e-132 394
Msa0542530 AT3G62750.1 41.837 490 253 8 26 511 12 473 5.32e-129 385
Msa0542530 AT3G62750.2 41.837 490 253 8 26 511 38 499 1.81e-128 385
Msa0542530 AT4G27820.1 43.542 480 248 7 36 511 22 482 2.20e-128 384
Msa0542530 AT1G45191.4 43.451 481 244 9 38 514 30 486 5.88e-128 383
Msa0542530 AT2G44470.2 44.444 405 214 5 1 403 1 396 2.17e-126 375
Msa0542530 AT1G52400.2 44.298 456 242 9 8 457 11 460 6.60e-126 376
Msa0542530 AT1G45191.6 42.041 490 249 9 38 514 30 497 4.38e-124 373
Msa0542530 AT1G45191.2 42.204 481 256 9 38 514 30 492 7.25e-124 372
Msa0542530 AT3G62740.1 40.695 489 265 10 26 511 12 478 1.13e-122 369
Msa0542530 AT1G45191.1 41.600 500 249 10 38 514 30 509 1.61e-122 370
Msa0542530 AT5G48375.1 45.638 447 171 9 33 476 34 411 6.07e-121 362
Msa0542530 AT1G02850.3 46.569 408 185 6 38 422 27 424 1.15e-120 362
Msa0542530 AT3G18070.4 51.796 334 154 5 28 361 21 347 7.14e-120 358
Msa0542530 AT1G60270.1 48.256 344 167 5 37 377 24 359 1.43e-111 336
Msa0542530 AT3G62750.4 41.324 438 226 7 26 460 38 447 2.62e-111 338
Msa0542530 AT2G44460.2 48.209 363 184 4 156 515 1 362 1.42e-110 336
Msa0542530 AT1G51490.1 49.432 352 161 7 44 392 27 364 1.08e-109 333
Msa0542530 AT1G61810.5 44.282 411 221 7 105 511 1 407 2.27e-109 332
Msa0542530 AT1G61810.4 45.714 385 202 6 42 422 38 419 8.25e-109 331
Msa0542530 AT3G62740.2 41.549 426 225 9 34 457 20 423 2.76e-108 329
Msa0542530 AT4G27830.2 41.707 410 222 5 105 511 1 396 8.76e-107 325
Msa0542530 AT1G45191.5 48.512 336 161 5 38 370 30 356 3.48e-106 323
Msa0542530 AT1G45191.3 48.512 336 161 5 38 370 30 356 5.00e-106 323
Msa0542530 AT3G62750.7 45.946 333 164 4 26 358 12 328 7.05e-104 316
Msa0542530 AT3G62750.8 45.946 333 164 4 26 358 12 328 7.05e-104 316
Msa0542530 AT3G62750.3 45.946 333 164 4 26 358 38 354 5.20e-103 316
Msa0542530 AT2G44480.5 48.013 302 156 1 214 515 6 306 4.69e-101 307
Msa0542530 AT3G18070.2 47.432 331 165 6 188 515 99 423 2.56e-100 309
Msa0542530 AT3G18070.2 56.944 72 30 1 28 99 21 91 2.35e-19 90.9
Msa0542530 AT1G61820.3 44.932 365 194 6 151 511 4 365 5.93e-100 306
Msa0542530 AT4G27820.2 42.092 411 220 6 105 511 1 397 1.19e-99 307
Msa0542530 AT4G27820.3 42.092 411 220 6 105 511 1 397 1.19e-99 307
Msa0542530 AT3G62750.6 39.659 411 221 7 105 511 1 388 4.06e-97 300
Msa0542530 AT3G62750.5 39.659 411 221 7 105 511 1 388 4.06e-97 300
Msa0542530 AT3G18070.7 46.835 316 159 6 202 514 1 310 3.08e-93 287
Msa0542530 AT1G61810.2 52.075 265 125 2 42 306 38 300 6.32e-90 278
Msa0542530 AT1G60270.2 47.101 276 139 4 105 377 1 272 7.48e-82 257
Msa0542530 AT3G18070.5 45.607 239 126 3 188 424 99 335 2.19e-67 221
Msa0542530 AT3G18070.5 56.944 72 30 1 28 99 21 91 1.13e-19 90.9
Msa0542530 AT3G18070.6 44.643 224 120 3 202 423 1 222 1.44e-60 199
Msa0542530 AT5G16580.1 36.025 322 177 7 152 468 2 299 1.31e-48 170
Msa0542530 AT3G06510.2 24.661 442 256 14 100 516 159 548 3.16e-29 122
Msa0542530 AT3G06510.1 24.942 429 237 14 100 516 159 514 2.18e-28 119

Find 101 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTAATTATATAGGTTTA+TGG 0.150754 4_1:-27628925 Msa0542530:intron
TTAATTTATATTACTGAAAA+TGG 0.152248 4_1:-27628361 Msa0542530:intron
TTTCCAGAAGGATTCATATT+TGG 0.213392 4_1:-27630399 Msa0542530:CDS
CACCAGGTCGATGCTCTTCT+TGG 0.247168 4_1:-27629268 Msa0542530:CDS
AATTGCACCGGTGGTGATTC+TGG 0.263758 4_1:-27629236 Msa0542530:CDS
GAATCACCACCGGTGCAATT+TGG 0.269114 4_1:+27629239 None:intergenic
GTGGAAGGGGACCAAGTATA+TGG 0.275314 4_1:-27630332 Msa0542530:CDS
TGATAATGTGCAACTAAATA+TGG 0.279620 4_1:+27629206 None:intergenic
ATCGCTTGAGGAATCTCTTA+TGG 0.285631 4_1:-27628197 Msa0542530:CDS
ACTTTCAGGTTGCTTCAAAT+TGG 0.301411 4_1:-27628450 Msa0542530:intron
CAACAACCTCATTAATGAAT+TGG 0.310357 4_1:-27630103 Msa0542530:CDS
GAACTTTGCTTTAAAACATT+TGG 0.321295 4_1:-27629368 Msa0542530:CDS
AGCAAAGTTCCGCATAATCT+TGG 0.329107 4_1:+27629381 None:intergenic
AAGGGTTATACCGTAAGATT+TGG 0.344341 4_1:-27627960 Msa0542530:CDS
TTTCAGTAATATAAATTAAA+GGG 0.356229 4_1:+27628365 None:intergenic
AGATAACAAACTTGATATAA+AGG 0.370220 4_1:-27629075 Msa0542530:CDS
GTGCCAAATATGAATCCTTC+TGG 0.376797 4_1:+27630396 None:intergenic
CCTCCACTTAGTTTCCCATC+TGG 0.378131 4_1:+27630153 None:intergenic
AGAGCTACAGACTTTATGTT+TGG 0.379843 4_1:-27629044 Msa0542530:CDS
CAAACTCTAGAAGATGAATA+TGG 0.381482 4_1:-27629534 Msa0542530:CDS
ATATATTATCTATATGTAAT+TGG 0.387249 4_1:-27627727 Msa0542530:three_prime_UTR
TGCAAATAGGTTCATTGAAT+AGG 0.387545 4_1:-27630428 Msa0542530:CDS
CTCCACTTAGTTTCCCATCT+GGG 0.388126 4_1:+27630154 None:intergenic
TATTCATCTTCTAGAGTTTG+AGG 0.401353 4_1:+27629537 None:intergenic
AACACTGCACCACTGCAAAT+AGG 0.401953 4_1:-27630441 Msa0542530:CDS
AGACAACAAGAGAGAGAATA+GGG 0.407071 4_1:+27630512 None:intergenic
GTCTCTCAAAAGGGTGTGAT+AGG 0.412344 4_1:-27629134 Msa0542530:CDS
ATACTTTGCATGGTCTTTGT+TGG 0.413214 4_1:-27628000 Msa0542530:CDS
TAGACAACAAGAGAGAGAAT+AGG 0.421597 4_1:+27630511 None:intergenic
AGTTTACAAAAGAGCAATCT+AGG 0.421822 4_1:-27628839 Msa0542530:CDS
CAAATATCAAGTCTCTCAAA+AGG 0.425107 4_1:-27629144 Msa0542530:CDS
TGGTAACCTTGTTCTGACTA+AGG 0.441298 4_1:+27628863 None:intergenic
AGAAAGTCTCGAATTGCTCT+TGG 0.442143 4_1:+27628415 None:intergenic
TGACTCGTATCATCGCTATA+AGG 0.443207 4_1:-27630247 Msa0542530:CDS
ATAATGATAATCAACTCTAT+AGG 0.445122 4_1:+27628171 None:intergenic
ATTAATGAATTGGTGGCAAA+TGG 0.457316 4_1:-27630093 Msa0542530:intron
CAGGGCTGGTGTAAATGTAA+AGG 0.460153 4_1:-27628024 Msa0542530:CDS
TGGATAATCTCCATTGGCTA+AGG 0.461168 4_1:+27628899 None:intergenic
AGTGAATCTGTTAGGTAGCT+AGG 0.464690 4_1:+27628729 None:intergenic
AAAAGATATCAAAAGCTCTC+AGG 0.470702 4_1:-27627906 Msa0542530:CDS
GGTTTGCCATTGCTTAATTG+AGG 0.479254 4_1:+27628750 None:intergenic
AACGAGAAAGTGAATCTGTT+AGG 0.479548 4_1:+27628721 None:intergenic
AGAAAGAAAGAATGAATGAA+TGG 0.479959 4_1:+27630627 None:intergenic
AGGGCTGGTGTAAATGTAAA+GGG 0.484456 4_1:-27628023 Msa0542530:CDS
CTACAGACTTTATGTTTGGA+TGG 0.487340 4_1:-27629040 Msa0542530:intron
TTGCCACCAATTCATTAATG+AGG 0.489921 4_1:+27630097 None:intergenic
AACACTTACATTTAGACCAA+TGG 0.493905 4_1:+27628557 None:intergenic
TCATTTATTCTATCTTAATG+AGG 0.494555 4_1:-27628146 Msa0542530:CDS
CAACCTCATTAATGAATTGG+TGG 0.495036 4_1:-27630100 Msa0542530:CDS
TGGAAGGGGACCAAGTATAT+GGG 0.495166 4_1:-27630331 Msa0542530:CDS
TGGGTATGCAAATGGTGAAA+TGG 0.498834 4_1:-27629291 Msa0542530:CDS
ATGTGCAACTAAATATGGTT+CGG 0.507608 4_1:+27629211 None:intergenic
AAAGGTCATTCCAAATCTTA+CGG 0.508922 4_1:+27627950 None:intergenic
CCATGGACATACAGTCAAGA+TGG 0.510839 4_1:-27629311 Msa0542530:CDS
TTCATTATTACTTTAACAGA+AGG 0.515348 4_1:-27630477 Msa0542530:CDS
GGATAATCTCCATTGGCTAA+GGG 0.515711 4_1:+27628900 None:intergenic
CATCGCTATAAGGAAGATGT+TGG 0.525320 4_1:-27630237 Msa0542530:CDS
AGTCAAGATGGGTATGCAAA+TGG 0.528018 4_1:-27629299 Msa0542530:CDS
TTGGAGATAGAGTTAAACAT+TGG 0.530071 4_1:-27629349 Msa0542530:CDS
TGCAGCAAGTGAAGGTGGAA+GGG 0.535237 4_1:-27630346 Msa0542530:CDS
GCAGCAAGTGAAGGTGGAAG+GGG 0.542357 4_1:-27630345 Msa0542530:CDS
TCTTCTGCATATCAATATGA+AGG 0.549189 4_1:-27630369 Msa0542530:CDS
GTGCAGCAAGTGAAGGTGGA+AGG 0.549707 4_1:-27630347 Msa0542530:CDS
TATTTACATACAATTTCAGA+TGG 0.552486 4_1:+27627747 None:intergenic
AAAATCAGTGATGGAAACAA+TGG 0.553564 4_1:-27630282 Msa0542530:CDS
TTTATGGATCCCTTAGCCAA+TGG 0.559848 4_1:-27628909 Msa0542530:CDS
TGGTCTAGAATACTCCCAGA+TGG 0.563550 4_1:-27630168 Msa0542530:CDS
TGATCCATCGTTATCGCTTG+AGG 0.566394 4_1:-27628209 Msa0542530:CDS
CCATCTTGACTGTATGTCCA+TGG 0.566884 4_1:+27629311 None:intergenic
AAATATCAAGTCTCTCAAAA+GGG 0.567104 4_1:-27629143 Msa0542530:CDS
ATCAAAAGCTCTCAGGATTG+TGG 0.567379 4_1:-27627899 Msa0542530:CDS
GTGAAAGTATCCCATATACT+TGG 0.569026 4_1:+27630321 None:intergenic
CTCCCAGATGGGAAACTAAG+TGG 0.570318 4_1:-27630156 Msa0542530:CDS
GCAAATGGTGAAATGGCACC+AGG 0.576425 4_1:-27629284 Msa0542530:CDS
TTCAATGAACCTATTTGCAG+TGG 0.584960 4_1:+27630432 None:intergenic
TCAAGTTTGTTATCTGAGAG+TGG 0.587930 4_1:+27629083 None:intergenic
AATCATGAAGGATATGAACT+TGG 0.589040 4_1:-27630214 Msa0542530:CDS
GGAAGATGTTGGAATCATGA+AGG 0.590169 4_1:-27630226 Msa0542530:CDS
AACCAAGAAGAGCATCGACC+TGG 0.592148 4_1:+27629266 None:intergenic
TGGTTGAATCCAAATTGCAC+CGG 0.592901 4_1:-27629248 Msa0542530:CDS
AGTGGAGGAATTAATCAAGA+AGG 0.596031 4_1:-27630138 Msa0542530:CDS
GAAGGTGCAGCAAGTGAAGG+TGG 0.597164 4_1:-27630351 Msa0542530:CDS
ACTTCTAGCTCATGCAGCAG+TGG 0.599488 4_1:-27629183 Msa0542530:CDS
CATGGACATACAGTCAAGAT+GGG 0.602027 4_1:-27629310 Msa0542530:CDS
AAAAGATTTCCAAGATTATG+CGG 0.603350 4_1:-27629390 Msa0542530:CDS
TATGAAGGTGCAGCAAGTGA+AGG 0.604375 4_1:-27630354 Msa0542530:CDS
GTATCACTCACATGATGAGA+GGG 0.606257 4_1:+27629499 None:intergenic
GGTCTAGAATACTCCCAGAT+GGG 0.607544 4_1:-27630167 Msa0542530:CDS
GGATAACTTTGAATGAACCA+TGG 0.616263 4_1:-27629328 Msa0542530:CDS
TGTATCACTCACATGATGAG+AGG 0.618007 4_1:+27629498 None:intergenic
GGGTGTGATAGGTATAACAT+TGG 0.623641 4_1:-27629123 Msa0542530:CDS
ATGTAAAGGGATACTTTGCA+TGG 0.626161 4_1:-27628010 Msa0542530:CDS
TTCGGTTCCAGAATCACCAC+CGG 0.630837 4_1:+27629229 None:intergenic
GTGATTGCAGTCGAACGTGA+TGG 0.630848 4_1:-27628585 Msa0542530:intron
GATGCACCTCAATTAAGCAA+TGG 0.645151 4_1:-27628756 Msa0542530:CDS
GAGCAATCTAGGCTAGTGAG+TGG 0.669773 4_1:-27628828 Msa0542530:CDS
TTGAATCCAAATTGCACCGG+TGG 0.672179 4_1:-27629245 Msa0542530:CDS
GATTCCTCAAGCGATAACGA+TGG 0.674001 4_1:+27628205 None:intergenic
TGAGAGCCTTAGTCAGAACA+AGG 0.678452 4_1:-27628869 Msa0542530:CDS
ACTCTAGAAGATGAATATGG+CGG 0.692578 4_1:-27629531 Msa0542530:CDS
CCAGATGGGAAACTAAGTGG+AGG 0.696893 4_1:-27630153 Msa0542530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTATTTTTTATATT+TGG - chr4_1:27628682-27628701 Msa0542530:intron 0.0%
!! ATATTATATATTTAAATTAA+TGG - chr4_1:27629728-27629747 Msa0542530:intron 0.0%
!!! ATTTCTTTGTTTAATTATAT+AGG - chr4_1:27629420-27629439 Msa0542530:intron 10.0%
!! AAATTAGTAATTAATGAATT+TGG - chr4_1:27629872-27629891 Msa0542530:intron 10.0%
!! TTTCAGTAATATAAATTAAA+GGG + chr4_1:27629990-27630009 None:intergenic 10.0%
!!! TTTTCAGTAATATAAATTAA+AGG + chr4_1:27629991-27630010 None:intergenic 10.0%
!! TTAATTTATATTACTGAAAA+TGG - chr4_1:27629991-27630010 Msa0542530:intron 10.0%
!! ATATATTATCTATATGTAAT+TGG - chr4_1:27630625-27630644 Msa0542530:five_prime_UTR 10.0%
!! AAGAAGAAAACTTAAATAAT+TGG - chr4_1:27628375-27628394 Msa0542530:CDS 15.0%
!! TAGCCAAATAATATATTAAT+GGG + chr4_1:27628422-27628441 None:intergenic 15.0%
!!! AAACAATTTTGTTGTTTTAA+AGG - chr4_1:27628446-27628465 Msa0542530:intron 15.0%
!!! AACAATTTTGTTGTTTTAAA+GGG - chr4_1:27628447-27628466 Msa0542530:intron 15.0%
!! TGTTTAATTATATAGGTTTA+TGG - chr4_1:27629427-27629446 Msa0542530:intron 15.0%
!! GATATAAAATAGATAGTAAT+AGG + chr4_1:27629679-27629698 None:intergenic 15.0%
!!! TTTCTTTTAATCTAAATACA+GGG - chr4_1:27630310-27630329 Msa0542530:CDS 15.0%
!!! AATAATTGTTTTTACAACTA+TGG + chr4_1:27630547-27630566 None:intergenic 15.0%
!! TTCATTATTACTTTAACAGA+AGG - chr4_1:27627875-27627894 Msa0542530:CDS 20.0%
!!! TTAAGTTTTCTTCTTCTTTT+GGG + chr4_1:27628370-27628389 None:intergenic 20.0%
!!! TTTAAGTTTTCTTCTTCTTT+TGG + chr4_1:27628371-27628390 None:intergenic 20.0%
!! TACCCCATTAATATATTATT+TGG - chr4_1:27628416-27628435 Msa0542530:CDS 20.0%
!! AGCCAAATAATATATTAATG+GGG + chr4_1:27628421-27628440 None:intergenic 20.0%
!! GTAGCCAAATAATATATTAA+TGG + chr4_1:27628423-27628442 None:intergenic 20.0%
!!! ATTTGTAACACTTTTTCATT+GGG - chr4_1:27628787-27628806 Msa0542530:CDS 20.0%
!! AGATAACAAACTTGATATAA+AGG - chr4_1:27629277-27629296 Msa0542530:CDS 20.0%
!!! AATTTGGTTATTTTACTTTC+AGG - chr4_1:27629888-27629907 Msa0542530:intron 20.0%
!! ATAATGATAATCAACTCTAT+AGG + chr4_1:27630184-27630203 None:intergenic 20.0%
!! TCATTTATTCTATCTTAATG+AGG - chr4_1:27630206-27630225 Msa0542530:CDS 20.0%
!!! CTTTCTTTTAATCTAAATAC+AGG - chr4_1:27630309-27630328 Msa0542530:CDS 20.0%
!! TATTTACATACAATTTCAGA+TGG + chr4_1:27630608-27630627 None:intergenic 20.0%
! AGAAAGAAAGAATGAATGAA+TGG + chr4_1:27627728-27627747 None:intergenic 25.0%
!!! CATACAGATTTTCTATTTCT+TGG - chr4_1:27628164-27628183 Msa0542530:CDS 25.0%
!! TAAGTTTTCTTCTTCTTTTG+GGG + chr4_1:27628369-27628388 None:intergenic 25.0%
! TTAGAATATGCTAAAATCAG+CGG + chr4_1:27628736-27628755 None:intergenic 25.0%
!!! CAATGAAAAAGTGTTACAAA+TGG + chr4_1:27628788-27628807 None:intergenic 25.0%
!!! CATTTGTAACACTTTTTCAT+TGG - chr4_1:27628786-27628805 Msa0542530:CDS 25.0%
! AAAAGATTTCCAAGATTATG+CGG - chr4_1:27628962-27628981 Msa0542530:intron 25.0%
!!! GAACTTTGCTTTAAAACATT+TGG - chr4_1:27628984-27629003 Msa0542530:intron 25.0%
!! TGATAATGTGCAACTAAATA+TGG + chr4_1:27629149-27629168 None:intergenic 25.0%
! AAATATCAAGTCTCTCAAAA+GGG - chr4_1:27629209-27629228 Msa0542530:CDS 25.0%
! ACTTATTCCAAATTAACACA+AGG - chr4_1:27629372-27629391 Msa0542530:CDS 25.0%
! TATATCTCCTTGTGTTAATT+TGG + chr4_1:27629382-27629401 None:intergenic 25.0%
!!! AGTGGTTCATTTGATTTTAT+TGG - chr4_1:27629542-27629561 Msa0542530:CDS 25.0%
! GCAACAACTTAAATGTTATT+TGG - chr4_1:27629840-27629859 Msa0542530:intron 25.0%
!!! AACTTTTTCATAATGTGTGT+AGG - chr4_1:27630105-27630124 Msa0542530:CDS 25.0%
! ACCTTTGTAGATTACAAAAA+TGG - chr4_1:27630419-27630438 Msa0542530:CDS 25.0%
!!! ACCATTTTTGTAATCTACAA+AGG + chr4_1:27630423-27630442 None:intergenic 25.0%
!! AATTTTGTTGCACTGTTAAA+TGG + chr4_1:27630513-27630532 None:intergenic 25.0%
TGCAAATAGGTTCATTGAAT+AGG - chr4_1:27627924-27627943 Msa0542530:CDS 30.0%
! AATAGGAAAGATTTTCCAGA+AGG - chr4_1:27627941-27627960 Msa0542530:CDS 30.0%
TTTCCAGAAGGATTCATATT+TGG - chr4_1:27627953-27627972 Msa0542530:CDS 30.0%
TCTTCTGCATATCAATATGA+AGG - chr4_1:27627983-27628002 Msa0542530:CDS 30.0%
TATCCTCAAAAAATCAGTGA+TGG - chr4_1:27628061-27628080 Msa0542530:intron 30.0%
!!! TTTCCATCACTGATTTTTTG+AGG + chr4_1:27628067-27628086 None:intergenic 30.0%
! AAAATCAGTGATGGAAACAA+TGG - chr4_1:27628070-27628089 Msa0542530:intron 30.0%
AATCATGAAGGATATGAACT+TGG - chr4_1:27628138-27628157 Msa0542530:intron 30.0%
CAACAACCTCATTAATGAAT+TGG - chr4_1:27628249-27628268 Msa0542530:intron 30.0%
! ATTAATGAATTGGTGGCAAA+TGG - chr4_1:27628259-27628278 Msa0542530:intron 30.0%
!! TATTCATCTTCTAGAGTTTG+AGG + chr4_1:27628818-27628837 None:intergenic 30.0%
! CAAACTCTAGAAGATGAATA+TGG - chr4_1:27628818-27628837 Msa0542530:CDS 30.0%
TTGGAGATAGAGTTAAACAT+TGG - chr4_1:27629003-27629022 Msa0542530:intron 30.0%
ATGTGCAACTAAATATGGTT+CGG + chr4_1:27629144-27629163 None:intergenic 30.0%
!! CTTTTGAGAGACTTGATATT+TGG + chr4_1:27629210-27629229 None:intergenic 30.0%
CAAATATCAAGTCTCTCAAA+AGG - chr4_1:27629208-27629227 Msa0542530:CDS 30.0%
!!! GTTTTTTGGATAATCTCCAT+TGG + chr4_1:27629462-27629481 None:intergenic 30.0%
! GATTGCTCTTTTGTAAACTT+TGG + chr4_1:27629512-27629531 None:intergenic 30.0%
AGTTTACAAAAGAGCAATCT+AGG - chr4_1:27629513-27629532 Msa0542530:CDS 30.0%
! TTTTCATGTAAGCATCCATT+TGG - chr4_1:27629653-27629672 Msa0542530:intron 30.0%
ATAGATAGTAATAGGCCAAA+TGG + chr4_1:27629671-27629690 None:intergenic 30.0%
AACACTTACATTTAGACCAA+TGG + chr4_1:27629798-27629817 None:intergenic 30.0%
! TTGCATGTTGGAAATTTTGA+GGG + chr4_1:27629824-27629843 None:intergenic 30.0%
!! ACATTTAAGTTGTTGCATGT+TGG + chr4_1:27629836-27629855 None:intergenic 30.0%
AGCAAATCACTTTCATATCA+TGG - chr4_1:27630016-27630035 Msa0542530:intron 30.0%
! TTTTAATCTAAATACAGGGC+TGG - chr4_1:27630314-27630333 Msa0542530:CDS 30.0%
! CTTTGTTGGACAATTTTGAA+TGG - chr4_1:27630366-27630385 Msa0542530:CDS 30.0%
AAAGGTCATTCCAAATCTTA+CGG + chr4_1:27630405-27630424 None:intergenic 30.0%
AAAAGATATCAAAAGCTCTC+AGG - chr4_1:27630446-27630465 Msa0542530:CDS 30.0%
GATGACGATGACAAAAACAA+GGG + chr4_1:27627777-27627796 None:intergenic 35.0%
! TGATGACGATGACAAAAACA+AGG + chr4_1:27627778-27627797 None:intergenic 35.0%
AGACAACAAGAGAGAGAATA+GGG + chr4_1:27627843-27627862 None:intergenic 35.0%
TAGACAACAAGAGAGAGAAT+AGG + chr4_1:27627844-27627863 None:intergenic 35.0%
TTCAATGAACCTATTTGCAG+TGG + chr4_1:27627923-27627942 None:intergenic 35.0%
!! GTGAAAGTATCCCATATACT+TGG + chr4_1:27628034-27628053 None:intergenic 35.0%
AGTGGAGGAATTAATCAAGA+AGG - chr4_1:27628214-27628233 Msa0542530:CDS 35.0%
CAACCTCATTAATGAATTGG+TGG - chr4_1:27628252-27628271 Msa0542530:intron 35.0%
TTGCCACCAATTCATTAATG+AGG + chr4_1:27628258-27628277 None:intergenic 35.0%
!! TTATGCTTTGCATTTTAGCC+AGG - chr4_1:27628306-27628325 Msa0542530:intron 35.0%
!! TATGCTTTGCATTTTAGCCA+GGG - chr4_1:27628307-27628326 Msa0542530:intron 35.0%
! ACTCTAGAAGATGAATATGG+CGG - chr4_1:27628821-27628840 Msa0542530:CDS 35.0%
GGATAACTTTGAATGAACCA+TGG - chr4_1:27629024-27629043 Msa0542530:intron 35.0%
!! TCAAGTTTGTTATCTGAGAG+TGG + chr4_1:27629272-27629291 None:intergenic 35.0%
AGAGCTACAGACTTTATGTT+TGG - chr4_1:27629308-27629327 Msa0542530:CDS 35.0%
CTACAGACTTTATGTTTGGA+TGG - chr4_1:27629312-27629331 Msa0542530:CDS 35.0%
!!! ACTAAGGCTCTCATGTTTTT+TGG + chr4_1:27629476-27629495 None:intergenic 35.0%
AACGAGAAAGTGAATCTGTT+AGG + chr4_1:27629634-27629653 None:intergenic 35.0%
! GTTGCATGTTGGAAATTTTG+AGG + chr4_1:27629825-27629844 None:intergenic 35.0%
!! ACTTTCAGGTTGCTTCAAAT+TGG - chr4_1:27629902-27629921 Msa0542530:intron 35.0%
ATGTAAAGGGATACTTTGCA+TGG - chr4_1:27630342-27630361 Msa0542530:CDS 35.0%
ATACTTTGCATGGTCTTTGT+TGG - chr4_1:27630352-27630371 Msa0542530:CDS 35.0%
! GACAATTTTGAATGGCACAA+GGG - chr4_1:27630374-27630393 Msa0542530:CDS 35.0%
AAGGGTTATACCGTAAGATT+TGG - chr4_1:27630392-27630411 Msa0542530:CDS 35.0%
GTGCCAAATATGAATCCTTC+TGG + chr4_1:27627959-27627978 None:intergenic 40.0%
TGACTCGTATCATCGCTATA+AGG - chr4_1:27628105-27628124 Msa0542530:intron 40.0%
CATCGCTATAAGGAAGATGT+TGG - chr4_1:27628115-27628134 Msa0542530:intron 40.0%
GGAAGATGTTGGAATCATGA+AGG - chr4_1:27628126-27628145 Msa0542530:intron 40.0%
!! ATGCTTTGCATTTTAGCCAG+GGG - chr4_1:27628308-27628327 Msa0542530:intron 40.0%
ATATAGCCATCTCATGTCAC+AGG - chr4_1:27628755-27628774 Msa0542530:CDS 40.0%
GTATCACTCACATGATGAGA+GGG + chr4_1:27628856-27628875 None:intergenic 40.0%
TGTATCACTCACATGATGAG+AGG + chr4_1:27628857-27628876 None:intergenic 40.0%
AGCAAAGTTCCGCATAATCT+TGG + chr4_1:27628974-27628993 None:intergenic 40.0%
CATGGACATACAGTCAAGAT+GGG - chr4_1:27629042-27629061 Msa0542530:CDS 40.0%
AGTCAAGATGGGTATGCAAA+TGG - chr4_1:27629053-27629072 Msa0542530:CDS 40.0%
TGGGTATGCAAATGGTGAAA+TGG - chr4_1:27629061-27629080 Msa0542530:CDS 40.0%
TGGTTGAATCCAAATTGCAC+CGG - chr4_1:27629104-27629123 Msa0542530:CDS 40.0%
GGGTGTGATAGGTATAACAT+TGG - chr4_1:27629229-27629248 Msa0542530:CDS 40.0%
TTTATGGATCCCTTAGCCAA+TGG - chr4_1:27629443-27629462 Msa0542530:intron 40.0%
! GGATAATCTCCATTGGCTAA+GGG + chr4_1:27629455-27629474 None:intergenic 40.0%
! TGGATAATCTCCATTGGCTA+AGG + chr4_1:27629456-27629475 None:intergenic 40.0%
TGGTAACCTTGTTCTGACTA+AGG + chr4_1:27629492-27629511 None:intergenic 40.0%
GATGCACCTCAATTAAGCAA+TGG - chr4_1:27629596-27629615 Msa0542530:intron 40.0%
GGTTTGCCATTGCTTAATTG+AGG + chr4_1:27629605-27629624 None:intergenic 40.0%
AGTGAATCTGTTAGGTAGCT+AGG + chr4_1:27629626-27629645 None:intergenic 40.0%
! GAACGTGATGGAAAAACCAT+TGG - chr4_1:27629779-27629798 Msa0542530:intron 40.0%
AGAAAGTCTCGAATTGCTCT+TGG + chr4_1:27629940-27629959 None:intergenic 40.0%
ATCGCTTGAGGAATCTCTTA+TGG - chr4_1:27630155-27630174 Msa0542530:CDS 40.0%
! AGGGCTGGTGTAAATGTAAA+GGG - chr4_1:27630329-27630348 Msa0542530:CDS 40.0%
! GGACAATTTTGAATGGCACA+AGG - chr4_1:27630373-27630392 Msa0542530:CDS 40.0%
ATCAAAAGCTCTCAGGATTG+TGG - chr4_1:27630453-27630472 Msa0542530:CDS 40.0%
CGATGACAAAAACAAGGGTG+CGG + chr4_1:27627772-27627791 None:intergenic 45.0%
AACACTGCACCACTGCAAAT+AGG - chr4_1:27627911-27627930 Msa0542530:CDS 45.0%
! TATGAAGGTGCAGCAAGTGA+AGG - chr4_1:27627998-27628017 Msa0542530:CDS 45.0%
TGGAAGGGGACCAAGTATAT+GGG - chr4_1:27628021-27628040 Msa0542530:CDS 45.0%
! TGGTCTAGAATACTCCCAGA+TGG - chr4_1:27628184-27628203 Msa0542530:CDS 45.0%
! GGTCTAGAATACTCCCAGAT+GGG - chr4_1:27628185-27628204 Msa0542530:CDS 45.0%
CTCCACTTAGTTTCCCATCT+GGG + chr4_1:27628201-27628220 None:intergenic 45.0%
AGCTCGCACAATTTCTACGA+GGG + chr4_1:27628710-27628729 None:intergenic 45.0%
AAGCTCGCACAATTTCTACG+AGG + chr4_1:27628711-27628730 None:intergenic 45.0%
TGCAAACCTGTGACATGAGA+TGG + chr4_1:27628764-27628783 None:intergenic 45.0%
CCATCTTGACTGTATGTCCA+TGG + chr4_1:27629044-27629063 None:intergenic 45.0%
CCATGGACATACAGTCAAGA+TGG - chr4_1:27629041-27629060 Msa0542530:CDS 45.0%
TTGAATCCAAATTGCACCGG+TGG - chr4_1:27629107-27629126 Msa0542530:CDS 45.0%
! GTCTCTCAAAAGGGTGTGAT+AGG - chr4_1:27629218-27629237 Msa0542530:CDS 45.0%
TGAGAGCCTTAGTCAGAACA+AGG - chr4_1:27629483-27629502 Msa0542530:intron 45.0%
TGATCCATCGTTATCGCTTG+AGG - chr4_1:27630143-27630162 Msa0542530:CDS 45.0%
GATTCCTCAAGCGATAACGA+TGG + chr4_1:27630150-27630169 None:intergenic 45.0%
! CAGGGCTGGTGTAAATGTAA+AGG - chr4_1:27630328-27630347 Msa0542530:CDS 45.0%
!! AATAATTATTTATAAAAGTT+CGG + chr4_1:27628555-27628574 None:intergenic 5.0%
TGCAGCAAGTGAAGGTGGAA+GGG - chr4_1:27628006-27628025 Msa0542530:CDS 50.0%
GTGGAAGGGGACCAAGTATA+TGG - chr4_1:27628020-27628039 Msa0542530:CDS 50.0%
CTCCCAGATGGGAAACTAAG+TGG - chr4_1:27628196-27628215 Msa0542530:CDS 50.0%
CCTCCACTTAGTTTCCCATC+TGG + chr4_1:27628202-27628221 None:intergenic 50.0%
CCAGATGGGAAACTAAGTGG+AGG - chr4_1:27628199-27628218 Msa0542530:CDS 50.0%
CTCGCACAATTTCTACGAGG+GGG + chr4_1:27628708-27628727 None:intergenic 50.0%
GCTCGCACAATTTCTACGAG+GGG + chr4_1:27628709-27628728 None:intergenic 50.0%
GCAAATGGTGAAATGGCACC+AGG - chr4_1:27629068-27629087 Msa0542530:CDS 50.0%
!! AACCAAGAAGAGCATCGACC+TGG + chr4_1:27629089-27629108 None:intergenic 50.0%
GAATCACCACCGGTGCAATT+TGG + chr4_1:27629116-27629135 None:intergenic 50.0%
! AATTGCACCGGTGGTGATTC+TGG - chr4_1:27629116-27629135 Msa0542530:CDS 50.0%
TTCGGTTCCAGAATCACCAC+CGG + chr4_1:27629126-27629145 None:intergenic 50.0%
!! ACTTCTAGCTCATGCAGCAG+TGG - chr4_1:27629169-27629188 Msa0542530:CDS 50.0%
GAGCAATCTAGGCTAGTGAG+TGG - chr4_1:27629524-27629543 Msa0542530:CDS 50.0%
GTGATTGCAGTCGAACGTGA+TGG - chr4_1:27629767-27629786 Msa0542530:intron 50.0%
! GAAGGTGCAGCAAGTGAAGG+TGG - chr4_1:27628001-27628020 Msa0542530:CDS 55.0%
GTGCAGCAAGTGAAGGTGGA+AGG - chr4_1:27628005-27628024 Msa0542530:CDS 55.0%
GCAGCAAGTGAAGGTGGAAG+GGG - chr4_1:27628007-27628026 Msa0542530:CDS 55.0%
!! TGCATTTTAGCCAGGGGCGA+AGG - chr4_1:27628314-27628333 Msa0542530:intron 55.0%
!! GCATTTTAGCCAGGGGCGAA+GGG - chr4_1:27628315-27628334 Msa0542530:intron 55.0%
TCATGGCCATGGGGTAGCAA+TGG - chr4_1:27628340-27628359 Msa0542530:intron 55.0%
CACCAGGTCGATGCTCTTCT+TGG - chr4_1:27629084-27629103 Msa0542530:CDS 55.0%
GCGAAGGGCTTCATGGCCAT+GGG - chr4_1:27628330-27628349 Msa0542530:intron 60.0%
CGAAGGGCTTCATGGCCATG+GGG - chr4_1:27628331-27628350 Msa0542530:intron 60.0%
GGGTAGCCATTGCTACCCCA+TGG + chr4_1:27628349-27628368 None:intergenic 60.0%
GGCGAAGGGCTTCATGGCCA+TGG - chr4_1:27628329-27628348 Msa0542530:intron 65.0%
GCCAGGGGCGAAGGGCTTCA+TGG - chr4_1:27628323-27628342 Msa0542530:intron 70.0%
GCCATGAAGCCCTTCGCCCC+TGG + chr4_1:27628327-27628346 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 27627724 27630650 27627724 ID=Msa0542530;Name=Msa0542530
chr4_1 mRNA 27627724 27630650 27627724 ID=Msa0542530-mRNA-1;Parent=Msa0542530;Name=Msa0542530-mRNA-1;_AED=0.10;_eAED=0.10;_QI=106|1|1|1|1|1|8|133|519
chr4_1 exon 27627724 27628043 27627724 ID=Msa0542530-mRNA-1:exon:7514;Parent=Msa0542530-mRNA-1
chr4_1 exon 27628139 27628247 27628139 ID=Msa0542530-mRNA-1:exon:7513;Parent=Msa0542530-mRNA-1
chr4_1 exon 27628362 27628464 27628362 ID=Msa0542530-mRNA-1:exon:7512;Parent=Msa0542530-mRNA-1
chr4_1 exon 27628566 27628597 27628566 ID=Msa0542530-mRNA-1:exon:7511;Parent=Msa0542530-mRNA-1
chr4_1 exon 27628715 27628932 27628715 ID=Msa0542530-mRNA-1:exon:7510;Parent=Msa0542530-mRNA-1
chr4_1 exon 27629041 27629412 27629041 ID=Msa0542530-mRNA-1:exon:7509;Parent=Msa0542530-mRNA-1
chr4_1 exon 27629510 27629597 27629510 ID=Msa0542530-mRNA-1:exon:7508;Parent=Msa0542530-mRNA-1
chr4_1 exon 27630094 27630650 27630094 ID=Msa0542530-mRNA-1:exon:7507;Parent=Msa0542530-mRNA-1
chr4_1 five_prime_UTR 27630545 27630650 27630545 ID=Msa0542530-mRNA-1:five_prime_utr;Parent=Msa0542530-mRNA-1
chr4_1 CDS 27630094 27630544 27630094 ID=Msa0542530-mRNA-1:cds;Parent=Msa0542530-mRNA-1
chr4_1 CDS 27629510 27629597 27629510 ID=Msa0542530-mRNA-1:cds;Parent=Msa0542530-mRNA-1
chr4_1 CDS 27629041 27629412 27629041 ID=Msa0542530-mRNA-1:cds;Parent=Msa0542530-mRNA-1
chr4_1 CDS 27628715 27628932 27628715 ID=Msa0542530-mRNA-1:cds;Parent=Msa0542530-mRNA-1
chr4_1 CDS 27628566 27628597 27628566 ID=Msa0542530-mRNA-1:cds;Parent=Msa0542530-mRNA-1
chr4_1 CDS 27628362 27628464 27628362 ID=Msa0542530-mRNA-1:cds;Parent=Msa0542530-mRNA-1
chr4_1 CDS 27628139 27628247 27628139 ID=Msa0542530-mRNA-1:cds;Parent=Msa0542530-mRNA-1
chr4_1 CDS 27627857 27628043 27627857 ID=Msa0542530-mRNA-1:cds;Parent=Msa0542530-mRNA-1
chr4_1 three_prime_UTR 27627724 27627856 27627724 ID=Msa0542530-mRNA-1:three_prime_utr;Parent=Msa0542530-mRNA-1
Gene Sequence

>Msa0542530

ATGAGATATTCCCTATTCTCTCTCTTGTTGTCTATAGTTTCAACATTCATTATTACTTTAACAGAAGGTCAAGAAATAATTAACACTGCACCACTGCAAATAGGTTCATTGAATAGGAAAGATTTTCCAGAAGGATTCATATTTGGCACAGCATCTTCTGCATATCAATATGAAGGTGCAGCAAGTGAAGGTGGAAGGGGACCAAGTATATGGGATACTTTCACTCATAGATATCCTCAAAAAATCAGTGATGGAAACAATGGAGACGTTGCAGTTGACTCGTATCATCGCTATAAGGAAGATGTTGGAATCATGAAGGATATGAACTTGGATGCATACAGATTTTCTATTTCTTGGTCTAGAATACTCCCAGATGGGAAACTAAGTGGAGGAATTAATCAAGAAGGAATCGACTATTACAACAACCTCATTAATGAATTGGTGGCAAATGGTTTGCAACCATTTGTAACACTTTTTCATTGGGATCTTCCTCAAACTCTAGAAGATGAATATGGCGGTTTCTTAAGCCCTCTCATCATAAAAGATTTCCAAGATTATGCGGAACTTTGCTTTAAAACATTTGGAGATAGAGTTAAACATTGGATAACTTTGAATGAACCATGGACATACAGTCAAGATGGGTATGCAAATGGTGAAATGGCACCAGGTCGATGCTCTTCTTGGTTGAATCCAAATTGCACCGGTGGTGATTCTGGAACCGAACCATATTTAGTTGCACATTATCAACTTCTAGCTCATGCAGCAGTGGTTAATTTGTACAAGACCAAATATCAAGTCTCTCAAAAGGGTGTGATAGGTATAACATTGGTTATTAACTATTTTGTGCCACTCTCAGATAACAAACTTGATATAAAGGCTGCTGAGAGAGCTACAGACTTTATGTTTGGATGGTTTATGGATCCCTTAGCCAATGGAGATTATCCAAAAAACATGAGAGCCTTAGTCAGAACAAGGTTACCAAAGTTTACAAAAGAGCAATCTAGGCTAGTGAGTGGTTCATTTGATTTTATTGGCATAAATTACTACTCTTCATGTTATGCTTCTGATGCACCTCAATTAAGCAATGGCAAACCTAGCTACCTAACAGATTCACTTTCTCGTTTTTCATTCGAACGTGATGGAAAAACCATTGGTCTAAATGTTGCTTCAAATTGGTTGTATGTTTATCCAAGAGCAATTCGAGACTTTCTGATACAAGTTAAAGAAAAATATAACAACCCTTTAATTTATATTACTGAAAATGGTATTAATGAGTACGATGATCCATCGTTATCGCTTGAGGAATCTCTTATGGATACCTATAGAGTTGATTATCATTATCGTCATTTATTCTATCTTAATGAGGCAATCAAGGCTGGTGTAAATGTAAAGGGATACTTTGCATGGTCTTTGTTGGACAATTTTGAATGGCACAAGGGTTATACCGTAAGATTTGGAATGACCTTTGTAGATTACAAAAATGGTTTGAAAAGATATCAAAAGCTCTCAGGATTGTGGTTCAAGAATTTTCTCACATCAGAGAACATAGATTCCATTTAA
Protein sequence

>Msa0542530

MRYSLFSLLLSIVSTFIITLTEGQEIINTAPLQIGSLNRKDFPEGFIFGTASSAYQYEGAASEGGRGPSIWDTFTHRYPQKISDGNNGDVAVDSYHRYKEDVGIMKDMNLDAYRFSISWSRILPDGKLSGGINQEGIDYYNNLINELVANGLQPFVTLFHWDLPQTLEDEYGGFLSPLIIKDFQDYAELCFKTFGDRVKHWITLNEPWTYSQDGYANGEMAPGRCSSWLNPNCTGGDSGTEPYLVAHYQLLAHAAVVNLYKTKYQVSQKGVIGITLVINYFVPLSDNKLDIKAAERATDFMFGWFMDPLANGDYPKNMRALVRTRLPKFTKEQSRLVSGSFDFIGINYYSSCYASDAPQLSNGKPSYLTDSLSRFSFERDGKTIGLNVASNWLYVYPRAIRDFLIQVKEKYNNPLIYITENGINEYDDPSLSLEESLMDTYRVDYHYRHLFYLNEAIKAGVNVKGYFAWSLLDNFEWHKGYTVRFGMTFVDYKNGLKRYQKLSGLWFKNFLTSENIDSI*