AlfalfaGEDB | Msa0209860

M. sativa cultivar zhongmu-4 / Msa0209860

KEGG: K17943
GO: GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113
PFAM: PF00806.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209860	XP_003594978.1	83.333	84	8	2	1	84	874	951	4.40e-35	137

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209860	sp\|Q9LJX4\|PUM5_ARATH	58.667	75	26	1	1	75	846	915	5.06e-22	91.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209860	G7IN26	83.333	84	8	2	1	84	874	951	2.10e-35	137

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0021510	Msa0209860	0.800293	1.542838e-48	-8.615850e-47
Msa0031710	Msa0209860	0.821064	4.924496e-53	-8.615850e-47
Msa0043980	Msa0209860	0.803909	2.784026e-49	-8.615850e-47
Msa0074700	Msa0209860	0.825337	4.952464e-54	-8.615850e-47
Msa0086760	Msa0209860	0.803312	3.702305e-49	-8.615850e-47
Msa0114060	Msa0209860	0.820480	6.709158e-53	-8.615850e-47
Msa0209860	Msa0220580	0.802384	5.756790e-49	-8.615850e-47
Msa0209860	Msa0257460	0.801463	8.898865e-49	-8.615850e-47
Msa0209860	Msa0260680	0.801563	8.487429e-49	-8.615850e-47
Msa0209860	Msa0280090	0.830530	2.788567e-55	-8.615850e-47
Msa0209860	Msa0309770	0.801482	8.819683e-49	-8.615850e-47
Msa0209860	Msa0397750	0.803113	4.069933e-49	-8.615850e-47
Msa0209860	Msa0548240	0.805221	1.482105e-49	-8.615850e-47
Msa0209860	Msa0607050	0.802332	5.899640e-49	-8.615850e-47
Msa0209860	Msa0610430	0.803505	3.376024e-49	-8.615850e-47
Msa0209860	Msa0681270	0.808590	2.873655e-50	-8.615850e-47
Msa0209860	Msa0709400	0.823988	1.029816e-53	-8.615850e-47
Msa0209860	Msa0802010	0.810583	1.072264e-50	-8.615850e-47
Msa0209860	Msa0880020	0.802927	4.447704e-49	-8.615850e-47
Msa0209860	Msa0945970	0.812162	4.870939e-51	-8.615850e-47
Msa0209860	Msa0947660	0.804889	1.739435e-49	-8.615850e-47
Msa0209860	Msa0969810	0.806364	8.528632e-50	-8.615850e-47
Msa0209860	Msa1035480	0.802799	4.725058e-49	-8.615850e-47
Msa0209860	Msa1075650	0.804294	2.314736e-49	-8.615850e-47
Msa0209860	Msa1081980	0.816147	6.425511e-52	-8.615850e-47
Msa0209860	Msa1109680	0.801423	9.069323e-49	-8.615850e-47
Msa0209860	Msa1124960	0.814333	1.625330e-51	-8.615850e-47
Msa0209860	Msa1174040	0.821838	3.262986e-53	-8.615850e-47
Msa0209860	Msa1204090	0.801446	8.969128e-49	-8.615850e-47
Msa0209860	Msa1225130	0.801645	8.167239e-49	-8.615850e-47
Msa0209860	Msa1228590	0.810355	1.201437e-50	-8.615850e-47
Msa0209860	Msa1452240	0.838127	3.457773e-57	-8.615850e-47
Msa0209860	Msa1460830	0.804457	2.141001e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209860	MtrunA17_Chr2g0298151	83.333	84	8	2	1	84	874	951	4.05e-39	137
Msa0209860	MtrunA17_Chr5g0435601	50.667	75	32	1	1	75	890	959	3.13e-20	83.2
Msa0209860	MtrunA17_Chr2g0328561	52.000	75	31	1	1	75	930	999	3.53e-20	83.2
Msa0209860	MtrunA17_Chr4g0031951	50.000	74	32	1	1	74	908	976	1.12e-19	81.6
Msa0209860	MtrunA17_Chr1g0199451	46.053	76	36	1	1	76	860	930	3.80e-18	77.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209860	AT3G20250.3	58.667	75	26	1	1	75	834	903	5.67e-23	91.7
Msa0209860	AT3G20250.2	58.667	75	26	1	1	75	834	903	5.67e-23	91.7
Msa0209860	AT3G20250.1	58.667	75	26	1	1	75	798	867	6.61e-23	91.3
Msa0209860	AT4G25880.3	57.534	73	26	1	1	73	744	811	7.27e-23	91.3
Msa0209860	AT4G25880.4	57.534	73	26	1	1	73	750	817	8.41e-23	90.9
Msa0209860	AT4G25880.2	57.534	73	26	1	1	73	744	811	8.74e-23	90.9
Msa0209860	AT4G25880.1	57.534	73	26	1	1	73	750	817	9.73e-23	90.9
Msa0209860	AT4G25880.5	57.534	73	26	1	1	73	750	817	9.73e-23	90.9
Msa0209860	AT2G29140.2	52.000	75	31	1	1	75	844	913	1.16e-20	84.7
Msa0209860	AT2G29140.1	52.000	75	31	1	1	75	844	913	1.16e-20	84.7
Msa0209860	AT2G29200.1	52.000	75	31	1	1	75	848	917	1.89e-20	84.3
Msa0209860	AT2G29200.2	52.000	75	31	1	1	75	848	917	1.89e-20	84.3
Msa0209860	AT2G29190.2	52.000	75	31	1	1	75	852	921	5.10e-20	83.2
Msa0209860	AT2G29190.1	52.000	75	31	1	1	75	852	921	5.10e-20	83.2
Msa0209860	AT3G10360.1	48.684	76	34	1	1	76	882	952	1.24e-18	79.0

Find 15 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAGCAAAATGATAATTTAT+TGG	0.212561	2_1:-49353413	Msa0209860:intron
AACAGATATCAATAACCTTC+TGG	0.308312	2_1:+49352420	None:intergenic
GCTTTGAAGAAATACACTTA+TGG	0.333397	2_1:-49352346	Msa0209860:three_prime_UTR
GTATGGTGACGCCTCTGAAC+GGG	0.439564	2_1:-49353482	Msa0209860:CDS
GTGGAGAAATGTCTGGAGTA+TGG	0.492945	2_1:-49353499	Msa0209860:CDS
CAAATTATCAGCAAGCTGTC+AGG	0.494856	2_1:-49353565	None:intergenic
GAATGTTGTGGAGAAATGTC+TGG	0.499195	2_1:-49353506	Msa0209860:CDS
CATTCGAAGCAAACTTATGC+TGG	0.502819	2_1:+49353524	None:intergenic
AAAACACATTGTGGCTCGTA+TGG	0.505806	2_1:-49352323	Msa0209860:three_prime_UTR
AATTAACACTTCCCGTTCAG+AGG	0.538629	2_1:+49353471	None:intergenic
TAAGTTTGCTTCGAATGTTG+TGG	0.567052	2_1:-49353518	Msa0209860:CDS
TGCGAATTATGTGATCCAGA+AGG	0.601603	2_1:-49352435	Msa0209860:CDS
AGTATGGTGACGCCTCTGAA+CGG	0.619509	2_1:-49353483	Msa0209860:CDS
CACTTATGGAAAACACATTG+TGG	0.626540	2_1:-49352332	Msa0209860:three_prime_UTR
TGAACGGGAAGTGTTAATTG+CGG	0.722208	2_1:-49353467	Msa0209860:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTCTTTTCAATAAATTA+TGG	-	chr2_1:49353540-49353559	Msa0209860:CDS	10.0%
!!!	AATTTCTTTTCAATAAATTA+TGG	-	chr2_1:49353540-49353559	Msa0209860:CDS	10.0%
!!!	ATGTCTTTAGTAATTTTTTA+TGG	-	chr2_1:49352569-49352588	Msa0209860:intron	15.0%
!!	AAAACAAAACAAAAATTACA+GGG	+	chr2_1:49352633-49352652	None:intergenic	15.0%
!!	AAAAACAAAACAAAAATTAC+AGG	+	chr2_1:49352634-49352653	None:intergenic	15.0%
!!	ATATGAAGTGTAAAAATAAT+GGG	+	chr2_1:49352844-49352863	None:intergenic	15.0%
!!	TTCAGTTACAATAATTTAAT+TGG	-	chr2_1:49352878-49352897	Msa0209860:intron	15.0%
!!!	ATGTCTTTAGTAATTTTTTA+TGG	-	chr2_1:49352569-49352588	Msa0209860:intron	15.0%
!!	AAAACAAAACAAAAATTACA+GGG	+	chr2_1:49352633-49352652	None:intergenic	15.0%
!!	AAAAACAAAACAAAAATTAC+AGG	+	chr2_1:49352634-49352653	None:intergenic	15.0%
!!	ATATGAAGTGTAAAAATAAT+GGG	+	chr2_1:49352844-49352863	None:intergenic	15.0%
!!	TTCAGTTACAATAATTTAAT+TGG	-	chr2_1:49352878-49352897	Msa0209860:intron	15.0%
!!	TGAGCAAAATGATAATTTAT+TGG	-	chr2_1:49352413-49352432	Msa0209860:CDS	20.0%
!!!	TGGAAAGTTCTTTTATTTTA+AGG	+	chr2_1:49352493-49352512	None:intergenic	20.0%
!!	GATATGAAGTGTAAAAATAA+TGG	+	chr2_1:49352845-49352864	None:intergenic	20.0%
!!	TTATTATTATTGACCGAAAA+TGG	-	chr2_1:49353112-49353131	Msa0209860:intron	20.0%
!!!	ATCTGAATTTTTACTAGTAT+TGG	-	chr2_1:49353227-49353246	Msa0209860:intron	20.0%
!!!	TAATTGGAAGATTTTATAAC+TGG	-	chr2_1:49353282-49353301	Msa0209860:intron	20.0%
!!	TCTGCAATGAAAATAAAAAT+AGG	+	chr2_1:49353352-49353371	None:intergenic	20.0%
!!	TGAGCAAAATGATAATTTAT+TGG	-	chr2_1:49352413-49352432	Msa0209860:CDS	20.0%
!!!	TGGAAAGTTCTTTTATTTTA+AGG	+	chr2_1:49352493-49352512	None:intergenic	20.0%
!!	GATATGAAGTGTAAAAATAA+TGG	+	chr2_1:49352845-49352864	None:intergenic	20.0%
!!	TTATTATTATTGACCGAAAA+TGG	-	chr2_1:49353112-49353131	Msa0209860:intron	20.0%
!!!	ATCTGAATTTTTACTAGTAT+TGG	-	chr2_1:49353227-49353246	Msa0209860:intron	20.0%
!!!	TAATTGGAAGATTTTATAAC+TGG	-	chr2_1:49353282-49353301	Msa0209860:intron	20.0%
!!	TCTGCAATGAAAATAAAAAT+AGG	+	chr2_1:49353352-49353371	None:intergenic	20.0%
!!	TTTCCATTCATTGTCTTATT+TGG	-	chr2_1:49352507-49352526	Msa0209860:intron	25.0%
!!!	AATATGTCACTTTTTGCATT+TGG	-	chr2_1:49352664-49352683	Msa0209860:intron	25.0%
!	AAATATGCATCAAAACAACT+AGG	+	chr2_1:49352691-49352710	None:intergenic	25.0%
!!!	TTGTTTTGATGCATATTTTC+CGG	-	chr2_1:49352693-49352712	Msa0209860:intron	25.0%
!	TTTAAATATGTTTCCATTCC+CGG	+	chr2_1:49352715-49352734	None:intergenic	25.0%
!	TCATTATGATGAAGACATTA+TGG	-	chr2_1:49352769-49352788	Msa0209860:intron	25.0%
!	CATTATGATGAAGACATTAT+GGG	-	chr2_1:49352770-49352789	Msa0209860:intron	25.0%
!	GTTACAATAATTTAATTGGC+TGG	-	chr2_1:49352882-49352901	Msa0209860:intron	25.0%
!	GTGAATAATGCTTAACTAAT+TGG	-	chr2_1:49353266-49353285	Msa0209860:intron	25.0%
!!	TTTCCATTCATTGTCTTATT+TGG	-	chr2_1:49352507-49352526	Msa0209860:intron	25.0%
!!!	AATATGTCACTTTTTGCATT+TGG	-	chr2_1:49352664-49352683	Msa0209860:intron	25.0%
!	AAATATGCATCAAAACAACT+AGG	+	chr2_1:49352691-49352710	None:intergenic	25.0%
!!!	TTGTTTTGATGCATATTTTC+CGG	-	chr2_1:49352693-49352712	Msa0209860:intron	25.0%
!	TTTAAATATGTTTCCATTCC+CGG	+	chr2_1:49352715-49352734	None:intergenic	25.0%
!	TCATTATGATGAAGACATTA+TGG	-	chr2_1:49352769-49352788	Msa0209860:intron	25.0%
!	CATTATGATGAAGACATTAT+GGG	-	chr2_1:49352770-49352789	Msa0209860:intron	25.0%
!	GTTACAATAATTTAATTGGC+TGG	-	chr2_1:49352882-49352901	Msa0209860:intron	25.0%
!	GTGAATAATGCTTAACTAAT+TGG	-	chr2_1:49353266-49353285	Msa0209860:intron	25.0%
	TCACCAAATAAGACAATGAA+TGG	+	chr2_1:49352513-49352532	None:intergenic	30.0%
	CAAAAAGTGACATATTAGCA+GGG	+	chr2_1:49352660-49352679	None:intergenic	30.0%
!!!	TGTTTTGATGCATATTTTCC+GGG	-	chr2_1:49352694-49352713	Msa0209860:intron	30.0%
	TAAAACAAGCTTCTTTCCTA+GGG	+	chr2_1:49352981-49353000	None:intergenic	30.0%
	ATAAAACAAGCTTCTTTCCT+AGG	+	chr2_1:49352982-49353001	None:intergenic	30.0%
	AATTTCCAGCTTGAATTTCT+GGG	+	chr2_1:49353088-49353107	None:intergenic	30.0%
	TAACAGAATGCAAAAGATAG+TGG	-	chr2_1:49353161-49353180	Msa0209860:intron	30.0%
	TGGAATGTAAGGATACTTTA+AGG	-	chr2_1:49353181-49353200	Msa0209860:intron	30.0%
	GGAATGTAAGGATACTTTAA+GGG	-	chr2_1:49353182-49353201	Msa0209860:intron	30.0%
	AACAGATATCAATAACCTTC+TGG	+	chr2_1:49353409-49353428	None:intergenic	30.0%
!!	GCTTTGAAGAAATACACTTA+TGG	-	chr2_1:49353480-49353499	Msa0209860:CDS	30.0%
	TCACCAAATAAGACAATGAA+TGG	+	chr2_1:49352513-49352532	None:intergenic	30.0%
	CAAAAAGTGACATATTAGCA+GGG	+	chr2_1:49352660-49352679	None:intergenic	30.0%
!!!	TGTTTTGATGCATATTTTCC+GGG	-	chr2_1:49352694-49352713	Msa0209860:intron	30.0%
	TAAAACAAGCTTCTTTCCTA+GGG	+	chr2_1:49352981-49353000	None:intergenic	30.0%
	ATAAAACAAGCTTCTTTCCT+AGG	+	chr2_1:49352982-49353001	None:intergenic	30.0%
	AATTTCCAGCTTGAATTTCT+GGG	+	chr2_1:49353088-49353107	None:intergenic	30.0%
	TAACAGAATGCAAAAGATAG+TGG	-	chr2_1:49353161-49353180	Msa0209860:intron	30.0%
	TGGAATGTAAGGATACTTTA+AGG	-	chr2_1:49353181-49353200	Msa0209860:intron	30.0%
	GGAATGTAAGGATACTTTAA+GGG	-	chr2_1:49353182-49353201	Msa0209860:intron	30.0%
	AACAGATATCAATAACCTTC+TGG	+	chr2_1:49353409-49353428	None:intergenic	30.0%
!!	GCTTTGAAGAAATACACTTA+TGG	-	chr2_1:49353480-49353499	Msa0209860:CDS	30.0%
!	TAAGTTTGCTTCGAATGTTG+TGG	-	chr2_1:49352308-49352327	Msa0209860:three_prime_UTR	35.0%
	GCAAAAAGTGACATATTAGC+AGG	+	chr2_1:49352661-49352680	None:intergenic	35.0%
!!	TCAGAATAGTGGATTCTCAT+TGG	-	chr2_1:49352909-49352928	Msa0209860:intron	35.0%
	ATTTATCCCCTTTAAACCCT+AGG	-	chr2_1:49352962-49352981	Msa0209860:intron	35.0%
	TTCTTTCCTAGGGTTTAAAG+GGG	+	chr2_1:49352971-49352990	None:intergenic	35.0%
	CTTCTTTCCTAGGGTTTAAA+GGG	+	chr2_1:49352972-49352991	None:intergenic	35.0%
	CAATTTCCAGCTTGAATTTC+TGG	+	chr2_1:49353089-49353108	None:intergenic	35.0%
!!	GCAAAAGATAGTGGAATGTA+AGG	-	chr2_1:49353170-49353189	Msa0209860:intron	35.0%
	GATGTCTCGAACATCAAAAT+TGG	+	chr2_1:49353210-49353229	None:intergenic	35.0%
	CACTTATGGAAAACACATTG+TGG	-	chr2_1:49353494-49353513	Msa0209860:CDS	35.0%
!	TAAGTTTGCTTCGAATGTTG+TGG	-	chr2_1:49352308-49352327	Msa0209860:three_prime_UTR	35.0%
	GCAAAAAGTGACATATTAGC+AGG	+	chr2_1:49352661-49352680	None:intergenic	35.0%
!!	TCAGAATAGTGGATTCTCAT+TGG	-	chr2_1:49352909-49352928	Msa0209860:intron	35.0%
	ATTTATCCCCTTTAAACCCT+AGG	-	chr2_1:49352962-49352981	Msa0209860:intron	35.0%
	TTCTTTCCTAGGGTTTAAAG+GGG	+	chr2_1:49352971-49352990	None:intergenic	35.0%
	CTTCTTTCCTAGGGTTTAAA+GGG	+	chr2_1:49352972-49352991	None:intergenic	35.0%
	CAATTTCCAGCTTGAATTTC+TGG	+	chr2_1:49353089-49353108	None:intergenic	35.0%
!!	GCAAAAGATAGTGGAATGTA+AGG	-	chr2_1:49353170-49353189	Msa0209860:intron	35.0%
	GATGTCTCGAACATCAAAAT+TGG	+	chr2_1:49353210-49353229	None:intergenic	35.0%
	CACTTATGGAAAACACATTG+TGG	-	chr2_1:49353494-49353513	Msa0209860:CDS	35.0%
	CATTCGAAGCAAACTTATGC+TGG	+	chr2_1:49352305-49352324	None:intergenic	40.0%
	GAATGTTGTGGAGAAATGTC+TGG	-	chr2_1:49352320-49352339	Msa0209860:three_prime_UTR	40.0%
	AATTAACACTTCCCGTTCAG+AGG	+	chr2_1:49352358-49352377	None:intergenic	40.0%
!!	TGAACGGGAAGTGTTAATTG+CGG	-	chr2_1:49352359-49352378	Msa0209860:exon	40.0%
!	TGATGCATATTTTCCGGGAA+TGG	-	chr2_1:49352699-49352718	Msa0209860:intron	40.0%
	CATTATGGGACATGCAATGT+CGG	-	chr2_1:49352784-49352803	Msa0209860:intron	40.0%
	GCTTCTTTCCTAGGGTTTAA+AGG	+	chr2_1:49352973-49352992	None:intergenic	40.0%
	ACAGTTAACAGACGTTCATG+TGG	+	chr2_1:49353023-49353042	None:intergenic	40.0%
!	GAGTCGTTGAACTCCATTTT+CGG	+	chr2_1:49353128-49353147	None:intergenic	40.0%
!!	GGCGTGAATGTATGTCATTT+TGG	-	chr2_1:49353303-49353322	Msa0209860:intron	40.0%
	TGCGAATTATGTGATCCAGA+AGG	-	chr2_1:49353391-49353410	Msa0209860:intron	40.0%
!	TTCTGAAAAATCAGCGAGCA+AGG	-	chr2_1:49353427-49353446	Msa0209860:CDS	40.0%
	AAAACACATTGTGGCTCGTA+TGG	-	chr2_1:49353503-49353522	Msa0209860:CDS	40.0%
	ATTGAACAACATGTCCTGAC+AGG	+	chr2_1:49352278-49352297	None:intergenic	40.0%
	CATTCGAAGCAAACTTATGC+TGG	+	chr2_1:49352305-49352324	None:intergenic	40.0%
	GAATGTTGTGGAGAAATGTC+TGG	-	chr2_1:49352320-49352339	Msa0209860:three_prime_UTR	40.0%
	AATTAACACTTCCCGTTCAG+AGG	+	chr2_1:49352358-49352377	None:intergenic	40.0%
!!	TGAACGGGAAGTGTTAATTG+CGG	-	chr2_1:49352359-49352378	Msa0209860:exon	40.0%
!	TGATGCATATTTTCCGGGAA+TGG	-	chr2_1:49352699-49352718	Msa0209860:intron	40.0%
	CATTATGGGACATGCAATGT+CGG	-	chr2_1:49352784-49352803	Msa0209860:intron	40.0%
	GCTTCTTTCCTAGGGTTTAA+AGG	+	chr2_1:49352973-49352992	None:intergenic	40.0%
	ACAGTTAACAGACGTTCATG+TGG	+	chr2_1:49353023-49353042	None:intergenic	40.0%
!	GAGTCGTTGAACTCCATTTT+CGG	+	chr2_1:49353128-49353147	None:intergenic	40.0%
!!	GGCGTGAATGTATGTCATTT+TGG	-	chr2_1:49353303-49353322	Msa0209860:intron	40.0%
	TGCGAATTATGTGATCCAGA+AGG	-	chr2_1:49353391-49353410	Msa0209860:intron	40.0%
!	TTCTGAAAAATCAGCGAGCA+AGG	-	chr2_1:49353427-49353446	Msa0209860:CDS	40.0%
	AAAACACATTGTGGCTCGTA+TGG	-	chr2_1:49353503-49353522	Msa0209860:CDS	40.0%
!	GTGGAGAAATGTCTGGAGTA+TGG	-	chr2_1:49352327-49352346	Msa0209860:three_prime_UTR	45.0%
	TGGCTGGTATGTCAGAATAG+TGG	-	chr2_1:49352898-49352917	Msa0209860:intron	45.0%
	TATGTGTCATCCCTAGTCAG+AGG	-	chr2_1:49353049-49353068	Msa0209860:intron	45.0%
	TATCGCAATGCCTCTGACTA+GGG	+	chr2_1:49353062-49353081	None:intergenic	45.0%
	TAGGACCCAGAAATTCAAGC+TGG	-	chr2_1:49353080-49353099	Msa0209860:intron	45.0%
!	GTGGAGAAATGTCTGGAGTA+TGG	-	chr2_1:49352327-49352346	Msa0209860:three_prime_UTR	45.0%
	TGGCTGGTATGTCAGAATAG+TGG	-	chr2_1:49352898-49352917	Msa0209860:intron	45.0%
	TATGTGTCATCCCTAGTCAG+AGG	-	chr2_1:49353049-49353068	Msa0209860:intron	45.0%
	TATCGCAATGCCTCTGACTA+GGG	+	chr2_1:49353062-49353081	None:intergenic	45.0%
	TAGGACCCAGAAATTCAAGC+TGG	-	chr2_1:49353080-49353099	Msa0209860:intron	45.0%
!	AGTATGGTGACGCCTCTGAA+CGG	-	chr2_1:49352343-49352362	Msa0209860:three_prime_UTR	50.0%
	CTATCGCAATGCCTCTGACT+AGG	+	chr2_1:49353063-49353082	None:intergenic	50.0%
!!	CTAGTCAGAGGCATTGCGAT+AGG	-	chr2_1:49353061-49353080	Msa0209860:intron	50.0%
!	AGTATGGTGACGCCTCTGAA+CGG	-	chr2_1:49352343-49352362	Msa0209860:three_prime_UTR	50.0%
	CTATCGCAATGCCTCTGACT+AGG	+	chr2_1:49353063-49353082	None:intergenic	50.0%
!!	CTAGTCAGAGGCATTGCGAT+AGG	-	chr2_1:49353061-49353080	Msa0209860:intron	50.0%
!	GTATGGTGACGCCTCTGAAC+GGG	-	chr2_1:49352344-49352363	Msa0209860:three_prime_UTR	55.0%
!	GTATGGTGACGCCTCTGAAC+GGG	-	chr2_1:49352344-49352363	Msa0209860:three_prime_UTR	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	49352276	49353572	49352276	ID=Msa0209860;Name=Msa0209860
chr2_1	mRNA	49352276	49353572	49352276	ID=Msa0209860-mRNA-1;Parent=Msa0209860;Name=Msa0209860-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|1\|0.5\|1\|1\|1\|2\|94\|88
chr2_1	exon	49352276	49352477	49352276	ID=Msa0209860-mRNA-1:exon:13663;Parent=Msa0209860-mRNA-1
chr2_1	exon	49353414	49353572	49353414	ID=Msa0209860-mRNA-1:exon:13662;Parent=Msa0209860-mRNA-1
chr2_1	CDS	49353414	49353572	49353414	ID=Msa0209860-mRNA-1:cds;Parent=Msa0209860-mRNA-1
chr2_1	CDS	49352370	49352477	49352370	ID=Msa0209860-mRNA-1:cds;Parent=Msa0209860-mRNA-1
chr2_1	three_prime_UTR	49352276	49352369	49352276	ID=Msa0209860-mRNA-1:three_prime_utr;Parent=Msa0209860-mRNA-1

Gene Sequence	>Msa0209860 CTGTCAGGACATGTTGTTCAATTAAGCCAGCATAAGTTTGCTTCGAATGTTGTGGAGAAATGTCTGGAGTATGGTGACGCCTCTGAACGGGAAGTGTTAATTGCGGAGATTATTGCGCATGATGAGCAAATGCATGATGAGCAAAATGATAATTTATTGACAATGATGAAAGATCAATTTGCGAATTATGTGATCCAGAAGGTTATTGATATCTGTTCTGAAAAATCAGCGAGCAAGGTTACTTTCTCACATAAGAGCTCATGCTAA
Protein sequence	>Msa0209860 LSGHVVQLSQHKFASNVVEKCLEYGDASEREVLIAEIIAHDEQMHDEQNDNLLTMMKDQFANYVIQKVIDICSEKSASKVTFSHKSSC*