AlfalfaGEDB | Msa0221550

M. sativa cultivar zhongmu-4 / Msa0221550

KEGG: K10352
GO: -
PFAM: PF07899.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0221550	AES67383.2	88.542	864	63	4	1	833	1	859	0.0	1435

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0221550	G7IU33	88.542	864	63	4	1	833	1	859	0.0	1435

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0032920	Msa0221550	-0.802938	4.423083e-49	-8.615850e-47
Msa0081520	Msa0221550	0.806511	7.941056e-50	-8.615850e-47
Msa0093850	Msa0221550	0.804556	2.041583e-49	-8.615850e-47
Msa0136650	Msa0221550	0.822682	2.078625e-53	-8.615850e-47
Msa0173890	Msa0221550	0.807388	5.180619e-50	-8.615850e-47
Msa0206840	Msa0221550	0.800822	1.203441e-48	-8.615850e-47
Msa0221550	Msa0263080	0.914875	1.145733e-84	-8.615850e-47
Msa0221550	Msa0264870	0.813849	2.078339e-51	-8.615850e-47
Msa0221550	Msa0292400	0.815378	9.531913e-52	-8.615850e-47
Msa0221550	Msa0300320	0.801475	8.848364e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0221550	MtrunA17_Chr2g0325861	88.542	864	63	4	1	833	2	860	0.0	1435
Msa0221550	MtrunA17_Chr2g0325321	51.411	815	293	10	28	795	3	761	0.0	609
Msa0221550	MtrunA17_Chr2g0325231	47.797	908	351	14	4	812	6	889	0.0	602
Msa0221550	MtrunA17_Chr2g0325711	45.377	822	316	13	35	814	26	756	0.0	542
Msa0221550	MtrunA17_Chr2g0325721	62.549	510	163	6	1	491	1	501	1.98e-168	497
Msa0221550	MtrunA17_Chr2g0325721	35.623	393	193	14	281	629	125	501	2.92e-24	107
Msa0221550	MtrunA17_Chr2g0325661	43.687	895	323	17	1	814	1	795	3.12e-158	484
Msa0221550	MtrunA17_Chr2g0325281	53.300	606	207	8	28	587	3	578	1.23e-157	473
Msa0221550	MtrunA17_Chr2g0325281	43.600	250	135	3	393	637	242	490	4.75e-34	139
Msa0221550	MtrunA17_Chr2g0325281	35.227	264	142	5	196	445	353	601	2.51e-16	83.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0221550	AT5G27220.4	25.932	644	405	17	171	791	208	802	2.19e-16	84.7
Msa0221550	AT5G27220.2	25.932	644	405	17	171	791	208	802	2.19e-16	84.7
Msa0221550	AT5G27220.1	26.138	681	407	20	134	791	195	802	3.64e-16	84.0
Msa0221550	AT5G27220.3	26.138	681	407	20	134	791	195	802	3.64e-16	84.0

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Find 160 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCAAAATACAAGAAA+AGG	0.451647	2_1:-70176937	None:intergenic
AAAGAAGCAACTTGAAGGTC+AGG	0.440427	2_1:+70173967	Msa0221550:CDS
AACGTGCAGGTGCATGAAGA+TGG	0.473081	2_1:-70176736	None:intergenic
AAGAGAAGCAAGTCGAAGGA+CGG	0.580246	2_1:+70174134	Msa0221550:CDS
AAGAGAAGCAATGTGATGAA+CGG	0.614667	2_1:+70174008	Msa0221550:CDS
AAGAGAAGCAATTTGATAGC+CGG	0.511794	2_1:+70174797	Msa0221550:CDS
AAGAGAAGCAGTTTGATAGC+CGG	0.545775	2_1:+70174587	Msa0221550:CDS
AAGATAGCTGTAGAGCTGTT+TGG	0.433585	2_1:+70175955	Msa0221550:CDS
AAGGAAGGAAGAGGAACTCA+AGG	0.628919	2_1:+70173663	Msa0221550:CDS
AAGTGAAAATACTGAAAATT+CGG	0.388056	2_1:+70175831	Msa0221550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGTTAGAAAGCTCATTTG+AGG	+	chr2_1:70173309-70173328	Msa0221550:CDS	30.0%
GATAGAACTTAATCAAGTCA+AGG	+	chr2_1:70173579-70173598	Msa0221550:CDS	30.0%
CAGAAAAATTAGAGTCCAAA+AGG	+	chr2_1:70173876-70173895	Msa0221550:CDS	30.0%
TCAATAAAGAAGCAACTTGA+AGG	+	chr2_1:70173962-70173981	Msa0221550:CDS	30.0%
GAAGGAGTTTAAATCAAAAG+AGG	+	chr2_1:70174201-70174220	Msa0221550:CDS	30.0%
TTGATTCAAACTCCTTTACT+TGG	-	chr2_1:70174324-70174343	None:intergenic	30.0%
TCAAAAGAAAGAGAGTTTGA+AGG	+	chr2_1:70174457-70174476	Msa0221550:CDS	30.0%
TTGAATTTAAAGATCGAGTG+AGG	+	chr2_1:70174845-70174864	Msa0221550:CDS	30.0%
TGAATTTAAAGATCGAGTGA+GGG	+	chr2_1:70174846-70174865	Msa0221550:CDS	30.0%
TCAATGCAGAATGAATTTGA+TGG	+	chr2_1:70174913-70174932	Msa0221550:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	70172968	70176993	70172968	ID=Msa0221550;Name=Msa0221550
chr2_1	mRNA	70172968	70176993	70172968	ID=Msa0221550-mRNA-1;Parent=Msa0221550;Name=Msa0221550-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0.33\|0.25\|1\|1\|1\|4\|305\|833
chr2_1	exon	70172968	70173684	70172968	ID=Msa0221550-mRNA-1:exon:19993;Parent=Msa0221550-mRNA-1
chr2_1	exon	70173827	70175012	70173827	ID=Msa0221550-mRNA-1:exon:19994;Parent=Msa0221550-mRNA-1
chr2_1	exon	70175533	70176006	70175533	ID=Msa0221550-mRNA-1:exon:19995;Parent=Msa0221550-mRNA-1
chr2_1	exon	70176564	70176993	70176564	ID=Msa0221550-mRNA-1:exon:19996;Parent=Msa0221550-mRNA-1
chr2_1	CDS	70172968	70173684	70172968	ID=Msa0221550-mRNA-1:cds;Parent=Msa0221550-mRNA-1
chr2_1	CDS	70173827	70175012	70173827	ID=Msa0221550-mRNA-1:cds;Parent=Msa0221550-mRNA-1
chr2_1	CDS	70175533	70176006	70175533	ID=Msa0221550-mRNA-1:cds;Parent=Msa0221550-mRNA-1
chr2_1	CDS	70176564	70176688	70176564	ID=Msa0221550-mRNA-1:cds;Parent=Msa0221550-mRNA-1
chr2_1	three_prime_UTR	70176689	70176993	70176689	ID=Msa0221550-mRNA-1:three_prime_utr;Parent=Msa0221550-mRNA-1

Gene Sequence

>Msa0221550

ATGAATTTTGAGGCTTTTGATAAGCCAAAGTTTTGCGGTAATGGTGATGATGATGGGAGGGATCATGTTTCTTGTGAATACTTTGAATCTGAAGATGATGATGTTCCTTTGTTACCGAGGTTTACCCGGATTTCGAGTTTGGTAAAACGTGATGTTTCTGTTAAGAAGAGGGATTTTGAGTTTGATGACAACAATTCCTTGCCTATTTCTGCTAAGAAATCAAAGGCGTCACTATATCATGATCACGACAAAGATGAAGATGATATCCCGTTGTTAGATTTAATTAAAAGGCCAAGCAAATCAACTGATAAATCATTTTCCTCGCTGAAGAAAGGCCTTGCATTGTTAGAAAGCTCATTTGAGGAAGGCAATAAGAAGACGGAATTAGAAGAGAATAGATTGCAGTCCATAAAGCGAGATATCGAGGAGTGTCGCAAAGAGCTTGAAAATAAGAAAAAGGAAATTAGTTGTTTTAGAGGAATTGACGAAGCTCATGAGAAATTGCAGGGAAAAATTGAAGAATGTGTTAAGGATTTTGTAGTGAAGGAAGGGCAGCTCAATTTGATGGATGACTTGATTGGAGAGCGCAAGCTAGAGCTCAAGACAAAAGAGATAGAACTTAATCAAGTCAAGGGCAACATTTCAAAAGAAATAGAACTTCGTCAAGTCATTGATAATATTGATAAGGACCGTGAAAGGAAGGAAGAGGAACTCAAGGCCAAAGAGAGAGAGCTTGATGCAATGAACAAATTAATTAGTGGGAAAGCAGAAAAATTAGAGTCCAAAAGGAAGAAGTTGCTAAAGCTAACATCAGAAAAGGAGAATGTTCGTGCTCAAATAAAGGAATTTGAGTCAATAAAGAAGCAACTTGAAGGTCAGGTCAAGGAACTTGAGTCAAAAGAGAAGCAATGTGATGAACGGCTGGAGGTGTTGATATCAAGAGAGAAGCAACTTGAAGGACACATGAAGGAGTTTGAATCTAAGATGCAGGAACTTGAAGGTCGTACGATGCAGCTTGAATCAAAAGAGAAGCAAGTCGAAGGACGGGCGATGGAGCTAAAGTTAAAAGAAATGCAACTTAAAGTCCAGGAGAAGGAGTTTAAATCAAAAGAGGAGAAGTTTGAAGGCCTAATGAAGGAGGTGGAGTTTAAAAAGGAGCACTTTGAAAACCAATCGAATGTGTTAGAGTCAATAGATAACCAACTTGTATGCCAAGTAAAGGAGTTTGAATCAAAGCAGAAGGAATTTGAATGCCAAAAGAAGGAGTCGATAGTAAGACAAAAATATTTCAAAAGCCGGCTCGAGTCAGGAGAGAAGAAACATGAAAGTCGGTTGAAGGAGCATGAATCAAAAGAAAGAGAGTTTGAAGGCCAAGTGAAGGAGCTGGAATTTAAAAAGAAGCAATTCGAAAGCCAAATGGAGGAGCTCACATCAAATCTGTGGCAATTGAAAGGACAGGTTAAGGAACTTGAGTCCAAAGAGAAGCAGTTTGATAGCCGGGTGAAAGCATTTGAATCAAAAGAGGACGAATTTGAAGGCCGAGCGAAGGAGCATGAATCAAAAGAGAGAGAATTTGAAATTCAAGCGAAGGAGCTGGAATCTAAAAAGAAGCATTTTGAAAGCCAAGTGGAGGAGCTCACATCAAAACTGTGGCAATTGAAAGGACAAGTTAAGGAACTTGAGTACAAAGAGAAGCAATTTGATAGCCGGGTGAAAGCGTTTGAATCAAAAGAGGTTGAATTTAAAGATCGAGTGAGGGAGTTTGAAGCGAAGCAGTTGGAAGTGCAACTGAAGGAGCTAAAGTCAATGCAGAATGAATTTGATGGAGAACTGAAGGAGCTTGAGTTAAGAGAGGATCAATATGAAGCATTACTAAAATCATTTGACGAGGAAATTAAATCAGTTACATGTTACACAGATGATCAATCAACTGCTACCATTGATGGAAGAAGTTTGCAGTTGCTCCCAAGTGACGAAACTGAAATTCTAGATAATCTGCAAGGATTATCAGATCCATCAAAAGTTGTTTTGGATATAATACAGAATCCCATTATTCAAAAGTATAAGATGGGAGACAATGCTGTAATTATTGATGATAGAGACATTCTTCTGCTCAAACAACTGATGAGAATCAAACCACATATTAAACCTCGTGTAAGAGAAGAAGCAATGAAGCTAGCACTTAATTTGAAATCCAACATAAGTGAAAATACTGAAAATTCGGTGGCAGTTCTGGGTTTTCTACTCCTTGTGTCAATTTATGGATTGGCTCCTTCTTTCGATGAAGATGAAGTTTTAAAGCTTTTCGGGTTTGCTGCTCAGGACAAGATAGCTGTAGAGCTGTTTGGAACTCTGGGTTTTGCCGATAAAGCTTCTGATTTTGTTCAGAAACTTATCATGAAGAAACAAAATATTGAAGCTGTTAGATTCATTCGTGCTTATATAATGCCAACAAGAATCAAACCAACGATCTCTTGCATCCAAGAAAATCGTCTCCATTGA

Protein sequence

>Msa0221550

MNFEAFDKPKFCGNGDDDGRDHVSCEYFESEDDDVPLLPRFTRISSLVKRDVSVKKRDFEFDDNNSLPISAKKSKASLYHDHDKDEDDIPLLDLIKRPSKSTDKSFSSLKKGLALLESSFEEGNKKTELEENRLQSIKRDIEECRKELENKKKEISCFRGIDEAHEKLQGKIEECVKDFVVKEGQLNLMDDLIGERKLELKTKEIELNQVKGNISKEIELRQVIDNIDKDRERKEEELKAKERELDAMNKLISGKAEKLESKRKKLLKLTSEKENVRAQIKEFESIKKQLEGQVKELESKEKQCDERLEVLISREKQLEGHMKEFESKMQELEGRTMQLESKEKQVEGRAMELKLKEMQLKVQEKEFKSKEEKFEGLMKEVEFKKEHFENQSNVLESIDNQLVCQVKEFESKQKEFECQKKESIVRQKYFKSRLESGEKKHESRLKEHESKEREFEGQVKELEFKKKQFESQMEELTSNLWQLKGQVKELESKEKQFDSRVKAFESKEDEFEGRAKEHESKEREFEIQAKELESKKKHFESQVEELTSKLWQLKGQVKELEYKEKQFDSRVKAFESKEVEFKDRVREFEAKQLEVQLKELKSMQNEFDGELKELELREDQYEALLKSFDEEIKSVTCYTDDQSTATIDGRSLQLLPSDETEILDNLQGLSDPSKVVLDIIQNPIIQKYKMGDNAVIIDDRDILLLKQLMRIKPHIKPRVREEAMKLALNLKSNISENTENSVAVLGFLLLVSIYGLAPSFDEDEVLKLFGFAAQDKIAVELFGTLGFADKASDFVQKLIMKKQNIEAVRFIRAYIMPTRIKPTISCIQENRLH*