AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0221550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221550 MtrunA17_Chr2g0325861 88.542 864 63 4 1 833 2 860 0.0 1435
Msa0221550 MtrunA17_Chr2g0325321 51.411 815 293 10 28 795 3 761 0.0 609
Msa0221550 MtrunA17_Chr2g0325231 47.797 908 351 14 4 812 6 889 0.0 602
Msa0221550 MtrunA17_Chr2g0325711 45.377 822 316 13 35 814 26 756 0.0 542
Msa0221550 MtrunA17_Chr2g0325721 62.549 510 163 6 1 491 1 501 1.98e-168 497
Msa0221550 MtrunA17_Chr2g0325721 35.623 393 193 14 281 629 125 501 2.92e-24 107
Msa0221550 MtrunA17_Chr2g0325661 43.687 895 323 17 1 814 1 795 3.12e-158 484
Msa0221550 MtrunA17_Chr2g0325281 53.300 606 207 8 28 587 3 578 1.23e-157 473
Msa0221550 MtrunA17_Chr2g0325281 43.600 250 135 3 393 637 242 490 4.75e-34 139
Msa0221550 MtrunA17_Chr2g0325281 35.227 264 142 5 196 445 353 601 2.51e-16 83.2
Msa0221550 MtrunA17_Chr2g0325281 36.434 129 70 1 513 641 252 368 2.55e-11 67.4
Msa0221550 MtrunA17_Chr2g0280141 44.607 751 325 14 86 819 66 742 1.30e-156 478
Msa0221550 MtrunA17_Chr2g0325361 49.235 719 229 13 28 620 3 711 8.75e-153 475
Msa0221550 MtrunA17_Chr2g0325361 49.123 627 267 8 229 819 391 1001 7.31e-136 431
Msa0221550 MtrunA17_Chr2g0325361 40.237 169 97 2 471 637 262 428 3.89e-15 80.1
Msa0221550 MtrunA17_Chr2g0325691 42.120 736 310 13 4 637 5 726 1.08e-115 368
Msa0221550 MtrunA17_Chr2g0325221 43.248 622 243 15 1 544 1 590 2.42e-104 337
Msa0221550 MtrunA17_Chr2g0325811 42.647 544 239 10 326 814 226 751 5.17e-104 340
Msa0221550 MtrunA17_Chr2g0325811 42.185 595 248 11 1 526 1 568 2.28e-96 320
Msa0221550 MtrunA17_Chr2g0325761 39.011 728 259 15 1 648 1 623 6.31e-100 325
Msa0221550 MtrunA17_Chr6g0471251 42.500 560 261 9 281 814 162 686 7.11e-98 322
Msa0221550 MtrunA17_Chr6g0471251 42.569 545 192 15 1 491 2 479 4.95e-73 254
Msa0221550 MtrunA17_Chr2g0325611 48.990 396 172 6 450 830 144 524 4.75e-87 288
Msa0221550 MtrunA17_Chr2g0325611 38.235 306 117 5 198 477 57 316 5.72e-33 135
Msa0221550 MtrunA17_Chr2g0325611 28.190 337 203 7 18 348 7 310 2.44e-16 83.2
Msa0221550 MtrunA17_Chr2g0325351 47.209 430 172 7 1 397 2 409 1.41e-86 290
Msa0221550 MtrunA17_Chr2g0325351 48.756 402 179 5 437 814 209 607 9.09e-84 282
Msa0221550 MtrunA17_Chr2g0325701 42.235 528 224 10 283 792 125 589 2.51e-85 283
Msa0221550 MtrunA17_Chr2g0325701 41.990 412 185 9 4 384 5 393 9.22e-62 219
Msa0221550 MtrunA17_Chr5g0416271 48.189 359 170 5 444 799 145 490 1.16e-82 273
Msa0221550 MtrunA17_Chr5g0416271 41.477 352 160 6 49 392 15 328 7.23e-53 192
Msa0221550 MtrunA17_Chr2g0325621 45.547 494 224 12 361 821 106 587 6.83e-82 276
Msa0221550 MtrunA17_Chr2g0325621 49.630 405 162 6 32 421 13 390 9.59e-79 268
Msa0221550 MtrunA17_Chr2g0325651 42.718 412 169 8 7 417 21 366 8.72e-69 233
Msa0221550 MtrunA17_Chr2g0325651 50.327 153 65 2 445 586 236 388 4.26e-26 111
Msa0221550 MtrunA17_Chr2g0325651 43.382 136 68 1 501 627 236 371 5.63e-20 93.2
Msa0221550 MtrunA17_Chr2g0333341 41.350 474 191 9 1 412 1 449 2.83e-68 238
Msa0221550 MtrunA17_Chr2g0333341 40.129 466 234 11 374 799 154 614 1.85e-66 233
Msa0221550 MtrunA17_Chr7g0235901 43.896 385 131 7 87 471 12 311 9.70e-62 211
Msa0221550 MtrunA17_Chr7g0235901 51.613 186 69 3 405 569 126 311 9.56e-33 129
Msa0221550 MtrunA17_Chr7g0235901 42.786 201 91 4 452 642 112 298 4.40e-23 100
Msa0221550 MtrunA17_Chr2g0325291 70.000 170 43 2 653 814 1 170 1.88e-61 207
Msa0221550 MtrunA17_Chr2g0325781 45.511 323 131 12 1 299 1 302 8.53e-60 205
Msa0221550 MtrunA17_Chr2g0325781 54.639 97 34 1 465 561 215 301 2.09e-17 84.0
Msa0221550 MtrunA17_Chr2g0325781 44.828 174 76 8 330 492 138 302 2.52e-17 84.0
Msa0221550 MtrunA17_Chr2g0325781 31.707 246 140 8 376 615 78 301 5.57e-11 64.7
Msa0221550 MtrunA17_Chr2g0325781 48.352 91 43 1 450 536 214 304 8.46e-11 64.3
Msa0221550 MtrunA17_Chr5g0434741 41.484 364 137 8 1 348 1 304 1.05e-58 202
Msa0221550 MtrunA17_Chr2g0325261 42.005 369 129 8 1 349 2 305 5.66e-58 200
Msa0221550 MtrunA17_Chr2g0325261 47.059 85 45 0 487 571 222 306 1.49e-11 66.2
Msa0221550 MtrunA17_Chr2g0325341 42.005 369 129 8 1 349 2 305 5.66e-58 200
Msa0221550 MtrunA17_Chr2g0325341 47.059 85 45 0 487 571 222 306 1.49e-11 66.2
Msa0221550 MtrunA17_Chr2g0332961 48.485 297 130 6 473 765 76 353 2.66e-56 197
Msa0221550 MtrunA17_Chr2g0332961 35.907 259 115 10 60 306 10 229 5.88e-16 80.5
Msa0221550 MtrunA17_Chr2g0332961 37.634 186 90 3 258 442 68 228 1.33e-11 67.0
Msa0221550 MtrunA17_Chr2g0325741 48.173 301 113 10 1 266 2 294 4.81e-55 192
Msa0221550 MtrunA17_Chr2g0325241 45.455 297 147 3 434 717 130 424 7.45e-55 196
Msa0221550 MtrunA17_Chr2g0325241 36.818 220 114 4 286 480 119 338 2.95e-18 88.2
Msa0221550 MtrunA17_Chr2g0325241 32.020 203 117 3 229 431 128 309 3.06e-12 69.7
Msa0221550 MtrunA17_Chr5g0434761 57.377 183 70 1 640 814 2 184 1.53e-54 189
Msa0221550 MtrunA17_Chr3g0140591 60.119 168 58 2 636 795 6 172 8.89e-50 172
Msa0221550 MtrunA17_Chr2g0332951 52.727 220 82 7 581 792 2 207 3.36e-49 172
Msa0221550 MtrunA17_Chr2g0325771 65.217 161 54 1 634 792 12 172 4.18e-48 168
Msa0221550 MtrunA17_Chr2g0325641 45.018 271 130 3 378 629 1 271 4.64e-45 165
Msa0221550 MtrunA17_Chr2g0325641 40.419 334 164 4 243 569 2 307 1.23e-41 155
Msa0221550 MtrunA17_Chr2g0325641 39.385 358 173 5 280 637 1 314 1.48e-37 143
Msa0221550 MtrunA17_Chr2g0325641 34.795 342 144 11 195 499 8 307 2.46e-18 87.4
Msa0221550 MtrunA17_Chr2g0325641 35.714 154 80 1 487 640 1 135 6.04e-12 67.8
Msa0221550 MtrunA17_Chr2g0325631 57.377 183 70 1 640 814 2 184 1.03e-43 159
Msa0221550 MtrunA17_Chr2g0325201 45.378 238 88 4 152 360 2 226 8.40e-39 144
Msa0221550 MtrunA17_Chr8g0361651 36.443 343 168 8 478 814 26 324 1.57e-37 147
Msa0221550 MtrunA17_Chr2g0325331 55.556 153 60 1 641 785 3 155 3.54e-36 134
Msa0221550 MtrunA17_Chr5g0434751 39.450 327 147 4 311 637 4 279 6.00e-30 120
Msa0221550 MtrunA17_Chr5g0434751 39.062 256 117 4 434 650 4 259 8.70e-30 120
Msa0221550 MtrunA17_Chr5g0434751 41.176 289 152 2 280 568 1 271 5.55e-29 117
Msa0221550 MtrunA17_Chr5g0434751 51.049 143 68 1 501 641 1 143 5.15e-21 94.4
Msa0221550 MtrunA17_Chr5g0434751 35.714 280 144 3 230 498 17 271 1.28e-17 84.3
Msa0221550 MtrunA17_Chr4g0024061 28.551 704 342 22 135 814 5 571 6.19e-28 120
Msa0221550 MtrunA17_Chr4g0044221 52.941 136 44 5 610 732 168 296 1.57e-27 114
Msa0221550 MtrunA17_Chr4g0044221 34.659 176 111 3 130 301 15 190 2.01e-14 75.5
Msa0221550 MtrunA17_Chr2g0325801 42.424 165 55 4 1 165 2 126 7.06e-23 94.7
Msa0221550 MtrunA17_Chr7g0235921 61.538 104 39 1 709 812 1 103 3.29e-22 92.4
Msa0221550 MtrunA17_Chr2g0325751 45.455 154 61 3 483 634 3 135 7.03e-22 93.2
Msa0221550 MtrunA17_Chr2g0325751 46.617 133 64 1 443 575 5 130 3.17e-20 88.2
Msa0221550 MtrunA17_Chr2g0325751 38.554 166 74 2 275 440 2 139 6.88e-15 73.2
Msa0221550 MtrunA17_Chr3g0140581 36.129 155 99 0 106 260 1 155 4.07e-19 85.1
Msa0221550 MtrunA17_Chr2g0325791 40.123 162 86 3 418 575 2 156 1.47e-17 84.7
Msa0221550 MtrunA17_Chr2g0325791 42.657 143 61 3 411 546 41 169 3.90e-15 77.4
Msa0221550 MtrunA17_Chr2g0325791 33.333 186 80 4 295 476 24 169 8.28e-11 64.3
Msa0221550 MtrunA17_Chr2g0325211 34.946 186 101 3 454 637 2 169 1.63e-13 70.1
Msa0221550 MtrunA17_Chr2g0325211 35.165 182 89 2 345 526 2 154 8.04e-11 62.0
Msa0221550 MtrunA17_Chr2g0325731 68.627 51 16 0 508 558 8 58 2.50e-11 60.1
Msa0221550 MtrunA17_Chr2g0325731 61.818 55 21 0 434 488 4 58 3.42e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221550 AT5G27220.4 25.932 644 405 17 171 791 208 802 2.19e-16 84.7
Msa0221550 AT5G27220.2 25.932 644 405 17 171 791 208 802 2.19e-16 84.7
Msa0221550 AT5G27220.1 26.138 681 407 20 134 791 195 802 3.64e-16 84.0
Msa0221550 AT5G27220.3 26.138 681 407 20 134 791 195 802 3.64e-16 84.0

Find 160 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACTCCTTGTGTCAATTTA+TGG 0.080642 2_1:+70175871 Msa0221550:CDS
CTCCTCGATATCTCGCTTTA+TGG 0.106596 2_1:-70173374 None:intergenic
TGAAAATTCGGTGGCAGTTC+TGG 0.150498 2_1:+70175843 Msa0221550:CDS
CTTTAGCAACTTCTTCCTTT+TGG 0.223344 2_1:-70173891 None:intergenic
CGTTGTTAGATTTAATTAAA+AGG 0.247437 2_1:+70173236 Msa0221550:CDS
TGCATCAAGCTCTCTCTCTT+TGG 0.269901 2_1:-70173828 None:intergenic
TCATTTGCACATTGAGCATT+AGG 0.279697 2_1:-70176801 None:intergenic
TTTGATTTCTTAGCAGAAAT+AGG 0.283122 2_1:-70173169 None:intergenic
AGATCCATCAAAAGTTGTTT+TGG 0.291459 2_1:+70175639 Msa0221550:CDS
GTTTACCCGGATTTCGAGTT+TGG 0.322265 2_1:+70173087 Msa0221550:CDS
AGAGCTTGAAAATAAGAAAA+AGG 0.324967 2_1:+70173405 Msa0221550:CDS
TGTAGAGCTGTTTGGAACTC+TGG 0.329670 2_1:+70175963 Msa0221550:CDS
CAATCAACTGCTACCATTGA+TGG 0.335095 2_1:+70175553 Msa0221550:CDS
GAAGGAGGTGGAGTTTAAAA+AGG 0.337333 2_1:+70174243 Msa0221550:CDS
GTTCCTCTTCCTTCCTTTCA+CGG 0.342021 2_1:-70173657 None:intergenic
TTACTATCGACTCCTTCTTT+TGG 0.343808 2_1:-70174363 None:intergenic
TAACATACCAGAAGCTTTAT+CGG 0.346500 2_1:-70175992 None:intergenic
GCTATCAAACTGCTTCTCTT+TGG 0.353127 2_1:-70174584 None:intergenic
CCTTGTGTCAATTTATGGAT+TGG 0.361905 2_1:+70175876 Msa0221550:CDS
TAAAATAACCTTGATTTCAA+TGG 0.363499 2_1:-70176682 None:intergenic
TTGATGTGAGCTCCTCCATT+TGG 0.367926 2_1:-70174522 None:intergenic
GAATGAGAAGATTCTCAAAC+AGG 0.370642 2_1:+70176883 Msa0221550:three_prime_UTR
AAGTGAAAATACTGAAAATT+CGG 0.388056 2_1:+70175831 Msa0221550:CDS
GAAAATTCGGTGGCAGTTCT+GGG 0.395040 2_1:+70175844 Msa0221550:CDS
ATCGTTGGTTTGATTCTTGT+TGG 0.397145 2_1:-70176635 None:intergenic
GAATGTTCGTGCTCAAATAA+AGG 0.401392 2_1:+70173931 Msa0221550:CDS
AGAATTTCAGTTTCGTCACT+TGG 0.405145 2_1:-70175595 None:intergenic
TTTGACGAGGAAATTAAATC+AGG 0.407950 2_1:+70174991 Msa0221550:CDS
TAAACTCCACCTCCTTCATT+AGG 0.415653 2_1:-70174237 None:intergenic
TTCAAAAGCCGGCTCGAGTC+AGG 0.421084 2_1:+70174397 Msa0221550:CDS
AATATGTGGACTGTGAAATC+AGG 0.425338 2_1:+70176770 Msa0221550:three_prime_UTR
TTGATTCATGCTCCTTCGCT+CGG 0.428334 2_1:-70174648 None:intergenic
TTGATTTAAACTCCTTCTCC+TGG 0.429349 2_1:-70174195 None:intergenic
AAGATAGCTGTAGAGCTGTT+TGG 0.433585 2_1:+70175955 Msa0221550:CDS
GGAAGGGCAGCTCAATTTGA+TGG 0.433892 2_1:+70173513 Msa0221550:CDS
GTAGAGCTGTTTGGAACTCT+GGG 0.438058 2_1:+70175964 Msa0221550:CDS
AAAGAAGCAACTTGAAGGTC+AGG 0.440427 2_1:+70173967 Msa0221550:CDS
AATTTGATGGATGACTTGAT+TGG 0.441045 2_1:+70173526 Msa0221550:CDS
TGCGGTAATGGTGATGATGA+TGG 0.443280 2_1:+70173001 Msa0221550:CDS
AAAATGCAAAATACAAGAAA+AGG 0.451647 2_1:-70176937 None:intergenic
CGAAGCTCATGAGAAATTGC+AGG 0.452129 2_1:+70173453 Msa0221550:CDS
TTGATTCAAACTCCTTTACT+TGG 0.452256 2_1:-70174321 None:intergenic
CTTACACGAGGTTTAATATG+TGG 0.453106 2_1:-70175766 None:intergenic
GATTTATCAGTTGATTTGCT+TGG 0.454214 2_1:-70173259 None:intergenic
TTGAATTTAAAGATCGAGTG+AGG 0.457501 2_1:+70174845 Msa0221550:CDS
TAAATTCCAGCTCCTTCACT+TGG 0.464188 2_1:-70174480 None:intergenic
TCAATAAAGAAGCAACTTGA+AGG 0.464485 2_1:+70173962 Msa0221550:CDS
TCACCATTACCGCAAAACTT+TGG 0.464980 2_1:-70172992 None:intergenic
TCAAGAGAGAAGCAACTTGA+AGG 0.467554 2_1:+70174046 Msa0221550:CDS
TCTTCTCTCCTGACTCGAGC+CGG 0.469898 2_1:-70174405 None:intergenic
AACGTGCAGGTGCATGAAGA+TGG 0.473081 2_1:-70176736 None:intergenic
CACATTGAGCATTAGGAGGA+TGG 0.474253 2_1:-70176794 None:intergenic
AATCGTCTCCATTGAAATCA+AGG 0.477245 2_1:+70176674 Msa0221550:exon
TTGATGTGAGCTCCTCCACT+TGG 0.478027 2_1:-70174732 None:intergenic
GCTAAAGCTAACATCAGAAA+AGG 0.480977 2_1:+70173907 Msa0221550:CDS
TCAATGCAGAATGAATTTGA+TGG 0.481556 2_1:+70174913 Msa0221550:CDS
GCAATTGAAAGGACAAGTTA+AGG 0.483479 2_1:+70174762 Msa0221550:CDS
GAAGGAGTTTAAATCAAAAG+AGG 0.484213 2_1:+70174201 Msa0221550:CDS
CCTTTACTTGGCATACAAGT+TGG 0.488568 2_1:-70174309 None:intergenic
CTTTGTTACCGAGGTTTACC+CGG 0.489728 2_1:+70173074 Msa0221550:CDS
CATTATTCAAAAGTATAAGA+TGG 0.494090 2_1:+70175678 Msa0221550:CDS
GCAATTGAAAGGACAGGTTA+AGG 0.494228 2_1:+70174552 Msa0221550:CDS
AGTCATTGATAATATTGATA+AGG 0.496791 2_1:+70173633 Msa0221550:CDS
GCAATGAACAAATTAATTAG+TGG 0.497881 2_1:+70173848 Msa0221550:CDS
TGAAATTCAAGCGAAGGAGC+TGG 0.502421 2_1:+70174684 Msa0221550:CDS
TCTAAGATGCAGGAACTTGA+AGG 0.507887 2_1:+70174088 Msa0221550:CDS
GGAGTTTGAAGCGAAGCAGT+TGG 0.508930 2_1:+70174867 Msa0221550:CDS
CTGCAAACTTCTTCCATCAA+TGG 0.511498 2_1:-70175566 None:intergenic
AAGAGAAGCAATTTGATAGC+CGG 0.511794 2_1:+70174797 Msa0221550:CDS
CAATGTGCAAATGAATTAGC+AGG 0.514188 2_1:+70176810 Msa0221550:three_prime_UTR
GAAACATGAAAGTCGGTTGA+AGG 0.517920 2_1:+70174426 Msa0221550:CDS
AGAGAAGCAGTTTGATAGCC+GGG 0.518876 2_1:+70174588 Msa0221550:CDS
GGAATTTGAATGCCAAAAGA+AGG 0.520295 2_1:+70174351 Msa0221550:CDS
GAAGCAATTCGAAAGCCAAA+TGG 0.522174 2_1:+70174507 Msa0221550:CDS
AGAATTTGAAATTCAAGCGA+AGG 0.522189 2_1:+70174678 Msa0221550:CDS
TCAAAAGAGGAGAAGTTTGA+AGG 0.522468 2_1:+70174214 Msa0221550:CDS
GTTGGTTGTAAATTAATATG+TGG 0.525022 2_1:+70176756 Msa0221550:three_prime_UTR
AGAGAAGCAATTTGATAGCC+GGG 0.528136 2_1:+70174798 Msa0221550:CDS
TCAAAAGAAAGAGAGTTTGA+AGG 0.528477 2_1:+70174457 Msa0221550:CDS
GAATTTCAGTTTCGTCACTT+GGG 0.529088 2_1:-70175594 None:intergenic
CCAACTTGTATGCCAAGTAA+AGG 0.529486 2_1:+70174309 Msa0221550:CDS
TTGATTCAAACGCTTTCACC+CGG 0.529873 2_1:-70174816 None:intergenic
TTGACTCTAACACATTCGAT+TGG 0.531740 2_1:-70174279 None:intergenic
AGAAATGCAACTTAAAGTCC+AGG 0.531894 2_1:+70174177 Msa0221550:CDS
CAATGAACAAATTAATTAGT+GGG 0.531973 2_1:+70173849 Msa0221550:CDS
ATTGTTAGAAAGCTCATTTG+AGG 0.533517 2_1:+70173309 Msa0221550:CDS
TGATGTTTCTGTTAAGAAGA+GGG 0.535516 2_1:+70173117 Msa0221550:CDS
GGAGTTTGAATCTAAGATGC+AGG 0.536802 2_1:+70174078 Msa0221550:CDS
GAAGCAATGTGATGAACGGC+TGG 0.539147 2_1:+70174012 Msa0221550:CDS
AGAGAAGCAAGTCGAAGGAC+GGG 0.541425 2_1:+70174135 Msa0221550:CDS
TTAATTTACAACCAACGTGC+AGG 0.543527 2_1:-70176749 None:intergenic
CGAATTTGAAGGCCGAGCGA+AGG 0.543642 2_1:+70174636 Msa0221550:CDS
AAGAGAAGCAGTTTGATAGC+CGG 0.545775 2_1:+70174587 Msa0221550:CDS
TCAAAACTGTGGCAATTGAA+AGG 0.545936 2_1:+70174751 Msa0221550:CDS
ACTTCATCTTCATCGAAAGA+AGG 0.546038 2_1:-70175901 None:intergenic
GAAGTTTGAAGGCCTAATGA+AGG 0.546338 2_1:+70174225 Msa0221550:CDS
GCAAGTCGAAGGACGGGCGA+TGG 0.547167 2_1:+70174141 Msa0221550:CDS
GCGGTAATGGTGATGATGAT+GGG 0.548173 2_1:+70173002 Msa0221550:CDS
ATTATTCAAAAGTATAAGAT+GGG 0.548310 2_1:+70175679 Msa0221550:CDS
TCAAAAGAGGACGAATTTGA+AGG 0.549498 2_1:+70174625 Msa0221550:CDS
CTCGAAATCCGGGTAAACCT+CGG 0.550108 2_1:-70173082 None:intergenic
TGAATTTGATGGAGAACTGA+AGG 0.552236 2_1:+70174924 Msa0221550:CDS
GCAAAATACAAGAAAAGGAA+AGG 0.555119 2_1:-70176932 None:intergenic
TCAAATCTGTGGCAATTGAA+AGG 0.559650 2_1:+70174541 Msa0221550:CDS
GATAGAACTTAATCAAGTCA+AGG 0.560272 2_1:+70173579 Msa0221550:CDS
TGATAAGGACCGTGAAAGGA+AGG 0.560715 2_1:+70173648 Msa0221550:CDS
TTAGAAAGCTCATTTGAGGA+AGG 0.561900 2_1:+70173313 Msa0221550:CDS
ACGTGCAGGTGCATGAAGAT+GGG 0.565339 2_1:-70176735 None:intergenic
GAAAGCATTTGAATCAAAAG+AGG 0.571886 2_1:+70174612 Msa0221550:CDS
GTGATGTTTCTGTTAAGAAG+AGG 0.573928 2_1:+70173116 Msa0221550:CDS
TTTGCACATTGAGCATTAGG+AGG 0.574704 2_1:-70176798 None:intergenic
GAAAGCGTTTGAATCAAAAG+AGG 0.576259 2_1:+70174822 Msa0221550:CDS
GTCCATAAAGCGAGATATCG+AGG 0.577796 2_1:+70173372 Msa0221550:CDS
ATAGAACTTAATCAAGTCAA+GGG 0.578049 2_1:+70173580 Msa0221550:CDS
TCTTGGATGCAAGAGATCGT+TGG 0.579943 2_1:-70176650 None:intergenic
AAGAGAAGCAAGTCGAAGGA+CGG 0.580246 2_1:+70174134 Msa0221550:CDS
TGAAGGCCAAGTGAAGGAGC+TGG 0.581942 2_1:+70174474 Msa0221550:CDS
ATGAGCTTTCTAACAATGCA+AGG 0.582002 2_1:-70173303 None:intergenic
GGAGTTTGAATCAAAGCAGA+AGG 0.582316 2_1:+70174330 Msa0221550:CDS
TTGATTCAAATGCTTTCACC+CGG 0.583537 2_1:-70174606 None:intergenic
GCAACTTGAAGGACACATGA+AGG 0.585195 2_1:+70174057 Msa0221550:CDS
GGACCGTGAAAGGAAGGAAG+AGG 0.585661 2_1:+70173654 Msa0221550:CDS
TGAAGGCCTAATGAAGGAGG+TGG 0.589996 2_1:+70174231 Msa0221550:CDS
TATGCATATATATTTCTCGA+AGG 0.591977 2_1:+70176962 Msa0221550:three_prime_UTR
AGAGTTTGAAGGCCAAGTGA+AGG 0.594285 2_1:+70174468 Msa0221550:CDS
TGAATTTAAAGATCGAGTGA+GGG 0.599120 2_1:+70174846 Msa0221550:CDS
TCAAAAGAGAAGCAAGTCGA+AGG 0.600747 2_1:+70174130 Msa0221550:CDS
TGCGTGGATCAATAAAGAGA+GGG 0.601127 2_1:-70176838 None:intergenic
ATGATGTTCCTTTGTTACCG+AGG 0.602142 2_1:+70173065 Msa0221550:CDS
CGGGTAAACCTCGGTAACAA+AGG 0.604343 2_1:-70173073 None:intergenic
GCAACTTAAAGTCCAGGAGA+AGG 0.605338 2_1:+70174183 Msa0221550:CDS
GCAACTTGAAGGTCAGGTCA+AGG 0.608454 2_1:+70173973 Msa0221550:CDS
TTTCTTAGCAGAAATAGGCA+AGG 0.610939 2_1:-70173164 None:intergenic
GAGAGAAGAAACATGAAAGT+CGG 0.614493 2_1:+70174419 Msa0221550:CDS
AAGAGAAGCAATGTGATGAA+CGG 0.614667 2_1:+70174008 Msa0221550:CDS
TTGCGTGGATCAATAAAGAG+AGG 0.615054 2_1:-70176839 None:intergenic
TGAGGAAGGCAATAAGAAGA+CGG 0.615201 2_1:+70173327 Msa0221550:CDS
TGAAAATACTGAAAATTCGG+TGG 0.615980 2_1:+70175834 Msa0221550:CDS
GCAGTTGGAAGTGCAACTGA+AGG 0.616833 2_1:+70174882 Msa0221550:CDS
ATATTGATAAGGACCGTGAA+AGG 0.619247 2_1:+70173644 Msa0221550:CDS
ATCTTCATGCACCTGCACGT+TGG 0.624742 2_1:+70176738 Msa0221550:three_prime_UTR
TCTGTGGCAATTGAAAGGAC+AGG 0.625484 2_1:+70174546 Msa0221550:CDS
GAAATTCTAGATAATCTGCA+AGG 0.625701 2_1:+70175610 Msa0221550:CDS
TATTTCTGCTAAGAAATCAA+AGG 0.627001 2_1:+70173171 Msa0221550:CDS
AAGGAAGGAAGAGGAACTCA+AGG 0.628919 2_1:+70173663 Msa0221550:CDS
GAAGCTCATGAGAAATTGCA+GGG 0.629107 2_1:+70173454 Msa0221550:CDS
GAAGGAGCTTGAGTTAAGAG+AGG 0.633737 2_1:+70174942 Msa0221550:CDS
TGCAAGGCCTTTCTTCAGCG+AGG 0.638330 2_1:-70173287 None:intergenic
GCAATTCGAAAGCCAAATGG+AGG 0.643602 2_1:+70174510 Msa0221550:CDS
TAATGGTGATGATGATGGGA+GGG 0.651761 2_1:+70173006 Msa0221550:CDS
TACAAGAAAAGGAAAGGACA+CGG 0.654271 2_1:-70176926 None:intergenic
CCAATCCATAAATTGACACA+AGG 0.656897 2_1:-70175876 None:intergenic
ATTGCTTCTTCTCTTACACG+AGG 0.664142 2_1:-70175778 None:intergenic
AATGAGAAGATTCTCAAACA+GGG 0.664747 2_1:+70176884 Msa0221550:three_prime_UTR
AGGAGCTCACATCAAATCTG+TGG 0.671175 2_1:+70174530 Msa0221550:CDS
GTTTGAAGGCCTAATGAAGG+AGG 0.671285 2_1:+70174228 Msa0221550:CDS
GTAATGGTGATGATGATGGG+AGG 0.675360 2_1:+70173005 Msa0221550:CDS
ATTACTAAAATCATTTGACG+AGG 0.688803 2_1:+70174978 Msa0221550:CDS
GCAATGTGATGAACGGCTGG+AGG 0.703208 2_1:+70174015 Msa0221550:CDS
AGGAGCTCACATCAAAACTG+TGG 0.719251 2_1:+70174740 Msa0221550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATTAAATCTAACAAC+GGG - chr2_1:70173237-70173256 None:intergenic 15.0%
!!! CTTTTAATTAAATCTAACAA+CGG - chr2_1:70173238-70173257 None:intergenic 15.0%
!! ATTAAAAGATAAAGTTGAAA+TGG - chr2_1:70175053-70175072 None:intergenic 15.0%
!!! ACTTTATCTTTTAATATCTT+AGG + chr2_1:70175058-70175077 Msa0221550:intron 15.0%
!! ACACATATATTTAAATGTTT+GGG - chr2_1:70175407-70175426 None:intergenic 15.0%
!!! ATCTTATACTTTTGAATAAT+GGG - chr2_1:70175679-70175698 None:intergenic 15.0%
!!! ATTATTCAAAAGTATAAGAT+GGG + chr2_1:70175679-70175698 Msa0221550:CDS 15.0%
!! CGTTGTTAGATTTAATTAAA+AGG + chr2_1:70173236-70173255 Msa0221550:CDS 20.0%
!! AAAAATTGAAGAATGTGTTA+AGG + chr2_1:70173477-70173496 Msa0221550:CDS 20.0%
!!! AGTCATTGATAATATTGATA+AGG + chr2_1:70173633-70173652 Msa0221550:CDS 20.0%
!! CAATGAACAAATTAATTAGT+GGG + chr2_1:70173849-70173868 Msa0221550:CDS 20.0%
!! CACACATATATTTAAATGTT+TGG - chr2_1:70175408-70175427 None:intergenic 20.0%
!!! TACTGATGTTTCTATAAATA+TGG + chr2_1:70175487-70175506 Msa0221550:intron 20.0%
!!! ACTGATGTTTCTATAAATAT+GGG + chr2_1:70175488-70175507 Msa0221550:intron 20.0%
!!! CATCTTATACTTTTGAATAA+TGG - chr2_1:70175680-70175699 None:intergenic 20.0%
!!! CATTATTCAAAAGTATAAGA+TGG + chr2_1:70175678-70175697 Msa0221550:CDS 20.0%
!! AAGTGAAAATACTGAAAATT+CGG + chr2_1:70175831-70175850 Msa0221550:CDS 20.0%
!! AAGAAAATAAGTATAATAGC+TGG - chr2_1:70176043-70176062 None:intergenic 20.0%
!! ATGATTAAGATATTATCTGT+TGG + chr2_1:70176152-70176171 Msa0221550:intron 20.0%
!!! TCTATTTCTTAAAATGTTTG+AGG + chr2_1:70176412-70176431 Msa0221550:intron 20.0%
!! TAAAATAACCTTGATTTCAA+TGG - chr2_1:70176685-70176704 None:intergenic 20.0%
!! AAAATGCAAAATACAAGAAA+AGG - chr2_1:70176940-70176959 None:intergenic 20.0%
!! TTTGATTTCTTAGCAGAAAT+AGG - chr2_1:70173172-70173191 None:intergenic 25.0%
!! TATTTCTGCTAAGAAATCAA+AGG + chr2_1:70173171-70173190 Msa0221550:CDS 25.0%
! AGAGCTTGAAAATAAGAAAA+AGG + chr2_1:70173405-70173424 Msa0221550:CDS 25.0%
!!! AAGGAAATTAGTTGTTTTAG+AGG + chr2_1:70173424-70173443 Msa0221550:CDS 25.0%
! ATAGAACTTAATCAAGTCAA+GGG + chr2_1:70173580-70173599 Msa0221550:CDS 25.0%
!! NNNAATTGCTGAATTTGCTT+TGG + chr2_1:70173796-70173815 Msa0221550:intron 25.0%
! GCAATGAACAAATTAATTAG+TGG + chr2_1:70173848-70173867 Msa0221550:CDS 25.0%
! GACAAAAATATTTCAAAAGC+CGG + chr2_1:70174386-70174405 Msa0221550:CDS 25.0%
! ATTACTAAAATCATTTGACG+AGG + chr2_1:70174978-70174997 Msa0221550:CDS 25.0%
!!! TGAGTTTCAAGGTTTTTTTT+TGG + chr2_1:70175201-70175220 Msa0221550:intron 25.0%
! ATAGAAACATCAGTAAATCA+AGG - chr2_1:70175482-70175501 None:intergenic 25.0%
!! ATATCCAAAACAACTTTTGA+TGG - chr2_1:70175646-70175665 None:intergenic 25.0%
!! TTCAGTATTTTCACTTATGT+TGG - chr2_1:70175827-70175846 None:intergenic 25.0%
!!! GATGAAGTTTTAAAGCTTTT+CGG + chr2_1:70175916-70175935 Msa0221550:CDS 25.0%
!!! ATGAAGTTTTAAAGCTTTTC+GGG + chr2_1:70175917-70175936 Msa0221550:CDS 25.0%
! TTAAGATATTATCTGTTGGT+AGG + chr2_1:70176156-70176175 Msa0221550:intron 25.0%
!!! GTTTTGTTGAAGTGAAATTT+TGG + chr2_1:70176529-70176548 Msa0221550:intron 25.0%
! GTTGGTTGTAAATTAATATG+TGG + chr2_1:70176756-70176775 Msa0221550:three_prime_UTR 25.0%
! TATGCATATATATTTCTCGA+AGG + chr2_1:70176962-70176981 Msa0221550:three_prime_UTR 25.0%
! TTTGATAAGCCAAAGTTTTG+CGG + chr2_1:70172983-70173002 Msa0221550:CDS 30.0%
! TGATGTTTCTGTTAAGAAGA+GGG + chr2_1:70173117-70173136 Msa0221550:CDS 30.0%
!! GATTTATCAGTTGATTTGCT+TGG - chr2_1:70173262-70173281 None:intergenic 30.0%
ATTGTTAGAAAGCTCATTTG+AGG + chr2_1:70173309-70173328 Msa0221550:CDS 30.0%
! TGTTAAGGATTTTGTAGTGA+AGG + chr2_1:70173492-70173511 Msa0221550:CDS 30.0%
! AATTTGATGGATGACTTGAT+TGG + chr2_1:70173526-70173545 Msa0221550:CDS 30.0%
GATAGAACTTAATCAAGTCA+AGG + chr2_1:70173579-70173598 Msa0221550:CDS 30.0%
!! TGAATTTGCTTTGGAACTTA+AGG + chr2_1:70173805-70173824 Msa0221550:intron 30.0%
CAGAAAAATTAGAGTCCAAA+AGG + chr2_1:70173876-70173895 Msa0221550:CDS 30.0%
TCAATAAAGAAGCAACTTGA+AGG + chr2_1:70173962-70173981 Msa0221550:CDS 30.0%
GAAGGAGTTTAAATCAAAAG+AGG + chr2_1:70174201-70174220 Msa0221550:CDS 30.0%
TTGATTCAAACTCCTTTACT+TGG - chr2_1:70174324-70174343 None:intergenic 30.0%
TCAAAAGAAAGAGAGTTTGA+AGG + chr2_1:70174457-70174476 Msa0221550:CDS 30.0%
!! GAAAGCATTTGAATCAAAAG+AGG + chr2_1:70174612-70174631 Msa0221550:CDS 30.0%
! AGAATTTGAAATTCAAGCGA+AGG + chr2_1:70174678-70174697 Msa0221550:CDS 30.0%
TTGAATTTAAAGATCGAGTG+AGG + chr2_1:70174845-70174864 Msa0221550:CDS 30.0%
TGAATTTAAAGATCGAGTGA+GGG + chr2_1:70174846-70174865 Msa0221550:CDS 30.0%
TCAATGCAGAATGAATTTGA+TGG + chr2_1:70174913-70174932 Msa0221550:CDS 30.0%
TTTGACGAGGAAATTAAATC+AGG + chr2_1:70174991-70175010 Msa0221550:CDS 30.0%
!!! AGGTTTTATTCTTGTCGAAT+CGG + chr2_1:70175078-70175097 Msa0221550:intron 30.0%
TATGATATGACTGAGTTTCA+AGG + chr2_1:70175190-70175209 Msa0221550:intron 30.0%
!!! TTCAAGGTTTTTTTTTGGAG+AGG + chr2_1:70175206-70175225 Msa0221550:intron 30.0%
! TCAGTTGATACTAACAGTAA+CGG - chr2_1:70175375-70175394 None:intergenic 30.0%
GAAATTCTAGATAATCTGCA+AGG + chr2_1:70175610-70175629 Msa0221550:CDS 30.0%
! AGATCCATCAAAAGTTGTTT+TGG + chr2_1:70175639-70175658 Msa0221550:CDS 30.0%
TGAAAATACTGAAAATTCGG+TGG + chr2_1:70175834-70175853 Msa0221550:CDS 30.0%
! TAACATACCAGAAGCTTTAT+CGG - chr2_1:70175995-70176014 None:intergenic 30.0%
ATATATCATGTAGAGTCTCT+AGG - chr2_1:70176119-70176138 None:intergenic 30.0%
AAGCTGAACTGATATGTAAT+TGG + chr2_1:70176179-70176198 Msa0221550:intron 30.0%
!!! TCTGCTTAGTTTTGGTTAAT+TGG + chr2_1:70176480-70176499 Msa0221550:intron 30.0%
AATGAGAAGATTCTCAAACA+GGG + chr2_1:70176884-70176903 Msa0221550:three_prime_UTR 30.0%
GCAAAATACAAGAAAAGGAA+AGG - chr2_1:70176935-70176954 None:intergenic 30.0%
! TTTTACCAAACTCGAAATCC+GGG - chr2_1:70173095-70173114 None:intergenic 35.0%
! GTTTTACCAAACTCGAAATC+CGG - chr2_1:70173096-70173115 None:intergenic 35.0%
! GTGATGTTTCTGTTAAGAAG+AGG + chr2_1:70173116-70173135 Msa0221550:CDS 35.0%
TTTCTTAGCAGAAATAGGCA+AGG - chr2_1:70173167-70173186 None:intergenic 35.0%
ATGAGCTTTCTAACAATGCA+AGG - chr2_1:70173306-70173325 None:intergenic 35.0%
TTAGAAAGCTCATTTGAGGA+AGG + chr2_1:70173313-70173332 Msa0221550:CDS 35.0%
! AAGGATTTTGTAGTGAAGGA+AGG + chr2_1:70173496-70173515 Msa0221550:CDS 35.0%
! AGGATTTTGTAGTGAAGGAA+GGG + chr2_1:70173497-70173516 Msa0221550:CDS 35.0%
ATATTGATAAGGACCGTGAA+AGG + chr2_1:70173644-70173663 Msa0221550:CDS 35.0%
! CTTTAGCAACTTCTTCCTTT+TGG - chr2_1:70173894-70173913 None:intergenic 35.0%
GCTAAAGCTAACATCAGAAA+AGG + chr2_1:70173907-70173926 Msa0221550:CDS 35.0%
GAATGTTCGTGCTCAAATAA+AGG + chr2_1:70173931-70173950 Msa0221550:CDS 35.0%
AAGAGAAGCAATGTGATGAA+CGG + chr2_1:70174008-70174027 Msa0221550:CDS 35.0%
AGAAATGCAACTTAAAGTCC+AGG + chr2_1:70174177-70174196 Msa0221550:CDS 35.0%
TTGATTTAAACTCCTTCTCC+TGG - chr2_1:70174198-70174217 None:intergenic 35.0%
TCAAAAGAGGAGAAGTTTGA+AGG + chr2_1:70174214-70174233 Msa0221550:CDS 35.0%
TTGACTCTAACACATTCGAT+TGG - chr2_1:70174282-70174301 None:intergenic 35.0%
GGAATTTGAATGCCAAAAGA+AGG + chr2_1:70174351-70174370 Msa0221550:CDS 35.0%
!!! TTACTATCGACTCCTTCTTT+TGG - chr2_1:70174366-70174385 None:intergenic 35.0%
GAGAGAAGAAACATGAAAGT+CGG + chr2_1:70174419-70174438 Msa0221550:CDS 35.0%
TCAAATCTGTGGCAATTGAA+AGG + chr2_1:70174541-70174560 Msa0221550:CDS 35.0%
TTGATTCAAATGCTTTCACC+CGG - chr2_1:70174609-70174628 None:intergenic 35.0%
TCAAAAGAGGACGAATTTGA+AGG + chr2_1:70174625-70174644 Msa0221550:CDS 35.0%
TCAAAACTGTGGCAATTGAA+AGG + chr2_1:70174751-70174770 Msa0221550:CDS 35.0%
GCAATTGAAAGGACAAGTTA+AGG + chr2_1:70174762-70174781 Msa0221550:CDS 35.0%
! AAGAGAAGCAATTTGATAGC+CGG + chr2_1:70174797-70174816 Msa0221550:CDS 35.0%
!! GAAAGCGTTTGAATCAAAAG+AGG + chr2_1:70174822-70174841 Msa0221550:CDS 35.0%
TGAATTTGATGGAGAACTGA+AGG + chr2_1:70174924-70174943 Msa0221550:CDS 35.0%
GGTAAATGATCTTCATCATC+TGG + chr2_1:70175012-70175031 Msa0221550:intron 35.0%
!!! AAGGTTTTTTTTTGGAGAGG+AGG + chr2_1:70175209-70175228 Msa0221550:intron 35.0%
TTCAATACCCTGACAAACTA+TGG + chr2_1:70175248-70175267 Msa0221550:intron 35.0%
AACAGCAATAGTTGCACTAA+AGG - chr2_1:70175288-70175307 None:intergenic 35.0%
GAATTTCAGTTTCGTCACTT+GGG - chr2_1:70175597-70175616 None:intergenic 35.0%
AGAATTTCAGTTTCGTCACT+TGG - chr2_1:70175598-70175617 None:intergenic 35.0%
CTTACACGAGGTTTAATATG+TGG - chr2_1:70175769-70175788 None:intergenic 35.0%
CTACTCCTTGTGTCAATTTA+TGG + chr2_1:70175871-70175890 Msa0221550:CDS 35.0%
CCAATCCATAAATTGACACA+AGG - chr2_1:70175879-70175898 None:intergenic 35.0%
CCTTGTGTCAATTTATGGAT+TGG + chr2_1:70175876-70175895 Msa0221550:CDS 35.0%
ACTTCATCTTCATCGAAAGA+AGG - chr2_1:70175904-70175923 None:intergenic 35.0%
TATGAAGCAAAGACTCTACA+CGG - chr2_1:70176366-70176385 None:intergenic 35.0%
TAACCAAAACTAAGCAGACT+GGG - chr2_1:70176478-70176497 None:intergenic 35.0%
TTAACCAAAACTAAGCAGAC+TGG - chr2_1:70176479-70176498 None:intergenic 35.0%
!! GTTAATTGGAACTGACTTGA+TGG + chr2_1:70176494-70176513 Msa0221550:intron 35.0%
GTTTCTGAACAAAATCTGCA+AGG - chr2_1:70176559-70176578 None:intergenic 35.0%
!! ATCGTTGGTTTGATTCTTGT+TGG - chr2_1:70176638-70176657 None:intergenic 35.0%
! TTCAATGGAGACGATTTTCT+TGG - chr2_1:70176670-70176689 None:intergenic 35.0%
AATCGTCTCCATTGAAATCA+AGG + chr2_1:70176674-70176693 Msa0221550:exon 35.0%
TTAATTTACAACCAACGTGC+AGG - chr2_1:70176752-70176771 None:intergenic 35.0%
! AATATGTGGACTGTGAAATC+AGG + chr2_1:70176770-70176789 Msa0221550:three_prime_UTR 35.0%
TCATTTGCACATTGAGCATT+AGG - chr2_1:70176804-70176823 None:intergenic 35.0%
CAATGTGCAAATGAATTAGC+AGG + chr2_1:70176810-70176829 Msa0221550:three_prime_UTR 35.0%
! ACAATAACAGACATTTTGCG+TGG - chr2_1:70176857-70176876 None:intergenic 35.0%
GAATGAGAAGATTCTCAAAC+AGG + chr2_1:70176883-70176902 Msa0221550:three_prime_UTR 35.0%
! CAGGGTTATCTCAATTTTGT+TGG + chr2_1:70176902-70176921 Msa0221550:three_prime_UTR 35.0%
TACAAGAAAAGGAAAGGACA+CGG - chr2_1:70176929-70176948 None:intergenic 35.0%
! AAGCCAAAGTTTTGCGGTAA+TGG + chr2_1:70172989-70173008 Msa0221550:CDS 40.0%
TCACCATTACCGCAAAACTT+TGG - chr2_1:70172995-70173014 None:intergenic 40.0%
!! TAATGGTGATGATGATGGGA+GGG + chr2_1:70173006-70173025 Msa0221550:CDS 40.0%
ATGATGTTCCTTTGTTACCG+AGG + chr2_1:70173065-70173084 Msa0221550:CDS 40.0%
! TCATTTTCCTCGCTGAAGAA+AGG + chr2_1:70173280-70173299 Msa0221550:CDS 40.0%
TGAGGAAGGCAATAAGAAGA+CGG + chr2_1:70173327-70173346 Msa0221550:CDS 40.0%
GAAGCTCATGAGAAATTGCA+GGG + chr2_1:70173454-70173473 Msa0221550:CDS 40.0%
AAAGAAGCAACTTGAAGGTC+AGG + chr2_1:70173967-70173986 Msa0221550:CDS 40.0%
TCAAGAGAGAAGCAACTTGA+AGG + chr2_1:70174046-70174065 Msa0221550:CDS 40.0%
! GGAGTTTGAATCTAAGATGC+AGG + chr2_1:70174078-70174097 Msa0221550:CDS 40.0%
! TCTAAGATGCAGGAACTTGA+AGG + chr2_1:70174088-70174107 Msa0221550:CDS 40.0%
TCAAAAGAGAAGCAAGTCGA+AGG + chr2_1:70174130-70174149 Msa0221550:CDS 40.0%
GAAGTTTGAAGGCCTAATGA+AGG + chr2_1:70174225-70174244 Msa0221550:CDS 40.0%
TAAACTCCACCTCCTTCATT+AGG - chr2_1:70174240-70174259 None:intergenic 40.0%
GAAGGAGGTGGAGTTTAAAA+AGG + chr2_1:70174243-70174262 Msa0221550:CDS 40.0%
CCTTTACTTGGCATACAAGT+TGG - chr2_1:70174312-70174331 None:intergenic 40.0%
CCAACTTGTATGCCAAGTAA+AGG + chr2_1:70174309-70174328 Msa0221550:CDS 40.0%
GGAGTTTGAATCAAAGCAGA+AGG + chr2_1:70174330-70174349 Msa0221550:CDS 40.0%
GAAACATGAAAGTCGGTTGA+AGG + chr2_1:70174426-70174445 Msa0221550:CDS 40.0%
TAAATTCCAGCTCCTTCACT+TGG - chr2_1:70174483-70174502 None:intergenic 40.0%
GAAGCAATTCGAAAGCCAAA+TGG + chr2_1:70174507-70174526 Msa0221550:CDS 40.0%
GCAATTGAAAGGACAGGTTA+AGG + chr2_1:70174552-70174571 Msa0221550:CDS 40.0%
GCTATCAAACTGCTTCTCTT+TGG - chr2_1:70174587-70174606 None:intergenic 40.0%
! AAGAGAAGCAGTTTGATAGC+CGG + chr2_1:70174587-70174606 Msa0221550:CDS 40.0%
!! GAAGCATTTTGAAAGCCAAG+TGG + chr2_1:70174717-70174736 Msa0221550:CDS 40.0%
! AGAGAAGCAATTTGATAGCC+GGG + chr2_1:70174798-70174817 Msa0221550:CDS 40.0%
TTGATTCAAACGCTTTCACC+CGG - chr2_1:70174819-70174838 None:intergenic 40.0%
AATACCCTGACAAACTATGG+AGG + chr2_1:70175251-70175270 Msa0221550:intron 40.0%
! AATGCCTCCATAGTTTGTCA+GGG - chr2_1:70175258-70175277 None:intergenic 40.0%
! AAATGCCTCCATAGTTTGTC+AGG - chr2_1:70175259-70175278 None:intergenic 40.0%
CTTTGTTCTTACGCAACTTC+TGG + chr2_1:70175318-70175337 Msa0221550:intron 40.0%
AACTTCTGGTGATTCAGTTG+CGG + chr2_1:70175332-70175351 Msa0221550:intron 40.0%
CAATCAACTGCTACCATTGA+TGG + chr2_1:70175553-70175572 Msa0221550:CDS 40.0%
CTGCAAACTTCTTCCATCAA+TGG - chr2_1:70175569-70175588 None:intergenic 40.0%
ATTGCTTCTTCTCTTACACG+AGG - chr2_1:70175781-70175800 None:intergenic 40.0%
AAGATAGCTGTAGAGCTGTT+TGG + chr2_1:70175955-70175974 Msa0221550:CDS 40.0%
!! ATGAAGCACCGACATAGATT+TGG + chr2_1:70176285-70176304 Msa0221550:intron 40.0%
TTTGCACATTGAGCATTAGG+AGG - chr2_1:70176801-70176820 None:intergenic 40.0%
TGCGTGGATCAATAAAGAGA+GGG - chr2_1:70176841-70176860 None:intergenic 40.0%
TTGCGTGGATCAATAAAGAG+AGG - chr2_1:70176842-70176861 None:intergenic 40.0%
! TGCGGTAATGGTGATGATGA+TGG + chr2_1:70173001-70173020 Msa0221550:CDS 45.0%
! GCGGTAATGGTGATGATGAT+GGG + chr2_1:70173002-70173021 Msa0221550:CDS 45.0%
!! GTAATGGTGATGATGATGGG+AGG + chr2_1:70173005-70173024 Msa0221550:CDS 45.0%
CTTTGTTACCGAGGTTTACC+CGG + chr2_1:70173074-70173093 Msa0221550:CDS 45.0%
!! GTTTACCCGGATTTCGAGTT+TGG + chr2_1:70173087-70173106 Msa0221550:CDS 45.0%
GTCCATAAAGCGAGATATCG+AGG + chr2_1:70173372-70173391 Msa0221550:CDS 45.0%
CTCCTCGATATCTCGCTTTA+TGG - chr2_1:70173377-70173396 None:intergenic 45.0%
CGAAGCTCATGAGAAATTGC+AGG + chr2_1:70173453-70173472 Msa0221550:CDS 45.0%
TGATAAGGACCGTGAAAGGA+AGG + chr2_1:70173648-70173667 Msa0221550:CDS 45.0%
GTTCCTCTTCCTTCCTTTCA+CGG - chr2_1:70173660-70173679 None:intergenic 45.0%
AAGGAAGGAAGAGGAACTCA+AGG + chr2_1:70173663-70173682 Msa0221550:CDS 45.0%
TGCATCAAGCTCTCTCTCTT+TGG - chr2_1:70173831-70173850 None:intergenic 45.0%
GCAACTTGAAGGACACATGA+AGG + chr2_1:70174057-70174076 Msa0221550:CDS 45.0%
AAGAGAAGCAAGTCGAAGGA+CGG + chr2_1:70174134-70174153 Msa0221550:CDS 45.0%
GCAACTTAAAGTCCAGGAGA+AGG + chr2_1:70174183-70174202 Msa0221550:CDS 45.0%
GTTTGAAGGCCTAATGAAGG+AGG + chr2_1:70174228-70174247 Msa0221550:CDS 45.0%
AGAGTTTGAAGGCCAAGTGA+AGG + chr2_1:70174468-70174487 Msa0221550:CDS 45.0%
GCAATTCGAAAGCCAAATGG+AGG + chr2_1:70174510-70174529 Msa0221550:CDS 45.0%
TTGATGTGAGCTCCTCCATT+TGG - chr2_1:70174525-70174544 None:intergenic 45.0%
AGGAGCTCACATCAAATCTG+TGG + chr2_1:70174530-70174549 Msa0221550:CDS 45.0%
TCTGTGGCAATTGAAAGGAC+AGG + chr2_1:70174546-70174565 Msa0221550:CDS 45.0%
! AGAGAAGCAGTTTGATAGCC+GGG + chr2_1:70174588-70174607 Msa0221550:CDS 45.0%
TTGATTCATGCTCCTTCGCT+CGG - chr2_1:70174651-70174670 None:intergenic 45.0%
TGAAATTCAAGCGAAGGAGC+TGG + chr2_1:70174684-70174703 Msa0221550:CDS 45.0%
!! GCATTTTGAAAGCCAAGTGG+AGG + chr2_1:70174720-70174739 Msa0221550:CDS 45.0%
AGGAGCTCACATCAAAACTG+TGG + chr2_1:70174740-70174759 Msa0221550:CDS 45.0%
GAAGGAGCTTGAGTTAAGAG+AGG + chr2_1:70174942-70174961 Msa0221550:CDS 45.0%
GAGGAGGACTATGAGTTTCA+AGG + chr2_1:70175225-70175244 Msa0221550:intron 45.0%
! TGAAAATTCGGTGGCAGTTC+TGG + chr2_1:70175843-70175862 Msa0221550:CDS 45.0%
! GAAAATTCGGTGGCAGTTCT+GGG + chr2_1:70175844-70175863 Msa0221550:CDS 45.0%
! TGTAGAGCTGTTTGGAACTC+TGG + chr2_1:70175963-70175982 Msa0221550:CDS 45.0%
! GTAGAGCTGTTTGGAACTCT+GGG + chr2_1:70175964-70175983 Msa0221550:CDS 45.0%
! GGTTTTGCCGATAAAGCTTC+TGG + chr2_1:70175985-70176004 Msa0221550:CDS 45.0%
GGCTTCTTACACTTGATGCT+TGG + chr2_1:70176200-70176219 Msa0221550:intron 45.0%
CACGGACACCAAATCTATGT+CGG - chr2_1:70176296-70176315 None:intergenic 45.0%
ACACTGACGCATGTCTGAAA+CGG - chr2_1:70176343-70176362 None:intergenic 45.0%
TCTTGGATGCAAGAGATCGT+TGG - chr2_1:70176653-70176672 None:intergenic 45.0%
CACATTGAGCATTAGGAGGA+TGG - chr2_1:70176797-70176816 None:intergenic 45.0%
CGGGTAAACCTCGGTAACAA+AGG - chr2_1:70173076-70173095 None:intergenic 50.0%
CTCGAAATCCGGGTAAACCT+CGG - chr2_1:70173085-70173104 None:intergenic 50.0%
GGAAGGGCAGCTCAATTTGA+TGG + chr2_1:70173513-70173532 Msa0221550:CDS 50.0%
GCAACTTGAAGGTCAGGTCA+AGG + chr2_1:70173973-70173992 Msa0221550:CDS 50.0%
GAAGCAATGTGATGAACGGC+TGG + chr2_1:70174012-70174031 Msa0221550:CDS 50.0%
AGAGAAGCAAGTCGAAGGAC+GGG + chr2_1:70174135-70174154 Msa0221550:CDS 50.0%
TGAAGGCCTAATGAAGGAGG+TGG + chr2_1:70174231-70174250 Msa0221550:CDS 50.0%
TTGATGTGAGCTCCTCCACT+TGG - chr2_1:70174735-70174754 None:intergenic 50.0%
! GGAGTTTGAAGCGAAGCAGT+TGG + chr2_1:70174867-70174886 Msa0221550:CDS 50.0%
! GCAGTTGGAAGTGCAACTGA+AGG + chr2_1:70174882-70174901 Msa0221550:CDS 50.0%
! TTGGTGTCCGTGTAGAGTCT+CGG + chr2_1:70176304-70176323 Msa0221550:intron 50.0%
! ACTGACGCATGTCTGAAACG+GGG - chr2_1:70176341-70176360 None:intergenic 50.0%
! CACTGACGCATGTCTGAAAC+GGG - chr2_1:70176342-70176361 None:intergenic 50.0%
! CAGCCCAGTCTGCTTAGTTT+TGG + chr2_1:70176472-70176491 Msa0221550:intron 50.0%
ACGTGCAGGTGCATGAAGAT+GGG - chr2_1:70176738-70176757 None:intergenic 50.0%
AACGTGCAGGTGCATGAAGA+TGG - chr2_1:70176739-70176758 None:intergenic 50.0%
ATCTTCATGCACCTGCACGT+TGG + chr2_1:70176738-70176757 Msa0221550:three_prime_UTR 50.0%
TGCAAGGCCTTTCTTCAGCG+AGG - chr2_1:70173290-70173309 None:intergenic 55.0%
GGACCGTGAAAGGAAGGAAG+AGG + chr2_1:70173654-70173673 Msa0221550:CDS 55.0%
GCAATGTGATGAACGGCTGG+AGG + chr2_1:70174015-70174034 Msa0221550:CDS 55.0%
!! TTCAAAAGCCGGCTCGAGTC+AGG + chr2_1:70174397-70174416 Msa0221550:CDS 55.0%
! TCTTCTCTCCTGACTCGAGC+CGG - chr2_1:70174408-70174427 None:intergenic 55.0%
TGAAGGCCAAGTGAAGGAGC+TGG + chr2_1:70174474-70174493 Msa0221550:CDS 55.0%
CGAATTTGAAGGCCGAGCGA+AGG + chr2_1:70174636-70174655 Msa0221550:CDS 55.0%
!!! GCTTTTCGGGTTTGCTGCTC+AGG + chr2_1:70175930-70175949 Msa0221550:CDS 55.0%
TGGTGTCCGTGTAGAGTCTC+GGG + chr2_1:70176305-70176324 Msa0221550:intron 55.0%
TCCGTGTAGAGTCTCGGGTA+AGG + chr2_1:70176310-70176329 Msa0221550:intron 55.0%
GCCTTACCCGAGACTCTACA+CGG - chr2_1:70176314-70176333 None:intergenic 55.0%
GCAAGTCGAAGGACGGGCGA+TGG + chr2_1:70174141-70174160 Msa0221550:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 70172968 70176993 70172968 ID=Msa0221550;Name=Msa0221550
chr2_1 mRNA 70172968 70176993 70172968 ID=Msa0221550-mRNA-1;Parent=Msa0221550;Name=Msa0221550-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.33|0.25|1|1|1|4|305|833
chr2_1 exon 70172968 70173684 70172968 ID=Msa0221550-mRNA-1:exon:19993;Parent=Msa0221550-mRNA-1
chr2_1 exon 70173827 70175012 70173827 ID=Msa0221550-mRNA-1:exon:19994;Parent=Msa0221550-mRNA-1
chr2_1 exon 70175533 70176006 70175533 ID=Msa0221550-mRNA-1:exon:19995;Parent=Msa0221550-mRNA-1
chr2_1 exon 70176564 70176993 70176564 ID=Msa0221550-mRNA-1:exon:19996;Parent=Msa0221550-mRNA-1
chr2_1 CDS 70172968 70173684 70172968 ID=Msa0221550-mRNA-1:cds;Parent=Msa0221550-mRNA-1
chr2_1 CDS 70173827 70175012 70173827 ID=Msa0221550-mRNA-1:cds;Parent=Msa0221550-mRNA-1
chr2_1 CDS 70175533 70176006 70175533 ID=Msa0221550-mRNA-1:cds;Parent=Msa0221550-mRNA-1
chr2_1 CDS 70176564 70176688 70176564 ID=Msa0221550-mRNA-1:cds;Parent=Msa0221550-mRNA-1
chr2_1 three_prime_UTR 70176689 70176993 70176689 ID=Msa0221550-mRNA-1:three_prime_utr;Parent=Msa0221550-mRNA-1
Gene Sequence

>Msa0221550

ATGAATTTTGAGGCTTTTGATAAGCCAAAGTTTTGCGGTAATGGTGATGATGATGGGAGGGATCATGTTTCTTGTGAATACTTTGAATCTGAAGATGATGATGTTCCTTTGTTACCGAGGTTTACCCGGATTTCGAGTTTGGTAAAACGTGATGTTTCTGTTAAGAAGAGGGATTTTGAGTTTGATGACAACAATTCCTTGCCTATTTCTGCTAAGAAATCAAAGGCGTCACTATATCATGATCACGACAAAGATGAAGATGATATCCCGTTGTTAGATTTAATTAAAAGGCCAAGCAAATCAACTGATAAATCATTTTCCTCGCTGAAGAAAGGCCTTGCATTGTTAGAAAGCTCATTTGAGGAAGGCAATAAGAAGACGGAATTAGAAGAGAATAGATTGCAGTCCATAAAGCGAGATATCGAGGAGTGTCGCAAAGAGCTTGAAAATAAGAAAAAGGAAATTAGTTGTTTTAGAGGAATTGACGAAGCTCATGAGAAATTGCAGGGAAAAATTGAAGAATGTGTTAAGGATTTTGTAGTGAAGGAAGGGCAGCTCAATTTGATGGATGACTTGATTGGAGAGCGCAAGCTAGAGCTCAAGACAAAAGAGATAGAACTTAATCAAGTCAAGGGCAACATTTCAAAAGAAATAGAACTTCGTCAAGTCATTGATAATATTGATAAGGACCGTGAAAGGAAGGAAGAGGAACTCAAGGCCAAAGAGAGAGAGCTTGATGCAATGAACAAATTAATTAGTGGGAAAGCAGAAAAATTAGAGTCCAAAAGGAAGAAGTTGCTAAAGCTAACATCAGAAAAGGAGAATGTTCGTGCTCAAATAAAGGAATTTGAGTCAATAAAGAAGCAACTTGAAGGTCAGGTCAAGGAACTTGAGTCAAAAGAGAAGCAATGTGATGAACGGCTGGAGGTGTTGATATCAAGAGAGAAGCAACTTGAAGGACACATGAAGGAGTTTGAATCTAAGATGCAGGAACTTGAAGGTCGTACGATGCAGCTTGAATCAAAAGAGAAGCAAGTCGAAGGACGGGCGATGGAGCTAAAGTTAAAAGAAATGCAACTTAAAGTCCAGGAGAAGGAGTTTAAATCAAAAGAGGAGAAGTTTGAAGGCCTAATGAAGGAGGTGGAGTTTAAAAAGGAGCACTTTGAAAACCAATCGAATGTGTTAGAGTCAATAGATAACCAACTTGTATGCCAAGTAAAGGAGTTTGAATCAAAGCAGAAGGAATTTGAATGCCAAAAGAAGGAGTCGATAGTAAGACAAAAATATTTCAAAAGCCGGCTCGAGTCAGGAGAGAAGAAACATGAAAGTCGGTTGAAGGAGCATGAATCAAAAGAAAGAGAGTTTGAAGGCCAAGTGAAGGAGCTGGAATTTAAAAAGAAGCAATTCGAAAGCCAAATGGAGGAGCTCACATCAAATCTGTGGCAATTGAAAGGACAGGTTAAGGAACTTGAGTCCAAAGAGAAGCAGTTTGATAGCCGGGTGAAAGCATTTGAATCAAAAGAGGACGAATTTGAAGGCCGAGCGAAGGAGCATGAATCAAAAGAGAGAGAATTTGAAATTCAAGCGAAGGAGCTGGAATCTAAAAAGAAGCATTTTGAAAGCCAAGTGGAGGAGCTCACATCAAAACTGTGGCAATTGAAAGGACAAGTTAAGGAACTTGAGTACAAAGAGAAGCAATTTGATAGCCGGGTGAAAGCGTTTGAATCAAAAGAGGTTGAATTTAAAGATCGAGTGAGGGAGTTTGAAGCGAAGCAGTTGGAAGTGCAACTGAAGGAGCTAAAGTCAATGCAGAATGAATTTGATGGAGAACTGAAGGAGCTTGAGTTAAGAGAGGATCAATATGAAGCATTACTAAAATCATTTGACGAGGAAATTAAATCAGTTACATGTTACACAGATGATCAATCAACTGCTACCATTGATGGAAGAAGTTTGCAGTTGCTCCCAAGTGACGAAACTGAAATTCTAGATAATCTGCAAGGATTATCAGATCCATCAAAAGTTGTTTTGGATATAATACAGAATCCCATTATTCAAAAGTATAAGATGGGAGACAATGCTGTAATTATTGATGATAGAGACATTCTTCTGCTCAAACAACTGATGAGAATCAAACCACATATTAAACCTCGTGTAAGAGAAGAAGCAATGAAGCTAGCACTTAATTTGAAATCCAACATAAGTGAAAATACTGAAAATTCGGTGGCAGTTCTGGGTTTTCTACTCCTTGTGTCAATTTATGGATTGGCTCCTTCTTTCGATGAAGATGAAGTTTTAAAGCTTTTCGGGTTTGCTGCTCAGGACAAGATAGCTGTAGAGCTGTTTGGAACTCTGGGTTTTGCCGATAAAGCTTCTGATTTTGTTCAGAAACTTATCATGAAGAAACAAAATATTGAAGCTGTTAGATTCATTCGTGCTTATATAATGCCAACAAGAATCAAACCAACGATCTCTTGCATCCAAGAAAATCGTCTCCATTGA
Protein sequence

>Msa0221550

MNFEAFDKPKFCGNGDDDGRDHVSCEYFESEDDDVPLLPRFTRISSLVKRDVSVKKRDFEFDDNNSLPISAKKSKASLYHDHDKDEDDIPLLDLIKRPSKSTDKSFSSLKKGLALLESSFEEGNKKTELEENRLQSIKRDIEECRKELENKKKEISCFRGIDEAHEKLQGKIEECVKDFVVKEGQLNLMDDLIGERKLELKTKEIELNQVKGNISKEIELRQVIDNIDKDRERKEEELKAKERELDAMNKLISGKAEKLESKRKKLLKLTSEKENVRAQIKEFESIKKQLEGQVKELESKEKQCDERLEVLISREKQLEGHMKEFESKMQELEGRTMQLESKEKQVEGRAMELKLKEMQLKVQEKEFKSKEEKFEGLMKEVEFKKEHFENQSNVLESIDNQLVCQVKEFESKQKEFECQKKESIVRQKYFKSRLESGEKKHESRLKEHESKEREFEGQVKELEFKKKQFESQMEELTSNLWQLKGQVKELESKEKQFDSRVKAFESKEDEFEGRAKEHESKEREFEIQAKELESKKKHFESQVEELTSKLWQLKGQVKELEYKEKQFDSRVKAFESKEVEFKDRVREFEAKQLEVQLKELKSMQNEFDGELKELELREDQYEALLKSFDEEIKSVTCYTDDQSTATIDGRSLQLLPSDETEILDNLQGLSDPSKVVLDIIQNPIIQKYKMGDNAVIIDDRDILLLKQLMRIKPHIKPRVREEAMKLALNLKSNISENTENSVAVLGFLLLVSIYGLAPSFDEDEVLKLFGFAAQDKIAVELFGTLGFADKASDFVQKLIMKKQNIEAVRFIRAYIMPTRIKPTISCIQENRLH*