AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300690 MtrunA17_Chr2g0325861 91.097 483 26 2 1 466 337 819 0.0 807
Msa0300690 MtrunA17_Chr2g0325861 34.125 337 156 7 28 308 197 523 2.60e-24 106
Msa0300690 MtrunA17_Chr2g0325231 48.506 569 187 6 1 466 304 869 8.97e-132 402
Msa0300690 MtrunA17_Chr2g0325231 42.628 312 132 11 18 293 154 454 1.55e-33 134
Msa0300690 MtrunA17_Chr2g0325231 37.500 232 91 6 137 324 215 436 3.41e-18 87.8
Msa0300690 MtrunA17_Chr2g0325231 33.603 247 127 9 90 305 107 347 1.87e-11 66.6
Msa0300690 MtrunA17_Chr2g0280141 50.622 482 204 8 1 466 252 715 7.35e-122 375
Msa0300690 MtrunA17_Chr2g0325711 46.693 499 190 7 1 466 279 734 7.59e-115 356
Msa0300690 MtrunA17_Chr2g0325711 30.094 319 154 7 50 305 136 448 2.61e-14 75.5
Msa0300690 MtrunA17_Chr2g0325361 51.491 503 201 7 1 466 478 974 4.38e-113 358
Msa0300690 MtrunA17_Chr2g0325361 45.845 349 138 4 1 305 334 675 1.59e-60 214
Msa0300690 MtrunA17_Chr2g0325361 45.317 331 158 4 1 308 264 594 6.00e-52 189
Msa0300690 MtrunA17_Chr2g0325361 37.201 293 165 5 23 302 204 490 8.00e-26 111
Msa0300690 MtrunA17_Chr2g0325811 44.055 513 226 9 5 462 226 732 3.40e-106 334
Msa0300690 MtrunA17_Chr2g0325811 35.321 218 114 2 1 205 365 568 1.59e-21 98.2
Msa0300690 MtrunA17_Chr2g0325321 47.195 517 212 7 1 467 254 759 2.87e-101 319
Msa0300690 MtrunA17_Chr2g0325321 34.921 252 106 3 115 308 245 496 1.26e-17 85.9
Msa0300690 MtrunA17_Chr2g0325321 29.971 347 146 10 40 302 101 434 2.91e-13 72.4
Msa0300690 MtrunA17_Chr2g0325661 48.940 519 198 8 1 466 269 773 4.56e-100 319
Msa0300690 MtrunA17_Chr2g0325661 41.743 218 104 5 87 302 225 421 1.82e-21 97.8
Msa0300690 MtrunA17_Chr6g0471251 43.089 492 220 8 5 466 203 664 1.62e-85 278
Msa0300690 MtrunA17_Chr6g0471251 36.214 243 123 3 1 229 279 503 3.08e-18 87.8
Msa0300690 MtrunA17_Chr2g0325701 43.659 481 207 11 9 470 158 593 4.10e-84 270
Msa0300690 MtrunA17_Chr2g0325701 36.932 176 86 2 1 176 261 411 7.64e-12 67.4
Msa0300690 MtrunA17_Chr5g0416271 48.538 342 166 4 129 468 151 484 1.55e-83 266
Msa0300690 MtrunA17_Chr5g0416271 42.667 150 83 1 1 147 173 322 2.01e-14 75.5
Msa0300690 MtrunA17_Chr2g0325611 48.413 378 157 8 109 466 128 487 5.05e-77 251
Msa0300690 MtrunA17_Chr2g0325611 41.489 188 98 3 1 178 125 310 4.14e-15 77.8
Msa0300690 MtrunA17_Chr2g0325611 35.484 155 61 2 2 156 201 316 3.50e-12 68.6
Msa0300690 MtrunA17_Chr2g0325351 48.128 374 156 6 115 467 230 586 5.16e-77 254
Msa0300690 MtrunA17_Chr2g0325351 39.252 214 102 3 6 219 230 415 1.56e-20 94.7
Msa0300690 MtrunA17_Chr2g0325621 50.298 336 158 3 140 466 223 558 8.10e-71 237
Msa0300690 MtrunA17_Chr2g0325621 34.409 186 98 3 17 178 202 387 1.27e-11 67.0
Msa0300690 MtrunA17_Chr2g0325621 37.333 150 83 1 1 150 249 387 4.20e-11 65.5
Msa0300690 MtrunA17_Chr2g0333341 43.275 342 178 4 139 465 267 607 8.21e-65 220
Msa0300690 MtrunA17_Chr2g0333341 39.344 183 78 5 9 172 281 449 7.82e-13 70.9
Msa0300690 MtrunA17_Chr2g0332961 47.213 305 139 6 139 439 67 353 5.08e-58 195
Msa0300690 MtrunA17_Chr2g0325291 70.270 148 36 2 327 466 1 148 3.62e-56 186
Msa0300690 MtrunA17_Chr2g0325691 47.164 335 154 5 1 313 390 723 3.33e-54 193
Msa0300690 MtrunA17_Chr2g0325691 47.403 308 136 4 1 288 306 607 3.77e-51 184
Msa0300690 MtrunA17_Chr2g0325691 40.000 380 149 6 1 302 250 628 1.01e-45 169
Msa0300690 MtrunA17_Chr2g0325691 42.222 360 142 8 2 312 226 568 1.38e-40 154
Msa0300690 MtrunA17_Chr2g0325691 40.407 344 150 9 2 308 139 464 4.92e-37 144
Msa0300690 MtrunA17_Chr2g0325761 44.444 342 136 6 1 302 264 591 8.74e-53 189
Msa0300690 MtrunA17_Chr2g0325761 42.069 290 142 4 44 307 265 554 3.21e-34 136
Msa0300690 MtrunA17_Chr2g0325761 37.698 252 120 6 87 308 220 464 8.88e-20 92.4
Msa0300690 MtrunA17_Chr2g0325241 44.300 307 148 5 106 391 120 424 3.12e-52 182
Msa0300690 MtrunA17_Chr2g0325241 36.555 238 117 4 2 227 121 336 6.24e-21 94.7
Msa0300690 MtrunA17_Chr2g0325241 38.824 170 93 3 1 159 169 338 1.67e-14 75.5
Msa0300690 MtrunA17_Chr3g0140591 60.241 166 57 2 313 470 9 173 3.67e-51 171
Msa0300690 MtrunA17_Chr2g0332951 50.442 226 84 7 253 468 2 209 1.25e-50 171
Msa0300690 MtrunA17_Chr2g0325771 61.714 175 63 2 295 467 1 173 2.66e-49 166
Msa0300690 MtrunA17_Chr2g0325281 41.765 340 147 6 1 305 254 577 3.70e-48 174
Msa0300690 MtrunA17_Chr2g0325281 42.478 226 113 2 1 223 380 591 3.63e-29 120
Msa0300690 MtrunA17_Chr2g0325281 35.021 237 118 4 137 353 229 449 1.52e-20 94.7
Msa0300690 MtrunA17_Chr5g0434761 57.143 161 61 1 314 466 2 162 9.99e-48 165
Msa0300690 MtrunA17_Chr2g0325641 41.694 307 139 4 57 323 1 307 2.03e-46 164
Msa0300690 MtrunA17_Chr2g0325641 38.720 328 154 6 1 302 1 307 7.71e-37 138
Msa0300690 MtrunA17_Chr2g0325641 39.506 162 88 2 2 153 149 310 4.70e-12 67.0
Msa0300690 MtrunA17_Chr2g0325721 64.162 173 59 1 1 170 329 501 7.27e-38 144
Msa0300690 MtrunA17_Chr2g0325721 40.566 212 110 2 113 308 290 501 2.51e-22 99.8
Msa0300690 MtrunA17_Chr2g0325721 39.600 250 143 3 29 271 242 490 1.19e-21 97.8
Msa0300690 MtrunA17_Chr2g0325721 32.881 295 146 6 28 308 189 445 3.98e-17 84.0
Msa0300690 MtrunA17_Chr2g0325331 55.921 152 59 1 315 458 3 154 1.97e-37 135
Msa0300690 MtrunA17_Chr2g0325631 56.522 161 62 1 314 466 2 162 6.74e-36 134
Msa0300690 MtrunA17_Chr7g0235901 54.839 186 63 3 84 248 126 311 3.36e-35 133
Msa0300690 MtrunA17_Chr7g0235901 46.629 178 84 1 1 178 145 311 1.34e-25 106
Msa0300690 MtrunA17_Chr7g0235901 43.434 198 88 4 131 318 112 295 8.89e-24 101
Msa0300690 MtrunA17_Chr7g0235901 41.975 162 92 1 129 288 150 311 2.17e-21 94.7
Msa0300690 MtrunA17_Chr7g0235901 35.685 241 122 9 90 308 18 247 1.09e-11 65.9
Msa0300690 MtrunA17_Chr2g0325221 37.901 343 160 6 15 314 267 599 6.26e-34 135
Msa0300690 MtrunA17_Chr2g0325221 39.568 278 136 5 2 253 321 592 2.73e-32 130
Msa0300690 MtrunA17_Chr8g0361651 36.076 316 157 8 157 466 26 302 1.93e-33 132
Msa0300690 MtrunA17_Chr4g0044221 36.789 299 151 12 126 406 18 296 2.41e-30 120
Msa0300690 MtrunA17_Chr5g0434751 40.637 251 111 4 113 325 4 254 7.64e-30 118
Msa0300690 MtrunA17_Chr5g0434751 41.065 263 115 4 74 296 4 266 7.06e-29 115
Msa0300690 MtrunA17_Chr5g0434751 39.100 289 116 5 1 247 1 271 4.79e-26 107
Msa0300690 MtrunA17_Chr5g0434751 44.853 136 63 2 180 308 1 131 1.63e-14 73.9
Msa0300690 MtrunA17_Chr2g0325651 49.383 162 71 2 115 265 227 388 3.28e-26 109
Msa0300690 MtrunA17_Chr2g0325651 46.296 162 74 3 1 159 236 387 7.66e-23 100
Msa0300690 MtrunA17_Chr2g0325651 40.964 166 89 3 141 306 215 371 2.62e-19 89.7
Msa0300690 MtrunA17_Chr2g0325751 47.368 133 63 1 122 254 5 130 3.50e-21 90.1
Msa0300690 MtrunA17_Chr2g0325751 41.615 161 57 4 162 313 3 135 6.38e-18 80.9
Msa0300690 MtrunA17_Chr4g0024061 29.577 497 248 15 17 466 108 549 1.29e-20 95.1
Msa0300690 MtrunA17_Chr4g0024061 24.318 440 255 10 24 440 29 413 2.55e-11 66.2
Msa0300690 MtrunA17_Chr2g0325781 45.977 174 74 8 9 171 138 302 8.75e-19 87.0
Msa0300690 MtrunA17_Chr2g0325781 50.847 118 40 2 131 240 194 301 3.07e-18 85.5
Msa0300690 MtrunA17_Chr2g0325781 32.932 249 133 9 55 294 78 301 7.84e-13 69.3
Msa0300690 MtrunA17_Chr2g0325781 48.352 91 43 1 129 215 214 304 5.34e-11 63.5
Msa0300690 MtrunA17_Chr7g0235921 67.857 84 26 1 383 466 1 83 1.50e-18 81.3
Msa0300690 MtrunA17_Chr2g0325211 37.349 166 84 3 161 310 2 163 7.45e-17 78.6
Msa0300690 MtrunA17_Chr2g0325791 43.357 143 60 3 90 225 41 169 4.22e-16 79.3
Msa0300690 MtrunA17_Chr2g0325791 38.272 162 89 3 97 254 2 156 5.37e-16 79.0
Msa0300690 MtrunA17_Chr2g0325261 45.370 108 45 1 115 222 213 306 4.69e-13 69.7
Msa0300690 MtrunA17_Chr2g0325341 45.370 108 45 1 115 222 213 306 4.69e-13 69.7
Msa0300690 MtrunA17_Chr2g0325731 63.636 55 20 0 113 167 4 58 2.98e-12 62.0
Msa0300690 MtrunA17_Chr2g0325201 41.463 123 69 1 112 234 109 228 8.92e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300690 AT5G27220.1 28.773 497 295 14 3 470 341 807 1.33e-16 83.6
Msa0300690 AT5G27220.3 28.773 497 295 14 3 470 341 807 1.33e-16 83.6
Msa0300690 AT5G27220.4 28.195 493 303 13 3 470 341 807 1.81e-16 83.2
Msa0300690 AT5G27220.2 28.195 493 303 13 3 470 341 807 1.81e-16 83.2

Find 142 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACTCCTTGTGTCAATTTA+TGG 0.080642 2_3:+56202731 Msa0300690:CDS
CTCCTCAATATCTCGCTTTA+TGG 0.092724 2_3:-56200106 None:intergenic
TGAAAATTCGGTGGCAGTTC+TGG 0.150498 2_3:+56202703 Msa0300690:CDS
CTTTAGCAACTTCTTCCTTT+TGG 0.223344 2_3:-56200523 None:intergenic
TTTGATTTCTTAACAGAAAT+AGG 0.239890 2_3:-56199901 None:intergenic
CGTTGTTAGATTTAATTAAA+AGG 0.247437 2_3:+56199968 Msa0300690:five_prime_UTR
TGCATCAAGCTCTCTCTCTT+TGG 0.269901 2_3:-56200460 None:intergenic
AGATCCATCAAAAGTTGTTT+TGG 0.291459 2_3:+56202499 Msa0300690:CDS
GAAGGAGGTGGAATTTAAAA+AGG 0.305809 2_3:+56200874 Msa0300690:CDS
TTAATATCGACTCCTTCTTT+TGG 0.309523 2_3:-56200994 None:intergenic
TGAATTTGCTTTGGAACTTA+AGG 0.312804 2_3:+56200437 Msa0300690:five_prime_UTR
GTTTACCCGGATTTCGAGTT+TGG 0.322265 2_3:+56199819 Msa0300690:five_prime_UTR
AGAGCTTGAAAATAAGAAAA+AGG 0.324967 2_3:+56200137 Msa0300690:five_prime_UTR
TGTAGAGCTGTTTGGAACTC+TGG 0.329670 2_3:+56202823 Msa0300690:CDS
CAATCAACTGCTACCATTGA+TGG 0.335095 2_3:+56202413 Msa0300690:CDS
GTTCCTCTTCCTTCCTTTCA+CGG 0.342021 2_3:-56200389 None:intergenic
TAACATACCAGAAGCTTTAT+CGG 0.346500 2_3:-56202852 None:intergenic
GCTATCAAACTGCTTCTCTT+TGG 0.353127 2_3:-56201425 None:intergenic
CCTTGTGTCAATTTATGGAT+TGG 0.361905 2_3:+56202736 Msa0300690:CDS
TTGATCCATGCTCCTTCGCT+TGG 0.387670 2_3:-56201489 None:intergenic
AAGTGAAAATACTGAAAATT+CGG 0.388056 2_3:+56202691 Msa0300690:CDS
GAAAATTCGGTGGCAGTTCT+GGG 0.395040 2_3:+56202704 Msa0300690:CDS
GAATGTTCGTGCTCAAATAA+AGG 0.401392 2_3:+56200563 Msa0300690:five_prime_UTR
TAAATTCCACCTCCTTCATT+AGG 0.402615 2_3:-56200868 None:intergenic
AGAATTTCAGTTTCGTCACT+TGG 0.405145 2_3:-56202455 None:intergenic
TTTGACGAGGAAATTAAATC+AGG 0.407950 2_3:+56201832 Msa0300690:CDS
TTGACTCTAACACATTCAAT+TGG 0.421070 2_3:-56200910 None:intergenic
GGAAGGGCAGCTCTATTTGA+TGG 0.424280 2_3:+56200245 Msa0300690:five_prime_UTR
TTGATTTAAACTCCTTCTCC+TGG 0.429349 2_3:-56200826 None:intergenic
TATTTGATGGATGACTTGAT+TGG 0.430087 2_3:+56200258 Msa0300690:five_prime_UTR
AAGATAGCTGTAGAGCTGTT+TGG 0.433585 2_3:+56202815 Msa0300690:CDS
GTAGAGCTGTTTGGAACTCT+GGG 0.438058 2_3:+56202824 Msa0300690:CDS
TCAACGCAGAATGAATTTGA+TGG 0.438153 2_3:+56201754 Msa0300690:CDS
TGCGGTAATGGTGATGATGA+TGG 0.443280 2_3:+56199733 Msa0300690:five_prime_UTR
TAGATTCCAGCTCCTTCACT+TGG 0.444751 2_3:-56201111 None:intergenic
CGAAGCTCATGAGAAATTGC+AGG 0.452129 2_3:+56200185 Msa0300690:five_prime_UTR
TTGATTCAAACTCCTTTACT+TGG 0.452256 2_3:-56200952 None:intergenic
CTTACACGAGGTTTAATATG+TGG 0.453106 2_3:-56202626 None:intergenic
GATTTATCAGTTGATTTGCT+TGG 0.454214 2_3:-56199991 None:intergenic
TTGAATTTAAAGATCGAGTG+AGG 0.457501 2_3:+56201686 Msa0300690:CDS
CGTTGTTACAGCACCAAGCT+TGG 0.457929 2_3:+56199393 Msa0300690:five_prime_UTR
TCAAGAGAGAAGCAACTTCA+AGG 0.461907 2_3:+56200677 Msa0300690:five_prime_UTR
TCACCATTACCGCAAAACTT+TGG 0.464980 2_3:-56199724 None:intergenic
GGAGTTTGAAGTGAAGCAGT+TGG 0.474398 2_3:+56201708 Msa0300690:CDS
TTGATGTGAGCTCCTCCACT+TGG 0.478027 2_3:-56201573 None:intergenic
GCTAAAGCTAACATCAGAAA+AGG 0.480977 2_3:+56200539 Msa0300690:five_prime_UTR
GCAATTGAAAGGACAAGTTA+AGG 0.483479 2_3:+56201603 Msa0300690:CDS
GAAGGAGTTTAAATCAAAAG+AGG 0.484213 2_3:+56200832 Msa0300690:CDS
AGAGAAGCAATGTGATGAAC+GGG 0.485753 2_3:+56200640 Msa0300690:five_prime_UTR
GTCCATAAAGCGAGATATTG+AGG 0.485999 2_3:+56200104 Msa0300690:five_prime_UTR
CCTTTACTTGGCATACAAGT+TGG 0.488568 2_3:-56200940 None:intergenic
CTTTGTTACCGAGGTTTACC+CGG 0.489728 2_3:+56199806 Msa0300690:five_prime_UTR
TTGATGTGAGCTCCTCCACT+TGG 0.490871 2_3:-56201153 None:intergenic
CATTATTCAAAAGTATAAGA+TGG 0.494090 2_3:+56202538 Msa0300690:CDS
CAATGAACAAATTAATTAGC+GGG 0.494366 2_3:+56200481 Msa0300690:five_prime_UTR
TGATGTTTCTGTTAAGAAGA+GGG 0.496115 2_3:+56199849 Msa0300690:five_prime_UTR
AGTCATTGATAATATTGATA+AGG 0.496791 2_3:+56200365 Msa0300690:five_prime_UTR
TGAAATTCAAGCGAAGGAGC+TGG 0.502421 2_3:+56201525 Msa0300690:CDS
TCTAAGATGCAGGAACTTGA+AGG 0.507887 2_3:+56200719 Msa0300690:CDS
CTGCAAACTTCTTCCATCAA+TGG 0.511498 2_3:-56202426 None:intergenic
AAGAGAAGCAATTTGATAGC+CGG 0.511794 2_3:+56201638 Msa0300690:CDS
TCAAAAGAAAGAAAGTTTGA+AGG 0.512316 2_3:+56201088 Msa0300690:CDS
TACAAGTTACGATCCAAGCT+TGG 0.515145 2_3:-56199406 None:intergenic
TCTTCTCTTCTGACTCGAGC+CGG 0.516034 2_3:-56201036 None:intergenic
GAAACATGAAAGTCGGTTGA+AGG 0.517920 2_3:+56201057 Msa0300690:CDS
AGAGAAGCAGTTTGATAGCC+GGG 0.518876 2_3:+56201429 Msa0300690:CDS
GGAATTTGAATGCCAAAAGA+AGG 0.520295 2_3:+56200982 Msa0300690:CDS
AGAATTTGAAATTCAAGCGA+AGG 0.522189 2_3:+56201519 Msa0300690:CDS
TCAAAAGAGGAGAAGTTTGA+AGG 0.522468 2_3:+56200845 Msa0300690:CDS
AGAGAAGCAATTTGATAGCC+GGG 0.528136 2_3:+56201639 Msa0300690:CDS
GAATTTCAGTTTCGTCACTT+GGG 0.529088 2_3:-56202454 None:intergenic
CCAACTTGTATGCCAAGTAA+AGG 0.529486 2_3:+56200940 Msa0300690:CDS
TTGATTCAAACGCTTTCACC+CGG 0.529873 2_3:-56201657 None:intergenic
AGAAATGCAACTTAAAGTCC+AGG 0.531894 2_3:+56200808 Msa0300690:CDS
ATTGTTAGAAAGCTCATTTG+AGG 0.533517 2_3:+56200041 Msa0300690:five_prime_UTR
GGAGTTTGAATCTAAGATGC+AGG 0.536802 2_3:+56200709 Msa0300690:CDS
AGAGAAGCAAGTCGAAGGAC+GGG 0.541425 2_3:+56200766 Msa0300690:CDS
AAGAGAAGCAGTTTGATAGC+CGG 0.545775 2_3:+56201428 Msa0300690:CDS
TCAAAACTGTGGCAATTGAA+AGG 0.545936 2_3:+56201592 Msa0300690:CDS
ACTTCATCTTCATCGAAAGA+AGG 0.546038 2_3:-56202761 None:intergenic
GAAGTTTGAAGGCCTAATGA+AGG 0.546338 2_3:+56200856 Msa0300690:CDS
GCAAGTCGAAGGACGGGCGA+TGG 0.547167 2_3:+56200772 Msa0300690:CDS
GCGGTAATGGTGATGATGAT+GGG 0.548173 2_3:+56199734 Msa0300690:five_prime_UTR
ATTATTCAAAAGTATAAGAT+GGG 0.548310 2_3:+56202539 Msa0300690:CDS
TCAAAAGAGGACGAATTTGA+AGG 0.549498 2_3:+56201466 Msa0300690:CDS
CTCGAAATCCGGGTAAACCT+CGG 0.550108 2_3:-56199814 None:intergenic
TGAATTTGATGGAGAACTGA+AGG 0.552236 2_3:+56201765 Msa0300690:CDS
GAAGGCCAAGCGAAGGAGCA+TGG 0.555210 2_3:+56201484 Msa0300690:CDS
GTGATGTTTCTGTTAAGAAG+AGG 0.556042 2_3:+56199848 Msa0300690:five_prime_UTR
TCAAATCTGTGGCAATTGAA+AGG 0.559650 2_3:+56201172 Msa0300690:CDS
TGATAAGGACCGTGAAAGGA+AGG 0.560715 2_3:+56200380 Msa0300690:five_prime_UTR
TTAGAAAGCTCATTTGAGGA+AGG 0.561900 2_3:+56200045 Msa0300690:five_prime_UTR
GATAGAACTTAATCAAGTCA+AGG 0.567093 2_3:+56200311 Msa0300690:five_prime_UTR
TTAACCAAAACTAAGCAGAC+TGG 0.570521 2_3:-56203316 None:intergenic
GAAAGCATTTGAATCAAAAG+AGG 0.571886 2_3:+56201453 Msa0300690:CDS
AAAGTTTGAAGGCCAAGTGA+AGG 0.572538 2_3:+56201099 Msa0300690:CDS
AAGAGAAGCAATGTGATGAA+CGG 0.574776 2_3:+56200639 Msa0300690:five_prime_UTR
GAAAGCGTTTGAATCAAAAG+AGG 0.576259 2_3:+56201663 Msa0300690:CDS
AAGAGAAGCAAGTCGAAGGA+CGG 0.580246 2_3:+56200765 Msa0300690:CDS
TGAAGGCCAAGTGAAGGAGC+TGG 0.581942 2_3:+56201105 Msa0300690:CDS
ATGAGCTTTCTAACAATGCA+AGG 0.582002 2_3:-56200035 None:intergenic
GGAGTTTGAATCAAAGCAGA+AGG 0.582316 2_3:+56200961 Msa0300690:CDS
TTGATTCAAATGCTTTCACC+CGG 0.583537 2_3:-56201447 None:intergenic
GGACCGTGAAAGGAAGGAAG+AGG 0.585661 2_3:+56200386 Msa0300690:five_prime_UTR
TTTCTTAACAGAAATAGGCA+AGG 0.588441 2_3:-56199896 None:intergenic
TGAAGGCCTAATGAAGGAGG+TGG 0.589996 2_3:+56200862 Msa0300690:CDS
CGAATTTGAAGGCCAAGCGA+AGG 0.593921 2_3:+56201477 Msa0300690:CDS
AAGAGAAGAAACATGAAAGT+CGG 0.597099 2_3:+56201050 Msa0300690:CDS
ATAGAACTTAATCAAGTCAA+GGG 0.597599 2_3:+56200312 Msa0300690:five_prime_UTR
TGAATTTAAAGATCGAGTGA+GGG 0.599120 2_3:+56201687 Msa0300690:CDS
TCAAAAGAGAAGCAAGTCGA+AGG 0.600747 2_3:+56200761 Msa0300690:CDS
ATGATGTTCCTTTGTTACCG+AGG 0.602142 2_3:+56199797 Msa0300690:five_prime_UTR
CGGGTAAACCTCGGTAACAA+AGG 0.604343 2_3:-56199805 None:intergenic
TAACCAAAACTAAGCAGACT+GGG 0.605017 2_3:-56203315 None:intergenic
GCAACTTAAAGTCCAGGAGA+AGG 0.605338 2_3:+56200814 Msa0300690:CDS
GCAACTTCAAGGACACATGA+AGG 0.605750 2_3:+56200688 Msa0300690:exon
GCAACTTGAAGGTCAGGTCA+AGG 0.608454 2_3:+56200604 Msa0300690:five_prime_UTR
GCAATGTGATGAACGGGTGG+AGG 0.609089 2_3:+56200646 Msa0300690:five_prime_UTR
GCAATGAACAAATTAATTAG+CGG 0.612107 2_3:+56200480 Msa0300690:five_prime_UTR
CAAGCTAGAGCTCAAGACAA+AGG 0.612367 2_3:+56200287 Msa0300690:five_prime_UTR
TGAGGAAGGCAATAAGAAGA+CGG 0.615201 2_3:+56200059 Msa0300690:five_prime_UTR
TGAAAATACTGAAAATTCGG+TGG 0.615980 2_3:+56202694 Msa0300690:CDS
GCAGTTGGAAGTGCAACTGA+AGG 0.616833 2_3:+56201723 Msa0300690:CDS
ATATTGATAAGGACCGTGAA+AGG 0.619247 2_3:+56200376 Msa0300690:five_prime_UTR
GCAATTTGAAAGCCAAGTGG+AGG 0.623150 2_3:+56201141 Msa0300690:CDS
TCTGTGGCAATTGAAAGGAC+AGG 0.625484 2_3:+56201177 Msa0300690:CDS
TATTTCTGTTAAGAAATCAA+AGG 0.625575 2_3:+56199903 Msa0300690:five_prime_UTR
GAAATTCTAGATAATCTGCA+AGG 0.625701 2_3:+56202470 Msa0300690:CDS
AAGGAAGGAAGAGGAACTCA+AGG 0.628919 2_3:+56200395 Msa0300690:five_prime_UTR
GAAGCTCATGAGAAATTGCA+GGG 0.629107 2_3:+56200186 Msa0300690:five_prime_UTR
GAAGGAGCTTGAGTTAAGAG+AGG 0.633737 2_3:+56201783 Msa0300690:CDS
TGCAAGGCCTTTCTTCAGCG+AGG 0.638330 2_3:-56200019 None:intergenic
TAATGGTGATGATGATGGGA+GGG 0.651761 2_3:+56199738 Msa0300690:five_prime_UTR
CCAATCCATAAATTGACACA+AGG 0.656897 2_3:-56202736 None:intergenic
GAAGCAATGTGATGAACGGG+TGG 0.657837 2_3:+56200643 Msa0300690:five_prime_UTR
ATTGCTTCTTCTCTTACACG+AGG 0.664142 2_3:-56202638 None:intergenic
AGGAGCTCACATCAAATCTG+TGG 0.671175 2_3:+56201161 Msa0300690:CDS
GTTTGAAGGCCTAATGAAGG+AGG 0.671285 2_3:+56200859 Msa0300690:CDS
GTAATGGTGATGATGATGGG+AGG 0.675360 2_3:+56199737 Msa0300690:five_prime_UTR
ATTACTAAAATCATTTGACG+AGG 0.688803 2_3:+56201819 Msa0300690:CDS
GAAGCAATTTGAAAGCCAAG+TGG 0.692664 2_3:+56201138 Msa0300690:CDS
AGGAGCTCACATCAAAACTG+TGG 0.719251 2_3:+56201581 Msa0300690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTAATTAAATCTAACAA+CGG - chr2_3:56199970-56199989 None:intergenic 15.0%
!! AAAAATTGAAGAATGTATTA+AGG + chr2_3:56200209-56200228 Msa0300690:five_prime_UTR 15.0%
!! ATTAAAAGATAAAGTTGAAA+TGG - chr2_3:56201894-56201913 None:intergenic 15.0%
!!! ACTTTATCTTTTAATATCTT+AGG + chr2_3:56201899-56201918 Msa0300690:intron 15.0%
!! ACACATATATTTAAATGTTT+GGG - chr2_3:56202248-56202267 None:intergenic 15.0%
!!! ATCTTATACTTTTGAATAAT+GGG - chr2_3:56202539-56202558 None:intergenic 15.0%
!!! ATTATTCAAAAGTATAAGAT+GGG + chr2_3:56202539-56202558 Msa0300690:CDS 15.0%
!! AAGAAAATAAATATAATTGC+TGG - chr2_3:56202903-56202922 None:intergenic 15.0%
!!! GAAGTTTTTAGAATATCTTT+TGG - chr2_3:56199335-56199354 None:intergenic 20.0%
!! TGTTCAATTGAAAAATTTCT+AGG - chr2_3:56199625-56199644 None:intergenic 20.0%
!!! TTTTTTTGTTTTAATTTCCC+AGG + chr2_3:56199657-56199676 Msa0300690:intron 20.0%
!!! TTTGATTTCTTAACAGAAAT+AGG - chr2_3:56199904-56199923 None:intergenic 20.0%
!!! TATTTCTGTTAAGAAATCAA+AGG + chr2_3:56199903-56199922 Msa0300690:five_prime_UTR 20.0%
!! CGTTGTTAGATTTAATTAAA+AGG + chr2_3:56199968-56199987 Msa0300690:five_prime_UTR 20.0%
!!! AGTCATTGATAATATTGATA+AGG + chr2_3:56200365-56200384 Msa0300690:five_prime_UTR 20.0%
!! CACACATATATTTAAATGTT+TGG - chr2_3:56202249-56202268 None:intergenic 20.0%
!!! TACTGATGTTTCTATAAATA+TGG + chr2_3:56202347-56202366 Msa0300690:intron 20.0%
!!! ACTGATGTTTCTATAAATAT+GGG + chr2_3:56202348-56202367 Msa0300690:intron 20.0%
!!! CATCTTATACTTTTGAATAA+TGG - chr2_3:56202540-56202559 None:intergenic 20.0%
!!! CATTATTCAAAAGTATAAGA+TGG + chr2_3:56202538-56202557 Msa0300690:CDS 20.0%
!! AAGTGAAAATACTGAAAATT+CGG + chr2_3:56202691-56202710 Msa0300690:CDS 20.0%
!! ATGATTAAGATATTATCTGT+TGG + chr2_3:56203012-56203031 Msa0300690:intron 20.0%
!!! TCTATTTCTTAAAATGTTTG+AGG + chr2_3:56203252-56203271 Msa0300690:intron 20.0%
! ACAATAAACAAGTACAATGA+CGG - chr2_3:56199550-56199569 None:intergenic 25.0%
!! TTATTGTTTCATTTTGCTTC+TGG + chr2_3:56199563-56199582 Msa0300690:intron 25.0%
!! TACTTGAATGATGAATTTTG+AGG + chr2_3:56199690-56199709 Msa0300690:five_prime_UTR 25.0%
! AGAGCTTGAAAATAAGAAAA+AGG + chr2_3:56200137-56200156 Msa0300690:five_prime_UTR 25.0%
!!! AAGGAAATTAGTTGTTTTAG+AGG + chr2_3:56200156-56200175 Msa0300690:five_prime_UTR 25.0%
!! TATTAAGGATTTTGTAGTGA+AGG + chr2_3:56200224-56200243 Msa0300690:five_prime_UTR 25.0%
! ATAGAACTTAATCAAGTCAA+GGG + chr2_3:56200312-56200331 Msa0300690:five_prime_UTR 25.0%
!!! AGCAAATTCAGCAATTTTTT+GGG - chr2_3:56200427-56200446 None:intergenic 25.0%
!!! AAGCAAATTCAGCAATTTTT+TGG - chr2_3:56200428-56200447 None:intergenic 25.0%
!! AAAAATTGCTGAATTTGCTT+TGG + chr2_3:56200428-56200447 Msa0300690:five_prime_UTR 25.0%
! GCAATGAACAAATTAATTAG+CGG + chr2_3:56200480-56200499 Msa0300690:five_prime_UTR 25.0%
! CAATGAACAAATTAATTAGC+GGG + chr2_3:56200481-56200500 Msa0300690:five_prime_UTR 25.0%
! TCAAAAGAAAGAAAGTTTGA+AGG + chr2_3:56201088-56201107 Msa0300690:CDS 25.0%
! ATTACTAAAATCATTTGACG+AGG + chr2_3:56201819-56201838 Msa0300690:CDS 25.0%
!!! AGGTTTTATTCTTATCGAAT+CGG + chr2_3:56201919-56201938 Msa0300690:intron 25.0%
!!! TGAGTTTCAAGGTTTTTTTT+TGG + chr2_3:56202042-56202061 Msa0300690:intron 25.0%
! ATAGAAACATCAGTAAATCA+AGG - chr2_3:56202342-56202361 None:intergenic 25.0%
!! ATATCCAAAACAACTTTTGA+TGG - chr2_3:56202506-56202525 None:intergenic 25.0%
!! TTCAGTATTTTCACTTATGT+TGG - chr2_3:56202687-56202706 None:intergenic 25.0%
!!! GATGAAGTTTTAAAGCTTTT+CGG + chr2_3:56202776-56202795 Msa0300690:CDS 25.0%
!!! ATGAAGTTTTAAAGCTTTTC+GGG + chr2_3:56202777-56202796 Msa0300690:CDS 25.0%
! ATATATCATGTAGAGTCTAT+AGG - chr2_3:56202979-56202998 None:intergenic 25.0%
! TTAAGATATTATCTGTTGGT+AGG + chr2_3:56203016-56203035 Msa0300690:intron 25.0%
ACAACGCTAAAAGAGAAAAA+CGG - chr2_3:56199379-56199398 None:intergenic 30.0%
CATGAAGTAATGCTACATTA+CGG - chr2_3:56199591-56199610 None:intergenic 30.0%
TCAAGTAATTTACACTTCCT+GGG - chr2_3:56199677-56199696 None:intergenic 30.0%
TTCAAGTAATTTACACTTCC+TGG - chr2_3:56199678-56199697 None:intergenic 30.0%
! TTTGATAAGCCAAAGTTTTG+CGG + chr2_3:56199715-56199734 Msa0300690:five_prime_UTR 30.0%
! ATTTTACCAAACTCGAAATC+CGG - chr2_3:56199828-56199847 None:intergenic 30.0%
! TGATGTTTCTGTTAAGAAGA+GGG + chr2_3:56199849-56199868 Msa0300690:five_prime_UTR 30.0%
TTTCTTAACAGAAATAGGCA+AGG - chr2_3:56199899-56199918 None:intergenic 30.0%
!! GATTTATCAGTTGATTTGCT+TGG - chr2_3:56199994-56200013 None:intergenic 30.0%
ATTGTTAGAAAGCTCATTTG+AGG + chr2_3:56200041-56200060 Msa0300690:five_prime_UTR 30.0%
! TATTTGATGGATGACTTGAT+TGG + chr2_3:56200258-56200277 Msa0300690:five_prime_UTR 30.0%
GATAGAACTTAATCAAGTCA+AGG + chr2_3:56200311-56200330 Msa0300690:five_prime_UTR 30.0%
!! TGAATTTGCTTTGGAACTTA+AGG + chr2_3:56200437-56200456 Msa0300690:five_prime_UTR 30.0%
CAGAAAAATTAGAGTCCAAA+AGG + chr2_3:56200508-56200527 Msa0300690:five_prime_UTR 30.0%
GTCAATAAAAAGCAACTTGA+AGG + chr2_3:56200593-56200612 Msa0300690:five_prime_UTR 30.0%
GAAGGAGTTTAAATCAAAAG+AGG + chr2_3:56200832-56200851 Msa0300690:CDS 30.0%
TTGACTCTAACACATTCAAT+TGG - chr2_3:56200913-56200932 None:intergenic 30.0%
TTGATTCAAACTCCTTTACT+TGG - chr2_3:56200955-56200974 None:intergenic 30.0%
!!! TTAATATCGACTCCTTCTTT+TGG - chr2_3:56200997-56201016 None:intergenic 30.0%
GACAAAAATATTTCGAAAGC+CGG + chr2_3:56201017-56201036 Msa0300690:CDS 30.0%
AAGAGAAGAAACATGAAAGT+CGG + chr2_3:56201050-56201069 Msa0300690:CDS 30.0%
!! GAAAGCATTTGAATCAAAAG+AGG + chr2_3:56201243-56201262 Msa0300690:intron 30.0%
TCAAAAGAGGATGAATTTGA+AGG + chr2_3:56201256-56201275 Msa0300690:intron 30.0%
! AGAATTTGAAATTCAAGCGA+AGG + chr2_3:56201309-56201328 Msa0300690:intron 30.0%
!! GAAAGCATTTGAATCAAAAG+AGG + chr2_3:56201243-56201262 Msa0300690:intron 30.0%
! AGAATTTGAAATTCAAGCGA+AGG + chr2_3:56201309-56201328 Msa0300690:intron 30.0%
TTGAATTTAAAGATCGAGTG+AGG + chr2_3:56201686-56201705 Msa0300690:CDS 30.0%
TGAATTTAAAGATCGAGTGA+GGG + chr2_3:56201687-56201706 Msa0300690:CDS 30.0%
TTTGACGAGGAAATTAAATC+AGG + chr2_3:56201832-56201851 Msa0300690:CDS 30.0%
TATGATATGACTGAGTTTCA+AGG + chr2_3:56202031-56202050 Msa0300690:intron 30.0%
!!! TTCAAGGTTTTTTTTTGGAG+AGG + chr2_3:56202047-56202066 Msa0300690:intron 30.0%
TGCTGTTATTGTCTATTCTT+TGG + chr2_3:56202142-56202161 Msa0300690:intron 30.0%
! TCAGTTGATACTAACAGTAA+CGG - chr2_3:56202216-56202235 None:intergenic 30.0%
GAAATTCTAGATAATCTGCA+AGG + chr2_3:56202470-56202489 Msa0300690:CDS 30.0%
! AGATCCATCAAAAGTTGTTT+TGG + chr2_3:56202499-56202518 Msa0300690:CDS 30.0%
TGAAAATACTGAAAATTCGG+TGG + chr2_3:56202694-56202713 Msa0300690:CDS 30.0%
! TAACATACCAGAAGCTTTAT+CGG - chr2_3:56202855-56202874 None:intergenic 30.0%
AAGCTGAACTGATATGTAAT+TGG + chr2_3:56203039-56203058 Msa0300690:intron 30.0%
AGAGAAAAACGGAAGATGAA+AGG - chr2_3:56199368-56199387 None:intergenic 35.0%
TGACGGTGTAAATTAGTTAC+AGG - chr2_3:56199533-56199552 None:intergenic 35.0%
! TTTTACCAAACTCGAAATCC+GGG - chr2_3:56199827-56199846 None:intergenic 35.0%
! GTGATGTTTCTGTTAAGAAG+AGG + chr2_3:56199848-56199867 Msa0300690:five_prime_UTR 35.0%
ATGAGCTTTCTAACAATGCA+AGG - chr2_3:56200038-56200057 None:intergenic 35.0%
TTAGAAAGCTCATTTGAGGA+AGG + chr2_3:56200045-56200064 Msa0300690:five_prime_UTR 35.0%
! AAGGATTTTGTAGTGAAGGA+AGG + chr2_3:56200228-56200247 Msa0300690:five_prime_UTR 35.0%
! AGGATTTTGTAGTGAAGGAA+GGG + chr2_3:56200229-56200248 Msa0300690:five_prime_UTR 35.0%
ATATTGATAAGGACCGTGAA+AGG + chr2_3:56200376-56200395 Msa0300690:five_prime_UTR 35.0%
! CTTTAGCAACTTCTTCCTTT+TGG - chr2_3:56200526-56200545 None:intergenic 35.0%
GCTAAAGCTAACATCAGAAA+AGG + chr2_3:56200539-56200558 Msa0300690:five_prime_UTR 35.0%
GAATGTTCGTGCTCAAATAA+AGG + chr2_3:56200563-56200582 Msa0300690:five_prime_UTR 35.0%
TAAAAAGCAACTTGAAGGTC+AGG + chr2_3:56200598-56200617 Msa0300690:five_prime_UTR 35.0%
AAGAGAAGCAATGTGATGAA+CGG + chr2_3:56200639-56200658 Msa0300690:five_prime_UTR 35.0%
AGAAATGCAACTTAAAGTCC+AGG + chr2_3:56200808-56200827 Msa0300690:CDS 35.0%
TTGATTTAAACTCCTTCTCC+TGG - chr2_3:56200829-56200848 None:intergenic 35.0%
TCAAAAGAGGAGAAGTTTGA+AGG + chr2_3:56200845-56200864 Msa0300690:CDS 35.0%
TAAATTCCACCTCCTTCATT+AGG - chr2_3:56200871-56200890 None:intergenic 35.0%
GAAGGAGGTGGAATTTAAAA+AGG + chr2_3:56200874-56200893 Msa0300690:CDS 35.0%
GGAATTTGAATGCCAAAAGA+AGG + chr2_3:56200982-56201001 Msa0300690:CDS 35.0%
TCAAATCTGTGGCAATTGAA+AGG + chr2_3:56201172-56201191 Msa0300690:CDS 35.0%
GATATCAAACTGCTTCTCTT+TGG - chr2_3:56201218-56201237 None:intergenic 35.0%
AAGAGAAGCAGTTTGATATC+CGG + chr2_3:56201218-56201237 Msa0300690:intron 35.0%
TTGATTCAAATGCTTTCACC+CGG - chr2_3:56201240-56201259 None:intergenic 35.0%
TCAAATCTGTGGCAATTGAA+AGG + chr2_3:56201172-56201191 Msa0300690:CDS 35.0%
TTGATTCAAATGCTTTCACC+CGG - chr2_3:56201240-56201259 None:intergenic 35.0%
TCAAAAGAGGACGAATTTGA+AGG + chr2_3:56201466-56201485 Msa0300690:CDS 35.0%
TCAAAACTGTGGCAATTGAA+AGG + chr2_3:56201592-56201611 Msa0300690:CDS 35.0%
GCAATTGAAAGGACAAGTTA+AGG + chr2_3:56201603-56201622 Msa0300690:CDS 35.0%
! AAGAGAAGCAATTTGATAGC+CGG + chr2_3:56201638-56201657 Msa0300690:CDS 35.0%
!! GAAAGCGTTTGAATCAAAAG+AGG + chr2_3:56201663-56201682 Msa0300690:CDS 35.0%
TCAACGCAGAATGAATTTGA+TGG + chr2_3:56201754-56201773 Msa0300690:CDS 35.0%
TGAATTTGATGGAGAACTGA+AGG + chr2_3:56201765-56201784 Msa0300690:CDS 35.0%
GGTAAATGATCTTCATCATC+TGG + chr2_3:56201853-56201872 Msa0300690:intron 35.0%
!!! AAGGTTTTTTTTTGGAGAGG+AGG + chr2_3:56202050-56202069 Msa0300690:intron 35.0%
TTCAATACCCTGACAAACTA+TGG + chr2_3:56202089-56202108 Msa0300690:intron 35.0%
GAATTTCAGTTTCGTCACTT+GGG - chr2_3:56202457-56202476 None:intergenic 35.0%
AGAATTTCAGTTTCGTCACT+TGG - chr2_3:56202458-56202477 None:intergenic 35.0%
CTTACACGAGGTTTAATATG+TGG - chr2_3:56202629-56202648 None:intergenic 35.0%
CTACTCCTTGTGTCAATTTA+TGG + chr2_3:56202731-56202750 Msa0300690:CDS 35.0%
CCAATCCATAAATTGACACA+AGG - chr2_3:56202739-56202758 None:intergenic 35.0%
CCTTGTGTCAATTTATGGAT+TGG + chr2_3:56202736-56202755 Msa0300690:CDS 35.0%
ACTTCATCTTCATCGAAAGA+AGG - chr2_3:56202764-56202783 None:intergenic 35.0%
TACAAGTTACGATCCAAGCT+TGG - chr2_3:56199409-56199428 None:intergenic 40.0%
! AAGCCAAAGTTTTGCGGTAA+TGG + chr2_3:56199721-56199740 Msa0300690:five_prime_UTR 40.0%
TCACCATTACCGCAAAACTT+TGG - chr2_3:56199727-56199746 None:intergenic 40.0%
!! TAATGGTGATGATGATGGGA+GGG + chr2_3:56199738-56199757 Msa0300690:five_prime_UTR 40.0%
ATGATGTTCCTTTGTTACCG+AGG + chr2_3:56199797-56199816 Msa0300690:five_prime_UTR 40.0%
! TCATTTTCCTCGCTGAAGAA+AGG + chr2_3:56200012-56200031 Msa0300690:five_prime_UTR 40.0%
TGAGGAAGGCAATAAGAAGA+CGG + chr2_3:56200059-56200078 Msa0300690:five_prime_UTR 40.0%
GTCCATAAAGCGAGATATTG+AGG + chr2_3:56200104-56200123 Msa0300690:five_prime_UTR 40.0%
CTCCTCAATATCTCGCTTTA+TGG - chr2_3:56200109-56200128 None:intergenic 40.0%
GAAGCTCATGAGAAATTGCA+GGG + chr2_3:56200186-56200205 Msa0300690:five_prime_UTR 40.0%
AGAGAAGCAATGTGATGAAC+GGG + chr2_3:56200640-56200659 Msa0300690:five_prime_UTR 40.0%
TCAAGAGAGAAGCAACTTCA+AGG + chr2_3:56200677-56200696 Msa0300690:five_prime_UTR 40.0%
! GGAGTTTGAATCTAAGATGC+AGG + chr2_3:56200709-56200728 Msa0300690:CDS 40.0%
! TCTAAGATGCAGGAACTTGA+AGG + chr2_3:56200719-56200738 Msa0300690:CDS 40.0%
TCAAAAGAGAAGCAAGTCGA+AGG + chr2_3:56200761-56200780 Msa0300690:CDS 40.0%
GAAGTTTGAAGGCCTAATGA+AGG + chr2_3:56200856-56200875 Msa0300690:CDS 40.0%
CCTTTACTTGGCATACAAGT+TGG - chr2_3:56200943-56200962 None:intergenic 40.0%
CCAACTTGTATGCCAAGTAA+AGG + chr2_3:56200940-56200959 Msa0300690:CDS 40.0%
GGAGTTTGAATCAAAGCAGA+AGG + chr2_3:56200961-56200980 Msa0300690:CDS 40.0%
GAAACATGAAAGTCGGTTGA+AGG + chr2_3:56201057-56201076 Msa0300690:CDS 40.0%
AAAGTTTGAAGGCCAAGTGA+AGG + chr2_3:56201099-56201118 Msa0300690:CDS 40.0%
GAAGCAATTTGAAAGCCAAG+TGG + chr2_3:56201138-56201157 Msa0300690:CDS 40.0%
GCAATTGAAAGGACAGGTTA+AGG + chr2_3:56201183-56201202 Msa0300690:intron 40.0%
AGAGAAGCAGTTTGATATCC+GGG + chr2_3:56201219-56201238 Msa0300690:intron 40.0%
GCAATTGAAAGGACAGGTTA+AGG + chr2_3:56201183-56201202 Msa0300690:intron 40.0%
GCTATCAAACTGCTTCTCTT+TGG - chr2_3:56201428-56201447 None:intergenic 40.0%
! AAGAGAAGCAGTTTGATAGC+CGG + chr2_3:56201428-56201447 Msa0300690:CDS 40.0%
!! GAAGCATTTTGAAAGCCAAG+TGG + chr2_3:56201558-56201577 Msa0300690:CDS 40.0%
! AGAGAAGCAATTTGATAGCC+GGG + chr2_3:56201639-56201658 Msa0300690:CDS 40.0%
TTGATTCAAACGCTTTCACC+CGG - chr2_3:56201660-56201679 None:intergenic 40.0%
AATACCCTGACAAACTATGG+AGG + chr2_3:56202092-56202111 Msa0300690:intron 40.0%
! AATGCCTCCATAGTTTGTCA+GGG - chr2_3:56202099-56202118 None:intergenic 40.0%
! AAATGCCTCCATAGTTTGTC+AGG - chr2_3:56202100-56202119 None:intergenic 40.0%
AACAGCAACAGTTGCACTAA+AGG - chr2_3:56202129-56202148 None:intergenic 40.0%
AACTTCTGGTGATTCAGTTG+CGG + chr2_3:56202173-56202192 Msa0300690:intron 40.0%
CAATCAACTGCTACCATTGA+TGG + chr2_3:56202413-56202432 Msa0300690:CDS 40.0%
CTGCAAACTTCTTCCATCAA+TGG - chr2_3:56202429-56202448 None:intergenic 40.0%
ATTGCTTCTTCTCTTACACG+AGG - chr2_3:56202641-56202660 None:intergenic 40.0%
AAGATAGCTGTAGAGCTGTT+TGG + chr2_3:56202815-56202834 Msa0300690:CDS 40.0%
!! ATGAAGCACCGACATAGATT+TGG + chr2_3:56203125-56203144 Msa0300690:intron 40.0%
! TGCGGTAATGGTGATGATGA+TGG + chr2_3:56199733-56199752 Msa0300690:five_prime_UTR 45.0%
! GCGGTAATGGTGATGATGAT+GGG + chr2_3:56199734-56199753 Msa0300690:five_prime_UTR 45.0%
!! GTAATGGTGATGATGATGGG+AGG + chr2_3:56199737-56199756 Msa0300690:five_prime_UTR 45.0%
CTTTGTTACCGAGGTTTACC+CGG + chr2_3:56199806-56199825 Msa0300690:five_prime_UTR 45.0%
!! GTTTACCCGGATTTCGAGTT+TGG + chr2_3:56199819-56199838 Msa0300690:five_prime_UTR 45.0%
CGAAGCTCATGAGAAATTGC+AGG + chr2_3:56200185-56200204 Msa0300690:five_prime_UTR 45.0%
CAAGCTAGAGCTCAAGACAA+AGG + chr2_3:56200287-56200306 Msa0300690:five_prime_UTR 45.0%
TGATAAGGACCGTGAAAGGA+AGG + chr2_3:56200380-56200399 Msa0300690:five_prime_UTR 45.0%
GTTCCTCTTCCTTCCTTTCA+CGG - chr2_3:56200392-56200411 None:intergenic 45.0%
AAGGAAGGAAGAGGAACTCA+AGG + chr2_3:56200395-56200414 Msa0300690:five_prime_UTR 45.0%
TGCATCAAGCTCTCTCTCTT+TGG - chr2_3:56200463-56200482 None:intergenic 45.0%
GCAACTTCAAGGACACATGA+AGG + chr2_3:56200688-56200707 Msa0300690:exon 45.0%
AAGAGAAGCAAGTCGAAGGA+CGG + chr2_3:56200765-56200784 Msa0300690:CDS 45.0%
GCAACTTAAAGTCCAGGAGA+AGG + chr2_3:56200814-56200833 Msa0300690:CDS 45.0%
GTTTGAAGGCCTAATGAAGG+AGG + chr2_3:56200859-56200878 Msa0300690:CDS 45.0%
TAGATTCCAGCTCCTTCACT+TGG - chr2_3:56201114-56201133 None:intergenic 45.0%
GCAATTTGAAAGCCAAGTGG+AGG + chr2_3:56201141-56201160 Msa0300690:CDS 45.0%
AGGAGCTCACATCAAATCTG+TGG + chr2_3:56201161-56201180 Msa0300690:CDS 45.0%
TCTGTGGCAATTGAAAGGAC+AGG + chr2_3:56201177-56201196 Msa0300690:CDS 45.0%
TTGATTCATGCTCCTTCGCT+CGG - chr2_3:56201282-56201301 None:intergenic 45.0%
TGAAATTCAAGCGAAGGAGC+TGG + chr2_3:56201315-56201334 Msa0300690:intron 45.0%
GAAGCATGTTGAAAGCCAAG+TGG + chr2_3:56201348-56201367 Msa0300690:intron 45.0%
AGGAGCTCACATCAAATCTG+TGG + chr2_3:56201161-56201180 Msa0300690:CDS 45.0%
TCTGTGGCAATTGAAAGGAC+AGG + chr2_3:56201177-56201196 Msa0300690:CDS 45.0%
! AGAGAAGCAGTTTGATAGCC+GGG + chr2_3:56201429-56201448 Msa0300690:CDS 45.0%
TGAAATTCAAGCGAAGGAGC+TGG + chr2_3:56201315-56201334 Msa0300690:intron 45.0%
!! GCATTTTGAAAGCCAAGTGG+AGG + chr2_3:56201561-56201580 Msa0300690:CDS 45.0%
AGGAGCTCACATCAAAACTG+TGG + chr2_3:56201581-56201600 Msa0300690:CDS 45.0%
! GGAGTTTGAAGTGAAGCAGT+TGG + chr2_3:56201708-56201727 Msa0300690:CDS 45.0%
GAAGGAGCTTGAGTTAAGAG+AGG + chr2_3:56201783-56201802 Msa0300690:CDS 45.0%
GAGGAGGACTATGAGTTTCA+AGG + chr2_3:56202066-56202085 Msa0300690:intron 45.0%
!! CTTTGGTCTTACGCAACTTC+TGG + chr2_3:56202159-56202178 Msa0300690:intron 45.0%
! TGAAAATTCGGTGGCAGTTC+TGG + chr2_3:56202703-56202722 Msa0300690:CDS 45.0%
! GAAAATTCGGTGGCAGTTCT+GGG + chr2_3:56202704-56202723 Msa0300690:CDS 45.0%
! TGTAGAGCTGTTTGGAACTC+TGG + chr2_3:56202823-56202842 Msa0300690:CDS 45.0%
! GTAGAGCTGTTTGGAACTCT+GGG + chr2_3:56202824-56202843 Msa0300690:CDS 45.0%
! GGTTTTGCCGATAAAGCTTC+TGG + chr2_3:56202845-56202864 Msa0300690:CDS 45.0%
GGCTTCTTACACTTGATGCT+TGG + chr2_3:56203060-56203079 Msa0300690:intron 45.0%
CACGGACACCAAATCTATGT+CGG - chr2_3:56203136-56203155 None:intergenic 45.0%
ACACTGACGCATGTCTGAAA+CGG - chr2_3:56203183-56203202 None:intergenic 45.0%
CGTTGTTACAGCACCAAGCT+TGG + chr2_3:56199393-56199412 Msa0300690:five_prime_UTR 50.0%
CGGGTAAACCTCGGTAACAA+AGG - chr2_3:56199808-56199827 None:intergenic 50.0%
CTCGAAATCCGGGTAAACCT+CGG - chr2_3:56199817-56199836 None:intergenic 50.0%
GGAAGGGCAGCTCTATTTGA+TGG + chr2_3:56200245-56200264 Msa0300690:five_prime_UTR 50.0%
GCAACTTGAAGGTCAGGTCA+AGG + chr2_3:56200604-56200623 Msa0300690:five_prime_UTR 50.0%
GAAGCAATGTGATGAACGGG+TGG + chr2_3:56200643-56200662 Msa0300690:five_prime_UTR 50.0%
AGAGAAGCAAGTCGAAGGAC+GGG + chr2_3:56200766-56200785 Msa0300690:CDS 50.0%
TGAAGGCCTAATGAAGGAGG+TGG + chr2_3:56200862-56200881 Msa0300690:CDS 50.0%
! TCTTCTCTTCTGACTCGAGC+CGG - chr2_3:56201039-56201058 None:intergenic 50.0%
TTGATGTGAGCTCCTCCACT+TGG - chr2_3:56201156-56201175 None:intergenic 50.0%
TGAATTTGAAGGCCGAGCGA+AGG + chr2_3:56201267-56201286 Msa0300690:intron 50.0%
GCATGTTGAAAGCCAAGTGG+AGG + chr2_3:56201351-56201370 Msa0300690:intron 50.0%
TTGATGTGAGCTCCTCCACT+TGG - chr2_3:56201156-56201175 None:intergenic 50.0%
CGAATTTGAAGGCCAAGCGA+AGG + chr2_3:56201477-56201496 Msa0300690:CDS 50.0%
TTGATCCATGCTCCTTCGCT+TGG - chr2_3:56201492-56201511 None:intergenic 50.0%
TTGATGTGAGCTCCTCCACT+TGG - chr2_3:56201156-56201175 None:intergenic 50.0%
! GCAGTTGGAAGTGCAACTGA+AGG + chr2_3:56201723-56201742 Msa0300690:CDS 50.0%
! TTGGTGTCCGTGTAGAGTCT+CGG + chr2_3:56203144-56203163 Msa0300690:intron 50.0%
! ACTGACGCATGTCTGAAACG+GGG - chr2_3:56203181-56203200 None:intergenic 50.0%
! CACTGACGCATGTCTGAAAC+GGG - chr2_3:56203182-56203201 None:intergenic 50.0%
TGCAAGGCCTTTCTTCAGCG+AGG - chr2_3:56200022-56200041 None:intergenic 55.0%
GGACCGTGAAAGGAAGGAAG+AGG + chr2_3:56200386-56200405 Msa0300690:five_prime_UTR 55.0%
GCAATGTGATGAACGGGTGG+AGG + chr2_3:56200646-56200665 Msa0300690:five_prime_UTR 55.0%
TGAAGGCCAAGTGAAGGAGC+TGG + chr2_3:56201105-56201124 Msa0300690:CDS 55.0%
!!! GCTTTTCGGGTTTGCTGCTC+AGG + chr2_3:56202790-56202809 Msa0300690:CDS 55.0%
TGGTGTCCGTGTAGAGTCTC+GGG + chr2_3:56203145-56203164 Msa0300690:intron 55.0%
TCCGTGTAGAGTCTCGGGTA+AGG + chr2_3:56203150-56203169 Msa0300690:intron 55.0%
GCCTTACCCGAGACTCTACA+CGG - chr2_3:56203154-56203173 None:intergenic 55.0%
GAAGGCCAAGCGAAGGAGCA+TGG + chr2_3:56201484-56201503 Msa0300690:CDS 60.0%
GCAAGTCGAAGGACGGGCGA+TGG + chr2_3:56200772-56200791 Msa0300690:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 56199325 56203328 56199325 ID=Msa0300690;Name=Msa0300690
chr2_3 mRNA 56199325 56203328 56199325 ID=Msa0300690-mRNA-1;Parent=Msa0300690;Name=Msa0300690-mRNA-1;_AED=0.13;_eAED=0.13;_QI=1126|0.75|0.8|0.8|0.5|0.4|5|0|470
chr2_3 exon 56199325 56199425 56199325 ID=Msa0300690-mRNA-1:exon:18001;Parent=Msa0300690-mRNA-1
chr2_3 exon 56199679 56201198 56199679 ID=Msa0300690-mRNA-1:exon:18002;Parent=Msa0300690-mRNA-1
chr2_3 exon 56201409 56201853 56201409 ID=Msa0300690-mRNA-1:exon:18003;Parent=Msa0300690-mRNA-1
chr2_3 exon 56202408 56202866 56202408 ID=Msa0300690-mRNA-1:exon:18004;Parent=Msa0300690-mRNA-1
chr2_3 exon 56203315 56203328 56203315 ID=Msa0300690-mRNA-1:exon:18005;Parent=Msa0300690-mRNA-1
chr2_3 five_prime_UTR 56199325 56199425 56199325 ID=Msa0300690-mRNA-1:five_prime_utr;Parent=Msa0300690-mRNA-1
chr2_3 five_prime_UTR 56199679 56200703 56199679 ID=Msa0300690-mRNA-1:five_prime_utr;Parent=Msa0300690-mRNA-1
chr2_3 CDS 56200704 56201198 56200704 ID=Msa0300690-mRNA-1:cds;Parent=Msa0300690-mRNA-1
chr2_3 CDS 56201409 56201853 56201409 ID=Msa0300690-mRNA-1:cds;Parent=Msa0300690-mRNA-1
chr2_3 CDS 56202408 56202866 56202408 ID=Msa0300690-mRNA-1:cds;Parent=Msa0300690-mRNA-1
chr2_3 CDS 56203315 56203328 56203315 ID=Msa0300690-mRNA-1:cds;Parent=Msa0300690-mRNA-1
Gene Sequence

>Msa0300690

ATGAAGGAGTTTGAATCTAAGATGCAGGAACTTGAAGGTCGTACGATGCAGCTTGAATCAAAAGAGAAGCAAGTCGAAGGACGGGCGATGGAGCTAAAGTTAAAAGAAATGCAACTTAAAGTCCAGGAGAAGGAGTTTAAATCAAAAGAGGAGAAGTTTGAAGGCCTAATGAAGGAGGTGGAATTTAAAAAGGAGCACTTTGAAAACCAATTGAATGTGTTAGAGTCAATAGATAACCAACTTGTATGCCAAGTAAAGGAGTTTGAATCAAAGCAGAAGGAATTTGAATGCCAAAAGAAGGAGTCGATATTAAGACAAAAATATTTCGAAAGCCGGCTCGAGTCAGAAGAGAAGAAACATGAAAGTCGGTTGAAGGAGCATGAATCAAAAGAAAGAAAGTTTGAAGGCCAAGTGAAGGAGCTGGAATCTAAAAAGAAGCAATTTGAAAGCCAAGTGGAGGAGCTCACATCAAATCTGTGGCAATTGAAAGGACAGGTTAAGGAACTTGAGTCCAAAGAGAAGCAGTTTGATAGCCGGGTGAAAGCATTTGAATCAAAAGAGGACGAATTTGAAGGCCAAGCGAAGGAGCATGGATCAAAAGAGAGAGAATTTGAAATTCAAGCGAAGGAGCTGGAATCTAAAAAGAAGCATTTTGAAAGCCAAGTGGAGGAGCTCACATCAAAACTGTGGCAATTGAAAGGACAAGTTAAGGAACTTGAGTACAAAGAGAAGCAATTTGATAGCCGGGTGAAAGCGTTTGAATCAAAAGAGGTTGAATTTAAAGATCGAGTGAGGGAGTTTGAAGTGAAGCAGTTGGAAGTGCAACTGAAGGAGCTAAAGTCAACGCAGAATGAATTTGATGGAGAACTGAAGGAGCTTGAGTTAAGAGAGGATCAATATGAAGCATTACTAAAATCATTTGACGAGGAAATTAAATCAGATGATCAATCAACTGCTACCATTGATGGAAGAAGTTTGCAGTTGCTCCCAAGTGACGAAACTGAAATTCTAGATAATCTGCAAGGATTATCAGATCCATCAAAAGTTGTTTTGGATATAATACAGAATCCCATTATTCAAAAGTATAAGATGGGAGACAATGCTGTAATTATTGATGATAGAGACATTCTTCTGCTCAAACAACTGATGAGAATCAAACCACATATTAAACCTCGTGTAAGAGAAGAAGCAATGAAGCTAGCACTTAATTTGAAATCCAACATAAGTGAAAATACTGAAAATTCGGTGGCAGTTCTGGGTTTTCTACTCCTTGTGTCAATTTATGGATTGGCTCCTTCTTTCGATGAAGATGAAGTTTTAAAGCTTTTCGGGTTTGCTGCTCAGGACAAGATAGCTGTAGAGCTGTTTGGAACTCTGGGTTTTGCCGATAAAGCTTCTGCCCAGTCTGCTTAG
Protein sequence

>Msa0300690

MKEFESKMQELEGRTMQLESKEKQVEGRAMELKLKEMQLKVQEKEFKSKEEKFEGLMKEVEFKKEHFENQLNVLESIDNQLVCQVKEFESKQKEFECQKKESILRQKYFESRLESEEKKHESRLKEHESKERKFEGQVKELESKKKQFESQVEELTSNLWQLKGQVKELESKEKQFDSRVKAFESKEDEFEGQAKEHGSKEREFEIQAKELESKKKHFESQVEELTSKLWQLKGQVKELEYKEKQFDSRVKAFESKEVEFKDRVREFEVKQLEVQLKELKSTQNEFDGELKELELREDQYEALLKSFDEEIKSDDQSTATIDGRSLQLLPSDETEILDNLQGLSDPSKVVLDIIQNPIIQKYKMGDNAVIIDDRDILLLKQLMRIKPHIKPRVREEAMKLALNLKSNISENTENSVAVLGFLLLVSIYGLAPSFDEDEVLKLFGFAAQDKIAVELFGTLGFADKASAQSA*