AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0338210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338210 MtrunA17_Chr2g0325861 90.289 865 62 3 1 848 1 860 0.0 1474
Msa0338210 MtrunA17_Chr2g0325321 53.374 815 291 9 29 810 3 761 0.0 653
Msa0338210 MtrunA17_Chr2g0325231 47.609 920 358 16 1 827 1 889 0.0 606
Msa0338210 MtrunA17_Chr2g0325711 45.663 830 316 13 36 829 26 756 0.0 561
Msa0338210 MtrunA17_Chr2g0325721 64.510 510 167 5 2 506 1 501 3.61e-179 525
Msa0338210 MtrunA17_Chr2g0325721 35.878 393 192 14 296 644 125 501 2.45e-24 107
Msa0338210 MtrunA17_Chr2g0325281 55.941 606 205 7 29 602 3 578 1.08e-174 518
Msa0338210 MtrunA17_Chr2g0325281 39.474 190 90 2 293 482 420 584 8.03e-16 81.6
Msa0338210 MtrunA17_Chr2g0325281 36.434 129 70 1 528 656 252 368 2.44e-11 67.4
Msa0338210 MtrunA17_Chr2g0325361 52.416 683 245 10 29 641 3 675 2.02e-170 522
Msa0338210 MtrunA17_Chr2g0325361 47.598 666 295 11 217 834 342 1001 2.71e-137 435
Msa0338210 MtrunA17_Chr2g0325661 43.344 909 320 16 2 829 1 795 4.44e-162 494
Msa0338210 MtrunA17_Chr2g0280141 43.975 805 322 16 50 834 47 742 1.83e-161 491
Msa0338210 MtrunA17_Chr2g0325691 42.436 747 306 15 5 652 5 726 4.13e-117 372
Msa0338210 MtrunA17_Chr2g0325221 43.143 649 251 14 2 589 1 592 6.31e-115 365
Msa0338210 MtrunA17_Chr2g0325811 43.866 595 252 10 2 541 1 568 6.49e-107 348
Msa0338210 MtrunA17_Chr2g0325811 42.647 544 239 10 341 829 226 751 2.56e-103 338
Msa0338210 MtrunA17_Chr6g0471251 42.679 560 260 9 296 829 162 686 6.12e-98 322
Msa0338210 MtrunA17_Chr6g0471251 43.011 558 187 13 1 506 1 479 3.70e-81 277
Msa0338210 MtrunA17_Chr2g0325761 38.410 742 258 18 2 663 1 623 1.94e-94 311
Msa0338210 MtrunA17_Chr2g0325621 53.155 412 163 6 26 436 8 390 5.98e-94 309
Msa0338210 MtrunA17_Chr2g0325621 51.233 365 174 2 476 836 223 587 1.26e-81 276
Msa0338210 MtrunA17_Chr2g0325611 49.242 396 171 6 465 845 144 524 3.87e-88 291
Msa0338210 MtrunA17_Chr2g0325611 41.100 309 124 4 196 492 54 316 2.02e-41 160
Msa0338210 MtrunA17_Chr2g0325611 29.070 344 194 10 27 363 10 310 3.49e-17 85.9
Msa0338210 MtrunA17_Chr2g0325351 46.067 445 171 8 1 412 1 409 9.55e-87 290
Msa0338210 MtrunA17_Chr2g0325351 43.943 487 226 8 386 829 125 607 6.16e-85 285
Msa0338210 MtrunA17_Chr2g0325701 42.884 527 222 11 298 807 125 589 3.40e-86 286
Msa0338210 MtrunA17_Chr2g0325701 39.560 455 199 11 5 431 5 411 1.94e-58 210
Msa0338210 MtrunA17_Chr5g0416271 48.468 359 169 5 459 814 145 490 1.64e-83 276
Msa0338210 MtrunA17_Chr5g0416271 39.633 381 169 9 50 421 15 343 5.97e-53 192
Msa0338210 MtrunA17_Chr2g0333341 42.798 486 181 7 2 427 1 449 4.26e-78 265
Msa0338210 MtrunA17_Chr2g0333341 40.343 466 233 11 389 814 154 614 7.20e-68 237
Msa0338210 MtrunA17_Chr2g0325651 38.776 490 175 11 8 495 21 387 2.39e-67 229
Msa0338210 MtrunA17_Chr2g0325651 50.980 153 64 2 460 601 236 388 9.92e-27 113
Msa0338210 MtrunA17_Chr2g0325651 36.328 256 145 5 324 565 136 387 9.26e-21 95.5
Msa0338210 MtrunA17_Chr2g0325651 34.432 273 161 6 383 642 104 371 2.94e-20 94.0
Msa0338210 MtrunA17_Chr2g0325291 70.000 170 43 2 668 829 1 170 2.65e-61 207
Msa0338210 MtrunA17_Chr7g0235901 45.317 331 150 5 72 391 1 311 5.60e-60 206
Msa0338210 MtrunA17_Chr7g0235901 54.839 186 63 3 420 584 126 311 2.36e-33 130
Msa0338210 MtrunA17_Chr7g0235901 43.284 201 90 4 467 657 112 298 2.07e-23 102
Msa0338210 MtrunA17_Chr2g0325781 44.545 330 138 11 2 314 1 302 4.76e-59 203
Msa0338210 MtrunA17_Chr2g0325781 45.402 174 75 8 345 507 138 302 3.36e-18 86.3
Msa0338210 MtrunA17_Chr2g0325781 53.846 104 38 1 473 576 208 301 4.24e-18 86.3
Msa0338210 MtrunA17_Chr2g0325781 49.451 91 42 1 465 551 214 304 1.20e-11 66.6
Msa0338210 MtrunA17_Chr2g0325781 31.707 246 140 8 391 630 78 301 1.98e-11 66.2
Msa0338210 MtrunA17_Chr5g0434741 39.844 384 129 9 2 363 1 304 3.96e-57 198
Msa0338210 MtrunA17_Chr2g0325741 46.835 316 111 11 1 281 1 294 1.60e-56 196
Msa0338210 MtrunA17_Chr2g0332961 48.065 310 134 7 475 780 67 353 1.73e-56 198
Msa0338210 MtrunA17_Chr2g0332961 41.732 127 52 3 61 180 10 121 1.20e-14 76.6
Msa0338210 MtrunA17_Chr2g0332961 38.172 186 89 3 273 457 68 228 1.51e-12 70.1
Msa0338210 MtrunA17_Chr2g0325261 39.418 378 142 6 1 364 1 305 2.35e-56 196
Msa0338210 MtrunA17_Chr2g0325261 47.475 99 38 1 474 572 222 306 2.71e-13 71.6
Msa0338210 MtrunA17_Chr2g0325261 32.743 226 127 5 380 586 87 306 8.18e-12 67.4
Msa0338210 MtrunA17_Chr2g0325341 39.418 378 142 6 1 364 1 305 2.35e-56 196
Msa0338210 MtrunA17_Chr2g0325341 47.475 99 38 1 474 572 222 306 2.71e-13 71.6
Msa0338210 MtrunA17_Chr2g0325341 32.743 226 127 5 380 586 87 306 8.18e-12 67.4
Msa0338210 MtrunA17_Chr5g0434761 57.377 183 70 1 655 829 2 184 2.20e-54 189
Msa0338210 MtrunA17_Chr2g0325241 45.455 297 147 3 449 732 130 424 3.03e-54 194
Msa0338210 MtrunA17_Chr2g0325241 36.869 198 103 2 301 484 119 308 7.11e-20 93.2
Msa0338210 MtrunA17_Chr2g0325241 33.005 203 108 4 244 446 135 309 2.63e-12 69.7
Msa0338210 MtrunA17_Chr3g0140591 60.119 168 58 2 651 810 6 172 8.89e-50 173
Msa0338210 MtrunA17_Chr2g0332951 52.727 220 82 7 596 807 2 207 2.92e-49 173
Msa0338210 MtrunA17_Chr2g0325771 65.217 161 54 1 649 807 12 172 3.91e-48 168
Msa0338210 MtrunA17_Chr2g0325641 45.018 271 130 3 393 644 1 271 9.97e-45 164
Msa0338210 MtrunA17_Chr2g0325641 40.719 334 163 4 258 584 2 307 1.78e-41 155
Msa0338210 MtrunA17_Chr2g0325641 39.385 358 173 5 295 652 1 314 2.68e-38 145
Msa0338210 MtrunA17_Chr2g0325641 34.685 222 121 3 253 460 109 320 5.62e-15 77.0
Msa0338210 MtrunA17_Chr2g0325641 35.714 154 80 1 502 655 1 135 6.90e-12 67.8
Msa0338210 MtrunA17_Chr2g0325201 47.479 238 97 3 153 375 2 226 2.20e-44 160
Msa0338210 MtrunA17_Chr2g0325631 57.377 183 70 1 655 829 2 184 9.39e-44 159
Msa0338210 MtrunA17_Chr8g0361651 36.443 343 168 8 493 829 26 324 1.51e-37 147
Msa0338210 MtrunA17_Chr2g0325331 55.556 153 60 1 656 800 3 155 3.60e-36 134
Msa0338210 MtrunA17_Chr5g0434751 40.673 327 143 6 326 652 4 279 1.64e-30 122
Msa0338210 MtrunA17_Chr5g0434751 39.844 256 115 4 449 665 4 259 3.93e-29 118
Msa0338210 MtrunA17_Chr5g0434751 41.176 289 152 2 295 583 1 271 8.32e-29 117
Msa0338210 MtrunA17_Chr5g0434751 51.049 143 68 1 516 656 1 143 5.03e-21 94.7
Msa0338210 MtrunA17_Chr5g0434751 34.982 283 135 4 252 513 17 271 3.06e-17 83.2
Msa0338210 MtrunA17_Chr4g0024061 29.828 580 291 19 305 829 53 571 5.43e-28 120
Msa0338210 MtrunA17_Chr4g0024061 23.820 445 256 9 360 781 29 413 4.16e-11 67.0
Msa0338210 MtrunA17_Chr4g0044221 52.941 136 44 5 625 747 168 296 1.70e-27 114
Msa0338210 MtrunA17_Chr4g0044221 38.830 188 101 4 131 316 15 190 7.00e-20 91.7
Msa0338210 MtrunA17_Chr2g0325801 42.169 166 56 5 1 166 1 126 1.55e-22 94.0
Msa0338210 MtrunA17_Chr7g0235921 61.538 104 39 1 724 827 1 103 3.04e-22 92.4
Msa0338210 MtrunA17_Chr2g0325751 45.455 154 61 3 498 649 3 135 7.60e-22 92.8
Msa0338210 MtrunA17_Chr2g0325751 46.617 133 64 1 458 590 5 130 8.28e-21 90.1
Msa0338210 MtrunA17_Chr2g0325751 38.554 166 74 2 290 455 2 139 2.08e-15 74.7
Msa0338210 MtrunA17_Chr3g0140581 34.911 169 96 2 107 275 1 155 2.74e-19 85.5
Msa0338210 MtrunA17_Chr2g0325791 40.741 162 85 3 433 590 2 156 3.17e-18 87.0
Msa0338210 MtrunA17_Chr2g0325791 43.357 143 60 3 426 561 41 169 3.50e-16 80.9
Msa0338210 MtrunA17_Chr2g0325791 38.194 144 70 3 310 449 24 152 2.15e-11 66.2
Msa0338210 MtrunA17_Chr2g0325211 35.714 196 96 2 457 652 4 169 6.16e-14 71.2
Msa0338210 MtrunA17_Chr2g0325211 35.165 182 89 2 360 541 2 154 4.38e-11 62.8
Msa0338210 MtrunA17_Chr2g0325731 63.636 55 20 0 449 503 4 58 3.61e-12 62.4
Msa0338210 MtrunA17_Chr2g0325731 68.627 51 16 0 523 573 8 58 2.38e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338210 AT5G27220.4 26.822 645 410 18 187 806 195 802 3.34e-16 84.0
Msa0338210 AT5G27220.2 26.822 645 410 18 187 806 195 802 3.34e-16 84.0
Msa0338210 AT5G27220.1 26.822 645 410 18 187 806 195 802 4.89e-16 83.6
Msa0338210 AT5G27220.3 26.822 645 410 18 187 806 195 802 4.89e-16 83.6

Find 163 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACTCCTTGTGTCAATTTA+TGG 0.080642 2_4:+58416075 Msa0338210:CDS
CTCCTCAATATCTCGCTTTA+TGG 0.092724 2_4:-58413675 None:intergenic
TGAAAATTCGGTGGCAGTTC+TGG 0.150498 2_4:+58416047 Msa0338210:CDS
CTTTAGCAACTTCTTCCTTT+TGG 0.223344 2_4:-58414092 None:intergenic
TGCATCAAGCTCTCTCTCTT+TGG 0.269901 2_4:-58414029 None:intergenic
TCATTTGCACATTGAGCATT+AGG 0.277257 2_4:-58416981 None:intergenic
AGATCCATCAAAAGTTGTTT+TGG 0.291459 2_4:+58415843 Msa0338210:CDS
TGAATTTGCTTTGGAACTTA+AGG 0.312804 2_4:+58414006 Msa0338210:CDS
GTTTACCCGGATTTCGAGTT+TGG 0.322265 2_4:+58413388 Msa0338210:CDS
AGAGCTTGAAAATAAGAAAA+AGG 0.324967 2_4:+58413706 Msa0338210:CDS
TTTGATTTCTTAACAGAAAC+AGG 0.328488 2_4:-58413470 None:intergenic
TGTAGAGCTGTTTGGAACTC+TGG 0.329670 2_4:+58416167 Msa0338210:CDS
CAATCAACTGCTACCATTGA+TGG 0.335095 2_4:+58415757 Msa0338210:CDS
GAAGGAGGTGGAGTTTAAAA+AGG 0.337333 2_4:+58414444 Msa0338210:CDS
TGAAGATGATATTCCTTTGT+TGG 0.338336 2_4:+58413523 Msa0338210:CDS
GTTCCTCTTCCTTCCTTTCA+CGG 0.342021 2_4:-58413958 None:intergenic
TTACTATCGACTCCTTCTTT+TGG 0.343808 2_4:-58414564 None:intergenic
GCTATCAAACTGCTTCTCTT+TGG 0.353127 2_4:-58414785 None:intergenic
CCTTGTGTCAATTTATGGAT+TGG 0.361905 2_4:+58416080 Msa0338210:CDS
TAAAATAACCTTGATTTCAA+TGG 0.363499 2_4:-58416865 None:intergenic
TTGATGTGAGCTCCTCCATT+TGG 0.367926 2_4:-58414723 None:intergenic
GAATGAGAAGATTCTCAAAC+AGG 0.370642 2_4:+58417063 Msa0338210:three_prime_UTR
CTTTGTTGGATTTAATTAGA+AGG 0.375015 2_4:+58413537 Msa0338210:CDS
AAGTGAAAATACTGAAAATT+CGG 0.388056 2_4:+58416035 Msa0338210:CDS
GAAAATTCGGTGGCAGTTCT+GGG 0.395040 2_4:+58416048 Msa0338210:CDS
ATCGTTGGTTTGATTCTTGT+TGG 0.397145 2_4:-58416818 None:intergenic
GAATGTTCGTGCTCAAATAA+AGG 0.401392 2_4:+58414132 Msa0338210:CDS
AGAATTTCAGTTTCGTCACT+TGG 0.405145 2_4:-58415799 None:intergenic
TTTGACGAGGAAATTAAATC+AGG 0.407950 2_4:+58415192 Msa0338210:CDS
TAAACTCCACCTCCTTCATT+AGG 0.415653 2_4:-58414438 None:intergenic
TTCAAAAGCCGGCTCGAGTC+AGG 0.421084 2_4:+58414598 Msa0338210:CDS
GGAAGGGCAGCTCTATTTGA+TGG 0.424280 2_4:+58413814 Msa0338210:CDS
AATATGTGGACTGTGAAATC+AGG 0.425338 2_4:+58416953 Msa0338210:three_prime_UTR
TTGATTCATGCTCCTTCGCT+CGG 0.428334 2_4:-58414849 None:intergenic
TTGATTTAAACTCCTTCTCC+TGG 0.429349 2_4:-58414396 None:intergenic
TATTTGATGGATGACTTGAT+TGG 0.430087 2_4:+58413827 Msa0338210:CDS
AAGATAGCTGTAGAGCTGTT+TGG 0.433585 2_4:+58416159 Msa0338210:CDS
GTAGAGCTGTTTGGAACTCT+GGG 0.438058 2_4:+58416168 Msa0338210:CDS
AAAGAAGCAACTTGAAGGTC+AGG 0.440427 2_4:+58414168 Msa0338210:CDS
TGCGGTAATGGTGATGATGA+TGG 0.443280 2_4:+58413302 Msa0338210:CDS
TAGATTCCAGCTCCTTCACT+TGG 0.444751 2_4:-58414681 None:intergenic
AAAATGCAAAATACAAGAAA+AGG 0.451647 2_4:-58417117 None:intergenic
CGAAGCTCATGAGAAATTGC+AGG 0.452129 2_4:+58413754 Msa0338210:CDS
TTGATTCAAACTCCTTTACT+TGG 0.452256 2_4:-58414522 None:intergenic
CTTACACGAGGTTTAATATG+TGG 0.453106 2_4:-58415970 None:intergenic
GATTTATCAGTTGATTTGCT+TGG 0.454214 2_4:-58413560 None:intergenic
TTGAATTTAAAGATCGAGTG+AGG 0.457501 2_4:+58415046 Msa0338210:CDS
TCAATAAAGAAGCAACTTGA+AGG 0.464485 2_4:+58414163 Msa0338210:CDS
TCACCATTACCGCAAAACTT+TGG 0.464980 2_4:-58413293 None:intergenic
TCAAGAGAGAAGCAACTTGA+AGG 0.467554 2_4:+58414247 Msa0338210:CDS
CGTTGTTACAGCATCAAGCT+TGG 0.468875 2_4:+58412963 Msa0338210:five_prime_UTR
TCTTCTCTCCTGACTCGAGC+CGG 0.469898 2_4:-58414606 None:intergenic
AACGTGCAGGTGCATGAAGA+TGG 0.473081 2_4:-58416919 None:intergenic
CCTTTACTTGGCATTCAAGT+TGG 0.476149 2_4:-58414510 None:intergenic
AATCGTCTCCATTGAAATCA+AGG 0.477245 2_4:+58416857 Msa0338210:exon
TTGATGTGAGCTCCTCCACT+TGG 0.478027 2_4:-58414933 None:intergenic
GCTAAAGCTAACATCAGAAA+AGG 0.480977 2_4:+58414108 Msa0338210:CDS
TCAATGCAGAATGAATTTGA+TGG 0.481556 2_4:+58415114 Msa0338210:CDS
GCAATTGAAAGGACAAGTTA+AGG 0.483479 2_4:+58414963 Msa0338210:CDS
GAAGGAGTTTAAATCAAAAG+AGG 0.484213 2_4:+58414402 Msa0338210:CDS
GTCCATAAAGCGAGATATTG+AGG 0.485999 2_4:+58413673 Msa0338210:CDS
CTTTGTTACCGAGGTTTACC+CGG 0.489728 2_4:+58413375 Msa0338210:CDS
CATTATTCAAAAGTATAAGA+TGG 0.494090 2_4:+58415882 Msa0338210:CDS
GCAATTGAAAGGACAGGTTA+AGG 0.494228 2_4:+58414753 Msa0338210:CDS
TGATGTTTCTGTTAAGAAGA+GGG 0.496115 2_4:+58413418 Msa0338210:CDS
AGTCATTGATAATATTGATA+AGG 0.496791 2_4:+58413934 Msa0338210:CDS
GCAATGAACAAATTAATTAG+TGG 0.497881 2_4:+58414049 Msa0338210:CDS
TGAAATTCAAGCGAAGGAGC+TGG 0.502421 2_4:+58414885 Msa0338210:CDS
TCTAAGATGCAGGAACTTGA+AGG 0.507887 2_4:+58414289 Msa0338210:CDS
GGAGTTTGAAGCGAAGCAGT+TGG 0.508930 2_4:+58415068 Msa0338210:CDS
CTGCAAACTTCTTCCATCAA+TGG 0.511498 2_4:-58415770 None:intergenic
AAGAGAAGCAATTTGATAGC+CGG 0.511794 2_4:+58414998 Msa0338210:CDS
CAATGTGCAAATGAATTAGC+AGG 0.514188 2_4:+58416990 Msa0338210:three_prime_UTR
GAAACATGAAAGTCGGTTGA+AGG 0.517920 2_4:+58414627 Msa0338210:CDS
AGAGAAGCAGTTTGATAGCC+GGG 0.518876 2_4:+58414789 Msa0338210:CDS
GGAATTTGAATGCCAAAAGA+AGG 0.520295 2_4:+58414552 Msa0338210:CDS
GAAGCAATTCGAAAGCCAAA+TGG 0.522174 2_4:+58414708 Msa0338210:CDS
AGAATTTGAAATTCAAGCGA+AGG 0.522189 2_4:+58414879 Msa0338210:CDS
TCAAAAGAGGAGAAGTTTGA+AGG 0.522468 2_4:+58414415 Msa0338210:CDS
GTTGGTTGTAAATTAATATG+TGG 0.525022 2_4:+58416939 Msa0338210:three_prime_UTR
AGAGAAGCAATTTGATAGCC+GGG 0.528136 2_4:+58414999 Msa0338210:CDS
TCAAAAGAAAGAGAGTTTGA+AGG 0.528477 2_4:+58414658 Msa0338210:CDS
GAATTTCAGTTTCGTCACTT+GGG 0.529088 2_4:-58415798 None:intergenic
TTGATTCAAACGCTTTCACC+CGG 0.529873 2_4:-58415017 None:intergenic
TTGACTCTAACACATTCGAT+TGG 0.531740 2_4:-58414480 None:intergenic
AGAAATGCAACTTAAAGTCC+AGG 0.531894 2_4:+58414378 Msa0338210:CDS
CAATGAACAAATTAATTAGT+GGG 0.531973 2_4:+58414050 Msa0338210:CDS
ATTGTTAGAAAGCTCATTTG+AGG 0.533517 2_4:+58413610 Msa0338210:CDS
GGAGTTTGAATCTAAGATGC+AGG 0.536802 2_4:+58414279 Msa0338210:CDS
GAAGCAATGTGATGAACGGC+TGG 0.539147 2_4:+58414213 Msa0338210:CDS
AGAGAAGCAAGTCGAAGGAC+GGG 0.541425 2_4:+58414336 Msa0338210:CDS
TTAATTTACAACCAACGTGC+AGG 0.543527 2_4:-58416932 None:intergenic
CGAATTTGAAGGCCGAGCGA+AGG 0.543642 2_4:+58414837 Msa0338210:CDS
CCAACTTGAATGCCAAGTAA+AGG 0.544409 2_4:+58414510 Msa0338210:CDS
AAGAGAAGCAGTTTGATAGC+CGG 0.545775 2_4:+58414788 Msa0338210:CDS
TCAAAACTGTGGCAATTGAA+AGG 0.545936 2_4:+58414952 Msa0338210:CDS
ACTTCATCTTCATCGAAAGA+AGG 0.546038 2_4:-58416105 None:intergenic
GAAGTTTGAAGGCCTAATGA+AGG 0.546338 2_4:+58414426 Msa0338210:CDS
GCAAGTCGAAGGACGGGCGA+TGG 0.547167 2_4:+58414342 Msa0338210:CDS
GCGGTAATGGTGATGATGAT+GGG 0.548173 2_4:+58413303 Msa0338210:CDS
ATTATTCAAAAGTATAAGAT+GGG 0.548310 2_4:+58415883 Msa0338210:CDS
TCAAAAGAGGACGAATTTGA+AGG 0.549498 2_4:+58414826 Msa0338210:CDS
CTCGAAATCCGGGTAAACCT+CGG 0.550108 2_4:-58413383 None:intergenic
TGAATTTGATGGAGAACTGA+AGG 0.552236 2_4:+58415125 Msa0338210:CDS
GCAAAATACAAGAAAAGGAA+AGG 0.555119 2_4:-58417112 None:intergenic
GTGATGTTTCTGTTAAGAAG+AGG 0.556042 2_4:+58413417 Msa0338210:CDS
TCAAATCTGTGGCAATTGAA+AGG 0.559650 2_4:+58414742 Msa0338210:CDS
TTGCACATTGAGCATTAGGA+TGG 0.560311 2_4:-58416977 None:intergenic
TGATAAGGACCGTGAAAGGA+AGG 0.560715 2_4:+58413949 Msa0338210:CDS
TTAGAAAGCTCATTTGAGGA+AGG 0.561900 2_4:+58413614 Msa0338210:CDS
ACGTGCAGGTGCATGAAGAT+GGG 0.565339 2_4:-58416918 None:intergenic
GATAGAACTTAATCAAGTCA+AGG 0.567093 2_4:+58413880 Msa0338210:CDS
GAAAGCATTTGAATCAAAAG+AGG 0.571886 2_4:+58414813 Msa0338210:CDS
GAAAGCGTTTGAATCAAAAG+AGG 0.576259 2_4:+58415023 Msa0338210:CDS
TCTTGGATGCAAGAGATCGT+TGG 0.579943 2_4:-58416833 None:intergenic
AAGAGAAGCAAGTCGAAGGA+CGG 0.580246 2_4:+58414335 Msa0338210:CDS
TGAAGGCCAAGTGAAGGAGC+TGG 0.581942 2_4:+58414675 Msa0338210:CDS
ATGAGCTTTCTAACAATGCA+AGG 0.582002 2_4:-58413604 None:intergenic
GGAGTTTGAATCAAAGCAGA+AGG 0.582316 2_4:+58414531 Msa0338210:CDS
TTGATTCAAATGCTTTCACC+CGG 0.583537 2_4:-58414807 None:intergenic
GCAACTTGAAGGACACATGA+AGG 0.585195 2_4:+58414258 Msa0338210:CDS
GGACCGTGAAAGGAAGGAAG+AGG 0.585661 2_4:+58413955 Msa0338210:CDS
TGAAGGCCTAATGAAGGAGG+TGG 0.589996 2_4:+58414432 Msa0338210:CDS
TATGCATATATATTTCTCGA+AGG 0.591977 2_4:+58417142 Msa0338210:three_prime_UTR
AGAGTTTGAAGGCCAAGTGA+AGG 0.594285 2_4:+58414669 Msa0338210:CDS
CAAGCTAGAGCTCAAGACAA+AGG 0.596765 2_4:+58413856 Msa0338210:CDS
ATAGAACTTAATCAAGTCAA+GGG 0.597599 2_4:+58413881 Msa0338210:CDS
TGAATTTAAAGATCGAGTGA+GGG 0.599120 2_4:+58415047 Msa0338210:CDS
TCAAAAGAGAAGCAAGTCGA+AGG 0.600747 2_4:+58414331 Msa0338210:CDS
TGCGTGGATCAATAAAGAGA+GGG 0.601127 2_4:-58417018 None:intergenic
ATGATGTTCCTTTGTTACCG+AGG 0.602142 2_4:+58413366 Msa0338210:CDS
CTTCTAATTAAATCCAACAA+AGG 0.602945 2_4:-58413536 None:intergenic
CGGGTAAACCTCGGTAACAA+AGG 0.604343 2_4:-58413374 None:intergenic
GCAACTTAAAGTCCAGGAGA+AGG 0.605338 2_4:+58414384 Msa0338210:CDS
GCAACTTGAAGGTCAGGTCA+AGG 0.608454 2_4:+58414174 Msa0338210:CDS
TTTCTTAACAGAAACAGGCA+AGG 0.611151 2_4:-58413465 None:intergenic
GAGAGAAGAAACATGAAAGT+CGG 0.614493 2_4:+58414620 Msa0338210:CDS
AAGAGAAGCAATGTGATGAA+CGG 0.614667 2_4:+58414209 Msa0338210:CDS
TTGCGTGGATCAATAAAGAG+AGG 0.615054 2_4:-58417019 None:intergenic
TGAGGAAGGCAATAAGAAGA+CGG 0.615201 2_4:+58413628 Msa0338210:CDS
TGAAAATACTGAAAATTCGG+TGG 0.615980 2_4:+58416038 Msa0338210:CDS
GCAGTTGGAAGTGCAACTGA+AGG 0.616833 2_4:+58415083 Msa0338210:CDS
ATATTGATAAGGACCGTGAA+AGG 0.619247 2_4:+58413945 Msa0338210:CDS
TGTTTCTGTTAAGAAATCAA+AGG 0.624698 2_4:+58413472 Msa0338210:CDS
ATCTTCATGCACCTGCACGT+TGG 0.624742 2_4:+58416921 Msa0338210:three_prime_UTR
TCTGTGGCAATTGAAAGGAC+AGG 0.625484 2_4:+58414747 Msa0338210:CDS
GAAATTCTAGATAATCTGCA+AGG 0.625701 2_4:+58415814 Msa0338210:CDS
AAGGAAGGAAGAGGAACTCA+AGG 0.628919 2_4:+58413964 Msa0338210:CDS
GAAGCTCATGAGAAATTGCA+GGG 0.629107 2_4:+58413755 Msa0338210:CDS
GAAGGAGCTTGAGTTAAGAG+AGG 0.633737 2_4:+58415143 Msa0338210:CDS
TGCAAGGCCTTTCTTCAGCG+AGG 0.638330 2_4:-58413588 None:intergenic
GCAATTCGAAAGCCAAATGG+AGG 0.643602 2_4:+58414711 Msa0338210:CDS
TAATGGTGATGATGATGGGA+GGG 0.651761 2_4:+58413307 Msa0338210:CDS
TACAAGAAAAGGAAAGGACA+CGG 0.654271 2_4:-58417106 None:intergenic
CCAATCCATAAATTGACACA+AGG 0.656897 2_4:-58416080 None:intergenic
ATTGCTTCTTCTCTTACACG+AGG 0.664142 2_4:-58415982 None:intergenic
AATGAGAAGATTCTCAAACA+GGG 0.664747 2_4:+58417064 Msa0338210:three_prime_UTR
AGGAGCTCACATCAAATCTG+TGG 0.671175 2_4:+58414731 Msa0338210:CDS
GTTTGAAGGCCTAATGAAGG+AGG 0.671285 2_4:+58414429 Msa0338210:CDS
GTAATGGTGATGATGATGGG+AGG 0.675360 2_4:+58413306 Msa0338210:CDS
ATTACTAAAATCATTTGACG+AGG 0.688803 2_4:+58415179 Msa0338210:CDS
GCAATGTGATGAACGGCTGG+AGG 0.703208 2_4:+58414216 Msa0338210:CDS
AGGAGCTCACATCAAAACTG+TGG 0.719251 2_4:+58414941 Msa0338210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTGAAGAATGTATTA+AGG + chr2_4:58413778-58413797 Msa0338210:CDS 15.0%
!! ATTAAAAGATAAAGTTGAAA+TGG - chr2_4:58415254-58415273 None:intergenic 15.0%
!!! ACTTTATCTTTTAATATCTT+AGG + chr2_4:58415259-58415278 Msa0338210:intron 15.0%
!! ACACATATATTTAAATGTTT+GGG - chr2_4:58415609-58415628 None:intergenic 15.0%
!!! ATCTTATACTTTTGAATAAT+GGG - chr2_4:58415883-58415902 None:intergenic 15.0%
!!! ATTATTCAAAAGTATAAGAT+GGG + chr2_4:58415883-58415902 Msa0338210:CDS 15.0%
!!! GAAGTTTTTAGAATATCTTT+TGG - chr2_4:58412905-58412924 None:intergenic 20.0%
!! TGTTCAATTGAAAAATTTCT+AGG - chr2_4:58413195-58413214 None:intergenic 20.0%
!!! TTTTTTTGTTTTAATTTCCC+AGG + chr2_4:58413226-58413245 Msa0338210:intron 20.0%
!!! AGTCATTGATAATATTGATA+AGG + chr2_4:58413934-58413953 Msa0338210:CDS 20.0%
!! CAATGAACAAATTAATTAGT+GGG + chr2_4:58414050-58414069 Msa0338210:CDS 20.0%
!! CACACATATATTTAAATGTT+TGG - chr2_4:58415610-58415629 None:intergenic 20.0%
!!! TACTGATGTTTCTATAAATA+TGG + chr2_4:58415691-58415710 Msa0338210:intron 20.0%
!!! ACTGATGTTTCTATAAATAT+GGG + chr2_4:58415692-58415711 Msa0338210:intron 20.0%
!!! CATCTTATACTTTTGAATAA+TGG - chr2_4:58415884-58415903 None:intergenic 20.0%
!!! CATTATTCAAAAGTATAAGA+TGG + chr2_4:58415882-58415901 Msa0338210:CDS 20.0%
!! AAGTGAAAATACTGAAAATT+CGG + chr2_4:58416035-58416054 Msa0338210:CDS 20.0%
!! AAGAAAATAAGTATAATAGC+TGG - chr2_4:58416247-58416266 None:intergenic 20.0%
!! ATGATTAAGATATTATCTGT+TGG + chr2_4:58416356-58416375 Msa0338210:intron 20.0%
!!! TCTATTTCTTAAAATGTTTG+AGG + chr2_4:58416600-58416619 Msa0338210:intron 20.0%
!! TAAAATAACCTTGATTTCAA+TGG - chr2_4:58416868-58416887 None:intergenic 20.0%
!! AAAATGCAAAATACAAGAAA+AGG - chr2_4:58417120-58417139 None:intergenic 20.0%
! ACAATAAACAAGTACAATGA+CGG - chr2_4:58413120-58413139 None:intergenic 25.0%
!! TTATTGTTTCATTTTGCTTC+TGG + chr2_4:58413133-58413152 Msa0338210:intron 25.0%
!! TACTTGAATGATGAATTTTG+AGG + chr2_4:58413259-58413278 Msa0338210:exon 25.0%
!! TTTGATTTCTTAACAGAAAC+AGG - chr2_4:58413473-58413492 None:intergenic 25.0%
!! TGTTTCTGTTAAGAAATCAA+AGG + chr2_4:58413472-58413491 Msa0338210:CDS 25.0%
! CTTCTAATTAAATCCAACAA+AGG - chr2_4:58413539-58413558 None:intergenic 25.0%
! CTTTGTTGGATTTAATTAGA+AGG + chr2_4:58413537-58413556 Msa0338210:CDS 25.0%
! AGAGCTTGAAAATAAGAAAA+AGG + chr2_4:58413706-58413725 Msa0338210:CDS 25.0%
!!! AAGGAAATTAGTTGTTTTAG+AGG + chr2_4:58413725-58413744 Msa0338210:CDS 25.0%
!! TATTAAGGATTTTGTAGTGA+AGG + chr2_4:58413793-58413812 Msa0338210:CDS 25.0%
! ATAGAACTTAATCAAGTCAA+GGG + chr2_4:58413881-58413900 Msa0338210:CDS 25.0%
!!! AGCAAATTCAGCAATTTTTT+GGG - chr2_4:58413996-58414015 None:intergenic 25.0%
!!! AAGCAAATTCAGCAATTTTT+TGG - chr2_4:58413997-58414016 None:intergenic 25.0%
!! AAAAATTGCTGAATTTGCTT+TGG + chr2_4:58413997-58414016 Msa0338210:CDS 25.0%
! GCAATGAACAAATTAATTAG+TGG + chr2_4:58414049-58414068 Msa0338210:CDS 25.0%
! GACAAAAATATTTCAAAAGC+CGG + chr2_4:58414587-58414606 Msa0338210:CDS 25.0%
! ATTACTAAAATCATTTGACG+AGG + chr2_4:58415179-58415198 Msa0338210:CDS 25.0%
!!! GAGTTTCAAGGTTTTTTTTT+TGG + chr2_4:58415403-58415422 Msa0338210:intron 25.0%
! ATAGAAACATCAGTAAATCA+AGG - chr2_4:58415686-58415705 None:intergenic 25.0%
!! ATATCCAAAACAACTTTTGA+TGG - chr2_4:58415850-58415869 None:intergenic 25.0%
!! TTCAGTATTTTCACTTATGT+TGG - chr2_4:58416031-58416050 None:intergenic 25.0%
!!! GATGAAGTTTTAAAGCTTTT+CGG + chr2_4:58416120-58416139 Msa0338210:CDS 25.0%
!!! ATGAAGTTTTAAAGCTTTTC+GGG + chr2_4:58416121-58416140 Msa0338210:CDS 25.0%
! TTAAGATATTATCTGTTGGT+AGG + chr2_4:58416360-58416379 Msa0338210:intron 25.0%
!!! GTTTTGTTGAAGTGAAATTT+TGG + chr2_4:58416712-58416731 Msa0338210:intron 25.0%
! GTTGGTTGTAAATTAATATG+TGG + chr2_4:58416939-58416958 Msa0338210:three_prime_UTR 25.0%
! TATGCATATATATTTCTCGA+AGG + chr2_4:58417142-58417161 Msa0338210:three_prime_UTR 25.0%
ACAACGCTAAAAGAGAAAAA+CGG - chr2_4:58412949-58412968 None:intergenic 30.0%
CATGAAGTAATGCTACATTA+CGG - chr2_4:58413161-58413180 None:intergenic 30.0%
TCAAGTAATTTACACTTCCT+GGG - chr2_4:58413246-58413265 None:intergenic 30.0%
TTCAAGTAATTTACACTTCC+TGG - chr2_4:58413247-58413266 None:intergenic 30.0%
! TTTGATAAGCCAAAGTTTTG+CGG + chr2_4:58413284-58413303 Msa0338210:CDS 30.0%
! ATTTTACCAAACTCGAAATC+CGG - chr2_4:58413397-58413416 None:intergenic 30.0%
! TGATGTTTCTGTTAAGAAGA+GGG + chr2_4:58413418-58413437 Msa0338210:CDS 30.0%
TGAAGATGATATTCCTTTGT+TGG + chr2_4:58413523-58413542 Msa0338210:CDS 30.0%
!! GATTTATCAGTTGATTTGCT+TGG - chr2_4:58413563-58413582 None:intergenic 30.0%
ATTGTTAGAAAGCTCATTTG+AGG + chr2_4:58413610-58413629 Msa0338210:CDS 30.0%
! TATTTGATGGATGACTTGAT+TGG + chr2_4:58413827-58413846 Msa0338210:CDS 30.0%
GATAGAACTTAATCAAGTCA+AGG + chr2_4:58413880-58413899 Msa0338210:CDS 30.0%
!! TGAATTTGCTTTGGAACTTA+AGG + chr2_4:58414006-58414025 Msa0338210:CDS 30.0%
CAGAAAAATTAGAGTCCAAA+AGG + chr2_4:58414077-58414096 Msa0338210:CDS 30.0%
TCAATAAAGAAGCAACTTGA+AGG + chr2_4:58414163-58414182 Msa0338210:CDS 30.0%
GAAGGAGTTTAAATCAAAAG+AGG + chr2_4:58414402-58414421 Msa0338210:CDS 30.0%
TTGATTCAAACTCCTTTACT+TGG - chr2_4:58414525-58414544 None:intergenic 30.0%
TCAAAAGAAAGAGAGTTTGA+AGG + chr2_4:58414658-58414677 Msa0338210:CDS 30.0%
!! GAAAGCATTTGAATCAAAAG+AGG + chr2_4:58414813-58414832 Msa0338210:CDS 30.0%
! AGAATTTGAAATTCAAGCGA+AGG + chr2_4:58414879-58414898 Msa0338210:CDS 30.0%
TTGAATTTAAAGATCGAGTG+AGG + chr2_4:58415046-58415065 Msa0338210:CDS 30.0%
TGAATTTAAAGATCGAGTGA+GGG + chr2_4:58415047-58415066 Msa0338210:CDS 30.0%
TCAATGCAGAATGAATTTGA+TGG + chr2_4:58415114-58415133 Msa0338210:CDS 30.0%
TTTGACGAGGAAATTAAATC+AGG + chr2_4:58415192-58415211 Msa0338210:CDS 30.0%
!!! AGGTTTTATTCTTGTCGAAT+CGG + chr2_4:58415279-58415298 Msa0338210:intron 30.0%
TATGATATGACTGAGTTTCA+AGG + chr2_4:58415391-58415410 Msa0338210:intron 30.0%
!!! TCAAGGTTTTTTTTTTGGAG+AGG + chr2_4:58415408-58415427 Msa0338210:intron 30.0%
! TCAGTTGATACTAACAGTAA+CGG - chr2_4:58415577-58415596 None:intergenic 30.0%
GAAATTCTAGATAATCTGCA+AGG + chr2_4:58415814-58415833 Msa0338210:CDS 30.0%
! AGATCCATCAAAAGTTGTTT+TGG + chr2_4:58415843-58415862 Msa0338210:CDS 30.0%
TGAAAATACTGAAAATTCGG+TGG + chr2_4:58416038-58416057 Msa0338210:CDS 30.0%
ATATATCATGTAGAGTCTCT+AGG - chr2_4:58416323-58416342 None:intergenic 30.0%
AAGCTGAACTGATATGTAAT+TGG + chr2_4:58416383-58416402 Msa0338210:intron 30.0%
!!! TCTGCTTAGTTTTGGTTAAT+TGG + chr2_4:58416663-58416682 Msa0338210:intron 30.0%
AATGAGAAGATTCTCAAACA+GGG + chr2_4:58417064-58417083 Msa0338210:three_prime_UTR 30.0%
GCAAAATACAAGAAAAGGAA+AGG - chr2_4:58417115-58417134 None:intergenic 30.0%
AGAGAAAAACGGAAGATGAA+AGG - chr2_4:58412938-58412957 None:intergenic 35.0%
TGACGGTGTAAATTAGTTAC+AGG - chr2_4:58413103-58413122 None:intergenic 35.0%
! TTTTACCAAACTCGAAATCC+GGG - chr2_4:58413396-58413415 None:intergenic 35.0%
! GTGATGTTTCTGTTAAGAAG+AGG + chr2_4:58413417-58413436 Msa0338210:CDS 35.0%
TTTCTTAACAGAAACAGGCA+AGG - chr2_4:58413468-58413487 None:intergenic 35.0%
ATGAGCTTTCTAACAATGCA+AGG - chr2_4:58413607-58413626 None:intergenic 35.0%
TTAGAAAGCTCATTTGAGGA+AGG + chr2_4:58413614-58413633 Msa0338210:CDS 35.0%
! AAGGATTTTGTAGTGAAGGA+AGG + chr2_4:58413797-58413816 Msa0338210:CDS 35.0%
! AGGATTTTGTAGTGAAGGAA+GGG + chr2_4:58413798-58413817 Msa0338210:CDS 35.0%
ATATTGATAAGGACCGTGAA+AGG + chr2_4:58413945-58413964 Msa0338210:CDS 35.0%
! CTTTAGCAACTTCTTCCTTT+TGG - chr2_4:58414095-58414114 None:intergenic 35.0%
GCTAAAGCTAACATCAGAAA+AGG + chr2_4:58414108-58414127 Msa0338210:CDS 35.0%
GAATGTTCGTGCTCAAATAA+AGG + chr2_4:58414132-58414151 Msa0338210:CDS 35.0%
AAGAGAAGCAATGTGATGAA+CGG + chr2_4:58414209-58414228 Msa0338210:CDS 35.0%
AGAAATGCAACTTAAAGTCC+AGG + chr2_4:58414378-58414397 Msa0338210:CDS 35.0%
TTGATTTAAACTCCTTCTCC+TGG - chr2_4:58414399-58414418 None:intergenic 35.0%
TCAAAAGAGGAGAAGTTTGA+AGG + chr2_4:58414415-58414434 Msa0338210:CDS 35.0%
TTGACTCTAACACATTCGAT+TGG - chr2_4:58414483-58414502 None:intergenic 35.0%
GGAATTTGAATGCCAAAAGA+AGG + chr2_4:58414552-58414571 Msa0338210:CDS 35.0%
!!! TTACTATCGACTCCTTCTTT+TGG - chr2_4:58414567-58414586 None:intergenic 35.0%
GAGAGAAGAAACATGAAAGT+CGG + chr2_4:58414620-58414639 Msa0338210:CDS 35.0%
TCAAATCTGTGGCAATTGAA+AGG + chr2_4:58414742-58414761 Msa0338210:CDS 35.0%
TTGATTCAAATGCTTTCACC+CGG - chr2_4:58414810-58414829 None:intergenic 35.0%
TCAAAAGAGGACGAATTTGA+AGG + chr2_4:58414826-58414845 Msa0338210:CDS 35.0%
TCAAAACTGTGGCAATTGAA+AGG + chr2_4:58414952-58414971 Msa0338210:CDS 35.0%
GCAATTGAAAGGACAAGTTA+AGG + chr2_4:58414963-58414982 Msa0338210:CDS 35.0%
! AAGAGAAGCAATTTGATAGC+CGG + chr2_4:58414998-58415017 Msa0338210:CDS 35.0%
!! GAAAGCGTTTGAATCAAAAG+AGG + chr2_4:58415023-58415042 Msa0338210:CDS 35.0%
TGAATTTGATGGAGAACTGA+AGG + chr2_4:58415125-58415144 Msa0338210:CDS 35.0%
GGTAAATGATCTTCATCATC+TGG + chr2_4:58415213-58415232 Msa0338210:intron 35.0%
!!! AGGTTTTTTTTTTGGAGAGG+AGG + chr2_4:58415411-58415430 Msa0338210:intron 35.0%
TTCAATACCCTGACAAACTA+TGG + chr2_4:58415450-58415469 Msa0338210:intron 35.0%
AACAGCAATAGTTGCACTAA+AGG - chr2_4:58415490-58415509 None:intergenic 35.0%
GAATTTCAGTTTCGTCACTT+GGG - chr2_4:58415801-58415820 None:intergenic 35.0%
AGAATTTCAGTTTCGTCACT+TGG - chr2_4:58415802-58415821 None:intergenic 35.0%
CTTACACGAGGTTTAATATG+TGG - chr2_4:58415973-58415992 None:intergenic 35.0%
CTACTCCTTGTGTCAATTTA+TGG + chr2_4:58416075-58416094 Msa0338210:CDS 35.0%
CCAATCCATAAATTGACACA+AGG - chr2_4:58416083-58416102 None:intergenic 35.0%
CCTTGTGTCAATTTATGGAT+TGG + chr2_4:58416080-58416099 Msa0338210:CDS 35.0%
ACTTCATCTTCATCGAAAGA+AGG - chr2_4:58416108-58416127 None:intergenic 35.0%
TATGAAGCAAAGACTCTACA+CGG - chr2_4:58416554-58416573 None:intergenic 35.0%
TAACCAAAACTAAGCAGACT+GGG - chr2_4:58416661-58416680 None:intergenic 35.0%
TTAACCAAAACTAAGCAGAC+TGG - chr2_4:58416662-58416681 None:intergenic 35.0%
!! GTTAATTGGAACTGACTTGA+TGG + chr2_4:58416677-58416696 Msa0338210:intron 35.0%
GTTTCTGAACAAAATCTGCA+AGG - chr2_4:58416742-58416761 None:intergenic 35.0%
!! ATCGTTGGTTTGATTCTTGT+TGG - chr2_4:58416821-58416840 None:intergenic 35.0%
! TTCAATGGAGACGATTTTCT+TGG - chr2_4:58416853-58416872 None:intergenic 35.0%
AATCGTCTCCATTGAAATCA+AGG + chr2_4:58416857-58416876 Msa0338210:exon 35.0%
TTAATTTACAACCAACGTGC+AGG - chr2_4:58416935-58416954 None:intergenic 35.0%
! AATATGTGGACTGTGAAATC+AGG + chr2_4:58416953-58416972 Msa0338210:three_prime_UTR 35.0%
TCATTTGCACATTGAGCATT+AGG - chr2_4:58416984-58417003 None:intergenic 35.0%
CAATGTGCAAATGAATTAGC+AGG + chr2_4:58416990-58417009 Msa0338210:three_prime_UTR 35.0%
! ACAATAACAGACATTTTGCG+TGG - chr2_4:58417037-58417056 None:intergenic 35.0%
GAATGAGAAGATTCTCAAAC+AGG + chr2_4:58417063-58417082 Msa0338210:three_prime_UTR 35.0%
! CAGGGTTATCTCAATTTTGT+TGG + chr2_4:58417082-58417101 Msa0338210:three_prime_UTR 35.0%
TACAAGAAAAGGAAAGGACA+CGG - chr2_4:58417109-58417128 None:intergenic 35.0%
! AAGCCAAAGTTTTGCGGTAA+TGG + chr2_4:58413290-58413309 Msa0338210:CDS 40.0%
TCACCATTACCGCAAAACTT+TGG - chr2_4:58413296-58413315 None:intergenic 40.0%
!! TAATGGTGATGATGATGGGA+GGG + chr2_4:58413307-58413326 Msa0338210:CDS 40.0%
ATGATGTTCCTTTGTTACCG+AGG + chr2_4:58413366-58413385 Msa0338210:CDS 40.0%
! TCATTTTCCTCGCTGAAGAA+AGG + chr2_4:58413581-58413600 Msa0338210:CDS 40.0%
TGAGGAAGGCAATAAGAAGA+CGG + chr2_4:58413628-58413647 Msa0338210:CDS 40.0%
GTCCATAAAGCGAGATATTG+AGG + chr2_4:58413673-58413692 Msa0338210:CDS 40.0%
CTCCTCAATATCTCGCTTTA+TGG - chr2_4:58413678-58413697 None:intergenic 40.0%
GAAGCTCATGAGAAATTGCA+GGG + chr2_4:58413755-58413774 Msa0338210:CDS 40.0%
AAAGAAGCAACTTGAAGGTC+AGG + chr2_4:58414168-58414187 Msa0338210:CDS 40.0%
TCAAGAGAGAAGCAACTTGA+AGG + chr2_4:58414247-58414266 Msa0338210:CDS 40.0%
! GGAGTTTGAATCTAAGATGC+AGG + chr2_4:58414279-58414298 Msa0338210:CDS 40.0%
! TCTAAGATGCAGGAACTTGA+AGG + chr2_4:58414289-58414308 Msa0338210:CDS 40.0%
TCAAAAGAGAAGCAAGTCGA+AGG + chr2_4:58414331-58414350 Msa0338210:CDS 40.0%
GAAGTTTGAAGGCCTAATGA+AGG + chr2_4:58414426-58414445 Msa0338210:CDS 40.0%
TAAACTCCACCTCCTTCATT+AGG - chr2_4:58414441-58414460 None:intergenic 40.0%
GAAGGAGGTGGAGTTTAAAA+AGG + chr2_4:58414444-58414463 Msa0338210:CDS 40.0%
CCTTTACTTGGCATTCAAGT+TGG - chr2_4:58414513-58414532 None:intergenic 40.0%
CCAACTTGAATGCCAAGTAA+AGG + chr2_4:58414510-58414529 Msa0338210:CDS 40.0%
GGAGTTTGAATCAAAGCAGA+AGG + chr2_4:58414531-58414550 Msa0338210:CDS 40.0%
GAAACATGAAAGTCGGTTGA+AGG + chr2_4:58414627-58414646 Msa0338210:CDS 40.0%
GAAGCAATTCGAAAGCCAAA+TGG + chr2_4:58414708-58414727 Msa0338210:CDS 40.0%
GCAATTGAAAGGACAGGTTA+AGG + chr2_4:58414753-58414772 Msa0338210:CDS 40.0%
GCTATCAAACTGCTTCTCTT+TGG - chr2_4:58414788-58414807 None:intergenic 40.0%
! AAGAGAAGCAGTTTGATAGC+CGG + chr2_4:58414788-58414807 Msa0338210:CDS 40.0%
!! GAAGCATTTTGAAAGCCAAG+TGG + chr2_4:58414918-58414937 Msa0338210:CDS 40.0%
! AGAGAAGCAATTTGATAGCC+GGG + chr2_4:58414999-58415018 Msa0338210:CDS 40.0%
TTGATTCAAACGCTTTCACC+CGG - chr2_4:58415020-58415039 None:intergenic 40.0%
AATACCCTGACAAACTATGG+AGG + chr2_4:58415453-58415472 Msa0338210:intron 40.0%
! AATGCCTCCATAGTTTGTCA+GGG - chr2_4:58415460-58415479 None:intergenic 40.0%
! AAATGCCTCCATAGTTTGTC+AGG - chr2_4:58415461-58415480 None:intergenic 40.0%
CTTTGTTCTTACGCAACTTC+TGG + chr2_4:58415520-58415539 Msa0338210:intron 40.0%
AACTTCTGGTGATTCAGTTG+CGG + chr2_4:58415534-58415553 Msa0338210:intron 40.0%
CAATCAACTGCTACCATTGA+TGG + chr2_4:58415757-58415776 Msa0338210:CDS 40.0%
CTGCAAACTTCTTCCATCAA+TGG - chr2_4:58415773-58415792 None:intergenic 40.0%
ATTGCTTCTTCTCTTACACG+AGG - chr2_4:58415985-58416004 None:intergenic 40.0%
AAGATAGCTGTAGAGCTGTT+TGG + chr2_4:58416159-58416178 Msa0338210:CDS 40.0%
! GGTTTTGCTGATAAAGCTTC+TGG + chr2_4:58416189-58416208 Msa0338210:CDS 40.0%
!! ATGAAGCACCGACATAGATT+TGG + chr2_4:58416473-58416492 Msa0338210:intron 40.0%
TTGCACATTGAGCATTAGGA+TGG - chr2_4:58416980-58416999 None:intergenic 40.0%
TGCGTGGATCAATAAAGAGA+GGG - chr2_4:58417021-58417040 None:intergenic 40.0%
TTGCGTGGATCAATAAAGAG+AGG - chr2_4:58417022-58417041 None:intergenic 40.0%
CGTTGTTACAGCATCAAGCT+TGG + chr2_4:58412963-58412982 Msa0338210:five_prime_UTR 45.0%
! TGCGGTAATGGTGATGATGA+TGG + chr2_4:58413302-58413321 Msa0338210:CDS 45.0%
! GCGGTAATGGTGATGATGAT+GGG + chr2_4:58413303-58413322 Msa0338210:CDS 45.0%
!! GTAATGGTGATGATGATGGG+AGG + chr2_4:58413306-58413325 Msa0338210:CDS 45.0%
CTTTGTTACCGAGGTTTACC+CGG + chr2_4:58413375-58413394 Msa0338210:CDS 45.0%
!! GTTTACCCGGATTTCGAGTT+TGG + chr2_4:58413388-58413407 Msa0338210:CDS 45.0%
CGAAGCTCATGAGAAATTGC+AGG + chr2_4:58413754-58413773 Msa0338210:CDS 45.0%
CAAGCTAGAGCTCAAGACAA+AGG + chr2_4:58413856-58413875 Msa0338210:CDS 45.0%
TGATAAGGACCGTGAAAGGA+AGG + chr2_4:58413949-58413968 Msa0338210:CDS 45.0%
GTTCCTCTTCCTTCCTTTCA+CGG - chr2_4:58413961-58413980 None:intergenic 45.0%
AAGGAAGGAAGAGGAACTCA+AGG + chr2_4:58413964-58413983 Msa0338210:CDS 45.0%
TGCATCAAGCTCTCTCTCTT+TGG - chr2_4:58414032-58414051 None:intergenic 45.0%
GCAACTTGAAGGACACATGA+AGG + chr2_4:58414258-58414277 Msa0338210:CDS 45.0%
AAGAGAAGCAAGTCGAAGGA+CGG + chr2_4:58414335-58414354 Msa0338210:CDS 45.0%
GCAACTTAAAGTCCAGGAGA+AGG + chr2_4:58414384-58414403 Msa0338210:CDS 45.0%
GTTTGAAGGCCTAATGAAGG+AGG + chr2_4:58414429-58414448 Msa0338210:CDS 45.0%
AGAGTTTGAAGGCCAAGTGA+AGG + chr2_4:58414669-58414688 Msa0338210:CDS 45.0%
TAGATTCCAGCTCCTTCACT+TGG - chr2_4:58414684-58414703 None:intergenic 45.0%
GCAATTCGAAAGCCAAATGG+AGG + chr2_4:58414711-58414730 Msa0338210:CDS 45.0%
TTGATGTGAGCTCCTCCATT+TGG - chr2_4:58414726-58414745 None:intergenic 45.0%
AGGAGCTCACATCAAATCTG+TGG + chr2_4:58414731-58414750 Msa0338210:CDS 45.0%
TCTGTGGCAATTGAAAGGAC+AGG + chr2_4:58414747-58414766 Msa0338210:CDS 45.0%
! AGAGAAGCAGTTTGATAGCC+GGG + chr2_4:58414789-58414808 Msa0338210:CDS 45.0%
TTGATTCATGCTCCTTCGCT+CGG - chr2_4:58414852-58414871 None:intergenic 45.0%
TGAAATTCAAGCGAAGGAGC+TGG + chr2_4:58414885-58414904 Msa0338210:CDS 45.0%
!! GCATTTTGAAAGCCAAGTGG+AGG + chr2_4:58414921-58414940 Msa0338210:CDS 45.0%
AGGAGCTCACATCAAAACTG+TGG + chr2_4:58414941-58414960 Msa0338210:CDS 45.0%
GAAGGAGCTTGAGTTAAGAG+AGG + chr2_4:58415143-58415162 Msa0338210:CDS 45.0%
GAGGAGGACTATGAGTTTCA+AGG + chr2_4:58415427-58415446 Msa0338210:intron 45.0%
! TGAAAATTCGGTGGCAGTTC+TGG + chr2_4:58416047-58416066 Msa0338210:CDS 45.0%
! GAAAATTCGGTGGCAGTTCT+GGG + chr2_4:58416048-58416067 Msa0338210:CDS 45.0%
! TGTAGAGCTGTTTGGAACTC+TGG + chr2_4:58416167-58416186 Msa0338210:CDS 45.0%
! GTAGAGCTGTTTGGAACTCT+GGG + chr2_4:58416168-58416187 Msa0338210:CDS 45.0%
GGCTTCTTACACTTGATGCT+TGG + chr2_4:58416404-58416423 Msa0338210:intron 45.0%
CACGGACACCAAATCTATGT+CGG - chr2_4:58416484-58416503 None:intergenic 45.0%
ACACTGACGCATGTCTGAAA+CGG - chr2_4:58416531-58416550 None:intergenic 45.0%
TCTTGGATGCAAGAGATCGT+TGG - chr2_4:58416836-58416855 None:intergenic 45.0%
CGGGTAAACCTCGGTAACAA+AGG - chr2_4:58413377-58413396 None:intergenic 50.0%
CTCGAAATCCGGGTAAACCT+CGG - chr2_4:58413386-58413405 None:intergenic 50.0%
GGAAGGGCAGCTCTATTTGA+TGG + chr2_4:58413814-58413833 Msa0338210:CDS 50.0%
GCAACTTGAAGGTCAGGTCA+AGG + chr2_4:58414174-58414193 Msa0338210:CDS 50.0%
GAAGCAATGTGATGAACGGC+TGG + chr2_4:58414213-58414232 Msa0338210:CDS 50.0%
AGAGAAGCAAGTCGAAGGAC+GGG + chr2_4:58414336-58414355 Msa0338210:CDS 50.0%
TGAAGGCCTAATGAAGGAGG+TGG + chr2_4:58414432-58414451 Msa0338210:CDS 50.0%
TTGATGTGAGCTCCTCCACT+TGG - chr2_4:58414936-58414955 None:intergenic 50.0%
! GGAGTTTGAAGCGAAGCAGT+TGG + chr2_4:58415068-58415087 Msa0338210:CDS 50.0%
! GCAGTTGGAAGTGCAACTGA+AGG + chr2_4:58415083-58415102 Msa0338210:CDS 50.0%
! TTGGTGTCCGTGTAGAGTCT+CGG + chr2_4:58416492-58416511 Msa0338210:intron 50.0%
! ACTGACGCATGTCTGAAACG+GGG - chr2_4:58416529-58416548 None:intergenic 50.0%
! CACTGACGCATGTCTGAAAC+GGG - chr2_4:58416530-58416549 None:intergenic 50.0%
! CAGCCCAGTCTGCTTAGTTT+TGG + chr2_4:58416655-58416674 Msa0338210:intron 50.0%
ACGTGCAGGTGCATGAAGAT+GGG - chr2_4:58416921-58416940 None:intergenic 50.0%
AACGTGCAGGTGCATGAAGA+TGG - chr2_4:58416922-58416941 None:intergenic 50.0%
ATCTTCATGCACCTGCACGT+TGG + chr2_4:58416921-58416940 Msa0338210:three_prime_UTR 50.0%
TGCAAGGCCTTTCTTCAGCG+AGG - chr2_4:58413591-58413610 None:intergenic 55.0%
GGACCGTGAAAGGAAGGAAG+AGG + chr2_4:58413955-58413974 Msa0338210:CDS 55.0%
GCAATGTGATGAACGGCTGG+AGG + chr2_4:58414216-58414235 Msa0338210:CDS 55.0%
!! TTCAAAAGCCGGCTCGAGTC+AGG + chr2_4:58414598-58414617 Msa0338210:CDS 55.0%
! TCTTCTCTCCTGACTCGAGC+CGG - chr2_4:58414609-58414628 None:intergenic 55.0%
TGAAGGCCAAGTGAAGGAGC+TGG + chr2_4:58414675-58414694 Msa0338210:CDS 55.0%
CGAATTTGAAGGCCGAGCGA+AGG + chr2_4:58414837-58414856 Msa0338210:CDS 55.0%
!!! GCTTTTCGGGTTTGCTGCTC+AGG + chr2_4:58416134-58416153 Msa0338210:CDS 55.0%
TGGTGTCCGTGTAGAGTCTC+GGG + chr2_4:58416493-58416512 Msa0338210:intron 55.0%
TCCGTGTAGAGTCTCGGGTA+AGG + chr2_4:58416498-58416517 Msa0338210:intron 55.0%
GCCTTACCCGAGACTCTACA+CGG - chr2_4:58416502-58416521 None:intergenic 55.0%
GCAAGTCGAAGGACGGGCGA+TGG + chr2_4:58414342-58414361 Msa0338210:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 58412895 58417173 58412895 ID=Msa0338210;Name=Msa0338210
chr2_4 mRNA 58412895 58417173 58412895 ID=Msa0338210-mRNA-1;Parent=Msa0338210;Name=Msa0338210-mRNA-1;_AED=0.03;_eAED=0.03;_QI=119|0.66|0.75|1|0.66|0.5|4|302|848
chr2_4 exon 58412895 58412995 58412895 ID=Msa0338210-mRNA-1:exon:17968;Parent=Msa0338210-mRNA-1
chr2_4 exon 58413248 58415213 58413248 ID=Msa0338210-mRNA-1:exon:17969;Parent=Msa0338210-mRNA-1
chr2_4 exon 58415737 58416210 58415737 ID=Msa0338210-mRNA-1:exon:17970;Parent=Msa0338210-mRNA-1
chr2_4 exon 58416747 58417173 58416747 ID=Msa0338210-mRNA-1:exon:17971;Parent=Msa0338210-mRNA-1
chr2_4 five_prime_UTR 58412895 58412995 58412895 ID=Msa0338210-mRNA-1:five_prime_utr;Parent=Msa0338210-mRNA-1
chr2_4 five_prime_UTR 58413248 58413265 58413248 ID=Msa0338210-mRNA-1:five_prime_utr;Parent=Msa0338210-mRNA-1
chr2_4 CDS 58413266 58415213 58413266 ID=Msa0338210-mRNA-1:cds;Parent=Msa0338210-mRNA-1
chr2_4 CDS 58415737 58416210 58415737 ID=Msa0338210-mRNA-1:cds;Parent=Msa0338210-mRNA-1
chr2_4 CDS 58416747 58416871 58416747 ID=Msa0338210-mRNA-1:cds;Parent=Msa0338210-mRNA-1
chr2_4 three_prime_UTR 58416872 58417173 58416872 ID=Msa0338210-mRNA-1:three_prime_utr;Parent=Msa0338210-mRNA-1
Gene Sequence

>Msa0338210

ATGATGAATTTTGAGGCTTTTGATAAGCCAAAGTTTTGCGGTAATGGTGATGATGATGGGAGGGATCATGTTTCTTGTGAACACTTTGAATCTGAAGATGATGATGTTCCTTTGTTACCGAGGTTTACCCGGATTTCGAGTTTGGTAAAATGTGATGTTTCTGTTAAGAAGAGGGATTTTGAGTTTGATGACAACAATTCCTTGCCTGTTTCTGTTAAGAAATCAAAGGTGTCACTGTATCATGATCACGACAAAGATGAAGATGATATTCCTTTGTTGGATTTAATTAGAAGGCCAAGCAAATCAACTGATAAATCATTTTCCTCGCTGAAGAAAGGCCTTGCATTGTTAGAAAGCTCATTTGAGGAAGGCAATAAGAAGACGGAATTAGAAGAGAATAGATTGCAGTCCATAAAGCGAGATATTGAGGAGTGTCGCAAAGAGCTTGAAAATAAGAAAAAGGAAATTAGTTGTTTTAGAGGAATTGACGAAGCTCATGAGAAATTGCAGGGAAAAATTGAAGAATGTATTAAGGATTTTGTAGTGAAGGAAGGGCAGCTCTATTTGATGGATGACTTGATTGGAGAGTGCAAGCTAGAGCTCAAGACAAAGGAGATAGAACTTAATCAAGTCAAGGGCAACATTTCAAAAGAAATAGAACTTCGTCAAGTCATTGATAATATTGATAAGGACCGTGAAAGGAAGGAAGAGGAACTCAAGGCTCTTTCCCAAAAAATTGCTGAATTTGCTTTGGAACTTAAGGCCAAAGAGAGAGAGCTTGATGCAATGAACAAATTAATTAGTGGGAAAGCAGAAAAATTAGAGTCCAAAAGGAAGAAGTTGCTAAAGCTAACATCAGAAAAGGAGAATGTTCGTGCTCAAATAAAGGAATTTGAGTCAATAAAGAAGCAACTTGAAGGTCAGGTCAAGGAACTTGAGTCAAAAGAGAAGCAATGTGATGAACGGCTGGAGGTGTTGATATCAAGAGAGAAGCAACTTGAAGGACACATGAAGGAGTTTGAATCTAAGATGCAGGAACTTGAAGGTCGTACGATGCAGCTTGAATCAAAAGAGAAGCAAGTCGAAGGACGGGCGATGGAGCTAAAGTTAAAAGAAATGCAACTTAAAGTCCAGGAGAAGGAGTTTAAATCAAAAGAGGAGAAGTTTGAAGGCCTAATGAAGGAGGTGGAGTTTAAAAAGGAGCACTTTGAAAACCAATCGAATGTGTTAGAGTCAATAGATAACCAACTTGAATGCCAAGTAAAGGAGTTTGAATCAAAGCAGAAGGAATTTGAATGCCAAAAGAAGGAGTCGATAGTAAGACAAAAATATTTCAAAAGCCGGCTCGAGTCAGGAGAGAAGAAACATGAAAGTCGGTTGAAGGAGCATGAATCAAAAGAAAGAGAGTTTGAAGGCCAAGTGAAGGAGCTGGAATCTAAAAAGAAGCAATTCGAAAGCCAAATGGAGGAGCTCACATCAAATCTGTGGCAATTGAAAGGACAGGTTAAGGAACTTGAGTCCAAAGAGAAGCAGTTTGATAGCCGGGTGAAAGCATTTGAATCAAAAGAGGACGAATTTGAAGGCCGAGCGAAGGAGCATGAATCAAAAGAGAGAGAATTTGAAATTCAAGCGAAGGAGCTGGAATCTAAAAAGAAGCATTTTGAAAGCCAAGTGGAGGAGCTCACATCAAAACTGTGGCAATTGAAAGGACAAGTTAAGGAACTTGAGTACAAAGAGAAGCAATTTGATAGCCGGGTGAAAGCGTTTGAATCAAAAGAGGTTGAATTTAAAGATCGAGTGAGGGAGTTTGAAGCGAAGCAGTTGGAAGTGCAACTGAAGGAGCTAAAGTCAATGCAGAATGAATTTGATGGAGAACTGAAGGAGCTTGAGTTAAGAGAGGATCAATATGAAGCATTACTAAAATCATTTGACGAGGAAATTAAATCAGTTACATGTTACACAGATGATCAATCAACTGCTACCATTGATGGAAGAAGTTTGCAGTTGCTCCCAAGTGACGAAACTGAAATTCTAGATAATCTGCAAGGATTATCAGATCCATCAAAAGTTGTTTTGGATATAATACAGAATCCCATTATTCAAAAGTATAAGATGGGAGACAATGCTGTAATTATTGATGATAGAGACATTCTTCTGCTCAAACAACTGATGAGAATCAAACCACATATTAAACCTCGTGTAAGAGAAGAAGCAATGAAGCTAGCACTTAATTTGAAATCCAACATAAGTGAAAATACTGAAAATTCGGTGGCAGTTCTGGGTTTTCTACTCCTTGTGTCAATTTATGGATTGGCTCCTTCTTTCGATGAAGATGAAGTTTTAAAGCTTTTCGGGTTTGCTGCTCAGGACAAGATAGCTGTAGAGCTGTTTGGAACTCTGGGTTTTGCTGATAAAGCTTCTGATTTTGTTCAGAAACTTATCATGAAGAAACAAAATATTGAAGCTGTTAGATTCATTCGTGCTTATATAATGCCAACAAGAATCAAACCAACGATCTCTTGCATCCAAGAAAATCGTCTCCATTGA
Protein sequence

>Msa0338210

MMNFEAFDKPKFCGNGDDDGRDHVSCEHFESEDDDVPLLPRFTRISSLVKCDVSVKKRDFEFDDNNSLPVSVKKSKVSLYHDHDKDEDDIPLLDLIRRPSKSTDKSFSSLKKGLALLESSFEEGNKKTELEENRLQSIKRDIEECRKELENKKKEISCFRGIDEAHEKLQGKIEECIKDFVVKEGQLYLMDDLIGECKLELKTKEIELNQVKGNISKEIELRQVIDNIDKDRERKEEELKALSQKIAEFALELKAKERELDAMNKLISGKAEKLESKRKKLLKLTSEKENVRAQIKEFESIKKQLEGQVKELESKEKQCDERLEVLISREKQLEGHMKEFESKMQELEGRTMQLESKEKQVEGRAMELKLKEMQLKVQEKEFKSKEEKFEGLMKEVEFKKEHFENQSNVLESIDNQLECQVKEFESKQKEFECQKKESIVRQKYFKSRLESGEKKHESRLKEHESKEREFEGQVKELESKKKQFESQMEELTSNLWQLKGQVKELESKEKQFDSRVKAFESKEDEFEGRAKEHESKEREFEIQAKELESKKKHFESQVEELTSKLWQLKGQVKELEYKEKQFDSRVKAFESKEVEFKDRVREFEAKQLEVQLKELKSMQNEFDGELKELELREDQYEALLKSFDEEIKSVTCYTDDQSTATIDGRSLQLLPSDETEILDNLQGLSDPSKVVLDIIQNPIIQKYKMGDNAVIIDDRDILLLKQLMRIKPHIKPRVREEAMKLALNLKSNISENTENSVAVLGFLLLVSIYGLAPSFDEDEVLKLFGFAAQDKIAVELFGTLGFADKASDFVQKLIMKKQNIEAVRFIRAYIMPTRIKPTISCIQENRLH*