AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0225340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225340 MtrunA17_Chr2g0331691 100.000 209 0 0 1 209 1 209 5.24e-159 437
Msa0225340 MtrunA17_Chr4g0004661 96.098 205 8 0 5 209 59 263 9.71e-151 419
Msa0225340 MtrunA17_Chr4g0034871 85.854 205 28 1 5 209 3 206 2.55e-132 370
Msa0225340 MtrunA17_Chr1g0201121 74.129 201 45 1 6 199 4 204 1.75e-110 315
Msa0225340 MtrunA17_Chr6g0466301 73.529 204 53 1 6 209 4 206 1.78e-110 315
Msa0225340 MtrunA17_Chr6g0465491 73.529 204 53 1 6 209 4 206 1.78e-110 315
Msa0225340 MtrunA17_Chr8g0383261 64.734 207 73 0 1 207 3 209 5.74e-101 291
Msa0225340 MtrunA17_Chr6g0466161 56.284 183 78 2 27 209 7 187 6.95e-69 209
Msa0225340 MtrunA17_Chr6g0465471 56.284 183 78 2 27 209 7 187 1.40e-68 208
Msa0225340 MtrunA17_Chr6g0466431 58.879 107 35 1 6 112 4 101 1.25e-41 136
Msa0225340 MtrunA17_Chr6g0465611 58.879 107 35 1 6 112 4 101 1.25e-41 136
Msa0225340 MtrunA17_Chr2g0276941 36.937 222 116 6 5 209 3 217 1.71e-40 137
Msa0225340 MtrunA17_Chr5g0400131 37.745 204 114 3 12 209 11 207 2.98e-40 136
Msa0225340 MtrunA17_Chr4g0055651 38.308 201 113 5 10 207 8 200 4.69e-40 135
Msa0225340 MtrunA17_Chr2g0276931 36.816 201 117 4 10 207 9 202 1.45e-39 134
Msa0225340 MtrunA17_Chr8g0335471 37.313 201 116 4 10 207 8 201 3.41e-39 133
Msa0225340 MtrunA17_Chr2g0283131 39.086 197 102 6 10 196 13 201 1.47e-38 132
Msa0225340 MtrunA17_Chr7g0250531 37.811 201 114 5 10 207 8 200 1.84e-38 131
Msa0225340 MtrunA17_Chr4g0032201 40.000 165 92 3 10 174 12 169 3.45e-38 131
Msa0225340 MtrunA17_Chr4g0046261 38.071 197 104 6 10 196 13 201 4.97e-38 131
Msa0225340 MtrunA17_Chr4g0040781 41.071 168 91 4 10 176 13 173 8.07e-38 130
Msa0225340 MtrunA17_Chr1g0182321 36.585 205 119 5 10 209 15 213 1.41e-37 130
Msa0225340 MtrunA17_Chr3g0112681 40.000 165 92 3 10 174 12 169 2.01e-37 129
Msa0225340 MtrunA17_Chr1g0182301 36.098 205 120 5 10 209 15 213 4.08e-37 128
Msa0225340 MtrunA17_Chr4g0055081 36.946 203 116 3 12 209 11 206 4.21e-37 128
Msa0225340 MtrunA17_Chr2g0327911 38.378 185 109 4 2 186 7 186 5.86e-37 128
Msa0225340 MtrunA17_Chr3g0123971 37.255 204 114 4 12 209 11 206 7.71e-37 127
Msa0225340 MtrunA17_Chr8g0382221 38.235 204 113 4 12 209 11 207 8.00e-37 127
Msa0225340 MtrunA17_Chr4g0030911 36.585 205 117 5 10 207 15 213 2.05e-36 127
Msa0225340 MtrunA17_Chr1g0212521 35.922 206 118 4 10 207 15 214 5.20e-36 125
Msa0225340 MtrunA17_Chr7g0263071 39.881 168 95 3 10 177 15 176 9.75e-36 125
Msa0225340 MtrunA17_Chr1g0174281 37.037 189 112 3 10 198 15 196 2.82e-35 124
Msa0225340 MtrunA17_Chr7g0214241 36.517 178 106 3 10 187 15 185 5.14e-35 123
Msa0225340 MtrunA17_Chr2g0315551 34.450 209 119 5 10 207 13 214 5.29e-35 123
Msa0225340 MtrunA17_Chr8g0351861 40.741 162 89 3 10 171 16 170 6.97e-35 123
Msa0225340 MtrunA17_Chr2g0325091 39.286 168 94 4 10 176 11 171 2.31e-34 121
Msa0225340 MtrunA17_Chr7g0234201 36.628 172 102 3 10 181 13 177 3.36e-34 121
Msa0225340 MtrunA17_Chr6g0458081 37.209 172 101 3 10 181 6 170 2.46e-33 119
Msa0225340 MtrunA17_Chr2g0319951 38.889 162 92 3 10 171 13 167 3.93e-33 118
Msa0225340 MtrunA17_Chr3g0123821 37.576 165 96 3 10 174 16 173 4.34e-33 118
Msa0225340 MtrunA17_Chr8g0390561 34.783 184 113 3 3 186 2 178 5.12e-33 117
Msa0225340 MtrunA17_Chr6g0482721 39.286 168 94 4 10 176 12 172 7.90e-33 117
Msa0225340 MtrunA17_Chr8g0381841 35.465 172 104 3 3 174 10 174 1.06e-32 117
Msa0225340 MtrunA17_Chr3g0109721 34.595 185 114 3 1 185 1 178 2.25e-32 115
Msa0225340 MtrunA17_Chr7g0231131 38.788 165 94 3 10 174 15 172 2.36e-32 116
Msa0225340 MtrunA17_Chr5g0433561 34.595 185 114 3 1 185 1 178 3.24e-32 115
Msa0225340 MtrunA17_Chr2g0290891 37.654 162 94 3 10 171 6 160 1.49e-31 114
Msa0225340 MtrunA17_Chr1g0189361 36.527 167 99 4 10 176 14 173 2.81e-31 114
Msa0225340 MtrunA17_Chr8g0349711 35.025 197 116 5 7 199 3 191 3.80e-31 113
Msa0225340 MtrunA17_Chr8g0392931 38.889 162 92 3 10 171 14 168 6.27e-31 112
Msa0225340 MtrunA17_Chr7g0275991 37.654 162 94 3 10 171 20 174 8.81e-31 112
Msa0225340 MtrunA17_Chr8g0346421 37.654 162 94 3 10 171 26 180 2.81e-30 111
Msa0225340 MtrunA17_Chr8g0384311 39.024 164 92 5 10 172 13 169 7.02e-30 109
Msa0225340 MtrunA17_Chr8g0349681 39.181 171 93 4 11 177 176 339 1.14e-29 115
Msa0225340 MtrunA17_Chr8g0349681 37.278 169 96 4 3 171 380 538 9.02e-27 107
Msa0225340 MtrunA17_Chr8g0349681 35.503 169 102 4 7 175 10 171 3.22e-26 105
Msa0225340 MtrunA17_Chr1g0199331 34.503 171 104 4 7 176 28 191 7.29e-29 107
Msa0225340 MtrunA17_Chr1g0159481 34.653 202 106 6 10 192 17 211 7.34e-28 104
Msa0225340 MtrunA17_Chr6g0465601 59.211 76 31 0 110 185 3 78 1.60e-27 100
Msa0225340 MtrunA17_Chr7g0265001 38.889 162 92 5 12 172 18 173 2.26e-27 103
Msa0225340 MtrunA17_Chr6g0466421 60.274 73 29 0 113 185 16 88 1.53e-26 97.8
Msa0225340 MtrunA17_Chr1g0197331 37.791 172 99 5 11 180 16 181 1.75e-26 101
Msa0225340 MtrunA17_Chr3g0130271 37.195 164 96 5 10 172 12 169 2.75e-26 100
Msa0225340 MtrunA17_Chr2g0326141 94.118 51 3 0 137 187 305 355 3.53e-26 103
Msa0225340 MtrunA17_Chr1g0180291 38.037 163 93 5 11 172 15 170 3.88e-26 100
Msa0225340 MtrunA17_Chr3g0138121 31.677 161 100 3 11 171 14 164 2.55e-22 90.5
Msa0225340 MtrunA17_Chr3g0138101 31.677 161 100 3 11 171 14 164 2.71e-22 90.5
Msa0225340 MtrunA17_Chr3g0138111 31.677 161 100 3 11 171 14 164 2.80e-22 90.5
Msa0225340 MtrunA17_Chr6g0486041 31.250 208 119 7 10 208 6 198 3.32e-22 89.7
Msa0225340 MtrunA17_Chr2g0325101 30.168 179 110 5 10 179 6 178 6.28e-18 78.2
Msa0225340 MtrunA17_Chr5g0405791 30.168 179 110 6 10 179 6 178 1.76e-17 77.0
Msa0225340 MtrunA17_Chr8g0371501 30.726 179 109 6 10 179 6 178 3.76e-17 76.3
Msa0225340 MtrunA17_Chr3g0117931 30.769 195 117 7 1 186 1 186 4.39e-17 76.3
Msa0225340 MtrunA17_Chr4g0037021 27.957 186 119 4 10 186 6 185 1.28e-16 74.7
Msa0225340 MtrunA17_Chr4g0046211 34.146 123 74 4 10 131 6 122 2.18e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225340 AT1G52280.1 91.262 206 17 1 4 209 2 206 2.81e-142 395
Msa0225340 AT3G16100.1 89.756 205 20 1 5 209 3 206 8.70e-140 389
Msa0225340 AT3G18820.1 88.780 205 22 1 5 209 3 206 1.74e-134 375
Msa0225340 AT1G49300.2 85.854 205 28 1 5 209 3 206 1.03e-130 366
Msa0225340 AT1G49300.1 85.854 205 28 1 5 209 3 206 1.03e-130 366
Msa0225340 AT1G22740.1 73.301 206 49 3 5 209 3 203 1.17e-108 310
Msa0225340 AT4G09720.1 69.231 208 57 2 5 209 3 206 2.05e-106 305
Msa0225340 AT4G09720.3 65.753 219 57 3 5 209 3 217 7.88e-103 296
Msa0225340 AT4G09720.4 67.136 213 58 3 5 209 3 211 8.53e-103 296
Msa0225340 AT2G21880.1 70.556 180 53 0 6 185 5 184 1.43e-93 272
Msa0225340 AT4G09720.6 66.857 175 51 2 38 209 2 172 1.84e-84 248
Msa0225340 AT4G09720.5 66.857 175 51 2 38 209 2 172 1.84e-84 248
Msa0225340 AT4G09720.2 66.857 175 51 2 38 209 2 172 1.84e-84 248
Msa0225340 AT2G21880.2 66.111 180 53 1 6 185 5 176 7.82e-84 247
Msa0225340 AT2G21880.4 70.513 156 46 0 27 182 1 156 3.41e-80 238
Msa0225340 AT2G21880.3 70.513 156 46 0 27 182 1 156 3.41e-80 238
Msa0225340 AT5G39620.1 57.558 172 73 0 11 182 6 177 1.22e-70 214
Msa0225340 AT2G44610.1 38.049 205 113 3 12 209 11 208 5.48e-40 136
Msa0225340 AT1G28550.1 38.384 198 105 5 10 197 13 203 7.70e-40 136
Msa0225340 AT1G09630.1 41.818 165 89 3 10 174 12 169 3.29e-39 134
Msa0225340 AT4G17530.1 38.308 201 113 5 10 207 8 200 6.54e-39 133
Msa0225340 AT5G47200.1 38.308 201 113 5 10 207 8 200 1.05e-38 132
Msa0225340 AT2G33870.1 37.563 197 106 5 10 196 13 202 2.02e-38 132
Msa0225340 AT5G60860.1 39.086 197 102 6 10 196 13 201 3.75e-38 131
Msa0225340 AT3G15060.1 41.975 162 87 3 10 171 13 167 6.16e-38 131
Msa0225340 AT1G02130.1 36.318 201 118 4 10 207 8 201 1.82e-37 129
Msa0225340 AT5G45750.1 36.364 209 114 6 10 207 13 213 2.71e-37 129
Msa0225340 AT5G59840.1 36.893 206 116 5 10 207 15 214 3.18e-37 129
Msa0225340 AT3G53610.2 36.585 205 119 6 10 209 15 213 8.03e-37 128
Msa0225340 AT3G53610.3 36.585 205 119 6 10 209 15 213 8.03e-37 128
Msa0225340 AT3G53610.1 36.585 205 119 6 10 209 15 213 8.03e-37 128
Msa0225340 AT1G05810.1 36.813 182 108 3 6 187 8 182 1.10e-36 128
Msa0225340 AT1G05810.2 36.813 182 108 3 6 187 54 228 2.65e-36 128
Msa0225340 AT3G46060.2 36.408 206 117 5 10 207 15 214 3.08e-36 126
Msa0225340 AT3G46060.3 36.408 206 117 5 10 207 15 214 3.08e-36 126
Msa0225340 AT3G46060.1 36.408 206 117 5 10 207 15 214 3.08e-36 126
Msa0225340 AT5G59150.1 38.182 165 95 3 10 174 12 169 3.49e-36 126
Msa0225340 AT5G64990.1 39.048 210 104 6 12 209 9 206 3.67e-36 126
Msa0225340 AT1G07410.1 38.182 165 95 3 10 174 12 169 9.14e-36 125
Msa0225340 AT3G46830.1 38.788 165 94 3 10 174 12 169 1.09e-35 125
Msa0225340 AT3G46830.2 38.788 165 94 3 10 174 12 169 1.09e-35 125
Msa0225340 AT2G31680.1 35.955 178 107 3 10 187 12 182 1.12e-35 125
Msa0225340 AT3G11730.1 36.139 202 118 4 10 207 8 202 1.25e-35 125
Msa0225340 AT4G18430.1 33.810 210 119 7 10 207 13 214 1.47e-35 125
Msa0225340 AT5G10260.1 36.585 205 115 4 12 209 11 207 3.18e-35 124
Msa0225340 AT3G12160.1 40.606 165 91 3 10 174 15 172 3.88e-35 124
Msa0225340 AT4G18800.1 39.881 168 93 4 10 176 13 173 7.28e-35 123
Msa0225340 AT5G47960.1 34.906 212 117 5 10 207 15 219 1.41e-34 122
Msa0225340 AT2G43130.1 35.955 178 107 3 10 187 12 182 1.91e-34 122
Msa0225340 AT1G06400.1 35.407 209 116 6 10 207 13 213 2.02e-34 122
Msa0225340 AT1G16920.1 39.286 168 94 5 10 176 13 173 3.97e-34 121
Msa0225340 AT4G39890.1 39.634 164 93 2 11 174 10 167 6.37e-34 120
Msa0225340 AT4G35860.1 37.791 172 100 3 10 181 6 170 9.87e-34 120
Msa0225340 AT5G45130.1 34.286 210 123 5 1 207 1 198 1.28e-33 119
Msa0225340 AT4G39990.1 37.576 165 96 3 10 174 17 174 1.32e-33 120
Msa0225340 AT1G18200.1 40.741 162 89 3 10 171 13 167 1.40e-33 120
Msa0225340 AT5G64990.2 37.788 217 104 7 12 209 9 213 1.63e-33 119
Msa0225340 AT5G03520.1 41.071 168 93 3 10 177 15 176 3.69e-33 119
Msa0225340 AT3G07410.1 36.047 172 103 3 10 181 12 176 9.03e-33 117
Msa0225340 AT2G22290.1 35.437 206 116 4 12 209 11 207 1.12e-32 117
Msa0225340 AT5G65270.1 36.364 165 98 3 10 174 17 174 1.76e-32 117
Msa0225340 AT4G19640.1 35.135 185 113 3 1 185 1 178 2.25e-32 116
Msa0225340 AT4G17160.1 38.509 161 92 3 11 171 7 160 2.65e-32 116
Msa0225340 AT3G09900.1 41.667 168 92 4 10 177 15 176 3.28e-32 116
Msa0225340 AT1G73640.1 39.506 162 91 3 10 171 13 167 4.04e-32 116
Msa0225340 AT5G47520.1 36.527 167 99 3 10 176 14 173 7.56e-32 115
Msa0225340 AT4G17170.1 37.654 162 94 3 10 171 6 160 1.57e-31 114
Msa0225340 AT5G45130.2 34.518 197 114 5 14 207 22 206 4.79e-31 113
Msa0225340 AT3G54840.2 35.119 168 101 4 7 173 30 190 9.34e-30 109
Msa0225340 AT1G01200.1 36.420 162 97 2 10 171 28 183 1.10e-29 110
Msa0225340 AT3G54840.1 34.270 178 109 4 7 183 30 200 1.45e-29 109
Msa0225340 AT3G54840.3 34.884 172 104 4 13 183 2 166 7.88e-29 106
Msa0225340 AT1G43890.3 39.024 164 92 5 10 172 13 169 1.89e-28 106
Msa0225340 AT1G43890.1 39.024 164 92 5 10 172 13 169 1.89e-28 106
Msa0225340 AT1G43890.2 39.024 164 92 5 10 172 13 169 1.89e-28 106
Msa0225340 AT5G03530.2 36.082 194 98 7 12 186 15 201 1.55e-27 104
Msa0225340 AT5G03530.1 36.082 194 98 7 12 186 15 201 1.55e-27 104
Msa0225340 AT4G19640.2 36.486 148 89 2 1 148 1 143 1.76e-27 102
Msa0225340 AT4G19640.3 38.168 131 77 1 1 131 1 127 5.29e-26 99.8
Msa0225340 AT5G03520.2 41.667 132 71 3 46 177 41 166 1.01e-24 96.7
Msa0225340 AT5G55190.1 31.677 161 100 3 11 171 14 164 3.44e-22 90.5
Msa0225340 AT5G20010.1 32.530 166 92 5 11 171 14 164 3.70e-22 90.5
Msa0225340 AT5G20020.1 31.677 161 100 3 11 171 14 164 3.94e-22 90.5
Msa0225340 AT3G09910.5 35.465 172 103 5 11 181 14 178 9.49e-22 88.6
Msa0225340 AT3G09910.4 35.465 172 103 5 11 181 14 178 9.49e-22 88.6
Msa0225340 AT5G59150.2 37.398 123 70 3 52 174 3 118 9.93e-22 87.8
Msa0225340 AT3G09910.3 36.196 163 96 5 11 172 14 169 1.46e-21 88.6
Msa0225340 AT3G09910.1 36.196 163 96 5 11 172 14 169 1.46e-21 88.6
Msa0225340 AT3G09910.2 36.196 163 96 5 11 172 14 169 1.46e-21 88.6
Msa0225340 AT4G28950.1 31.068 206 121 7 10 208 6 197 3.14e-21 87.8
Msa0225340 AT4G35860.2 36.923 130 75 3 52 181 2 124 9.37e-21 85.1
Msa0225340 AT5G55080.1 29.814 161 103 3 11 171 14 164 1.04e-20 86.7
Msa0225340 AT5G45970.1 29.444 180 112 5 10 180 6 179 8.01e-18 78.6
Msa0225340 AT5G62880.1 31.746 189 112 6 1 180 1 181 1.96e-17 77.8
Msa0225340 AT3G48040.1 31.746 189 112 6 1 180 1 181 2.53e-17 77.4
Msa0225340 AT4G35020.1 30.000 180 111 5 10 180 6 179 1.04e-16 75.5
Msa0225340 AT4G35020.2 30.000 180 111 5 10 180 6 179 1.04e-16 75.5
Msa0225340 AT4G35020.3 30.000 180 111 5 10 180 6 179 1.04e-16 75.5
Msa0225340 AT1G20090.1 29.050 179 112 5 10 179 5 177 1.58e-16 74.7
Msa0225340 AT2G44690.1 29.651 172 106 5 10 172 18 183 2.05e-16 74.7
Msa0225340 AT1G75840.1 29.050 179 112 5 10 179 6 178 3.07e-16 73.9
Msa0225340 AT3G51300.1 29.609 179 111 5 10 179 6 178 3.65e-16 73.9
Msa0225340 AT2G17800.2 29.050 179 112 5 10 179 6 178 1.93e-15 72.0
Msa0225340 AT2G17800.1 29.050 179 112 5 10 179 6 178 1.93e-15 72.0
Msa0225340 AT4G35950.1 29.050 179 112 5 10 179 6 178 2.50e-15 71.6
Msa0225340 AT5G62880.2 35.606 132 76 5 1 131 1 124 3.75e-15 71.2
Msa0225340 AT3G48040.2 35.606 132 76 5 1 131 1 124 1.05e-14 69.7
Msa0225340 AT5G09910.1 26.012 173 99 5 11 156 23 193 3.89e-13 67.4
Msa0225340 AT5G09910.4 26.012 173 99 5 11 156 23 193 3.89e-13 67.4
Msa0225340 AT4G35020.4 33.058 121 74 4 10 129 6 120 7.61e-13 63.9
Msa0225340 AT4G35020.5 33.058 121 74 4 10 129 6 120 7.61e-13 63.9
Msa0225340 AT5G64813.1 25.758 198 109 7 11 173 23 217 4.93e-12 64.3
Msa0225340 AT5G64813.3 25.758 198 109 7 11 173 23 217 4.93e-12 64.3
Msa0225340 AT5G64813.2 25.758 198 109 7 11 173 23 217 4.93e-12 64.3

Find 65 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACCGACATCAATTGTTTC+TGG 0.232108 2_1:+75472806 None:intergenic
ATTTGAAGGATGCAGTATTT+TGG 0.252931 2_1:+75472701 None:intergenic
GTAATACTAATACATATATA+TGG 0.256785 2_1:+75472516 None:intergenic
AAAACTCACAATTCTTCTTC+AGG 0.319718 2_1:+75473163 None:intergenic
GCGAGAAGAGTTCCTTATTC+AGG 0.320875 2_1:-75473630 Msa0225340:intron
TTGTTTCCCAACACCACAAA+TGG 0.348208 2_1:+75473493 None:intergenic
AAATACTGCATCCTTCAAAT+TGG 0.358102 2_1:-75472698 Msa0225340:three_prime_UTR
ACTCTTCTCGCCAGTGGTTA+AGG 0.372983 2_1:+75473642 None:intergenic
TCCTGAAAACTTCCCATTTG+TGG 0.376974 2_1:-75473506 Msa0225340:CDS
AGACAGGTTGTTCACATTGC+AGG 0.381648 2_1:-75475123 Msa0225340:intron
AAAGTTTAGTAATCAGTATA+AGG 0.387117 2_1:-75475189 Msa0225340:CDS
TTTGCAGAGGTCTCAAAGTA+TGG 0.397953 2_1:+75473247 None:intergenic
AACTTCCCATTTGTGGTGTT+GGG 0.400986 2_1:-75473499 Msa0225340:CDS
GTCTTCAACTTGAACTTCCT+TGG 0.413731 2_1:+75475142 None:intergenic
GTCATCATCCTCGGTGATAG+TGG 0.414536 2_1:-75475397 Msa0225340:CDS
GTCAGCTTCACATGCAAGAT+TGG 0.428919 2_1:-75472726 Msa0225340:three_prime_UTR
GGACGGCAACAGAGGTCAAC+AGG 0.434788 2_1:-75472779 Msa0225340:CDS
ACAATTGATGTCGGTGGCGG+TGG 0.446109 2_1:-75472800 Msa0225340:CDS
CAACTTGAACTTCCTTGGTA+AGG 0.448202 2_1:+75475147 None:intergenic
AAAGGCATGGTGTGCTTCCA+AGG 0.464084 2_1:-75473278 Msa0225340:CDS
GATCTGGGATACTGCTGGTC+AGG 0.467671 2_1:-75473756 Msa0225340:CDS
TAATACTAATACATATATAT+GGG 0.473672 2_1:+75472517 None:intergenic
CAGGAGAGGTTTCAAAGTCT+TGG 0.475629 2_1:-75473737 Msa0225340:CDS
TCCTTCTTGTATCTCTCTAG+TGG 0.482873 2_1:-75472630 Msa0225340:three_prime_UTR
AAACTTCCCATTTGTGGTGT+TGG 0.495054 2_1:-75473500 Msa0225340:CDS
AGAATGTAGCACCAATTTGA+AGG 0.498460 2_1:+75472687 None:intergenic
GGAAACAAAGTAGATGTTGA+TGG 0.512592 2_1:-75473478 Msa0225340:CDS
CTTTGAGACCTCTGCAAAGG+AGG 0.516360 2_1:-75473242 Msa0225340:CDS
AAAGTATGGTATATTCCCCT+TGG 0.522925 2_1:+75473261 None:intergenic
AGGAAGTTCAAGTTGAAGAC+AGG 0.529226 2_1:-75475139 Msa0225340:CDS
GAGAATGAGAAGCCATGGAT+TGG 0.529989 2_1:+75475440 None:intergenic
ACAGTTTCAATTCCAATCCA+TGG 0.530102 2_1:-75475452 Msa0225340:exon
TGTTTCCCAACACCACAAAT+GGG 0.537488 2_1:+75473494 None:intergenic
GTAACATTAAGTAAACAAAC+AGG 0.539021 2_1:+75472595 None:intergenic
TTGATGTCGGTGGCGGTGGA+CGG 0.543988 2_1:-75472796 Msa0225340:CDS
GCGGCGAGAATGAGAAGCCA+TGG 0.559756 2_1:+75475435 None:intergenic
ATGCAGATCTGGGATACTGC+TGG 0.566605 2_1:-75473761 Msa0225340:intron
GAAACAATTGATGTCGGTGG+CGG 0.576334 2_1:-75472803 Msa0225340:CDS
GAGGTTTCAAAGTCTTGGTG+TGG 0.576541 2_1:-75473732 Msa0225340:CDS
CTTGGTGTGGCTTTCTACCG+AGG 0.578330 2_1:-75473719 Msa0225340:CDS
AATCAGTATAAGGCTACCAT+TGG 0.580849 2_1:-75475179 Msa0225340:CDS
TTTGAGACCTCTGCAAAGGA+GGG 0.585589 2_1:-75473241 Msa0225340:CDS
AAGGCATGGTGTGCTTCCAA+GGG 0.586547 2_1:-75473277 Msa0225340:CDS
CAAGAAGGACAAGTTGTCCC+AGG 0.588064 2_1:+75472644 None:intergenic
GGTAAGGAAATCAGCACCAA+TGG 0.599639 2_1:+75475163 None:intergenic
GCCACTAGAGAGATACAAGA+AGG 0.599909 2_1:+75472629 None:intergenic
AACAAAGTAGATGTTGATGG+TGG 0.603728 2_1:-75473475 Msa0225340:CDS
GTGGCGGTGGACGGCAACAG+AGG 0.605632 2_1:-75472787 Msa0225340:CDS
CTGAGAAGAAAGCAAAGGCA+TGG 0.611252 2_1:-75473291 Msa0225340:CDS
TAAGGAACTCTTCTCGCCAG+TGG 0.617763 2_1:+75473636 None:intergenic
TCATCATCCTCGGTGATAGT+GGG 0.618638 2_1:-75475396 Msa0225340:intron
TTATTGAAAGTCATCATCCT+CGG 0.622878 2_1:-75475406 Msa0225340:CDS
CCAGAAACAATTGATGTCGG+TGG 0.629506 2_1:-75472806 Msa0225340:CDS
GGGATACTGCTGGTCAGGAG+AGG 0.630015 2_1:-75473751 Msa0225340:CDS
TGGTGCTGATTTCCTTACCA+AGG 0.632236 2_1:-75475159 Msa0225340:CDS
GGTTTCTGAGAAGAAAGCAA+AGG 0.639361 2_1:-75473296 Msa0225340:CDS
ATTAAAACCCTCCTTTGCAG+AGG 0.646100 2_1:+75473234 None:intergenic
ATACTTTGAGACCTCTGCAA+AGG 0.653339 2_1:-75473245 Msa0225340:CDS
ATGCCAGAAACAATTGATGT+CGG 0.654391 2_1:-75472809 Msa0225340:CDS
TGACATTTACATCATAAACA+AGG 0.673979 2_1:+75473681 None:intergenic
TGATGACTTTCAATAACATG+CGG 0.690006 2_1:+75475413 None:intergenic
GGACACAGCAATCTGCACCT+CGG 0.707086 2_1:+75473702 None:intergenic
AGGCATGGTGTGCTTCCAAG+GGG 0.713588 2_1:-75473276 Msa0225340:CDS
TGACTTTCAATAACATGCGG+CGG 0.715122 2_1:+75475416 None:intergenic
TTATATACCCACTATCACCG+AGG 0.799645 2_1:+75475389 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAATAAATAAATAATTGA+AGG - chr2_1:75473809-75473828 Msa0225340:intron 10.0%
!! TATTTACTTATGAAAAATAA+GGG + chr2_1:75475359-75475378 None:intergenic 10.0%
!! ATATTTACTTATGAAAAATA+AGG + chr2_1:75475360-75475379 None:intergenic 10.0%
!! TAATACTAATACATATATAT+GGG + chr2_1:75475413-75475432 None:intergenic 10.0%
!! TTCATATTACATACATAATT+AGG + chr2_1:75474049-75474068 None:intergenic 15.0%
!! AATTATGTATGTAATATGAA+AGG - chr2_1:75474049-75474068 Msa0225340:intron 15.0%
!! ATTATGTATGTAATATGAAA+GGG - chr2_1:75474050-75474069 Msa0225340:intron 15.0%
!!! CAAAAAAAAAAAAGTTTTGT+TGG - chr2_1:75474851-75474870 Msa0225340:intron 15.0%
!!! AATCTTATTTTAATATAGAG+TGG - chr2_1:75474921-75474940 Msa0225340:intron 15.0%
!! ATTTACTTATGAAAAATAAG+GGG + chr2_1:75475358-75475377 None:intergenic 15.0%
!! GTAATACTAATACATATATA+TGG + chr2_1:75475414-75475433 None:intergenic 15.0%
!!! TTTATTTTTATTTGTTTGGC+AGG - chr2_1:75472677-75472696 Msa0225340:three_prime_UTR 20.0%
!!! TTATTTTTATTTGTTTGGCA+GGG - chr2_1:75472678-75472697 Msa0225340:three_prime_UTR 20.0%
!! AAAGTTTAGTAATCAGTATA+AGG - chr2_1:75472738-75472757 Msa0225340:three_prime_UTR 20.0%
!!! ATATATTTTGACATTGAGAA+TGG + chr2_1:75473255-75473274 None:intergenic 20.0%
!! CTACATGTTAAATGTTAATA+GGG + chr2_1:75473284-75473303 None:intergenic 20.0%
!!! ATTACTATCAGCTTTTAAAT+AGG + chr2_1:75473370-75473389 None:intergenic 20.0%
!!! ACCTAATTGATTTGATTTTT+AGG - chr2_1:75474385-75474404 Msa0225340:intron 20.0%
!!! GAATTCTGAAAAATAATTTG+AGG + chr2_1:75474506-75474525 None:intergenic 20.0%
!!! TTTGATTTACCATATGTTTA+CGG - chr2_1:75474566-75474585 Msa0225340:intron 20.0%
!!! AAAAAAAAAGTTTTGTTGGT+AGG - chr2_1:75474855-75474874 Msa0225340:intron 20.0%
!!! AGGATTTAGAATATTAAAGA+TGG - chr2_1:75474875-75474894 Msa0225340:intron 20.0%
!!! TTTAGAATATTAAAGATGGT+AGG - chr2_1:75474879-75474898 Msa0225340:intron 20.0%
!! TTAGAATATTAAAGATGGTA+GGG - chr2_1:75474880-75474899 Msa0225340:intron 20.0%
!! TTTACTTATGAAAAATAAGG+GGG + chr2_1:75475357-75475376 None:intergenic 20.0%
! AATTGAAAAGGAAAAGGAAA+AGG + chr2_1:75472583-75472602 None:intergenic 25.0%
! AGTTGAAATTGAAAAGGAAA+AGG + chr2_1:75472589-75472608 None:intergenic 25.0%
! TGTGATAGTTGAAATTGAAA+AGG + chr2_1:75472595-75472614 None:intergenic 25.0%
! TTTCAACTATCACATGTTAA+AGG - chr2_1:75472601-75472620 Msa0225340:three_prime_UTR 25.0%
! TTCAACTATCACATGTTAAA+GGG - chr2_1:75472602-75472621 Msa0225340:three_prime_UTR 25.0%
!! AATTAATGTTCGATTCGATT+CGG - chr2_1:75472646-75472665 Msa0225340:three_prime_UTR 25.0%
! GCTACATGTTAAATGTTAAT+AGG + chr2_1:75473285-75473304 None:intergenic 25.0%
! TGATATTCTGACATCAATTT+GGG + chr2_1:75473322-75473341 None:intergenic 25.0%
! AGTTTATACCCAAAAAAACA+GGG + chr2_1:75473410-75473429 None:intergenic 25.0%
! TAGTTTATACCCAAAAAAAC+AGG + chr2_1:75473411-75473430 None:intergenic 25.0%
!! CTTTTGTCTAAGAAAGATAA+AGG + chr2_1:75473619-75473638 None:intergenic 25.0%
!! TATCAAAAAACAAAGTGTCA+TGG + chr2_1:75473868-75473887 None:intergenic 25.0%
!! AAATTCATGTTTCTTTTTCC+TGG - chr2_1:75473962-75473981 Msa0225340:intron 25.0%
!!! AGACCATTGTATTGATTAAA+TGG - chr2_1:75474014-75474033 Msa0225340:intron 25.0%
! GATCCATTTAATCAATACAA+TGG + chr2_1:75474020-75474039 None:intergenic 25.0%
! TGACATTTACATCATAAACA+AGG + chr2_1:75474249-75474268 None:intergenic 25.0%
! GAATAAAAATCACCTGAATA+AGG + chr2_1:75474312-75474331 None:intergenic 25.0%
!! TTCAGGTGATTTTTATTCTT+TGG - chr2_1:75474314-75474333 Msa0225340:intron 25.0%
! ATCTTAACACAATTTCTGAA+GGG - chr2_1:75474346-75474365 Msa0225340:intron 25.0%
! GCCTAAAAATCAAATCAATT+AGG + chr2_1:75474389-75474408 None:intergenic 25.0%
! GAGATAAATAACTTACAACT+CGG + chr2_1:75474484-75474503 None:intergenic 25.0%
! TTGATTTACCATATGTTTAC+GGG - chr2_1:75474567-75474586 Msa0225340:intron 25.0%
! AAAGAGCAAAACTTCTTAAA+TGG + chr2_1:75474831-75474850 None:intergenic 25.0%
! TAGAATATTAAAGATGGTAG+GGG - chr2_1:75474881-75474900 Msa0225340:intron 25.0%
! AGTTATACATTCATTCATAG+CGG + chr2_1:75474986-75475005 None:intergenic 25.0%
!!! GTATAACTTGCTATTTCAAT+TGG - chr2_1:75474998-75475017 Msa0225340:intron 25.0%
! AGGATCAAACAAAATATCTA+TGG + chr2_1:75475076-75475095 None:intergenic 25.0%
!!! ATAGATATTTTGTTTGATCC+TGG - chr2_1:75475075-75475094 Msa0225340:intron 25.0%
! GTAACATTAAGTAAACAAAC+AGG + chr2_1:75475335-75475354 None:intergenic 25.0%
TGATGACTTTCAATAACATG+CGG + chr2_1:75472517-75472536 None:intergenic 30.0%
TTATTGAAAGTCATCATCCT+CGG - chr2_1:75472521-75472540 Msa0225340:three_prime_UTR 30.0%
GACAAATGTTAGTAAGTTAG+CGG - chr2_1:75472865-75472884 Msa0225340:intron 30.0%
CAATATTAAGTGTATGACCT+TGG + chr2_1:75472979-75472998 None:intergenic 30.0%
ACACTGTCGTAAATTGTAAT+CGG + chr2_1:75473172-75473191 None:intergenic 30.0%
GAGAATGGAAAAAAGAAGTA+TGG + chr2_1:75473240-75473259 None:intergenic 30.0%
GTGATATTCTGACATCAATT+TGG + chr2_1:75473323-75473342 None:intergenic 30.0%
! TATCAGCTTTTAAATAGGAC+TGG + chr2_1:75473365-75473384 None:intergenic 30.0%
!!! TTTCATCTACCCTGTTTTTT+TGG - chr2_1:75473398-75473417 Msa0225340:intron 30.0%
!!! TTCATCTACCCTGTTTTTTT+GGG - chr2_1:75473399-75473418 Msa0225340:intron 30.0%
!! ACAAAAGCATCTTCTAACTT+TGG - chr2_1:75473487-75473506 Msa0225340:CDS 30.0%
CTTAGACAAAAGTCAAATGT+TGG - chr2_1:75473627-75473646 Msa0225340:intron 30.0%
! AATTTAATCCTGTGCACTTT+TGG - chr2_1:75473660-75473679 Msa0225340:CDS 30.0%
TCACTACATGATGAAAAACA+AGG - chr2_1:75473700-75473719 Msa0225340:CDS 30.0%
AACAAGGTTTAAGAAGCTAT+TGG - chr2_1:75473716-75473735 Msa0225340:CDS 30.0%
ACAAGGTTTAAGAAGCTATT+GGG - chr2_1:75473717-75473736 Msa0225340:CDS 30.0%
GATATCTCTTCAATGTGATT+TGG + chr2_1:75473762-75473781 None:intergenic 30.0%
GATTCTTGAGCAATATGTTT+GGG + chr2_1:75473784-75473803 None:intergenic 30.0%
AGATTCTTGAGCAATATGTT+TGG + chr2_1:75473785-75473804 None:intergenic 30.0%
TACTTCCTAGATACATATCT+AGG - chr2_1:75473833-75473852 Msa0225340:intron 30.0%
ATATGCCTAGATATGTATCT+AGG + chr2_1:75473841-75473860 None:intergenic 30.0%
GATCTTAACACAATTTCTGA+AGG - chr2_1:75474345-75474364 Msa0225340:intron 30.0%
AGTCTATCAACTACATATGA+TGG + chr2_1:75474528-75474547 None:intergenic 30.0%
TGATTTACCATATGTTTACG+GGG - chr2_1:75474568-75474587 Msa0225340:intron 30.0%
!!! TTCATTCTTGAGAGCATTTT+TGG + chr2_1:75474744-75474763 None:intergenic 30.0%
AAAACTCACAATTCTTCTTC+AGG + chr2_1:75474767-75474786 None:intergenic 30.0%
AGAATATTAAAGATGGTAGG+GGG - chr2_1:75474882-75474901 Msa0225340:intron 30.0%
! ATTTGAAGGATGCAGTATTT+TGG + chr2_1:75475229-75475248 None:intergenic 30.0%
AAATACTGCATCCTTCAAAT+TGG - chr2_1:75475229-75475248 Msa0225340:CDS 30.0%
ACAGTTTCAATTCCAATCCA+TGG - chr2_1:75472475-75472494 Msa0225340:three_prime_UTR 35.0%
AAAGGAAAAGGAAAAGGAGA+AGG + chr2_1:75472577-75472596 None:intergenic 35.0%
!! TTTTATTTGTTTGGCAGGGT+TGG - chr2_1:75472682-75472701 Msa0225340:three_prime_UTR 35.0%
AATCAGTATAAGGCTACCAT+TGG - chr2_1:75472748-75472767 Msa0225340:exon 35.0%
! TAGCGGTTATGTTAGTTTCT+AGG - chr2_1:75472882-75472901 Msa0225340:intron 35.0%
GAAGCTCAATTGGTTAACTA+AGG + chr2_1:75472953-75472972 None:intergenic 35.0%
ATTAAGTGTATGACCTTGGT+AGG + chr2_1:75472975-75472994 None:intergenic 35.0%
GTAATCGGCATCAACTAATT+CGG + chr2_1:75473157-75473176 None:intergenic 35.0%
! TTTAAATAGGACTGGTGAAG+TGG + chr2_1:75473357-75473376 None:intergenic 35.0%
! TTTTGTGACTAGTGTGAACA+AGG + chr2_1:75473473-75473492 None:intergenic 35.0%
GAGACAAGAATGTCAAACAT+TGG + chr2_1:75473583-75473602 None:intergenic 35.0%
ATGTAGTGAGAGGAACAAAA+TGG + chr2_1:75473689-75473708 None:intergenic 35.0%
! GTTTTTCATCATGTAGTGAG+AGG + chr2_1:75473699-75473718 None:intergenic 35.0%
TTGAGATATGATGACATGGT+TGG - chr2_1:75473936-75473955 Msa0225340:intron 35.0%
TGAGATATGATGACATGGTT+GGG - chr2_1:75473937-75473956 Msa0225340:intron 35.0%
!!! AAGCATCGATCTTTTTATCC+AGG + chr2_1:75473983-75474002 None:intergenic 35.0%
!! CTTTTGAAAACCTTAACCAC+TGG - chr2_1:75474275-75474294 Msa0225340:intron 35.0%
! TTAACACAATTTCTGAAGGG+CGG - chr2_1:75474349-75474368 Msa0225340:intron 35.0%
GGAAACAAAGTAGATGTTGA+TGG - chr2_1:75474449-75474468 Msa0225340:intron 35.0%
! AACAAAGTAGATGTTGATGG+TGG - chr2_1:75474452-75474471 Msa0225340:intron 35.0%
GATTTACCATATGTTTACGG+GGG - chr2_1:75474569-75474588 Msa0225340:intron 35.0%
ATTTACCATATGTTTACGGG+GGG - chr2_1:75474570-75474589 Msa0225340:intron 35.0%
AAAGTATGGTATATTCCCCT+TGG + chr2_1:75474669-75474688 None:intergenic 35.0%
!! AAAGGAGGGTTTTAATGTTG+AGG - chr2_1:75474700-75474719 Msa0225340:intron 35.0%
TTTATGCGCATTGTTAACTG+TGG - chr2_1:75474804-75474823 Msa0225340:intron 35.0%
! GTTTCAAGTGATTCTCTTTG+AGG - chr2_1:75475032-75475051 Msa0225340:intron 35.0%
ATCTGTCATAATCAACAACC+AGG + chr2_1:75475096-75475115 None:intergenic 35.0%
ATGCCAGAAACAATTGATGT+CGG - chr2_1:75475118-75475137 Msa0225340:intron 35.0%
AGAATGTAGCACCAATTTGA+AGG + chr2_1:75475243-75475262 None:intergenic 35.0%
! ATTTTCAGCAATGAAAAGCC+TGG - chr2_1:75475265-75475284 Msa0225340:intron 35.0%
! TTTTCAGCAATGAAAAGCCT+GGG - chr2_1:75475266-75475285 Msa0225340:intron 35.0%
TGACTTTCAATAACATGCGG+CGG + chr2_1:75472514-75472533 None:intergenic 40.0%
TTATATACCCACTATCACCG+AGG + chr2_1:75472541-75472560 None:intergenic 40.0%
CAACTTGAACTTCCTTGGTA+AGG + chr2_1:75472783-75472802 None:intergenic 40.0%
GTCTTCAACTTGAACTTCCT+TGG + chr2_1:75472788-75472807 None:intergenic 40.0%
!! AGGAAGTTCAAGTTGAAGAC+AGG - chr2_1:75472788-75472807 Msa0225340:CDS 40.0%
CTGCTTATATCTCCTTCCTT+CGG - chr2_1:75472923-75472942 Msa0225340:intron 40.0%
TCGACGAGACAACACATATA+CGG - chr2_1:75473003-75473022 Msa0225340:intron 40.0%
AGTCTGTGCTTCTTTCGTTA+CGG + chr2_1:75473203-75473222 None:intergenic 40.0%
TTACATGACCCACTTGAAGA+TGG + chr2_1:75473553-75473572 None:intergenic 40.0%
! AATGGATTCCAAAAGTGCAC+AGG + chr2_1:75473671-75473690 None:intergenic 40.0%
! GAAGCTATTGGGCATGTTTT+TGG - chr2_1:75473728-75473747 Msa0225340:CDS 40.0%
GTGCTTGAGATATGATGACA+TGG - chr2_1:75473932-75473951 Msa0225340:intron 40.0%
TCCTGAAAACTTCCCATTTG+TGG - chr2_1:75474421-75474440 Msa0225340:intron 40.0%
! ACCACAAATGGGAAGTTTTC+AGG + chr2_1:75474425-75474444 None:intergenic 40.0%
AAACTTCCCATTTGTGGTGT+TGG - chr2_1:75474427-75474446 Msa0225340:intron 40.0%
AACTTCCCATTTGTGGTGTT+GGG - chr2_1:75474428-75474447 Msa0225340:intron 40.0%
TGTTTCCCAACACCACAAAT+GGG + chr2_1:75474436-75474455 None:intergenic 40.0%
TTGTTTCCCAACACCACAAA+TGG + chr2_1:75474437-75474456 None:intergenic 40.0%
TAGACTTTCTTCCTCTTGAG+AGG - chr2_1:75474542-75474561 Msa0225340:intron 40.0%
AGACTTTCTTCCTCTTGAGA+GGG - chr2_1:75474543-75474562 Msa0225340:intron 40.0%
GTAAATCAAACCCTCTCAAG+AGG + chr2_1:75474556-75474575 None:intergenic 40.0%
TTTACCATATGTTTACGGGG+GGG - chr2_1:75474571-75474590 Msa0225340:intron 40.0%
! GCATTTGTTGCTTCTGTTTC+AGG - chr2_1:75474610-75474629 Msa0225340:intron 40.0%
! GGTTTCTGAGAAGAAAGCAA+AGG - chr2_1:75474631-75474650 Msa0225340:intron 40.0%
TTTGCAGAGGTCTCAAAGTA+TGG + chr2_1:75474683-75474702 None:intergenic 40.0%
!! ATACTTTGAGACCTCTGCAA+AGG - chr2_1:75474682-75474701 Msa0225340:intron 40.0%
ATTAAAACCCTCCTTTGCAG+AGG + chr2_1:75474696-75474715 None:intergenic 40.0%
! TCAAGTGATTCTCTTTGAGG+AGG - chr2_1:75475035-75475054 Msa0225340:intron 40.0%
TCCTTCTTGTATCTCTCTAG+TGG - chr2_1:75475297-75475316 Msa0225340:intron 40.0%
GAGAATGAGAAGCCATGGAT+TGG + chr2_1:75472490-75472509 None:intergenic 45.0%
TCATCATCCTCGGTGATAGT+GGG - chr2_1:75472531-75472550 Msa0225340:three_prime_UTR 45.0%
GGTAAGGAAATCAGCACCAA+TGG + chr2_1:75472767-75472786 None:intergenic 45.0%
!! TGGTGCTGATTTCCTTACCA+AGG - chr2_1:75472768-75472787 Msa0225340:CDS 45.0%
AGACAGGTTGTTCACATTGC+AGG - chr2_1:75472804-75472823 Msa0225340:CDS 45.0%
GAGATATAAGCAGGAGGTCT+AGG + chr2_1:75472916-75472935 None:intergenic 45.0%
AGGAAGGAGATATAAGCAGG+AGG + chr2_1:75472922-75472941 None:intergenic 45.0%
CGAAGGAAGGAGATATAAGC+AGG + chr2_1:75472925-75472944 None:intergenic 45.0%
AACTAAGGAACACCGAAGGA+AGG + chr2_1:75472938-75472957 None:intergenic 45.0%
GGTTAACTAAGGAACACCGA+AGG + chr2_1:75472942-75472961 None:intergenic 45.0%
ACCAATTGAGCTTCCTACCA+AGG - chr2_1:75472959-75472978 Msa0225340:intron 45.0%
ACCTTGGTAGGAAGCTCAAT+TGG + chr2_1:75472963-75472982 None:intergenic 45.0%
CGACGAGACAACACATATAC+GGG - chr2_1:75473004-75473023 Msa0225340:intron 45.0%
GTCTGTGCTTCTTTCGTTAC+GGG + chr2_1:75473202-75473221 None:intergenic 45.0%
! TGGTGGTGAACTTCATGAAC+AGG + chr2_1:75473533-75473552 None:intergenic 45.0%
AGTTCACCACCATCTTCAAG+TGG - chr2_1:75473541-75473560 Msa0225340:intron 45.0%
GTTCACCACCATCTTCAAGT+GGG - chr2_1:75473542-75473561 Msa0225340:intron 45.0%
TCGTTGTGTACATGCAGATC+TGG - chr2_1:75474155-75474174 Msa0225340:intron 45.0%
CGTTGTGTACATGCAGATCT+GGG - chr2_1:75474156-75474175 Msa0225340:intron 45.0%
CAGGAGAGGTTTCAAAGTCT+TGG - chr2_1:75474190-75474209 Msa0225340:intron 45.0%
GAGGTTTCAAAGTCTTGGTG+TGG - chr2_1:75474195-75474214 Msa0225340:intron 45.0%
GCGAGAAGAGTTCCTTATTC+AGG - chr2_1:75474297-75474316 Msa0225340:intron 45.0%
!! GGGAAGTTTTCAGGATCAGA+TGG + chr2_1:75474416-75474435 None:intergenic 45.0%
TTAACCCCCCCGTAAACATA+TGG + chr2_1:75474578-75474597 None:intergenic 45.0%
CTGAGAAGAAAGCAAAGGCA+TGG - chr2_1:75474636-75474655 Msa0225340:intron 45.0%
! TTTGAGACCTCTGCAAAGGA+GGG - chr2_1:75474686-75474705 Msa0225340:intron 45.0%
! GTGATTCTCTTTGAGGAGGA+TGG - chr2_1:75475039-75475058 Msa0225340:intron 45.0%
! TTCTCTTTGAGGAGGATGGT+TGG - chr2_1:75475043-75475062 Msa0225340:intron 45.0%
CCACCGACATCAATTGTTTC+TGG + chr2_1:75475124-75475143 None:intergenic 45.0%
CCAGAAACAATTGATGTCGG+TGG - chr2_1:75475121-75475140 Msa0225340:intron 45.0%
GAAACAATTGATGTCGGTGG+CGG - chr2_1:75475124-75475143 Msa0225340:CDS 45.0%
GTCAGCTTCACATGCAAGAT+TGG - chr2_1:75475201-75475220 Msa0225340:CDS 45.0%
GCCACTAGAGAGATACAAGA+AGG + chr2_1:75475301-75475320 None:intergenic 45.0%
!!! TTTTTTTATTTTTATTTGTT+TGG - chr2_1:75472673-75472692 Msa0225340:three_prime_UTR 5.0%
GTCATCATCCTCGGTGATAG+TGG - chr2_1:75472530-75472549 Msa0225340:three_prime_UTR 50.0%
! TAGGACTGGTGAAGTGGTGA+TGG + chr2_1:75473351-75473370 None:intergenic 50.0%
CATGACCCACTTGAAGATGG+TGG + chr2_1:75473550-75473569 None:intergenic 50.0%
! ATGCAGATCTGGGATACTGC+TGG - chr2_1:75474166-75474185 Msa0225340:intron 50.0%
ACTCTTCTCGCCAGTGGTTA+AGG + chr2_1:75474288-75474307 None:intergenic 50.0%
TAAGGAACTCTTCTCGCCAG+TGG + chr2_1:75474294-75474313 None:intergenic 50.0%
! AAAGGCATGGTGTGCTTCCA+AGG - chr2_1:75474649-75474668 Msa0225340:intron 50.0%
AAGGCATGGTGTGCTTCCAA+GGG - chr2_1:75474650-75474669 Msa0225340:intron 50.0%
!! CTTTGAGACCTCTGCAAAGG+AGG - chr2_1:75474685-75474704 Msa0225340:intron 50.0%
CAAGAAGGACAAGTTGTCCC+AGG + chr2_1:75475286-75475305 None:intergenic 50.0%
! GATCTGGGATACTGCTGGTC+AGG - chr2_1:75474171-75474190 Msa0225340:intron 55.0%
! CTTGGTGTGGCTTTCTACCG+AGG - chr2_1:75474208-75474227 Msa0225340:intron 55.0%
GGACACAGCAATCTGCACCT+CGG + chr2_1:75474228-75474247 None:intergenic 55.0%
AGGCATGGTGTGCTTCCAAG+GGG - chr2_1:75474651-75474670 Msa0225340:intron 55.0%
! ACAATTGATGTCGGTGGCGG+TGG - chr2_1:75475127-75475146 Msa0225340:CDS 55.0%
GCGGCGAGAATGAGAAGCCA+TGG + chr2_1:75472495-75472514 None:intergenic 60.0%
GGGATACTGCTGGTCAGGAG+AGG - chr2_1:75474176-75474195 Msa0225340:intron 60.0%
! TTGATGTCGGTGGCGGTGGA+CGG - chr2_1:75475131-75475150 Msa0225340:CDS 60.0%
GGACGGCAACAGAGGTCAAC+AGG - chr2_1:75475148-75475167 Msa0225340:CDS 60.0%
GTGGCGGTGGACGGCAACAG+AGG - chr2_1:75475140-75475159 Msa0225340:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 75472440 75475509 75472440 ID=Msa0225340;Name=Msa0225340
chr2_1 mRNA 75472440 75475509 75472440 ID=Msa0225340-mRNA-1;Parent=Msa0225340;Name=Msa0225340-mRNA-1;_AED=0.13;_eAED=0.13;_QI=54|1|1|1|1|1|6|326|209
chr2_1 exon 75472440 75472835 75472440 ID=Msa0225340-mRNA-1:exon:22579;Parent=Msa0225340-mRNA-1
chr2_1 exon 75473172 75473317 75473172 ID=Msa0225340-mRNA-1:exon:22578;Parent=Msa0225340-mRNA-1
chr2_1 exon 75473462 75473542 75473462 ID=Msa0225340-mRNA-1:exon:22577;Parent=Msa0225340-mRNA-1
chr2_1 exon 75473631 75473777 75473631 ID=Msa0225340-mRNA-1:exon:22576;Parent=Msa0225340-mRNA-1
chr2_1 exon 75475124 75475250 75475124 ID=Msa0225340-mRNA-1:exon:22575;Parent=Msa0225340-mRNA-1
chr2_1 exon 75475397 75475509 75475397 ID=Msa0225340-mRNA-1:exon:22574;Parent=Msa0225340-mRNA-1
chr2_1 five_prime_UTR 75475456 75475509 75475456 ID=Msa0225340-mRNA-1:five_prime_utr;Parent=Msa0225340-mRNA-1
chr2_1 CDS 75475397 75475455 75475397 ID=Msa0225340-mRNA-1:cds;Parent=Msa0225340-mRNA-1
chr2_1 CDS 75475124 75475250 75475124 ID=Msa0225340-mRNA-1:cds;Parent=Msa0225340-mRNA-1
chr2_1 CDS 75473631 75473777 75473631 ID=Msa0225340-mRNA-1:cds;Parent=Msa0225340-mRNA-1
chr2_1 CDS 75473462 75473542 75473462 ID=Msa0225340-mRNA-1:cds;Parent=Msa0225340-mRNA-1
chr2_1 CDS 75473172 75473317 75473172 ID=Msa0225340-mRNA-1:cds;Parent=Msa0225340-mRNA-1
chr2_1 CDS 75472766 75472835 75472766 ID=Msa0225340-mRNA-1:cds;Parent=Msa0225340-mRNA-1
chr2_1 three_prime_UTR 75472440 75472765 75472440 ID=Msa0225340-mRNA-1:three_prime_utr;Parent=Msa0225340-mRNA-1
Gene Sequence

>Msa0225340

ATGGCTTCTCATTCTCGCCGCCGCATGTTATTGAAAGTCATCATCCTCGGTGATAGTGGGGTTGGCAAAACATCACTCATGAATCAGTATGTGAATCGAAAGTTTAGTAATCAGTATAAGGCTACCATTGGTGCTGATTTCCTTACCAAGGAAGTTCAAGTTGAAGACAGGTTGTTCACATTGCAGATCTGGGATACTGCTGGTCAGGAGAGGTTTCAAAGTCTTGGTGTGGCTTTCTACCGAGGTGCAGATTGCTGTGTCCTTGTTTATGATGTAAATGTCATGAAATCTTTTGAAAACCTTAACCACTGGCGAGAAGAGTTCCTTATTCAGGCTAGTCCATCTGATCCTGAAAACTTCCCATTTGTGGTGTTGGGAAACAAAGTAGATGTTGATGGTGGAAATAGCCGAGTTGTTTCTGAGAAGAAAGCAAAGGCATGGTGTGCTTCCAAGGGGAATATACCATACTTTGAGACCTCTGCAAAGGAGGGTTTTAATGTTGAGGCTGCTTTTGAGTGTATTACCAAAAATGCTCTCAAGAATGAACCTGAAGAAGAATTATACATGCCAGAAACAATTGATGTCGGTGGCGGTGGACGGCAACAGAGGTCAACAGGCTGTGAATGTTGA
Protein sequence

>Msa0225340

MASHSRRRMLLKVIILGDSGVGKTSLMNQYVNRKFSNQYKATIGADFLTKEVQVEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNVMKSFENLNHWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKAKAWCASKGNIPYFETSAKEGFNVEAAFECITKNALKNEPEEELYMPETIDVGGGGRQQRSTGCEC*