AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0822170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0822170 MtrunA17_Chr5g0442391 98.427 572 9 0 1 572 1 572 0.0 1157
Msa0822170 MtrunA17_Chr3g0095501 76.764 581 126 5 1 572 1 581 0.0 872
Msa0822170 MtrunA17_Chr1g0183051 47.400 500 258 3 53 549 7 504 8.33e-163 473
Msa0822170 MtrunA17_Chr3g0095471 56.747 289 110 5 1 282 1 281 2.72e-88 275
Msa0822170 MtrunA17_Chr1g0185501 29.900 301 202 7 253 548 16 312 1.04e-38 144
Msa0822170 MtrunA17_Chr4g0067151 26.948 308 211 7 253 548 16 321 2.20e-29 118
Msa0822170 MtrunA17_Chr4g0067151 27.536 138 96 2 250 384 148 284 2.36e-11 65.5
Msa0822170 MtrunA17_Chr8g0383711 24.242 297 220 5 252 548 6 297 4.09e-24 102
Msa0822170 MtrunA17_Chr7g0253861 22.742 299 219 8 257 547 48 342 3.68e-19 89.4
Msa0822170 MtrunA17_Chr5g0401661 25.305 328 200 10 257 551 328 643 5.41e-17 84.7
Msa0822170 MtrunA17_Chr5g0401661 27.011 174 113 4 257 420 513 682 1.06e-12 70.9
Msa0822170 MtrunA17_Chr1g0176241 27.124 306 185 13 263 552 160 443 1.43e-16 82.8
Msa0822170 MtrunA17_Chr5g0401671 25.382 327 199 10 257 551 240 553 1.50e-16 83.2
Msa0822170 MtrunA17_Chr5g0401671 27.011 174 113 4 257 420 423 592 7.49e-12 68.2
Msa0822170 MtrunA17_Chr5g0401671 23.305 236 153 8 343 559 240 466 2.55e-11 66.2
Msa0822170 MtrunA17_Chr3g0136971 31.214 173 111 5 263 431 460 628 2.28e-16 82.8
Msa0822170 MtrunA17_Chr4g0053651 24.159 327 211 8 252 550 218 535 5.10e-15 78.2
Msa0822170 MtrunA17_Chr4g0053651 28.161 174 111 5 257 420 406 575 8.89e-11 64.7
Msa0822170 MtrunA17_Chr5g0405851 28.095 210 142 6 226 430 255 460 9.95e-14 73.6
Msa0822170 MtrunA17_Chr4g0057141 21.338 314 234 8 243 548 336 644 6.86e-13 71.6
Msa0822170 MtrunA17_Chr1g0185891 21.338 314 234 8 243 548 275 583 7.92e-13 71.2
Msa0822170 MtrunA17_Chr3g0103361 24.390 287 202 9 263 543 18 295 1.06e-12 69.3
Msa0822170 MtrunA17_Chr8g0352821 22.222 297 178 10 259 551 220 467 1.32e-12 70.9
Msa0822170 MtrunA17_Chr7g0266251 23.659 317 179 8 261 552 66 344 2.10e-12 68.6
Msa0822170 MtrunA17_Chr6g0448921 25.000 160 116 3 250 408 87 243 4.11e-12 69.3
Msa0822170 MtrunA17_Chr3g0099061 22.911 371 243 16 208 548 154 511 7.32e-12 67.8
Msa0822170 MtrunA17_Chr1g0172501 29.240 171 114 5 263 430 397 563 1.70e-11 67.0
Msa0822170 MtrunA17_Chr3g0116561 21.908 283 170 9 270 548 273 508 2.11e-11 66.6
Msa0822170 MtrunA17_Chr3g0093681 29.240 171 114 5 263 430 397 563 3.35e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0822170 AT5G08560.1 59.492 590 217 8 1 570 1 588 0.0 712
Msa0822170 AT5G08560.2 59.492 590 217 8 1 570 1 588 0.0 712
Msa0822170 AT5G08560.3 59.492 590 217 8 1 570 1 588 0.0 712
Msa0822170 AT5G43920.1 48.544 515 252 6 53 555 7 520 2.07e-165 481
Msa0822170 AT4G02730.1 32.013 303 194 10 253 548 32 329 3.10e-34 132
Msa0822170 AT3G49660.1 26.974 304 214 5 251 548 11 312 4.39e-30 120
Msa0822170 AT3G49660.1 31.159 138 91 2 250 384 141 277 8.53e-14 73.2
Msa0822170 AT2G43770.1 24.415 299 214 8 257 547 46 340 2.54e-23 102
Msa0822170 AT5G64730.1 24.916 297 218 5 252 548 6 297 1.12e-22 99.0
Msa0822170 AT5G56130.1 25.472 318 211 12 252 552 6 314 2.03e-17 84.0
Msa0822170 AT5G25150.1 36.441 118 71 2 263 379 459 573 3.76e-17 85.5
Msa0822170 AT5G25150.2 36.441 118 71 2 263 379 459 573 3.90e-17 85.5
Msa0822170 AT5G67320.1 24.169 331 206 11 252 550 253 570 1.13e-14 77.4
Msa0822170 AT1G71840.1 21.725 313 199 7 256 565 62 331 1.07e-13 73.6
Msa0822170 AT3G27640.1 24.567 289 171 9 306 550 126 411 5.07e-13 72.0
Msa0822170 AT1G10580.1 22.186 311 229 8 246 548 267 572 5.34e-13 72.0
Msa0822170 AT3G27640.2 24.567 289 171 9 306 550 107 392 6.10e-13 72.0
Msa0822170 AT5G13480.2 21.886 297 179 10 259 551 171 418 1.08e-12 71.2
Msa0822170 AT5G13480.4 21.886 297 179 10 259 551 148 395 1.37e-12 70.9
Msa0822170 AT5G13480.1 21.886 297 179 10 259 551 160 407 1.43e-12 70.9
Msa0822170 AT5G13480.3 21.886 297 179 10 259 551 148 395 1.54e-12 70.9
Msa0822170 AT5G52820.1 23.121 346 208 11 250 548 137 471 1.79e-12 70.1
Msa0822170 AT5G52820.1 26.056 142 104 1 279 420 332 472 7.30e-11 65.1
Msa0822170 AT2G41500.1 22.143 280 166 7 272 548 263 493 3.59e-12 69.3
Msa0822170 AT2G41500.1 23.423 222 124 5 257 435 332 550 1.07e-11 68.2
Msa0822170 AT2G41500.2 22.143 280 166 7 272 548 263 493 3.59e-12 69.3
Msa0822170 AT2G41500.2 23.423 222 124 5 257 435 332 550 1.07e-11 68.2
Msa0822170 AT5G16750.1 30.189 159 101 6 400 554 27 179 5.92e-12 69.3
Msa0822170 AT1G73720.1 20.152 526 361 21 61 548 5 509 5.99e-11 65.5
Msa0822170 AT2G32700.2 22.615 283 199 11 272 548 518 786 8.49e-11 65.5
Msa0822170 AT2G32700.5 22.615 283 199 11 272 548 518 786 8.49e-11 65.5
Msa0822170 AT2G32700.1 22.615 283 199 11 272 548 518 786 8.49e-11 65.5
Msa0822170 AT2G32700.4 22.615 283 199 11 272 548 518 786 8.49e-11 65.5
Msa0822170 AT2G32700.3 22.615 283 199 11 272 548 518 786 8.49e-11 65.5
Msa0822170 AT2G32700.7 22.615 283 199 11 272 548 537 805 8.96e-11 65.5
Msa0822170 AT2G32700.6 22.615 283 199 11 272 548 516 784 9.17e-11 65.5

Find 180 sgRNAs with CRISPR-Local

Find 306 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCCAAAGATTAATTTCT+TGG 0.164363 5_3:-60514114 None:intergenic
CTGTTAAGATTATTAATATA+TGG 0.167131 5_3:+60511396 Msa0822170:five_prime_UTR
AAATTCTTTGAACTTCTAAA+TGG 0.177095 5_3:+60511853 Msa0822170:CDS
ATCGCTTAAGATTTCTGATT+TGG 0.214294 5_3:+60513928 Msa0822170:CDS
AACCCATTTCTTCTGTTTCA+TGG 0.245339 5_3:+60513681 Msa0822170:CDS
AAACAGAAGAAATGGGTTTC+TGG 0.247488 5_3:-60513676 None:intergenic
AGCCCTTACCTTGCAGTTAG+AGG 0.290376 5_3:+60512098 Msa0822170:CDS
AGTGGGAAACATATACTTTC+TGG 0.294428 5_3:+60513836 Msa0822170:CDS
AACAAGTACTTGATGGGAAT+TGG 0.300522 5_3:+60511746 Msa0822170:CDS
TTGAACAGCAATACTGTATT+GGG 0.302367 5_3:-60513992 None:intergenic
ATGACCGTACAATTCGGATA+TGG 0.302803 5_3:+60514729 Msa0822170:CDS
TCATTGGAGTAATAAGAATA+AGG 0.312398 5_3:+60510650 Msa0822170:five_prime_UTR
TGAACCAAGAAATTAATCTT+TGG 0.323203 5_3:+60514110 Msa0822170:CDS
GGCGCTCGAGGTTTATTATA+AGG 0.334980 5_3:+60514179 Msa0822170:CDS
GCAGGGATAGTAGTAGAATT+CGG 0.341628 5_3:+60511929 Msa0822170:CDS
CTTTATCTATATCATTTCAT+TGG 0.341908 5_3:+60510634 Msa0822170:five_prime_UTR
ATTGAACAGCAATACTGTAT+TGG 0.342346 5_3:-60513993 None:intergenic
AGGAGATTACTCCACTTTGC+AGG 0.342931 5_3:+60511911 Msa0822170:CDS
GAGGCACTGCCTGGTCATTC+AGG 0.344214 5_3:+60514647 Msa0822170:CDS
CAATTGCAATGATAATAAAA+AGG 0.346452 5_3:-60514853 None:intergenic
GTCGGCCGCGTGTTTGATAT+TGG 0.346912 5_3:+60511825 Msa0822170:CDS
ATGATCAAACTGCAATCATC+TGG 0.349518 5_3:+60513342 Msa0822170:CDS
CTGGCTTAGTCTCCTGTACA+TGG 0.354065 5_3:+60513807 Msa0822170:CDS
GATTTCTGATTTGGATATAA+CGG 0.356904 5_3:+60513937 Msa0822170:CDS
TTTCTTGATTATTATTCTAC+TGG 0.356996 5_3:-60510550 None:intergenic
ACTCCATGAAACAGAAGAAA+TGG 0.360475 5_3:-60513684 None:intergenic
TAAAGAGAGACGAGTTTGTT+AGG 0.364649 5_3:+60511617 Msa0822170:CDS
TGAAAAGAGGTTGGAACATC+TGG 0.366917 5_3:+60512068 Msa0822170:CDS
GAGTAATAAGAATAAGGTTT+GGG 0.369765 5_3:+60510656 Msa0822170:five_prime_UTR
ATTTGCATGTGGGAATTAGA+TGG 0.371375 5_3:+60513875 Msa0822170:CDS
CTAACCTGTACTGTTCTAGA+TGG 0.373437 5_3:-60512192 None:intergenic
ATAATGTCTCAGGAATTAAA+AGG 0.377105 5_3:-60514982 None:intergenic
AATTCTTTGAACTTCTAAAT+GGG 0.379699 5_3:+60511854 Msa0822170:CDS
GTCTCTCTTTATAACCCCTT+TGG 0.381651 5_3:-60511605 None:intergenic
GTGAGGAGCTCTTCGTCATT+AGG 0.382343 5_3:-60513707 None:intergenic
CAGGGATAGTAGTAGAATTC+GGG 0.384222 5_3:+60511930 Msa0822170:CDS
TTAATGATAAGTGTATATAT+TGG 0.386133 5_3:+60515011 Msa0822170:three_prime_UTR
TTTGAAACGTATGAAGGTTT+CGG 0.391023 5_3:+60511465 Msa0822170:CDS
GCAGATATTGTGTTCCTTTG+AGG 0.393339 5_3:+60511320 Msa0822170:intron
CTTCTTTGTAGGTCTACATA+TGG 0.393962 5_3:+60514600 Msa0822170:intron
GGAGTAATAAGAATAAGGTT+TGG 0.402503 5_3:+60510655 Msa0822170:five_prime_UTR
TGTTAAGATTATTAATATAT+GGG 0.403593 5_3:+60511397 Msa0822170:five_prime_UTR
ATTATAAGGTCTTGCTTTGG+TGG 0.406114 5_3:+60514193 Msa0822170:CDS
GTGGAGTGGAGGAATCTATT+AGG 0.413571 5_3:+60513732 Msa0822170:CDS
GCGCGGCTAGAGGAGGAGTC+TGG 0.418206 5_3:+60511685 Msa0822170:CDS
ATAAATTTGGAGACACTTGC+CGG 0.419366 5_3:-60513771 None:intergenic
TTTATTATAAGGTCTTGCTT+TGG 0.419821 5_3:+60514190 Msa0822170:CDS
CGTGTAAAGCATAGATTATC+TGG 0.420014 5_3:+60513650 Msa0822170:CDS
GCTAACATGTGGGGATTAGC+TGG 0.424867 5_3:-60514695 None:intergenic
TTTACGCAACAAGTACTTGA+TGG 0.428247 5_3:+60511739 Msa0822170:CDS
TTGTACTTCCCAATGAGCTT+AGG 0.432048 5_3:-60514148 None:intergenic
GTAGCTACATTGCGTAAGAT+TGG 0.437473 5_3:+60511778 Msa0822170:CDS
GTTTCGGCGAAAGGATTAGT+TGG 0.438274 5_3:+60511481 Msa0822170:CDS
ACCGTCCGAAGGGGACGATC+AGG 0.440225 5_3:+60511573 Msa0822170:CDS
ACCTTCATACGTTTCAAAGC+TGG 0.443185 5_3:-60511460 None:intergenic
TACATATGGCACCGAAGCTC+AGG 0.443665 5_3:+60514614 Msa0822170:CDS
GATTAGTTGGATTGTCAAAC+GGG 0.445596 5_3:+60511494 Msa0822170:CDS
TTGCCCATTTCACAACTCAT+TGG 0.447546 5_3:+60512122 Msa0822170:CDS
TGATCCAATGAGTTGTGAAA+TGG 0.449737 5_3:-60512126 None:intergenic
TGACCGTACAATTCGGATAT+GGG 0.450477 5_3:+60514730 Msa0822170:CDS
ATTAATCTTTGGAATATTGA+AGG 0.452243 5_3:+60514121 Msa0822170:CDS
ACCTTTACAATATCTTGCTT+AGG 0.458978 5_3:-60511982 None:intergenic
ACAAGTACTTGATGGGAATT+GGG 0.460130 5_3:+60511747 Msa0822170:CDS
GAAGGCGATCCTAAGCTCAT+TGG 0.460407 5_3:+60514139 Msa0822170:CDS
CGTATGAAGGTTTCGGCGAA+AGG 0.461320 5_3:+60511472 Msa0822170:CDS
ATGGGAAAGAAGTGGAGTCT+TGG 0.463584 5_3:+60513894 Msa0822170:CDS
AAGTTCCTCCATTGCAGTAA+TGG 0.463877 5_3:-60514802 None:intergenic
AAGCCTCTAACTGCAAGGTA+AGG 0.469272 5_3:-60512101 None:intergenic
AAGAGAGAAATGATCGGAAT+CGG 0.471535 5_3:-60510797 None:intergenic
GGATTAGTTGGATTGTCAAA+CGG 0.473578 5_3:+60511493 Msa0822170:CDS
CTATCGGCTCTCCTGAGCTT+CGG 0.473900 5_3:-60514625 None:intergenic
AAGGCGATCCTAAGCTCATT+GGG 0.475240 5_3:+60514140 Msa0822170:CDS
GGAGGAATCTATTAGGCGTT+GGG 0.480100 5_3:+60513739 Msa0822170:CDS
CCAGTGATGACCGTACAATT+CGG 0.481345 5_3:+60514723 Msa0822170:CDS
ACCAGCTTTGAAACGTATGA+AGG 0.489309 5_3:+60511459 Msa0822170:CDS
TTGGGAAGTACAAAGGTCAT+AGG 0.494292 5_3:+60514158 Msa0822170:CDS
GGAATACCTTTGCACTCACC+TGG 0.496485 5_3:+60511706 Msa0822170:CDS
ACAATATCTTGCTTAGGAGA+TGG 0.500640 5_3:-60511976 None:intergenic
ATTTGGATATAACGGCTGAC+GGG 0.500965 5_3:+60513945 Msa0822170:CDS
TCAGGTATCATCACTGTTGG+AGG 0.502498 5_3:-60512048 None:intergenic
GCAAAGGCATTGTATTCGCT+TGG 0.504088 5_3:+60511646 Msa0822170:CDS
TCACTTGAGAAACTTCCAAC+AGG 0.504215 5_3:-60511532 None:intergenic
ATGATCGGAATCGGAGTTTG+AGG 0.506112 5_3:-60510788 None:intergenic
AGAAGGTGCGAAGAAGAAGA+AGG 0.507698 5_3:+60510732 Msa0822170:five_prime_UTR
CCGAATTGTACGGTCATCAC+TGG 0.507746 5_3:-60514723 None:intergenic
ATCAGGTTGTCGGTTCCAAA+GGG 0.509238 5_3:+60511590 Msa0822170:CDS
TTCCATGAGTCTAATTCACT+TGG 0.509732 5_3:-60515049 None:intergenic
AGGCGTTGGGATGTTTCTAC+CGG 0.511324 5_3:+60513752 Msa0822170:CDS
TGGAGGAATCTATTAGGCGT+TGG 0.513191 5_3:+60513738 Msa0822170:CDS
GACAACCTGATCGTCCCCTT+CGG 0.518549 5_3:-60511578 None:intergenic
CTCCATGAAACAGAAGAAAT+GGG 0.519066 5_3:-60513683 None:intergenic
GATCCAATGAGTTGTGAAAT+GGG 0.522742 5_3:-60512125 None:intergenic
GATCAGGTTGTCGGTTCCAA+AGG 0.527948 5_3:+60511589 Msa0822170:CDS
TTACGCAACAAGTACTTGAT+GGG 0.533338 5_3:+60511740 Msa0822170:CDS
AAGTTTCTCAAGTGACTCCA+TGG 0.534561 5_3:+60511540 Msa0822170:CDS
GAAAGTATATGTTTCCCACT+TGG 0.535435 5_3:-60513833 None:intergenic
GGAAGCATTGAAGACCTTGA+GGG 0.536828 5_3:+60511888 Msa0822170:CDS
GAAGGGGACGATCAGGTTGT+CGG 0.536973 5_3:+60511580 Msa0822170:CDS
ATTCTTTGAACTTCTAAATG+GGG 0.538918 5_3:+60511855 Msa0822170:CDS
AAGCTCAGGAGAGCCGATAG+AGG 0.539387 5_3:+60514628 Msa0822170:CDS
TGACAGATGACACTATTGTC+AGG 0.541344 5_3:+60511803 Msa0822170:CDS
TTTGCATGTGGGAATTAGAT+GGG 0.543867 5_3:+60513876 Msa0822170:CDS
CAGTGATGATACCTGAAAAG+AGG 0.546109 5_3:+60512055 Msa0822170:CDS
GATTATTAATATATGGGCTG+AGG 0.546954 5_3:+60511403 Msa0822170:five_prime_UTR
GTGATAAGAGCATTTGCATG+TGG 0.550089 5_3:+60513864 Msa0822170:CDS
TTCTTTGAACTTCTAAATGG+GGG 0.550524 5_3:+60511856 Msa0822170:CDS
CTCGGCTACTACCGTCCGAA+GGG 0.555270 5_3:+60511563 Msa0822170:CDS
GATTTGGATATAACGGCTGA+CGG 0.556339 5_3:+60513944 Msa0822170:CDS
GATGATACCTGAAAAGAGGT+TGG 0.556463 5_3:+60512059 Msa0822170:CDS
GAGTGGGGCGCGGCTAGAGG+AGG 0.557951 5_3:+60511678 Msa0822170:CDS
AAAGAGTGGGGCGCGGCTAG+AGG 0.558493 5_3:+60511675 Msa0822170:CDS
GGACGGTAGTAGCCGAGCCA+TGG 0.559268 5_3:-60511557 None:intergenic
CCTGTACATGGTTTCCAAGT+GGG 0.561775 5_3:+60513819 Msa0822170:CDS
GGAGATTACTCCACTTTGCA+GGG 0.564765 5_3:+60511912 Msa0822170:CDS
TCGCTTGGTTATAGAAAGAG+TGG 0.565963 5_3:+60511661 Msa0822170:CDS
GAGCAAGCTTTCGTTGCAAG+TGG 0.568125 5_3:+60514220 Msa0822170:CDS
AAACACATGATGATGAGGTA+TGG 0.568303 5_3:+60513273 Msa0822170:CDS
AAGTGGAAGTGAGGATTCAC+AGG 0.569174 5_3:+60514237 Msa0822170:CDS
GAGCCGATAGAGGCACTGCC+TGG 0.571510 5_3:+60514638 Msa0822170:CDS
CCCACTTGGAAACCATGTAC+AGG 0.572154 5_3:-60513819 None:intergenic
GCTCGGCTACTACCGTCCGA+AGG 0.572516 5_3:+60511562 Msa0822170:CDS
TGATCAAACTGCAATCATCT+GGG 0.573879 5_3:+60513343 Msa0822170:CDS
AGCCTCTAACTGCAAGGTAA+GGG 0.576761 5_3:-60512100 None:intergenic
TCCTAAGCAAGATATTGTAA+AGG 0.577339 5_3:+60511981 Msa0822170:CDS
AACCAAGTGAATTAGACTCA+TGG 0.578587 5_3:+60515047 Msa0822170:three_prime_UTR
GTTTGTTAGGATAATTGCAA+AGG 0.580074 5_3:+60511630 Msa0822170:CDS
ACTGGCAGAGGCTAACATGT+GGG 0.581947 5_3:-60514705 None:intergenic
GATACCATCTAGAACAGTAC+AGG 0.582230 5_3:+60512188 Msa0822170:CDS
TGGAGTAATCTCCTCCCTCA+AGG 0.585707 5_3:-60511902 None:intergenic
TCTTCTTCTAAAGAGCCTGT+TGG 0.587005 5_3:+60511517 Msa0822170:CDS
ATAAAGAAACAAAGGATGAG+AGG 0.587569 5_3:+60514014 Msa0822170:CDS
GGCATCCATTACTGCAATGG+AGG 0.591892 5_3:+60514797 Msa0822170:CDS
GGAATTAGATGGGAAAGAAG+TGG 0.592587 5_3:+60513886 Msa0822170:CDS
TTTCGTTGCAAGTGGAAGTG+AGG 0.593078 5_3:+60514228 Msa0822170:CDS
CGCTTGGTTATAGAAAGAGT+GGG 0.593369 5_3:+60511662 Msa0822170:CDS
TGTACGGTCATCACTGGCAG+AGG 0.595492 5_3:-60514717 None:intergenic
GCTGTTCAATAAAGAAACAA+AGG 0.597283 5_3:+60514006 Msa0822170:CDS
GACGAAGAGCTCCTCACATG+TGG 0.597893 5_3:+60513713 Msa0822170:CDS
GCTCCTCACATGTGGAGTGG+AGG 0.600384 5_3:+60513721 Msa0822170:CDS
ATATTGTAAAGGTGAGATCT+CGG 0.600496 5_3:+60511992 Msa0822170:CDS
TGCCATTGCTGTGCGCATGT+TGG 0.601143 5_3:-60514778 None:intergenic
AATAGAAAGAGAGAAATGAT+CGG 0.602231 5_3:-60510803 None:intergenic
ACCTGATCGTCCCCTTCGGA+CGG 0.603999 5_3:-60511574 None:intergenic
AGGAGATGGAGACACTAAGC+AGG 0.606908 5_3:-60511962 None:intergenic
TCTCAAGTGACTCCATGGCT+CGG 0.607266 5_3:+60511545 Msa0822170:CDS
TTCAGATCATCATTGTGGAA+AGG 0.608588 5_3:+60512161 Msa0822170:CDS
CTTAGAAACACATGATGATG+AGG 0.609621 5_3:+60513268 Msa0822170:CDS
GAGCTGTTAATTGTGTGAGC+TGG 0.609857 5_3:+60514669 Msa0822170:CDS
TTATATTCAGATCATCATTG+TGG 0.610290 5_3:+60512156 Msa0822170:CDS
TGGAAGCATTGAAGACCTTG+AGG 0.611400 5_3:+60511887 Msa0822170:CDS
TGACCAGGCAGTGCCTCTAT+CGG 0.611974 5_3:-60514641 None:intergenic
AATGGCATCCATTACTGCAA+TGG 0.618205 5_3:+60514794 Msa0822170:CDS
GGCCCCATATCCGAATTGTA+CGG 0.618933 5_3:-60514733 None:intergenic
TGATAAGAGCATTTGCATGT+GGG 0.619083 5_3:+60513865 Msa0822170:CDS
ATCTCGGTCAAAGCTTCTAG+AGG 0.620596 5_3:+60512008 Msa0822170:CDS
AAGCTCATTGGGAAGTACAA+AGG 0.624344 5_3:+60514151 Msa0822170:CDS
GACCGTACAATTCGGATATG+GGG 0.628939 5_3:+60514731 Msa0822170:CDS
AGAGCTCCTCACATGTGGAG+TGG 0.631004 5_3:+60513718 Msa0822170:CDS
CACTGGCAGAGGCTAACATG+TGG 0.632424 5_3:-60514706 None:intergenic
CTGGCAGAGGCTAACATGTG+GGG 0.632842 5_3:-60514704 None:intergenic
TTTGGATATAACGGCTGACG+GGG 0.633962 5_3:+60513946 Msa0822170:CDS
ACAAAGGTCATAGGCGCTCG+AGG 0.635818 5_3:+60514167 Msa0822170:CDS
AAAGAAGTGGAGTCTTGGAA+AGG 0.636859 5_3:+60513899 Msa0822170:CDS
TGGGCAAGCCTCTAACTGCA+AGG 0.638184 5_3:-60512106 None:intergenic
TTGACACCAGGTGAGTGCAA+AGG 0.639008 5_3:-60511712 None:intergenic
TCCTGTACATGGTTTCCAAG+TGG 0.639029 5_3:+60513818 Msa0822170:CDS
TTAACAGCTCCTGAATGACC+AGG 0.643672 5_3:-60514656 None:intergenic
AGATGACACTATTGTCAGGT+CGG 0.645072 5_3:+60511807 Msa0822170:CDS
CTACTACTATCCCTGCAAAG+TGG 0.645119 5_3:-60511922 None:intergenic
TACCAACATGCGCACAGCAA+TGG 0.645886 5_3:+60514776 Msa0822170:CDS
ATTCCTCCACTCCACATGTG+AGG 0.658511 5_3:-60513724 None:intergenic
AGCATTGAAGACCTTGAGGG+AGG 0.665437 5_3:+60511891 Msa0822170:CDS
TGCGTAAACAGATTGACACC+AGG 0.666046 5_3:-60511724 None:intergenic
TAACTGAACAGAGAAGAAGA+AGG 0.667440 5_3:+60510715 Msa0822170:five_prime_UTR
CTGTTCCAATATCAAACACG+CGG 0.669040 5_3:-60511830 None:intergenic
GTTATAGAAAGAGTGGGGCG+CGG 0.677569 5_3:+60511668 Msa0822170:CDS
TCGGCTACTACCGTCCGAAG+GGG 0.678925 5_3:+60511564 Msa0822170:CDS
TCAGGTTGTCGGTTCCAAAG+GGG 0.696509 5_3:+60511591 Msa0822170:CDS
GCTTGGTTATAGAAAGAGTG+GGG 0.698893 5_3:+60511663 Msa0822170:CDS
TCAAACTGCAATCATCTGGG+AGG 0.712903 5_3:+60513346 Msa0822170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTAAGATTATTAATATAT+GGG + chr5_3:60511397-60511416 Msa0822170:five_prime_UTR 10.0%
!!! TTTATAGAATTTTTACTATA+AGG - chr5_3:60512955-60512974 None:intergenic 10.0%
!! AGATTAAAAATAATATAGAT+AGG - chr5_3:60513061-60513080 None:intergenic 10.0%
!! AAAGATTAAGATATATGAAT+CGG - chr5_3:60510898-60510917 None:intergenic 15.0%
!! CTGTTAAGATTATTAATATA+TGG + chr5_3:60511396-60511415 Msa0822170:five_prime_UTR 15.0%
!!! AAAGGATAATAAATTTTTGT+TGG + chr5_3:60514078-60514097 Msa0822170:CDS 15.0%
!!! TTTGCAAAATAAAATTCTTT+TGG + chr5_3:60514320-60514339 Msa0822170:intron 15.0%
!! ATTAGATATAAAATGTAGTT+TGG + chr5_3:60514436-60514455 Msa0822170:intron 15.0%
!! TTAATGATAAGTGTATATAT+TGG + chr5_3:60515011-60515030 Msa0822170:three_prime_UTR 15.0%
!! TTTCTTGATTATTATTCTAC+TGG - chr5_3:60510553-60510572 None:intergenic 20.0%
!! CTTTATCTATATCATTTCAT+TGG + chr5_3:60510634-60510653 Msa0822170:five_prime_UTR 20.0%
!!! ATGATTATCTTGATTTTAGA+TGG + chr5_3:60511001-60511020 Msa0822170:intron 20.0%
!!! TTATTTGTTGTTTAAGTTTG+AGG + chr5_3:60511113-60511132 Msa0822170:intron 20.0%
!! AAATTCTTTGAACTTCTAAA+TGG + chr5_3:60511853-60511872 Msa0822170:CDS 20.0%
!! AATTCTTTGAACTTCTAAAT+GGG + chr5_3:60511854-60511873 Msa0822170:CDS 20.0%
!! ATTATGTGGATAATAAATTG+TGG + chr5_3:60512452-60512471 Msa0822170:intron 20.0%
!!! TATCCAATTATGATAGATTT+TGG + chr5_3:60512603-60512622 Msa0822170:intron 20.0%
!! TTTCCAAAATCTATCATAAT+TGG - chr5_3:60512609-60512628 None:intergenic 20.0%
!!! TTTGTACAATTTTCACATAA+TGG + chr5_3:60513296-60513315 Msa0822170:CDS 20.0%
!!! TTGTACAATTTTCACATAAT+GGG + chr5_3:60513297-60513316 Msa0822170:CDS 20.0%
!!! GGGTTTTAGATTTTAATTTT+TGG + chr5_3:60513595-60513614 Msa0822170:intron 20.0%
!!! ATTTTTGGTCTTCTTTTTAA+AGG + chr5_3:60513610-60513629 Msa0822170:intron 20.0%
!! AAGAAAAAGAAGTTATTGTT+TGG - chr5_3:60514054-60514073 None:intergenic 20.0%
!! TATTCCAAAGATTAATTTCT+TGG - chr5_3:60514117-60514136 None:intergenic 20.0%
!!! ATTAATCTTTGGAATATTGA+AGG + chr5_3:60514121-60514140 Msa0822170:CDS 20.0%
!!! AGTAAATATGGATTTTCTAA+AGG - chr5_3:60514381-60514400 None:intergenic 20.0%
!!! TACATTTTATATCTAATCTC+AGG - chr5_3:60514433-60514452 None:intergenic 20.0%
!!! ACTTTTTATTCAGACTATTA+AGG - chr5_3:60514757-60514776 None:intergenic 20.0%
!! CAATTGCAATGATAATAAAA+AGG - chr5_3:60514856-60514875 None:intergenic 20.0%
!! TTAAAAACTAATAATGTCTC+AGG - chr5_3:60514995-60515014 None:intergenic 20.0%
! TCATTGGAGTAATAAGAATA+AGG + chr5_3:60510650-60510669 Msa0822170:five_prime_UTR 25.0%
! GAGTAATAAGAATAAGGTTT+GGG + chr5_3:60510656-60510675 Msa0822170:five_prime_UTR 25.0%
! AATAGAAAGAGAGAAATGAT+CGG - chr5_3:60510806-60510825 None:intergenic 25.0%
! CTGTTATATATCATGTAATC+TGG + chr5_3:60511027-60511046 Msa0822170:intron 25.0%
! TGATACTCATTCATTCTATT+TGG - chr5_3:60511211-60511230 None:intergenic 25.0%
!!! GATGTTTTCGCTTTTATTTA+AGG + chr5_3:60511244-60511263 Msa0822170:intron 25.0%
! ATTCTTTGAACTTCTAAATG+GGG + chr5_3:60511855-60511874 Msa0822170:CDS 25.0%
! TTATATTCAGATCATCATTG+TGG + chr5_3:60512156-60512175 Msa0822170:CDS 25.0%
! GAAATTGCGATATTTATTGA+TGG + chr5_3:60512298-60512317 Msa0822170:intron 25.0%
! AAAATTATGTTGTGATGTAC+TGG + chr5_3:60512429-60512448 Msa0822170:intron 25.0%
! ATCTGAGTTCACTAAATTAA+TGG + chr5_3:60512657-60512676 Msa0822170:intron 25.0%
! AACTAGACAAAATCTTCTAT+TGG + chr5_3:60512686-60512705 Msa0822170:intron 25.0%
!!! GTCACATTTTTTTAATACCA+GGG + chr5_3:60512840-60512859 Msa0822170:intron 25.0%
! TGAAGGAAAAAATTACAACT+AGG - chr5_3:60513474-60513493 None:intergenic 25.0%
!!! AACCAGCTTTTTCATAAATT+TGG - chr5_3:60513787-60513806 None:intergenic 25.0%
!!! GATTTCTGATTTGGATATAA+CGG + chr5_3:60513937-60513956 Msa0822170:CDS 25.0%
!! AACTTCTTTTTCTTTGTCAA+AGG + chr5_3:60514060-60514079 Msa0822170:CDS 25.0%
! TGAACCAAGAAATTAATCTT+TGG + chr5_3:60514110-60514129 Msa0822170:CDS 25.0%
! TTTATTATAAGGTCTTGCTT+TGG + chr5_3:60514190-60514209 Msa0822170:CDS 25.0%
! TACAAGATATTATCCTTCAA+AGG - chr5_3:60514299-60514318 None:intergenic 25.0%
! TTATCACTTTATGTCTTACA+AGG + chr5_3:60514354-60514373 Msa0822170:intron 25.0%
! TATCACTTTATGTCTTACAA+GGG + chr5_3:60514355-60514374 Msa0822170:intron 25.0%
!! ATCCATATTTACTTGTTTCT+AGG + chr5_3:60514388-60514407 Msa0822170:intron 25.0%
! ATAATGTCTCAGGAATTAAA+AGG - chr5_3:60514985-60515004 None:intergenic 25.0%
GGAGTAATAAGAATAAGGTT+TGG + chr5_3:60510655-60510674 Msa0822170:five_prime_UTR 30.0%
TCTCTCTTTCTATTGTAAGT+CGG + chr5_3:60510812-60510831 Msa0822170:intron 30.0%
GCAATCATTCTCAAACTTAT+TGG + chr5_3:60510855-60510874 Msa0822170:intron 30.0%
! GATTATTAATATATGGGCTG+AGG + chr5_3:60511403-60511422 Msa0822170:five_prime_UTR 30.0%
! TTTGAAACGTATGAAGGTTT+CGG + chr5_3:60511465-60511484 Msa0822170:CDS 30.0%
! GTTTGTTAGGATAATTGCAA+AGG + chr5_3:60511630-60511649 Msa0822170:CDS 30.0%
TTCTTTGAACTTCTAAATGG+GGG + chr5_3:60511856-60511875 Msa0822170:CDS 30.0%
TCCTAAGCAAGATATTGTAA+AGG + chr5_3:60511981-60512000 Msa0822170:CDS 30.0%
ACCTTTACAATATCTTGCTT+AGG - chr5_3:60511985-60512004 None:intergenic 30.0%
ATATTGTAAAGGTGAGATCT+CGG + chr5_3:60511992-60512011 Msa0822170:CDS 30.0%
AATGGATGTGATTATTGTTG+AGG + chr5_3:60512484-60512503 Msa0822170:intron 30.0%
AACCAGAAAAATCTAAATCC+CGG - chr5_3:60512511-60512530 None:intergenic 30.0%
ATCACCAACAAGTAGTTAAT+CGG - chr5_3:60512584-60512603 None:intergenic 30.0%
TACTTCACGAATTGAGATTT+GGG - chr5_3:60512801-60512820 None:intergenic 30.0%
TTACTTCACGAATTGAGATT+TGG - chr5_3:60512802-60512821 None:intergenic 30.0%
! GTAACTTTTCTGTCAAAATG+TGG + chr5_3:60512818-60512837 Msa0822170:intron 30.0%
!!! GGTCACATTTTTTTAATACC+AGG + chr5_3:60512839-60512858 Msa0822170:intron 30.0%
GAAAGAAAAATATCAAACCC+TGG - chr5_3:60512860-60512879 None:intergenic 30.0%
!! AGTAACAAGTAGAGTATTGT+CGG - chr5_3:60512892-60512911 None:intergenic 30.0%
GGTTATTGAGTTCTTATTGA+AGG - chr5_3:60512934-60512953 None:intergenic 30.0%
GCATACATACTCATGTAAAT+GGG - chr5_3:60513002-60513021 None:intergenic 30.0%
GACTTCAATTTGATGAATGA+GGG - chr5_3:60513024-60513043 None:intergenic 30.0%
! GCTATATAGAAAGTAATGGA+GGG - chr5_3:60513191-60513210 None:intergenic 30.0%
AGCTATATAGAAAGTAATGG+AGG - chr5_3:60513192-60513211 None:intergenic 30.0%
TCGAGCTATATAGAAAGTAA+TGG - chr5_3:60513195-60513214 None:intergenic 30.0%
!!! CTGTTTTCATGCTTTTTTTC+TGG + chr5_3:60513409-60513428 Msa0822170:intron 30.0%
!! TTGGTCTTCTTTTTAAAGGT+TGG + chr5_3:60513614-60513633 Msa0822170:intron 30.0%
!! TTTTTAAAGGTTGGCGTAAA+TGG + chr5_3:60513623-60513642 Msa0822170:intron 30.0%
CTCCAAATTTATGAAAAAGC+TGG + chr5_3:60513782-60513801 Msa0822170:CDS 30.0%
! ATTTATGAAAAAGCTGGTTC+TGG + chr5_3:60513788-60513807 Msa0822170:CDS 30.0%
ATCGCTTAAGATTTCTGATT+TGG + chr5_3:60513928-60513947 Msa0822170:CDS 30.0%
TTGAACAGCAATACTGTATT+GGG - chr5_3:60513995-60514014 None:intergenic 30.0%
ATTGAACAGCAATACTGTAT+TGG - chr5_3:60513996-60514015 None:intergenic 30.0%
GCTGTTCAATAAAGAAACAA+AGG + chr5_3:60514006-60514025 Msa0822170:CDS 30.0%
ATAAAGAAACAAAGGATGAG+AGG + chr5_3:60514014-60514033 Msa0822170:CDS 30.0%
CACCTAGAAACAAGTAAATA+TGG - chr5_3:60514393-60514412 None:intergenic 30.0%
CTACTATAATCAGAGATGTT+AGG - chr5_3:60514529-60514548 None:intergenic 30.0%
TGATTTCATTCCTTCTTTGT+AGG + chr5_3:60514589-60514608 Msa0822170:intron 30.0%
!! TCTACTTTTGATGACAGTAA+TGG - chr5_3:60514887-60514906 None:intergenic 30.0%
TAACTGAACAGAGAAGAAGA+AGG + chr5_3:60510715-60510734 Msa0822170:five_prime_UTR 35.0%
AAGAGAGAAATGATCGGAAT+CGG - chr5_3:60510800-60510819 None:intergenic 35.0%
!!! TCCTTTGAGGTTTTTTTCAG+AGG + chr5_3:60511333-60511352 Msa0822170:five_prime_UTR 35.0%
! ACCTCTGAAAAAAACCTCAA+AGG - chr5_3:60511337-60511356 None:intergenic 35.0%
!! GGATTAGTTGGATTGTCAAA+CGG + chr5_3:60511493-60511512 Msa0822170:CDS 35.0%
! GATTAGTTGGATTGTCAAAC+GGG + chr5_3:60511494-60511513 Msa0822170:CDS 35.0%
! TAAAGAGAGACGAGTTTGTT+AGG + chr5_3:60511617-60511636 Msa0822170:CDS 35.0%
TTTACGCAACAAGTACTTGA+TGG + chr5_3:60511739-60511758 Msa0822170:CDS 35.0%
TTACGCAACAAGTACTTGAT+GGG + chr5_3:60511740-60511759 Msa0822170:CDS 35.0%
AACAAGTACTTGATGGGAAT+TGG + chr5_3:60511746-60511765 Msa0822170:CDS 35.0%
ACAAGTACTTGATGGGAATT+GGG + chr5_3:60511747-60511766 Msa0822170:CDS 35.0%
ACAATATCTTGCTTAGGAGA+TGG - chr5_3:60511979-60511998 None:intergenic 35.0%
!!! TTTTCAGGTATCATCACTGT+TGG - chr5_3:60512054-60512073 None:intergenic 35.0%
GATCCAATGAGTTGTGAAAT+GGG - chr5_3:60512128-60512147 None:intergenic 35.0%
TGATCCAATGAGTTGTGAAA+TGG - chr5_3:60512129-60512148 None:intergenic 35.0%
TTCAGATCATCATTGTGGAA+AGG + chr5_3:60512161-60512180 Msa0822170:CDS 35.0%
GTATACAATAGTCTCAGCAA+AGG - chr5_3:60512366-60512385 None:intergenic 35.0%
TTGTGATGTACTGGATTATG+TGG + chr5_3:60512438-60512457 Msa0822170:intron 35.0%
AAATTGTGGCATCTTGCAAA+TGG + chr5_3:60512466-60512485 Msa0822170:intron 35.0%
ATGTGATTATTGTTGAGGTC+CGG + chr5_3:60512489-60512508 Msa0822170:intron 35.0%
TATTCCTTTGATGCAAAGAC+AGG - chr5_3:60512534-60512553 None:intergenic 35.0%
TCACCAACAAGTAGTTAATC+GGG - chr5_3:60512583-60512602 None:intergenic 35.0%
! TGATATTTTTCTTTCGCCCT+TGG + chr5_3:60512865-60512884 Msa0822170:intron 35.0%
!! GTAACAAGTAGAGTATTGTC+GGG - chr5_3:60512891-60512910 None:intergenic 35.0%
ATACTCATGTAAATGGGCAT+GGG - chr5_3:60512996-60513015 None:intergenic 35.0%
GGCATACATACTCATGTAAA+TGG - chr5_3:60513003-60513022 None:intergenic 35.0%
GGACTTCAATTTGATGAATG+AGG - chr5_3:60513025-60513044 None:intergenic 35.0%
CGCTGTACTAAATAGCAAAA+GGG - chr5_3:60513163-60513182 None:intergenic 35.0%
TCGCTGTACTAAATAGCAAA+AGG - chr5_3:60513164-60513183 None:intergenic 35.0%
! TATAGAAAGTAATGGAGGGA+AGG - chr5_3:60513187-60513206 None:intergenic 35.0%
! CTTAGAAACACATGATGATG+AGG + chr5_3:60513268-60513287 Msa0822170:CDS 35.0%
! AAACACATGATGATGAGGTA+TGG + chr5_3:60513273-60513292 Msa0822170:CDS 35.0%
ATGATCAAACTGCAATCATC+TGG + chr5_3:60513342-60513361 Msa0822170:CDS 35.0%
TGATCAAACTGCAATCATCT+GGG + chr5_3:60513343-60513362 Msa0822170:CDS 35.0%
!! TTTTGTGCATTGATGAGTGA+GGG - chr5_3:60513512-60513531 None:intergenic 35.0%
!! TTTTTGTGCATTGATGAGTG+AGG - chr5_3:60513513-60513532 None:intergenic 35.0%
! GTGCACTCACATTTAGAATA+TGG + chr5_3:60513555-60513574 Msa0822170:intron 35.0%
CGTGTAAAGCATAGATTATC+TGG + chr5_3:60513650-60513669 Msa0822170:CDS 35.0%
! AAACAGAAGAAATGGGTTTC+TGG - chr5_3:60513679-60513698 None:intergenic 35.0%
AACCCATTTCTTCTGTTTCA+TGG + chr5_3:60513681-60513700 Msa0822170:CDS 35.0%
CTCCATGAAACAGAAGAAAT+GGG - chr5_3:60513686-60513705 None:intergenic 35.0%
ACTCCATGAAACAGAAGAAA+TGG - chr5_3:60513687-60513706 None:intergenic 35.0%
ATAAATTTGGAGACACTTGC+CGG - chr5_3:60513774-60513793 None:intergenic 35.0%
! GAAAGTATATGTTTCCCACT+TGG - chr5_3:60513836-60513855 None:intergenic 35.0%
AGTGGGAAACATATACTTTC+TGG + chr5_3:60513836-60513855 Msa0822170:CDS 35.0%
!! TGATAAGAGCATTTGCATGT+GGG + chr5_3:60513865-60513884 Msa0822170:CDS 35.0%
ATTTGCATGTGGGAATTAGA+TGG + chr5_3:60513875-60513894 Msa0822170:CDS 35.0%
TTTGCATGTGGGAATTAGAT+GGG + chr5_3:60513876-60513895 Msa0822170:CDS 35.0%
ATTATAAGGTCTTGCTTTGG+TGG + chr5_3:60514193-60514212 Msa0822170:CDS 35.0%
GAATGAACACAATCCTTTGA+AGG + chr5_3:60514283-60514302 Msa0822170:intron 35.0%
GCTGTGAATCTTCTTTCATT+TGG + chr5_3:60514477-60514496 Msa0822170:intron 35.0%
TAGTCTCCATTGCTTAAATG+TGG + chr5_3:60514546-60514565 Msa0822170:intron 35.0%
CATTGTCCACATTTAAGCAA+TGG - chr5_3:60514555-60514574 None:intergenic 35.0%
CATATGTAGACCTACAAAGA+AGG - chr5_3:60514602-60514621 None:intergenic 35.0%
CTTCTTTGTAGGTCTACATA+TGG + chr5_3:60514600-60514619 Msa0822170:intron 35.0%
AACCAAGTGAATTAGACTCA+TGG + chr5_3:60515047-60515066 Msa0822170:three_prime_UTR 35.0%
TTCCATGAGTCTAATTCACT+TGG - chr5_3:60515052-60515071 None:intergenic 35.0%
CTCTTTCTATTGTAAGTCGG+TGG + chr5_3:60510815-60510834 Msa0822170:intron 40.0%
GCAGATATTGTGTTCCTTTG+AGG + chr5_3:60511320-60511339 Msa0822170:intron 40.0%
!! ACCAGCTTTGAAACGTATGA+AGG + chr5_3:60511459-60511478 Msa0822170:CDS 40.0%
ACCTTCATACGTTTCAAAGC+TGG - chr5_3:60511463-60511482 None:intergenic 40.0%
! TCTTCTTCTAAAGAGCCTGT+TGG + chr5_3:60511517-60511536 Msa0822170:CDS 40.0%
TCACTTGAGAAACTTCCAAC+AGG - chr5_3:60511535-60511554 None:intergenic 40.0%
AAGTTTCTCAAGTGACTCCA+TGG + chr5_3:60511540-60511559 Msa0822170:CDS 40.0%
GTCTCTCTTTATAACCCCTT+TGG - chr5_3:60511608-60511627 None:intergenic 40.0%
TCGCTTGGTTATAGAAAGAG+TGG + chr5_3:60511661-60511680 Msa0822170:CDS 40.0%
CGCTTGGTTATAGAAAGAGT+GGG + chr5_3:60511662-60511681 Msa0822170:CDS 40.0%
GCTTGGTTATAGAAAGAGTG+GGG + chr5_3:60511663-60511682 Msa0822170:CDS 40.0%
GTAGCTACATTGCGTAAGAT+TGG + chr5_3:60511778-60511797 Msa0822170:CDS 40.0%
TGACAGATGACACTATTGTC+AGG + chr5_3:60511803-60511822 Msa0822170:CDS 40.0%
AGATGACACTATTGTCAGGT+CGG + chr5_3:60511807-60511826 Msa0822170:CDS 40.0%
CTGTTCCAATATCAAACACG+CGG - chr5_3:60511833-60511852 None:intergenic 40.0%
TGAACTTCTAAATGGGGGAA+AGG + chr5_3:60511861-60511880 Msa0822170:CDS 40.0%
TCTAAATGGGGGAAAGGTTA+TGG + chr5_3:60511867-60511886 Msa0822170:CDS 40.0%
GCAGGGATAGTAGTAGAATT+CGG + chr5_3:60511929-60511948 Msa0822170:CDS 40.0%
CAGGGATAGTAGTAGAATTC+GGG + chr5_3:60511930-60511949 Msa0822170:CDS 40.0%
CAGTGATGATACCTGAAAAG+AGG + chr5_3:60512055-60512074 Msa0822170:CDS 40.0%
GATGATACCTGAAAAGAGGT+TGG + chr5_3:60512059-60512078 Msa0822170:CDS 40.0%
! AGATGTTCCAACCTCTTTTC+AGG - chr5_3:60512069-60512088 None:intergenic 40.0%
TGAAAAGAGGTTGGAACATC+TGG + chr5_3:60512068-60512087 Msa0822170:CDS 40.0%
TTGCCCATTTCACAACTCAT+TGG + chr5_3:60512122-60512141 Msa0822170:CDS 40.0%
! GATACCATCTAGAACAGTAC+AGG + chr5_3:60512188-60512207 Msa0822170:CDS 40.0%
! CTAACCTGTACTGTTCTAGA+TGG - chr5_3:60512195-60512214 None:intergenic 40.0%
!! GCTGACTTGTTGATCAGTAA+CGG + chr5_3:60512320-60512339 Msa0822170:intron 40.0%
TGTGATTATTGTTGAGGTCC+GGG + chr5_3:60512490-60512509 Msa0822170:intron 40.0%
! GTCCGGGATTTAGATTTTTC+TGG + chr5_3:60512506-60512525 Msa0822170:intron 40.0%
AACAAGTAGTTAATCGGGGT+TGG - chr5_3:60512578-60512597 None:intergenic 40.0%
AACCCCGATTAACTACTTGT+TGG + chr5_3:60512577-60512596 Msa0822170:intron 40.0%
CACCAACAAGTAGTTAATCG+GGG - chr5_3:60512582-60512601 None:intergenic 40.0%
CATACTCATGTAAATGGGCA+TGG - chr5_3:60512997-60513016 None:intergenic 40.0%
AAAAATCATCCCCAAAAGCC+AGG + chr5_3:60513091-60513110 Msa0822170:intron 40.0%
AAAATCATCCCCAAAAGCCA+GGG + chr5_3:60513092-60513111 Msa0822170:intron 40.0%
AGATCTGTCACAGTATTAGC+AGG - chr5_3:60513250-60513269 None:intergenic 40.0%
ATGGCTTAACTCTGCAACAT+TGG + chr5_3:60513574-60513593 Msa0822170:intron 40.0%
TGGCTTAACTCTGCAACATT+GGG + chr5_3:60513575-60513594 Msa0822170:intron 40.0%
!! GTGATAAGAGCATTTGCATG+TGG + chr5_3:60513864-60513883 Msa0822170:CDS 40.0%
GGAATTAGATGGGAAAGAAG+TGG + chr5_3:60513886-60513905 Msa0822170:CDS 40.0%
AAAGAAGTGGAGTCTTGGAA+AGG + chr5_3:60513899-60513918 Msa0822170:CDS 40.0%
GATTTGGATATAACGGCTGA+CGG + chr5_3:60513944-60513963 Msa0822170:CDS 40.0%
ATTTGGATATAACGGCTGAC+GGG + chr5_3:60513945-60513964 Msa0822170:CDS 40.0%
TTGTACTTCCCAATGAGCTT+AGG - chr5_3:60514151-60514170 None:intergenic 40.0%
! AAGCTCATTGGGAAGTACAA+AGG + chr5_3:60514151-60514170 Msa0822170:CDS 40.0%
! TTGGGAAGTACAAAGGTCAT+AGG + chr5_3:60514158-60514177 Msa0822170:CDS 40.0%
TTCTAGGTGTGATGTTCAGT+AGG + chr5_3:60514404-60514423 Msa0822170:intron 40.0%
! ATCAGAGATGTTAGGTACTG+CGG - chr5_3:60514521-60514540 None:intergenic 40.0%
ATGACCGTACAATTCGGATA+TGG + chr5_3:60514729-60514748 Msa0822170:CDS 40.0%
TGACCGTACAATTCGGATAT+GGG + chr5_3:60514730-60514749 Msa0822170:CDS 40.0%
AATGGCATCCATTACTGCAA+TGG + chr5_3:60514794-60514813 Msa0822170:CDS 40.0%
AAGTTCCTCCATTGCAGTAA+TGG - chr5_3:60514805-60514824 None:intergenic 40.0%
! AGAAGGTGCGAAGAAGAAGA+AGG + chr5_3:60510732-60510751 Msa0822170:five_prime_UTR 45.0%
ATGATCGGAATCGGAGTTTG+AGG - chr5_3:60510791-60510810 None:intergenic 45.0%
! GGCTGAGGTGAACTTTTCAT+GGG + chr5_3:60511418-60511437 Msa0822170:exon 45.0%
! TGAGGTGAACTTTTCATGGG+AGG + chr5_3:60511421-60511440 Msa0822170:exon 45.0%
! TGAACTTTTCATGGGAGGTG+TGG + chr5_3:60511426-60511445 Msa0822170:exon 45.0%
! GTTTCGGCGAAAGGATTAGT+TGG + chr5_3:60511481-60511500 Msa0822170:CDS 45.0%
!! ATCAGGTTGTCGGTTCCAAA+GGG + chr5_3:60511590-60511609 Msa0822170:CDS 45.0%
!! GCAAAGGCATTGTATTCGCT+TGG + chr5_3:60511646-60511665 Msa0822170:CDS 45.0%
TGCGTAAACAGATTGACACC+AGG - chr5_3:60511727-60511746 None:intergenic 45.0%
!! TGGAAGCATTGAAGACCTTG+AGG + chr5_3:60511887-60511906 Msa0822170:CDS 45.0%
!! GGAAGCATTGAAGACCTTGA+GGG + chr5_3:60511888-60511907 Msa0822170:CDS 45.0%
AGGAGATTACTCCACTTTGC+AGG + chr5_3:60511911-60511930 Msa0822170:CDS 45.0%
GGAGATTACTCCACTTTGCA+GGG + chr5_3:60511912-60511931 Msa0822170:CDS 45.0%
CTACTACTATCCCTGCAAAG+TGG - chr5_3:60511925-60511944 None:intergenic 45.0%
ATCTCGGTCAAAGCTTCTAG+AGG + chr5_3:60512008-60512027 Msa0822170:CDS 45.0%
TCAGGTATCATCACTGTTGG+AGG - chr5_3:60512051-60512070 None:intergenic 45.0%
AGCCTCTAACTGCAAGGTAA+GGG - chr5_3:60512103-60512122 None:intergenic 45.0%
AAGCCTCTAACTGCAAGGTA+AGG - chr5_3:60512104-60512123 None:intergenic 45.0%
AAAGGTTCCAGTCTACAAGG+CGG - chr5_3:60512348-60512367 None:intergenic 45.0%
AGCAAAGGTTCCAGTCTACA+AGG - chr5_3:60512351-60512370 None:intergenic 45.0%
GGTTCCTGTCTTTGCATCAA+AGG + chr5_3:60512527-60512546 Msa0822170:intron 45.0%
!! TTTTCTCCTCCCTGGCTTTT+GGG - chr5_3:60513104-60513123 None:intergenic 45.0%
!! ATTTTCTCCTCCCTGGCTTT+TGG - chr5_3:60513105-60513124 None:intergenic 45.0%
! TTTCTCGATTTTCTCCTCCC+TGG - chr5_3:60513112-60513131 None:intergenic 45.0%
GGGAGGAGAAAATCGAGAAA+AGG + chr5_3:60513112-60513131 Msa0822170:intron 45.0%
TCAAACTGCAATCATCTGGG+AGG + chr5_3:60513346-60513365 Msa0822170:CDS 45.0%
GGCATTTGGCTGTGATTTGA+AGG - chr5_3:60513491-60513510 None:intergenic 45.0%
!! CATTGATGAGTGAGGGCATT+TGG - chr5_3:60513505-60513524 None:intergenic 45.0%
GTGGAGTGGAGGAATCTATT+AGG + chr5_3:60513732-60513751 Msa0822170:CDS 45.0%
TGGAGGAATCTATTAGGCGT+TGG + chr5_3:60513738-60513757 Msa0822170:CDS 45.0%
GGAGGAATCTATTAGGCGTT+GGG + chr5_3:60513739-60513758 Msa0822170:CDS 45.0%
TCCTGTACATGGTTTCCAAG+TGG + chr5_3:60513818-60513837 Msa0822170:CDS 45.0%
CCTGTACATGGTTTCCAAGT+GGG + chr5_3:60513819-60513838 Msa0822170:CDS 45.0%
ATGGGAAAGAAGTGGAGTCT+TGG + chr5_3:60513894-60513913 Msa0822170:CDS 45.0%
TTTGGATATAACGGCTGACG+GGG + chr5_3:60513946-60513965 Msa0822170:CDS 45.0%
AAGGCGATCCTAAGCTCATT+GGG + chr5_3:60514140-60514159 Msa0822170:CDS 45.0%
GGCGCTCGAGGTTTATTATA+AGG + chr5_3:60514179-60514198 Msa0822170:CDS 45.0%
TTTCGTTGCAAGTGGAAGTG+AGG + chr5_3:60514228-60514247 Msa0822170:CDS 45.0%
AAGTGGAAGTGAGGATTCAC+AGG + chr5_3:60514237-60514256 Msa0822170:CDS 45.0%
GAAGTGAGGATTCACAGGTA+TGG + chr5_3:60514242-60514261 Msa0822170:intron 45.0%
! AGATGTTAGGTACTGCGGTA+TGG - chr5_3:60514516-60514535 None:intergenic 45.0%
TTAACAGCTCCTGAATGACC+AGG - chr5_3:60514659-60514678 None:intergenic 45.0%
GAGCTGTTAATTGTGTGAGC+TGG + chr5_3:60514669-60514688 Msa0822170:CDS 45.0%
CCAGTGATGACCGTACAATT+CGG + chr5_3:60514723-60514742 Msa0822170:CDS 45.0%
GACCGTACAATTCGGATATG+GGG + chr5_3:60514731-60514750 Msa0822170:CDS 45.0%
! GGGCTGAGGTGAACTTTTCA+TGG + chr5_3:60511417-60511436 Msa0822170:exon 50.0%
CGTATGAAGGTTTCGGCGAA+AGG + chr5_3:60511472-60511491 Msa0822170:CDS 50.0%
TCTCAAGTGACTCCATGGCT+CGG + chr5_3:60511545-60511564 Msa0822170:CDS 50.0%
!! GATCAGGTTGTCGGTTCCAA+AGG + chr5_3:60511589-60511608 Msa0822170:CDS 50.0%
!! TCAGGTTGTCGGTTCCAAAG+GGG + chr5_3:60511591-60511610 Msa0822170:CDS 50.0%
GTTATAGAAAGAGTGGGGCG+CGG + chr5_3:60511668-60511687 Msa0822170:CDS 50.0%
GGAATACCTTTGCACTCACC+TGG + chr5_3:60511706-60511725 Msa0822170:CDS 50.0%
TTGACACCAGGTGAGTGCAA+AGG - chr5_3:60511715-60511734 None:intergenic 50.0%
! AGCATTGAAGACCTTGAGGG+AGG + chr5_3:60511891-60511910 Msa0822170:CDS 50.0%
TGGAGTAATCTCCTCCCTCA+AGG - chr5_3:60511905-60511924 None:intergenic 50.0%
AGGAGATGGAGACACTAAGC+AGG - chr5_3:60511965-60511984 None:intergenic 50.0%
AGCCCTTACCTTGCAGTTAG+AGG + chr5_3:60512098-60512117 Msa0822170:CDS 50.0%
!! GTAGAGTATTGTCGGGCCAA+GGG - chr5_3:60512884-60512903 None:intergenic 50.0%
!! AGTAGAGTATTGTCGGGCCA+AGG - chr5_3:60512885-60512904 None:intergenic 50.0%
GGCATGGGTTTGCATCGAAA+AGG - chr5_3:60512981-60513000 None:intergenic 50.0%
!! TAGATAGGAGCACTGACTCG+TGG - chr5_3:60513046-60513065 None:intergenic 50.0%
!! TTTCTCCTCCCTGGCTTTTG+GGG - chr5_3:60513103-60513122 None:intergenic 50.0%
GTGAGGAGCTCTTCGTCATT+AGG - chr5_3:60513710-60513729 None:intergenic 50.0%
ATTCCTCCACTCCACATGTG+AGG - chr5_3:60513727-60513746 None:intergenic 50.0%
!! AGGCGTTGGGATGTTTCTAC+CGG + chr5_3:60513752-60513771 Msa0822170:CDS 50.0%
CTGGCTTAGTCTCCTGTACA+TGG + chr5_3:60513807-60513826 Msa0822170:CDS 50.0%
CCCACTTGGAAACCATGTAC+AGG - chr5_3:60513822-60513841 None:intergenic 50.0%
GAAGGCGATCCTAAGCTCAT+TGG + chr5_3:60514139-60514158 Msa0822170:CDS 50.0%
GAGCAAGCTTTCGTTGCAAG+TGG + chr5_3:60514220-60514239 Msa0822170:CDS 50.0%
! TACATATGGCACCGAAGCTC+AGG + chr5_3:60514614-60514633 Msa0822170:CDS 50.0%
! GCTAACATGTGGGGATTAGC+TGG - chr5_3:60514698-60514717 None:intergenic 50.0%
ACTGGCAGAGGCTAACATGT+GGG - chr5_3:60514708-60514727 None:intergenic 50.0%
CCGAATTGTACGGTCATCAC+TGG - chr5_3:60514726-60514745 None:intergenic 50.0%
GGCCCCATATCCGAATTGTA+CGG - chr5_3:60514736-60514755 None:intergenic 50.0%
TACCAACATGCGCACAGCAA+TGG + chr5_3:60514776-60514795 Msa0822170:CDS 50.0%
GGCATCCATTACTGCAATGG+AGG + chr5_3:60514797-60514816 Msa0822170:CDS 50.0%
GACAACCTGATCGTCCCCTT+CGG - chr5_3:60511581-60511600 None:intergenic 55.0%
! GAAGGGGACGATCAGGTTGT+CGG + chr5_3:60511580-60511599 Msa0822170:CDS 55.0%
GTCGGCCGCGTGTTTGATAT+TGG + chr5_3:60511825-60511844 Msa0822170:CDS 55.0%
TGGGCAAGCCTCTAACTGCA+AGG - chr5_3:60512109-60512128 None:intergenic 55.0%
ATCATCCCCAAAAGCCAGGG+AGG + chr5_3:60513095-60513114 Msa0822170:intron 55.0%
GACGAAGAGCTCCTCACATG+TGG + chr5_3:60513713-60513732 Msa0822170:CDS 55.0%
AGAGCTCCTCACATGTGGAG+TGG + chr5_3:60513718-60513737 Msa0822170:CDS 55.0%
ACAAAGGTCATAGGCGCTCG+AGG + chr5_3:60514167-60514186 Msa0822170:CDS 55.0%
!! CTATCGGCTCTCCTGAGCTT+CGG - chr5_3:60514628-60514647 None:intergenic 55.0%
AAGCTCAGGAGAGCCGATAG+AGG + chr5_3:60514628-60514647 Msa0822170:CDS 55.0%
TGACCAGGCAGTGCCTCTAT+CGG - chr5_3:60514644-60514663 None:intergenic 55.0%
CTGGCAGAGGCTAACATGTG+GGG - chr5_3:60514707-60514726 None:intergenic 55.0%
CACTGGCAGAGGCTAACATG+TGG - chr5_3:60514709-60514728 None:intergenic 55.0%
TGTACGGTCATCACTGGCAG+AGG - chr5_3:60514720-60514739 None:intergenic 55.0%
TGCCATTGCTGTGCGCATGT+TGG - chr5_3:60514781-60514800 None:intergenic 55.0%
CTCGGCTACTACCGTCCGAA+GGG + chr5_3:60511563-60511582 Msa0822170:CDS 60.0%
TCGGCTACTACCGTCCGAAG+GGG + chr5_3:60511564-60511583 Msa0822170:CDS 60.0%
ACCTGATCGTCCCCTTCGGA+CGG - chr5_3:60511577-60511596 None:intergenic 60.0%
AACGGCACCGCCTTGTAGAC+TGG + chr5_3:60512338-60512357 Msa0822170:intron 60.0%
GCTCCTCACATGTGGAGTGG+AGG + chr5_3:60513721-60513740 Msa0822170:CDS 60.0%
!! GAGGCACTGCCTGGTCATTC+AGG + chr5_3:60514647-60514666 Msa0822170:CDS 60.0%
GGACGGTAGTAGCCGAGCCA+TGG - chr5_3:60511560-60511579 None:intergenic 65.0%
GCTCGGCTACTACCGTCCGA+AGG + chr5_3:60511562-60511581 Msa0822170:CDS 65.0%
ACCGTCCGAAGGGGACGATC+AGG + chr5_3:60511573-60511592 Msa0822170:CDS 65.0%
AAAGAGTGGGGCGCGGCTAG+AGG + chr5_3:60511675-60511694 Msa0822170:CDS 65.0%
!! GAGCCGATAGAGGCACTGCC+TGG + chr5_3:60514638-60514657 Msa0822170:CDS 65.0%
GCGCGGCTAGAGGAGGAGTC+TGG + chr5_3:60511685-60511704 Msa0822170:CDS 70.0%
GAGTGGGGCGCGGCTAGAGG+AGG + chr5_3:60511678-60511697 Msa0822170:CDS 75.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 60510522 60515081 60510522 ID=Msa0822170;Name=Msa0822170
chr5_3 mRNA 60510522 60515081 60510522 ID=Msa0822170-mRNA-1;Parent=Msa0822170;Name=Msa0822170-mRNA-1;_AED=0.01;_eAED=0.01;_QI=416|1|1|1|0.75|0.6|5|255|572
chr5_3 exon 60510522 60510825 60510522 ID=Msa0822170-mRNA-1:exon:18544;Parent=Msa0822170-mRNA-1
chr5_3 exon 60511324 60512209 60511324 ID=Msa0822170-mRNA-1:exon:18545;Parent=Msa0822170-mRNA-1
chr5_3 exon 60513266 60513367 60513266 ID=Msa0822170-mRNA-1:exon:18546;Parent=Msa0822170-mRNA-1
chr5_3 exon 60513632 60514258 60513632 ID=Msa0822170-mRNA-1:exon:18547;Parent=Msa0822170-mRNA-1
chr5_3 exon 60514611 60515081 60514611 ID=Msa0822170-mRNA-1:exon:18548;Parent=Msa0822170-mRNA-1
chr5_3 five_prime_UTR 60510522 60510825 60510522 ID=Msa0822170-mRNA-1:five_prime_utr;Parent=Msa0822170-mRNA-1
chr5_3 five_prime_UTR 60511324 60511435 60511324 ID=Msa0822170-mRNA-1:five_prime_utr;Parent=Msa0822170-mRNA-1
chr5_3 CDS 60511436 60512209 60511436 ID=Msa0822170-mRNA-1:cds;Parent=Msa0822170-mRNA-1
chr5_3 CDS 60513266 60513367 60513266 ID=Msa0822170-mRNA-1:cds;Parent=Msa0822170-mRNA-1
chr5_3 CDS 60513632 60514258 60513632 ID=Msa0822170-mRNA-1:cds;Parent=Msa0822170-mRNA-1
chr5_3 CDS 60514611 60514826 60514611 ID=Msa0822170-mRNA-1:cds;Parent=Msa0822170-mRNA-1
chr5_3 three_prime_UTR 60514827 60515081 60514827 ID=Msa0822170-mRNA-1:three_prime_utr;Parent=Msa0822170-mRNA-1
Gene Sequence

>Msa0822170

ATGGGAGGTGTGGACGATGAAGAACCAGCTTTGAAACGTATGAAGGTTTCGGCGAAAGGATTAGTTGGATTGTCAAACGGGTCTTCTTCTAAAGAGCCTGTTGGAAGTTTCTCAAGTGACTCCATGGCTCGGCTACTACCGTCCGAAGGGGACGATCAGGTTGTCGGTTCCAAAGGGGTTATAAAGAGAGACGAGTTTGTTAGGATAATTGCAAAGGCATTGTATTCGCTTGGTTATAGAAAGAGTGGGGCGCGGCTAGAGGAGGAGTCTGGAATACCTTTGCACTCACCTGGTGTCAATCTGTTTACGCAACAAGTACTTGATGGGAATTGGGATGAGAGTGTAGCTACATTGCGTAAGATTGGTTTGACAGATGACACTATTGTCAGGTCGGCCGCGTGTTTGATATTGGAACAGAAATTCTTTGAACTTCTAAATGGGGGAAAGGTTATGGAAGCATTGAAGACCTTGAGGGAGGAGATTACTCCACTTTGCAGGGATAGTAGTAGAATTCGGGAACTTTCGTCCTGCTTAGTGTCTCCATCTCCTAAGCAAGATATTGTAAAGGTGAGATCTCGGTCAAAGCTTCTAGAGGAATTGCAGAAACTGCTTCCTCCAACAGTGATGATACCTGAAAAGAGGTTGGAACATCTGGTTGAACAAGCCCTTACCTTGCAGTTAGAGGCTTGCCCATTTCACAACTCATTGGATCAGATGTCGTTATATTCAGATCATCATTGTGGAAAGGATCTGATACCATCTAGAACAGTACAGATCTTAGAAACACATGATGATGAGGTATGGTTTGTACAATTTTCACATAATGGGAAGTATTTAGCTTCAGCGTCAAATGATCAAACTGCAATCATCTGGGAGGTTGGCGTAAATGGAGTGCGTGTAAAGCATAGATTATCTGGTCACCAGAAACCCATTTCTTCTGTTTCATGGAGTCCTAATGACGAAGAGCTCCTCACATGTGGAGTGGAGGAATCTATTAGGCGTTGGGATGTTTCTACCGGCAAGTGTCTCCAAATTTATGAAAAAGCTGGTTCTGGCTTAGTCTCCTGTACATGGTTTCCAAGTGGGAAACATATACTTTCTGGCTTAAGTGATAAGAGCATTTGCATGTGGGAATTAGATGGGAAAGAAGTGGAGTCTTGGAAAGGACAAAAATCGCTTAAGATTTCTGATTTGGATATAACGGCTGACGGGGAAGAGATTATAAGTATCTGTAAACCCAATACAGTATTGCTGTTCAATAAAGAAACAAAGGATGAGAGGTTTATTGAAGAGCGCCAAACAATAACTTCTTTTTCTTTGTCAAAGGATAATAAATTTTTGTTGGTTAATCTTTTGAACCAAGAAATTAATCTTTGGAATATTGAAGGCGATCCTAAGCTCATTGGGAAGTACAAAGGTCATAGGCGCTCGAGGTTTATTATAAGGTCTTGCTTTGGTGGACTTGAGCAAGCTTTCGTTGCAAGTGGAAGTGAGGATTCACAGGTCTACATATGGCACCGAAGCTCAGGAGAGCCGATAGAGGCACTGCCTGGTCATTCAGGAGCTGTTAATTGTGTGAGCTGGAATCCAGCTAATCCCCACATGTTAGCCTCTGCCAGTGATGACCGTACAATTCGGATATGGGGCCTTAATAGTCTGAATAAAAAGTACCAACATGCGCACAGCAATGGCATCCATTACTGCAATGGAGGAACTTAG
Protein sequence

>Msa0822170

MGGVDDEEPALKRMKVSAKGLVGLSNGSSSKEPVGSFSSDSMARLLPSEGDDQVVGSKGVIKRDEFVRIIAKALYSLGYRKSGARLEEESGIPLHSPGVNLFTQQVLDGNWDESVATLRKIGLTDDTIVRSAACLILEQKFFELLNGGKVMEALKTLREEITPLCRDSSRIRELSSCLVSPSPKQDIVKVRSRSKLLEELQKLLPPTVMIPEKRLEHLVEQALTLQLEACPFHNSLDQMSLYSDHHCGKDLIPSRTVQILETHDDEVWFVQFSHNGKYLASASNDQTAIIWEVGVNGVRVKHRLSGHQKPISSVSWSPNDEELLTCGVEESIRRWDVSTGKCLQIYEKAGSGLVSCTWFPSGKHILSGLSDKSICMWELDGKEVESWKGQKSLKISDLDITADGEEIISICKPNTVLLFNKETKDERFIEERQTITSFSLSKDNKFLLVNLLNQEINLWNIEGDPKLIGKYKGHRRSRFIIRSCFGGLEQAFVASGSEDSQVYIWHRSSGEPIEALPGHSGAVNCVSWNPANPHMLASASDDRTIRIWGLNSLNKKYQHAHSNGIHYCNGGT*