AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0921870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0921870 MtrunA17_Chr6g0479131 78.571 112 5 2 4 96 1 112 8.50e-53 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTCACCGTTGGGAATTTC+AGG 0.186797 6_2:-59643479 Msa0921870:CDS
GCTTAGGAACTGGTCTTATA+AGG 0.282100 6_2:-59643154 Msa0921870:CDS
CTGAGATAAGACCTGTTCTA+AGG 0.312109 6_2:-59643523 Msa0921870:CDS
CTCAACTTTGAATGGTTTAG+TGG 0.326199 6_2:-59643128 Msa0921870:CDS
TTCATAACTTGACTATGAAT+TGG 0.365682 6_2:+59643593 None:intergenic
TAAGGTTTCTCAACTTTGAA+TGG 0.395755 6_2:-59643136 Msa0921870:CDS
ATATGTTTCATCAAGTTCAT+TGG 0.400281 6_2:-59643181 Msa0921870:intron
CTTAGAACAGGTCTTATCTC+AGG 0.409951 6_2:+59643524 None:intergenic
CAAGTTCATTGGTGTAGCTT+AGG 0.426867 6_2:-59643170 Msa0921870:intron
GGATGAAGGTGTTGGACAAA+AGG 0.436976 6_2:-59643621 Msa0921870:CDS
CGAATTTCGCGGTCACCGTT+GGG 0.446075 6_2:-59643488 Msa0921870:CDS
CGCGAAATCTCCCTTAGAAC+AGG 0.449387 6_2:+59643512 None:intergenic
CATTGGTGTAGCTTAGGAAC+TGG 0.477322 6_2:-59643164 Msa0921870:CDS
AAGAATAACAAGAAGGATGA+AGG 0.505545 6_2:-59643635 Msa0921870:CDS
TCAACTTTGAATGGTTTAGT+GGG 0.510728 6_2:-59643127 Msa0921870:CDS
GATGAAGGTGTTGGACAAAA+GGG 0.511350 6_2:-59643620 Msa0921870:CDS
AAAGTATTTGATTTGTCACC+AGG 0.513116 6_2:-59643551 Msa0921870:CDS
TCAGGCCAAGCTATTTCAGT+TGG 0.530276 6_2:-59643461 Msa0921870:intron
AACAAGAAGGATGAAGGTGT+TGG 0.535489 6_2:-59643629 Msa0921870:CDS
GGTCTTATCTCAGGTTTACC+TGG 0.537252 6_2:+59643533 None:intergenic
AATCACCAACTGAAATAGCT+TGG 0.541732 6_2:+59643456 None:intergenic
TGAGATAAGACCTGTTCTAA+GGG 0.567147 6_2:-59643522 Msa0921870:CDS
AGATTTCGCGACGAATTTCG+CGG 0.567955 6_2:-59643499 Msa0921870:CDS
GGTTTAGTGGGAAAGTTCAA+AGG 0.586118 6_2:-59643115 Msa0921870:CDS
GAACATGAAGAATAACAAGA+AGG 0.590935 6_2:-59643642 Msa0921870:CDS
TAGTGGGAAAGTTCAAAGGA+AGG 0.619708 6_2:-59643111 Msa0921870:CDS
GCTTGGCCTGAAATTCCCAA+CGG 0.707149 6_2:+59643473 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATATATTTTTGTTTTAG+TGG - chr6_2:59643527-59643546 Msa0921870:CDS 10.0%
! TTCATAACTTGACTATGAAT+TGG + chr6_2:59643163-59643182 None:intergenic 25.0%
!! GAATATATCATGTAAGGTTT+TGG - chr6_2:59643330-59643349 Msa0921870:intron 25.0%
! TATCTCTGATTAAAAAACAG+AGG + chr6_2:59643445-59643464 None:intergenic 25.0%
! ATATGTTTCATCAAGTTCAT+TGG - chr6_2:59643572-59643591 Msa0921870:CDS 25.0%
GAACATGAAGAATAACAAGA+AGG - chr6_2:59643111-59643130 Msa0921870:CDS 30.0%
AAGAATAACAAGAAGGATGA+AGG - chr6_2:59643118-59643137 Msa0921870:CDS 30.0%
!! AAAGTATTTGATTTGTCACC+AGG - chr6_2:59643202-59643221 Msa0921870:intron 30.0%
GAGGAAGAATATATCATGTA+AGG - chr6_2:59643324-59643343 Msa0921870:intron 30.0%
! TGATGATGATGTATCATGTA+TGG - chr6_2:59643369-59643388 Msa0921870:intron 30.0%
TAAGGTTTCTCAACTTTGAA+TGG - chr6_2:59643617-59643636 Msa0921870:CDS 30.0%
TCAACTTTGAATGGTTTAGT+GGG - chr6_2:59643626-59643645 Msa0921870:CDS 30.0%
TGAGATAAGACCTGTTCTAA+GGG - chr6_2:59643231-59643250 Msa0921870:intron 35.0%
AATCACCAACTGAAATAGCT+TGG + chr6_2:59643300-59643319 None:intergenic 35.0%
!! TTTCAGTTGGTGATTCATAG+AGG - chr6_2:59643305-59643324 Msa0921870:intron 35.0%
ATCATGTATGGAGATTGTAG+AGG - chr6_2:59643381-59643400 Msa0921870:intron 35.0%
TCATGTATGGAGATTGTAGA+GGG - chr6_2:59643382-59643401 Msa0921870:intron 35.0%
GAAAACAGAGAAAGAAACAG+GGG + chr6_2:59643423-59643442 None:intergenic 35.0%
GGAAAACAGAGAAAGAAACA+GGG + chr6_2:59643424-59643443 None:intergenic 35.0%
AGGAAAACAGAGAAAGAAAC+AGG + chr6_2:59643425-59643444 None:intergenic 35.0%
CTCAACTTTGAATGGTTTAG+TGG - chr6_2:59643625-59643644 Msa0921870:CDS 35.0%
! AACAAGAAGGATGAAGGTGT+TGG - chr6_2:59643124-59643143 Msa0921870:CDS 40.0%
!! GATGAAGGTGTTGGACAAAA+GGG - chr6_2:59643133-59643152 Msa0921870:CDS 40.0%
! CTTAGAACAGGTCTTATCTC+AGG + chr6_2:59643232-59643251 None:intergenic 40.0%
CTGAGATAAGACCTGTTCTA+AGG - chr6_2:59643230-59643249 Msa0921870:intron 40.0%
CAAGTTCATTGGTGTAGCTT+AGG - chr6_2:59643583-59643602 Msa0921870:CDS 40.0%
!! GCTTAGGAACTGGTCTTATA+AGG - chr6_2:59643599-59643618 Msa0921870:CDS 40.0%
GGTTTAGTGGGAAAGTTCAA+AGG - chr6_2:59643638-59643657 Msa0921870:CDS 40.0%
TAGTGGGAAAGTTCAAAGGA+AGG - chr6_2:59643642-59643661 Msa0921870:CDS 40.0%
!! GGATGAAGGTGTTGGACAAA+AGG - chr6_2:59643132-59643151 Msa0921870:CDS 45.0%
! GGTCTTATCTCAGGTTTACC+TGG + chr6_2:59643223-59643242 None:intergenic 45.0%
AGATTTCGCGACGAATTTCG+CGG - chr6_2:59643254-59643273 Msa0921870:intron 45.0%
! TCAGGCCAAGCTATTTCAGT+TGG - chr6_2:59643292-59643311 Msa0921870:intron 45.0%
TGTATGGAGATTGTAGAGGG+AGG - chr6_2:59643385-59643404 Msa0921870:intron 45.0%
CATTGGTGTAGCTTAGGAAC+TGG - chr6_2:59643589-59643608 Msa0921870:CDS 45.0%
CGCGAAATCTCCCTTAGAAC+AGG + chr6_2:59643244-59643263 None:intergenic 50.0%
GCTTGGCCTGAAATTCCCAA+CGG + chr6_2:59643283-59643302 None:intergenic 50.0%
ACGAATTTCGCGGTCACCGT+TGG - chr6_2:59643264-59643283 Msa0921870:intron 55.0%
CGAATTTCGCGGTCACCGTT+GGG - chr6_2:59643265-59643284 Msa0921870:intron 55.0%
CGGTCACCGTTGGGAATTTC+AGG - chr6_2:59643274-59643293 Msa0921870:intron 55.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 59643106 59643669 59643106 ID=Msa0921870;Name=Msa0921870
chr6_2 mRNA 59643106 59643669 59643106 ID=Msa0921870-mRNA-1;Parent=Msa0921870;Name=Msa0921870-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|0|0|2|0|96
chr6_2 exon 59643462 59643669 59643462 ID=Msa0921870-mRNA-1:exon:9123;Parent=Msa0921870-mRNA-1
chr6_2 exon 59643106 59643188 59643106 ID=Msa0921870-mRNA-1:exon:9122;Parent=Msa0921870-mRNA-1
chr6_2 CDS 59643462 59643669 59643462 ID=Msa0921870-mRNA-1:cds;Parent=Msa0921870-mRNA-1
chr6_2 CDS 59643106 59643188 59643106 ID=Msa0921870-mRNA-1:cds;Parent=Msa0921870-mRNA-1
Gene Sequence

>Msa0921870

ATGAAGAACATGAAGAATAACAAGAAGGATGAAGGTGTTGGACAAAAGGGTATACCAATTCATAGTCAAGTTATGAAGATCAAGCAAGAATCAGAGAAAGTATTTGATTTGTCACCAGGTAAACCTGAGATAAGACCTGTTCTAAGGGAGATTTCGCGACGAATTTCGCGGTCACCGTTGGGAATTTCAGGCCAAGCTATTTCAGTTGTTCATTGGTGTAGCTTAGGAACTGGTCTTATAAGGTTTCTCAACTTTGAATGGTTTAGTGGGAAAGTTCAAAGGAAGGACTAG

Protein sequence

>Msa0921870

MKNMKNNKKDEGVGQKGIPIHSQVMKIKQESEKVFDLSPGKPEIRPVLREISRRISRSPLGISGQAISVVHWCSLGTGLIRFLNFEWFSGKVQRKD*