AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0527740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0527740 MtrunA17_Chr2g0321261 90.000 150 15 0 1 150 5 154 3.28e-98 279
Msa0527740 MtrunA17_Chr4g0026561 57.059 170 53 3 1 150 1 170 8.02e-64 192
Msa0527740 MtrunA17_Chr7g0266121 50.595 168 65 4 1 150 1 168 1.27e-51 162
Msa0527740 MtrunA17_Chr1g0178851 48.077 156 75 2 1 150 1 156 6.50e-48 152
Msa0527740 MtrunA17_Chr2g0328941 44.375 160 72 3 8 150 13 172 2.46e-44 143
Msa0527740 MtrunA17_Chr4g0032621 40.000 175 73 4 8 150 13 187 1.94e-40 134
Msa0527740 MtrunA17_Chr1g0173971 40.764 157 79 3 8 150 13 169 1.19e-39 131
Msa0527740 MtrunA17_Chr7g0258071 42.236 161 74 4 8 150 101 260 1.57e-38 131
Msa0527740 MtrunA17_Chr1g0203591 37.333 150 71 3 9 148 2 138 3.03e-31 109
Msa0527740 MtrunA17_Chr7g0274001 38.732 142 75 4 8 148 1 131 3.45e-31 108
Msa0527740 MtrunA17_Chr3g0087831 35.417 144 80 2 12 150 28 163 1.61e-24 92.8
Msa0527740 MtrunA17_Chr2g0306911 37.762 143 70 5 7 148 1 125 1.82e-24 91.7
Msa0527740 MtrunA17_Chr8g0351971 44.792 96 51 2 55 150 147 240 8.01e-22 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0527740 AT2G27580.2 48.571 175 53 2 1 150 1 163 2.93e-53 166
Msa0527740 AT2G27580.1 48.571 175 53 2 1 150 1 163 2.93e-53 166
Msa0527740 AT2G36320.1 49.068 161 71 3 1 150 1 161 1.33e-48 154
Msa0527740 AT3G52800.2 47.647 170 69 4 1 150 1 170 7.83e-44 142
Msa0527740 AT3G52800.1 47.647 170 69 4 1 150 1 170 7.83e-44 142
Msa0527740 AT1G12440.2 44.444 153 73 1 10 150 16 168 8.21e-43 139
Msa0527740 AT1G12440.1 44.444 153 73 1 10 150 16 168 8.21e-43 139
Msa0527740 AT1G12440.3 44.444 153 73 1 10 150 16 168 8.21e-43 139
Msa0527740 AT4G22820.1 42.405 158 74 3 10 150 19 176 8.23e-42 137
Msa0527740 AT4G22820.2 42.405 158 74 3 10 150 19 176 8.23e-42 137
Msa0527740 AT4G12040.2 43.558 163 67 5 10 150 16 175 5.82e-40 132
Msa0527740 AT4G12040.1 43.558 163 67 5 10 150 16 175 5.82e-40 132
Msa0527740 AT1G51200.4 42.683 164 70 4 8 150 13 173 5.29e-38 127
Msa0527740 AT1G51200.1 42.683 164 70 4 8 150 13 173 5.29e-38 127
Msa0527740 AT1G51200.3 42.683 164 70 4 8 150 13 173 5.29e-38 127
Msa0527740 AT1G51200.2 42.683 164 70 4 8 150 13 173 5.29e-38 127
Msa0527740 AT4G25380.1 36.691 139 69 3 13 150 10 130 3.21e-28 101
Msa0527740 AT4G14225.1 36.170 141 70 3 10 150 5 125 5.07e-27 98.2
Msa0527740 AT3G12630.1 37.037 135 78 2 18 150 31 160 7.09e-25 94.0

Find 49 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCACACGCGTGCTGCTC+CGG 0.351983 4_1:-362152 None:intergenic
GTCAACAATTGCGGTTTCTT+CGG 0.368301 4_1:+361840 Msa0527740:CDS
AAGAAGCGCGTGGGGCTGAC+GGG 0.381084 4_1:+362083 Msa0527740:CDS
GTCATCTTCGTTGCCGGATC+CGG 0.388359 4_1:+361998 Msa0527740:CDS
ACCGGCGAGCGTTGCGGTGG+AGG 0.410293 4_1:+362025 Msa0527740:CDS
GGATTGATTGAGAACGAGTT+TGG 0.421778 4_1:-361937 None:intergenic
CTCGCCGGTCCAACCGGATC+CGG 0.425676 4_1:-362011 None:intergenic
TGAACCGTCATCTTCGTTGC+CGG 0.426897 4_1:+361992 Msa0527740:CDS
TCCTCCACCGCAACGCTCGC+CGG 0.433839 4_1:-362026 None:intergenic
AACTTCTCCCCTTTCACCAC+CGG 0.441544 4_1:-362218 None:intergenic
GCAACGCTCGCCGGTCCAAC+CGG 0.447445 4_1:-362017 None:intergenic
TAAGAAGCGCGTGGGGCTGA+CGG 0.457941 4_1:+362082 Msa0527740:CDS
ATCCATCGCAGCGTCTTCTC+CGG 0.463615 4_1:+361956 Msa0527740:CDS
GCAGATTGAGAAAGCGAATC+CGG 0.475883 4_1:+362199 Msa0527740:CDS
ACGCGTGCTGCTCCGGGTAA+CGG 0.484913 4_1:-362145 None:intergenic
AAGCGAATCCGGTGGTGAAA+GGG 0.489320 4_1:+362210 Msa0527740:CDS
CGCCGGAGAAGACGCTGCGA+TGG 0.504990 4_1:-361958 None:intergenic
GTTGCCGGATCCGGTTGGAC+CGG 0.505830 4_1:+362007 Msa0527740:CDS
ACTCACACGCGTGCTGCTCC+GGG 0.506393 4_1:-362151 None:intergenic
CGCAGCGTCTTCTCCGGCGG+TGG 0.510882 4_1:+361962 Msa0527740:CDS
AGAAACCGTTGCAACAGAAT+CGG 0.521190 4_1:+362048 Msa0527740:CDS
GGTGGTGAAAGGGGAGAAGT+TGG 0.528766 4_1:+362220 Msa0527740:CDS
CGATTATGCGTCAACAATTG+CGG 0.530355 4_1:+361831 Msa0527740:CDS
ATGTGGGACCCACCGTTACC+CGG 0.536651 4_1:+362133 Msa0527740:CDS
AAAGCGAATCCGGTGGTGAA+AGG 0.542312 4_1:+362209 Msa0527740:CDS
TCTTCGTTGCCGGATCCGGT+TGG 0.549850 4_1:+362002 Msa0527740:CDS
CGTGCTGCTCCGGGTAACGG+TGG 0.563024 4_1:-362142 None:intergenic
TGGACCGGCGAGCGTTGCGG+TGG 0.567623 4_1:+362022 Msa0527740:CDS
AGAAGCGCGTGGGGCTGACG+GGG 0.576489 4_1:+362084 Msa0527740:CDS
ACGGGGTTTAAGTGCAAGTG+TGG 0.578699 4_1:+362101 Msa0527740:CDS
TTTCTTCGGCAACGCCGCAA+CGG 0.579159 4_1:+361854 Msa0527740:CDS
AGCGAATCCGGTGGTGAAAG+GGG 0.582165 4_1:+362211 Msa0527740:CDS
GGTTGGACCGGCGAGCGTTG+CGG 0.586676 4_1:+362019 Msa0527740:CDS
GTGCTGCTCCGGGTAACGGT+GGG 0.591206 4_1:-362141 None:intergenic
CATCGCAGCGTCTTCTCCGG+CGG 0.604757 4_1:+361959 Msa0527740:CDS
ATACACCGATTCTGTTGCAA+CGG 0.604927 4_1:-362053 None:intergenic
CAATTGTTGACGCATAATCG+TGG 0.606724 4_1:-361828 None:intergenic
ATGACGTGTAAGAAGCGCGT+GGG 0.612329 4_1:+362074 Msa0527740:CDS
GGTTTAAGTGCAAGTGTGGG+AGG 0.629260 4_1:+362105 Msa0527740:CDS
AAGTGTGGGAGGATGTTATG+TGG 0.638134 4_1:+362116 Msa0527740:CDS
CGGGGTTTAAGTGCAAGTGT+GGG 0.646854 4_1:+362102 Msa0527740:CDS
AGTGTGGGAGGATGTTATGT+GGG 0.654340 4_1:+362117 Msa0527740:CDS
TATGACGTGTAAGAAGCGCG+TGG 0.669289 4_1:+362073 Msa0527740:CDS
GGTTCAGAAACAACCACCGC+CGG 0.669731 4_1:-361975 None:intergenic
CTTTGAGCTGTAAATCACGA+TGG 0.672254 4_1:-361896 None:intergenic
TGAACAGAGATTCTCCGTTG+CGG 0.674548 4_1:-361868 None:intergenic
GGATCCGGCAACGAAGATGA+CGG 0.681329 4_1:-361996 None:intergenic
GATTGAGAAAGCGAATCCGG+TGG 0.699709 4_1:+362202 Msa0527740:CDS
TGACGTGTAAGAAGCGCGTG+GGG 0.713127 4_1:+362075 Msa0527740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCGTGTGAGTTTGATTTTAA+GGG + chr4_1:362164-362183 Msa0527740:CDS 35.0%
!! CGTGTGAGTTTGATTTTAAG+GGG + chr4_1:362165-362184 Msa0527740:CDS 35.0%
!! TGAGTTTGATTTTAAGGGGT+TGG + chr4_1:362169-362188 Msa0527740:CDS 35.0%
!! GAGTTTGATTTTAAGGGGTT+GGG + chr4_1:362170-362189 Msa0527740:CDS 35.0%
!! AGTTTGATTTTAAGGGGTTG+GGG + chr4_1:362171-362190 Msa0527740:CDS 35.0%
CAATTGTTGACGCATAATCG+TGG - chr4_1:361831-361850 None:intergenic 40.0%
CGATTATGCGTCAACAATTG+CGG + chr4_1:361831-361850 Msa0527740:CDS 40.0%
GTCAACAATTGCGGTTTCTT+CGG + chr4_1:361840-361859 Msa0527740:CDS 40.0%
CTTTGAGCTGTAAATCACGA+TGG - chr4_1:361899-361918 None:intergenic 40.0%
GGATTGATTGAGAACGAGTT+TGG - chr4_1:361940-361959 None:intergenic 40.0%
AGAAACCGTTGCAACAGAAT+CGG + chr4_1:362048-362067 Msa0527740:CDS 40.0%
!! ATACACCGATTCTGTTGCAA+CGG - chr4_1:362056-362075 None:intergenic 40.0%
!! CGCGTGTGAGTTTGATTTTA+AGG + chr4_1:362163-362182 Msa0527740:CDS 40.0%
TGAACAGAGATTCTCCGTTG+CGG - chr4_1:361871-361890 None:intergenic 45.0%
AAGTGTGGGAGGATGTTATG+TGG + chr4_1:362116-362135 Msa0527740:CDS 45.0%
AGTGTGGGAGGATGTTATGT+GGG + chr4_1:362117-362136 Msa0527740:CDS 45.0%
GCAGATTGAGAAAGCGAATC+CGG + chr4_1:362199-362218 Msa0527740:CDS 45.0%
TGAACCGTCATCTTCGTTGC+CGG + chr4_1:361992-362011 Msa0527740:CDS 50.0%
! TATGACGTGTAAGAAGCGCG+TGG + chr4_1:362073-362092 Msa0527740:CDS 50.0%
! ATGACGTGTAAGAAGCGCGT+GGG + chr4_1:362074-362093 Msa0527740:CDS 50.0%
ACGGGGTTTAAGTGCAAGTG+TGG + chr4_1:362101-362120 Msa0527740:CDS 50.0%
CGGGGTTTAAGTGCAAGTGT+GGG + chr4_1:362102-362121 Msa0527740:CDS 50.0%
GGTTTAAGTGCAAGTGTGGG+AGG + chr4_1:362105-362124 Msa0527740:CDS 50.0%
GATTGAGAAAGCGAATCCGG+TGG + chr4_1:362202-362221 Msa0527740:CDS 50.0%
AAAGCGAATCCGGTGGTGAA+AGG + chr4_1:362209-362228 Msa0527740:CDS 50.0%
AAGCGAATCCGGTGGTGAAA+GGG + chr4_1:362210-362229 Msa0527740:CDS 50.0%
AACTTCTCCCCTTTCACCAC+CGG - chr4_1:362221-362240 None:intergenic 50.0%
TTTCTTCGGCAACGCCGCAA+CGG + chr4_1:361854-361873 Msa0527740:CDS 55.0%
ATCCATCGCAGCGTCTTCTC+CGG + chr4_1:361956-361975 Msa0527740:CDS 55.0%
GGTTCAGAAACAACCACCGC+CGG - chr4_1:361978-361997 None:intergenic 55.0%
GGATCCGGCAACGAAGATGA+CGG - chr4_1:361999-362018 None:intergenic 55.0%
GTCATCTTCGTTGCCGGATC+CGG + chr4_1:361998-362017 Msa0527740:CDS 55.0%
! TGACGTGTAAGAAGCGCGTG+GGG + chr4_1:362075-362094 Msa0527740:CDS 55.0%
AGCGAATCCGGTGGTGAAAG+GGG + chr4_1:362211-362230 Msa0527740:CDS 55.0%
GGTGGTGAAAGGGGAGAAGT+TGG + chr4_1:362220-362239 Msa0527740:CDS 55.0%
TCTTCGTTGCCGGATCCGGT+TGG + chr4_1:362002-362021 Msa0527740:CDS 60.0%
! TAAGAAGCGCGTGGGGCTGA+CGG + chr4_1:362082-362101 Msa0527740:CDS 60.0%
ATGTGGGACCCACCGTTACC+CGG + chr4_1:362133-362152 Msa0527740:CDS 60.0%
! AACTCACACGCGTGCTGCTC+CGG - chr4_1:362155-362174 None:intergenic 60.0%
CATCGCAGCGTCTTCTCCGG+CGG + chr4_1:361959-361978 Msa0527740:CDS 65.0%
GTTGCCGGATCCGGTTGGAC+CGG + chr4_1:362007-362026 Msa0527740:CDS 65.0%
! AAGAAGCGCGTGGGGCTGAC+GGG + chr4_1:362083-362102 Msa0527740:CDS 65.0%
! GTGCTGCTCCGGGTAACGGT+GGG - chr4_1:362144-362163 None:intergenic 65.0%
! ACGCGTGCTGCTCCGGGTAA+CGG - chr4_1:362148-362167 None:intergenic 65.0%
! ACTCACACGCGTGCTGCTCC+GGG - chr4_1:362154-362173 None:intergenic 65.0%
CGCCGGAGAAGACGCTGCGA+TGG - chr4_1:361961-361980 None:intergenic 70.0%
CTCGCCGGTCCAACCGGATC+CGG - chr4_1:362014-362033 None:intergenic 70.0%
GCAACGCTCGCCGGTCCAAC+CGG - chr4_1:362020-362039 None:intergenic 70.0%
! GGTTGGACCGGCGAGCGTTG+CGG + chr4_1:362019-362038 Msa0527740:CDS 70.0%
TCCTCCACCGCAACGCTCGC+CGG - chr4_1:362029-362048 None:intergenic 70.0%
! AGAAGCGCGTGGGGCTGACG+GGG + chr4_1:362084-362103 Msa0527740:CDS 70.0%
! CGTGCTGCTCCGGGTAACGG+TGG - chr4_1:362145-362164 None:intergenic 70.0%
CGCAGCGTCTTCTCCGGCGG+TGG + chr4_1:361962-361981 Msa0527740:CDS 75.0%
! TGGACCGGCGAGCGTTGCGG+TGG + chr4_1:362022-362041 Msa0527740:CDS 75.0%
! ACCGGCGAGCGTTGCGGTGG+AGG + chr4_1:362025-362044 Msa0527740:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 361801 362253 361801 ID=Msa0527740;Name=Msa0527740
chr4_1 mRNA 361801 362253 361801 ID=Msa0527740-mRNA-1;Parent=Msa0527740;Name=Msa0527740-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|150
chr4_1 exon 361801 362253 361801 ID=Msa0527740-mRNA-1:exon:22;Parent=Msa0527740-mRNA-1
chr4_1 CDS 361801 362253 361801 ID=Msa0527740-mRNA-1:cds;Parent=Msa0527740-mRNA-1
Gene Sequence

>Msa0527740

ATGGCGGAAGAACACAGATTGCAAGCACCACGATTATGCGTCAACAATTGCGGTTTCTTCGGCAACGCCGCAACGGAGAATCTCTGTTCAAGATGCCATCGTGATTTACAGCTCAAAGAACAACAAGCTTCATCAGCCAAACTCGTTCTCAATCAATCCATCGCAGCGTCTTCTCCGGCGGTGGTTGTTTCTGAACCGTCATCTTCGTTGCCGGATCCGGTTGGACCGGCGAGCGTTGCGGTGGAGGAGAAACCGTTGCAACAGAATCGGTGTATGACGTGTAAGAAGCGCGTGGGGCTGACGGGGTTTAAGTGCAAGTGTGGGAGGATGTTATGTGGGACCCACCGTTACCCGGAGCAGCACGCGTGTGAGTTTGATTTTAAGGGGTTGGGGAAAGAGCAGATTGAGAAAGCGAATCCGGTGGTGAAAGGGGAGAAGTTGGAGAAGATCTGA

Protein sequence

>Msa0527740

MAEEHRLQAPRLCVNNCGFFGNAATENLCSRCHRDLQLKEQQASSAKLVLNQSIAASSPAVVVSEPSSSLPDPVGPASVAVEEKPLQQNRCMTCKKRVGLTGFKCKCGRMLCGTHRYPEQHACEFDFKGLGKEQIEKANPVVKGEKLEKI*