Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0227140 | XP_003597973.1 | 92.174 | 230 | 15 | 3 | 6 | 234 | 2 | 229 | 1.93e-148 | 423 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0227140 | sp|Q9T072|BH025_ARATH | 40.306 | 196 | 102 | 5 | 38 | 225 | 133 | 321 | 5.64e-34 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0227140 | G7IMB4 | 92.174 | 230 | 15 | 3 | 6 | 234 | 2 | 229 | 9.21e-149 | 423 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0227140 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0032040 | Msa0227140 | 0.816843 | 4.487871e-52 | -8.615850e-47 |
| Msa0040190 | Msa0227140 | 0.819553 | 1.093186e-52 | -8.615850e-47 |
| Msa0087880 | Msa0227140 | 0.814677 | 1.363751e-51 | -8.615850e-47 |
| Msa0123940 | Msa0227140 | 0.807156 | 5.799030e-50 | -8.615850e-47 |
| Msa0134550 | Msa0227140 | 0.828254 | 9.955172e-55 | -8.615850e-47 |
| Msa0189050 | Msa0227140 | 0.811653 | 6.286082e-51 | -8.615850e-47 |
| Msa0227140 | Msa0231310 | 0.806531 | 7.863622e-50 | -8.615850e-47 |
| Msa0227140 | Msa0253410 | 0.814928 | 1.199845e-51 | -8.615850e-47 |
| Msa0227140 | Msa0305430 | 0.855268 | 7.071714e-62 | -8.615850e-47 |
| Msa0227140 | Msa0309160 | 0.808572 | 2.899167e-50 | -8.615850e-47 |
| Msa0227140 | Msa0415460 | 0.807515 | 4.868444e-50 | -8.615850e-47 |
| Msa0227140 | Msa0475830 | 0.817339 | 3.471864e-52 | -8.615850e-47 |
| Msa0227140 | Msa0482580 | 0.803999 | 2.666433e-49 | -8.615850e-47 |
| Msa0227140 | Msa0498320 | 0.808955 | 2.401369e-50 | -8.615850e-47 |
| Msa0227140 | Msa0575250 | 0.803811 | 2.916830e-49 | -8.615850e-47 |
| Msa0227140 | Msa0598610 | 0.803433 | 3.494454e-49 | -8.615850e-47 |
| Msa0227140 | Msa0619060 | 0.820276 | 7.469974e-53 | -8.615850e-47 |
| Msa0227140 | Msa0619330 | 0.806185 | 9.299521e-50 | -8.615850e-47 |
| Msa0227140 | Msa0659130 | 0.801962 | 7.028178e-49 | -8.615850e-47 |
| Msa0227140 | Msa0705610 | 0.801221 | 9.975070e-49 | -8.615850e-47 |
| Msa0227140 | Msa0975910 | 0.806821 | 6.827716e-50 | -8.615850e-47 |
| Msa0227140 | Msa1096280 | 0.811859 | 5.671192e-51 | -8.615850e-47 |
| Msa0227140 | Msa1116010 | 0.813799 | 2.132233e-51 | -8.615850e-47 |
| Msa0227140 | Msa1157160 | 0.813575 | 2.388301e-51 | -8.615850e-47 |
| Msa0227140 | Msa1166520 | 0.801884 | 7.293583e-49 | -8.615850e-47 |
| Msa0227140 | Msa1208070 | 0.809079 | 2.259189e-50 | -8.615850e-47 |
| Msa0227140 | Msa1256280 | 0.809302 | 2.023839e-50 | -8.615850e-47 |
| Msa0227140 | Msa1357170 | 0.815271 | 1.006745e-51 | -8.615850e-47 |
| Msa0227140 | Msa1390410 | 0.808334 | 3.259579e-50 | -8.615850e-47 |
| Msa0227140 | Msa1415110 | 0.973276 | 3.720266e-136 | -8.615850e-47 |
| Msa0227140 | Msa1432470 | 0.806039 | 9.982317e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0227140 | MtrunA17_Chr2g0333611 | 92.342 | 222 | 14 | 3 | 14 | 234 | 1 | 220 | 1.36e-146 | 407 |
| Msa0227140 | MtrunA17_Chr2g0333621 | 46.798 | 203 | 93 | 3 | 37 | 231 | 118 | 313 | 1.07e-51 | 170 |
| Msa0227140 | MtrunA17_Chr8g0336681 | 46.231 | 199 | 94 | 3 | 45 | 233 | 144 | 339 | 1.08e-50 | 168 |
| Msa0227140 | MtrunA17_Chr4g0002801 | 47.692 | 195 | 86 | 3 | 47 | 230 | 142 | 331 | 3.64e-50 | 166 |
| Msa0227140 | MtrunA17_Chr4g0002781 | 47.396 | 192 | 88 | 3 | 47 | 230 | 164 | 350 | 5.18e-48 | 161 |
| Msa0227140 | MtrunA17_Chr2g0333641 | 45.128 | 195 | 95 | 3 | 37 | 226 | 117 | 304 | 1.48e-43 | 149 |
| Msa0227140 | MtrunA17_Chr5g0400661 | 43.316 | 187 | 85 | 5 | 56 | 225 | 148 | 330 | 1.07e-41 | 144 |
| Msa0227140 | MtrunA17_Chr5g0400621 | 42.553 | 188 | 94 | 4 | 50 | 230 | 150 | 330 | 7.32e-41 | 142 |
| Msa0227140 | MtrunA17_Chr2g0280161 | 43.258 | 178 | 90 | 4 | 54 | 225 | 174 | 346 | 3.57e-40 | 141 |
| Msa0227140 | MtrunA17_Chr5g0400671 | 41.304 | 184 | 101 | 4 | 48 | 229 | 145 | 323 | 4.34e-39 | 137 |
| Msa0227140 | MtrunA17_Chr4g0054581 | 41.667 | 204 | 101 | 7 | 38 | 231 | 181 | 376 | 6.23e-39 | 138 |
| Msa0227140 | MtrunA17_Chr7g0249811 | 45.745 | 188 | 92 | 4 | 47 | 233 | 154 | 332 | 2.58e-38 | 135 |
| Msa0227140 | MtrunA17_Chr2g0307131 | 36.364 | 198 | 123 | 3 | 38 | 234 | 161 | 356 | 5.91e-38 | 135 |
| Msa0227140 | MtrunA17_Chr4g0049331 | 40.449 | 178 | 90 | 4 | 56 | 225 | 134 | 303 | 1.52e-37 | 133 |
| Msa0227140 | MtrunA17_Chr2g0333541 | 43.541 | 209 | 93 | 6 | 31 | 225 | 67 | 264 | 6.07e-37 | 130 |
| Msa0227140 | MtrunA17_Chr6g0468491 | 39.109 | 202 | 114 | 4 | 34 | 231 | 115 | 311 | 2.86e-36 | 130 |
| Msa0227140 | MtrunA17_Chr4g0054561 | 39.785 | 186 | 102 | 5 | 50 | 231 | 148 | 327 | 1.46e-35 | 128 |
| Msa0227140 | MtrunA17_Chr4g0033391 | 36.082 | 194 | 119 | 2 | 38 | 231 | 130 | 318 | 4.15e-35 | 127 |
| Msa0227140 | MtrunA17_Chr2g0307141 | 37.288 | 177 | 109 | 2 | 56 | 231 | 182 | 357 | 8.66e-35 | 127 |
| Msa0227140 | MtrunA17_Chr4g0033291 | 39.894 | 188 | 108 | 2 | 44 | 231 | 144 | 326 | 2.46e-34 | 125 |
| Msa0227140 | MtrunA17_Chr4g0054621 | 34.419 | 215 | 116 | 4 | 35 | 231 | 132 | 339 | 5.34e-34 | 124 |
| Msa0227140 | MtrunA17_Chr4g0054551 | 37.968 | 187 | 104 | 5 | 50 | 231 | 148 | 327 | 1.24e-33 | 123 |
| Msa0227140 | MtrunA17_Chr4g0033281 | 36.181 | 199 | 99 | 4 | 44 | 231 | 142 | 323 | 8.79e-33 | 121 |
| Msa0227140 | MtrunA17_Chr7g0225901 | 36.967 | 211 | 106 | 8 | 16 | 209 | 40 | 240 | 1.56e-32 | 119 |
| Msa0227140 | MtrunA17_Chr4g0054571 | 37.745 | 204 | 104 | 4 | 43 | 231 | 136 | 331 | 2.62e-29 | 112 |
| Msa0227140 | MtrunA17_Chr6g0468461 | 34.021 | 194 | 101 | 4 | 16 | 209 | 145 | 311 | 4.88e-28 | 108 |
| Msa0227140 | MtrunA17_Chr5g0400641 | 36.810 | 163 | 89 | 4 | 60 | 215 | 1 | 156 | 9.11e-27 | 101 |
| Msa0227140 | MtrunA17_Chr4g0002831 | 37.725 | 167 | 92 | 4 | 60 | 225 | 45 | 200 | 5.70e-26 | 100 |
| Msa0227140 | MtrunA17_Chr7g0233441 | 30.667 | 225 | 123 | 5 | 1 | 225 | 1 | 192 | 1.56e-25 | 99.0 |
| Msa0227140 | MtrunA17_Chr4g0002851 | 30.131 | 229 | 119 | 7 | 1 | 225 | 1 | 192 | 3.20e-25 | 98.2 |
| Msa0227140 | MtrunA17_Chr8g0336671 | 52.308 | 65 | 29 | 1 | 23 | 87 | 3 | 65 | 3.95e-14 | 65.5 |
| Msa0227140 | MtrunA17_Chr1g0185621 | 28.777 | 139 | 91 | 2 | 58 | 188 | 476 | 614 | 3.79e-13 | 68.2 |
| Msa0227140 | MtrunA17_Chr7g0275821 | 25.294 | 170 | 118 | 4 | 58 | 227 | 253 | 413 | 4.08e-13 | 67.8 |
| Msa0227140 | MtrunA17_Chr2g0298751 | 22.581 | 217 | 142 | 4 | 30 | 233 | 34 | 237 | 1.01e-11 | 62.8 |
| Msa0227140 | MtrunA17_Chr5g0411341 | 29.787 | 188 | 107 | 8 | 58 | 228 | 494 | 673 | 2.57e-11 | 62.8 |
| Msa0227140 | MtrunA17_Chr8g0386921 | 47.273 | 55 | 29 | 0 | 58 | 112 | 407 | 461 | 9.44e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0227140 | AT4G37850.2 | 40.306 | 196 | 102 | 5 | 38 | 225 | 133 | 321 | 5.74e-35 | 127 |
| Msa0227140 | AT4G37850.1 | 40.306 | 196 | 102 | 5 | 38 | 225 | 133 | 321 | 5.74e-35 | 127 |
| Msa0227140 | AT2G22760.1 | 37.634 | 186 | 99 | 5 | 40 | 217 | 106 | 282 | 1.30e-34 | 125 |
| Msa0227140 | AT2G22760.2 | 38.202 | 178 | 93 | 5 | 40 | 209 | 106 | 274 | 3.28e-32 | 119 |
| Msa0227140 | AT2G22770.1 | 38.542 | 192 | 91 | 5 | 55 | 225 | 130 | 315 | 1.98e-31 | 117 |
| Msa0227140 | AT2G22750.2 | 37.688 | 199 | 96 | 5 | 49 | 231 | 118 | 304 | 2.07e-29 | 112 |
| Msa0227140 | AT2G22750.5 | 37.688 | 199 | 96 | 5 | 49 | 231 | 142 | 328 | 3.13e-29 | 112 |
| Msa0227140 | AT2G22750.3 | 37.688 | 199 | 96 | 5 | 49 | 231 | 146 | 332 | 3.79e-29 | 112 |
| Msa0227140 | AT2G22750.1 | 37.688 | 199 | 95 | 6 | 49 | 231 | 118 | 303 | 6.01e-28 | 108 |
| Msa0227140 | AT2G22750.4 | 37.688 | 199 | 95 | 6 | 49 | 231 | 146 | 331 | 9.61e-28 | 108 |
| Msa0227140 | AT4G09820.1 | 32.394 | 142 | 76 | 3 | 43 | 184 | 349 | 470 | 1.55e-15 | 75.5 |
| Msa0227140 | AT5G57150.3 | 25.822 | 213 | 125 | 7 | 1 | 197 | 9 | 204 | 2.13e-13 | 67.4 |
| Msa0227140 | AT5G57150.2 | 25.822 | 213 | 125 | 7 | 1 | 197 | 9 | 204 | 2.38e-13 | 67.4 |
| Msa0227140 | AT4G29930.6 | 24.554 | 224 | 137 | 7 | 3 | 218 | 10 | 209 | 3.02e-13 | 67.0 |
| Msa0227140 | AT5G57150.4 | 25.822 | 213 | 125 | 7 | 1 | 197 | 9 | 204 | 4.40e-13 | 67.4 |
| Msa0227140 | AT5G57150.1 | 25.217 | 230 | 140 | 7 | 1 | 215 | 9 | 221 | 5.43e-13 | 66.6 |
| Msa0227140 | AT4G29930.2 | 23.394 | 218 | 144 | 5 | 3 | 218 | 10 | 206 | 6.20e-13 | 66.2 |
| Msa0227140 | AT5G57150.5 | 25.943 | 212 | 125 | 7 | 1 | 197 | 9 | 203 | 1.51e-12 | 65.1 |
| Msa0227140 | AT5G46760.1 | 25.824 | 182 | 104 | 3 | 58 | 213 | 416 | 592 | 1.63e-12 | 66.6 |
| Msa0227140 | AT1G63650.3 | 29.412 | 153 | 86 | 4 | 54 | 186 | 402 | 552 | 3.16e-12 | 65.9 |
| Msa0227140 | AT1G63650.1 | 29.412 | 153 | 86 | 4 | 54 | 186 | 402 | 552 | 3.16e-12 | 65.9 |
| Msa0227140 | AT1G63650.2 | 29.412 | 153 | 86 | 4 | 54 | 186 | 402 | 552 | 3.16e-12 | 65.9 |
| Msa0227140 | AT5G41315.2 | 26.752 | 157 | 90 | 3 | 54 | 186 | 446 | 601 | 3.53e-12 | 65.9 |
| Msa0227140 | AT5G41315.3 | 26.752 | 157 | 90 | 3 | 54 | 186 | 443 | 598 | 3.62e-12 | 65.9 |
| Msa0227140 | AT5G41315.1 | 26.752 | 157 | 90 | 3 | 54 | 186 | 438 | 593 | 3.75e-12 | 65.9 |
| Msa0227140 | AT4G29930.5 | 23.469 | 196 | 128 | 4 | 3 | 196 | 10 | 185 | 4.69e-12 | 63.9 |
| Msa0227140 | AT1G12860.1 | 24.713 | 174 | 123 | 3 | 58 | 224 | 268 | 440 | 6.83e-12 | 64.7 |
| Msa0227140 | AT1G01260.1 | 27.586 | 174 | 100 | 5 | 54 | 227 | 430 | 577 | 7.96e-12 | 64.7 |
| Msa0227140 | AT1G01260.2 | 27.586 | 174 | 100 | 5 | 54 | 227 | 430 | 577 | 7.96e-12 | 64.7 |
| Msa0227140 | AT1G01260.3 | 27.586 | 174 | 100 | 5 | 54 | 227 | 430 | 577 | 7.96e-12 | 64.7 |
| Msa0227140 | AT2G46510.1 | 39.394 | 66 | 40 | 0 | 58 | 123 | 396 | 461 | 8.62e-12 | 64.7 |
| Msa0227140 | AT4G17880.1 | 28.415 | 183 | 105 | 7 | 58 | 228 | 417 | 585 | 3.46e-11 | 62.8 |
| Msa0227140 | AT4G29930.1 | 23.013 | 239 | 148 | 8 | 3 | 218 | 10 | 235 | 3.57e-11 | 61.6 |
| Msa0227140 | AT1G12860.2 | 26.144 | 153 | 105 | 3 | 58 | 203 | 268 | 419 | 5.40e-11 | 62.0 |
| Msa0227140 | AT1G32640.1 | 25.000 | 140 | 98 | 2 | 58 | 193 | 453 | 589 | 6.41e-11 | 62.0 |
Find 66 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCCACGTAATGATAATT+TGG | 0.206550 | 2_1:-77794438 | Msa0227140:CDS |
| ATGCCAATGGCCTCCACTTC+AGG | 0.214258 | 2_1:+77794395 | None:intergenic |
| CCATGTGTAGCAGCATTATT+AGG | 0.265823 | 2_1:+77796155 | None:intergenic |
| GGAGAATTGGTTCTCTAATA+TGG | 0.295421 | 2_1:-77796372 | Msa0227140:intron |
| CAGAAGCATCCAACAATAAT+AGG | 0.297499 | 2_1:-77796197 | Msa0227140:CDS |
| TTCTTAAGAGTTGGAGTTGT+AGG | 0.299359 | 2_1:-77794005 | Msa0227140:three_prime_UTR |
| ACATAATGTCAGAGAGAAAA+AGG | 0.300304 | 2_1:-77796091 | Msa0227140:CDS |
| TTAGGTATAGATAAAGTAAA+AGG | 0.327176 | 2_1:+77796410 | None:intergenic |
| GAACAATGTCCTATTATTGT+TGG | 0.353321 | 2_1:+77796188 | None:intergenic |
| TGTATTTATCGGGTGATCTA+TGG | 0.368338 | 2_1:+77796441 | None:intergenic |
| ATAGACAAGGTTTCTGTACT+TGG | 0.374394 | 2_1:-77794578 | Msa0227140:CDS |
| ACCAGCAGTGTGCTTCCATT+TGG | 0.381044 | 2_1:-77794266 | Msa0227140:CDS |
| TGGAGAATTGGTGGGAGAAT+TGG | 0.381146 | 2_1:-77796385 | Msa0227140:CDS |
| GTTCAAACTATTGTCAATGC+TGG | 0.402081 | 2_1:-77794312 | Msa0227140:CDS |
| CTATACCTAACATGGAGAAT+TGG | 0.414546 | 2_1:-77796397 | Msa0227140:exon |
| AGAGAGATTCATAGCACTTT+CGG | 0.417342 | 2_1:-77796054 | Msa0227140:CDS |
| GGCAGATGGGTGAGGAATAC+AGG | 0.417383 | 2_1:-77794127 | Msa0227140:intron |
| ATTGATGACAAAGTTGAAAC+AGG | 0.417457 | 2_1:-77794090 | Msa0227140:CDS |
| TCCCACCAATTCTCCATGTT+AGG | 0.422680 | 2_1:+77796392 | None:intergenic |
| TCTTAAGAGTTGGAGTTGTA+GGG | 0.440373 | 2_1:-77794004 | Msa0227140:three_prime_UTR |
| AAGTTATAGTTCTTAAGAGT+TGG | 0.441351 | 2_1:-77794014 | Msa0227140:three_prime_UTR |
| TTCAGGGAGGAGCACATGAT+TGG | 0.447101 | 2_1:+77794412 | None:intergenic |
| TGCCAATGGCCTCCACTTCA+GGG | 0.452486 | 2_1:+77794396 | None:intergenic |
| AAACATTACCTTAGCAATGA+TGG | 0.455229 | 2_1:+77794223 | None:intergenic |
| AAGGAGAGGGATCGTGAAGC+TGG | 0.456447 | 2_1:+77796505 | None:intergenic |
| CTCCCTGAAGTGGAGGCCAT+TGG | 0.457623 | 2_1:-77794398 | Msa0227140:CDS |
| AGGAGGGTATCTGTATTTAT+CGG | 0.461686 | 2_1:+77796430 | None:intergenic |
| AATAATTGTGTGTGAAGGAG+AGG | 0.462148 | 2_1:+77796491 | None:intergenic |
| CTACACATGGAAAGAATAAG+AGG | 0.462322 | 2_1:-77796142 | Msa0227140:CDS |
| TGTTGGATGCTTCTGATTGA+AGG | 0.471742 | 2_1:+77796205 | None:intergenic |
| CTTCTCTGATTCTATGCCAA+TGG | 0.479471 | 2_1:+77794382 | None:intergenic |
| TCCAAATGGAAGCACACTGC+TGG | 0.484948 | 2_1:+77794265 | None:intergenic |
| TTAATATCTATAGGATATGA+AGG | 0.498841 | 2_1:-77796240 | Msa0227140:intron |
| TAAAGAACGCATATCAATGC+TGG | 0.499426 | 2_1:-77794528 | Msa0227140:CDS |
| GGTATAGATAAAGTAAAAGG+AGG | 0.507865 | 2_1:+77796413 | None:intergenic |
| GGAGGGTATCTGTATTTATC+GGG | 0.511314 | 2_1:+77796431 | None:intergenic |
| AGCAATGATGGTAATGTTGA+GGG | 0.515073 | 2_1:+77794235 | None:intergenic |
| CCTAATAATGCTGCTACACA+TGG | 0.515165 | 2_1:-77796155 | Msa0227140:CDS |
| TACCTAACATGGAGAATTGG+TGG | 0.525198 | 2_1:-77796394 | Msa0227140:exon |
| TATGATACCCGGCTTGAAGA+AGG | 0.532421 | 2_1:-77796030 | Msa0227140:intron |
| TACTTTATCTATACCTAACA+TGG | 0.533893 | 2_1:-77796405 | Msa0227140:exon |
| AAATGGAAGCACACTGCTGG+TGG | 0.542174 | 2_1:+77794268 | None:intergenic |
| GCACTTTCGGCTATGATACC+CGG | 0.547609 | 2_1:-77796041 | Msa0227140:CDS |
| TCATGTGCTCCTCCCTGAAG+TGG | 0.548063 | 2_1:-77794408 | Msa0227140:CDS |
| GGAACTGACCTTCTTCAAGC+CGG | 0.556400 | 2_1:+77796022 | None:intergenic |
| TAGCAATGATGGTAATGTTG+AGG | 0.557198 | 2_1:+77794234 | None:intergenic |
| TAAGAGGGTGAGAAGCTCAT+GGG | 0.563045 | 2_1:-77796126 | Msa0227140:CDS |
| CAACATTACCATCATTGCTA+AGG | 0.570598 | 2_1:-77794231 | Msa0227140:intron |
| GTATAGATAAAGTAAAAGGA+GGG | 0.576956 | 2_1:+77796414 | None:intergenic |
| ATAAGAGGGTGAGAAGCTCA+TGG | 0.579940 | 2_1:-77796127 | Msa0227140:CDS |
| GAACTGACCTTCTTCAAGCC+GGG | 0.582458 | 2_1:+77796023 | None:intergenic |
| GGGTATGAATGTGAACAGAA+AGG | 0.582478 | 2_1:-77793984 | Msa0227140:three_prime_UTR |
| ATAATTGTGTGTGAAGGAGA+GGG | 0.583451 | 2_1:+77796492 | None:intergenic |
| ATCAACTGCGAGAAACATGA+AGG | 0.584639 | 2_1:-77794341 | Msa0227140:CDS |
| CACTGCTGGTGGAAACATAG+AGG | 0.588212 | 2_1:+77794279 | None:intergenic |
| ACCTAACATGGAGAATTGGT+GGG | 0.590580 | 2_1:-77796393 | Msa0227140:exon |
| TGTGCTCCTCCCTGAAGTGG+AGG | 0.603078 | 2_1:-77794405 | Msa0227140:CDS |
| AGAAGCTCATGGGAGATACA+AGG | 0.603853 | 2_1:-77796116 | Msa0227140:CDS |
| ACTTGTTAGATACACTAGCA+TGG | 0.633985 | 2_1:+77794033 | None:intergenic |
| GAATCCAAATTATCATTACG+TGG | 0.646914 | 2_1:+77794434 | None:intergenic |
| CATTGGCATAGAATCAGAGA+AGG | 0.647651 | 2_1:-77794381 | Msa0227140:CDS |
| TACACATGGAAAGAATAAGA+GGG | 0.651853 | 2_1:-77796141 | Msa0227140:CDS |
| TAATATCTATAGGATATGAA+GGG | 0.651855 | 2_1:-77796239 | Msa0227140:intron |
| TGGAGCAACAATGTTATGAG+AGG | 0.657981 | 2_1:-77794508 | Msa0227140:CDS |
| TTTACTTGTATAGATAGACA+AGG | 0.695353 | 2_1:-77794591 | Msa0227140:intron |
| CAATGGCCTCCACTTCAGGG+AGG | 0.758935 | 2_1:+77794399 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAATAATAAATTAACAC+AGG | - | chr2_1:77795495-77795514 | Msa0227140:intron | 10.0% |
| !!! | AAATTTTTTAATAGAGATTA+TGG | + | chr2_1:77796011-77796030 | None:intergenic | 10.0% |
| !!! | ATTAACTTTTTATAATGGAT+AGG | - | chr2_1:77794574-77794593 | Msa0227140:CDS | 15.0% |
| !! | GAAAAAAATTAAAGTTGTAT+TGG | - | chr2_1:77794801-77794820 | Msa0227140:intron | 15.0% |
| !!! | CTTTTTTTGACAATATTTAA+TGG | - | chr2_1:77794914-77794933 | Msa0227140:intron | 15.0% |
| !! | TACAATATAATTGAATTACT+TGG | + | chr2_1:77794970-77794989 | None:intergenic | 15.0% |
| !!! | ATAATTAAGCTTGTTAATTT+TGG | + | chr2_1:77795361-77795380 | None:intergenic | 15.0% |
| !! | TTGATAAAATGTTAATTACA+TGG | - | chr2_1:77796315-77796334 | Msa0227140:intron | 15.0% |
| !! | AAAAAAATAATTGTGTGTGA+AGG | + | chr2_1:77793991-77794010 | None:intergenic | 20.0% |
| !! | TTAGGTATAGATAAAGTAAA+AGG | + | chr2_1:77794067-77794086 | None:intergenic | 20.0% |
| !! | TATAGATATTAAACACAACA+AGG | + | chr2_1:77794226-77794245 | None:intergenic | 20.0% |
| !! | TTGTTGTGTTTAATATCTAT+AGG | - | chr2_1:77794225-77794244 | Msa0227140:intron | 20.0% |
| !! | TTAATATCTATAGGATATGA+AGG | - | chr2_1:77794234-77794253 | Msa0227140:CDS | 20.0% |
| !! | TAATATCTATAGGATATGAA+GGG | - | chr2_1:77794235-77794254 | Msa0227140:CDS | 20.0% |
| !! | AAACATGGAAATACTAAATA+TGG | - | chr2_1:77794530-77794549 | Msa0227140:CDS | 20.0% |
| !! | TTGTAAGATGTTTAAATAGT+GGG | + | chr2_1:77795013-77795032 | None:intergenic | 20.0% |
| !! | TTTGTAAGATGTTTAAATAG+TGG | + | chr2_1:77795014-77795033 | None:intergenic | 20.0% |
| !! | ATTAATTTAGTTGAGATACA+TGG | + | chr2_1:77795207-77795226 | None:intergenic | 20.0% |
| !!! | ATTTTTAGTATAGTCATTAG+AGG | - | chr2_1:77795290-77795309 | Msa0227140:intron | 20.0% |
| !! | GACATAAAATAAATTAACAC+AGG | - | chr2_1:77795425-77795444 | Msa0227140:intron | 20.0% |
| !! | AAATAAAGAGTGAGAATAAA+AGG | - | chr2_1:77795693-77795712 | Msa0227140:intron | 20.0% |
| !! | AATAAAGAGTGAGAATAAAA+GGG | - | chr2_1:77795694-77795713 | Msa0227140:intron | 20.0% |
| !!! | TTCAAGTATTTTTGTTGATA+AGG | - | chr2_1:77795745-77795764 | Msa0227140:intron | 20.0% |
| !!! | TTTTTACACTCTTAAGTTTT+AGG | - | chr2_1:77795813-77795832 | Msa0227140:intron | 20.0% |
| !!! | TTAATTACATGGATTTTATG+TGG | - | chr2_1:77796326-77796345 | Msa0227140:intron | 20.0% |
| ! | GTATAGATAAAGTAAAAGGA+GGG | + | chr2_1:77794063-77794082 | None:intergenic | 25.0% |
| ! | TACTTTATCTATACCTAACA+TGG | - | chr2_1:77794069-77794088 | Msa0227140:CDS | 25.0% |
| !!! | GTTTTTTGTAGATCAAAACA+TGG | - | chr2_1:77794515-77794534 | Msa0227140:CDS | 25.0% |
| !! | CTAAATATGGATTAAACATG+AGG | - | chr2_1:77794543-77794562 | Msa0227140:CDS | 25.0% |
| !!! | TGTCTTGTTCTTTTAAAGTA+AGG | + | chr2_1:77794712-77794731 | None:intergenic | 25.0% |
| !! | AAAAGTTGGAGTATTTATTG+AGG | - | chr2_1:77794766-77794785 | Msa0227140:intron | 25.0% |
| !! | AAAGTTGGAGTATTTATTGA+GGG | - | chr2_1:77794767-77794786 | Msa0227140:intron | 25.0% |
| !!! | AAGTGACAATCATTTTGAAA+CGG | - | chr2_1:77794866-77794885 | Msa0227140:intron | 25.0% |
| ! | TTGTATTTAGTAGTGATACA+TGG | - | chr2_1:77794985-77795004 | Msa0227140:intron | 25.0% |
| ! | TGTAAGATGTTTAAATAGTG+GGG | + | chr2_1:77795012-77795031 | None:intergenic | 25.0% |
| ! | TACAAGATTAATTCAGTTCA+TGG | - | chr2_1:77795085-77795104 | Msa0227140:intron | 25.0% |
| !! | TTGGCTTATCAATGTATTTT+GGG | + | chr2_1:77795251-77795270 | None:intergenic | 25.0% |
| !!! | TTTGGCTTATCAATGTATTT+TGG | + | chr2_1:77795252-77795271 | None:intergenic | 25.0% |
| ! | AATACATTGATAAGCCAAAA+AGG | - | chr2_1:77795253-77795272 | Msa0227140:intron | 25.0% |
| !! | AGATTTTCTTTGTACCTTTT+TGG | + | chr2_1:77795270-77795289 | None:intergenic | 25.0% |
| ! | TTTACTTGTATAGATAGACA+AGG | - | chr2_1:77795883-77795902 | Msa0227140:intron | 25.0% |
| ! | AAGTTATAGTTCTTAAGAGT+TGG | - | chr2_1:77796460-77796479 | Msa0227140:five_prime_UTR | 25.0% |
| GGTATAGATAAAGTAAAAGG+AGG | + | chr2_1:77794064-77794083 | None:intergenic | 30.0% | |
| GAACAATGTCCTATTATTGT+TGG | + | chr2_1:77794289-77794308 | None:intergenic | 30.0% | |
| TACACATGGAAAGAATAAGA+GGG | - | chr2_1:77794333-77794352 | Msa0227140:CDS | 30.0% | |
| ACATAATGTCAGAGAGAAAA+AGG | - | chr2_1:77794383-77794402 | Msa0227140:CDS | 30.0% | |
| GTATATATTGAAGAAGAAGC+AGG | + | chr2_1:77794476-77794495 | None:intergenic | 30.0% | |
| GTATTTATTGAGGGTAAAGT+TGG | - | chr2_1:77794776-77794795 | Msa0227140:intron | 30.0% | |
| ! | TAAGTTTGAGTTCTTATGCT+TGG | + | chr2_1:77795335-77795354 | None:intergenic | 30.0% |
| TTTGTTACTCACATTTACAG+AGG | + | chr2_1:77795401-77795420 | None:intergenic | 30.0% | |
| GTAAATGTGAGTAACAAAGA+AGG | - | chr2_1:77795403-77795422 | Msa0227140:intron | 30.0% | |
| ATTATGACATGTGACTTATG+AGG | - | chr2_1:77795782-77795801 | Msa0227140:intron | 30.0% | |
| TCATCCACGTAATGATAATT+TGG | - | chr2_1:77796036-77796055 | Msa0227140:CDS | 30.0% | |
| GAATCCAAATTATCATTACG+TGG | + | chr2_1:77796043-77796062 | None:intergenic | 30.0% | |
| !!! | TTGAGGGTATTTTTTCCAAA+TGG | + | chr2_1:77796226-77796245 | None:intergenic | 30.0% |
| AAACATTACCTTAGCAATGA+TGG | + | chr2_1:77796254-77796273 | None:intergenic | 30.0% | |
| ! | ATTGATGACAAAGTTGAAAC+AGG | - | chr2_1:77796384-77796403 | Msa0227140:CDS | 30.0% |
| ATAATTGTGTGTGAAGGAGA+GGG | + | chr2_1:77793985-77794004 | None:intergenic | 35.0% | |
| AATAATTGTGTGTGAAGGAG+AGG | + | chr2_1:77793986-77794005 | None:intergenic | 35.0% | |
| TGTATTTATCGGGTGATCTA+TGG | + | chr2_1:77794036-77794055 | None:intergenic | 35.0% | |
| !! | AGGAGGGTATCTGTATTTAT+CGG | + | chr2_1:77794047-77794066 | None:intergenic | 35.0% |
| CTATACCTAACATGGAGAAT+TGG | - | chr2_1:77794077-77794096 | Msa0227140:CDS | 35.0% | |
| ! | GGAGAATTGGTTCTCTAATA+TGG | - | chr2_1:77794102-77794121 | Msa0227140:CDS | 35.0% |
| !! | CAGAAGCATCCAACAATAAT+AGG | - | chr2_1:77794277-77794296 | Msa0227140:CDS | 35.0% |
| CTACACATGGAAAGAATAAG+AGG | - | chr2_1:77794332-77794351 | Msa0227140:CDS | 35.0% | |
| GAGAAAAAGGAGACAAGAAA+TGG | - | chr2_1:77794396-77794415 | Msa0227140:CDS | 35.0% | |
| AGAGAGATTCATAGCACTTT+CGG | - | chr2_1:77794420-77794439 | Msa0227140:CDS | 35.0% | |
| ! | AAAGCGACACTCATTTTGAA+AGG | + | chr2_1:77794621-77794640 | None:intergenic | 35.0% |
| ATTGTGTGAGAGAGAAAAGT+TGG | - | chr2_1:77794752-77794771 | Msa0227140:intron | 35.0% | |
| !!! | GACAATCATTTTGAAACGGA+TGG | - | chr2_1:77794870-77794889 | Msa0227140:intron | 35.0% |
| TCAGTTCATGGCAAAAATTG+TGG | - | chr2_1:77795097-77795116 | Msa0227140:intron | 35.0% | |
| ATCAATTTCGAGATGAAGCA+AGG | - | chr2_1:77795225-77795244 | Msa0227140:intron | 35.0% | |
| TTAACACAGGTGATTTCACA+TGG | - | chr2_1:77795438-77795457 | Msa0227140:intron | 35.0% | |
| ATAGACAAGGTTTCTGTACT+TGG | - | chr2_1:77795896-77795915 | Msa0227140:intron | 35.0% | |
| TAAAGAACGCATATCAATGC+TGG | - | chr2_1:77795946-77795965 | Msa0227140:intron | 35.0% | |
| GTTCAAACTATTGTCAATGC+TGG | - | chr2_1:77796162-77796181 | Msa0227140:CDS | 35.0% | |
| ! | AGCAATGATGGTAATGTTGA+GGG | + | chr2_1:77796242-77796261 | None:intergenic | 35.0% |
| ! | TAGCAATGATGGTAATGTTG+AGG | + | chr2_1:77796243-77796262 | None:intergenic | 35.0% |
| CAACATTACCATCATTGCTA+AGG | - | chr2_1:77796243-77796262 | Msa0227140:intron | 35.0% | |
| ! | ATGGATTTTATGTGGCAGAT+GGG | - | chr2_1:77796334-77796353 | Msa0227140:intron | 35.0% |
| ! | ACTTGTTAGATACACTAGCA+TGG | + | chr2_1:77796444-77796463 | None:intergenic | 35.0% |
| TTCTTAAGAGTTGGAGTTGT+AGG | - | chr2_1:77796469-77796488 | Msa0227140:five_prime_UTR | 35.0% | |
| TCTTAAGAGTTGGAGTTGTA+GGG | - | chr2_1:77796470-77796489 | Msa0227140:five_prime_UTR | 35.0% | |
| !! | GGAGGGTATCTGTATTTATC+GGG | + | chr2_1:77794046-77794065 | None:intergenic | 40.0% |
| TACCTAACATGGAGAATTGG+TGG | - | chr2_1:77794080-77794099 | Msa0227140:CDS | 40.0% | |
| ACCTAACATGGAGAATTGGT+GGG | - | chr2_1:77794081-77794100 | Msa0227140:CDS | 40.0% | |
| TGTTGGATGCTTCTGATTGA+AGG | + | chr2_1:77794272-77794291 | None:intergenic | 40.0% | |
| CCATGTGTAGCAGCATTATT+AGG | + | chr2_1:77794322-77794341 | None:intergenic | 40.0% | |
| CCTAATAATGCTGCTACACA+TGG | - | chr2_1:77794319-77794338 | Msa0227140:CDS | 40.0% | |
| !! | CGACACTCATTTTGAAAGGA+TGG | + | chr2_1:77794617-77794636 | None:intergenic | 40.0% |
| TTCCTTAATCCGTGTGCAAT+TGG | + | chr2_1:77794646-77794665 | None:intergenic | 40.0% | |
| ! | ATTGCACACGGATTAAGGAA+TGG | - | chr2_1:77794646-77794665 | Msa0227140:intron | 40.0% |
| !! | CGGATGGAGTACTATTTTAG+TGG | - | chr2_1:77794886-77794905 | Msa0227140:intron | 40.0% |
| GTTACTCACATTTACAGAGG+AGG | + | chr2_1:77795398-77795417 | None:intergenic | 40.0% | |
| TGGAGCAACAATGTTATGAG+AGG | - | chr2_1:77795966-77795985 | Msa0227140:intron | 40.0% | |
| CTTCTCTGATTCTATGCCAA+TGG | + | chr2_1:77796095-77796114 | None:intergenic | 40.0% | |
| CATTGGCATAGAATCAGAGA+AGG | - | chr2_1:77796093-77796112 | Msa0227140:CDS | 40.0% | |
| ATCAACTGCGAGAAACATGA+AGG | - | chr2_1:77796133-77796152 | Msa0227140:CDS | 40.0% | |
| ! | CATGGATTTTATGTGGCAGA+TGG | - | chr2_1:77796333-77796352 | Msa0227140:intron | 40.0% |
| GGGTATGAATGTGAACAGAA+AGG | - | chr2_1:77796490-77796509 | Msa0227140:five_prime_UTR | 40.0% | |
| TCCCACCAATTCTCCATGTT+AGG | + | chr2_1:77794085-77794104 | None:intergenic | 45.0% | |
| TGGAGAATTGGTGGGAGAAT+TGG | - | chr2_1:77794089-77794108 | Msa0227140:CDS | 45.0% | |
| ATAAGAGGGTGAGAAGCTCA+TGG | - | chr2_1:77794347-77794366 | Msa0227140:CDS | 45.0% | |
| TAAGAGGGTGAGAAGCTCAT+GGG | - | chr2_1:77794348-77794367 | Msa0227140:CDS | 45.0% | |
| AGAAGCTCATGGGAGATACA+AGG | - | chr2_1:77794358-77794377 | Msa0227140:CDS | 45.0% | |
| ! | TATGATACCCGGCTTGAAGA+AGG | - | chr2_1:77794444-77794463 | Msa0227140:CDS | 45.0% |
| TCGCTTTAGCCAATTGCACA+CGG | - | chr2_1:77794634-77794653 | Msa0227140:intron | 45.0% | |
| ! | AGCCAATTGCACACGGATTA+AGG | - | chr2_1:77794641-77794660 | Msa0227140:intron | 45.0% |
| TTCTTATGCTTGGAGCTTGG+TGG | + | chr2_1:77795325-77795344 | None:intergenic | 45.0% | |
| GAGTTCTTATGCTTGGAGCT+TGG | + | chr2_1:77795328-77795347 | None:intergenic | 45.0% | |
| ! | TTTTATGTGGCAGATGGGTG+AGG | - | chr2_1:77796339-77796358 | Msa0227140:intron | 45.0% |
| !!! | ATGAAATTATTTTTATAAAA+TGG | + | chr2_1:77794143-77794162 | None:intergenic | 5.0% |
| !!! | ATTATATTAACTTTTTATAA+TGG | - | chr2_1:77794569-77794588 | Msa0227140:CDS | 5.0% |
| GCACTTTCGGCTATGATACC+CGG | - | chr2_1:77794433-77794452 | Msa0227140:CDS | 50.0% | |
| GAACTGACCTTCTTCAAGCC+GGG | + | chr2_1:77794454-77794473 | None:intergenic | 50.0% | |
| GGAACTGACCTTCTTCAAGC+CGG | + | chr2_1:77794455-77794474 | None:intergenic | 50.0% | |
| ! | TTCAGGGAGGAGCACATGAT+TGG | + | chr2_1:77796065-77796084 | None:intergenic | 50.0% |
| CACTGCTGGTGGAAACATAG+AGG | + | chr2_1:77796198-77796217 | None:intergenic | 50.0% | |
| ! | AAATGGAAGCACACTGCTGG+TGG | + | chr2_1:77796209-77796228 | None:intergenic | 50.0% |
| ACCAGCAGTGTGCTTCCATT+TGG | - | chr2_1:77796208-77796227 | Msa0227140:CDS | 50.0% | |
| ! | TCCAAATGGAAGCACACTGC+TGG | + | chr2_1:77796212-77796231 | None:intergenic | 50.0% |
| AAGGAGAGGGATCGTGAAGC+TGG | + | chr2_1:77793972-77793991 | None:intergenic | 55.0% | |
| TCATGTGCTCCTCCCTGAAG+TGG | - | chr2_1:77796066-77796085 | Msa0227140:CDS | 55.0% | |
| TGCCAATGGCCTCCACTTCA+GGG | + | chr2_1:77796081-77796100 | None:intergenic | 55.0% | |
| ATGCCAATGGCCTCCACTTC+AGG | + | chr2_1:77796082-77796101 | None:intergenic | 55.0% | |
| GGCAGATGGGTGAGGAATAC+AGG | - | chr2_1:77796347-77796366 | Msa0227140:intron | 55.0% | |
| TGTGCTCCTCCCTGAAGTGG+AGG | - | chr2_1:77796069-77796088 | Msa0227140:CDS | 60.0% | |
| CAATGGCCTCCACTTCAGGG+AGG | + | chr2_1:77796078-77796097 | None:intergenic | 60.0% | |
| CTCCCTGAAGTGGAGGCCAT+TGG | - | chr2_1:77796076-77796095 | Msa0227140:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 77793969 | 77796527 | 77793969 | ID=Msa0227140;Name=Msa0227140 |
| chr2_1 | mRNA | 77793969 | 77796527 | 77793969 | ID=Msa0227140-mRNA-1;Parent=Msa0227140;Name=Msa0227140-mRNA-1;_AED=0.21;_eAED=0.21;_QI=119|1|1|1|1|1|4|95|234 |
| chr2_1 | exon | 77793969 | 77794144 | 77793969 | ID=Msa0227140-mRNA-1:exon:23729;Parent=Msa0227140-mRNA-1 |
| chr2_1 | exon | 77794232 | 77794600 | 77794232 | ID=Msa0227140-mRNA-1:exon:23728;Parent=Msa0227140-mRNA-1 |
| chr2_1 | exon | 77796031 | 77796249 | 77796031 | ID=Msa0227140-mRNA-1:exon:23727;Parent=Msa0227140-mRNA-1 |
| chr2_1 | exon | 77796373 | 77796527 | 77796373 | ID=Msa0227140-mRNA-1:exon:23726;Parent=Msa0227140-mRNA-1 |
| chr2_1 | five_prime_UTR | 77796409 | 77796527 | 77796409 | ID=Msa0227140-mRNA-1:five_prime_utr;Parent=Msa0227140-mRNA-1 |
| chr2_1 | CDS | 77796373 | 77796408 | 77796373 | ID=Msa0227140-mRNA-1:cds;Parent=Msa0227140-mRNA-1 |
| chr2_1 | CDS | 77796031 | 77796249 | 77796031 | ID=Msa0227140-mRNA-1:cds;Parent=Msa0227140-mRNA-1 |
| chr2_1 | CDS | 77794232 | 77794600 | 77794232 | ID=Msa0227140-mRNA-1:cds;Parent=Msa0227140-mRNA-1 |
| chr2_1 | CDS | 77794064 | 77794144 | 77794064 | ID=Msa0227140-mRNA-1:cds;Parent=Msa0227140-mRNA-1 |
| chr2_1 | three_prime_UTR | 77793969 | 77794063 | 77793969 | ID=Msa0227140-mRNA-1:three_prime_utr;Parent=Msa0227140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
