AlfalfaGEDB | Msa0258330

M. sativa cultivar zhongmu-4 / Msa0258330

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF07876.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258330	XP_013464923.1	99.099	111	1	0	1	111	1	111	5.16e-71	217

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258330	sp\|Q9FK81\|Y5258_ARATH	65.657	99	34	0	7	105	6	104	5.42e-41	134

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258330	B7FMZ0	99.099	111	1	0	1	111	1	111	2.46e-71	217

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa0258330	0.801491	8.780102e-49	-8.615850e-47
Msa0036850	Msa0258330	0.804467	2.130178e-49	-8.615850e-47
Msa0078650	Msa0258330	0.807156	5.801099e-50	-8.615850e-47
Msa0081520	Msa0258330	0.803634	3.173865e-49	-8.615850e-47
Msa0110970	Msa0258330	0.831111	2.009290e-55	-8.615850e-47
Msa0157290	Msa0258330	0.802276	6.059497e-49	-8.615850e-47
Msa0171290	Msa0258330	0.819136	1.360964e-52	-8.615850e-47
Msa0218780	Msa0258330	0.821422	4.072901e-53	-8.615850e-47
Msa0239570	Msa0258330	0.814325	1.631981e-51	-8.615850e-47
Msa0258330	Msa0292400	0.805102	1.570022e-49	-8.615850e-47
Msa0258330	Msa0297740	0.928856	1.596485e-92	-8.615850e-47
Msa0258330	Msa0306300	0.840386	8.974395e-58	-8.615850e-47
Msa0258330	Msa0317280	0.800313	1.528123e-48	-8.615850e-47
Msa0258330	Msa0335780	0.991523	4.210751e-188	-8.615850e-47
Msa0258330	Msa0386850	0.801024	1.094579e-48	-8.615850e-47
Msa0258330	Msa0410650	0.802255	6.118146e-49	-8.615850e-47
Msa0258330	Msa0417880	0.805180	1.512051e-49	-8.615850e-47
Msa0258330	Msa0461720	0.801979	6.972195e-49	-8.615850e-47
Msa0258330	Msa0503580	0.821682	3.545578e-53	-8.615850e-47
Msa0258330	Msa0507680	0.806894	6.590865e-50	-8.615850e-47
Msa0258330	Msa0535040	0.800513	1.391413e-48	-8.615850e-47
Msa0258330	Msa0626450	0.802510	5.422690e-49	-8.615850e-47
Msa0258330	Msa0626490	0.803609	3.212542e-49	-8.615850e-47
Msa0258330	Msa0730430	0.812883	3.388791e-51	-8.615850e-47
Msa0258330	Msa0738040	0.814912	1.209740e-51	-8.615850e-47
Msa0258330	Msa0785210	0.818770	1.648153e-52	-8.615850e-47
Msa0258330	Msa0866270	0.816095	6.599266e-52	-8.615850e-47
Msa0258330	Msa0880140	0.800759	1.239466e-48	-8.615850e-47
Msa0258330	Msa1020670	0.819635	1.047219e-52	-8.615850e-47
Msa0258330	Msa1096970	0.820150	7.983704e-53	-8.615850e-47
Msa0258330	Msa1140210	0.807868	4.095169e-50	-8.615850e-47
Msa0258330	Msa1200020	0.827066	1.920471e-54	-8.615850e-47
Msa0258330	Msa1209460	0.807465	4.989574e-50	-8.615850e-47
Msa0258330	Msa1271990	0.811304	7.484340e-51	-8.615850e-47
Msa0258330	Msa1279380	0.838901	2.183288e-57	-8.615850e-47
Msa0258330	Msa1309790	0.800336	1.511914e-48	-8.615850e-47
Msa0258330	Msa1373330	0.812035	5.191475e-51	-8.615850e-47
Msa0258330	Msa1432950	0.806786	6.946374e-50	-8.615850e-47
Msa0258330	Msa1435890	0.800774	1.230838e-48	-8.615850e-47
Msa0258330	Msa1437940	0.807054	6.096880e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258330	MtrunA17_Chr2g0321051	99.099	111	1	0	1	111	1	111	4.75e-75	217

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258330	AT5G22580.1	65.657	99	34	0	7	105	6	104	5.51e-42	134
Msa0258330	AT3G17210.1	42.000	100	54	2	8	104	9	107	3.51e-22	84.0
Msa0258330	AT3G17210.2	42.000	100	54	2	8	104	9	107	3.51e-22	84.0

Find 47 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAATATATAAGCACAATTT+AGG	0.149609	2_2:-54582079	None:intergenic
GATTATGCTGCTTCCTTAAC+TGG	0.195704	2_2:-54583078	None:intergenic
CTAAATTGTGCTTATATATT+AGG	0.202013	2_2:+54582080	Msa0258330:five_prime_UTR
CTGTTAAATTATAGTAAAAT+TGG	0.209409	2_2:+54583418	Msa0258330:three_prime_UTR
AGAAGTTACATAATTGATTA+TGG	0.296856	2_2:+54581979	Msa0258330:five_prime_UTR
TACAAAAGGGATGGAGAAAT+TGG	0.322091	2_2:+54582204	Msa0258330:CDS
AATCATATCCATTGATTAGA+AGG	0.325835	2_2:-54583280	None:intergenic
AATAATGAATTATTTATTGT+TGG	0.369546	2_2:-54583119	None:intergenic
TAATATATAAGCACAATTTA+GGG	0.373646	2_2:-54582078	None:intergenic
AAGCTTTACTTCACAAAGAA+TGG	0.408647	2_2:+54582114	Msa0258330:exon
ATTTGTATGGTAAAAGATAA+GGG	0.415631	2_2:+54583253	Msa0258330:three_prime_UTR
AGGGTTTGCCTTCTAATCAA+TGG	0.422168	2_2:+54583272	Msa0258330:three_prime_UTR
ATCTTGTCAAATCCTTTGAA+TGG	0.422994	2_2:+54582239	Msa0258330:CDS
AACTGGCTTGACAAGGGTTG+AGG	0.423667	2_2:-54583061	None:intergenic
TGGACACACTTTGAATTTGA+AGG	0.429380	2_2:+54581954	Msa0258330:five_prime_UTR
AACCCTTGTCAAGCCAGTTA+AGG	0.431460	2_2:+54583065	Msa0258330:CDS
CTTCCTTAACTGGCTTGACA+AGG	0.454173	2_2:-54583068	None:intergenic
CTTGTTGAATGTCATCAAGA+AGG	0.457412	2_2:-54582941	None:intergenic
GTGATTGTTAAGTTCAAAGA+AGG	0.458164	2_2:+54582163	Msa0258330:CDS
GTGAAGTAAAGCTTGCTACT+AGG	0.467741	2_2:-54582105	None:intergenic
ATTACTGAACTACGAACAAA+AGG	0.471004	2_2:-54583188	None:intergenic
TATTTGTATGGTAAAAGATA+AGG	0.471222	2_2:+54583252	Msa0258330:three_prime_UTR
GTAGTGGAAGAGCTTACAAA+AGG	0.480357	2_2:+54582190	Msa0258330:CDS
GTTCAAAGAAGGTGTAGTAG+TGG	0.481023	2_2:+54582174	Msa0258330:CDS
GACTCATTTCATTACTATGT+TGG	0.483365	2_2:-54582002	None:intergenic
TCAGAGAACTCAACATGACT+TGG	0.494285	2_2:-54582994	None:intergenic
ACTGGCTTGACAAGGGTTGA+GGG	0.505995	2_2:-54583060	None:intergenic
AACCTTGTCTCAACACATCT+AGG	0.520013	2_2:-54582909	None:intergenic
AAGCACAATTTAGGGTTCAA+AGG	0.525734	2_2:-54582070	None:intergenic
ATAGTAATGAAATGAGTCGT+GGG	0.527643	2_2:+54582007	Msa0258330:five_prime_UTR
TCTGTACTGTGATATTTGTA+TGG	0.531843	2_2:+54583240	Msa0258330:three_prime_UTR
TTTCTAATCATACAGGGGAC+AGG	0.545053	2_2:+54582876	Msa0258330:intron
GGACACACTTTGAATTTGAA+GGG	0.549862	2_2:+54581955	Msa0258330:five_prime_UTR
TATTAGACGTTGACACATGT+TGG	0.550823	2_2:+54581927	None:intergenic
ATTGACAGATCAAGACAGTA+AGG	0.554102	2_2:+54582039	Msa0258330:five_prime_UTR
AGAACTCAACATGACTTGGA+TGG	0.554383	2_2:-54582990	None:intergenic
TTCACAAAGAATGGTAGTCA+TGG	0.559784	2_2:+54582123	Msa0258330:exon
TTCCTTAACTGGCTTGACAA+GGG	0.564730	2_2:-54583067	None:intergenic
CGTTGACACATGTTGGACAC+TGG	0.574307	2_2:+54581934	Msa0258330:five_prime_UTR
AGCCTAGATGTGTTGAGACA+AGG	0.579475	2_2:+54582907	Msa0258330:CDS
CTGGCTTGACAAGGGTTGAG+GGG	0.581401	2_2:-54583059	None:intergenic
TAGTGGAAGAGCTTACAAAA+GGG	0.588758	2_2:+54582191	Msa0258330:CDS
GGAAGAGCTTACAAAAGGGA+TGG	0.589362	2_2:+54582195	Msa0258330:CDS
CATAGTAATGAAATGAGTCG+TGG	0.608824	2_2:+54582006	Msa0258330:five_prime_UTR
GTATAAGACATACCATTCAA+AGG	0.611465	2_2:-54582251	None:intergenic
ACAAAGAATGGTAGTCATGG+AGG	0.636423	2_2:+54582126	Msa0258330:exon
TAGTAATGAAATGAGTCGTG+GGG	0.683523	2_2:+54582008	Msa0258330:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATACAATTATTTATATTGA+CGG	-	chr2_2:54582697-54582716	None:intergenic	10.0%
!!	AATAATGAATTATTTATTGT+TGG	-	chr2_2:54583122-54583141	None:intergenic	10.0%
!!	TATACAATTATTTATATTGA+CGG	-	chr2_2:54582697-54582716	None:intergenic	10.0%
!!	AATAATGAATTATTTATTGT+TGG	-	chr2_2:54583122-54583141	None:intergenic	10.0%
!!	TAATATATAAGCACAATTTA+GGG	-	chr2_2:54582081-54582100	None:intergenic	15.0%
!!!	ACATAAAAAAATTTGATTTC+AGG	+	chr2_2:54582604-54582623	Msa0258330:intron	15.0%
!!	AATTACTAGTTATAAGTAAT+CGG	+	chr2_2:54582759-54582778	Msa0258330:intron	15.0%
!!	CTGTTAAATTATAGTAAAAT+TGG	+	chr2_2:54583418-54583437	Msa0258330:three_prime_UTR	15.0%
!!	TAATATATAAGCACAATTTA+GGG	-	chr2_2:54582081-54582100	None:intergenic	15.0%
!!!	ACATAAAAAAATTTGATTTC+AGG	+	chr2_2:54582604-54582623	Msa0258330:intron	15.0%
!!	AATTACTAGTTATAAGTAAT+CGG	+	chr2_2:54582759-54582778	Msa0258330:intron	15.0%
!!	CTGTTAAATTATAGTAAAAT+TGG	+	chr2_2:54583418-54583437	Msa0258330:three_prime_UTR	15.0%
!!	AGAAGTTACATAATTGATTA+TGG	+	chr2_2:54581979-54581998	Msa0258330:five_prime_UTR	20.0%
!!	CTAATATATAAGCACAATTT+AGG	-	chr2_2:54582082-54582101	None:intergenic	20.0%
!!	CTAAATTGTGCTTATATATT+AGG	+	chr2_2:54582080-54582099	Msa0258330:five_prime_UTR	20.0%
!!	TATAAATCTCAGTAAAATCA+TGG	-	chr2_2:54582543-54582562	None:intergenic	20.0%
!!	ACTGAGATTTATAGTTAAAT+AGG	+	chr2_2:54582550-54582569	Msa0258330:intron	20.0%
!!	ATATGATAGTACTATAAAGA+GGG	-	chr2_2:54582849-54582868	None:intergenic	20.0%
!!	TATTTGTATGGTAAAAGATA+AGG	+	chr2_2:54583252-54583271	Msa0258330:three_prime_UTR	20.0%
!!	ATTTGTATGGTAAAAGATAA+GGG	+	chr2_2:54583253-54583272	Msa0258330:three_prime_UTR	20.0%
!!	AGAAGTTACATAATTGATTA+TGG	+	chr2_2:54581979-54581998	Msa0258330:five_prime_UTR	20.0%
!!	CTAATATATAAGCACAATTT+AGG	-	chr2_2:54582082-54582101	None:intergenic	20.0%
!!	CTAAATTGTGCTTATATATT+AGG	+	chr2_2:54582080-54582099	Msa0258330:five_prime_UTR	20.0%
!!	TATAAATCTCAGTAAAATCA+TGG	-	chr2_2:54582543-54582562	None:intergenic	20.0%
!!	ACTGAGATTTATAGTTAAAT+AGG	+	chr2_2:54582550-54582569	Msa0258330:intron	20.0%
!!	ATATGATAGTACTATAAAGA+GGG	-	chr2_2:54582849-54582868	None:intergenic	20.0%
!!	TATTTGTATGGTAAAAGATA+AGG	+	chr2_2:54583252-54583271	Msa0258330:three_prime_UTR	20.0%
!!	ATTTGTATGGTAAAAGATAA+GGG	+	chr2_2:54583253-54583272	Msa0258330:three_prime_UTR	20.0%
!	ATCAAAGGTTAATAAACTGA+AGG	-	chr2_2:54582351-54582370	None:intergenic	25.0%
!	AAAAACACTATCTGTATCAA+AGG	-	chr2_2:54582366-54582385	None:intergenic	25.0%
!	TCTGTTATCTAATATATCAG+TGG	-	chr2_2:54582400-54582419	None:intergenic	25.0%
!	AAACAGAAATACAAGAACAA+GGG	+	chr2_2:54582419-54582438	Msa0258330:intron	25.0%
!	TCTCATGTACTTAAATATCA+AGG	+	chr2_2:54582474-54582493	Msa0258330:intron	25.0%
!!!	AATATCAAGGTTTTAGATTG+AGG	+	chr2_2:54582487-54582506	Msa0258330:intron	25.0%
!!!	ATTTTTTTATGTTTTCGCGT+TGG	-	chr2_2:54582596-54582615	None:intergenic	25.0%
!	ATGTGACGATTATGATTATT+TGG	+	chr2_2:54582798-54582817	Msa0258330:intron	25.0%
!	GATATGATAGTACTATAAAG+AGG	-	chr2_2:54582850-54582869	None:intergenic	25.0%
!	ATAAGCTTTCTAATCATACA+GGG	+	chr2_2:54582870-54582889	Msa0258330:intron	25.0%
!!	AATCATATCCATTGATTAGA+AGG	-	chr2_2:54583283-54583302	None:intergenic	25.0%
!	ATCAAAGGTTAATAAACTGA+AGG	-	chr2_2:54582351-54582370	None:intergenic	25.0%
!	AAAAACACTATCTGTATCAA+AGG	-	chr2_2:54582366-54582385	None:intergenic	25.0%
!	TCTGTTATCTAATATATCAG+TGG	-	chr2_2:54582400-54582419	None:intergenic	25.0%
!	AAACAGAAATACAAGAACAA+GGG	+	chr2_2:54582419-54582438	Msa0258330:intron	25.0%
!	TCTCATGTACTTAAATATCA+AGG	+	chr2_2:54582474-54582493	Msa0258330:intron	25.0%
!!!	AATATCAAGGTTTTAGATTG+AGG	+	chr2_2:54582487-54582506	Msa0258330:intron	25.0%
!!!	ATTTTTTTATGTTTTCGCGT+TGG	-	chr2_2:54582596-54582615	None:intergenic	25.0%
!	ATGTGACGATTATGATTATT+TGG	+	chr2_2:54582798-54582817	Msa0258330:intron	25.0%
!	GATATGATAGTACTATAAAG+AGG	-	chr2_2:54582850-54582869	None:intergenic	25.0%
!	ATAAGCTTTCTAATCATACA+GGG	+	chr2_2:54582870-54582889	Msa0258330:intron	25.0%
!!	AATCATATCCATTGATTAGA+AGG	-	chr2_2:54583283-54583302	None:intergenic	25.0%
	GACTCATTTCATTACTATGT+TGG	-	chr2_2:54582005-54582024	None:intergenic	30.0%
	ATAGTAATGAAATGAGTCGT+GGG	+	chr2_2:54582007-54582026	Msa0258330:five_prime_UTR	30.0%
	AAGCTTTACTTCACAAAGAA+TGG	+	chr2_2:54582114-54582133	Msa0258330:exon	30.0%
	GTGATTGTTAAGTTCAAAGA+AGG	+	chr2_2:54582163-54582182	Msa0258330:CDS	30.0%
	ATCTTGTCAAATCCTTTGAA+TGG	+	chr2_2:54582239-54582258	Msa0258330:CDS	30.0%
	GTATAAGACATACCATTCAA+AGG	-	chr2_2:54582254-54582273	None:intergenic	30.0%
!	TACCTTTTATATTGTGCAAG+AGG	+	chr2_2:54582277-54582296	Msa0258330:intron	30.0%
	GAAACAGAAATACAAGAACA+AGG	+	chr2_2:54582418-54582437	Msa0258330:intron	30.0%
	CATAAGCTTTCTAATCATAC+AGG	+	chr2_2:54582869-54582888	Msa0258330:intron	30.0%
	TAAGCTTTCTAATCATACAG+GGG	+	chr2_2:54582871-54582890	Msa0258330:intron	30.0%
	ATTACTGAACTACGAACAAA+AGG	-	chr2_2:54583191-54583210	None:intergenic	30.0%
	TCTGTACTGTGATATTTGTA+TGG	+	chr2_2:54583240-54583259	Msa0258330:three_prime_UTR	30.0%
	GACTCATTTCATTACTATGT+TGG	-	chr2_2:54582005-54582024	None:intergenic	30.0%
	ATAGTAATGAAATGAGTCGT+GGG	+	chr2_2:54582007-54582026	Msa0258330:five_prime_UTR	30.0%
	AAGCTTTACTTCACAAAGAA+TGG	+	chr2_2:54582114-54582133	Msa0258330:exon	30.0%
	GTGATTGTTAAGTTCAAAGA+AGG	+	chr2_2:54582163-54582182	Msa0258330:CDS	30.0%
	ATCTTGTCAAATCCTTTGAA+TGG	+	chr2_2:54582239-54582258	Msa0258330:CDS	30.0%
	GTATAAGACATACCATTCAA+AGG	-	chr2_2:54582254-54582273	None:intergenic	30.0%
!	TACCTTTTATATTGTGCAAG+AGG	+	chr2_2:54582277-54582296	Msa0258330:intron	30.0%
	GAAACAGAAATACAAGAACA+AGG	+	chr2_2:54582418-54582437	Msa0258330:intron	30.0%
	CATAAGCTTTCTAATCATAC+AGG	+	chr2_2:54582869-54582888	Msa0258330:intron	30.0%
	TAAGCTTTCTAATCATACAG+GGG	+	chr2_2:54582871-54582890	Msa0258330:intron	30.0%
	ATTACTGAACTACGAACAAA+AGG	-	chr2_2:54583191-54583210	None:intergenic	30.0%
	TCTGTACTGTGATATTTGTA+TGG	+	chr2_2:54583240-54583259	Msa0258330:three_prime_UTR	30.0%
	TGGACACACTTTGAATTTGA+AGG	+	chr2_2:54581954-54581973	Msa0258330:five_prime_UTR	35.0%
	GGACACACTTTGAATTTGAA+GGG	+	chr2_2:54581955-54581974	Msa0258330:five_prime_UTR	35.0%
	CATAGTAATGAAATGAGTCG+TGG	+	chr2_2:54582006-54582025	Msa0258330:five_prime_UTR	35.0%
	TAGTAATGAAATGAGTCGTG+GGG	+	chr2_2:54582008-54582027	Msa0258330:five_prime_UTR	35.0%
	ATTGACAGATCAAGACAGTA+AGG	+	chr2_2:54582039-54582058	Msa0258330:five_prime_UTR	35.0%
!	AAGCACAATTTAGGGTTCAA+AGG	-	chr2_2:54582073-54582092	None:intergenic	35.0%
	TTCACAAAGAATGGTAGTCA+TGG	+	chr2_2:54582123-54582142	Msa0258330:exon	35.0%
	TAGTGGAAGAGCTTACAAAA+GGG	+	chr2_2:54582191-54582210	Msa0258330:CDS	35.0%
	TACAAAAGGGATGGAGAAAT+TGG	+	chr2_2:54582204-54582223	Msa0258330:CDS	35.0%
	AGCCTCTTGCACAATATAAA+AGG	-	chr2_2:54582282-54582301	None:intergenic	35.0%
	TTGATTTCAGGCTTACATCA+CGG	+	chr2_2:54582616-54582635	Msa0258330:intron	35.0%
	CTTGTTGAATGTCATCAAGA+AGG	-	chr2_2:54582944-54582963	None:intergenic	35.0%
	TGGACACACTTTGAATTTGA+AGG	+	chr2_2:54581954-54581973	Msa0258330:five_prime_UTR	35.0%
	GGACACACTTTGAATTTGAA+GGG	+	chr2_2:54581955-54581974	Msa0258330:five_prime_UTR	35.0%
	CATAGTAATGAAATGAGTCG+TGG	+	chr2_2:54582006-54582025	Msa0258330:five_prime_UTR	35.0%
	TAGTAATGAAATGAGTCGTG+GGG	+	chr2_2:54582008-54582027	Msa0258330:five_prime_UTR	35.0%
	ATTGACAGATCAAGACAGTA+AGG	+	chr2_2:54582039-54582058	Msa0258330:five_prime_UTR	35.0%
!	AAGCACAATTTAGGGTTCAA+AGG	-	chr2_2:54582073-54582092	None:intergenic	35.0%
	TTCACAAAGAATGGTAGTCA+TGG	+	chr2_2:54582123-54582142	Msa0258330:exon	35.0%
	TAGTGGAAGAGCTTACAAAA+GGG	+	chr2_2:54582191-54582210	Msa0258330:CDS	35.0%
	TACAAAAGGGATGGAGAAAT+TGG	+	chr2_2:54582204-54582223	Msa0258330:CDS	35.0%
	AGCCTCTTGCACAATATAAA+AGG	-	chr2_2:54582282-54582301	None:intergenic	35.0%
	TTGATTTCAGGCTTACATCA+CGG	+	chr2_2:54582616-54582635	Msa0258330:intron	35.0%
	CTTGTTGAATGTCATCAAGA+AGG	-	chr2_2:54582944-54582963	None:intergenic	35.0%
!!	GTGAAGTAAAGCTTGCTACT+AGG	-	chr2_2:54582108-54582127	None:intergenic	40.0%
	ACAAAGAATGGTAGTCATGG+AGG	+	chr2_2:54582126-54582145	Msa0258330:exon	40.0%
!	GTTCAAAGAAGGTGTAGTAG+TGG	+	chr2_2:54582174-54582193	Msa0258330:CDS	40.0%
!	GTAGTGGAAGAGCTTACAAA+AGG	+	chr2_2:54582190-54582209	Msa0258330:CDS	40.0%
	AGTTAAATAGGCCTCGATTG+TGG	+	chr2_2:54582562-54582581	Msa0258330:intron	40.0%
!!!	ATGTTTTCGCGTTGGTAATG+TGG	-	chr2_2:54582588-54582607	None:intergenic	40.0%
	TTTCTAATCATACAGGGGAC+AGG	+	chr2_2:54582876-54582895	Msa0258330:intron	40.0%
	AACCTTGTCTCAACACATCT+AGG	-	chr2_2:54582912-54582931	None:intergenic	40.0%
!	AGAACTCAACATGACTTGGA+TGG	-	chr2_2:54582993-54583012	None:intergenic	40.0%
	TCAGAGAACTCAACATGACT+TGG	-	chr2_2:54582997-54583016	None:intergenic	40.0%
!!	TTCCTTAACTGGCTTGACAA+GGG	-	chr2_2:54583070-54583089	None:intergenic	40.0%
!	GATTATGCTGCTTCCTTAAC+TGG	-	chr2_2:54583081-54583100	None:intergenic	40.0%
	AGGGTTTGCCTTCTAATCAA+TGG	+	chr2_2:54583272-54583291	Msa0258330:three_prime_UTR	40.0%
!!	GTGAAGTAAAGCTTGCTACT+AGG	-	chr2_2:54582108-54582127	None:intergenic	40.0%
	ACAAAGAATGGTAGTCATGG+AGG	+	chr2_2:54582126-54582145	Msa0258330:exon	40.0%
!	GTTCAAAGAAGGTGTAGTAG+TGG	+	chr2_2:54582174-54582193	Msa0258330:CDS	40.0%
!	GTAGTGGAAGAGCTTACAAA+AGG	+	chr2_2:54582190-54582209	Msa0258330:CDS	40.0%
	AGTTAAATAGGCCTCGATTG+TGG	+	chr2_2:54582562-54582581	Msa0258330:intron	40.0%
!!!	ATGTTTTCGCGTTGGTAATG+TGG	-	chr2_2:54582588-54582607	None:intergenic	40.0%
	TTTCTAATCATACAGGGGAC+AGG	+	chr2_2:54582876-54582895	Msa0258330:intron	40.0%
	AACCTTGTCTCAACACATCT+AGG	-	chr2_2:54582912-54582931	None:intergenic	40.0%
!	AGAACTCAACATGACTTGGA+TGG	-	chr2_2:54582993-54583012	None:intergenic	40.0%
	TCAGAGAACTCAACATGACT+TGG	-	chr2_2:54582997-54583016	None:intergenic	40.0%
!!	TTCCTTAACTGGCTTGACAA+GGG	-	chr2_2:54583070-54583089	None:intergenic	40.0%
!	GATTATGCTGCTTCCTTAAC+TGG	-	chr2_2:54583081-54583100	None:intergenic	40.0%
	AGGGTTTGCCTTCTAATCAA+TGG	+	chr2_2:54583272-54583291	Msa0258330:three_prime_UTR	40.0%
	GGAAGAGCTTACAAAAGGGA+TGG	+	chr2_2:54582195-54582214	Msa0258330:CDS	45.0%
	AGCCTAGATGTGTTGAGACA+AGG	+	chr2_2:54582907-54582926	Msa0258330:CDS	45.0%
	AACCCTTGTCAAGCCAGTTA+AGG	+	chr2_2:54583065-54583084	Msa0258330:CDS	45.0%
!!	CTTCCTTAACTGGCTTGACA+AGG	-	chr2_2:54583071-54583090	None:intergenic	45.0%
	GGAAGAGCTTACAAAAGGGA+TGG	+	chr2_2:54582195-54582214	Msa0258330:CDS	45.0%
	AGCCTAGATGTGTTGAGACA+AGG	+	chr2_2:54582907-54582926	Msa0258330:CDS	45.0%
	AACCCTTGTCAAGCCAGTTA+AGG	+	chr2_2:54583065-54583084	Msa0258330:CDS	45.0%
!!	CTTCCTTAACTGGCTTGACA+AGG	-	chr2_2:54583071-54583090	None:intergenic	45.0%
	CGTTGACACATGTTGGACAC+TGG	+	chr2_2:54581934-54581953	Msa0258330:five_prime_UTR	50.0%
!	TGGTAATGTGGCCACAATCG+AGG	-	chr2_2:54582576-54582595	None:intergenic	50.0%
!!	ACTGGCTTGACAAGGGTTGA+GGG	-	chr2_2:54583063-54583082	None:intergenic	50.0%
!!	AACTGGCTTGACAAGGGTTG+AGG	-	chr2_2:54583064-54583083	None:intergenic	50.0%
	CGTTGACACATGTTGGACAC+TGG	+	chr2_2:54581934-54581953	Msa0258330:five_prime_UTR	50.0%
!	TGGTAATGTGGCCACAATCG+AGG	-	chr2_2:54582576-54582595	None:intergenic	50.0%
!!	ACTGGCTTGACAAGGGTTGA+GGG	-	chr2_2:54583063-54583082	None:intergenic	50.0%
!!	AACTGGCTTGACAAGGGTTG+AGG	-	chr2_2:54583064-54583083	None:intergenic	50.0%
	CGGTCGTAGACTCGTAGTTG+TGG	+	chr2_2:54582636-54582655	Msa0258330:intron	55.0%
!	CTGGCTTGACAAGGGTTGAG+GGG	-	chr2_2:54583062-54583081	None:intergenic	55.0%
	CGGTCGTAGACTCGTAGTTG+TGG	+	chr2_2:54582636-54582655	Msa0258330:intron	55.0%
!	CTGGCTTGACAAGGGTTGAG+GGG	-	chr2_2:54583062-54583081	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	54581929	54583446	54581929	ID=Msa0258330;Name=Msa0258330
chr2_2	mRNA	54581929	54583446	54581929	ID=Msa0258330-mRNA-1;Parent=Msa0258330;Name=Msa0258330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=204\|1\|1\|1\|1\|1\|2\|348\|111
chr2_2	exon	54581929	54582260	54581929	ID=Msa0258330-mRNA-1:exon:16387;Parent=Msa0258330-mRNA-1
chr2_2	exon	54582891	54583446	54582891	ID=Msa0258330-mRNA-1:exon:16388;Parent=Msa0258330-mRNA-1
chr2_2	five_prime_UTR	54581929	54582132	54581929	ID=Msa0258330-mRNA-1:five_prime_utr;Parent=Msa0258330-mRNA-1
chr2_2	CDS	54582133	54582260	54582133	ID=Msa0258330-mRNA-1:cds;Parent=Msa0258330-mRNA-1
chr2_2	CDS	54582891	54583098	54582891	ID=Msa0258330-mRNA-1:cds;Parent=Msa0258330-mRNA-1
chr2_2	three_prime_UTR	54583099	54583446	54583099	ID=Msa0258330-mRNA-1:three_prime_utr;Parent=Msa0258330-mRNA-1

Gene Sequence	>Msa0258330 ATGGTAGTCATGGAGGAATTCAAGCACTTTGTGATTGTTAAGTTCAAAGAAGGTGTAGTAGTGGAAGAGCTTACAAAAGGGATGGAGAAATTGGTCTCAGAGATTGATCTTGTCAAATCCTTTGAATGGGGACAGGATATTGAAAGCCTAGATGTGTTGAGACAAGGTTTCACTCATGCCTTCTTGATGACATTCAACAAGAAAGAAGATTTTGCTGCATTTCAGAGCCATCCAAGTCATGTTGAGTTCTCTGAAAAGTTTTCAACAGCTATTGAAAAGATTGTGCTGCTTGATTTCCCCTCAACCCTTGTCAAGCCAGTTAAGGAAGCAGCATAA
Protein sequence	>Msa0258330 MVVMEEFKHFVIVKFKEGVVVEELTKGMEKLVSEIDLVKSFEWGQDIESLDVLRQGFTHAFLMTFNKKEDFAAFQSHPSHVEFSEKFSTAIEKIVLLDFPSTLVKPVKEAA*