AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1294120


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1294120 MtrunA17_Chr8g0370231 96.970 66 2 0 3 68 869 934 2.31e-39 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1294120 AT2G17510.2 75.758 66 16 0 3 68 947 1012 5.39e-32 116
Msa1294120 AT2G17510.1 75.758 66 16 0 3 68 868 933 6.11e-32 115

Find 32 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAACCCTATATATCAAAA+TGG 0.336251 8_3:+51825573 None:intergenic
GATACACGTTGGCTTAAATA+TGG 0.339259 8_3:-51825530 Msa1294120:three_prime_UTR
ACCTCCGCACTTGAAGTTTC+TGG 0.344839 8_3:-51825782 Msa1294120:three_prime_UTR
TGTCACAGATTCGGCATAGA+AGG 0.357548 8_3:-51826068 Msa1294120:intron
GCTCTTGTTGTCAAATATAC+AGG 0.379477 8_3:+51826044 None:intergenic
TTTGACAACAAGAGCTGAAA+AGG 0.403110 8_3:-51826036 Msa1294120:CDS
AGCTGAAGCTTTGGCCGGTT+AGG 0.424906 8_3:+51825897 None:intergenic
CTCTCATCTAGAGGTATACT+AGG 0.448622 8_3:-51825872 Msa1294120:exon
ACAAATTCACTTGGAGGTTG+TGG 0.454352 8_3:-51825928 Msa1294120:CDS
TTATCGTATAACCCATGAAA+AGG 0.468469 8_3:-51826233 None:intergenic
TTGTGGAGCCTCAACCTAAC+CGG 0.471178 8_3:-51825911 Msa1294120:CDS
AGCTTTGGCCGGTTAGGTTG+AGG 0.480175 8_3:+51825903 None:intergenic
ATGAGAGTAAGCTGAAGCTT+TGG 0.481186 8_3:+51825888 None:intergenic
GAGCAATATAAATAAATAAG+AGG 0.483680 8_3:+51825810 None:intergenic
CTGAAAAGGGTAGCGGAGAA+TGG 0.489319 8_3:-51826022 Msa1294120:CDS
GCTTGAGTAGTAGTGTTGCT+TGG 0.510618 8_3:-51825679 Msa1294120:three_prime_UTR
CATTGAACAAGAAGCAAGCT+AGG 0.535530 8_3:-51825840 Msa1294120:three_prime_UTR
GACGGTACAAATTCACTTGG+AGG 0.540234 8_3:-51825934 Msa1294120:CDS
GGTATTAGAATTGACTGGCA+TGG 0.541030 8_3:-51825761 Msa1294120:three_prime_UTR
TAGTTTCGTAAGTGTGAATG+AGG 0.546738 8_3:-51825602 Msa1294120:three_prime_UTR
AAACTTCAAGTGCGGAGGTG+TGG 0.547763 8_3:+51825786 None:intergenic
CAACACTACTACTCAAGCTA+TGG 0.553424 8_3:+51825684 None:intergenic
TTTCTGGTATTAGAATTGAC+TGG 0.567157 8_3:-51825766 Msa1294120:three_prime_UTR
TTGACAACAAGAGCTGAAAA+GGG 0.568719 8_3:-51826035 Msa1294120:CDS
GCAGACGGTACAAATTCACT+TGG 0.575230 8_3:-51825937 Msa1294120:CDS
AATCTCTCTCAAGTGCAAGA+TGG 0.577628 8_3:+51825504 None:intergenic
GAGTAAGCTGAAGCTTTGGC+CGG 0.579724 8_3:+51825892 None:intergenic
TTTATCAAAATGATACACGT+TGG 0.591318 8_3:-51825541 Msa1294120:three_prime_UTR
ACAAGAGCTGAAAAGGGTAG+CGG 0.620766 8_3:-51826029 Msa1294120:CDS
TTCAGCTTACTCTCATCTAG+AGG 0.635613 8_3:-51825881 Msa1294120:exon
ACCAGAAACTTCAAGTGCGG+AGG 0.640511 8_3:+51825781 None:intergenic
AATACCAGAAACTTCAAGTG+CGG 0.692524 8_3:+51825778 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAGCAATATAAATAAATAAG+AGG + chr8_3:51825847-51825866 None:intergenic 20.0%
!!! AATTGTCCATTTTGATATAT+AGG - chr8_3:51826075-51826094 Msa1294120:intron 20.0%
!!! ATTGTCCATTTTGATATATA+GGG - chr8_3:51826076-51826095 Msa1294120:intron 20.0%
!! GAGCAATATAAATAAATAAG+AGG + chr8_3:51825847-51825866 None:intergenic 20.0%
!!! AATTGTCCATTTTGATATAT+AGG - chr8_3:51826075-51826094 Msa1294120:intron 20.0%
!!! ATTGTCCATTTTGATATATA+GGG - chr8_3:51826076-51826095 Msa1294120:intron 20.0%
! GTACTATTAATATTACGAGA+TGG - chr8_3:51825466-51825485 Msa1294120:three_prime_UTR 25.0%
! ACTAACCCTATATATCAAAA+TGG + chr8_3:51826084-51826103 None:intergenic 25.0%
! TTTATCAAAATGATACACGT+TGG - chr8_3:51826113-51826132 Msa1294120:intron 25.0%
!!! GTATAACTGTTTTGATTAGT+TGG - chr8_3:51826174-51826193 Msa1294120:intron 25.0%
! GTACTATTAATATTACGAGA+TGG - chr8_3:51825466-51825485 Msa1294120:three_prime_UTR 25.0%
! ACTAACCCTATATATCAAAA+TGG + chr8_3:51826084-51826103 None:intergenic 25.0%
! TTTATCAAAATGATACACGT+TGG - chr8_3:51826113-51826132 Msa1294120:intron 25.0%
!!! GTATAACTGTTTTGATTAGT+TGG - chr8_3:51826174-51826193 Msa1294120:intron 25.0%
!!! CTTTTTTCTTGTCACAGATT+CGG - chr8_3:51825577-51825596 Msa1294120:three_prime_UTR 30.0%
AGAGCAGAAGATTAAAAAGA+CGG - chr8_3:51825669-51825688 Msa1294120:three_prime_UTR 30.0%
! TTTCTGGTATTAGAATTGAC+TGG - chr8_3:51825888-51825907 Msa1294120:CDS 30.0%
! ATTTTGTTGGAAGAATTGAC+TGG - chr8_3:51825918-51825937 Msa1294120:CDS 30.0%
!!! CTTTTTTCTTGTCACAGATT+CGG - chr8_3:51825577-51825596 Msa1294120:three_prime_UTR 30.0%
AGAGCAGAAGATTAAAAAGA+CGG - chr8_3:51825669-51825688 Msa1294120:three_prime_UTR 30.0%
! TTTCTGGTATTAGAATTGAC+TGG - chr8_3:51825888-51825907 Msa1294120:CDS 30.0%
! ATTTTGTTGGAAGAATTGAC+TGG - chr8_3:51825918-51825937 Msa1294120:CDS 30.0%
AGATGGTTACTCATTTCAGA+AGG - chr8_3:51825483-51825502 Msa1294120:three_prime_UTR 35.0%
! GCTCTTGTTGTCAAATATAC+AGG + chr8_3:51825613-51825632 None:intergenic 35.0%
TTTGACAACAAGAGCTGAAA+AGG - chr8_3:51825618-51825637 Msa1294120:three_prime_UTR 35.0%
TTGACAACAAGAGCTGAAAA+GGG - chr8_3:51825619-51825638 Msa1294120:three_prime_UTR 35.0%
CAGAAGATTAAAAAGACGGA+TGG - chr8_3:51825673-51825692 Msa1294120:three_prime_UTR 35.0%
! TTCATACAGCATTTTGCAGA+CGG - chr8_3:51825702-51825721 Msa1294120:three_prime_UTR 35.0%
AATACCAGAAACTTCAAGTG+CGG + chr8_3:51825879-51825898 None:intergenic 35.0%
! GACTGGCATAGATATTTTGT+TGG - chr8_3:51825935-51825954 Msa1294120:CDS 35.0%
TAGTTTCGTAAGTGTGAATG+AGG - chr8_3:51826052-51826071 Msa1294120:CDS 35.0%
!! GATACACGTTGGCTTAAATA+TGG - chr8_3:51826124-51826143 Msa1294120:intron 35.0%
AGATGGTTACTCATTTCAGA+AGG - chr8_3:51825483-51825502 Msa1294120:three_prime_UTR 35.0%
! GCTCTTGTTGTCAAATATAC+AGG + chr8_3:51825613-51825632 None:intergenic 35.0%
TTTGACAACAAGAGCTGAAA+AGG - chr8_3:51825618-51825637 Msa1294120:three_prime_UTR 35.0%
TTGACAACAAGAGCTGAAAA+GGG - chr8_3:51825619-51825638 Msa1294120:three_prime_UTR 35.0%
CAGAAGATTAAAAAGACGGA+TGG - chr8_3:51825673-51825692 Msa1294120:three_prime_UTR 35.0%
! TTCATACAGCATTTTGCAGA+CGG - chr8_3:51825702-51825721 Msa1294120:three_prime_UTR 35.0%
AATACCAGAAACTTCAAGTG+CGG + chr8_3:51825879-51825898 None:intergenic 35.0%
! GACTGGCATAGATATTTTGT+TGG - chr8_3:51825935-51825954 Msa1294120:CDS 35.0%
TAGTTTCGTAAGTGTGAATG+AGG - chr8_3:51826052-51826071 Msa1294120:CDS 35.0%
!! GATACACGTTGGCTTAAATA+TGG - chr8_3:51826124-51826143 Msa1294120:intron 35.0%
ACAAATTCACTTGGAGGTTG+TGG - chr8_3:51825726-51825745 Msa1294120:three_prime_UTR 40.0%
ATGAGAGTAAGCTGAAGCTT+TGG + chr8_3:51825769-51825788 None:intergenic 40.0%
TTCAGCTTACTCTCATCTAG+AGG - chr8_3:51825773-51825792 Msa1294120:three_prime_UTR 40.0%
! CTCTCATCTAGAGGTATACT+AGG - chr8_3:51825782-51825801 Msa1294120:three_prime_UTR 40.0%
CATTGAACAAGAAGCAAGCT+AGG - chr8_3:51825814-51825833 Msa1294120:three_prime_UTR 40.0%
GGTATTAGAATTGACTGGCA+TGG - chr8_3:51825893-51825912 Msa1294120:CDS 40.0%
! GACTGGCATGGATATTTTGT+TGG - chr8_3:51825905-51825924 Msa1294120:CDS 40.0%
CAACACTACTACTCAAGCTA+TGG + chr8_3:51825973-51825992 None:intergenic 40.0%
AATCTCTCTCAAGTGCAAGA+TGG + chr8_3:51826153-51826172 None:intergenic 40.0%
ACAAATTCACTTGGAGGTTG+TGG - chr8_3:51825726-51825745 Msa1294120:three_prime_UTR 40.0%
ATGAGAGTAAGCTGAAGCTT+TGG + chr8_3:51825769-51825788 None:intergenic 40.0%
TTCAGCTTACTCTCATCTAG+AGG - chr8_3:51825773-51825792 Msa1294120:three_prime_UTR 40.0%
! CTCTCATCTAGAGGTATACT+AGG - chr8_3:51825782-51825801 Msa1294120:three_prime_UTR 40.0%
CATTGAACAAGAAGCAAGCT+AGG - chr8_3:51825814-51825833 Msa1294120:three_prime_UTR 40.0%
GGTATTAGAATTGACTGGCA+TGG - chr8_3:51825893-51825912 Msa1294120:CDS 40.0%
! GACTGGCATGGATATTTTGT+TGG - chr8_3:51825905-51825924 Msa1294120:CDS 40.0%
CAACACTACTACTCAAGCTA+TGG + chr8_3:51825973-51825992 None:intergenic 40.0%
AATCTCTCTCAAGTGCAAGA+TGG + chr8_3:51826153-51826172 None:intergenic 40.0%
TGTCACAGATTCGGCATAGA+AGG - chr8_3:51825586-51825605 Msa1294120:three_prime_UTR 45.0%
ACAAGAGCTGAAAAGGGTAG+CGG - chr8_3:51825625-51825644 Msa1294120:three_prime_UTR 45.0%
GCAGACGGTACAAATTCACT+TGG - chr8_3:51825717-51825736 Msa1294120:three_prime_UTR 45.0%
GACGGTACAAATTCACTTGG+AGG - chr8_3:51825720-51825739 Msa1294120:three_prime_UTR 45.0%
! GCTTGAGTAGTAGTGTTGCT+TGG - chr8_3:51825975-51825994 Msa1294120:CDS 45.0%
TGTCACAGATTCGGCATAGA+AGG - chr8_3:51825586-51825605 Msa1294120:three_prime_UTR 45.0%
ACAAGAGCTGAAAAGGGTAG+CGG - chr8_3:51825625-51825644 Msa1294120:three_prime_UTR 45.0%
GCAGACGGTACAAATTCACT+TGG - chr8_3:51825717-51825736 Msa1294120:three_prime_UTR 45.0%
GACGGTACAAATTCACTTGG+AGG - chr8_3:51825720-51825739 Msa1294120:three_prime_UTR 45.0%
! GCTTGAGTAGTAGTGTTGCT+TGG - chr8_3:51825975-51825994 Msa1294120:CDS 45.0%
!! CTGAAAAGGGTAGCGGAGAA+TGG - chr8_3:51825632-51825651 Msa1294120:three_prime_UTR 50.0%
TTGTGGAGCCTCAACCTAAC+CGG - chr8_3:51825743-51825762 Msa1294120:three_prime_UTR 50.0%
! GAGTAAGCTGAAGCTTTGGC+CGG + chr8_3:51825765-51825784 None:intergenic 50.0%
AAACTTCAAGTGCGGAGGTG+TGG + chr8_3:51825871-51825890 None:intergenic 50.0%
ACCTCCGCACTTGAAGTTTC+TGG - chr8_3:51825872-51825891 Msa1294120:exon 50.0%
ACCAGAAACTTCAAGTGCGG+AGG + chr8_3:51825876-51825895 None:intergenic 50.0%
!! CTGAAAAGGGTAGCGGAGAA+TGG - chr8_3:51825632-51825651 Msa1294120:three_prime_UTR 50.0%
TTGTGGAGCCTCAACCTAAC+CGG - chr8_3:51825743-51825762 Msa1294120:three_prime_UTR 50.0%
! GAGTAAGCTGAAGCTTTGGC+CGG + chr8_3:51825765-51825784 None:intergenic 50.0%
AAACTTCAAGTGCGGAGGTG+TGG + chr8_3:51825871-51825890 None:intergenic 50.0%
ACCTCCGCACTTGAAGTTTC+TGG - chr8_3:51825872-51825891 Msa1294120:exon 50.0%
ACCAGAAACTTCAAGTGCGG+AGG + chr8_3:51825876-51825895 None:intergenic 50.0%
! AGCTTTGGCCGGTTAGGTTG+AGG + chr8_3:51825754-51825773 None:intergenic 55.0%
! AGCTGAAGCTTTGGCCGGTT+AGG + chr8_3:51825760-51825779 None:intergenic 55.0%
! AGCTTTGGCCGGTTAGGTTG+AGG + chr8_3:51825754-51825773 None:intergenic 55.0%
! AGCTGAAGCTTTGGCCGGTT+AGG + chr8_3:51825760-51825779 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 51825435 51826241 51825435 ID=Msa1294120;Name=Msa1294120
chr8_3 mRNA 51825435 51826241 51825435 ID=Msa1294120-mRNA-1;Parent=Msa1294120;Name=Msa1294120-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0.5|0.5|1|1|2|449|68
chr8_3 exon 51825435 51826082 51825435 ID=Msa1294120-mRNA-1:exon:14588;Parent=Msa1294120-mRNA-1
chr8_3 exon 51826234 51826241 51826234 ID=Msa1294120-mRNA-1:exon:14587;Parent=Msa1294120-mRNA-1
chr8_3 CDS 51826234 51826241 51826234 ID=Msa1294120-mRNA-1:cds;Parent=Msa1294120-mRNA-1
chr8_3 CDS 51825884 51826082 51825884 ID=Msa1294120-mRNA-1:cds;Parent=Msa1294120-mRNA-1
chr8_3 three_prime_UTR 51825435 51825883 51825435 ID=Msa1294120-mRNA-1:three_prime_utr;Parent=Msa1294120-mRNA-1
Gene Sequence

>Msa1294120

ATGAAAAGATTCGGCATAGAAGGACCTGTATATTTGACAACAAGAGCTGAAAAGGGTAGCGGAGAATGGTATGTAGATGAACAAGAGCAGAAGATTAAAAAGACGGATGGTAGCTTTTCATACAGCATTTTGCAGACGGTACAAATTCACTTGGAGGTTGTGGAGCCTCAACCTAACCGGCCAAAGCTTCAGCTTACTCTCATCTAG

Protein sequence

>Msa1294120

MKRFGIEGPVYLTTRAEKGSGEWYVDEQEQKIKKTDGSFSYSILQTVQIHLEVVEPQPNRPKLQLTLI*