AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0009430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0009430 MtrunA17_Chr8g0370231 96.970 66 2 0 7 72 869 934 1.73e-39 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0009430 AT2G17510.2 75.758 66 16 0 7 72 947 1012 5.74e-32 116
Msa0009430 AT2G17510.1 75.758 66 16 0 7 72 868 933 6.57e-32 116

Find 32 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAACCCTATATATCAAAA+TGG 0.336251 1_1:-13805019 None:intergenic
GATACACGTTGGCTTAAATA+TGG 0.339259 1_1:+13805062 Msa0009430:three_prime_UTR
ACCTCCGCACTTGAAGTTTC+TGG 0.344839 1_1:+13804810 Msa0009430:three_prime_UTR
TGTCACAGATTCGGCATAGA+AGG 0.357548 1_1:+13804524 Msa0009430:intron
GCTCTTGTTGTCAAATATAC+AGG 0.379477 1_1:-13804548 None:intergenic
TTTGACAACAAGAGCTGAAA+AGG 0.403110 1_1:+13804556 Msa0009430:CDS
AGCTGAAGCTTTGGCCGGTT+AGG 0.424906 1_1:-13804695 None:intergenic
CTCTCATCTAGAGGTATACT+AGG 0.448622 1_1:+13804720 Msa0009430:exon
ACAAATTCACTTGGAGGTTG+TGG 0.454352 1_1:+13804664 Msa0009430:CDS
TTGTGGAGCCTCAACCTAAC+CGG 0.471178 1_1:+13804681 Msa0009430:CDS
AGCTTTGGCCGGTTAGGTTG+AGG 0.480175 1_1:-13804689 None:intergenic
ATGAGAGTAAGCTGAAGCTT+TGG 0.481186 1_1:-13804704 None:intergenic
GAGCAATATAAATAAATAAG+AGG 0.483680 1_1:-13804782 None:intergenic
CTGAAAAGGGTAGCGGAGAA+TGG 0.489319 1_1:+13804570 Msa0009430:CDS
GCTTGAGTAGTAGTGTTGCT+TGG 0.510618 1_1:+13804913 Msa0009430:three_prime_UTR
CATTGAACAAGAAGCAAGCT+AGG 0.535530 1_1:+13804752 Msa0009430:three_prime_UTR
GACGGTACAAATTCACTTGG+AGG 0.540234 1_1:+13804658 Msa0009430:CDS
GGTATTAGAATTGACTGGCA+TGG 0.541030 1_1:+13804831 Msa0009430:three_prime_UTR
TAGTTTCGTAAGTGTGAATG+AGG 0.546738 1_1:+13804990 Msa0009430:three_prime_UTR
AAACTTCAAGTGCGGAGGTG+TGG 0.547763 1_1:-13804806 None:intergenic
CAACACTACTACTCAAGCTA+TGG 0.553424 1_1:-13804908 None:intergenic
TTTCTGGTATTAGAATTGAC+TGG 0.567157 1_1:+13804826 Msa0009430:three_prime_UTR
TTGACAACAAGAGCTGAAAA+GGG 0.568719 1_1:+13804557 Msa0009430:CDS
GCAGACGGTACAAATTCACT+TGG 0.575230 1_1:+13804655 Msa0009430:CDS
AATCTCTCTCAAGTGCAAGA+TGG 0.577628 1_1:-13805088 None:intergenic
GAGTAAGCTGAAGCTTTGGC+CGG 0.579724 1_1:-13804700 None:intergenic
TTTATCAAAATGATACACGT+TGG 0.591318 1_1:+13805051 Msa0009430:three_prime_UTR
AGATGGTTACTCATTTCAGA+AGG 0.619023 1_1:+13804421 None:intergenic
ACAAGAGCTGAAAAGGGTAG+CGG 0.620766 1_1:+13804563 Msa0009430:CDS
TTCAGCTTACTCTCATCTAG+AGG 0.635613 1_1:+13804711 Msa0009430:CDS
ACCAGAAACTTCAAGTGCGG+AGG 0.640511 1_1:-13804811 None:intergenic
AATACCAGAAACTTCAAGTG+CGG 0.692524 1_1:-13804814 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAGCAATATAAATAAATAAG+AGG - chr1_1:13804785-13804804 None:intergenic 20.0%
!!! AATTGTCCATTTTGATATAT+AGG + chr1_1:13805013-13805032 Msa0009430:three_prime_UTR 20.0%
!!! ATTGTCCATTTTGATATATA+GGG + chr1_1:13805014-13805033 Msa0009430:three_prime_UTR 20.0%
!! GAGCAATATAAATAAATAAG+AGG - chr1_1:13804785-13804804 None:intergenic 20.0%
!!! AATTGTCCATTTTGATATAT+AGG + chr1_1:13805013-13805032 Msa0009430:three_prime_UTR 20.0%
!!! ATTGTCCATTTTGATATATA+GGG + chr1_1:13805014-13805033 Msa0009430:three_prime_UTR 20.0%
! ACTAACCCTATATATCAAAA+TGG - chr1_1:13805022-13805041 None:intergenic 25.0%
! TTTATCAAAATGATACACGT+TGG + chr1_1:13805051-13805070 Msa0009430:three_prime_UTR 25.0%
!!! GTATAACTGTTTTGATTAGT+TGG + chr1_1:13805112-13805131 Msa0009430:three_prime_UTR 25.0%
! ACTAACCCTATATATCAAAA+TGG - chr1_1:13805022-13805041 None:intergenic 25.0%
! TTTATCAAAATGATACACGT+TGG + chr1_1:13805051-13805070 Msa0009430:three_prime_UTR 25.0%
!!! GTATAACTGTTTTGATTAGT+TGG + chr1_1:13805112-13805131 Msa0009430:three_prime_UTR 25.0%
!!! CTTTTTTCTTGTCACAGATT+CGG + chr1_1:13804515-13804534 Msa0009430:intron 30.0%
AGAGCAGAAGATTAAAAAGA+CGG + chr1_1:13804607-13804626 Msa0009430:CDS 30.0%
! TTTCTGGTATTAGAATTGAC+TGG + chr1_1:13804826-13804845 Msa0009430:three_prime_UTR 30.0%
! ATTTTGTTGGAAGAATTGAC+TGG + chr1_1:13804856-13804875 Msa0009430:three_prime_UTR 30.0%
!!! CTTTTTTCTTGTCACAGATT+CGG + chr1_1:13804515-13804534 Msa0009430:intron 30.0%
AGAGCAGAAGATTAAAAAGA+CGG + chr1_1:13804607-13804626 Msa0009430:CDS 30.0%
! TTTCTGGTATTAGAATTGAC+TGG + chr1_1:13804826-13804845 Msa0009430:three_prime_UTR 30.0%
! ATTTTGTTGGAAGAATTGAC+TGG + chr1_1:13804856-13804875 Msa0009430:three_prime_UTR 30.0%
! GCTCTTGTTGTCAAATATAC+AGG - chr1_1:13804551-13804570 None:intergenic 35.0%
TTTGACAACAAGAGCTGAAA+AGG + chr1_1:13804556-13804575 Msa0009430:CDS 35.0%
TTGACAACAAGAGCTGAAAA+GGG + chr1_1:13804557-13804576 Msa0009430:CDS 35.0%
CAGAAGATTAAAAAGACGGA+TGG + chr1_1:13804611-13804630 Msa0009430:CDS 35.0%
! TTCATACAGCATTTTGCAGA+CGG + chr1_1:13804640-13804659 Msa0009430:CDS 35.0%
AATACCAGAAACTTCAAGTG+CGG - chr1_1:13804817-13804836 None:intergenic 35.0%
! GACTGGCATAGATATTTTGT+TGG + chr1_1:13804873-13804892 Msa0009430:three_prime_UTR 35.0%
TAGTTTCGTAAGTGTGAATG+AGG + chr1_1:13804990-13805009 Msa0009430:three_prime_UTR 35.0%
!! GATACACGTTGGCTTAAATA+TGG + chr1_1:13805062-13805081 Msa0009430:three_prime_UTR 35.0%
! GCTCTTGTTGTCAAATATAC+AGG - chr1_1:13804551-13804570 None:intergenic 35.0%
TTTGACAACAAGAGCTGAAA+AGG + chr1_1:13804556-13804575 Msa0009430:CDS 35.0%
TTGACAACAAGAGCTGAAAA+GGG + chr1_1:13804557-13804576 Msa0009430:CDS 35.0%
CAGAAGATTAAAAAGACGGA+TGG + chr1_1:13804611-13804630 Msa0009430:CDS 35.0%
! TTCATACAGCATTTTGCAGA+CGG + chr1_1:13804640-13804659 Msa0009430:CDS 35.0%
AATACCAGAAACTTCAAGTG+CGG - chr1_1:13804817-13804836 None:intergenic 35.0%
! GACTGGCATAGATATTTTGT+TGG + chr1_1:13804873-13804892 Msa0009430:three_prime_UTR 35.0%
TAGTTTCGTAAGTGTGAATG+AGG + chr1_1:13804990-13805009 Msa0009430:three_prime_UTR 35.0%
!! GATACACGTTGGCTTAAATA+TGG + chr1_1:13805062-13805081 Msa0009430:three_prime_UTR 35.0%
ACAAATTCACTTGGAGGTTG+TGG + chr1_1:13804664-13804683 Msa0009430:CDS 40.0%
ATGAGAGTAAGCTGAAGCTT+TGG - chr1_1:13804707-13804726 None:intergenic 40.0%
TTCAGCTTACTCTCATCTAG+AGG + chr1_1:13804711-13804730 Msa0009430:CDS 40.0%
! CTCTCATCTAGAGGTATACT+AGG + chr1_1:13804720-13804739 Msa0009430:exon 40.0%
CATTGAACAAGAAGCAAGCT+AGG + chr1_1:13804752-13804771 Msa0009430:three_prime_UTR 40.0%
GGTATTAGAATTGACTGGCA+TGG + chr1_1:13804831-13804850 Msa0009430:three_prime_UTR 40.0%
! GACTGGCATGGATATTTTGT+TGG + chr1_1:13804843-13804862 Msa0009430:three_prime_UTR 40.0%
CAACACTACTACTCAAGCTA+TGG - chr1_1:13804911-13804930 None:intergenic 40.0%
AATCTCTCTCAAGTGCAAGA+TGG - chr1_1:13805091-13805110 None:intergenic 40.0%
ACAAATTCACTTGGAGGTTG+TGG + chr1_1:13804664-13804683 Msa0009430:CDS 40.0%
ATGAGAGTAAGCTGAAGCTT+TGG - chr1_1:13804707-13804726 None:intergenic 40.0%
TTCAGCTTACTCTCATCTAG+AGG + chr1_1:13804711-13804730 Msa0009430:CDS 40.0%
! CTCTCATCTAGAGGTATACT+AGG + chr1_1:13804720-13804739 Msa0009430:exon 40.0%
CATTGAACAAGAAGCAAGCT+AGG + chr1_1:13804752-13804771 Msa0009430:three_prime_UTR 40.0%
GGTATTAGAATTGACTGGCA+TGG + chr1_1:13804831-13804850 Msa0009430:three_prime_UTR 40.0%
! GACTGGCATGGATATTTTGT+TGG + chr1_1:13804843-13804862 Msa0009430:three_prime_UTR 40.0%
CAACACTACTACTCAAGCTA+TGG - chr1_1:13804911-13804930 None:intergenic 40.0%
AATCTCTCTCAAGTGCAAGA+TGG - chr1_1:13805091-13805110 None:intergenic 40.0%
TGTCACAGATTCGGCATAGA+AGG + chr1_1:13804524-13804543 Msa0009430:intron 45.0%
ACAAGAGCTGAAAAGGGTAG+CGG + chr1_1:13804563-13804582 Msa0009430:CDS 45.0%
GCAGACGGTACAAATTCACT+TGG + chr1_1:13804655-13804674 Msa0009430:CDS 45.0%
GACGGTACAAATTCACTTGG+AGG + chr1_1:13804658-13804677 Msa0009430:CDS 45.0%
! GCTTGAGTAGTAGTGTTGCT+TGG + chr1_1:13804913-13804932 Msa0009430:three_prime_UTR 45.0%
TGTCACAGATTCGGCATAGA+AGG + chr1_1:13804524-13804543 Msa0009430:intron 45.0%
ACAAGAGCTGAAAAGGGTAG+CGG + chr1_1:13804563-13804582 Msa0009430:CDS 45.0%
GCAGACGGTACAAATTCACT+TGG + chr1_1:13804655-13804674 Msa0009430:CDS 45.0%
GACGGTACAAATTCACTTGG+AGG + chr1_1:13804658-13804677 Msa0009430:CDS 45.0%
! GCTTGAGTAGTAGTGTTGCT+TGG + chr1_1:13804913-13804932 Msa0009430:three_prime_UTR 45.0%
!! CTGAAAAGGGTAGCGGAGAA+TGG + chr1_1:13804570-13804589 Msa0009430:CDS 50.0%
TTGTGGAGCCTCAACCTAAC+CGG + chr1_1:13804681-13804700 Msa0009430:CDS 50.0%
! GAGTAAGCTGAAGCTTTGGC+CGG - chr1_1:13804703-13804722 None:intergenic 50.0%
AAACTTCAAGTGCGGAGGTG+TGG - chr1_1:13804809-13804828 None:intergenic 50.0%
ACCTCCGCACTTGAAGTTTC+TGG + chr1_1:13804810-13804829 Msa0009430:three_prime_UTR 50.0%
ACCAGAAACTTCAAGTGCGG+AGG - chr1_1:13804814-13804833 None:intergenic 50.0%
!! CTGAAAAGGGTAGCGGAGAA+TGG + chr1_1:13804570-13804589 Msa0009430:CDS 50.0%
TTGTGGAGCCTCAACCTAAC+CGG + chr1_1:13804681-13804700 Msa0009430:CDS 50.0%
! GAGTAAGCTGAAGCTTTGGC+CGG - chr1_1:13804703-13804722 None:intergenic 50.0%
AAACTTCAAGTGCGGAGGTG+TGG - chr1_1:13804809-13804828 None:intergenic 50.0%
ACCTCCGCACTTGAAGTTTC+TGG + chr1_1:13804810-13804829 Msa0009430:three_prime_UTR 50.0%
ACCAGAAACTTCAAGTGCGG+AGG - chr1_1:13804814-13804833 None:intergenic 50.0%
! AGCTTTGGCCGGTTAGGTTG+AGG - chr1_1:13804692-13804711 None:intergenic 55.0%
! AGCTGAAGCTTTGGCCGGTT+AGG - chr1_1:13804698-13804717 None:intergenic 55.0%
! AGCTTTGGCCGGTTAGGTTG+AGG - chr1_1:13804692-13804711 None:intergenic 55.0%
! AGCTGAAGCTTTGGCCGGTT+AGG - chr1_1:13804698-13804717 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 13804423 13805179 13804423 ID=Msa0009430;Name=Msa0009430
chr1_1 mRNA 13804423 13805179 13804423 ID=Msa0009430-mRNA-1;Parent=Msa0009430;Name=Msa0009430-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0.5|0.5|1|1|2|449|72
chr1_1 exon 13804423 13804442 13804423 ID=Msa0009430-mRNA-1:exon:5236;Parent=Msa0009430-mRNA-1
chr1_1 exon 13804532 13805179 13804532 ID=Msa0009430-mRNA-1:exon:5237;Parent=Msa0009430-mRNA-1
chr1_1 CDS 13804423 13804442 13804423 ID=Msa0009430-mRNA-1:cds;Parent=Msa0009430-mRNA-1
chr1_1 CDS 13804532 13804730 13804532 ID=Msa0009430-mRNA-1:cds;Parent=Msa0009430-mRNA-1
chr1_1 three_prime_UTR 13804731 13805179 13804731 ID=Msa0009430-mRNA-1:three_prime_utr;Parent=Msa0009430-mRNA-1
Gene Sequence

>Msa0009430

ATGGTTACTCATTTCAGAAGATTCGGCATAGAAGGACCTGTATATTTGACAACAAGAGCTGAAAAGGGTAGCGGAGAATGGTATGTAGATGAACAAGAGCAGAAGATTAAAAAGACGGATGGTAGCTTTTCATACAGCATTTTGCAGACGGTACAAATTCACTTGGAGGTTGTGGAGCCTCAACCTAACCGGCCAAAGCTTCAGCTTACTCTCATCTAG

Protein sequence

>Msa0009430

MVTHFRRFGIEGPVYLTTRAEKGSGEWYVDEQEQKIKKTDGSFSYSILQTVQIHLEVVEPQPNRPKLQLTLI*