AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0383690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383690 MtrunA17_Chr3g0140281 94.624 93 2 1 1 90 44 136 8.56e-55 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383690 AT5G11630.1 60.215 93 34 1 1 90 1 93 1.12e-28 99.4
Msa0383690 AT5G11630.3 60.215 93 34 1 1 90 1 93 1.12e-28 99.4
Msa0383690 AT5G11630.2 54.118 85 36 1 1 82 1 85 6.36e-20 77.0
Msa0383690 AT4G17310.11 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT4G17310.13 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT4G17310.1 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT4G17310.6 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT4G17310.12 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT4G17310.9 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT4G17310.5 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT4G17310.17 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT4G17310.4 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT4G17310.3 69.565 46 14 0 45 90 54 99 3.64e-15 65.1
Msa0383690 AT5G47455.10 64.000 50 18 0 41 90 59 108 1.58e-14 63.5
Msa0383690 AT5G47455.8 64.000 50 18 0 41 90 59 108 1.58e-14 63.5
Msa0383690 AT5G47455.9 64.000 50 18 0 41 90 59 108 1.58e-14 63.5
Msa0383690 AT4G17310.16 78.378 37 8 0 45 81 54 90 9.94e-13 58.5
Msa0383690 AT4G17310.7 78.378 37 8 0 45 81 54 90 9.94e-13 58.5
Msa0383690 AT4G17310.14 78.378 37 8 0 45 81 54 90 9.94e-13 58.5
Msa0383690 AT4G17310.8 78.378 37 8 0 45 81 54 90 9.94e-13 58.5
Msa0383690 AT4G17310.10 78.378 37 8 0 45 81 54 90 9.94e-13 58.5
Msa0383690 AT5G47455.1 75.676 37 9 0 45 81 63 99 3.06e-12 57.8
Msa0383690 AT5G47455.11 75.676 37 9 0 45 81 63 99 3.06e-12 57.8
Msa0383690 AT5G47455.7 75.676 37 9 0 45 81 63 99 3.06e-12 57.8
Msa0383690 AT5G47455.6 75.676 37 9 0 45 81 63 99 3.06e-12 57.8
Msa0383690 AT5G47455.3 73.684 38 10 0 45 82 63 100 8.15e-12 56.6
Msa0383690 AT4G17310.2 73.684 38 10 0 45 82 54 91 1.01e-11 56.2
Msa0383690 AT4G17310.15 77.778 36 8 0 45 80 54 89 1.53e-11 55.8
Msa0383690 AT5G47455.4 75.000 36 9 0 45 80 63 98 2.92e-11 55.5
Msa0383690 AT5G47455.5 75.000 36 9 0 45 80 63 98 4.21e-11 54.7
Msa0383690 AT5G47455.2 75.000 36 9 0 45 80 63 98 4.21e-11 54.7

Find 53 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCTAGGTGTGCGATAATT+TGG 0.137178 3_1:+86680667 Msa0383690:five_prime_UTR
AAAGGGTGAGAAGGAGGATT+TGG 0.218439 3_1:-86680781 None:intergenic
CATCATAATGGGTATATAAT+TGG 0.259213 3_1:+86683740 Msa0383690:three_prime_UTR
CCCTAGGTGTGCGATAATTT+GGG 0.303269 3_1:+86680668 Msa0383690:five_prime_UTR
AAGGGTGAGAAGGAGGATTT+GGG 0.332675 3_1:-86680780 None:intergenic
TCTTTGACAGACCATCATAA+TGG 0.348227 3_1:+86683728 Msa0383690:three_prime_UTR
CTTTGACAGACCATCATAAT+GGG 0.380291 3_1:+86683729 Msa0383690:three_prime_UTR
GGATTTGGGTTTGGTGATGG+TGG 0.389511 3_1:-86680766 None:intergenic
TTTGAAAGGCTGGTGATTGA+TGG 0.392484 3_1:+86683654 Msa0383690:three_prime_UTR
TGAGAAGGAGGATTTGGGTT+TGG 0.407768 3_1:-86680775 None:intergenic
GGCGCCGATTTGTTCTTCAA+TGG 0.409996 3_1:+86680695 Msa0383690:exon
CAAGTTTCTGAAAAGCAGAT+TGG 0.413390 3_1:+86683882 Msa0383690:three_prime_UTR
AAGTTCGAGATCTTTGTCTC+AGG 0.419533 3_1:+86681009 Msa0383690:CDS
GAGGAGAAAGGGTGAGAAGG+AGG 0.439652 3_1:-86680787 None:intergenic
ATTTCCTCAGAAATGGGTCT+TGG 0.443101 3_1:+86683614 Msa0383690:intron
CTCGCACTTCCGTCACCGTC+AGG 0.447447 3_1:+86680814 Msa0383690:CDS
GGAGGATTTGGGTTTGGTGA+TGG 0.450114 3_1:-86680769 None:intergenic
TACAGGTCTGTCGTGGAGCT+TGG 0.450797 3_1:+86680908 Msa0383690:intron
ACTAGAGTCATTGCTTTGTC+AGG 0.456305 3_1:+86683677 Msa0383690:three_prime_UTR
TACTACACAATAGATACAAA+AGG 0.466403 3_1:-86680637 None:intergenic
CAATTATATACCCATTATGA+TGG 0.474592 3_1:-86683739 None:intergenic
ACAATTAACAATGAAATGAT+CGG 0.497664 3_1:-86683802 None:intergenic
TTTGAGGAGAAAGGGTGAGA+AGG 0.497764 3_1:-86680790 None:intergenic
AGTAAGACGCGCTGATGAAA+CGG 0.499949 3_1:-86680963 None:intergenic
CAATTAACAATGAAATGATC+GGG 0.504058 3_1:-86683801 None:intergenic
ATTACACTGTAGCTAGAAGA+TGG 0.508920 3_1:-86683773 None:intergenic
ACTACACAATAGATACAAAA+GGG 0.517217 3_1:-86680636 None:intergenic
TGATGAAACGGCCGAGTGCA+AGG 0.520941 3_1:-86680951 None:intergenic
GCGCGTCTTACTTCTTGCCT+CGG 0.528907 3_1:+86680974 Msa0383690:CDS
CCCAAATTATCGCACACCTA+GGG 0.532576 3_1:-86680668 None:intergenic
GTCACTCCTTCCCTTGCACT+CGG 0.535547 3_1:+86680940 Msa0383690:CDS
AGTTCGAGATCTTTGTCTCA+GGG 0.539010 3_1:+86681010 Msa0383690:CDS
CGATAACAGATTTGAAAGGC+TGG 0.539663 3_1:+86683644 Msa0383690:three_prime_UTR
GATTTGATTAGAGAGAATGC+TGG 0.550233 3_1:-86680744 None:intergenic
ATGCTTTGCAGTGCCAACCC+TGG 0.558470 3_1:+86681034 Msa0383690:CDS
GAAGCCATTGAAGAACAAAT+CGG 0.564656 3_1:-86680699 None:intergenic
AATGAATCAAACACCAGGGT+TGG 0.580607 3_1:-86681047 None:intergenic
AATATGCTTACGGATAGAAA+AGG 0.586691 3_1:-86681075 None:intergenic
GATGAAACGGCCGAGTGCAA+GGG 0.589191 3_1:-86680950 None:intergenic
AGATAAATGAATCAAACACC+AGG 0.590847 3_1:-86681052 None:intergenic
GCCTTTCAAATCTGTTATCG+CGG 0.591229 3_1:-86683641 None:intergenic
CCTAGGTGTGCGATAATTTG+GGG 0.597260 3_1:+86680669 Msa0383690:five_prime_UTR
GTGTAGTAAGTATAAACCCT+AGG 0.601862 3_1:+86680652 Msa0383690:five_prime_UTR
CCCCAAATTATCGCACACCT+AGG 0.602015 3_1:-86680669 None:intergenic
TCCACCGTACAGGTCTGTCG+TGG 0.613492 3_1:+86680901 Msa0383690:intron
AACTTGTTGAATCGATTCCG+AGG 0.629649 3_1:-86680991 None:intergenic
AAACGGCCGAGTGCAAGGGA+AGG 0.634854 3_1:-86680946 None:intergenic
GATGAGTTACCTGACGGTGA+CGG 0.642581 3_1:-86680823 None:intergenic
ACCGCGATAACAGATTTGAA+AGG 0.645182 3_1:+86683640 Msa0383690:three_prime_UTR
CTAGGTGTGCGATAATTTGG+GGG 0.672527 3_1:+86680670 Msa0383690:five_prime_UTR
GATAAATGAATCAAACACCA+GGG 0.682815 3_1:-86681051 None:intergenic
ATTCTGGGAAAATCGTAGCG+AGG 0.708626 3_1:-86683916 None:intergenic
TAGGTGTGCGATAATTTGGG+GGG 0.717295 3_1:+86680671 Msa0383690:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGCATTTTAATTTATTT+TGG + chr3_1:86681161-86681180 Msa0383690:intron 10.0%
!!! TTTATATTTTTACCTAAATA+TGG + chr3_1:86682303-86682322 Msa0383690:intron 10.0%
!!! TCAAATTATTCAAAATTTTA+GGG - chr3_1:86682522-86682541 None:intergenic 10.0%
!!! ATCAAATTATTCAAAATTTT+AGG - chr3_1:86682523-86682542 None:intergenic 10.0%
!! GTTTATGTTATTAAAAATAG+AGG + chr3_1:86681609-86681628 Msa0383690:intron 15.0%
!!! ATGTTTGTTTTTTTTTATCA+TGG + chr3_1:86683040-86683059 Msa0383690:intron 15.0%
!!! TGTTTGTTTTTTTTTATCAT+GGG + chr3_1:86683041-86683060 Msa0383690:intron 15.0%
!! TACATTAAAATGGAAGAAAA+AGG - chr3_1:86680857-86680876 None:intergenic 20.0%
!! GAATTAACGTTACATTAAAA+TGG - chr3_1:86680867-86680886 None:intergenic 20.0%
!!! TTTTAATCACCAATAAACAA+TGG - chr3_1:86681266-86681285 None:intergenic 20.0%
!!! TGTTTATTGGTGATTAAAAA+AGG + chr3_1:86681267-86681286 Msa0383690:intron 20.0%
!!! GTTTATTGGTGATTAAAAAA+GGG + chr3_1:86681268-86681287 Msa0383690:intron 20.0%
!! ATTAAAAATAGAGGTCATAA+TGG + chr3_1:86681618-86681637 Msa0383690:intron 20.0%
!! AAATTCTGTTATAAATATGC+TGG + chr3_1:86681698-86681717 Msa0383690:intron 20.0%
!! CAAGTTACATAAACAAAAAA+TGG - chr3_1:86681850-86681869 None:intergenic 20.0%
!!! CATTTTTTGTTTATGTAACT+TGG + chr3_1:86681848-86681867 Msa0383690:intron 20.0%
!!! TGAATGTCTTTCATTTTTTA+AGG + chr3_1:86682011-86682030 Msa0383690:intron 20.0%
!! ATTCTAAATTCTCCATATTT+AGG - chr3_1:86682318-86682337 None:intergenic 20.0%
!! AGGCTATCTAAATAATAAAT+AGG - chr3_1:86682460-86682479 None:intergenic 20.0%
!!! ATTCCTAAAGCTTTTTTTTA+GGG + chr3_1:86682489-86682508 Msa0383690:intron 20.0%
!! AAAAATACTGAAATGATCTA+TGG + chr3_1:86682727-86682746 Msa0383690:intron 20.0%
!!! GCATCTATTTTCAAATTTAA+TGG - chr3_1:86682860-86682879 None:intergenic 20.0%
!! TTTACAACTCTTACATATAT+AGG - chr3_1:86682955-86682974 None:intergenic 20.0%
!! GTTTACTTTGTGTATTATTA+TGG + chr3_1:86683086-86683105 Msa0383690:intron 20.0%
!! TTAACATAGACAATAATACA+TGG - chr3_1:86683556-86683575 None:intergenic 20.0%
!! AAAAAGATAAACATTAAGCA+AGG - chr3_1:86683591-86683610 None:intergenic 20.0%
!!! TTTTAAAATTTCCTCAGAAA+TGG + chr3_1:86683607-86683626 Msa0383690:intron 20.0%
!!! TTTAAAATTTCCTCAGAAAT+GGG + chr3_1:86683608-86683627 Msa0383690:intron 20.0%
!! ACAATTAACAATGAAATGAT+CGG - chr3_1:86683805-86683824 None:intergenic 20.0%
! ATTAAAATGGAAGAAAAAGG+TGG - chr3_1:86680854-86680873 None:intergenic 25.0%
! ATGAATTATGCATTAATGAG+TGG - chr3_1:86681109-86681128 None:intergenic 25.0%
!! TAATTATGTTTCTGTACTCA+TGG + chr3_1:86681233-86681252 Msa0383690:intron 25.0%
! TAGGATTTAATACACTTTCA+GGG - chr3_1:86681302-86681321 None:intergenic 25.0%
! TTAGGATTTAATACACTTTC+AGG - chr3_1:86681303-86681322 None:intergenic 25.0%
!! ATGCAGTATACATTTTTGTA+AGG + chr3_1:86681390-86681409 Msa0383690:intron 25.0%
!! TTTTTGTAAGGTTACAGTTT+AGG + chr3_1:86681402-86681421 Msa0383690:intron 25.0%
!! TCCTTCTAATTCTGTAATTT+TGG + chr3_1:86681913-86681932 Msa0383690:intron 25.0%
!! CCTTCTAATTCTGTAATTTT+GGG + chr3_1:86681914-86681933 Msa0383690:intron 25.0%
! TTGTAGTTCAGGAAAAAAAA+TGG - chr3_1:86682082-86682101 None:intergenic 25.0%
! AATAACCTTTATCTACTTAC+AGG - chr3_1:86682267-86682286 None:intergenic 25.0%
!!! ATGTTAAGGATTTTAAGGAA+AGG + chr3_1:86682417-86682436 Msa0383690:intron 25.0%
!!! GATTCCTAAAGCTTTTTTTT+AGG + chr3_1:86682488-86682507 Msa0383690:intron 25.0%
!!! TTCCTAAAGCTTTTTTTTAG+GGG + chr3_1:86682490-86682509 Msa0383690:intron 25.0%
! AATTACGCTAATTTGATGAA+TGG + chr3_1:86682561-86682580 Msa0383690:intron 25.0%
! TCTTTGATAAAAGTAATCGT+TGG - chr3_1:86682596-86682615 None:intergenic 25.0%
!!! TACTGTTTTTTAGACATCTA+TGG + chr3_1:86682908-86682927 Msa0383690:intron 25.0%
!!! AACTGTTTTTTCAATCTATG+TGG + chr3_1:86683164-86683183 Msa0383690:intron 25.0%
!!! TTTTTTCAATCTATGTGGAT+AGG + chr3_1:86683169-86683188 Msa0383690:intron 25.0%
!!! TTTTTCAATCTATGTGGATA+GGG + chr3_1:86683170-86683189 Msa0383690:intron 25.0%
! GCACTAATATTATATACTCA+GGG - chr3_1:86683226-86683245 None:intergenic 25.0%
! TGCACTAATATTATATACTC+AGG - chr3_1:86683227-86683246 None:intergenic 25.0%
! ATTCATTTGTTTCATCTAAG+TGG + chr3_1:86683309-86683328 Msa0383690:intron 25.0%
! GATTATATATAGTCACAACA+TGG - chr3_1:86683496-86683515 None:intergenic 25.0%
! CAATTATATACCCATTATGA+TGG - chr3_1:86683742-86683761 None:intergenic 25.0%
!!! CATCATAATGGGTATATAAT+TGG + chr3_1:86683740-86683759 Msa0383690:three_prime_UTR 25.0%
! CAATTAACAATGAAATGATC+GGG - chr3_1:86683804-86683823 None:intergenic 25.0%
GATAAATGAATCAAACACCA+GGG - chr3_1:86681054-86681073 None:intergenic 30.0%
AGATAAATGAATCAAACACC+AGG - chr3_1:86681055-86681074 None:intergenic 30.0%
AATATGCTTACGGATAGAAA+AGG - chr3_1:86681078-86681097 None:intergenic 30.0%
TTAGCTATATTTGTGCGATT+AGG + chr3_1:86681189-86681208 Msa0383690:intron 30.0%
TTGACTAACAGACTAAATCA+TGG - chr3_1:86681448-86681467 None:intergenic 30.0%
TAGTCAAAAGAGAGTAAAGA+TGG + chr3_1:86681461-86681480 Msa0383690:intron 30.0%
GAAATTTGTATTGAGAAGAC+AGG + chr3_1:86681518-86681537 Msa0383690:intron 30.0%
TTCTGTTATAAATATGCTGG+TGG + chr3_1:86681701-86681720 Msa0383690:intron 30.0%
!! GAGTGTATTATTTCTGCAAT+AGG - chr3_1:86681798-86681817 None:intergenic 30.0%
CCCAAAATTACAGAATTAGA+AGG - chr3_1:86681917-86681936 None:intergenic 30.0%
GCACTAATTCATTAGGATTT+AGG - chr3_1:86681975-86681994 None:intergenic 30.0%
TAATAGAGTTCTTGTAGTTC+AGG - chr3_1:86682093-86682112 None:intergenic 30.0%
TAGAATACGAATGTCAAACA+AGG + chr3_1:86682332-86682351 Msa0383690:intron 30.0%
AGAATACGAATGTCAAACAA+GGG + chr3_1:86682333-86682352 Msa0383690:intron 30.0%
! GAGCAATGTTAAGGATTTTA+AGG + chr3_1:86682412-86682431 Msa0383690:intron 30.0%
ATCATGTTCATATGAAACCT+CGG - chr3_1:86682821-86682840 None:intergenic 30.0%
CAAATTTAATGGTACCATCT+AGG - chr3_1:86682849-86682868 None:intergenic 30.0%
GTATGAGTAGTCTGTATTAT+AGG + chr3_1:86682982-86683001 Msa0383690:intron 30.0%
! TTTTCAATCTATGTGGATAG+GGG + chr3_1:86683171-86683190 Msa0383690:intron 30.0%
!!! TCTGTCTTTTGGTTTTTAGT+AGG - chr3_1:86683367-86683386 None:intergenic 30.0%
! AAATGTAGTGTTCTGTCTTT+TGG - chr3_1:86683378-86683397 None:intergenic 30.0%
GTGTAGTAAGTATAAACCCT+AGG + chr3_1:86680652-86680671 Msa0383690:five_prime_UTR 35.0%
GAAGCCATTGAAGAACAAAT+CGG - chr3_1:86680702-86680721 None:intergenic 35.0%
GATTTGATTAGAGAGAATGC+TGG - chr3_1:86680747-86680766 None:intergenic 35.0%
GGAAAGTTGCAATATGCTTA+CGG - chr3_1:86681088-86681107 None:intergenic 35.0%
GGATGACATCCATTGTTTAT+TGG + chr3_1:86681254-86681273 Msa0383690:intron 35.0%
TAAGGTTACAGTTTAGGTCA+GGG + chr3_1:86681408-86681427 Msa0383690:intron 35.0%
TTGAACTGACCTTCTCATTT+GGG - chr3_1:86681545-86681564 None:intergenic 35.0%
TTTGAACTGACCTTCTCATT+TGG - chr3_1:86681546-86681565 None:intergenic 35.0%
AAATGAGAAGGTCAGTTCAA+AGG + chr3_1:86681545-86681564 Msa0383690:intron 35.0%
AATGAGAAGGTCAGTTCAAA+GGG + chr3_1:86681546-86681565 Msa0383690:intron 35.0%
ACATAAACTCTCCACACTAT+GGG - chr3_1:86681597-86681616 None:intergenic 35.0%
AACATAAACTCTCCACACTA+TGG - chr3_1:86681598-86681617 None:intergenic 35.0%
TCATAATGGCAATATCCACA+TGG + chr3_1:86681632-86681651 Msa0383690:intron 35.0%
TGAGAGTGAAAGGATAGAAA+CGG + chr3_1:86681656-86681675 Msa0383690:intron 35.0%
GCTTTGCATGAGAAATTGAA+TGG + chr3_1:86681870-86681889 Msa0383690:intron 35.0%
! ACACAGAGCACTAATTCATT+AGG - chr3_1:86681982-86682001 None:intergenic 35.0%
!!! CATTTTTTAAGGCCTTGCAT+AGG + chr3_1:86682022-86682041 Msa0383690:intron 35.0%
CTAGACCTGTAAGTAGATAA+AGG + chr3_1:86682259-86682278 Msa0383690:intron 35.0%
ATAGATTGTGAAACGTAGAG+TGG + chr3_1:86682382-86682401 Msa0383690:intron 35.0%
TGCCCCTAAAAAAAAGCTTT+AGG - chr3_1:86682495-86682514 None:intergenic 35.0%
TAACCAAAAAGCAATAACCG+AGG + chr3_1:86682801-86682820 Msa0383690:intron 35.0%
!! AAACCTCGGTTATTGCTTTT+TGG - chr3_1:86682807-86682826 None:intergenic 35.0%
GAACATGATATGTACCTAGA+TGG + chr3_1:86682832-86682851 Msa0383690:intron 35.0%
AGAACATACGAAGAATGGAT+AGG - chr3_1:86683257-86683276 None:intergenic 35.0%
CTAGAAGAACATACGAAGAA+TGG - chr3_1:86683262-86683281 None:intergenic 35.0%
GTGGTTAAGCAAATGATATG+TGG + chr3_1:86683328-86683347 Msa0383690:intron 35.0%
TCTTTGACAGACCATCATAA+TGG + chr3_1:86683728-86683747 Msa0383690:three_prime_UTR 35.0%
CTTTGACAGACCATCATAAT+GGG + chr3_1:86683729-86683748 Msa0383690:three_prime_UTR 35.0%
ATTACACTGTAGCTAGAAGA+TGG - chr3_1:86683776-86683795 None:intergenic 35.0%
! CAAGTTTCTGAAAAGCAGAT+TGG + chr3_1:86683882-86683901 Msa0383690:three_prime_UTR 35.0%
ATGGAAGAAAAAGGTGGAAG+AGG - chr3_1:86680848-86680867 None:intergenic 40.0%
!! AACTTGTTGAATCGATTCCG+AGG - chr3_1:86680994-86681013 None:intergenic 40.0%
AAGTTCGAGATCTTTGTCTC+AGG + chr3_1:86681009-86681028 Msa0383690:CDS 40.0%
AGTTCGAGATCTTTGTCTCA+GGG + chr3_1:86681010-86681029 Msa0383690:CDS 40.0%
AATGAATCAAACACCAGGGT+TGG - chr3_1:86681050-86681069 None:intergenic 40.0%
!! GCTCAAAGCGTCATTTTGTT+AGG - chr3_1:86681321-86681340 None:intergenic 40.0%
GTAAGGTTACAGTTTAGGTC+AGG + chr3_1:86681407-86681426 Msa0383690:intron 40.0%
AAGGTTACAGTTTAGGTCAG+GGG + chr3_1:86681409-86681428 Msa0383690:intron 40.0%
ATGAGAAGGTCAGTTCAAAG+GGG + chr3_1:86681547-86681566 Msa0383690:intron 40.0%
GAGAGTGAAAGGATAGAAAC+GGG + chr3_1:86681657-86681676 Msa0383690:intron 40.0%
TGAAAGGATAGAAACGGGAT+AGG + chr3_1:86681662-86681681 Msa0383690:intron 40.0%
!! GTGGAAAGAGAAAAGCATCA+AGG + chr3_1:86681720-86681739 Msa0383690:intron 40.0%
GAATCATACAGTTTGTCAGG+TGG + chr3_1:86681744-86681763 Msa0383690:intron 40.0%
TGGTTTCTTCTGTGTCGTAT+AGG + chr3_1:86681890-86681909 Msa0383690:intron 40.0%
TAAGGCCTTGCATAGGAAAT+AGG + chr3_1:86682029-86682048 Msa0383690:intron 40.0%
AAGGCCTTGCATAGGAAATA+GGG + chr3_1:86682030-86682049 Msa0383690:intron 40.0%
TCTTCCCTATTTCCTATGCA+AGG - chr3_1:86682037-86682056 None:intergenic 40.0%
GGAAAAGCAGAGCAATGTTA+AGG + chr3_1:86682403-86682422 Msa0383690:intron 40.0%
!! GCTTTAGGAATCGTCTACTA+AGG - chr3_1:86682480-86682499 None:intergenic 40.0%
!! GATCTATGGCTTGTTCTGAT+TGG + chr3_1:86682741-86682760 Msa0383690:intron 40.0%
!!! TGCTTTTTGGTTAAGCTCGA+AGG - chr3_1:86682794-86682813 None:intergenic 40.0%
TAGATGCAATAGACCACTTG+TGG + chr3_1:86682873-86682892 Msa0383690:intron 40.0%
AGTTTATTACGCTCCACAAG+TGG - chr3_1:86682889-86682908 None:intergenic 40.0%
ATTAAGCAAGGCACATGACA+TGG - chr3_1:86683579-86683598 None:intergenic 40.0%
ATTTCCTCAGAAATGGGTCT+TGG + chr3_1:86683614-86683633 Msa0383690:intron 40.0%
ACCGCGATAACAGATTTGAA+AGG + chr3_1:86683640-86683659 Msa0383690:three_prime_UTR 40.0%
GCCTTTCAAATCTGTTATCG+CGG - chr3_1:86683644-86683663 None:intergenic 40.0%
CGATAACAGATTTGAAAGGC+TGG + chr3_1:86683644-86683663 Msa0383690:three_prime_UTR 40.0%
TTTGAAAGGCTGGTGATTGA+TGG + chr3_1:86683654-86683673 Msa0383690:three_prime_UTR 40.0%
ACTAGAGTCATTGCTTTGTC+AGG + chr3_1:86683677-86683696 Msa0383690:three_prime_UTR 40.0%
ACCCTAGGTGTGCGATAATT+TGG + chr3_1:86680667-86680686 Msa0383690:five_prime_UTR 45.0%
CCCAAATTATCGCACACCTA+GGG - chr3_1:86680671-86680690 None:intergenic 45.0%
CCCTAGGTGTGCGATAATTT+GGG + chr3_1:86680668-86680687 Msa0383690:five_prime_UTR 45.0%
CCTAGGTGTGCGATAATTTG+GGG + chr3_1:86680669-86680688 Msa0383690:five_prime_UTR 45.0%
CTAGGTGTGCGATAATTTGG+GGG + chr3_1:86680670-86680689 Msa0383690:five_prime_UTR 45.0%
TAGGTGTGCGATAATTTGGG+GGG + chr3_1:86680671-86680690 Msa0383690:five_prime_UTR 45.0%
! TGAGAAGGAGGATTTGGGTT+TGG - chr3_1:86680778-86680797 None:intergenic 45.0%
AAGGGTGAGAAGGAGGATTT+GGG - chr3_1:86680783-86680802 None:intergenic 45.0%
AAAGGGTGAGAAGGAGGATT+TGG - chr3_1:86680784-86680803 None:intergenic 45.0%
TTTGAGGAGAAAGGGTGAGA+AGG - chr3_1:86680793-86680812 None:intergenic 45.0%
!!! GTGCGAGTTTTGAGGAGAAA+GGG - chr3_1:86680801-86680820 None:intergenic 45.0%
!!! AGTGCGAGTTTTGAGGAGAA+AGG - chr3_1:86680802-86680821 None:intergenic 45.0%
GAAGAGGATGAGTTACCTGA+CGG - chr3_1:86680832-86680851 None:intergenic 45.0%
! AGTAAGACGCGCTGATGAAA+CGG - chr3_1:86680966-86680985 None:intergenic 45.0%
TTACAGTTTAGGTCAGGGGT+GGG + chr3_1:86681413-86681432 Msa0383690:intron 45.0%
AAGACAGGACCCAAATGAGA+AGG + chr3_1:86681533-86681552 Msa0383690:intron 45.0%
AACTCTCCACACTATGGGTT+CGG - chr3_1:86681592-86681611 None:intergenic 45.0%
GGCGAATCATACAGTTTGTC+AGG + chr3_1:86681741-86681760 Msa0383690:intron 45.0%
TGAAGTCGCAGTGAATTGAG+CGG - chr3_1:86683410-86683429 None:intergenic 45.0%
ATTCACTGCGACTTCATGCT+TGG + chr3_1:86683414-86683433 Msa0383690:intron 45.0%
CCCCAAATTATCGCACACCT+AGG - chr3_1:86680672-86680691 None:intergenic 50.0%
!! GGCGCCGATTTGTTCTTCAA+TGG + chr3_1:86680695-86680714 Msa0383690:exon 50.0%
!! GGATTTGGGTTTGGTGATGG+TGG - chr3_1:86680769-86680788 None:intergenic 50.0%
!! GGAGGATTTGGGTTTGGTGA+TGG - chr3_1:86680772-86680791 None:intergenic 50.0%
!! TGACGGAAGTGCGAGTTTTG+AGG - chr3_1:86680809-86680828 None:intergenic 50.0%
GATGAGTTACCTGACGGTGA+CGG - chr3_1:86680826-86680845 None:intergenic 50.0%
TCTTCTCTTCTCCACCGTAC+AGG + chr3_1:86680891-86680910 Msa0383690:intron 50.0%
GTTACAGTTTAGGTCAGGGG+TGG + chr3_1:86681412-86681431 Msa0383690:intron 50.0%
CCAGTAATTCACACACGCAG+GGG - chr3_1:86681491-86681510 None:intergenic 50.0%
GCCAGTAATTCACACACGCA+GGG - chr3_1:86681492-86681511 None:intergenic 50.0%
TGCCAGTAATTCACACACGC+AGG - chr3_1:86681493-86681512 None:intergenic 50.0%
ACTCTCCACACTATGGGTTC+GGG - chr3_1:86681591-86681610 None:intergenic 50.0%
TCCACATGGCTGAGAGTGAA+AGG + chr3_1:86681646-86681665 Msa0383690:intron 50.0%
TCCTTTCACTCTCAGCCATG+TGG - chr3_1:86681650-86681669 None:intergenic 50.0%
! ACGGGATAGGCATCGTGTAA+CGG + chr3_1:86681675-86681694 Msa0383690:intron 50.0%
TGGAGAGAGCTGTGGACAAT+AGG + chr3_1:86681764-86681783 Msa0383690:intron 50.0%
GAAGTCGCAGTGAATTGAGC+GGG - chr3_1:86683409-86683428 None:intergenic 50.0%
TGTGCCAAGACCCATTTCTG+AGG - chr3_1:86683621-86683640 None:intergenic 50.0%
GTGTGCGATAATTTGGGGGG+TGG + chr3_1:86680674-86680693 Msa0383690:five_prime_UTR 55.0%
GAGGAGAAAGGGTGAGAAGG+AGG - chr3_1:86680790-86680809 None:intergenic 55.0%
AGCTCCACGACAGACCTGTA+CGG - chr3_1:86680908-86680927 None:intergenic 55.0%
TACAGGTCTGTCGTGGAGCT+TGG + chr3_1:86680908-86680927 Msa0383690:intron 55.0%
GTCACTCCTTCCCTTGCACT+CGG + chr3_1:86680940-86680959 Msa0383690:CDS 55.0%
GATGAAACGGCCGAGTGCAA+GGG - chr3_1:86680953-86680972 None:intergenic 55.0%
TGATGAAACGGCCGAGTGCA+AGG - chr3_1:86680954-86680973 None:intergenic 55.0%
GCGCGTCTTACTTCTTGCCT+CGG + chr3_1:86680974-86680993 Msa0383690:CDS 55.0%
ATGCTTTGCAGTGCCAACCC+TGG + chr3_1:86681034-86681053 Msa0383690:CDS 55.0%
CCCCTGCGTGTGTGAATTAC+TGG + chr3_1:86681488-86681507 Msa0383690:intron 55.0%
AGTTGCCCGAACCCATAGTG+TGG + chr3_1:86681583-86681602 Msa0383690:intron 55.0%
TTGTCAGGTGGAGAGAGCTG+TGG + chr3_1:86681756-86681775 Msa0383690:intron 55.0%
CACTGCGACTTCATGCTTGG+TGG + chr3_1:86683417-86683436 Msa0383690:intron 55.0%
TCCACCGTACAGGTCTGTCG+TGG + chr3_1:86680901-86680920 Msa0383690:intron 60.0%
TCCACGACAGACCTGTACGG+TGG - chr3_1:86680905-86680924 None:intergenic 60.0%
AAACGGCCGAGTGCAAGGGA+AGG - chr3_1:86680949-86680968 None:intergenic 60.0%
GTTTAGGTCAGGGGTGGGTG+TGG + chr3_1:86681418-86681437 Msa0383690:intron 60.0%
CTCGCACTTCCGTCACCGTC+AGG + chr3_1:86680814-86680833 Msa0383690:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 86680638 86683932 86680638 ID=Msa0383690;Name=Msa0383690
chr3_1 mRNA 86680638 86683932 86680638 ID=Msa0383690-mRNA-1;Parent=Msa0383690;Name=Msa0383690-mRNA-1;_AED=0.23;_eAED=0.23;_QI=76|1|1|1|0.5|0.33|3|332|90
chr3_1 exon 86680638 86680835 86680638 ID=Msa0383690-mRNA-1:exon:22952;Parent=Msa0383690-mRNA-1
chr3_1 exon 86680913 86681086 86680913 ID=Msa0383690-mRNA-1:exon:22953;Parent=Msa0383690-mRNA-1
chr3_1 exon 86683624 86683932 86683624 ID=Msa0383690-mRNA-1:exon:22954;Parent=Msa0383690-mRNA-1
chr3_1 five_prime_UTR 86680638 86680713 86680638 ID=Msa0383690-mRNA-1:five_prime_utr;Parent=Msa0383690-mRNA-1
chr3_1 CDS 86680714 86680835 86680714 ID=Msa0383690-mRNA-1:cds;Parent=Msa0383690-mRNA-1
chr3_1 CDS 86680913 86681063 86680913 ID=Msa0383690-mRNA-1:cds;Parent=Msa0383690-mRNA-1
chr3_1 three_prime_UTR 86681064 86681086 86681064 ID=Msa0383690-mRNA-1:three_prime_utr;Parent=Msa0383690-mRNA-1
chr3_1 three_prime_UTR 86683624 86683932 86683624 ID=Msa0383690-mRNA-1:three_prime_utr;Parent=Msa0383690-mRNA-1
Gene Sequence

>Msa0383690

ATGGCTTCTCGTTACAGATCATTTTCGCAACCAGCATTCTCTCTAATCAAATCCACCATCACCAAACCCAAATCCTCCTTCTCACCCTTTCTCCTCAAAACTCGCACTTCCGTCACCGTCAGGTCTGTCGTGGAGCTTGGTTGCGTGCAGTCACTCCTTCCCTTGCACTCGGCCGTTTCATCAGCGCGTCTTACTTCTTGCCTCGGAATCGATTCAACAAGTTCGAGATCTTTGTCTCAGGGTATGCTTTGCAGTGCCAACCCTGGTGTTTGA
Protein sequence

>Msa0383690

MASRYRSFSQPAFSLIKSTITKPKSSFSPFLLKTRTSVTVRSVVELGCVQSLLPLHSAVSSARLTSCLGIDSTSSRSLSQGMLCSANPGV*