AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0451150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0451150 MtrunA17_Chr3g0096101 81.944 144 9 2 30 156 107 250 1.91e-66 202
Msa0451150 MtrunA17_Chr3g0096101 57.377 61 21 1 1 61 1 56 3.46e-13 65.1
Msa0451150 MtrunA17_Chr4g0052201 39.506 162 65 5 22 150 92 253 2.95e-29 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0451150 AT2G30890.1 45.736 129 53 2 30 141 101 229 6.19e-33 117
Msa0451150 AT4G18260.1 45.312 128 51 2 30 138 109 236 6.50e-32 115

Find 24 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTAGGGGTTGCACTTTA+TGG 0.214906 3_3:+38593614 Msa0451150:CDS
CATCAAAAGGCATAAGAATT+TGG 0.238501 3_3:+38594290 Msa0451150:CDS
ACAATAATCATCAAAGATTA+GGG 0.240819 3_3:+38593600 Msa0451150:CDS
GAACCATACACTTCTTCTTT+TGG 0.257377 3_3:-38594182 None:intergenic
TTGCACTTTATGGTGTTATC+TGG 0.283526 3_3:+38593624 Msa0451150:CDS
AAGAAATGAACTTTGTTTGC+TGG 0.323312 3_3:-38591996 None:intergenic
AACAATAATCATCAAAGATT+AGG 0.323426 3_3:+38593599 Msa0451150:CDS
GGTGTTCTAAATGTATATAT+TGG 0.364743 3_3:+38594244 Msa0451150:CDS
TGGAACTGCAGTAACATTCT+TGG 0.365502 3_3:+38594222 Msa0451150:intron
TTGAAGGCTGGCACTTGTTA+AGG 0.371264 3_3:+38594445 Msa0451150:CDS
GGAACTGCAGTAACATTCTT+GGG 0.440614 3_3:+38594223 Msa0451150:intron
TGAAAAGGAGATGGTATTGA+AGG 0.474466 3_3:+38594429 Msa0451150:CDS
AGAAAGTCTTCCAAATGAAA+AGG 0.491408 3_3:+38594414 Msa0451150:CDS
AGATCATATGAAAGACATGA+TGG 0.510970 3_3:+38591969 None:intergenic
AAACAAGACATGCTTGAAAG+AGG 0.517737 3_3:-38592020 None:intergenic
GCTGTACTTCTTGCAACAGC+AGG 0.522233 3_3:+38593539 Msa0451150:CDS
GATCCAAAAGAAGAAGTGTA+TGG 0.524463 3_3:+38594179 Msa0451150:CDS
ATCTGGCTGCAAGTCCTAGT+TGG 0.526574 3_3:+38593641 Msa0451150:CDS
TTTGATGATTATTGTTGAAG+AGG 0.545214 3_3:-38593592 None:intergenic
AAGGAGATGGTATTGAAGGC+TGG 0.554773 3_3:+38594433 Msa0451150:CDS
TCTTCCAAATGAAAAGGAGA+TGG 0.597072 3_3:+38594420 Msa0451150:CDS
GATCATATGAAAGACATGAT+GGG 0.603054 3_3:+38591970 None:intergenic
GTGGTCGAATGATACCAACT+AGG 0.678006 3_3:-38593655 None:intergenic
CAATAATCATCAAAGATTAG+GGG 0.720596 3_3:+38593601 Msa0451150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAATATTTAAATATAAT+TGG - chr3_3:38592651-38592670 None:intergenic 0.0%
!!! TATTAATTTAACACTTATTT+TGG - chr3_3:38592683-38592702 None:intergenic 10.0%
!!! TTAAATAATAATCTTTTTGA+TGG + chr3_3:38593243-38593262 Msa0451150:intron 10.0%
!! TTCTTAATGAAAAAAAATTG+AGG - chr3_3:38592315-38592334 None:intergenic 15.0%
!! GAATAAATTAACTAAAAATG+TGG - chr3_3:38593126-38593145 None:intergenic 15.0%
!! AATTATAATCTTTGTCAAAA+AGG - chr3_3:38593194-38593213 None:intergenic 15.0%
!!! AGTTTGTCTTTTTAATATCA+AGG + chr3_3:38592213-38592232 Msa0451150:intron 20.0%
!!! GTTTGTCTTTTTAATATCAA+GGG + chr3_3:38592214-38592233 Msa0451150:intron 20.0%
!! TAATGAAAAAAAATTGAGGA+AGG - chr3_3:38592311-38592330 None:intergenic 20.0%
!!! CAATTTGAAATTACATTACA+TGG + chr3_3:38592396-38592415 Msa0451150:intron 20.0%
!! AATTAGATTATCAAATAGAG+AGG + chr3_3:38592470-38592489 Msa0451150:intron 20.0%
!!! GAATTTATTTTGTATTCTGT+TGG + chr3_3:38592600-38592619 Msa0451150:intron 20.0%
!!! ACTAAAGTATAAAGTATCTT+AGG + chr3_3:38592899-38592918 Msa0451150:intron 20.0%
!! TACATAAATAGGTCAATTAA+AGG - chr3_3:38592950-38592969 None:intergenic 20.0%
!! AAATATTGATAGATCCAAAT+GGG - chr3_3:38593014-38593033 None:intergenic 20.0%
!! AAAATATTGATAGATCCAAA+TGG - chr3_3:38593015-38593034 None:intergenic 20.0%
!! TTACTAATAAGATCATGTAA+TGG + chr3_3:38593159-38593178 Msa0451150:intron 20.0%
!! AGATTATAATTGATACTTTG+AGG + chr3_3:38593203-38593222 Msa0451150:intron 20.0%
!! AACAATAATCATCAAAGATT+AGG + chr3_3:38593599-38593618 Msa0451150:CDS 20.0%
!! ACAATAATCATCAAAGATTA+GGG + chr3_3:38593600-38593619 Msa0451150:CDS 20.0%
!!! AAAACATAATTTTTGTCGTT+AGG + chr3_3:38594045-38594064 Msa0451150:intron 20.0%
! TCAAATTTGATACCTTAATG+TGG - chr3_3:38592122-38592141 None:intergenic 25.0%
! ATCTTCCATATTGAAGATTA+AGG - chr3_3:38592162-38592181 None:intergenic 25.0%
! GTAATTTCAAATTGAAGTCT+TGG - chr3_3:38592390-38592409 None:intergenic 25.0%
!! CTATGTTCATACAATTTTTC+AGG + chr3_3:38592521-38592540 Msa0451150:intron 25.0%
!! CACTGCTTTATTACATAAAT+AGG - chr3_3:38592961-38592980 None:intergenic 25.0%
! CTATTTATGTAATAAAGCAG+TGG + chr3_3:38592959-38592978 Msa0451150:intron 25.0%
! AATATTGATAGATCCAAATG+GGG - chr3_3:38593013-38593032 None:intergenic 25.0%
!!! CATTGGTTAACTTTTAATGA+TGG + chr3_3:38593063-38593082 Msa0451150:intron 25.0%
! TATTAGTAACTAACAAGCTT+TGG - chr3_3:38593148-38593167 None:intergenic 25.0%
!!! TCTTTTTGATGGTAATATGA+AGG + chr3_3:38593254-38593273 Msa0451150:intron 25.0%
!!! TTTTGAGTTTCTGTTAAAAC+AGG + chr3_3:38593511-38593530 Msa0451150:intron 25.0%
!! TTTGATGATTATTGTTGAAG+AGG - chr3_3:38593595-38593614 None:intergenic 25.0%
! CAATAATCATCAAAGATTAG+GGG + chr3_3:38593601-38593620 Msa0451150:CDS 25.0%
!! ATTTAAATGTTTTACCTTCG+TGG - chr3_3:38593677-38593696 None:intergenic 25.0%
!!! TTTTTGTTGTCTTAAACCTT+CGG - chr3_3:38594027-38594046 None:intergenic 25.0%
! GATTTCAATGGTATAAGATT+GGG - chr3_3:38594091-38594110 None:intergenic 25.0%
! TGATTTCAATGGTATAAGAT+TGG - chr3_3:38594092-38594111 None:intergenic 25.0%
!! AGTTTCGAATATCTTTTCAA+TGG - chr3_3:38594134-38594153 None:intergenic 25.0%
!! ATCTTTATTTACTTGTTCAC+AGG + chr3_3:38594155-38594174 Msa0451150:intron 25.0%
!! TCTTTATTTACTTGTTCACA+GGG + chr3_3:38594156-38594175 Msa0451150:intron 25.0%
!! GGTGTTCTAAATGTATATAT+TGG + chr3_3:38594244-38594263 Msa0451150:CDS 25.0%
! TACCATGAAAAAACATCAAA+AGG + chr3_3:38594277-38594296 Msa0451150:CDS 25.0%
!!! TCTACCTTTTTCAAGAAAAA+TGG + chr3_3:38594353-38594372 Msa0451150:CDS 25.0%
!!! CTACCTTTTTCAAGAAAAAT+GGG + chr3_3:38594354-38594373 Msa0451150:CDS 25.0%
! AAGAAATGAACTTTGTTTGC+TGG - chr3_3:38591999-38592018 None:intergenic 30.0%
! CAAGAGCAAAAGGAAATTTT+AGG + chr3_3:38592072-38592091 Msa0451150:intron 30.0%
AAACAATGACAACCACATTA+AGG + chr3_3:38592107-38592126 Msa0451150:intron 30.0%
TCTTGCCTTAATCTTCAATA+TGG + chr3_3:38592154-38592173 Msa0451150:intron 30.0%
AAAGACAAACTCTTAGCTAT+AGG - chr3_3:38592204-38592223 None:intergenic 30.0%
CTAATTGCAAGTATACCAAT+AGG - chr3_3:38592456-38592475 None:intergenic 30.0%
AATAAAATTCTAAGCCACCT+TGG - chr3_3:38592501-38592520 None:intergenic 30.0%
TATCTTAGGTAGATATTCTG+TGG + chr3_3:38592913-38592932 Msa0451150:intron 30.0%
ATTAAGATCAGTTGTCACAT+TGG + chr3_3:38593046-38593065 Msa0451150:intron 30.0%
! AACTTTTAATGATGGCATGA+AGG + chr3_3:38593071-38593090 Msa0451150:intron 30.0%
! ACTTTTAATGATGGCATGAA+GGG + chr3_3:38593072-38593091 Msa0451150:intron 30.0%
CTAAATGTAACCACATATGT+CGG + chr3_3:38593360-38593379 Msa0451150:intron 30.0%
GTTTCATAGACATAAATGGT+GGG - chr3_3:38593787-38593806 None:intergenic 30.0%
CTTGGTTTCATAGACATAAA+TGG - chr3_3:38593791-38593810 None:intergenic 30.0%
!! TTTCTAAGACTAACCTACAT+AGG + chr3_3:38593916-38593935 Msa0451150:intron 30.0%
! TATCTTTTCAATGGTCTACA+TGG - chr3_3:38594125-38594144 None:intergenic 30.0%
! CTTTATTTACTTGTTCACAG+GGG + chr3_3:38594157-38594176 Msa0451150:intron 30.0%
!!! TGCCTTTTGATGTTTTTTCA+TGG - chr3_3:38594282-38594301 None:intergenic 30.0%
! CATCAAAAGGCATAAGAATT+TGG + chr3_3:38594290-38594309 Msa0451150:CDS 30.0%
!! AGACCCATTTTTCTTGAAAA+AGG - chr3_3:38594360-38594379 None:intergenic 30.0%
CTACATACAAAAACAAAGAG+TGG + chr3_3:38594378-38594397 Msa0451150:CDS 30.0%
AGAAAGTCTTCCAAATGAAA+AGG + chr3_3:38594414-38594433 Msa0451150:CDS 30.0%
! AATACCATCTCCTTTTCATT+TGG - chr3_3:38594427-38594446 None:intergenic 30.0%
AAACAAGACATGCTTGAAAG+AGG - chr3_3:38592023-38592042 None:intergenic 35.0%
TCTTGTGATGAACTAACAAG+TGG - chr3_3:38592057-38592076 None:intergenic 35.0%
TAGTTCATCACAAGAGCAAA+AGG + chr3_3:38592062-38592081 Msa0451150:intron 35.0%
ATTACATGGCTTTCTCCTTT+GGG + chr3_3:38592410-38592429 Msa0451150:intron 35.0%
GATTGTCTTGTCACATAGAT+AGG - chr3_3:38592748-38592767 None:intergenic 35.0%
! TAAAGCAGTGGTGAAAGTTT+TGG + chr3_3:38592971-38592990 Msa0451150:intron 35.0%
!! AATTTTCTTGCTGTCTTCAC+AGG - chr3_3:38593308-38593327 None:intergenic 35.0%
TGAGTTTCTGTTAAAACAGG+CGG + chr3_3:38593514-38593533 Msa0451150:intron 35.0%
!! TTGCACTTTATGGTGTTATC+TGG + chr3_3:38593624-38593643 Msa0451150:CDS 35.0%
GTGAGCATTTCTCAACAAAA+GGG - chr3_3:38593701-38593720 None:intergenic 35.0%
AGTGAGCATTTCTCAACAAA+AGG - chr3_3:38593702-38593721 None:intergenic 35.0%
! TGCTATCAGGTTTTCGTTAT+CGG + chr3_3:38593757-38593776 Msa0451150:intron 35.0%
GGTTTCATAGACATAAATGG+TGG - chr3_3:38593788-38593807 None:intergenic 35.0%
!! ACCTTACAAGCCGATTTTAT+GGG + chr3_3:38593871-38593890 Msa0451150:intron 35.0%
CTAACACAACCCCATAAAAT+CGG - chr3_3:38593884-38593903 None:intergenic 35.0%
!! TAGGTTAGTCTTAGAAATCG+TGG - chr3_3:38593913-38593932 None:intergenic 35.0%
ACTAACCTACATAGGTTTGT+CGG + chr3_3:38593924-38593943 Msa0451150:intron 35.0%
TTATGATATAGTCCGGAGTT+CGG - chr3_3:38593988-38594007 None:intergenic 35.0%
GAAGGGATTATGATATAGTC+CGG - chr3_3:38593995-38594014 None:intergenic 35.0%
TGGTATAAGATTGGGATTTC+AGG - chr3_3:38594083-38594102 None:intergenic 35.0%
! GATCCAAAAGAAGAAGTGTA+TGG + chr3_3:38594179-38594198 Msa0451150:CDS 35.0%
! GAACCATACACTTCTTCTTT+TGG - chr3_3:38594185-38594204 None:intergenic 35.0%
TTCTTTGCACATTGGATACT+TGG + chr3_3:38594202-38594221 Msa0451150:intron 35.0%
TCTTCCAAATGAAAAGGAGA+TGG + chr3_3:38594420-38594439 Msa0451150:CDS 35.0%
! TGAAAAGGAGATGGTATTGA+AGG + chr3_3:38594429-38594448 Msa0451150:CDS 35.0%
CATTACATGGCTTTCTCCTT+TGG + chr3_3:38592409-38592428 Msa0451150:intron 40.0%
TTTCTCCTTTGGGCTTCAAT+GGG + chr3_3:38592420-38592439 Msa0451150:intron 40.0%
ATGGGATTCTTGATGCCTAT+TGG + chr3_3:38592438-38592457 Msa0451150:intron 40.0%
CAAATAGAGAGGAGAATCCA+AGG + chr3_3:38592481-38592500 Msa0451150:intron 40.0%
!! GTGGTGAAAGTTTTGGTAGA+TGG + chr3_3:38592978-38592997 Msa0451150:intron 40.0%
!! GTTTTGGTAGATGGTTACTC+TGG + chr3_3:38592987-38593006 Msa0451150:intron 40.0%
AGATTAGGGGTTGCACTTTA+TGG + chr3_3:38593614-38593633 Msa0451150:CDS 40.0%
GATAACGAAAACCTGATAGC+AGG - chr3_3:38593758-38593777 None:intergenic 40.0%
! TCATAGACATAAATGGTGGG+TGG - chr3_3:38593784-38593803 None:intergenic 40.0%
ACGACTAGCACTATTGAACT+TGG - chr3_3:38593809-38593828 None:intergenic 40.0%
GTGTTAAAAAGTCCCACAAG+TGG + chr3_3:38593837-38593856 Msa0451150:intron 40.0%
!! TAAAATCGGCTTGTAAGGTG+AGG - chr3_3:38593870-38593889 None:intergenic 40.0%
!! CACCTTACAAGCCGATTTTA+TGG + chr3_3:38593870-38593889 Msa0451150:intron 40.0%
!! CCTTACAAGCCGATTTTATG+GGG + chr3_3:38593872-38593891 Msa0451150:intron 40.0%
TTCAACCGACAAACCTATGT+AGG - chr3_3:38593932-38593951 None:intergenic 40.0%
TTCGGACGATAGGTAAGTAT+AGG - chr3_3:38593970-38593989 None:intergenic 40.0%
GCAAGAAAGGGTGATTTCAA+TGG - chr3_3:38594103-38594122 None:intergenic 40.0%
ATGGTCTACATGGCAAGAAA+GGG - chr3_3:38594115-38594134 None:intergenic 40.0%
AATGGTCTACATGGCAAGAA+AGG - chr3_3:38594116-38594135 None:intergenic 40.0%
GTGTATGGTTCTTTGCACAT+TGG + chr3_3:38594194-38594213 Msa0451150:intron 40.0%
TGGAACTGCAGTAACATTCT+TGG + chr3_3:38594222-38594241 Msa0451150:intron 40.0%
GGAACTGCAGTAACATTCTT+GGG + chr3_3:38594223-38594242 Msa0451150:intron 40.0%
CTTTCTCCTTTGGGCTTCAA+TGG + chr3_3:38592419-38592438 Msa0451150:intron 45.0%
AGAATCCCATTGAAGCCCAA+AGG - chr3_3:38592428-38592447 None:intergenic 45.0%
ATAGAGAGGAGAATCCAAGG+TGG + chr3_3:38592484-38592503 Msa0451150:intron 45.0%
ATGACACACACCGACATATG+TGG - chr3_3:38593373-38593392 None:intergenic 45.0%
GTGGTCGAATGATACCAACT+AGG - chr3_3:38593658-38593677 None:intergenic 45.0%
TTGGTATCATTCGACCACGA+AGG + chr3_3:38593660-38593679 Msa0451150:intron 45.0%
TCATTCGATCACCTGCTATC+AGG + chr3_3:38593744-38593763 Msa0451150:intron 45.0%
TGAGGATTGTCTCCACTTGT+GGG - chr3_3:38593852-38593871 None:intergenic 45.0%
!! CCCCATAAAATCGGCTTGTA+AGG - chr3_3:38593875-38593894 None:intergenic 45.0%
CTTAAACCTTCGGTTCTCAG+AGG - chr3_3:38594017-38594036 None:intergenic 45.0%
! AAGGAGATGGTATTGAAGGC+TGG + chr3_3:38594433-38594452 Msa0451150:CDS 45.0%
ATGGTTACTCTGGCCCCATT+TGG + chr3_3:38592997-38593016 Msa0451150:intron 50.0%
GCTGTACTTCTTGCAACAGC+AGG + chr3_3:38593539-38593558 Msa0451150:CDS 50.0%
ATCTGGCTGCAAGTCCTAGT+TGG + chr3_3:38593641-38593660 Msa0451150:CDS 50.0%
GTGAGGATTGTCTCCACTTG+TGG - chr3_3:38593853-38593872 None:intergenic 50.0%
CTTACCTATCGTCCGAACTC+CGG + chr3_3:38593973-38593992 Msa0451150:intron 50.0%
ACCTTCGGTTCTCAGAGGAA+GGG - chr3_3:38594012-38594031 None:intergenic 50.0%
AACCTTCGGTTCTCAGAGGA+AGG - chr3_3:38594013-38594032 None:intergenic 50.0%
TAGTCCGGAGTTCGGACGAT+AGG - chr3_3:38593980-38593999 None:intergenic 55.0%
! TCCCTTCCTCTGAGAACCGA+AGG + chr3_3:38594008-38594027 Msa0451150:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 38591976 38594465 38591976 ID=Msa0451150;Name=Msa0451150
chr3_3 mRNA 38591976 38594465 38591976 ID=Msa0451150-mRNA-1;Parent=Msa0451150;Name=Msa0451150-mRNA-1;_AED=0.38;_eAED=0.41;_QI=0|0|0|1|0|0|4|0|156
chr3_3 exon 38591976 38592059 38591976 ID=Msa0451150-mRNA-1:exon:6932;Parent=Msa0451150-mRNA-1
chr3_3 exon 38593533 38593662 38593533 ID=Msa0451150-mRNA-1:exon:6933;Parent=Msa0451150-mRNA-1
chr3_3 exon 38594177 38594200 38594177 ID=Msa0451150-mRNA-1:exon:6934;Parent=Msa0451150-mRNA-1
chr3_3 exon 38594233 38594465 38594233 ID=Msa0451150-mRNA-1:exon:6935;Parent=Msa0451150-mRNA-1
chr3_3 CDS 38591976 38592059 38591976 ID=Msa0451150-mRNA-1:cds;Parent=Msa0451150-mRNA-1
chr3_3 CDS 38593533 38593662 38593533 ID=Msa0451150-mRNA-1:cds;Parent=Msa0451150-mRNA-1
chr3_3 CDS 38594177 38594200 38594177 ID=Msa0451150-mRNA-1:cds;Parent=Msa0451150-mRNA-1
chr3_3 CDS 38594233 38594465 38594233 ID=Msa0451150-mRNA-1:cds;Parent=Msa0451150-mRNA-1
Gene Sequence

>Msa0451150

ATGAAAGACATGATGGGAATCCAGCAAACAAAGTTCATTTCTTTCCTCTTTCAAGCATGTCTTGTTTTGTTCTTATTTCCACTTGCGGTTGCTGTACTTCTTGCAACAGCAGGAGCAATCATGTCAATAAAAAATTTCAACAACCTCTTCAACAATAATCATCAAAGATTAGGGGTTGCACTTTATGGTGTTATCTGGCTGCAAGTCCTAGTTGGGGATCCAAAAGAAGAAGTGTATGTAACATTCTTGGGTGTTCTAAATGTATATATTGGTTTAGCAGCTTACCATGAAAAAACATCAAAAGGCATAAGAATTTGGAATATACTTTTCACTATTCAGATATCATTGATTGTATTTTTCTACCTTTTTCAAGAAAAATGGGTCTACATACAAAAACAAAGAGTGGTTTTGAGCAATGAAGAAAGTCTTCCAAATGAAAAGGAGATGGTATTGAAGGCTGGCACTTGTTAA

Protein sequence

>Msa0451150

MKDMMGIQQTKFISFLFQACLVLFLFPLAVAVLLATAGAIMSIKNFNNLFNNNHQRLGVALYGVIWLQVLVGDPKEEVYVTFLGVLNVYIGLAAYHEKTSKGIRIWNILFTIQISLIVFFYLFQEKWVYIQKQRVVLSNEESLPNEKEMVLKAGTC*