AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0547990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0547990 MtrunA17_Chr4g0030911 83.794 253 2 3 1 253 1 214 4.72e-147 409
Msa0547990 MtrunA17_Chr1g0182321 77.165 254 18 4 1 253 1 215 6.94e-136 381
Msa0547990 MtrunA17_Chr1g0182301 76.772 254 19 4 1 253 1 215 4.05e-135 379
Msa0547990 MtrunA17_Chr1g0212521 78.279 244 13 4 11 253 11 215 1.88e-128 362
Msa0547990 MtrunA17_Chr7g0263071 79.098 244 11 4 11 253 11 215 3.87e-125 354
Msa0547990 MtrunA17_Chr2g0327911 63.386 254 51 5 1 253 1 213 1.95e-104 301
Msa0547990 MtrunA17_Chr7g0250531 52.869 244 66 7 11 252 4 200 1.25e-75 228
Msa0547990 MtrunA17_Chr4g0055651 50.820 244 71 7 11 252 4 200 2.82e-74 224
Msa0547990 MtrunA17_Chr8g0335471 51.429 245 69 7 11 252 4 201 1.03e-72 221
Msa0547990 MtrunA17_Chr2g0276931 47.131 244 83 6 11 253 5 203 9.29e-68 208
Msa0547990 MtrunA17_Chr6g0458081 40.323 248 101 6 12 252 3 210 9.28e-50 162
Msa0547990 MtrunA17_Chr2g0290891 39.516 248 103 5 12 252 3 210 2.20e-49 161
Msa0547990 MtrunA17_Chr4g0032201 48.171 164 72 3 7 170 4 154 1.77e-48 159
Msa0547990 MtrunA17_Chr3g0112681 38.889 252 99 6 11 252 8 214 2.81e-48 159
Msa0547990 MtrunA17_Chr2g0315551 50.000 164 69 3 7 170 5 155 1.11e-46 154
Msa0547990 MtrunA17_Chr2g0276941 48.125 160 70 3 11 170 11 157 1.15e-46 155
Msa0547990 MtrunA17_Chr1g0189361 48.734 158 68 3 13 170 12 156 1.35e-46 154
Msa0547990 MtrunA17_Chr2g0283131 39.526 253 104 7 7 252 5 215 1.63e-46 154
Msa0547990 MtrunA17_Chr3g0123821 35.573 253 115 6 8 252 9 221 5.65e-46 153
Msa0547990 MtrunA17_Chr2g0319951 46.951 164 74 2 7 170 5 155 1.88e-45 151
Msa0547990 MtrunA17_Chr1g0180291 40.083 242 102 6 12 252 11 210 2.47e-45 151
Msa0547990 MtrunA17_Chr4g0046261 38.340 253 107 7 7 252 5 215 2.57e-45 151
Msa0547990 MtrunA17_Chr4g0040781 49.390 164 70 3 7 170 5 155 2.90e-45 151
Msa0547990 MtrunA17_Chr8g0381841 46.203 158 72 3 13 170 15 159 6.88e-45 150
Msa0547990 MtrunA17_Chr8g0349711 36.948 249 105 6 14 252 5 211 1.42e-44 149
Msa0547990 MtrunA17_Chr7g0265001 38.843 242 106 5 12 252 13 213 3.24e-44 148
Msa0547990 MtrunA17_Chr7g0234201 36.667 240 114 6 14 252 12 214 1.53e-42 144
Msa0547990 MtrunA17_Chr3g0130271 46.875 160 72 3 11 169 8 155 1.60e-42 144
Msa0547990 MtrunA17_Chr8g0349681 36.179 246 115 4 9 252 378 583 2.13e-42 152
Msa0547990 MtrunA17_Chr8g0349681 36.752 234 102 5 12 241 172 363 3.43e-38 140
Msa0547990 MtrunA17_Chr8g0349681 44.586 157 74 2 14 170 12 155 9.03e-37 136
Msa0547990 MtrunA17_Chr8g0384311 45.455 165 76 4 6 169 4 155 2.24e-42 143
Msa0547990 MtrunA17_Chr1g0197331 35.827 254 118 6 1 252 1 211 2.40e-42 143
Msa0547990 MtrunA17_Chr1g0159481 39.184 245 102 10 11 252 13 213 2.95e-42 143
Msa0547990 MtrunA17_Chr2g0325091 37.200 250 105 7 11 252 7 212 6.88e-41 140
Msa0547990 MtrunA17_Chr1g0174281 34.902 255 108 6 13 253 13 223 9.46e-41 139
Msa0547990 MtrunA17_Chr8g0351861 45.570 158 73 2 13 170 14 158 2.36e-40 139
Msa0547990 MtrunA17_Chr8g0346421 43.671 158 76 2 13 170 24 168 2.40e-40 139
Msa0547990 MtrunA17_Chr7g0214241 34.286 245 116 7 14 252 14 219 8.86e-40 137
Msa0547990 MtrunA17_Chr7g0275991 40.854 164 84 2 7 170 12 162 3.99e-39 135
Msa0547990 MtrunA17_Chr7g0231131 42.405 158 78 2 13 170 13 157 4.21e-38 132
Msa0547990 MtrunA17_Chr6g0482721 38.235 204 86 4 13 216 10 173 6.74e-37 129
Msa0547990 MtrunA17_Chr8g0392931 37.600 250 102 7 13 252 12 217 1.86e-35 125
Msa0547990 MtrunA17_Chr8g0390561 33.469 245 113 6 8 252 2 196 9.86e-35 123
Msa0547990 MtrunA17_Chr1g0199331 36.275 204 89 4 16 218 32 195 1.62e-34 122
Msa0547990 MtrunA17_Chr3g0109721 32.203 236 112 5 17 252 12 199 1.89e-33 120
Msa0547990 MtrunA17_Chr8g0382221 40.000 155 78 3 17 170 11 151 2.53e-32 117
Msa0547990 MtrunA17_Chr5g0433561 30.932 236 115 5 17 252 12 199 6.12e-32 116
Msa0547990 MtrunA17_Chr5g0400131 30.645 248 127 5 5 252 3 205 7.62e-31 113
Msa0547990 MtrunA17_Chr3g0123971 39.355 155 79 3 17 170 11 151 1.83e-30 112
Msa0547990 MtrunA17_Chr4g0055081 36.747 166 86 4 5 169 3 150 3.40e-29 109
Msa0547990 MtrunA17_Chr4g0034871 32.794 247 111 9 5 241 2 203 7.14e-29 108
Msa0547990 MtrunA17_Chr8g0383261 30.544 239 121 5 15 251 12 207 2.68e-26 102
Msa0547990 MtrunA17_Chr6g0466301 32.178 202 100 4 15 214 8 174 2.81e-26 101
Msa0547990 MtrunA17_Chr6g0465491 32.178 202 100 4 15 214 8 174 2.81e-26 101
Msa0547990 MtrunA17_Chr2g0331691 31.429 245 114 8 15 252 10 207 1.02e-25 100
Msa0547990 MtrunA17_Chr4g0004661 31.837 245 113 8 15 252 64 261 1.08e-25 101
Msa0547990 MtrunA17_Chr1g0201121 29.870 231 123 4 15 241 8 203 2.54e-25 99.4
Msa0547990 MtrunA17_Chr4g0046211 31.111 135 80 3 15 149 6 127 1.54e-18 79.7
Msa0547990 MtrunA17_Chr4g0037021 28.922 204 115 5 15 218 6 179 2.39e-18 80.5
Msa0547990 MtrunA17_Chr2g0325101 28.718 195 109 5 15 209 6 170 4.16e-18 79.7
Msa0547990 MtrunA17_Chr8g0371501 30.000 150 85 4 15 157 6 142 9.78e-18 79.0
Msa0547990 MtrunA17_Chr3g0117931 28.090 178 98 5 15 175 8 172 1.16e-17 79.0
Msa0547990 MtrunA17_Chr5g0405791 30.000 150 85 4 15 157 6 142 1.79e-17 78.2
Msa0547990 MtrunA17_Chr6g0466161 31.000 200 101 5 32 229 7 171 1.90e-17 78.2
Msa0547990 MtrunA17_Chr1g0178291 37.107 159 78 4 18 174 431 569 2.42e-17 81.3
Msa0547990 MtrunA17_Chr6g0465471 31.892 185 89 5 32 214 7 156 6.36e-17 77.0
Msa0547990 MtrunA17_Chr6g0486041 29.744 195 107 7 15 209 6 170 1.34e-16 75.9
Msa0547990 MtrunA17_Chr3g0138121 25.472 212 114 7 5 216 4 171 6.62e-15 71.6
Msa0547990 MtrunA17_Chr3g0138111 25.472 212 114 7 5 216 4 171 8.34e-15 71.2
Msa0547990 MtrunA17_Chr3g0138101 25.472 212 114 7 5 216 4 171 8.42e-15 71.2
Msa0547990 MtrunA17_Chr6g0466431 35.165 91 50 1 15 105 8 89 8.09e-14 65.9
Msa0547990 MtrunA17_Chr6g0465611 35.165 91 50 1 15 105 8 89 8.09e-14 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0547990 AT5G59840.1 78.279 244 13 4 11 253 11 215 1.40e-129 365
Msa0547990 AT3G46060.2 77.869 244 14 4 11 253 11 215 2.14e-129 365
Msa0547990 AT3G46060.3 77.869 244 14 4 11 253 11 215 2.14e-129 365
Msa0547990 AT3G46060.1 77.869 244 14 4 11 253 11 215 2.14e-129 365
Msa0547990 AT3G53610.2 77.049 244 16 4 11 253 11 215 3.57e-127 359
Msa0547990 AT3G53610.3 77.049 244 16 4 11 253 11 215 3.57e-127 359
Msa0547990 AT3G53610.1 77.049 244 16 4 11 253 11 215 3.57e-127 359
Msa0547990 AT5G03520.1 72.222 252 31 3 1 252 1 213 5.65e-117 333
Msa0547990 AT3G09900.1 70.866 254 33 4 1 252 1 215 1.74e-113 325
Msa0547990 AT5G03520.2 67.980 203 26 3 50 252 40 203 4.77e-90 265
Msa0547990 AT1G02130.1 52.653 245 66 7 11 252 4 201 2.10e-77 233
Msa0547990 AT5G47200.1 50.410 244 72 7 11 252 4 200 1.43e-70 215
Msa0547990 AT4G17530.1 50.000 244 73 7 11 252 4 200 2.88e-70 214
Msa0547990 AT3G11730.1 48.387 248 76 7 11 253 4 204 1.78e-68 210
Msa0547990 AT4G35860.1 41.365 249 99 6 12 253 3 211 9.02e-54 173
Msa0547990 AT4G17160.1 39.754 244 104 5 12 253 3 205 1.26e-51 167
Msa0547990 AT4G17170.1 39.759 249 103 5 12 253 3 211 2.84e-51 166
Msa0547990 AT5G59150.1 48.171 164 72 3 7 170 4 154 2.05e-48 159
Msa0547990 AT1G16920.1 49.390 164 70 3 7 170 5 155 1.02e-47 157
Msa0547990 AT3G46830.1 48.171 164 72 3 7 170 4 154 1.34e-47 157
Msa0547990 AT3G46830.2 48.171 164 72 3 7 170 4 154 1.34e-47 157
Msa0547990 AT1G09630.1 39.044 251 107 6 7 251 4 214 1.42e-47 157
Msa0547990 AT1G07410.1 46.951 164 74 3 7 170 4 154 5.63e-47 155
Msa0547990 AT4G18430.1 37.849 251 111 6 7 252 5 215 3.46e-46 154
Msa0547990 AT4G39990.1 37.247 247 108 6 13 252 15 221 1.02e-45 152
Msa0547990 AT3G15060.1 48.780 164 71 3 7 170 5 155 9.58e-45 150
Msa0547990 AT5G60860.1 38.340 253 107 7 7 252 5 215 1.64e-44 149
Msa0547990 AT1G06400.1 46.341 164 75 2 7 170 5 155 2.23e-44 149
Msa0547990 AT3G07410.1 37.959 245 108 7 13 252 10 215 4.33e-44 148
Msa0547990 AT1G28550.1 48.171 164 72 3 7 170 5 155 6.23e-44 148
Msa0547990 AT5G47520.1 48.101 158 69 3 13 170 12 156 6.56e-44 148
Msa0547990 AT5G45750.1 46.951 164 74 3 7 170 5 155 7.06e-44 148
Msa0547990 AT1G05810.1 46.203 158 72 3 13 170 10 154 8.79e-44 147
Msa0547990 AT4G18800.1 47.561 164 73 3 7 170 5 155 8.93e-44 147
Msa0547990 AT1G05810.2 46.203 158 72 3 13 170 56 200 1.81e-43 148
Msa0547990 AT2G33870.1 38.431 255 107 8 7 253 5 217 7.02e-43 145
Msa0547990 AT2G31680.1 45.570 158 73 3 13 170 10 154 9.15e-43 145
Msa0547990 AT5G47960.1 46.203 158 72 2 13 170 13 157 1.13e-42 145
Msa0547990 AT2G43130.1 45.570 158 73 3 13 170 10 154 1.41e-42 144
Msa0547990 AT1G01200.1 40.385 208 85 4 13 220 26 194 2.78e-42 144
Msa0547990 AT5G65270.1 43.671 158 76 3 13 170 15 159 3.88e-42 144
Msa0547990 AT1G43890.3 41.463 205 79 6 6 209 4 168 1.69e-41 141
Msa0547990 AT1G43890.1 41.463 205 79 6 6 209 4 168 1.69e-41 141
Msa0547990 AT1G43890.2 41.463 205 79 6 6 209 4 168 1.69e-41 141
Msa0547990 AT5G03530.2 37.805 246 110 6 8 252 6 209 2.76e-41 141
Msa0547990 AT5G03530.1 37.805 246 110 6 8 252 6 209 2.76e-41 141
Msa0547990 AT3G12160.1 33.871 248 117 4 13 253 13 220 4.16e-41 140
Msa0547990 AT1G18200.1 40.385 208 84 4 13 220 11 178 4.76e-39 135
Msa0547990 AT1G73640.1 38.326 227 96 5 13 235 11 197 6.49e-39 135
Msa0547990 AT3G54840.2 43.787 169 78 5 3 170 24 176 3.28e-38 132
Msa0547990 AT3G54840.1 43.787 169 78 5 3 170 24 176 3.91e-38 132
Msa0547990 AT3G54840.3 44.805 154 71 4 18 170 2 142 5.95e-37 128
Msa0547990 AT3G09910.5 38.696 230 100 7 8 236 6 195 5.04e-36 127
Msa0547990 AT3G09910.4 38.696 230 100 7 8 236 6 195 5.04e-36 127
Msa0547990 AT3G09910.3 43.684 190 83 7 8 186 6 182 4.00e-35 125
Msa0547990 AT3G09910.1 43.684 190 83 7 8 186 6 182 4.00e-35 125
Msa0547990 AT3G09910.2 43.684 190 83 7 8 186 6 182 4.00e-35 125
Msa0547990 AT4G35860.2 38.235 204 79 6 57 253 2 165 4.75e-34 121
Msa0547990 AT4G19640.1 33.333 237 110 7 17 253 12 200 1.29e-32 118
Msa0547990 AT5G45130.1 30.802 237 116 5 17 253 12 200 3.30e-32 117
Msa0547990 AT2G22290.1 31.356 236 121 5 17 252 11 205 8.27e-32 116
Msa0547990 AT4G39890.1 34.848 198 88 5 17 213 11 168 1.06e-30 114
Msa0547990 AT5G45130.2 30.638 235 115 5 19 253 22 208 1.48e-30 113
Msa0547990 AT5G10260.1 30.802 237 123 5 17 253 11 206 1.56e-30 113
Msa0547990 AT3G18820.1 33.603 247 109 9 5 241 2 203 5.22e-30 112
Msa0547990 AT5G59150.2 47.368 114 47 3 57 170 3 103 4.56e-29 108
Msa0547990 AT2G44610.1 36.145 166 89 3 5 170 3 151 4.24e-28 107
Msa0547990 AT5G64990.1 37.662 154 83 2 17 170 9 149 2.71e-27 104
Msa0547990 AT1G49300.2 37.288 177 87 6 5 174 2 161 3.04e-27 104
Msa0547990 AT1G49300.1 37.288 177 87 6 5 174 2 161 3.04e-27 104
Msa0547990 AT1G52280.1 39.264 163 79 5 15 170 8 157 5.56e-27 103
Msa0547990 AT1G22740.1 33.184 223 104 6 15 231 8 191 2.63e-26 102
Msa0547990 AT2G21880.1 31.000 200 105 4 15 214 9 175 6.73e-26 101
Msa0547990 AT4G19640.2 36.090 133 73 1 17 149 12 132 1.27e-25 98.6
Msa0547990 AT4G09720.4 35.294 204 96 5 15 214 8 179 1.30e-25 100
Msa0547990 AT4G09720.1 32.178 202 100 3 15 214 8 174 2.01e-25 99.8
Msa0547990 AT3G16100.1 38.037 163 81 4 15 170 8 157 2.58e-25 99.8
Msa0547990 AT4G19640.3 36.090 133 73 1 17 149 12 132 6.30e-25 98.2
Msa0547990 AT4G09720.3 34.146 205 103 4 15 214 8 185 8.92e-25 98.6
Msa0547990 AT5G64990.2 36.025 161 83 3 17 170 9 156 1.39e-24 97.8
Msa0547990 AT5G39620.1 30.882 204 98 4 16 214 6 171 1.07e-23 95.1
Msa0547990 AT4G35020.1 30.256 195 106 5 15 209 6 170 8.29e-20 84.7
Msa0547990 AT4G35020.2 30.256 195 106 5 15 209 6 170 8.29e-20 84.7
Msa0547990 AT4G35020.3 30.256 195 106 5 15 209 6 170 8.29e-20 84.7
Msa0547990 AT5G62880.1 28.502 207 118 5 15 221 8 184 4.54e-19 83.2
Msa0547990 AT3G48040.2 28.022 182 104 5 15 182 8 176 6.16e-19 82.0
Msa0547990 AT5G45970.1 28.718 195 109 5 15 209 6 170 1.72e-18 81.3
Msa0547990 AT3G48040.1 28.834 163 94 4 15 168 8 157 3.59e-18 80.5
Msa0547990 AT2G21880.4 29.714 175 90 4 40 214 9 150 4.58e-18 79.7
Msa0547990 AT2G21880.3 29.714 175 90 4 40 214 9 150 4.58e-18 79.7
Msa0547990 AT2G17800.2 29.213 178 96 6 15 175 6 170 7.51e-18 79.3
Msa0547990 AT2G17800.1 29.213 178 96 6 15 175 6 170 7.51e-18 79.3
Msa0547990 AT3G51300.1 28.931 159 91 5 15 164 6 151 1.24e-17 79.0
Msa0547990 AT4G35950.1 29.213 178 96 6 15 175 6 170 1.61e-17 78.6
Msa0547990 AT1G75840.1 29.801 151 86 4 15 158 6 143 1.75e-17 78.6
Msa0547990 AT2G44690.1 28.302 159 92 4 15 164 18 163 2.37e-17 78.6
Msa0547990 AT5G62880.2 30.189 159 90 4 15 173 8 145 2.38e-17 78.2
Msa0547990 AT1G20090.1 29.801 151 86 4 15 158 5 142 2.58e-17 78.2
Msa0547990 AT4G09720.6 31.214 173 82 3 44 214 3 140 1.41e-16 75.5
Msa0547990 AT4G09720.5 31.214 173 82 3 44 214 3 140 1.41e-16 75.5
Msa0547990 AT4G09720.2 31.214 173 82 3 44 214 3 140 1.41e-16 75.5
Msa0547990 AT2G21880.2 27.000 200 105 5 15 214 9 167 2.53e-16 75.5
Msa0547990 AT4G28950.1 30.573 157 89 6 15 164 6 149 3.73e-16 75.1
Msa0547990 AT4G35020.4 33.071 127 72 3 15 141 6 119 8.81e-16 72.8
Msa0547990 AT4G35020.5 33.071 127 72 3 15 141 6 119 8.81e-16 72.8
Msa0547990 AT5G55080.1 25.871 201 106 6 16 216 14 171 1.26e-13 68.6

Find 78 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATATTATACCACAATTAT+AGG 0.240269 4_1:-41578736 Msa0547990:five_prime_UTR
AACGTATCAAGCTCCAAATT+TGG 0.259101 4_1:-41577169 Msa0547990:CDS
ACCAGTTTCATCACAACTAT+TGG 0.260192 4_1:-41577314 Msa0547990:CDS
GTCCTGCAGTATCCCAAATT+TGG 0.278971 4_1:+41577156 None:intergenic
ATCAGCCAGTGCTTGGCCTT+TGG 0.327266 4_1:+41576303 None:intergenic
TCTAACTACCTCTGTTTGAT+TGG 0.332170 4_1:-41574845 Msa0547990:three_prime_UTR
CAAGACTCTGCAACGGGGTC+TGG 0.345479 4_1:-41575081 Msa0547990:CDS
ACCTCTGTTTGATTGGGATT+AGG 0.371672 4_1:-41574838 Msa0547990:three_prime_UTR
ACGTATCAAGCTCCAAATTT+GGG 0.372566 4_1:-41577168 Msa0547990:CDS
ATAAGAACCATTGAACTTGA+TGG 0.385351 4_1:-41577194 Msa0547990:CDS
TTGTGCGTGATAAGTAGTTT+TGG 0.385858 4_1:-41574914 Msa0547990:three_prime_UTR
ATCAACCAAGACTCTGCAAC+GGG 0.391757 4_1:-41575087 Msa0547990:CDS
CCTTACCTGTTGTAATTGTC+CGG 0.397162 4_1:+41577123 None:intergenic
CCAGTTTCATCACAACTATT+GGG 0.400381 4_1:-41577313 Msa0547990:intron
TGGTGCCATGGGCATATTGC+TGG 0.408906 4_1:-41576951 Msa0547990:CDS
CAAGCACTGGCTGATGAGTA+TGG 0.410586 4_1:-41576295 Msa0547990:CDS
TGCGGGTGCAAGTCTGTATC+TGG 0.413956 4_1:-41578846 None:intergenic
CTTAATGAGGTAATCGTAAT+CGG 0.415779 4_1:+41578388 None:intergenic
AGTCTGTATCTGGCTTCATA+AGG 0.419229 4_1:-41578836 Msa0547990:five_prime_UTR
TAATCGGCACGAGCCCTTGC+CGG 0.419982 4_1:+41578404 None:intergenic
CTAACTACCTCTGTTTGATT+GGG 0.420571 4_1:-41574844 Msa0547990:three_prime_UTR
CTATGCGTTGATAGATGCTT+CGG 0.422562 4_1:-41574715 Msa0547990:three_prime_UTR
GGTAGGGAACAAAGCAGATA+TGG 0.426102 4_1:-41576714 Msa0547990:CDS
GGATAATGTTAACAAAATAC+TGG 0.430059 4_1:-41576735 Msa0547990:CDS
CTCATTAAGCTTCTCTTGAT+CGG 0.432261 4_1:-41578374 Msa0547990:CDS
GTCTGTATCTGGCTTCATAA+GGG 0.433509 4_1:-41578835 Msa0547990:five_prime_UTR
CCCAATAGTTGTGATGAAAC+TGG 0.436614 4_1:+41577313 None:intergenic
CAACATTGAGCAACATGCTT+CGG 0.444470 4_1:-41576756 Msa0547990:CDS
CAGATATGGACGAAAGCAAA+AGG 0.446902 4_1:-41576700 Msa0547990:CDS
ACGTTTGCCATCAAGTTCAA+TGG 0.447823 4_1:+41577187 None:intergenic
GATGGCTGCTCCACCGGCAA+GGG 0.449585 4_1:-41578417 Msa0547990:CDS
CTCTGCAACGGGGTCTGGAC+AGG 0.453418 4_1:-41575076 Msa0547990:CDS
CTGCAGGACAGGAACGGTTC+CGG 0.459289 4_1:-41577142 Msa0547990:CDS
AGCTTACTATCGTGGTGCCA+TGG 0.468786 4_1:-41576963 Msa0547990:CDS
TGATGGCTGCTCCACCGGCA+AGG 0.468923 4_1:-41578418 Msa0547990:CDS
CAGACCCCGTTGCAGAGTCT+TGG 0.475391 4_1:+41575082 None:intergenic
AACAGTAATGTTCCTTTCTG+TGG 0.484279 4_1:-41576899 Msa0547990:CDS
TGTATAAACTTGCTTATCAA+TGG 0.489406 4_1:-41574796 Msa0547990:three_prime_UTR
AAATCTGCTTGCTGTGGTTG+AGG 0.490381 4_1:-41575045 Msa0547990:exon
GCTTACTATCGTGGTGCCAT+GGG 0.506910 4_1:-41576962 Msa0547990:CDS
AACGTCCAAAGGCCAAGCAC+TGG 0.513872 4_1:-41576308 Msa0547990:CDS
AACGCATAGTTAGTAGCTTG+TGG 0.522675 4_1:+41574658 None:intergenic
CGGTTCCGGACAATTACAAC+AGG 0.522865 4_1:-41577128 Msa0547990:intron
AATGTTAACAAAATACTGGT+AGG 0.531919 4_1:-41576731 Msa0547990:CDS
AGTGCTTGGCCTTTGGACGT+TGG 0.532422 4_1:+41576310 None:intergenic
ACGCATAGTTAGTAGCTTGT+GGG 0.533460 4_1:+41574659 None:intergenic
CATACTCATCAGCCAGTGCT+TGG 0.535062 4_1:+41576296 None:intergenic
GGCATTCACTTGCGCATATG+CGG 0.545602 4_1:-41578802 Msa0547990:five_prime_UTR
CTTCTCTTGATCGGCGATAG+CGG 0.545946 4_1:-41578365 Msa0547990:intron
TAGGCTGTGCCAACGTCCAA+AGG 0.547979 4_1:-41576319 Msa0547990:CDS
AAATTCTTCGAAACTGTAAG+TGG 0.558518 4_1:-41576268 Msa0547990:CDS
CTGTATCTGGCTTCATAAGG+GGG 0.563934 4_1:-41578833 Msa0547990:five_prime_UTR
AATTTGGGATACTGCAGGAC+AGG 0.565679 4_1:-41577153 Msa0547990:CDS
CTCCAAATTTGGGATACTGC+AGG 0.565715 4_1:-41577158 Msa0547990:CDS
CAGCTTCAAAGTACAGTACA+AGG 0.570658 4_1:-41578708 Msa0547990:intron
TTCAAACTAAAGCCACAGAA+AGG 0.577022 4_1:+41576887 None:intergenic
TCGGCACGAGCCCTTGCCGG+TGG 0.580971 4_1:+41578407 None:intergenic
CTGTTTGATTGGGATTAGGA+CGG 0.583469 4_1:-41574834 Msa0547990:three_prime_UTR
TCTGTATCTGGCTTCATAAG+GGG 0.588024 4_1:-41578834 Msa0547990:five_prime_UTR
AGATATGGACGAAAGCAAAA+GGG 0.588273 4_1:-41576699 Msa0547990:intron
GCTTGCTGACACTGACAACA+AGG 0.590249 4_1:-41576107 Msa0547990:CDS
TGCAAAGACAAATCTAAATG+TGG 0.596571 4_1:-41576170 Msa0547990:CDS
AGCTGCCTCTCCTATAATTG+TGG 0.596941 4_1:+41578726 None:intergenic
ATGTTAACAAAATACTGGTA+GGG 0.601571 4_1:-41576730 Msa0547990:CDS
GGGATACTGCAGGACAGGAA+CGG 0.617575 4_1:-41577148 Msa0547990:CDS
CGATCAAGAGAAGCTTAATG+AGG 0.624299 4_1:+41578375 None:intergenic
AAAGACAAATCTAAATGTGG+AGG 0.631704 4_1:-41576167 Msa0547990:CDS
AATCAACCAAGACTCTGCAA+CGG 0.636226 4_1:-41575088 Msa0547990:CDS
TGTGATGAAACTGGTAGTGA+AGG 0.645460 4_1:+41577322 None:intergenic
ATTCACTTGCGCATATGCGG+AGG 0.646954 4_1:-41578799 Msa0547990:five_prime_UTR
TGCTTATCAATGGCGTGTAG+TGG 0.653850 4_1:-41574786 Msa0547990:three_prime_UTR
TAGCTAGAGACATAAAACAA+AGG 0.656013 4_1:-41576129 Msa0547990:CDS
TGACACTGACAACAAGGCAG+AGG 0.656933 4_1:-41576101 Msa0547990:intron
TTATACCACAATTATAGGAG+AGG 0.659889 4_1:-41578731 Msa0547990:five_prime_UTR
ATAGACCAGCAATATGCCCA+TGG 0.666814 4_1:+41576946 None:intergenic
TGTATCTGGCTTCATAAGGG+GGG 0.679081 4_1:-41578832 Msa0547990:five_prime_UTR
TCCTAATCCCAATCAAACAG+AGG 0.688148 4_1:+41574837 None:intergenic
TCAACCAAGACTCTGCAACG+GGG 0.752690 4_1:-41575086 Msa0547990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATGATGAAATTAAAA+AGG + chr4_1:41574859-41574878 None:intergenic 10.0%
!! ACAAAATAAGTTTATATTAA+TGG + chr4_1:41576105-41576124 None:intergenic 10.0%
!!! TTAATTGATTTTGAAATTAA+AGG + chr4_1:41577665-41577684 None:intergenic 10.0%
!! TTAATTTCAAAATCAATTAA+TGG - chr4_1:41577665-41577684 Msa0547990:intron 10.0%
!!! AGTATCTTTTATATATATAT+GGG + chr4_1:41578279-41578298 None:intergenic 10.0%
!!! TAGTATCTTTTATATATATA+TGG + chr4_1:41578280-41578299 None:intergenic 10.0%
!! TTATATTATACCACAATTAT+AGG - chr4_1:41574768-41574787 Msa0547990:three_prime_UTR 15.0%
!! AAAAATTGAAAATGCAAATA+TGG + chr4_1:41575018-41575037 None:intergenic 15.0%
!! TGTTATTGTAATGTAAATTA+TGG - chr4_1:41575369-41575388 Msa0547990:intron 15.0%
!! GTATTAAACAAATAAATTGT+GGG + chr4_1:41575596-41575615 None:intergenic 15.0%
!! TGTATTAAACAAATAAATTG+TGG + chr4_1:41575597-41575616 None:intergenic 15.0%
!! ATTTGTTTAATACACAAATT+AGG - chr4_1:41575603-41575622 Msa0547990:intron 15.0%
!! ATTACACAAAATATATATAG+AGG - chr4_1:41577588-41577607 Msa0547990:intron 15.0%
!! ATTGAATAATAGTAATTGTT+AGG - chr4_1:41577693-41577712 Msa0547990:intron 15.0%
!! TTGAATAATAGTAATTGTTA+GGG - chr4_1:41577694-41577713 Msa0547990:intron 15.0%
!! AAAGAAAAGAATGAAATTAT+TGG + chr4_1:41577909-41577928 None:intergenic 15.0%
!!! AATAATTTCATTCTTTTCTT+TGG - chr4_1:41577908-41577927 Msa0547990:intron 15.0%
!! ATTCAAATTATTAATTCCAT+TGG + chr4_1:41577999-41578018 None:intergenic 15.0%
!!! GTATCTTTTATATATATATG+GGG + chr4_1:41578278-41578297 None:intergenic 15.0%
!!! ATTTTAGAGAGAGAAAATAA+CGG + chr4_1:41574935-41574954 None:intergenic 20.0%
!!! TTCTTTACTTGTGAAAATTT+TGG - chr4_1:41574963-41574982 Msa0547990:three_prime_UTR 20.0%
!! AAAAATGAATCTGTTGAAAA+TGG + chr4_1:41575060-41575079 None:intergenic 20.0%
!! ACCGTATAATAATATAAACT+AGG - chr4_1:41575199-41575218 Msa0547990:intron 20.0%
!! CCCTAGTTTATATTATTATA+CGG + chr4_1:41575203-41575222 None:intergenic 20.0%
!! CCGTATAATAATATAAACTA+GGG - chr4_1:41575200-41575219 Msa0547990:intron 20.0%
!!! GATTTGTTTTAAATTGTGAA+AGG - chr4_1:41575296-41575315 Msa0547990:intron 20.0%
!! CAAACAATGATTCAAATAAT+GGG + chr4_1:41575349-41575368 None:intergenic 20.0%
!! ACAAACAATGATTCAAATAA+TGG + chr4_1:41575350-41575369 None:intergenic 20.0%
!! TTTGTCATTGTCAATAATTT+GGG + chr4_1:41575483-41575502 None:intergenic 20.0%
!! TTAAAATCAATACTGCATTA+AGG + chr4_1:41576289-41576308 None:intergenic 20.0%
!! TAATAAAAAGAAAACCTATG+AGG + chr4_1:41576415-41576434 None:intergenic 20.0%
!!! CTGCAAAAAACAAATATTTT+AGG + chr4_1:41576523-41576542 None:intergenic 20.0%
!!! ATTTGTTATTTATCAACTGT+TGG + chr4_1:41577083-41577102 None:intergenic 20.0%
!! TTCAAAATCAATTAATGGTT+AGG - chr4_1:41577670-41577689 Msa0547990:intron 20.0%
!!! AAATTGATTTTGTCATGATA+AGG + chr4_1:41577775-41577794 None:intergenic 20.0%
!! TGAAATTATTGGAATCTAAA+TGG + chr4_1:41577898-41577917 None:intergenic 20.0%
!! TATACTATGATAATAAGATC+TGG - chr4_1:41578029-41578048 Msa0547990:intron 20.0%
!!! TAAATAAACTTTAAAGCTGT+TGG - chr4_1:41578183-41578202 Msa0547990:intron 20.0%
!! TGTTGGATTAAAAATAATTG+AGG - chr4_1:41578200-41578219 Msa0547990:intron 20.0%
!! GTTGGATTAAAAATAATTGA+GGG - chr4_1:41578201-41578220 Msa0547990:intron 20.0%
!! GTTTATACAGTGAAAAAAAT+GGG + chr4_1:41578697-41578716 None:intergenic 20.0%
!! AGTTTATACAGTGAAAAAAA+TGG + chr4_1:41578698-41578717 None:intergenic 20.0%
!! TTTTCACAAGTAAAGAAACA+TGG + chr4_1:41574960-41574979 None:intergenic 25.0%
!! TTTACTTGTGAAAATTTTGG+AGG - chr4_1:41574966-41574985 Msa0547990:three_prime_UTR 25.0%
!!! GATTCATTTTTTCGTTTTGA+TGG - chr4_1:41575069-41575088 Msa0547990:CDS 25.0%
! ACGGTAAAAAATACATAGAA+GGG + chr4_1:41575184-41575203 None:intergenic 25.0%
! TACGGTAAAAAATACATAGA+AGG + chr4_1:41575185-41575204 None:intergenic 25.0%
!!! TTTTGACCTTGTTTTTGATT+TGG - chr4_1:41575273-41575292 Msa0547990:intron 25.0%
!!! TTTGACCTTGTTTTTGATTT+GGG - chr4_1:41575274-41575293 Msa0547990:intron 25.0%
! ATGTAAATTATGGTATCAAG+AGG - chr4_1:41575379-41575398 Msa0547990:intron 25.0%
! ATTACTGCAATTGTAATGAA+CGG + chr4_1:41575460-41575479 None:intergenic 25.0%
! CTTTGTCATTGTCAATAATT+TGG + chr4_1:41575484-41575503 None:intergenic 25.0%
! AAACTTATAAGCTAAGAGAA+TGG + chr4_1:41575545-41575564 None:intergenic 25.0%
! ATACTTCATTATGTAAACCA+AGG + chr4_1:41576026-41576045 None:intergenic 25.0%
!!! ATTTTGTTATTGTTCTTCAC+AGG - chr4_1:41576118-41576137 Msa0547990:CDS 25.0%
!! AACTATTGGGTATGATTTTA+TGG - chr4_1:41576204-41576223 Msa0547990:CDS 25.0%
!!! TTTTTTTTCTTCCTTTCCAT+GGG - chr4_1:41576677-41576696 Msa0547990:intron 25.0%
! TTCTAATGATTTCAGATATC+AGG - chr4_1:41576714-41576733 Msa0547990:CDS 25.0%
! GGATAATGTTAACAAAATAC+TGG - chr4_1:41576769-41576788 Msa0547990:CDS 25.0%
! AATGTTAACAAAATACTGGT+AGG - chr4_1:41576773-41576792 Msa0547990:intron 25.0%
! ATGTTAACAAAATACTGGTA+GGG - chr4_1:41576774-41576793 Msa0547990:intron 25.0%
!! TAAACATTTTTGTTGACGAA+CGG - chr4_1:41577023-41577042 Msa0547990:intron 25.0%
!! GTACTTTTACCTTTCTTTTT+AGG - chr4_1:41577166-41577185 Msa0547990:CDS 25.0%
! ATACAGAGTCGATATTATAT+AGG - chr4_1:41577547-41577566 Msa0547990:intron 25.0%
! ACACAAAATATATATAGAGG+AGG - chr4_1:41577591-41577610 Msa0547990:intron 25.0%
! CACAAAATATATATAGAGGA+GGG - chr4_1:41577592-41577611 Msa0547990:intron 25.0%
! TAAGTTTATAGACTTACTCT+TGG + chr4_1:41577629-41577648 None:intergenic 25.0%
! TGAAATTAAAGGTCATGATT+TGG + chr4_1:41577654-41577673 None:intergenic 25.0%
!! CTTTTATATATATATGGGGA+GGG + chr4_1:41578274-41578293 None:intergenic 25.0%
!! TCTTTTATATATATATGGGG+AGG + chr4_1:41578275-41578294 None:intergenic 25.0%
! AATATCGTATCGTGTAAATA+AGG + chr4_1:41578304-41578323 None:intergenic 25.0%
!!! AAAAGGTATCACAAAACATT+AGG + chr4_1:41578375-41578394 None:intergenic 25.0%
!! TGTATAAACTTGCTTATCAA+TGG - chr4_1:41578708-41578727 Msa0547990:intron 25.0%
TTATACCACAATTATAGGAG+AGG - chr4_1:41574773-41574792 Msa0547990:three_prime_UTR 30.0%
CTTAATGAGGTAATCGTAAT+CGG + chr4_1:41575119-41575138 None:intergenic 30.0%
GGTAAAAAATACATAGAAGG+GGG + chr4_1:41575182-41575201 None:intergenic 30.0%
CGGTAAAAAATACATAGAAG+GGG + chr4_1:41575183-41575202 None:intergenic 30.0%
CAAATGTTACATAAGAACGA+AGG - chr4_1:41575228-41575247 Msa0547990:intron 30.0%
CAAATCCCAAATCAAAAACA+AGG + chr4_1:41575282-41575301 None:intergenic 30.0%
! TGAAAGGTTTGATCTTTTCA+TGG - chr4_1:41575312-41575331 Msa0547990:intron 30.0%
TAATGAACGGATCACATAAA+AGG + chr4_1:41575447-41575466 None:intergenic 30.0%
GTAATTGAGATCAACTAAAG+GGG + chr4_1:41575742-41575761 None:intergenic 30.0%
GGTAATTGAGATCAACTAAA+GGG + chr4_1:41575743-41575762 None:intergenic 30.0%
AGGTAATTGAGATCAACTAA+AGG + chr4_1:41575744-41575763 None:intergenic 30.0%
ACCTTCTTGTGTAAAATACA+GGG - chr4_1:41575836-41575855 Msa0547990:intron 30.0%
TGTACAATACACTAATTGGT+GGG - chr4_1:41575867-41575886 Msa0547990:intron 30.0%
AGCAACACAGTTATTCAAAT+TGG + chr4_1:41576057-41576076 None:intergenic 30.0%
TGTGATATTAGAGAAATCTC+AGG + chr4_1:41576260-41576279 None:intergenic 30.0%
ATAAGAACCATTGAACTTGA+TGG - chr4_1:41576310-41576329 Msa0547990:CDS 30.0%
!!! GTTTTTTTTCTTCCTTTCCA+TGG - chr4_1:41576676-41576695 Msa0547990:intron 30.0%
! ATCTGAAATCATTAGAAGCT+AGG + chr4_1:41576711-41576730 None:intergenic 30.0%
TGATTTCAGATATCAGGAAT+TGG - chr4_1:41576720-41576739 Msa0547990:CDS 30.0%
! TAAAGGTTGATTTACAGAGA+GGG + chr4_1:41576904-41576923 None:intergenic 30.0%
AGTACATTTGACAACTGTAA+AGG + chr4_1:41576921-41576940 None:intergenic 30.0%
GTAAATTTGCATGACGATTA+TGG + chr4_1:41576945-41576964 None:intergenic 30.0%
TAATCGTCATGCAAATTTAC+TGG - chr4_1:41576945-41576964 Msa0547990:CDS 30.0%
AAGGTTGAATTGCATTGTTA+AGG - chr4_1:41576968-41576987 Msa0547990:intron 30.0%
! AAACATTTTTGTTGACGAAC+GGG - chr4_1:41577024-41577043 Msa0547990:intron 30.0%
AAATTCTTCGAAACTGTAAG+TGG - chr4_1:41577236-41577255 Msa0547990:intron 30.0%
! GTGTATTTTACCTGAAAATG+TGG - chr4_1:41577258-41577277 Msa0547990:intron 30.0%
TGCAAAGACAAATCTAAATG+TGG - chr4_1:41577334-41577353 Msa0547990:CDS 30.0%
AAAGACAAATCTAAATGTGG+AGG - chr4_1:41577337-41577356 Msa0547990:CDS 30.0%
!! TTGTTTTATGTCTCTAGCTA+TGG + chr4_1:41577375-41577394 None:intergenic 30.0%
TAGCTAGAGACATAAAACAA+AGG - chr4_1:41577375-41577394 Msa0547990:intron 30.0%
TTAGAGACATGAAACTATGA+CGG + chr4_1:41577465-41577484 None:intergenic 30.0%
TAATAGTAATTGTTAGGGTG+AGG - chr4_1:41577699-41577718 Msa0547990:intron 30.0%
GATCTCAAAATGTACTTCTT+TGG - chr4_1:41577864-41577883 Msa0547990:intron 30.0%
TTAGGTAATCCCAATTTAAG+AGG - chr4_1:41577946-41577965 Msa0547990:intron 30.0%
!!! TGATTTGTTTTTGTCGTTTG+CGG - chr4_1:41578100-41578119 Msa0547990:intron 30.0%
! ATTTTGTTGAGTGTGTTTAG+AGG - chr4_1:41578135-41578154 Msa0547990:intron 30.0%
CTCATTAAGCTTCTCTTGAT+CGG - chr4_1:41575130-41575149 Msa0547990:intron 35.0%
TACACAAATTAGGCCTTAAG+CGG - chr4_1:41575613-41575632 Msa0547990:intron 35.0%
TGGATCGAATTCTTACCAAA+TGG + chr4_1:41575668-41575687 None:intergenic 35.0%
ACTAGTAGTCTAGTACGTAT+AGG - chr4_1:41575690-41575709 Msa0547990:intron 35.0%
CACCTTCTTGTGTAAAATAC+AGG - chr4_1:41575835-41575854 Msa0547990:intron 35.0%
! ACCCTGTATTTTACACAAGA+AGG + chr4_1:41575840-41575859 None:intergenic 35.0%
CTTGTGTAAAATACAGGGTA+AGG - chr4_1:41575841-41575860 Msa0547990:intron 35.0%
GCTGTGTACAATACACTAAT+TGG - chr4_1:41575863-41575882 Msa0547990:intron 35.0%
GTGTACAATACACTAATTGG+TGG - chr4_1:41575866-41575885 Msa0547990:intron 35.0%
GTAATTGAGATCACCTAAAG+GGG + chr4_1:41575954-41575973 None:intergenic 35.0%
GGTAATTGAGATCACCTAAA+GGG + chr4_1:41575955-41575974 None:intergenic 35.0%
AGGTAATTGAGATCACCTAA+AGG + chr4_1:41575956-41575975 None:intergenic 35.0%
! GCTTTTGTGTGAAACATACT+TGG - chr4_1:41576074-41576093 Msa0547990:intron 35.0%
TTATTGTTCTTCACAGGTGT+TGG - chr4_1:41576124-41576143 Msa0547990:CDS 35.0%
! CTTCTTTTGCGTTTTTCTGA+TGG - chr4_1:41576157-41576176 Msa0547990:CDS 35.0%
ACCAGTTTCATCACAACTAT+TGG - chr4_1:41576190-41576209 Msa0547990:CDS 35.0%
CCAGTTTCATCACAACTATT+GGG - chr4_1:41576191-41576210 Msa0547990:CDS 35.0%
AACGTATCAAGCTCCAAATT+TGG - chr4_1:41576335-41576354 Msa0547990:intron 35.0%
ACGTATCAAGCTCCAAATTT+GGG - chr4_1:41576336-41576355 Msa0547990:intron 35.0%
GTAAGGTTTATGATCCTCAT+AGG - chr4_1:41576398-41576417 Msa0547990:intron 35.0%
!!! TTTTTTGCAGCTTACTATCG+TGG - chr4_1:41576533-41576552 Msa0547990:intron 35.0%
AACAGTAATGTTCCTTTCTG+TGG - chr4_1:41576605-41576624 Msa0547990:intron 35.0%
TTCAAACTAAAGCCACAGAA+AGG + chr4_1:41576620-41576639 None:intergenic 35.0%
AGATATGGACGAAAGCAAAA+GGG - chr4_1:41576805-41576824 Msa0547990:intron 35.0%
! AAAGGGTATGCAAACTTTGT+TGG - chr4_1:41576822-41576841 Msa0547990:intron 35.0%
! AAAGGTTGATTTACAGAGAG+GGG + chr4_1:41576903-41576922 None:intergenic 35.0%
! GTAAAGGTTGATTTACAGAG+AGG + chr4_1:41576905-41576924 None:intergenic 35.0%
! AACATTTTTGTTGACGAACG+GGG - chr4_1:41577025-41577044 Msa0547990:intron 35.0%
TAGAGACATGAAACTATGAC+GGG + chr4_1:41577464-41577483 None:intergenic 35.0%
GAGAGAAAAGTAGAAAGACA+TGG - chr4_1:41577506-41577525 Msa0547990:intron 35.0%
GACTCTGTATTTCGAAAACA+CGG + chr4_1:41577537-41577556 None:intergenic 35.0%
! GGTTTAATTGTTTTCTCCAC+TGG - chr4_1:41577720-41577739 Msa0547990:intron 35.0%
AGCATCTCACCTCTTAAATT+GGG + chr4_1:41577958-41577977 None:intergenic 35.0%
AAGCATCTCACCTCTTAAAT+TGG + chr4_1:41577959-41577978 None:intergenic 35.0%
AATTTAAGAGGTGAGATGCT+TGG - chr4_1:41577958-41577977 Msa0547990:intron 35.0%
ATTTAAGAGGTGAGATGCTT+GGG - chr4_1:41577959-41577978 Msa0547990:intron 35.0%
GGGATAATTATTGTAGCCAA+TGG - chr4_1:41577980-41577999 Msa0547990:intron 35.0%
AATAATTGAGGGCTGAGATT+TGG - chr4_1:41578212-41578231 Msa0547990:intron 35.0%
TAAGTTTAGAGACCTACTCT+TGG - chr4_1:41578237-41578256 Msa0547990:intron 35.0%
!!! GATATTTTGACTCCAAGAGT+AGG + chr4_1:41577614-41577633 None:intergenic 35.0%
!! TGGTTGATTTTGATGGTTGT+TGG + chr4_1:41578405-41578424 None:intergenic 35.0%
!! AGAGTCTTGGTTGATTTTGA+TGG + chr4_1:41578412-41578431 None:intergenic 35.0%
! TTGAGGACAGTTTTACAAGT+TGG - chr4_1:41578476-41578495 Msa0547990:intron 35.0%
! TTGTGCGTGATAAGTAGTTT+TGG - chr4_1:41578590-41578609 Msa0547990:intron 35.0%
TCTAACTACCTCTGTTTGAT+TGG - chr4_1:41578659-41578678 Msa0547990:intron 35.0%
CTAACTACCTCTGTTTGATT+GGG - chr4_1:41578660-41578679 Msa0547990:intron 35.0%
! AGTCTGTATCTGGCTTCATA+AGG - chr4_1:41574668-41574687 Msa0547990:three_prime_UTR 40.0%
! GTCTGTATCTGGCTTCATAA+GGG - chr4_1:41574669-41574688 Msa0547990:three_prime_UTR 40.0%
! TCTGTATCTGGCTTCATAAG+GGG - chr4_1:41574670-41574689 Msa0547990:three_prime_UTR 40.0%
CAGCTTCAAAGTACAGTACA+AGG - chr4_1:41574796-41574815 Msa0547990:three_prime_UTR 40.0%
CGATCAAGAGAAGCTTAATG+AGG + chr4_1:41575132-41575151 None:intergenic 40.0%
GTTTCGGTCTCTTAACCATT+TGG - chr4_1:41575650-41575669 Msa0547990:intron 40.0%
ACGTACTAGACTACTAGTCA+TGG + chr4_1:41575688-41575707 None:intergenic 40.0%
TTGATCTCAATTACCTCGAG+TGG - chr4_1:41575748-41575767 Msa0547990:intron 40.0%
!! TTACACAAGAAGGTGTTTCC+AGG + chr4_1:41575830-41575849 None:intergenic 40.0%
! TGTGATGAAACTGGTAGTGA+AGG + chr4_1:41576185-41576204 None:intergenic 40.0%
CCCAATAGTTGTGATGAAAC+TGG + chr4_1:41576194-41576213 None:intergenic 40.0%
ACGTTTGCCATCAAGTTCAA+TGG + chr4_1:41576320-41576339 None:intergenic 40.0%
CCTTACCTGTTGTAATTGTC+CGG + chr4_1:41576384-41576403 None:intergenic 40.0%
CAACATTGAGCAACATGCTT+CGG - chr4_1:41576748-41576767 Msa0547990:CDS 40.0%
CAGATATGGACGAAAGCAAA+AGG - chr4_1:41576804-41576823 Msa0547990:intron 40.0%
! AAGGTTGATTTACAGAGAGG+GGG + chr4_1:41576902-41576921 None:intergenic 40.0%
CGTCATGCAAATTTACTGGA+AGG - chr4_1:41576949-41576968 Msa0547990:CDS 40.0%
TTAGTTCACTCACAGCAACA+TGG + chr4_1:41577006-41577025 None:intergenic 40.0%
! AAGAGGGTTGAGTTCTGATT+TGG - chr4_1:41577055-41577074 Msa0547990:intron 40.0%
TTGGCACAGCCTAAAAAGAA+AGG + chr4_1:41577178-41577197 None:intergenic 40.0%
! CAGAGAGTTTCCACATTTTC+AGG + chr4_1:41577271-41577290 None:intergenic 40.0%
CTCATTCAACATGAGAGAAG+AGG + chr4_1:41577489-41577508 None:intergenic 40.0%
TGGATGAACGCAGAATTCTT+AGG - chr4_1:41577928-41577947 Msa0547990:intron 40.0%
TTTAAGAGGTGAGATGCTTG+GGG - chr4_1:41577960-41577979 Msa0547990:intron 40.0%
TGTTGAGTGTGTTTAGAGGA+AGG - chr4_1:41578139-41578158 Msa0547990:intron 40.0%
AACATTAGGCTCGTGAGAAA+GGG + chr4_1:41578361-41578380 None:intergenic 40.0%
AAACATTAGGCTCGTGAGAA+AGG + chr4_1:41578362-41578381 None:intergenic 40.0%
! TGGTTGTTGGCTGCAATAAA+AGG + chr4_1:41578392-41578411 None:intergenic 40.0%
AATCAACCAAGACTCTGCAA+CGG - chr4_1:41578416-41578435 Msa0547990:CDS 40.0%
! TGTGCTCAACGTTTTCAATG+TGG - chr4_1:41578552-41578571 Msa0547990:intron 40.0%
ACCTCTGTTTGATTGGGATT+AGG - chr4_1:41578666-41578685 Msa0547990:intron 40.0%
TCCTAATCCCAATCAAACAG+AGG + chr4_1:41578670-41578689 None:intergenic 40.0%
CTGTTTGATTGGGATTAGGA+CGG - chr4_1:41578670-41578689 Msa0547990:intron 40.0%
! CTATGCGTTGATAGATGCTT+CGG - chr4_1:41578789-41578808 Msa0547990:five_prime_UTR 40.0%
! CTGTATCTGGCTTCATAAGG+GGG - chr4_1:41574671-41574690 Msa0547990:three_prime_UTR 45.0%
! TGTATCTGGCTTCATAAGGG+GGG - chr4_1:41574672-41574691 Msa0547990:three_prime_UTR 45.0%
AGCTGCCTCTCCTATAATTG+TGG + chr4_1:41574781-41574800 None:intergenic 45.0%
GTGAGAGAAAGAGAGAGAAG+TGG + chr4_1:41574996-41575015 None:intergenic 45.0%
GGTCCAAGTGGTAGATGTTT+CGG - chr4_1:41575634-41575653 Msa0547990:intron 45.0%
AGACCGAAACATCTACCACT+TGG + chr4_1:41575640-41575659 None:intergenic 45.0%
CCTAAAGGGGCTGACATAAA+GGG + chr4_1:41575941-41575960 None:intergenic 45.0%
ACCTAAAGGGGCTGACATAA+AGG + chr4_1:41575942-41575961 None:intergenic 45.0%
GTGATCTCAATTACCTCGAG+TGG - chr4_1:41575960-41575979 Msa0547990:intron 45.0%
! CTCCAAATTTGGGATACTGC+AGG - chr4_1:41576346-41576365 Msa0547990:intron 45.0%
GTCCTGCAGTATCCCAAATT+TGG + chr4_1:41576351-41576370 None:intergenic 45.0%
! AATTTGGGATACTGCAGGAC+AGG - chr4_1:41576351-41576370 Msa0547990:intron 45.0%
CCGGACAATTACAACAGGTA+AGG - chr4_1:41576381-41576400 Msa0547990:intron 45.0%
GAAACACGCTGTTTGACTGT+TGG - chr4_1:41576468-41576487 Msa0547990:intron 45.0%
ATAGACCAGCAATATGCCCA+TGG + chr4_1:41576561-41576580 None:intergenic 45.0%
AGGTGAATACTCCCATGGAA+AGG + chr4_1:41576691-41576710 None:intergenic 45.0%
AAGCTAGGTGAATACTCCCA+TGG + chr4_1:41576696-41576715 None:intergenic 45.0%
GGTAGGGAACAAAGCAGATA+TGG - chr4_1:41576790-41576809 Msa0547990:intron 45.0%
! AGGTTGATTTACAGAGAGGG+GGG + chr4_1:41576901-41576920 None:intergenic 45.0%
! ATTTTTGTTGACGAACGGGG+AGG - chr4_1:41577028-41577047 Msa0547990:intron 45.0%
! TTGCAGCAACAAGCGTTTTG+CGG + chr4_1:41577750-41577769 None:intergenic 45.0%
!! TGTTTTTGTCGTTTGCGGCA+AGG - chr4_1:41578105-41578124 Msa0547990:intron 45.0%
!! GTTTTTGTCGTTTGCGGCAA+GGG - chr4_1:41578106-41578125 Msa0547990:intron 45.0%
GTGTGTTTAGAGGAAGGAAG+CGG - chr4_1:41578145-41578164 Msa0547990:intron 45.0%
ATCAACCAAGACTCTGCAAC+GGG - chr4_1:41578417-41578436 Msa0547990:CDS 45.0%
! GCACAAAAATCTGCTTGCTG+TGG - chr4_1:41578453-41578472 Msa0547990:five_prime_UTR 45.0%
!! AAATCTGCTTGCTGTGGTTG+AGG - chr4_1:41578459-41578478 Msa0547990:five_prime_UTR 45.0%
TGCTTATCAATGGCGTGTAG+TGG - chr4_1:41578718-41578737 Msa0547990:five_prime_UTR 45.0%
GGCATTCACTTGCGCATATG+CGG - chr4_1:41574702-41574721 Msa0547990:three_prime_UTR 50.0%
ATTCACTTGCGCATATGCGG+AGG - chr4_1:41574705-41574724 Msa0547990:three_prime_UTR 50.0%
ACAGAGAGAGAGAGAGAGAG+AGG + chr4_1:41574836-41574855 None:intergenic 50.0%
CTTCTCTTGATCGGCGATAG+CGG - chr4_1:41575139-41575158 Msa0547990:intron 50.0%
TCTACCACTTGGACCGCTTA+AGG + chr4_1:41575629-41575648 None:intergenic 50.0%
CTTTACTGTTGCACCACTCG+AGG + chr4_1:41575764-41575783 None:intergenic 50.0%
TCGCTTGTCACGTGACTGAA+AGG - chr4_1:41575790-41575809 Msa0547990:intron 50.0%
AAGGTCACGAGTTCAAGTCC+TGG - chr4_1:41575809-41575828 Msa0547990:intron 50.0%
CACTAAAGCTCCCGCATACA+CGG + chr4_1:41575908-41575927 None:intergenic 50.0%
! ATGCGGGAGCTTTAGTGCAT+CGG - chr4_1:41575911-41575930 Msa0547990:intron 50.0%
CCCTTTATGTCAGCCCCTTT+AGG - chr4_1:41575938-41575957 Msa0547990:intron 50.0%
CGGTTCCGGACAATTACAAC+AGG - chr4_1:41576376-41576395 Msa0547990:intron 50.0%
GCTGTTTGACTGTTGGCATC+TGG - chr4_1:41576475-41576494 Msa0547990:intron 50.0%
! AGCTTACTATCGTGGTGCCA+TGG - chr4_1:41576541-41576560 Msa0547990:intron 50.0%
!! GCTTACTATCGTGGTGCCAT+GGG - chr4_1:41576542-41576561 Msa0547990:intron 50.0%
TTGATTTACAGAGAGGGGGG+GGG + chr4_1:41576898-41576917 None:intergenic 50.0%
! GTTGATTTACAGAGAGGGGG+GGG + chr4_1:41576899-41576918 None:intergenic 50.0%
! GGTTGATTTACAGAGAGGGG+GGG + chr4_1:41576900-41576919 None:intergenic 50.0%
ACACACACATACGAGAGAGG+AGG + chr4_1:41577111-41577130 None:intergenic 50.0%
GACACACACACATACGAGAG+AGG + chr4_1:41577114-41577133 None:intergenic 50.0%
CATACTCATCAGCCAGTGCT+TGG + chr4_1:41577211-41577230 None:intergenic 50.0%
!! CAAGCACTGGCTGATGAGTA+TGG - chr4_1:41577209-41577228 Msa0547990:CDS 50.0%
! GCTTGCTGACACTGACAACA+AGG - chr4_1:41577397-41577416 Msa0547990:intron 50.0%
TGACACTGACAACAAGGCAG+AGG - chr4_1:41577403-41577422 Msa0547990:intron 50.0%
! AGCGTTTTGCGGTATTCCAG+TGG + chr4_1:41577739-41577758 None:intergenic 50.0%
AGAGGAAGGAAGCGGAAAAG+TGG - chr4_1:41578153-41578172 Msa0547990:intron 50.0%
TCAACCAAGACTCTGCAACG+GGG - chr4_1:41578418-41578437 Msa0547990:CDS 50.0%
CTTCATAAGGGGGGAGTTGC+TGG - chr4_1:41574681-41574700 Msa0547990:three_prime_UTR 55.0%
!! CGTTTTGATGGCTGCTCCAC+CGG - chr4_1:41575081-41575100 Msa0547990:CDS 55.0%
TAGGCCTTAAGCGGTCCAAG+TGG - chr4_1:41575622-41575641 Msa0547990:intron 55.0%
! TGCGGGAGCTTTAGTGCATC+GGG - chr4_1:41575912-41575931 Msa0547990:intron 55.0%
GCAGACACACTAACCACTCG+AGG + chr4_1:41575976-41575995 None:intergenic 55.0%
CGTTGCACGCTGAGATACCT+TGG - chr4_1:41576006-41576025 Msa0547990:intron 55.0%
GGGATACTGCAGGACAGGAA+CGG - chr4_1:41576356-41576375 Msa0547990:intron 55.0%
!! TGGTGCCATGGGCATATTGC+TGG - chr4_1:41576553-41576572 Msa0547990:intron 55.0%
TGATTTACAGAGAGGGGGGG+GGG + chr4_1:41576897-41576916 None:intergenic 55.0%
TAGGCTGTGCCAACGTCCAA+AGG - chr4_1:41577185-41577204 Msa0547990:CDS 55.0%
! AGTGCTTGGCCTTTGGACGT+TGG + chr4_1:41577197-41577216 None:intergenic 55.0%
AACGTCCAAAGGCCAAGCAC+TGG - chr4_1:41577196-41577215 Msa0547990:CDS 55.0%
! ATCAGCCAGTGCTTGGCCTT+TGG + chr4_1:41577204-41577223 None:intergenic 55.0%
TAATCGGCACGAGCCCTTGC+CGG + chr4_1:41575103-41575122 None:intergenic 60.0%
CATACACGGCGTCTGGGAAG+GGG + chr4_1:41575894-41575913 None:intergenic 60.0%
GCATACACGGCGTCTGGGAA+GGG + chr4_1:41575895-41575914 None:intergenic 60.0%
CTTCCCAGACGCCGTGTATG+CGG - chr4_1:41575894-41575913 Msa0547990:intron 60.0%
TTCCCAGACGCCGTGTATGC+GGG - chr4_1:41575895-41575914 Msa0547990:intron 60.0%
CTGCAGGACAGGAACGGTTC+CGG - chr4_1:41576362-41576381 Msa0547990:intron 60.0%
GGGGGAACGAAAAAGGCAGG+AGG + chr4_1:41576879-41576898 None:intergenic 60.0%
ACGAACGGGGAGGTTCGAAG+AGG - chr4_1:41577038-41577057 Msa0547990:intron 60.0%
CGAACGGGGAGGTTCGAAGA+GGG - chr4_1:41577039-41577058 Msa0547990:intron 60.0%
CAGACCCCGTTGCAGAGTCT+TGG + chr4_1:41578425-41578444 None:intergenic 60.0%
CAAGACTCTGCAACGGGGTC+TGG - chr4_1:41578423-41578442 Msa0547990:exon 60.0%
TGATGGCTGCTCCACCGGCA+AGG - chr4_1:41575086-41575105 Msa0547990:CDS 65.0%
GATGGCTGCTCCACCGGCAA+GGG - chr4_1:41575087-41575106 Msa0547990:CDS 65.0%
CGCATACACGGCGTCTGGGA+AGG + chr4_1:41575896-41575915 None:intergenic 65.0%
CTCCCGCATACACGGCGTCT+GGG + chr4_1:41575900-41575919 None:intergenic 65.0%
GGGGGGGGAACGAAAAAGGC+AGG + chr4_1:41576882-41576901 None:intergenic 65.0%
GAGGGGGGGGGGAACGAAAA+AGG + chr4_1:41576886-41576905 None:intergenic 65.0%
CTCTGCAACGGGGTCTGGAC+AGG - chr4_1:41578428-41578447 Msa0547990:exon 65.0%
GCTCCCGCATACACGGCGTC+TGG + chr4_1:41575901-41575920 None:intergenic 70.0%
TCGGCACGAGCCCTTGCCGG+TGG + chr4_1:41575100-41575119 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 41574666 41578860 41574666 ID=Msa0547990;Name=Msa0547990
chr4_1 mRNA 41574666 41578860 41574666 ID=Msa0547990-mRNA-1;Parent=Msa0547990;Name=Msa0547990-mRNA-1;_AED=0.12;_eAED=0.12;_QI=197|0.85|0.62|1|0.85|0.75|8|381|253
chr4_1 exon 41574666 41575124 41574666 ID=Msa0547990-mRNA-1:exon:10701;Parent=Msa0547990-mRNA-1
chr4_1 exon 41576102 41576338 41576102 ID=Msa0547990-mRNA-1:exon:10700;Parent=Msa0547990-mRNA-1
chr4_1 exon 41576700 41576790 41576700 ID=Msa0547990-mRNA-1:exon:10699;Parent=Msa0547990-mRNA-1
chr4_1 exon 41576875 41576983 41576875 ID=Msa0547990-mRNA-1:exon:10698;Parent=Msa0547990-mRNA-1
chr4_1 exon 41577129 41577229 41577129 ID=Msa0547990-mRNA-1:exon:10697;Parent=Msa0547990-mRNA-1
chr4_1 exon 41577314 41577386 41577314 ID=Msa0547990-mRNA-1:exon:10696;Parent=Msa0547990-mRNA-1
chr4_1 exon 41578366 41578483 41578366 ID=Msa0547990-mRNA-1:exon:10695;Parent=Msa0547990-mRNA-1
chr4_1 exon 41578709 41578860 41578709 ID=Msa0547990-mRNA-1:exon:10694;Parent=Msa0547990-mRNA-1
chr4_1 five_prime_UTR 41578709 41578860 41578709 ID=Msa0547990-mRNA-1:five_prime_utr;Parent=Msa0547990-mRNA-1
chr4_1 five_prime_UTR 41578439 41578483 41578439 ID=Msa0547990-mRNA-1:five_prime_utr;Parent=Msa0547990-mRNA-1
chr4_1 CDS 41578366 41578438 41578366 ID=Msa0547990-mRNA-1:cds;Parent=Msa0547990-mRNA-1
chr4_1 CDS 41577314 41577386 41577314 ID=Msa0547990-mRNA-1:cds;Parent=Msa0547990-mRNA-1
chr4_1 CDS 41577129 41577229 41577129 ID=Msa0547990-mRNA-1:cds;Parent=Msa0547990-mRNA-1
chr4_1 CDS 41576875 41576983 41576875 ID=Msa0547990-mRNA-1:cds;Parent=Msa0547990-mRNA-1
chr4_1 CDS 41576700 41576790 41576700 ID=Msa0547990-mRNA-1:cds;Parent=Msa0547990-mRNA-1
chr4_1 CDS 41576102 41576338 41576102 ID=Msa0547990-mRNA-1:cds;Parent=Msa0547990-mRNA-1
chr4_1 CDS 41575047 41575124 41575047 ID=Msa0547990-mRNA-1:cds;Parent=Msa0547990-mRNA-1
chr4_1 three_prime_UTR 41574666 41575046 41574666 ID=Msa0547990-mRNA-1:three_prime_utr;Parent=Msa0547990-mRNA-1
Gene Sequence

>Msa0547990

ATGGCTGCTCCACCGGCAAGGGCTCGTGCCGATTACGATTACCTCATTAAGCTTCTCTTGATCGGCGATAGCGGTGTTGGAAAAAGTTGTCTTCTTTTGCGTTTTTCTGATGGTTCCTTCACTACCAGTTTCATCACAACTATTGGTATTGATTTTAAGATAAGAACCATTGAACTTGATGGCAAACGTATCAAGCTCCAAATTTGGGATACTGCAGGACAGGAACGGTTCCGGACAATTACAACAGCTTACTATCGTGGTGCCATGGGCATATTGCTGGTCTATGATGTTACTGATGAAGCATCATTTAACAGTAATGTTCCTTTCTGTGGCTTTAGTTTGAAATCTGCTGATCTGAATTGGATTCGCAACATTGAGCAACATGCTTCGGATAATGTTAACAAAATACTGGTAGGGAACAAAGCAGATATGGACGAAAGCAAAAGGGCTGTGCCAACGTCCAAAGGCCAAGCACTGGCTGATGAGTATGGAATCAAATTCTTCGAAACTGTAAGTGGTGTATTTTACCTGAAAATGTGGAAACTCTCTGAACTTCTATGTTGTTATGCTCATGAAAATGCTCAATTGCAGAGTGCAAAGACAAATCTAAATGTGGAGGAAGTTTTCTTTTCCATAGCTAGAGACATAAAACAAAGGCTTGCTGACACTGACAACAAGGCAGAGCCAACAACCATCAAAATCAACCAAGACTCTGCAACGGGGTCTGGACAGGCTGCACAAAAATCTGCTTGCTGTGGTTGA
Protein sequence

>Msa0547990

MAAPPARARADYDYLIKLLLIGDSGVGKSCLLLRFSDGSFTTSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDEASFNSNVPFCGFSLKSADLNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETVSGVFYLKMWKLSELLCCYAHENAQLQSAKTNLNVEEVFFSIARDIKQRLADTDNKAEPTTIKINQDSATGSGQAAQKSACCG*