AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0562280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTATCAAATGGGGAATT+TGG 0.130501 4_1:-61964174 None:intergenic
TACTTTGTATTAATGCAAAA+TGG 0.251909 4_1:-61964225 None:intergenic
ATCTATCAAATGGGGAATTT+GGG 0.276524 4_1:-61964173 None:intergenic
CAAATTCCCCATTTGATAGA+TGG 0.294471 4_1:+61964175 Msa0562280:CDS
GCCTGCCGACATAAATGTTT+GGG 0.347784 4_1:+61961661 None:intergenic
GGCCTGCCGACATAAATGTT+TGG 0.373077 4_1:+61961660 None:intergenic
CAATTAAGCCATCTATCAAA+TGG 0.384545 4_1:-61964183 None:intergenic
AATTAAGCCATCTATCAAAT+GGG 0.415313 4_1:-61964182 None:intergenic
ATTTGAATTTAAGTGCAAGT+TGG 0.495066 4_1:-61964147 None:intergenic
AAGTGCAAGTTGGTAAGAGT+TGG 0.500362 4_1:-61964137 None:intergenic
TAGATGGAGGGAATTCTTCA+AGG 0.508083 4_1:+61963174 Msa0562280:intron
GAGACAAATGCTCAAAGCTC+AGG 0.519278 4_1:+61963227 Msa0562280:CDS
CGTTTATCATTTCTGCAACA+AGG 0.531767 4_1:+61964106 Msa0562280:CDS
TCTATCAAATGGGGAATTTG+GGG 0.567067 4_1:-61964172 None:intergenic
ATAAACGTTGATTTCCCACC+AGG 0.567312 4_1:-61964090 None:intergenic
CGTTGTTGCATGTAGATGGA+GGG 0.589194 4_1:+61963162 Msa0562280:intron
ATTAAGCCATCTATCAAATG+GGG 0.635793 4_1:-61964181 None:intergenic
CTATCAAATGGGGAATTTGG+GGG 0.649875 4_1:-61964171 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTTAATAATCATAACA+AGG + chr4_1:61962562-61962581 Msa0562280:intron 10.0%
!! TTATTTAATAATCATAACAA+GGG + chr4_1:61962563-61962582 Msa0562280:intron 10.0%
!!! TTGTAAAATTGATTTATATT+TGG - chr4_1:61962943-61962962 None:intergenic 10.0%
!! TGTATAAAATATATCTACTT+TGG + chr4_1:61962340-61962359 Msa0562280:intron 15.0%
!!! TACATTTTCTTTTCTTTTTT+CGG - chr4_1:61962638-61962657 None:intergenic 15.0%
!!! AACAAAAGTATATTTCAAAA+CGG - chr4_1:61963374-61963393 None:intergenic 15.0%
!!! TTTTGAAATATACTTTTGTT+TGG + chr4_1:61963374-61963393 Msa0562280:intron 15.0%
!!! ATTTTAATTTCAAGAAACAA+AGG + chr4_1:61963570-61963589 Msa0562280:intron 15.0%
!! AGCAATTATAAAAATGTAAA+AGG - chr4_1:61963692-61963711 None:intergenic 15.0%
!! AAATAGTAGATTAAAACATA+CGG - chr4_1:61964061-61964080 None:intergenic 15.0%
!!! ATTTTAACTAGCTAATAAGA+TGG - chr4_1:61962100-61962119 None:intergenic 20.0%
!! AAAATGTTTCACAAAGTAAA+CGG + chr4_1:61962148-61962167 Msa0562280:intron 20.0%
!! TATGTTTACAAGTGAATAAT+TGG + chr4_1:61962189-61962208 Msa0562280:intron 20.0%
!!! GGTTATTTAAGATAAGTTAT+TGG + chr4_1:61962361-61962380 Msa0562280:intron 20.0%
!! TTAAAACCGTTAAAAGATAT+AGG - chr4_1:61962459-61962478 None:intergenic 20.0%
!!! AAATTTGATTTTGACATGTT+TGG + chr4_1:61962805-61962824 Msa0562280:intron 20.0%
!! TAAAGAAGAAATTGAAAGTT+TGG - chr4_1:61963028-61963047 None:intergenic 20.0%
!! AAGTTTATTTGTCAAAACAT+TGG + chr4_1:61963475-61963494 Msa0562280:intron 20.0%
!! ATAACTATATTAAGTCAACA+AGG + chr4_1:61963512-61963531 Msa0562280:intron 20.0%
!! TAACTATATTAAGTCAACAA+GGG + chr4_1:61963513-61963532 Msa0562280:intron 20.0%
!! AAATAAAGGATGTGAATAAA+TGG + chr4_1:61963624-61963643 Msa0562280:intron 20.0%
!!! GAATTAAAGAAAGCAATTTT+TGG - chr4_1:61963728-61963747 None:intergenic 20.0%
!! AATCAATATTGTTTAGACTT+TGG + chr4_1:61963937-61963956 Msa0562280:intron 20.0%
!!! CAAAATGGATTTTTTTCTTT+GGG - chr4_1:61964213-61964232 None:intergenic 20.0%
!! TACTTTGTATTAATGCAAAA+TGG - chr4_1:61964228-61964247 None:intergenic 20.0%
! TAGTGAGTTCATTAATTTAG+AGG + chr4_1:61961844-61961863 Msa0562280:intron 25.0%
!! GATAATGAAAACAATGCTTT+TGG - chr4_1:61962007-61962026 None:intergenic 25.0%
!! AATTAGGGGTTATGTATTTT+TGG - chr4_1:61962031-61962050 None:intergenic 25.0%
! AGAAAGTGAAATGCTAATTA+GGG - chr4_1:61962046-61962065 None:intergenic 25.0%
! AAATGTTTCACAAAGTAAAC+GGG + chr4_1:61962149-61962168 Msa0562280:intron 25.0%
! TTTACAAGTGAATAATTGGT+AGG + chr4_1:61962193-61962212 Msa0562280:intron 25.0%
!! GTTTGACCTATATCTTTTAA+CGG + chr4_1:61962450-61962469 Msa0562280:intron 25.0%
!! GATAAAATTCAAAGAGTATG+AGG - chr4_1:61963064-61963083 None:intergenic 25.0%
! CATAATCCAATTAGAGAAAA+GGG - chr4_1:61963112-61963131 None:intergenic 25.0%
!! CTTTTCTCTAATTGGATTAT+GGG + chr4_1:61963111-61963130 Msa0562280:intron 25.0%
!! TTTTCTCTAATTGGATTATG+GGG + chr4_1:61963112-61963131 Msa0562280:intron 25.0%
!! CAAAATTTTACAGCAGAATT+TGG - chr4_1:61963331-61963350 None:intergenic 25.0%
!! AATTTTGCCGGTAAAATATT+AGG + chr4_1:61963344-61963363 Msa0562280:intron 25.0%
! AATTCTATAGTGCACAAATT+AGG + chr4_1:61963743-61963762 Msa0562280:intron 25.0%
!!! GTAAAATTGATTTGATTCAG+CGG - chr4_1:61963801-61963820 None:intergenic 25.0%
! ATTTGAATTTAAGTGCAAGT+TGG - chr4_1:61964150-61964169 None:intergenic 25.0%
! AATTAAGCCATCTATCAAAT+GGG - chr4_1:61964185-61964204 None:intergenic 25.0%
!!! GCAAAATGGATTTTTTTCTT+TGG - chr4_1:61964214-61964233 None:intergenic 25.0%
GATTCCAAATATTCAAACAG+AGG - chr4_1:61961755-61961774 None:intergenic 30.0%
ACTCACTATAGTATAAAGAG+AGG - chr4_1:61961832-61961851 None:intergenic 30.0%
! GAAACGTTTTCACAAAGTTT+AGG - chr4_1:61961878-61961897 None:intergenic 30.0%
GAAAGTGAAATGCTAATTAG+GGG - chr4_1:61962045-61962064 None:intergenic 30.0%
GAGAAAGTGAAATGCTAATT+AGG - chr4_1:61962047-61962066 None:intergenic 30.0%
TTAACTAGCTAATAAGATGG+AGG - chr4_1:61962097-61962116 None:intergenic 30.0%
TAGCAGTTGAAGAAAACATA+TGG + chr4_1:61962222-61962241 Msa0562280:intron 30.0%
!! TCAGTTGTAGAACTTGTTTT+AGG + chr4_1:61962252-61962271 Msa0562280:intron 30.0%
!!! TAGAACTTGTTTTAGGAGAT+TGG + chr4_1:61962259-61962278 Msa0562280:intron 30.0%
ACAAGAAGGAATTGTAACAT+AGG - chr4_1:61962432-61962451 None:intergenic 30.0%
AAGATATAGGTCAAACAAGA+AGG - chr4_1:61962446-61962465 None:intergenic 30.0%
TATGTTCAAGTCATGTCTTA+AGG + chr4_1:61962517-61962536 Msa0562280:intron 30.0%
ATGTTCAAGTCATGTCTTAA+GGG + chr4_1:61962518-61962537 Msa0562280:intron 30.0%
CTAAATATTACGTGCATGTT+TGG - chr4_1:61962988-61963007 None:intergenic 30.0%
CCATAATCCAATTAGAGAAA+AGG - chr4_1:61963113-61963132 None:intergenic 30.0%
! CCTTTTCTCTAATTGGATTA+TGG + chr4_1:61963110-61963129 Msa0562280:intron 30.0%
! ATTCTGCTGTAAAATTTTGC+CGG + chr4_1:61963332-61963351 Msa0562280:intron 30.0%
TATATTAAGTCAACAAGGGT+CGG + chr4_1:61963517-61963536 Msa0562280:intron 30.0%
ATAGAAAATAAATCCGTGCA+TGG + chr4_1:61963868-61963887 Msa0562280:intron 30.0%
! AGCTCAAAGGAAAAGTTTTA+TGG + chr4_1:61964000-61964019 Msa0562280:intron 30.0%
TTAAAACATACGGTGAAAGA+AGG - chr4_1:61964051-61964070 None:intergenic 30.0%
! CTACTATTTTCATAAGAACC+TGG + chr4_1:61964072-61964091 Msa0562280:intron 30.0%
! TATTTTCATAAGAACCTGGT+GGG + chr4_1:61964076-61964095 Msa0562280:intron 30.0%
ATCTATCAAATGGGGAATTT+GGG - chr4_1:61964176-61964195 None:intergenic 30.0%
ATTAAGCCATCTATCAAATG+GGG - chr4_1:61964184-61964203 None:intergenic 30.0%
CAATTAAGCCATCTATCAAA+TGG - chr4_1:61964186-61964205 None:intergenic 30.0%
GAATCATTTCCCTATGTCAT+AGG + chr4_1:61961770-61961789 Msa0562280:intron 35.0%
TGTGCTTTACCTATGACATA+GGG - chr4_1:61961782-61961801 None:intergenic 35.0%
ATGTGCTTTACCTATGACAT+AGG - chr4_1:61961783-61961802 None:intergenic 35.0%
!!! CTTTTGAAGTGAACAGCAAT+TGG + chr4_1:61961953-61961972 Msa0562280:intron 35.0%
! ACTCTAGAAATGAACCATTG+TGG - chr4_1:61962074-61962093 None:intergenic 35.0%
! AAACATACTGCCAAAAGTGT+AGG - chr4_1:61962176-61962195 None:intergenic 35.0%
!! TTCTCTTAAAGTTTTGTCCG+TGG + chr4_1:61962291-61962310 Msa0562280:intron 35.0%
!! ACTAGTTGGTCTTTCTTCTT+TGG - chr4_1:61962390-61962409 None:intergenic 35.0%
!!! AGAAAGACCAACTAGTTTTC+TGG + chr4_1:61962394-61962413 Msa0562280:intron 35.0%
AATCAGACCAGAAAACTAGT+TGG - chr4_1:61962404-61962423 None:intergenic 35.0%
AAGAGTAGGAAGGAAAGAAT+TGG + chr4_1:61962594-61962613 Msa0562280:intron 35.0%
AAGAATTGGAATACATGCCA+TGG + chr4_1:61962608-61962627 Msa0562280:intron 35.0%
ACCAAACATAGGCTATGAAA+GGG - chr4_1:61962747-61962766 None:intergenic 35.0%
AACCAAACATAGGCTATGAA+AGG - chr4_1:61962748-61962767 None:intergenic 35.0%
CCAAACATAGAACCAAACAT+AGG - chr4_1:61962758-61962777 None:intergenic 35.0%
!! CCTATGTTTGGTTCTATGTT+TGG + chr4_1:61962755-61962774 Msa0562280:intron 35.0%
TCAGTGTTCCATTACTTGTA+TGG + chr4_1:61963082-61963101 Msa0562280:intron 35.0%
! CGGAGATCCTAATATTTTAC+CGG - chr4_1:61963354-61963373 None:intergenic 35.0%
GGTGGAAACAAACTTATTCA+TGG - chr4_1:61963780-61963799 None:intergenic 35.0%
!! AAATTGATTTGATTCAGCGG+TGG - chr4_1:61963798-61963817 None:intergenic 35.0%
TGAAGCATAATGAAGCTCAA+AGG + chr4_1:61963987-61964006 Msa0562280:intron 35.0%
! CTATTTTCATAAGAACCTGG+TGG + chr4_1:61964075-61964094 Msa0562280:intron 35.0%
CGTTTATCATTTCTGCAACA+AGG + chr4_1:61964106-61964125 Msa0562280:CDS 35.0%
TCTATCAAATGGGGAATTTG+GGG - chr4_1:61964175-61964194 None:intergenic 35.0%
CATCTATCAAATGGGGAATT+TGG - chr4_1:61964177-61964196 None:intergenic 35.0%
CAAATTCCCCATTTGATAGA+TGG + chr4_1:61964175-61964194 Msa0562280:CDS 35.0%
AGCGCCTCTGTTTGAATATT+TGG + chr4_1:61961748-61961767 Msa0562280:intron 40.0%
! AGCAATTGGTGTGCATCTTT+TGG + chr4_1:61961967-61961986 Msa0562280:intron 40.0%
CTAGAAATGAACCATTGTGG+TGG - chr4_1:61962071-61962090 None:intergenic 40.0%
ATCGTGCATCAAAAAGTCCA+CGG - chr4_1:61962311-61962330 None:intergenic 40.0%
TATCAGAGACCCAAGACTAA+CGG - chr4_1:61962499-61962518 None:intergenic 40.0%
CAAGGGAAGAAATGAAGAGT+AGG + chr4_1:61962580-61962599 Msa0562280:intron 40.0%
GGAAGAAATGAAGAGTAGGA+AGG + chr4_1:61962584-61962603 Msa0562280:intron 40.0%
!!! TTTCTTTTTTCGGCATGCCA+TGG - chr4_1:61962628-61962647 None:intergenic 40.0%
GAGCAAACTATTCAGCACAA+GGG - chr4_1:61962662-61962681 None:intergenic 40.0%
TGAGCAAACTATTCAGCACA+AGG - chr4_1:61962663-61962682 None:intergenic 40.0%
CAAACATAGGCTATGAAAGG+GGG - chr4_1:61962745-61962764 None:intergenic 40.0%
CCAAACATAGGCTATGAAAG+GGG - chr4_1:61962746-61962765 None:intergenic 40.0%
TAGATGGAGGGAATTCTTCA+AGG + chr4_1:61963174-61963193 Msa0562280:intron 40.0%
TCTCACTTTACCTGTGCAAA+CGG - chr4_1:61963549-61963568 None:intergenic 40.0%
GAAAATAAATCCGTGCATGG+TGG + chr4_1:61963871-61963890 Msa0562280:intron 40.0%
ATTTAGTCATCCACCATGCA+CGG - chr4_1:61963884-61963903 None:intergenic 40.0%
!! ATAAACGTTGATTTCCCACC+AGG - chr4_1:61964093-61964112 None:intergenic 40.0%
!! AAGTGCAAGTTGGTAAGAGT+TGG - chr4_1:61964140-61964159 None:intergenic 40.0%
CTATCAAATGGGGAATTTGG+GGG - chr4_1:61964174-61964193 None:intergenic 40.0%
TCACTTTCTCTCCACCACAA+TGG + chr4_1:61962057-61962076 Msa0562280:intron 45.0%
! GTAAACGGGACCTACACTTT+TGG + chr4_1:61962163-61962182 Msa0562280:intron 45.0%
! TGCTGTCAAACCGTTAGTCT+TGG + chr4_1:61962486-61962505 Msa0562280:intron 45.0%
!! GCTGTCAAACCGTTAGTCTT+GGG + chr4_1:61962487-61962506 Msa0562280:intron 45.0%
CTATGAAAGGGGGTCATGAA+AGG - chr4_1:61962735-61962754 None:intergenic 45.0%
CCCCTTTCATAGCCTATGTT+TGG + chr4_1:61962743-61962762 Msa0562280:intron 45.0%
TACACCTCGAGAATCAATGC+TGG - chr4_1:61962786-61962805 None:intergenic 45.0%
AGGGTGAGCCATACAAGTAA+TGG - chr4_1:61963093-61963112 None:intergenic 45.0%
! GGCTCACCCTTTTCTCTAAT+TGG + chr4_1:61963103-61963122 Msa0562280:intron 45.0%
TACGCGTTGTTGCATGTAGA+TGG + chr4_1:61963158-61963177 Msa0562280:intron 45.0%
CGTTGTTGCATGTAGATGGA+GGG + chr4_1:61963162-61963181 Msa0562280:intron 45.0%
GAGACAAATGCTCAAAGCTC+AGG + chr4_1:61963227-61963246 Msa0562280:CDS 45.0%
ACTTTACCTGTGCAAACGGA+AGG - chr4_1:61963545-61963564 None:intergenic 45.0%
!!! ATTTTAATTTCAAAAAATAA+AGG + chr4_1:61963610-61963629 Msa0562280:intron 5.0%
TGAAAGGGGGTCATGAAAGG+AGG - chr4_1:61962732-61962751 None:intergenic 50.0%
GCGTTGTTGCATGTAGATGG+AGG + chr4_1:61963161-61963180 Msa0562280:intron 50.0%
!! GCAGCCAGCATTGATTCTCG+AGG + chr4_1:61962779-61962798 Msa0562280:intron 55.0%
TCGGTACCTTCCGTTTGCAC+AGG + chr4_1:61963536-61963555 Msa0562280:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 61961675 61964267 61961675 ID=Msa0562280;Name=Msa0562280
chr4_1 mRNA 61961675 61964267 61961675 ID=Msa0562280-mRNA-1;Parent=Msa0562280;Name=Msa0562280-mRNA-1;_AED=0.79;_eAED=0.79;_QI=0|0|0|0.5|1|1|4|0|96
chr4_1 exon 61961675 61961682 61961675 ID=Msa0562280-mRNA-1:exon:19583;Parent=Msa0562280-mRNA-1
chr4_1 exon 61963145 61963151 61963145 ID=Msa0562280-mRNA-1:exon:19584;Parent=Msa0562280-mRNA-1
chr4_1 exon 61963177 61963272 61963177 ID=Msa0562280-mRNA-1:exon:19585;Parent=Msa0562280-mRNA-1
chr4_1 exon 61964088 61964267 61964088 ID=Msa0562280-mRNA-1:exon:19586;Parent=Msa0562280-mRNA-1
chr4_1 CDS 61961675 61961682 61961675 ID=Msa0562280-mRNA-1:cds;Parent=Msa0562280-mRNA-1
chr4_1 CDS 61963145 61963151 61963145 ID=Msa0562280-mRNA-1:cds;Parent=Msa0562280-mRNA-1
chr4_1 CDS 61963177 61963272 61963177 ID=Msa0562280-mRNA-1:cds;Parent=Msa0562280-mRNA-1
chr4_1 CDS 61964088 61964267 61964088 ID=Msa0562280-mRNA-1:cds;Parent=Msa0562280-mRNA-1
Gene Sequence

>Msa0562280

ATGTTTGGCATTGATATGGAGGGAATTCTTCAAGGATTTTCTGCATATTATACTAAATTCAATCAGAGACAAATGCTCAAAGCTCAGGAATCAAATGATACTTTTACTTATAACCTGGTGGGAAATCAACGTTTATCATTTCTGCAACAAGGACAATCTACCAACTCTTACCAACTTGCACTTAAATTCAAATTCCCCCAAATTCCCCATTTGATAGATGGCTTAATTGAATTCCCAAAGAAAAAAATCCATTTTGCATTAATACAAAGTAACTTACCAGATAAAAAATAG

Protein sequence

>Msa0562280

MFGIDMEGILQGFSAYYTKFNQRQMLKAQESNDTFTYNLVGNQRLSFLQQGQSTNSYQLALKFKFPQIPHLIDGLIEFPKKKIHFALIQSNLPDKK*