AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268210 MtrunA17_Chr8g0355631 100.000 37 0 0 1 37 35 71 2.22e-20 74.7
Msa0268210 MtrunA17_Chr4g0015761 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa0268210 MtrunA17_CPg0492811 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa0268210 MtrunA17_Chr4g0016861 100.000 35 0 0 1 35 1 35 9.96e-19 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268210 ATCG00600.1 97.222 36 1 0 1 36 1 36 2.23e-18 69.3

Find 14 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATTGAAGTTTCTCTATT+TGG 0.115333 2_2:+70128505 Msa0268210:CDS
TCATGTCAATTGCCAATTCT+TGG 0.245809 2_2:+70128401 Msa0268210:five_prime_UTR
AATAATCCAGCCAAAGTAAT+AGG 0.315680 2_2:-70128547 None:intergenic
AAACTTCAATCATTTGAGTT+TGG 0.328054 2_2:-70128495 None:intergenic
CTAATTCCTATTACTTTGGC+TGG 0.352028 2_2:+70128541 Msa0268210:CDS
TCTCTATTTGGAATCGTGTT+AGG 0.358426 2_2:+70128517 Msa0268210:CDS
AGGTCTAATTCCTATTACTT+TGG 0.388074 2_2:+70128537 Msa0268210:CDS
ATCATTTGAGTTTGGTTGAA+AGG 0.406278 2_2:-70128487 None:intergenic
GAATCTCAATGACCAAGAAT+TGG 0.414372 2_2:-70128413 None:intergenic
AATTGGCAATTGACATGATA+AGG 0.448909 2_2:-70128396 None:intergenic
TTCTTGGTCATTGAGATTCA+CGG 0.471290 2_2:+70128417 Msa0268210:five_prime_UTR
ATATCGACGTGGTGATCAGT+TGG 0.572503 2_2:+70128588 Msa0268210:CDS
TTTGGCTGGATTATTTGTAA+CGG 0.592623 2_2:+70128555 Msa0268210:CDS
GCATATTTACAATATCGACG+TGG 0.789819 2_2:+70128577 Msa0268210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTCAATCATTTGAGTT+TGG - chr2_2:70128498-70128517 None:intergenic 25.0%
! ATGATTGAAGTTTCTCTATT+TGG + chr2_2:70128505-70128524 Msa0268210:CDS 25.0%
AATTGGCAATTGACATGATA+AGG - chr2_2:70128399-70128418 None:intergenic 30.0%
TCAATACAGATTCCTATTGA+AGG + chr2_2:70128440-70128459 Msa0268210:intron 30.0%
GGTAATATCTATCCTTCAAT+AGG - chr2_2:70128455-70128474 None:intergenic 30.0%
TGGTTGAAAGGAAAAAAAGT+GGG - chr2_2:70128478-70128497 None:intergenic 30.0%
TTGGTTGAAAGGAAAAAAAG+TGG - chr2_2:70128479-70128498 None:intergenic 30.0%
! ATCATTTGAGTTTGGTTGAA+AGG - chr2_2:70128490-70128509 None:intergenic 30.0%
AGGTCTAATTCCTATTACTT+TGG + chr2_2:70128537-70128556 Msa0268210:CDS 30.0%
AATAATCCAGCCAAAGTAAT+AGG - chr2_2:70128550-70128569 None:intergenic 30.0%
TTTGGCTGGATTATTTGTAA+CGG + chr2_2:70128555-70128574 Msa0268210:CDS 30.0%
TCATGTCAATTGCCAATTCT+TGG + chr2_2:70128401-70128420 Msa0268210:five_prime_UTR 35.0%
GAATCTCAATGACCAAGAAT+TGG - chr2_2:70128416-70128435 None:intergenic 35.0%
TTCTTGGTCATTGAGATTCA+CGG + chr2_2:70128417-70128436 Msa0268210:five_prime_UTR 35.0%
GTTGAAAGGAAAAAAAGTGG+GGG - chr2_2:70128476-70128495 None:intergenic 35.0%
GGTTGAAAGGAAAAAAAGTG+GGG - chr2_2:70128477-70128496 None:intergenic 35.0%
! TCTCTATTTGGAATCGTGTT+AGG + chr2_2:70128517-70128536 Msa0268210:CDS 35.0%
CTAATTCCTATTACTTTGGC+TGG + chr2_2:70128541-70128560 Msa0268210:CDS 35.0%
GCATATTTACAATATCGACG+TGG + chr2_2:70128577-70128596 Msa0268210:CDS 35.0%
ATATCGACGTGGTGATCAGT+TGG + chr2_2:70128588-70128607 Msa0268210:CDS 45.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70128394 70128615 70128394 ID=Msa0268210;Name=Msa0268210
chr2_2 mRNA 70128394 70128615 70128394 ID=Msa0268210-mRNA-1;Parent=Msa0268210;Name=Msa0268210-mRNA-1;_AED=0.42;_eAED=0.67;_QI=83|0|0|1|0|0|2|0|37
chr2_2 exon 70128394 70128438 70128394 ID=Msa0268210-mRNA-1:exon:22521;Parent=Msa0268210-mRNA-1
chr2_2 exon 70128467 70128615 70128467 ID=Msa0268210-mRNA-1:exon:22522;Parent=Msa0268210-mRNA-1
chr2_2 five_prime_UTR 70128394 70128438 70128394 ID=Msa0268210-mRNA-1:five_prime_utr;Parent=Msa0268210-mRNA-1
chr2_2 five_prime_UTR 70128467 70128504 70128467 ID=Msa0268210-mRNA-1:five_prime_utr;Parent=Msa0268210-mRNA-1
chr2_2 CDS 70128505 70128615 70128505 ID=Msa0268210-mRNA-1:cds;Parent=Msa0268210-mRNA-1
Gene Sequence

>Msa0268210

ATGATTGAAGTTTCTCTATTTGGAATCGTGTTAGGTCTAATTCCTATTACTTTGGCTGGATTATTTGTAACGGCATATTTACAATATCGACGTGGTGATCAGTTGGATATT

Protein sequence

>Msa0268210

MIEVSLFGIVLGLIPITLAGLFVTAYLQYRRGDQLDI