AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686000 MtrunA17_Chr4g0034601 94.574 258 8 1 1 258 1 252 1.12e-180 496
Msa0686000 MtrunA17_Chr5g0403681 64.981 257 83 2 1 256 1 251 5.71e-120 342
Msa0686000 MtrunA17_Chr2g0318961 68.182 198 62 1 57 254 3 199 9.46e-94 274
Msa0686000 MtrunA17_Chr8g0361451 67.172 198 64 1 57 254 3 199 1.66e-92 271
Msa0686000 MtrunA17_Chr5g0429671 63.959 197 71 0 58 254 5 201 2.01e-91 268
Msa0686000 MtrunA17_Chr4g0048841 64.615 195 68 1 60 254 41 234 2.53e-86 256
Msa0686000 MtrunA17_Chr2g0280641 60.914 197 76 1 58 254 4 199 5.48e-81 242
Msa0686000 MtrunA17_Chr8g0361461 68.696 115 36 0 140 254 2 116 2.69e-51 163
Msa0686000 MtrunA17_Chr4g0048831 65.217 115 36 1 140 254 2 112 4.17e-49 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686000 AT4G36750.1 64.706 272 80 1 1 256 1 272 4.46e-121 346
Msa0686000 AT4G27270.1 67.513 197 63 1 58 254 4 199 1.30e-94 277
Msa0686000 AT5G54500.1 67.513 197 63 1 58 254 4 199 1.28e-92 271
Msa0686000 AT4G27270.3 66.667 189 62 1 66 254 1 188 7.07e-89 262
Msa0686000 AT4G27270.2 66.667 189 62 1 66 254 1 188 7.07e-89 262
Msa0686000 AT5G58800.1 56.716 201 87 0 58 258 5 205 5.16e-83 247
Msa0686000 AT5G58800.2 56.716 201 87 0 58 258 5 205 5.16e-83 247
Msa0686000 AT4G27270.4 69.128 149 45 1 106 254 1 148 2.16e-71 216
Msa0686000 AT5G54500.2 66.667 159 52 1 58 216 4 161 3.66e-71 219

Find 78 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGTCATTCATTCTCTTT+TGG 0.135609 4_4:-50317560 Msa0686000:three_prime_UTR
TCAATTGGTCAACTATATTT+TGG 0.158333 4_4:+50319759 None:intergenic
TGGATTTATGAAGGTTAATT+TGG 0.174148 4_4:+50319920 None:intergenic
TTTGATATCTGCTGAGAATT+TGG 0.216664 4_4:-50319374 Msa0686000:CDS
TCCGACGAGGTTTGGTAGTA+TGG 0.226763 4_4:-50319323 Msa0686000:CDS
GTTCCTATTGGATATACTTT+TGG 0.228007 4_4:-50317854 Msa0686000:CDS
CCTCTTACGCTGTAGTTTCT+TGG 0.238361 4_4:+50319191 None:intergenic
GAGGCTGATGGGCTGTTGTT+TGG 0.247789 4_4:-50319349 Msa0686000:CDS
TGTATTTCATGGGAGTTTAT+TGG 0.263678 4_4:-50317462 Msa0686000:three_prime_UTR
AAGCTTGCTGGTGTTCCTGC+TGG 0.298841 4_4:-50319247 Msa0686000:CDS
AGGCTGATGGGCTGTTGTTT+GGG 0.302251 4_4:-50319348 Msa0686000:CDS
GGTTCATTCTCTTCTGTTGT+TGG 0.349593 4_4:+50319679 None:intergenic
TATGACTCAGGTGTATTTCA+TGG 0.357466 4_4:-50317473 Msa0686000:three_prime_UTR
AATCTTGTTGTGTTTATTGT+TGG 0.373601 4_4:-50317609 Msa0686000:three_prime_UTR
AAAGGGGTTGATTCTGTTGA+TGG 0.375228 4_4:-50319487 Msa0686000:CDS
TTTGAAATGGAATCAATTAG+AGG 0.381780 4_4:-50317821 Msa0686000:CDS
TTGATATTTCAAAATTTCAA+TGG 0.384573 4_4:-50319740 Msa0686000:exon
GGAATGCTCTATGTTCCTAT+TGG 0.388744 4_4:-50317866 Msa0686000:CDS
TGATATTTCAAAATTTCAAT+GGG 0.396895 4_4:-50319739 Msa0686000:exon
AGCTTGCTGGTGTTCCTGCT+GGG 0.399729 4_4:-50319246 Msa0686000:CDS
GAGAATTTGGTGGAGGCTGA+TGG 0.410033 4_4:-50319361 Msa0686000:CDS
AACAAATGTTGGATTTATGA+AGG 0.414289 4_4:+50319911 None:intergenic
ATTAGTTCCAACACTTCCTT+TGG 0.423053 4_4:+50319427 None:intergenic
TTTGGGTTTCCGACGAGGTT+TGG 0.429899 4_4:-50319331 Msa0686000:CDS
GCTAATATAGTCAAGAAGTT+AGG 0.438947 4_4:-50317701 Msa0686000:CDS
GCACCAAAAGTATATCCAAT+AGG 0.439235 4_4:+50317851 None:intergenic
TGGAAGGAACAGAAGCTTGC+TGG 0.451653 4_4:-50319259 Msa0686000:CDS
GATTCTGTTGATGGAGTTGA+AGG 0.452417 4_4:-50319478 Msa0686000:CDS
CAATGCAACGTGAAGGTGCT+TGG 0.454677 4_4:+50320025 None:intergenic
TGGTGCTGGAATGTTTGAAA+TGG 0.456592 4_4:-50317834 Msa0686000:CDS
GCATAGGTTCCAGCACCATA+TGG 0.464417 4_4:+50317791 None:intergenic
CTTGTTGTGTTTATTGTTGG+AGG 0.474131 4_4:-50317606 Msa0686000:three_prime_UTR
TAGTATGGTGGCGCAGATGA+AGG 0.477857 4_4:-50319308 Msa0686000:CDS
GATATCTGCTGAGAATTTGG+TGG 0.481344 4_4:-50319371 Msa0686000:CDS
ATTAGAGGTGGAACTCCATA+TGG 0.481721 4_4:-50317806 Msa0686000:CDS
GGAACCTCATCATCTTTCTC+AGG 0.484933 4_4:+50319397 None:intergenic
AGAATTTGGTGGAGGCTGAT+GGG 0.488576 4_4:-50319360 Msa0686000:CDS
TTTGTTAGTACTGGTACTCA+AGG 0.489722 4_4:-50319220 Msa0686000:CDS
TTATAATAAATTTATGACTC+AGG 0.491672 4_4:-50317485 Msa0686000:three_prime_UTR
ATTTCAATGGGGAAAGGAAG+AGG 0.491973 4_4:-50319727 Msa0686000:exon
ATGACTCAGGTGTATTTCAT+GGG 0.501897 4_4:-50317472 Msa0686000:three_prime_UTR
TTCAAAATTTCAATGGGGAA+AGG 0.501971 4_4:-50319733 Msa0686000:exon
TATGGTGCTGGAACCTATGC+AGG 0.505168 4_4:-50317788 Msa0686000:CDS
TCTTGAACCATCTCCTGCAT+AGG 0.505315 4_4:+50317775 None:intergenic
GTATGGTCATGTGGAATCAT+TGG 0.514794 4_4:-50319524 Msa0686000:CDS
GTTGGAGGTATGAGATTGGT+AGG 0.521048 4_4:-50317591 Msa0686000:three_prime_UTR
ACAAGCAACTGAAGCTGAGT+TGG 0.528852 4_4:-50317753 Msa0686000:CDS
TATTGTTGGAGGTATGAGAT+TGG 0.530884 4_4:-50317595 Msa0686000:three_prime_UTR
TCGATTCCACAGGGCAGTTG+TGG 0.537460 4_4:-50319279 Msa0686000:CDS
GACGCTGCCAAAGGAAGTGT+TGG 0.543102 4_4:-50319434 Msa0686000:CDS
AGTATGGTGGCGCAGATGAA+GGG 0.543981 4_4:-50319307 Msa0686000:CDS
GGTGGAACTCCATATGGTGC+TGG 0.548136 4_4:-50317800 Msa0686000:CDS
TTATTCAATGTATGGTCATG+TGG 0.560406 4_4:-50319533 Msa0686000:CDS
TTGGCACTTGCTGAATATCA+AGG 0.565677 4_4:-50317734 Msa0686000:CDS
ATCTGCTGAGAATTTGGTGG+AGG 0.570664 4_4:-50319368 Msa0686000:CDS
AAGACAGAGAGGTGGGTGGG+TGG 0.571460 4_4:+50319998 None:intergenic
TTCCACAGGGCAGTTGTGGA+AGG 0.579570 4_4:-50319275 Msa0686000:CDS
GAAATGGAATCAATTAGAGG+TGG 0.580051 4_4:-50317818 Msa0686000:CDS
GTTAGTACTGGTACTCAAGG+TGG 0.582457 4_4:-50319217 Msa0686000:CDS
ATTCTGTTGATGGAGTTGAA+GGG 0.590324 4_4:-50319477 Msa0686000:CDS
TTCCTTCCACAACTGCCCTG+TGG 0.593676 4_4:+50319273 None:intergenic
TGTTGTTTGGGTTTCCGACG+AGG 0.600440 4_4:-50319336 Msa0686000:CDS
AGTACTGGTACTCAAGGTGG+TGG 0.615053 4_4:-50319214 Msa0686000:CDS
TGTCGTAGAGACGCTGCCAA+AGG 0.620906 4_4:-50319443 Msa0686000:CDS
GACGAGGTTTGGTAGTATGG+TGG 0.625698 4_4:-50319320 Msa0686000:CDS
AGATGTAGCAACAATAGATG+TGG 0.630772 4_4:-50319605 Msa0686000:CDS
AGCACCTGAGAAAGATGATG+AGG 0.634435 4_4:-50319401 Msa0686000:CDS
GCTGGAACCTATGCAGGAGA+TGG 0.635685 4_4:-50317782 Msa0686000:CDS
CCAAAAGAGAATGAATGACA+AGG 0.639478 4_4:+50317560 None:intergenic
TGAATATCAAGGTAGATACA+TGG 0.639975 4_4:-50317723 Msa0686000:CDS
AAATTCTCAGCAGATATCAA+AGG 0.642913 4_4:+50319376 None:intergenic
TAGGAACATAGAGCATTCCA+TGG 0.644524 4_4:+50317870 None:intergenic
ACCATACTACCAAACCTCGT+CGG 0.654426 4_4:+50319322 None:intergenic
GATATTTCAAAATTTCAATG+GGG 0.665834 4_4:-50319738 Msa0686000:exon
ATAACACAATTAGTCCACCA+TGG 0.694726 4_4:-50317887 Msa0686000:CDS
TGGAAAACAATGCAACGTGA+AGG 0.695980 4_4:+50320018 None:intergenic
GAACATAGAGCATTCCATGG+TGG 0.721594 4_4:+50317873 None:intergenic
TTGGCAGCGTCTCTACGACA+CGG 0.741183 4_4:+50319446 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAATTATAAAAACTTT+AGG + chr4_4:50318377-50318396 None:intergenic 10.0%
!!! TGTGAATAAATTTTATGTAA+TGG + chr4_4:50317577-50317596 None:intergenic 15.0%
!!! TGAAATTTTGAAATATCAAT+TGG + chr4_4:50317780-50317799 None:intergenic 15.0%
!! TTGATATTTCAAAATTTCAA+TGG - chr4_4:50317781-50317800 Msa0686000:CDS 15.0%
!! TGATATTTCAAAATTTCAAT+GGG - chr4_4:50317782-50317801 Msa0686000:CDS 15.0%
!!! ATTGTTTTTTATTCAATGTA+TGG - chr4_4:50317980-50317999 Msa0686000:intron 15.0%
!! AGGAAAACAAAAAAAATTAA+AGG + chr4_4:50318357-50318376 None:intergenic 15.0%
!!! TATTTTTTTTAGTATGAGTA+TGG - chr4_4:50318887-50318906 Msa0686000:intron 15.0%
!!! ATTTTTTTTAGTATGAGTAT+GGG - chr4_4:50318888-50318907 Msa0686000:intron 15.0%
!! TTATAATAAATTTATGACTC+AGG - chr4_4:50320036-50320055 Msa0686000:five_prime_UTR 15.0%
!!! TTTTCCTTTTCTTTTTCTTT+TGG - chr4_4:50317545-50317564 Msa0686000:three_prime_UTR 20.0%
!! GTGTATTAAAAAACAAATGT+TGG + chr4_4:50317624-50317643 None:intergenic 20.0%
!! GATATTTCAAAATTTCAATG+GGG - chr4_4:50317783-50317802 Msa0686000:CDS 20.0%
!!! ATATTTTTGTTGTTAGTTGA+AGG + chr4_4:50318637-50318656 None:intergenic 20.0%
!!! TTGTTATTAGTTAAATGTTG+TGG - chr4_4:50318656-50318675 Msa0686000:intron 20.0%
! ATGACCAAAAGAAAAAGAAA+AGG + chr4_4:50317552-50317571 None:intergenic 25.0%
! TGGATTTATGAAGGTTAATT+TGG + chr4_4:50317604-50317623 None:intergenic 25.0%
! AACAAATGTTGGATTTATGA+AGG + chr4_4:50317613-50317632 None:intergenic 25.0%
!! TCAATTGGTCAACTATATTT+TGG + chr4_4:50317765-50317784 None:intergenic 25.0%
!! TTGTTTCATTTTGTGGAATT+TGG + chr4_4:50317883-50317902 None:intergenic 25.0%
! ATATAGAAGTTGGAGTAATA+AGG - chr4_4:50318594-50318613 Msa0686000:intron 25.0%
!! AGTATGGGTAATAGTTATTT+TGG - chr4_4:50318903-50318922 Msa0686000:intron 25.0%
! TCTAGACATTTGATATATGT+GGG + chr4_4:50319166-50319185 None:intergenic 25.0%
! ATCTAGACATTTGATATATG+TGG + chr4_4:50319167-50319186 None:intergenic 25.0%
! AACTCAAATAGTCAAATTTG+AGG - chr4_4:50319201-50319220 Msa0686000:CDS 25.0%
!!! AAAGTGTTTTTAGACTACTT+TGG - chr4_4:50319281-50319300 Msa0686000:CDS 25.0%
! AATTTCTATTGTTATGTAGC+TGG - chr4_4:50319605-50319624 Msa0686000:CDS 25.0%
!! TTTGAAATGGAATCAATTAG+AGG - chr4_4:50319700-50319719 Msa0686000:CDS 25.0%
!! AATCTTGTTGTGTTTATTGT+TGG - chr4_4:50319912-50319931 Msa0686000:five_prime_UTR 25.0%
TTCAAAATTTCAATGGGGAA+AGG - chr4_4:50317788-50317807 Msa0686000:CDS 30.0%
!!! ATTTGGTTTTCAACACTAAG+AGG + chr4_4:50317866-50317885 None:intergenic 30.0%
!!! CTACTTGTTGTTTCATTTTG+TGG + chr4_4:50317890-50317909 None:intergenic 30.0%
TTATTCAATGTATGGTCATG+TGG - chr4_4:50317988-50318007 Msa0686000:intron 30.0%
TTGGCAAGATCATTGAAAAA+AGG - chr4_4:50318016-50318035 Msa0686000:intron 30.0%
TGGCAAGATCATTGAAAAAA+GGG - chr4_4:50318017-50318036 Msa0686000:intron 30.0%
AAATTCTCAGCAGATATCAA+AGG + chr4_4:50318148-50318167 None:intergenic 30.0%
TTTGATATCTGCTGAGAATT+TGG - chr4_4:50318147-50318166 Msa0686000:intron 30.0%
TTACGAGTTATAAGGTGATT+TGG - chr4_4:50318555-50318574 Msa0686000:intron 30.0%
TAACATTAATCCTAAAGTCG+AGG + chr4_4:50318786-50318805 None:intergenic 30.0%
!! CGACTTTAGGATTAATGTTA+TGG - chr4_4:50318786-50318805 Msa0686000:intron 30.0%
ATCTATTCAAAGGTTATGTG+TGG - chr4_4:50318840-50318859 Msa0686000:intron 30.0%
GACTAATAAAGTGCAAAATC+AGG + chr4_4:50318991-50319010 None:intergenic 30.0%
CTAGACATTTGATATATGTG+GGG + chr4_4:50319165-50319184 None:intergenic 30.0%
!! CTTCTACTCTAAAATTACCT+AGG + chr4_4:50319231-50319250 None:intergenic 30.0%
AATGGAGGAACTAATTAGTT+GGG - chr4_4:50319401-50319420 Msa0686000:CDS 30.0%
ACAAGCCAATGAATCAATAT+GGG - chr4_4:50319499-50319518 Msa0686000:CDS 30.0%
! GTTCCTATTGGATATACTTT+TGG - chr4_4:50319667-50319686 Msa0686000:CDS 30.0%
TGAATATCAAGGTAGATACA+TGG - chr4_4:50319798-50319817 Msa0686000:five_prime_UTR 30.0%
GCTAATATAGTCAAGAAGTT+AGG - chr4_4:50319820-50319839 Msa0686000:five_prime_UTR 30.0%
TGTATTTCATGGGAGTTTAT+TGG - chr4_4:50320059-50320078 Msa0686000:five_prime_UTR 30.0%
AGAAAAGGAAAAAGACAGAG+AGG + chr4_4:50317537-50317556 None:intergenic 35.0%
AGATGTAGCAACAATAGATG+TGG - chr4_4:50317916-50317935 Msa0686000:intron 35.0%
GGCAAGATCATTGAAAAAAG+GGG - chr4_4:50318018-50318037 Msa0686000:intron 35.0%
! ATTCTGTTGATGGAGTTGAA+GGG - chr4_4:50318044-50318063 Msa0686000:intron 35.0%
ATTAGTTCCAACACTTCCTT+TGG + chr4_4:50318097-50318116 None:intergenic 35.0%
!! TTTGTTAGTACTGGTACTCA+AGG - chr4_4:50318301-50318320 Msa0686000:intron 35.0%
CACTGCTATTACGAGTTATA+AGG - chr4_4:50318547-50318566 Msa0686000:intron 35.0%
!! TATAAGGTGATTTGGTGGTT+TGG - chr4_4:50318563-50318582 Msa0686000:intron 35.0%
!!! TCTCATGAGTTTGTTTTGTG+GGG + chr4_4:50318733-50318752 None:intergenic 35.0%
!!! CTCTCATGAGTTTGTTTTGT+GGG + chr4_4:50318734-50318753 None:intergenic 35.0%
TGTGATTAAACCTCGACTTT+AGG - chr4_4:50318773-50318792 Msa0686000:intron 35.0%
ATTAATGTTATGGCGAACCA+GGG - chr4_4:50318796-50318815 Msa0686000:intron 35.0%
GTCAAATTTGAGGAATACCT+AGG - chr4_4:50319211-50319230 Msa0686000:CDS 35.0%
TCATGCCAACCAAATATTTG+TGG - chr4_4:50319377-50319396 Msa0686000:CDS 35.0%
CATTGCCACAAATATTTGGT+TGG + chr4_4:50319385-50319404 None:intergenic 35.0%
CAACCAAATATTTGTGGCAA+TGG - chr4_4:50319383-50319402 Msa0686000:CDS 35.0%
CAATGGAGGAACTAATTAGT+TGG - chr4_4:50319400-50319419 Msa0686000:CDS 35.0%
ATGGAGGAACTAATTAGTTG+GGG - chr4_4:50319402-50319421 Msa0686000:CDS 35.0%
! GGTATGATTGAAGGACTTTT+TGG - chr4_4:50319442-50319461 Msa0686000:CDS 35.0%
! AAGGACTTTTTGGACTATAG+AGG - chr4_4:50319452-50319471 Msa0686000:CDS 35.0%
!! CATATTGATTCATTGGCTTG+TGG + chr4_4:50319500-50319519 None:intergenic 35.0%
CACAAGCCAATGAATCAATA+TGG - chr4_4:50319498-50319517 Msa0686000:CDS 35.0%
CAAGCCAATGAATCAATATG+GGG - chr4_4:50319500-50319519 Msa0686000:CDS 35.0%
ATAACACAATTAGTCCACCA+TGG - chr4_4:50319634-50319653 Msa0686000:CDS 35.0%
! GCACCAAAAGTATATCCAAT+AGG + chr4_4:50319673-50319692 None:intergenic 35.0%
!! ATTGGATATACTTTTGGTGC+TGG - chr4_4:50319673-50319692 Msa0686000:CDS 35.0%
GAAATGGAATCAATTAGAGG+TGG - chr4_4:50319703-50319722 Msa0686000:CDS 35.0%
!!! TCAAGAGAAACTAGCTTTTC+TGG + chr4_4:50319846-50319865 None:intergenic 35.0%
! CTTGTTGTGTTTATTGTTGG+AGG - chr4_4:50319915-50319934 Msa0686000:five_prime_UTR 35.0%
! TATTGTTGGAGGTATGAGAT+TGG - chr4_4:50319926-50319945 Msa0686000:five_prime_UTR 35.0%
CCAAAAGAGAATGAATGACA+AGG + chr4_4:50319964-50319983 None:intergenic 35.0%
! CCTTGTCATTCATTCTCTTT+TGG - chr4_4:50319961-50319980 Msa0686000:five_prime_UTR 35.0%
! TGTCATTCATTCTCTTTTGG+AGG - chr4_4:50319964-50319983 Msa0686000:five_prime_UTR 35.0%
TATGACTCAGGTGTATTTCA+TGG - chr4_4:50320048-50320067 Msa0686000:five_prime_UTR 35.0%
ATGACTCAGGTGTATTTCAT+GGG - chr4_4:50320049-50320068 Msa0686000:five_prime_UTR 35.0%
TGGAAAACAATGCAACGTGA+AGG + chr4_4:50317506-50317525 None:intergenic 40.0%
AAGGAAAAAGACAGAGAGGT+GGG + chr4_4:50317533-50317552 None:intergenic 40.0%
AAAGGAAAAAGACAGAGAGG+TGG + chr4_4:50317534-50317553 None:intergenic 40.0%
ATTTCAATGGGGAAAGGAAG+AGG - chr4_4:50317794-50317813 Msa0686000:CDS 40.0%
!!! GTTGGTGCTTTTTTCTTGCT+TGG + chr4_4:50317827-50317846 None:intergenic 40.0%
GGTTCATTCTCTTCTGTTGT+TGG + chr4_4:50317845-50317864 None:intergenic 40.0%
GTATGGTCATGTGGAATCAT+TGG - chr4_4:50317997-50318016 Msa0686000:intron 40.0%
! AAAGGGGTTGATTCTGTTGA+TGG - chr4_4:50318034-50318053 Msa0686000:intron 40.0%
! GATTCTGTTGATGGAGTTGA+AGG - chr4_4:50318043-50318062 Msa0686000:intron 40.0%
GATATCTGCTGAGAATTTGG+TGG - chr4_4:50318150-50318169 Msa0686000:intron 40.0%
GTACTAACAAAAAACCCAGC+AGG + chr4_4:50318292-50318311 None:intergenic 40.0%
!!! GCTGGGTTTTTTGTTAGTAC+TGG - chr4_4:50318292-50318311 Msa0686000:intron 40.0%
CAAGAAACTACAGCGTAAGA+GGG - chr4_4:50318331-50318350 Msa0686000:intron 40.0%
! CGAGTTATAAGGTGATTTGG+TGG - chr4_4:50318558-50318577 Msa0686000:intron 40.0%
GGAAGGAGGAATATAGAAGT+TGG - chr4_4:50318584-50318603 Msa0686000:intron 40.0%
!!! CCTCTCATGAGTTTGTTTTG+TGG + chr4_4:50318735-50318754 None:intergenic 40.0%
CCACAAAACAAACTCATGAG+AGG - chr4_4:50318732-50318751 Msa0686000:intron 40.0%
GATTAATGTTATGGCGAACC+AGG - chr4_4:50318795-50318814 Msa0686000:intron 40.0%
TTAATGTTATGGCGAACCAG+GGG - chr4_4:50318797-50318816 Msa0686000:intron 40.0%
CCTCCATTGCCACAAATATT+TGG + chr4_4:50319389-50319408 None:intergenic 40.0%
CCAAATATTTGTGGCAATGG+AGG - chr4_4:50319386-50319405 Msa0686000:CDS 40.0%
TGGAGGAACTAATTAGTTGG+GGG - chr4_4:50319403-50319422 Msa0686000:CDS 40.0%
GAACTAATTAGTTGGGGGTA+GGG - chr4_4:50319408-50319427 Msa0686000:CDS 40.0%
AACTAATTAGTTGGGGGTAG+GGG - chr4_4:50319409-50319428 Msa0686000:CDS 40.0%
!! CCTACCCCATATTGATTCAT+TGG + chr4_4:50319507-50319526 None:intergenic 40.0%
CCAATGAATCAATATGGGGT+AGG - chr4_4:50319504-50319523 Msa0686000:CDS 40.0%
! TAGGAACATAGAGCATTCCA+TGG + chr4_4:50319654-50319673 None:intergenic 40.0%
GGAATGCTCTATGTTCCTAT+TGG - chr4_4:50319655-50319674 Msa0686000:CDS 40.0%
! TGGTGCTGGAATGTTTGAAA+TGG - chr4_4:50319687-50319706 Msa0686000:CDS 40.0%
ATTAGAGGTGGAACTCCATA+TGG - chr4_4:50319715-50319734 Msa0686000:CDS 40.0%
! TTGGCACTTGCTGAATATCA+AGG - chr4_4:50319787-50319806 Msa0686000:five_prime_UTR 40.0%
AAAAAGACAGAGAGGTGGGT+GGG + chr4_4:50317529-50317548 None:intergenic 45.0%
AGCACCTGAGAAAGATGATG+AGG - chr4_4:50318120-50318139 Msa0686000:intron 45.0%
GGAACCTCATCATCTTTCTC+AGG + chr4_4:50318127-50318146 None:intergenic 45.0%
! ATCTGCTGAGAATTTGGTGG+AGG - chr4_4:50318153-50318172 Msa0686000:intron 45.0%
! AGAATTTGGTGGAGGCTGAT+GGG - chr4_4:50318161-50318180 Msa0686000:intron 45.0%
ACCATACTACCAAACCTCGT+CGG + chr4_4:50318202-50318221 None:intergenic 45.0%
! AAGGGACTTTTCGATTCCAC+AGG - chr4_4:50318232-50318251 Msa0686000:intron 45.0%
! AGGGACTTTTCGATTCCACA+GGG - chr4_4:50318233-50318252 Msa0686000:intron 45.0%
!! GTTAGTACTGGTACTCAAGG+TGG - chr4_4:50318304-50318323 Msa0686000:intron 45.0%
CCTCTTACGCTGTAGTTTCT+TGG + chr4_4:50318333-50318352 None:intergenic 45.0%
CCAAGAAACTACAGCGTAAG+AGG - chr4_4:50318330-50318349 Msa0686000:intron 45.0%
!! AGGTGATTTGGTGGTTTGGA+AGG - chr4_4:50318567-50318586 Msa0686000:intron 45.0%
!! TGATTTGGTGGTTTGGAAGG+AGG - chr4_4:50318570-50318589 Msa0686000:intron 45.0%
TGTGGCTCAATTGACAAGAC+AGG - chr4_4:50318674-50318693 Msa0686000:intron 45.0%
! ACAAGACAGGTTGAGCTTGT+GGG - chr4_4:50318687-50318706 Msa0686000:intron 45.0%
GAAAGGGCGGATCTATTCAA+AGG - chr4_4:50318830-50318849 Msa0686000:intron 45.0%
AATGTCTAGATCCGTCCATC+TGG - chr4_4:50319176-50319195 Msa0686000:intron 45.0%
! ATTTGAGTTCGCCAGATGGA+CGG + chr4_4:50319190-50319209 None:intergenic 45.0%
! GACTATTTGAGTTCGCCAGA+TGG + chr4_4:50319194-50319213 None:intergenic 45.0%
TACGATACCACATGTGCTTG+TGG - chr4_4:50319348-50319367 Msa0686000:CDS 45.0%
ACACAAACCACAAGCACATG+TGG + chr4_4:50319358-50319377 None:intergenic 45.0%
GGAACTAATTAGTTGGGGGT+AGG - chr4_4:50319407-50319426 Msa0686000:CDS 45.0%
! CAATGGTGGGGTATGATTGA+AGG - chr4_4:50319433-50319452 Msa0686000:CDS 45.0%
! GAACATAGAGCATTCCATGG+TGG + chr4_4:50319651-50319670 None:intergenic 45.0%
TCTTGAACCATCTCCTGCAT+AGG + chr4_4:50319749-50319768 None:intergenic 45.0%
ACAAGCAACTGAAGCTGAGT+TGG - chr4_4:50319768-50319787 Msa0686000:five_prime_UTR 45.0%
! GTTGGAGGTATGAGATTGGT+AGG - chr4_4:50319930-50319949 Msa0686000:five_prime_UTR 45.0%
!! CAATGCAACGTGAAGGTGCT+TGG + chr4_4:50317499-50317518 None:intergenic 50.0%
GAAAAAGACAGAGAGGTGGG+TGG + chr4_4:50317530-50317549 None:intergenic 50.0%
! GAGAATTTGGTGGAGGCTGA+TGG - chr4_4:50318160-50318179 Msa0686000:intron 50.0%
!! AGGCTGATGGGCTGTTGTTT+GGG - chr4_4:50318173-50318192 Msa0686000:intron 50.0%
! TGTTGTTTGGGTTTCCGACG+AGG - chr4_4:50318185-50318204 Msa0686000:intron 50.0%
! TTTGGGTTTCCGACGAGGTT+TGG - chr4_4:50318190-50318209 Msa0686000:intron 50.0%
! TCCGACGAGGTTTGGTAGTA+TGG - chr4_4:50318198-50318217 Msa0686000:intron 50.0%
! GACGAGGTTTGGTAGTATGG+TGG - chr4_4:50318201-50318220 Msa0686000:intron 50.0%
! TAGTATGGTGGCGCAGATGA+AGG - chr4_4:50318213-50318232 Msa0686000:intron 50.0%
! AGTATGGTGGCGCAGATGAA+GGG - chr4_4:50318214-50318233 Msa0686000:intron 50.0%
TGGAAGGAACAGAAGCTTGC+TGG - chr4_4:50318262-50318281 Msa0686000:intron 50.0%
!! AGTACTGGTACTCAAGGTGG+TGG - chr4_4:50318307-50318326 Msa0686000:intron 50.0%
! GACAAGACAGGTTGAGCTTG+TGG - chr4_4:50318686-50318705 Msa0686000:intron 50.0%
CAGGGGCGGATCTATTGAAA+GGG - chr4_4:50318814-50318833 Msa0686000:intron 50.0%
GCATAGGTTCCAGCACCATA+TGG + chr4_4:50319733-50319752 None:intergenic 50.0%
! TATGGTGCTGGAACCTATGC+AGG - chr4_4:50319733-50319752 Msa0686000:exon 50.0%
TTGGCAGCGTCTCTACGACA+CGG + chr4_4:50318078-50318097 None:intergenic 55.0%
TGTCGTAGAGACGCTGCCAA+AGG - chr4_4:50318078-50318097 Msa0686000:intron 55.0%
! GACGCTGCCAAAGGAAGTGT+TGG - chr4_4:50318087-50318106 Msa0686000:intron 55.0%
!! GAGGCTGATGGGCTGTTGTT+TGG - chr4_4:50318172-50318191 Msa0686000:intron 55.0%
TCGATTCCACAGGGCAGTTG+TGG - chr4_4:50318242-50318261 Msa0686000:intron 55.0%
TTCCACAGGGCAGTTGTGGA+AGG - chr4_4:50318246-50318265 Msa0686000:intron 55.0%
TTCCTTCCACAACTGCCCTG+TGG + chr4_4:50318251-50318270 None:intergenic 55.0%
!! AAGCTTGCTGGTGTTCCTGC+TGG - chr4_4:50318274-50318293 Msa0686000:intron 55.0%
!! AGCTTGCTGGTGTTCCTGCT+GGG - chr4_4:50318275-50318294 Msa0686000:intron 55.0%
AGGTTGAGCTTGTGGGTGAG+AGG - chr4_4:50318694-50318713 Msa0686000:intron 55.0%
ATGTTATGGCGAACCAGGGG+CGG - chr4_4:50318800-50318819 Msa0686000:intron 55.0%
CCTTTCAATAGATCCGCCCC+TGG + chr4_4:50318816-50318835 None:intergenic 55.0%
CCAGGGGCGGATCTATTGAA+AGG - chr4_4:50318813-50318832 Msa0686000:intron 55.0%
GGGCGGATCTATTGAAAGGG+CGG - chr4_4:50318817-50318836 Msa0686000:intron 55.0%
TAGTTGGGGGTAGGGGTCAA+TGG - chr4_4:50319416-50319435 Msa0686000:CDS 55.0%
GGTGGAACTCCATATGGTGC+TGG - chr4_4:50319721-50319740 Msa0686000:CDS 55.0%
GCTGGAACCTATGCAGGAGA+TGG - chr4_4:50319739-50319758 Msa0686000:exon 55.0%
AAGACAGAGAGGTGGGTGGG+TGG + chr4_4:50317526-50317545 None:intergenic 60.0%
TTGGGGGTAGGGGTCAATGG+TGG - chr4_4:50319419-50319438 Msa0686000:CDS 60.0%
TGGGGGTAGGGGTCAATGGT+GGG - chr4_4:50319420-50319439 Msa0686000:CDS 60.0%
GGGGGTAGGGGTCAATGGTG+GGG - chr4_4:50319421-50319440 Msa0686000:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 50317454 50320089 50317454 ID=Msa0686000;Name=Msa0686000
chr4_4 mRNA 50317454 50320089 50317454 ID=Msa0686000-mRNA-1;Parent=Msa0686000;Name=Msa0686000-mRNA-1;_AED=0.01;_eAED=0.01;_QI=346|1|1|1|1|1|2|234|258
chr4_4 exon 50317454 50317919 50317454 ID=Msa0686000-mRNA-1:exon:12857;Parent=Msa0686000-mRNA-1
chr4_4 exon 50319199 50320089 50319199 ID=Msa0686000-mRNA-1:exon:12856;Parent=Msa0686000-mRNA-1
chr4_4 five_prime_UTR 50319744 50320089 50319744 ID=Msa0686000-mRNA-1:five_prime_utr;Parent=Msa0686000-mRNA-1
chr4_4 CDS 50319199 50319743 50319199 ID=Msa0686000-mRNA-1:cds;Parent=Msa0686000-mRNA-1
chr4_4 CDS 50317688 50317919 50317688 ID=Msa0686000-mRNA-1:cds;Parent=Msa0686000-mRNA-1
chr4_4 three_prime_UTR 50317454 50317687 50317454 ID=Msa0686000-mRNA-1:three_prime_utr;Parent=Msa0686000-mRNA-1
Gene Sequence

>Msa0686000

ATGGGGAAAGGAAGAGGTTGCATACCAAGCAAGAAAAAAGCACCAACAACAGAAGAGAATGAACCTCTTAGTGTTGAAAACCAAATTCCACAAAATGAAACAACAAGTAGAAGCATAGATGTAGCAACAATAGATGTGGCAACAACATCAGAAGAAGCAGTGAAAAAGCTCAAAGTTTACATTGTTTTTTATTCAATGTATGGTCATGTGGAATCATTGGCAAGATCATTGAAAAAAGGGGTTGATTCTGTTGATGGAGTTGAAGGGTTTTTGTACCGTGTCGTAGAGACGCTGCCAAAGGAAGTGTTGGAACTAATGAAAGCACCTGAGAAAGATGATGAGGTTCCTTTGATATCTGCTGAGAATTTGGTGGAGGCTGATGGGCTGTTGTTTGGGTTTCCGACGAGGTTTGGTAGTATGGTGGCGCAGATGAAGGGACTTTTCGATTCCACAGGGCAGTTGTGGAAGGAACAGAAGCTTGCTGGTGTTCCTGCTGGGTTTTTTGTTAGTACTGGTACTCAAGGTGGTGGCCAAGAAACTACAGCCTGGACAGCAATAACACAATTAGTCCACCATGGAATGCTCTATGTTCCTATTGGATATACTTTTGGTGCTGGAATGTTTGAAATGGAATCAATTAGAGGTGGAACTCCATATGGTGCTGGAACCTATGCAGGAGATGGTTCAAGACAAGCAACTGAAGCTGAGTTGGCACTTGCTGAATATCAAGGTAGATACATGGCTAATATAGTCAAGAAGTTAGGCCAGAAAAGCTAG
Protein sequence

>Msa0686000

MGKGRGCIPSKKKAPTTEENEPLSVENQIPQNETTSRSIDVATIDVATTSEEAVKKLKVYIVFYSMYGHVESLARSLKKGVDSVDGVEGFLYRVVETLPKEVLELMKAPEKDDEVPLISAENLVEADGLLFGFPTRFGSMVAQMKGLFDSTGQLWKEQKLAGVPAGFFVSTGTQGGGQETTAWTAITQLVHHGMLYVPIGYTFGAGMFEMESIRGGTPYGAGTYAGDGSRQATEAELALAEYQGRYMANIVKKLGQKS*