AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605370 MtrunA17_Chr4g0034601 95.041 242 6 1 1 242 1 236 2.98e-169 466
Msa0605370 MtrunA17_Chr5g0403681 66.255 243 75 2 1 242 1 237 5.47e-114 327
Msa0605370 MtrunA17_Chr8g0361451 69.022 184 56 1 57 240 3 185 6.45e-88 259
Msa0605370 MtrunA17_Chr2g0318961 69.565 184 55 1 57 240 3 185 9.45e-88 258
Msa0605370 MtrunA17_Chr5g0429671 64.865 185 65 0 58 242 5 189 3.52e-86 254
Msa0605370 MtrunA17_Chr4g0048841 65.746 181 61 1 60 240 41 220 1.33e-80 241
Msa0605370 MtrunA17_Chr2g0280641 61.202 183 70 1 58 240 4 185 4.87e-75 226
Msa0605370 MtrunA17_Chr8g0361461 72.449 98 27 0 140 237 2 99 3.91e-46 149
Msa0605370 MtrunA17_Chr4g0048831 68.317 101 28 1 140 240 2 98 9.46e-44 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605370 AT4G36750.1 65.504 258 73 2 1 242 1 258 2.17e-114 329
Msa0605370 AT4G27270.1 68.852 183 56 1 58 240 4 185 4.56e-89 262
Msa0605370 AT5G54500.1 68.852 183 56 1 58 240 4 185 7.76e-87 256
Msa0605370 AT4G27270.3 68.000 175 55 1 66 240 1 174 3.96e-83 246
Msa0605370 AT4G27270.2 68.000 175 55 1 66 240 1 174 3.96e-83 246
Msa0605370 AT5G58800.1 58.378 185 77 0 58 242 5 189 9.74e-78 233
Msa0605370 AT5G58800.2 58.378 185 77 0 58 242 5 189 9.74e-78 233
Msa0605370 AT5G54500.2 66.250 160 53 1 58 217 4 162 1.09e-71 219
Msa0605370 AT4G27270.4 71.111 135 38 1 106 240 1 134 1.18e-65 201

Find 81 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTGGTCAACTATATTT+TGG 0.158333 4_2:+51179601 None:intergenic
CCTTGTCATTCATTCTCTTC+TGG 0.158992 4_2:-51177212 Msa0605370:three_prime_UTR
TGGATTTATGAAGGTTAATT+TGG 0.174148 4_2:+51179762 None:intergenic
TTGTTTCATTCTGTGGAATT+TGG 0.189510 4_2:+51179483 None:intergenic
GAGGCTGATGGGTTGTTGTT+TGG 0.212633 4_2:-51179191 Msa0605370:CDS
TGTATTTCATGGGAGTTTAT+TGG 0.216371 4_2:-51177114 Msa0605370:three_prime_UTR
TTTGATATCTGCTGAGAATT+TGG 0.216664 4_2:-51179216 Msa0605370:CDS
GTTCCTATTGGATATACTTT+TGG 0.228007 4_2:-51177506 Msa0605370:CDS
CCTCTTACGCTGTAGTTTCT+TGG 0.238361 4_2:+51179033 None:intergenic
AGGCTGATGGGTTGTTGTTT+GGG 0.238388 4_2:-51179190 Msa0605370:CDS
TCCGACGAGGTTTGGTAGTA+TGG 0.239808 4_2:-51179165 Msa0605370:CDS
TTGGCACTTGCTGAGTATTA+AGG 0.292384 4_2:-51177386 Msa0605370:exon
AAGCTTGCTGGTGTTCCTGC+TGG 0.298841 4_2:-51179089 Msa0605370:CDS
TTTATGACTCATGTATTTCA+TGG 0.367975 4_2:-51177125 Msa0605370:three_prime_UTR
AATCTTGTTGTGTTTATTGT+TGG 0.373601 4_2:-51177261 Msa0605370:three_prime_UTR
AAAGGGGTTGATTCTGTTGA+TGG 0.375228 4_2:-51179329 Msa0605370:CDS
TTTGAAATGGACTCAATTAG+AGG 0.383271 4_2:-51177473 Msa0605370:CDS
TTGATATTTCAAAATTTCAA+TGG 0.384573 4_2:-51179582 Msa0605370:exon
GGAATGCTCTATGTTCCTAT+TGG 0.388744 4_2:-51177518 Msa0605370:CDS
TGATATTTCAAAATTTCAAT+GGG 0.396895 4_2:-51179581 Msa0605370:exon
AGCTTGCTGGTGTTCCTGCT+GGG 0.399729 4_2:-51179088 Msa0605370:CDS
AACAAATGTTGGATTTATGA+AGG 0.414289 4_2:+51179753 None:intergenic
GGTTCATTCTCTTCTGTAGT+TGG 0.415195 4_2:+51179521 None:intergenic
CTACTTGTTGTTTCATTCTG+TGG 0.416265 4_2:+51179476 None:intergenic
ATTAGTTCCAACACTTCCTT+TGG 0.423053 4_2:+51179269 None:intergenic
TTTGGGTTTCCGACGAGGTT+TGG 0.429899 4_2:-51179173 Msa0605370:CDS
TGGTGCTGGAATGTTTGAAA+TGG 0.434829 4_2:-51177486 Msa0605370:CDS
GCTAATATAGTCAAGAAGTT+AGG 0.438947 4_2:-51177353 Msa0605370:three_prime_UTR
GCACCAAAAGTATATCCAAT+AGG 0.439235 4_2:+51177503 None:intergenic
GAGAATTTGGTGGAGGCTGA+TGG 0.442575 4_2:-51179203 Msa0605370:CDS
TGGAAGGAACAGAAGCTTGC+TGG 0.451653 4_2:-51179101 Msa0605370:CDS
GATTCTGTTGATGGAGTTGA+AGG 0.452417 4_2:-51179320 Msa0605370:CDS
CAATGCAACGTGAAGGTGCT+TGG 0.454677 4_2:+51179867 None:intergenic
GCATAGGTTCCAGCACCATA+TGG 0.464417 4_2:+51177443 None:intergenic
ATTTGGCTTTCAACACTAAG+AGG 0.467974 4_2:+51179500 None:intergenic
CTTGTTGTGTTTATTGTTGG+AGG 0.474131 4_2:-51177258 Msa0605370:three_prime_UTR
TAGTATGGCGGCACAGATGA+AGG 0.480751 4_2:-51179150 Msa0605370:CDS
GATATCTGCTGAGAATTTGG+TGG 0.481344 4_2:-51179213 Msa0605370:CDS
GGAACCTCATCATCTTTCTC+AGG 0.484933 4_2:+51179239 None:intergenic
ATTTCAATGGGGAAAGGAAG+AGG 0.491973 4_2:-51179569 Msa0605370:exon
TCGATTCCACGGGGCAGTTG+TGG 0.493726 4_2:-51179121 Msa0605370:CDS
TATTGTTGGAGGTATAAGAT+TGG 0.496678 4_2:-51177247 Msa0605370:three_prime_UTR
TTTGTTAGCACTGGTACTCA+AGG 0.497633 4_2:-51179062 Msa0605370:CDS
TTCAAAATTTCAATGGGGAA+AGG 0.501971 4_2:-51179575 Msa0605370:exon
TATGGTGCTGGAACCTATGC+AGG 0.505168 4_2:-51177440 Msa0605370:CDS
TCTTGAACCATCTCCTGCAT+AGG 0.505315 4_2:+51177427 None:intergenic
TTATGACTCATGTATTTCAT+GGG 0.510586 4_2:-51177124 Msa0605370:three_prime_UTR
GTATGGTCATGTGGAATCAT+TGG 0.514794 4_2:-51179366 Msa0605370:CDS
ATTAGAGGTGGATCTCCATA+TGG 0.519166 4_2:-51177458 Msa0605370:CDS
AGAATTTGGTGGAGGCTGAT+GGG 0.527716 4_2:-51179202 Msa0605370:CDS
ACAAGCAACTGAAGCTGAGT+TGG 0.528852 4_2:-51177405 Msa0605370:CDS
GTTGGAGGTATAAGATTGGT+AGG 0.533450 4_2:-51177243 Msa0605370:three_prime_UTR
GGTGGATCTCCATATGGTGC+TGG 0.541319 4_2:-51177452 Msa0605370:CDS
GACGCTGCCAAAGGAAGTGT+TGG 0.543102 4_2:-51179276 Msa0605370:CDS
TGTCATTCATTCTCTTCTGG+AGG 0.544657 4_2:-51177209 Msa0605370:three_prime_UTR
TTATTCAATGTATGGTCATG+TGG 0.560406 4_2:-51179375 Msa0605370:CDS
ATCTGCTGAGAATTTGGTGG+AGG 0.570664 4_2:-51179210 Msa0605370:CDS
AAGACAGAGAGGTGGGTGGG+TGG 0.571460 4_2:+51179840 None:intergenic
ATTCTGTTGATGGAGTTGAA+GGG 0.590324 4_2:-51179319 Msa0605370:CDS
AGCACTGGTACTCAAGGTGG+TGG 0.594678 4_2:-51179056 Msa0605370:CDS
TTCCACGGGGCAGTTGTGGA+AGG 0.595264 4_2:-51179117 Msa0605370:CDS
GTTAGCACTGGTACTCAAGG+TGG 0.598234 4_2:-51179059 Msa0605370:CDS
TGTTGTTTGGGTTTCCGACG+AGG 0.600440 4_2:-51179178 Msa0605370:CDS
GAAATGGACTCAATTAGAGG+TGG 0.608938 4_2:-51177470 Msa0605370:CDS
AGTATGGCGGCACAGATGAA+GGG 0.615125 4_2:-51179149 Msa0605370:CDS
TGTCGTAGAGACGCTGCCAA+AGG 0.620906 4_2:-51179285 Msa0605370:CDS
TTCCTTCCACAACTGCCCCG+TGG 0.623823 4_2:+51179115 None:intergenic
CCAGAAGAGAATGAATGACA+AGG 0.626258 4_2:+51177212 None:intergenic
AGATGTAGCAACAATAGATG+TGG 0.630772 4_2:-51179447 Msa0605370:CDS
AGCACCTGAGAAAGATGATG+AGG 0.634435 4_2:-51179243 Msa0605370:CDS
GCTGGAACCTATGCAGGAGA+TGG 0.635685 4_2:-51177434 Msa0605370:CDS
AAATTCTCAGCAGATATCAA+AGG 0.642913 4_2:+51179218 None:intergenic
TAGGAACATAGAGCATTCCA+TGG 0.644524 4_2:+51177522 None:intergenic
GCCATACTACCAAACCTCGT+CGG 0.662442 4_2:+51179164 None:intergenic
GATATTTCAAAATTTCAATG+GGG 0.665834 4_2:-51179580 Msa0605370:exon
GACGAGGTTTGGTAGTATGG+CGG 0.666692 4_2:-51179162 Msa0605370:CDS
TGAGTATTAAGGTAGATACA+TGG 0.679135 4_2:-51177375 Msa0605370:exon
ATAACACAATTAGTCCACCA+TGG 0.694726 4_2:-51177539 Msa0605370:CDS
TGGAAAACAATGCAACGTGA+AGG 0.695980 4_2:+51179860 None:intergenic
GAACATAGAGCATTCCATGG+TGG 0.721594 4_2:+51177525 None:intergenic
TTGGCAGCGTCTCTACGACA+CGG 0.741183 4_2:+51179288 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAATTATAAAAACTTT+AGG + chr4_2:51178029-51178048 None:intergenic 10.0%
!! TAATCATATAAATATTACTA+GGG + chr4_2:51179419-51179438 None:intergenic 10.0%
!! TTAATCATATAAATATTACT+AGG + chr4_2:51179420-51179439 None:intergenic 10.0%
!!! TGAAATTTTGAAATATCAAT+TGG + chr4_2:51177432-51177451 None:intergenic 15.0%
!! TTGATATTTCAAAATTTCAA+TGG - chr4_2:51177433-51177452 Msa0605370:CDS 15.0%
!! TGATATTTCAAAATTTCAAT+GGG - chr4_2:51177434-51177453 Msa0605370:CDS 15.0%
!!! ATTGTTTTTTATTCAATGTA+TGG - chr4_2:51177632-51177651 Msa0605370:intron 15.0%
!! AGGAAAACAAAAAAAATTAA+AGG + chr4_2:51178009-51178028 None:intergenic 15.0%
!! TCATACTAAAAAAAATAGAA+CGG + chr4_2:51178536-51178555 None:intergenic 15.0%
!!! TATTTTTTTTAGTATGAGTA+CGG - chr4_2:51178539-51178558 Msa0605370:intron 15.0%
!!! TGTGATTAAATTTTGTGTAA+TGG + chr4_2:51177229-51177248 None:intergenic 20.0%
!! GTGTATTAAAAAACAAATGT+TGG + chr4_2:51177276-51177295 None:intergenic 20.0%
!! GATATTTCAAAATTTCAATG+GGG - chr4_2:51177435-51177454 Msa0605370:CDS 20.0%
!!! ATATTTTTGTTGTTAGTTGA+AGG + chr4_2:51178289-51178308 None:intergenic 20.0%
!!! TTGTTATTAGTTAAATGTTG+TGG - chr4_2:51178308-51178327 Msa0605370:intron 20.0%
!!! ATTTTTTTTAGTATGAGTAC+GGG - chr4_2:51178540-51178559 Msa0605370:intron 20.0%
! ATGACAAAAAGAAAGAGAAA+AGG + chr4_2:51177204-51177223 None:intergenic 25.0%
! TGGATTTATGAAGGTTAATT+TGG + chr4_2:51177256-51177275 None:intergenic 25.0%
! AACAAATGTTGGATTTATGA+AGG + chr4_2:51177265-51177284 None:intergenic 25.0%
!! TCAATTGGTCAACTATATTT+TGG + chr4_2:51177417-51177436 None:intergenic 25.0%
! ATATAGAAGTTGGAGTAATA+AGG - chr4_2:51178246-51178265 Msa0605370:intron 25.0%
! TATAGACATTTGATACATGT+GGG + chr4_2:51178815-51178834 None:intergenic 25.0%
! ATATAGACATTTGATACATG+TGG + chr4_2:51178816-51178835 None:intergenic 25.0%
! AACTCAAATAGTCAAATTTG+AGG - chr4_2:51178850-51178869 Msa0605370:intron 25.0%
!!! AAAGTGTTTTTAGACTACTT+TGG - chr4_2:51178930-51178949 Msa0605370:intron 25.0%
!! AAGGAAAAAACTTCTACTTT+TGG + chr4_2:51179279-51179298 None:intergenic 25.0%
! AATTTCTATTGTTATGTAGC+TGG - chr4_2:51179447-51179466 Msa0605370:CDS 25.0%
!! AATCTTGTTGTGTTTATTGT+TGG - chr4_2:51179754-51179773 Msa0605370:five_prime_UTR 25.0%
! TTTATGACTCATGTATTTCA+TGG - chr4_2:51179890-51179909 Msa0605370:five_prime_UTR 25.0%
! TTATGACTCATGTATTTCAT+GGG - chr4_2:51179891-51179910 Msa0605370:five_prime_UTR 25.0%
TTCAAAATTTCAATGGGGAA+AGG - chr4_2:51177440-51177459 Msa0605370:CDS 30.0%
TTGTTTCATTCTGTGGAATT+TGG + chr4_2:51177535-51177554 None:intergenic 30.0%
TTATTCAATGTATGGTCATG+TGG - chr4_2:51177640-51177659 Msa0605370:intron 30.0%
TTGGCAAGATCATTGAAAAA+AGG - chr4_2:51177668-51177687 Msa0605370:intron 30.0%
TGGCAAGATCATTGAAAAAA+GGG - chr4_2:51177669-51177688 Msa0605370:intron 30.0%
AAATTCTCAGCAGATATCAA+AGG + chr4_2:51177800-51177819 None:intergenic 30.0%
TTTGATATCTGCTGAGAATT+TGG - chr4_2:51177799-51177818 Msa0605370:intron 30.0%
CACTGTTATTACGAGTAATA+AGG - chr4_2:51178199-51178218 Msa0605370:intron 30.0%
TTACGAGTAATAAGGTGATT+TGG - chr4_2:51178207-51178226 Msa0605370:intron 30.0%
TAACATTAATCCTAAAGTCG+AGG + chr4_2:51178438-51178457 None:intergenic 30.0%
!! CGACTTTAGGATTAATGTTA+TGG - chr4_2:51178438-51178457 Msa0605370:intron 30.0%
ATCTATTCAAAGGTTATGTG+TGG - chr4_2:51178492-51178511 Msa0605370:intron 30.0%
! AGTACGGGTAATAGTTATTT+TGG - chr4_2:51178555-51178574 Msa0605370:intron 30.0%
GACTAATAAAGTGCAAAATC+AGG + chr4_2:51178643-51178662 None:intergenic 30.0%
ATAGACATTTGATACATGTG+GGG + chr4_2:51178814-51178833 None:intergenic 30.0%
!! CTTCTACTCTAAAATTACCT+AGG + chr4_2:51178880-51178899 None:intergenic 30.0%
ACAAGCCAATGAATCAATAT+GGG - chr4_2:51179148-51179167 Msa0605370:CDS 30.0%
TGTGACTAGTAATAGTAACA+TGG - chr4_2:51179214-51179233 Msa0605370:CDS 30.0%
ACATTGAATGCACAATTTCA+AGG + chr4_2:51179298-51179317 None:intergenic 30.0%
! GTTCCTATTGGATATACTTT+TGG - chr4_2:51179509-51179528 Msa0605370:CDS 30.0%
! TTTGAAATGGACTCAATTAG+AGG - chr4_2:51179542-51179561 Msa0605370:CDS 30.0%
TGAGTATTAAGGTAGATACA+TGG - chr4_2:51179640-51179659 Msa0605370:five_prime_UTR 30.0%
GCTAATATAGTCAAGAAGTT+AGG - chr4_2:51179662-51179681 Msa0605370:five_prime_UTR 30.0%
! TATTGTTGGAGGTATAAGAT+TGG - chr4_2:51179768-51179787 Msa0605370:five_prime_UTR 30.0%
TGTATTTCATGGGAGTTTAT+TGG - chr4_2:51179901-51179920 Msa0605370:five_prime_UTR 30.0%
AGAAAAGGAAAAAGACAGAG+AGG + chr4_2:51177189-51177208 None:intergenic 35.0%
!! ATTTGGCTTTCAACACTAAG+AGG + chr4_2:51177518-51177537 None:intergenic 35.0%
!! CTACTTGTTGTTTCATTCTG+TGG + chr4_2:51177542-51177561 None:intergenic 35.0%
AGATGTAGCAACAATAGATG+TGG - chr4_2:51177568-51177587 Msa0605370:intron 35.0%
GGCAAGATCATTGAAAAAAG+GGG - chr4_2:51177670-51177689 Msa0605370:intron 35.0%
! ATTCTGTTGATGGAGTTGAA+GGG - chr4_2:51177696-51177715 Msa0605370:intron 35.0%
ATTAGTTCCAACACTTCCTT+TGG + chr4_2:51177749-51177768 None:intergenic 35.0%
!! AATAAGGTGATTTGGTGGTT+TGG - chr4_2:51178215-51178234 Msa0605370:intron 35.0%
TGTGATTAAACCTCGACTTT+AGG - chr4_2:51178425-51178444 Msa0605370:intron 35.0%
ATTAATGTTATGGCGAACCA+GGG - chr4_2:51178448-51178467 Msa0605370:intron 35.0%
GTCAAATTTGAGGAATACCT+AGG - chr4_2:51178860-51178879 Msa0605370:intron 35.0%
TCATGCCAACCAAATATTTG+TGG - chr4_2:51179026-51179045 Msa0605370:intron 35.0%
ACTGGAGGAACTAATTAGTT+GGG - chr4_2:51179050-51179069 Msa0605370:CDS 35.0%
! GGTATGATTGAAGGACTTTT+TGG - chr4_2:51179091-51179110 Msa0605370:CDS 35.0%
! AAGGACTTTTTGGACTATAG+AGG - chr4_2:51179101-51179120 Msa0605370:CDS 35.0%
!! CATATTGATTCATTGGCTTG+TGG + chr4_2:51179149-51179168 None:intergenic 35.0%
CACAAGCCAATGAATCAATA+TGG - chr4_2:51179147-51179166 Msa0605370:CDS 35.0%
CAAGCCAATGAATCAATATG+GGG - chr4_2:51179149-51179168 Msa0605370:CDS 35.0%
!! AAAATGTTAACTTGTGCCCT+TGG - chr4_2:51179237-51179256 Msa0605370:CDS 35.0%
!! AAATGTTAACTTGTGCCCTT+GGG - chr4_2:51179238-51179257 Msa0605370:CDS 35.0%
ATAACACAATTAGTCCACCA+TGG - chr4_2:51179476-51179495 Msa0605370:CDS 35.0%
! GCACCAAAAGTATATCCAAT+AGG + chr4_2:51179515-51179534 None:intergenic 35.0%
!! ATTGGATATACTTTTGGTGC+TGG - chr4_2:51179515-51179534 Msa0605370:CDS 35.0%
!!! TCAAGAGAAACTAGCTTTTC+TGG + chr4_2:51179688-51179707 None:intergenic 35.0%
! CTTGTTGTGTTTATTGTTGG+AGG - chr4_2:51179757-51179776 Msa0605370:five_prime_UTR 35.0%
TGGAAAACAATGCAACGTGA+AGG + chr4_2:51177158-51177177 None:intergenic 40.0%
AAGGAAAAAGACAGAGAGGT+GGG + chr4_2:51177185-51177204 None:intergenic 40.0%
AAAGGAAAAAGACAGAGAGG+TGG + chr4_2:51177186-51177205 None:intergenic 40.0%
ATTTCAATGGGGAAAGGAAG+AGG - chr4_2:51177446-51177465 Msa0605370:CDS 40.0%
!!! GTTGGTGCTTTTTTCTTGCT+TGG + chr4_2:51177479-51177498 None:intergenic 40.0%
GGTTCATTCTCTTCTGTAGT+TGG + chr4_2:51177497-51177516 None:intergenic 40.0%
GTATGGTCATGTGGAATCAT+TGG - chr4_2:51177649-51177668 Msa0605370:intron 40.0%
! AAAGGGGTTGATTCTGTTGA+TGG - chr4_2:51177686-51177705 Msa0605370:intron 40.0%
! GATTCTGTTGATGGAGTTGA+AGG - chr4_2:51177695-51177714 Msa0605370:intron 40.0%
GATATCTGCTGAGAATTTGG+TGG - chr4_2:51177802-51177821 Msa0605370:intron 40.0%
!! TTTGTTAGCACTGGTACTCA+AGG - chr4_2:51177953-51177972 Msa0605370:intron 40.0%
CAAGAAACTACAGCGTAAGA+GGG - chr4_2:51177983-51178002 Msa0605370:intron 40.0%
! CGAGTAATAAGGTGATTTGG+TGG - chr4_2:51178210-51178229 Msa0605370:intron 40.0%
GGAAGGAGGAATATAGAAGT+TGG - chr4_2:51178236-51178255 Msa0605370:intron 40.0%
!!! CCTCTGATGAGTTTGTTTTG+TGG + chr4_2:51178387-51178406 None:intergenic 40.0%
CCACAAAACAAACTCATCAG+AGG - chr4_2:51178384-51178403 Msa0605370:intron 40.0%
GATTAATGTTATGGCGAACC+AGG - chr4_2:51178447-51178466 Msa0605370:intron 40.0%
TTAATGTTATGGCGAACCAG+GGG - chr4_2:51178449-51178468 Msa0605370:intron 40.0%
AATGTCTATATCCGTCCATC+TGG - chr4_2:51178825-51178844 Msa0605370:intron 40.0%
CAGTGCCACAAATATTTGGT+TGG + chr4_2:51179034-51179053 None:intergenic 40.0%
CAACCAAATATTTGTGGCAC+TGG - chr4_2:51179032-51179051 Msa0605370:intron 40.0%
CACTGGAGGAACTAATTAGT+TGG - chr4_2:51179049-51179068 Msa0605370:CDS 40.0%
CTGGAGGAACTAATTAGTTG+GGG - chr4_2:51179051-51179070 Msa0605370:CDS 40.0%
TGGAGGAACTAATTAGTTGG+GGG - chr4_2:51179052-51179071 Msa0605370:CDS 40.0%
GAACTAATTAGTTGGGGGTA+GGG - chr4_2:51179057-51179076 Msa0605370:CDS 40.0%
AACTAATTAGTTGGGGGTAG+GGG - chr4_2:51179058-51179077 Msa0605370:CDS 40.0%
!! CCTACCCCATATTGATTCAT+TGG + chr4_2:51179156-51179175 None:intergenic 40.0%
CCAATGAATCAATATGGGGT+AGG - chr4_2:51179153-51179172 Msa0605370:CDS 40.0%
!! AATGTTAACTTGTGCCCTTG+GGG - chr4_2:51179239-51179258 Msa0605370:CDS 40.0%
TTCCTTAACTTATGCCCCAA+GGG + chr4_2:51179256-51179275 None:intergenic 40.0%
!! TTCCTTAACTTGTGCCCTAA+GGG - chr4_2:51179378-51179397 Msa0605370:CDS 40.0%
!! CATGTTAACTTGTGCCCTTA+GGG + chr4_2:51179395-51179414 None:intergenic 40.0%
!! TCATGTTAACTTGTGCCCTT+AGG + chr4_2:51179396-51179415 None:intergenic 40.0%
! TAGGAACATAGAGCATTCCA+TGG + chr4_2:51179496-51179515 None:intergenic 40.0%
GGAATGCTCTATGTTCCTAT+TGG - chr4_2:51179497-51179516 Msa0605370:CDS 40.0%
! TGGTGCTGGAATGTTTGAAA+TGG - chr4_2:51179529-51179548 Msa0605370:CDS 40.0%
! GAAATGGACTCAATTAGAGG+TGG - chr4_2:51179545-51179564 Msa0605370:CDS 40.0%
ATTAGAGGTGGATCTCCATA+TGG - chr4_2:51179557-51179576 Msa0605370:CDS 40.0%
! TTGGCACTTGCTGAGTATTA+AGG - chr4_2:51179629-51179648 Msa0605370:five_prime_UTR 40.0%
! GTTGGAGGTATAAGATTGGT+AGG - chr4_2:51179772-51179791 Msa0605370:five_prime_UTR 40.0%
CCAGAAGAGAATGAATGACA+AGG + chr4_2:51179806-51179825 None:intergenic 40.0%
CCTTGTCATTCATTCTCTTC+TGG - chr4_2:51179803-51179822 Msa0605370:five_prime_UTR 40.0%
TGTCATTCATTCTCTTCTGG+AGG - chr4_2:51179806-51179825 Msa0605370:five_prime_UTR 40.0%
AAAAAGACAGAGAGGTGGGT+GGG + chr4_2:51177181-51177200 None:intergenic 45.0%
AGCACCTGAGAAAGATGATG+AGG - chr4_2:51177772-51177791 Msa0605370:intron 45.0%
GGAACCTCATCATCTTTCTC+AGG + chr4_2:51177779-51177798 None:intergenic 45.0%
! ATCTGCTGAGAATTTGGTGG+AGG - chr4_2:51177805-51177824 Msa0605370:intron 45.0%
! AGAATTTGGTGGAGGCTGAT+GGG - chr4_2:51177813-51177832 Msa0605370:intron 45.0%
!! AGGCTGATGGGTTGTTGTTT+GGG - chr4_2:51177825-51177844 Msa0605370:intron 45.0%
! GAAGGGACTTTTCGATTCCA+CGG - chr4_2:51177883-51177902 Msa0605370:intron 45.0%
! AAGGGACTTTTCGATTCCAC+GGG - chr4_2:51177884-51177903 Msa0605370:intron 45.0%
GTGCTAACAAAAAACCCAGC+AGG + chr4_2:51177944-51177963 None:intergenic 45.0%
!!! GCTGGGTTTTTTGTTAGCAC+TGG - chr4_2:51177944-51177963 Msa0605370:intron 45.0%
CCTCTTACGCTGTAGTTTCT+TGG + chr4_2:51177985-51178004 None:intergenic 45.0%
CCAAGAAACTACAGCGTAAG+AGG - chr4_2:51177982-51178001 Msa0605370:intron 45.0%
!! AGGTGATTTGGTGGTTTGGA+AGG - chr4_2:51178219-51178238 Msa0605370:intron 45.0%
!! TGATTTGGTGGTTTGGAAGG+AGG - chr4_2:51178222-51178241 Msa0605370:intron 45.0%
TGTGGCTCAATTGACAAGAC+AGG - chr4_2:51178326-51178345 Msa0605370:intron 45.0%
! ACAAGACAGGTTGAGCTTGT+GGG - chr4_2:51178339-51178358 Msa0605370:intron 45.0%
CAAAGGGCGGATCTATTCAA+AGG - chr4_2:51178482-51178501 Msa0605370:intron 45.0%
! ATTTGAGTTCGCCAGATGGA+CGG + chr4_2:51178839-51178858 None:intergenic 45.0%
! GACTATTTGAGTTCGCCAGA+TGG + chr4_2:51178843-51178862 None:intergenic 45.0%
TACGATACCACATGTGCTTG+TGG - chr4_2:51178997-51179016 Msa0605370:intron 45.0%
ACACAAACCACAAGCACATG+TGG + chr4_2:51179007-51179026 None:intergenic 45.0%
CCTCCAGTGCCACAAATATT+TGG + chr4_2:51179038-51179057 None:intergenic 45.0%
! CCAAATATTTGTGGCACTGG+AGG - chr4_2:51179035-51179054 Msa0605370:intron 45.0%
GGAACTAATTAGTTGGGGGT+AGG - chr4_2:51179056-51179075 Msa0605370:CDS 45.0%
! CAATGGTGGGGTATGATTGA+AGG - chr4_2:51179082-51179101 Msa0605370:CDS 45.0%
GTTCCTTAACTTATGCCCCA+AGG + chr4_2:51179257-51179276 None:intergenic 45.0%
!! GTTCCTTAACTTGTGCCCTA+AGG - chr4_2:51179377-51179396 Msa0605370:CDS 45.0%
! GAACATAGAGCATTCCATGG+TGG + chr4_2:51179493-51179512 None:intergenic 45.0%
TCTTGAACCATCTCCTGCAT+AGG + chr4_2:51179591-51179610 None:intergenic 45.0%
ACAAGCAACTGAAGCTGAGT+TGG - chr4_2:51179610-51179629 Msa0605370:five_prime_UTR 45.0%
!! CAATGCAACGTGAAGGTGCT+TGG + chr4_2:51177151-51177170 None:intergenic 50.0%
GAAAAAGACAGAGAGGTGGG+TGG + chr4_2:51177182-51177201 None:intergenic 50.0%
! GAGAATTTGGTGGAGGCTGA+TGG - chr4_2:51177812-51177831 Msa0605370:intron 50.0%
!! GAGGCTGATGGGTTGTTGTT+TGG - chr4_2:51177824-51177843 Msa0605370:intron 50.0%
! TGTTGTTTGGGTTTCCGACG+AGG - chr4_2:51177837-51177856 Msa0605370:intron 50.0%
! TTTGGGTTTCCGACGAGGTT+TGG - chr4_2:51177842-51177861 Msa0605370:intron 50.0%
! TCCGACGAGGTTTGGTAGTA+TGG - chr4_2:51177850-51177869 Msa0605370:intron 50.0%
GCCATACTACCAAACCTCGT+CGG + chr4_2:51177854-51177873 None:intergenic 50.0%
! GACGAGGTTTGGTAGTATGG+CGG - chr4_2:51177853-51177872 Msa0605370:intron 50.0%
TAGTATGGCGGCACAGATGA+AGG - chr4_2:51177865-51177884 Msa0605370:intron 50.0%
AGTATGGCGGCACAGATGAA+GGG - chr4_2:51177866-51177885 Msa0605370:intron 50.0%
! AGGGACTTTTCGATTCCACG+GGG - chr4_2:51177885-51177904 Msa0605370:intron 50.0%
TGGAAGGAACAGAAGCTTGC+TGG - chr4_2:51177914-51177933 Msa0605370:intron 50.0%
!! GTTAGCACTGGTACTCAAGG+TGG - chr4_2:51177956-51177975 Msa0605370:intron 50.0%
! GACAAGACAGGTTGAGCTTG+TGG - chr4_2:51178338-51178357 Msa0605370:intron 50.0%
CAGGGGCGGATCTATTCAAA+GGG - chr4_2:51178466-51178485 Msa0605370:intron 50.0%
TGCCCTTGGGGCATAAGTTA+AGG - chr4_2:51179251-51179270 Msa0605370:CDS 50.0%
TGCCCTTAGGGCACAAGTTA+AGG + chr4_2:51179383-51179402 None:intergenic 50.0%
GCATAGGTTCCAGCACCATA+TGG + chr4_2:51179575-51179594 None:intergenic 50.0%
! TATGGTGCTGGAACCTATGC+AGG - chr4_2:51179575-51179594 Msa0605370:exon 50.0%
TTGGCAGCGTCTCTACGACA+CGG + chr4_2:51177730-51177749 None:intergenic 55.0%
TGTCGTAGAGACGCTGCCAA+AGG - chr4_2:51177730-51177749 Msa0605370:intron 55.0%
! GACGCTGCCAAAGGAAGTGT+TGG - chr4_2:51177739-51177758 Msa0605370:intron 55.0%
!! AAGCTTGCTGGTGTTCCTGC+TGG - chr4_2:51177926-51177945 Msa0605370:intron 55.0%
!! AGCTTGCTGGTGTTCCTGCT+GGG - chr4_2:51177927-51177946 Msa0605370:intron 55.0%
!! AGCACTGGTACTCAAGGTGG+TGG - chr4_2:51177959-51177978 Msa0605370:intron 55.0%
AGGTTGAGCTTGTGGGTGAG+AGG - chr4_2:51178346-51178365 Msa0605370:intron 55.0%
ATGTTATGGCGAACCAGGGG+CGG - chr4_2:51178452-51178471 Msa0605370:intron 55.0%
CCTTTGAATAGATCCGCCCC+TGG + chr4_2:51178468-51178487 None:intergenic 55.0%
CCAGGGGCGGATCTATTCAA+AGG - chr4_2:51178465-51178484 Msa0605370:intron 55.0%
GGGCGGATCTATTCAAAGGG+CGG - chr4_2:51178469-51178488 Msa0605370:intron 55.0%
TAGTTGGGGGTAGGGGTCAA+TGG - chr4_2:51179065-51179084 Msa0605370:CDS 55.0%
GGTGGATCTCCATATGGTGC+TGG - chr4_2:51179563-51179582 Msa0605370:CDS 55.0%
GCTGGAACCTATGCAGGAGA+TGG - chr4_2:51179581-51179600 Msa0605370:exon 55.0%
AAGACAGAGAGGTGGGTGGG+TGG + chr4_2:51177178-51177197 None:intergenic 60.0%
TCGATTCCACGGGGCAGTTG+TGG - chr4_2:51177894-51177913 Msa0605370:intron 60.0%
TTCCACGGGGCAGTTGTGGA+AGG - chr4_2:51177898-51177917 Msa0605370:intron 60.0%
TTCCTTCCACAACTGCCCCG+TGG + chr4_2:51177903-51177922 None:intergenic 60.0%
TTGGGGGTAGGGGTCAATGG+TGG - chr4_2:51179068-51179087 Msa0605370:CDS 60.0%
TGGGGGTAGGGGTCAATGGT+GGG - chr4_2:51179069-51179088 Msa0605370:CDS 60.0%
GGGGGTAGGGGTCAATGGTG+GGG - chr4_2:51179070-51179089 Msa0605370:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 51177106 51179931 51177106 ID=Msa0605370;Name=Msa0605370
chr4_2 mRNA 51177106 51179931 51177106 ID=Msa0605370-mRNA-1;Parent=Msa0605370;Name=Msa0605370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=346|1|1|1|1|1|2|282|242
chr4_2 exon 51177106 51177571 51177106 ID=Msa0605370-mRNA-1:exon:12216;Parent=Msa0605370-mRNA-1
chr4_2 exon 51179041 51179931 51179041 ID=Msa0605370-mRNA-1:exon:12215;Parent=Msa0605370-mRNA-1
chr4_2 five_prime_UTR 51179586 51179931 51179586 ID=Msa0605370-mRNA-1:five_prime_utr;Parent=Msa0605370-mRNA-1
chr4_2 CDS 51179041 51179585 51179041 ID=Msa0605370-mRNA-1:cds;Parent=Msa0605370-mRNA-1
chr4_2 CDS 51177388 51177571 51177388 ID=Msa0605370-mRNA-1:cds;Parent=Msa0605370-mRNA-1
chr4_2 three_prime_UTR 51177106 51177387 51177106 ID=Msa0605370-mRNA-1:three_prime_utr;Parent=Msa0605370-mRNA-1
Gene Sequence

>Msa0605370

ATGGGGAAAGGAAGAGGTTGCATACCAAGCAAGAAAAAAGCACCAACTACAGAAGAGAATGAACCTCTTAGTGTTGAAAGCCAAATTCCACAGAATGAAACAACAAGTAGAAGCATAGATGTAGCAACAATAGATGTGGCAACAACATCAGAAGAAGCAGTGAAAAAGCTCAAAGTTTACATTGTTTTTTATTCAATGTATGGTCATGTGGAATCATTGGCAAGATCATTGAAAAAAGGGGTTGATTCTGTTGATGGAGTTGAAGGGTTTTTGTACCGTGTCGTAGAGACGCTGCCAAAGGAAGTGTTGGAACTAATGAAAGCACCTGAGAAAGATGATGAGGTTCCTTTGATATCTGCTGAGAATTTGGTGGAGGCTGATGGGTTGTTGTTTGGGTTTCCGACGAGGTTTGGTAGTATGGCGGCACAGATGAAGGGACTTTTCGATTCCACGGGGCAGTTGTGGAAGGAACAGAAGCTTGCTGGTGTTCCTGCTGGGTTTTTTGTTAGCACTGGTACTCAAGGTGGTGGCCAAGAAACTACAGCCTGGACAGCAATAACACAATTAGTCCACCATGGAATGCTCTATGTTCCTATTGGATATACTTTTGGTGCTGGAATGTTTGAAATGGACTCAATTAGAGGTGGATCTCCATATGGTGCTGGAACCTATGCAGGAGATGGTTCAAGACAAGCAACTGAAGCTGAGTTGGCACTTGCTGAGTATTAA
Protein sequence

>Msa0605370

MGKGRGCIPSKKKAPTTEENEPLSVESQIPQNETTSRSIDVATIDVATTSEEAVKKLKVYIVFYSMYGHVESLARSLKKGVDSVDGVEGFLYRVVETLPKEVLELMKAPEKDDEVPLISAENLVEADGLLFGFPTRFGSMAAQMKGLFDSTGQLWKEQKLAGVPAGFFVSTGTQGGGQETTAWTAITQLVHHGMLYVPIGYTFGAGMFEMDSIRGGSPYGAGTYAGDGSRQATEAELALAEY*