Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0731560 | Msa0768290 | 0.820091 | 8.235717e-53 | -8.615850e-47 |
| Msa0731560 | Msa0768370 | 0.823052 | 1.704934e-53 | -8.615850e-47 |
| Msa0731560 | Msa0783160 | 0.800295 | 1.541337e-48 | -8.615850e-47 |
| Msa0731560 | Msa0833290 | 0.819122 | 1.370851e-52 | -8.615850e-47 |
| Msa0731560 | Msa0888370 | 0.804232 | 2.384083e-49 | -8.615850e-47 |
| Msa0731560 | Msa0899430 | 0.803655 | 3.143149e-49 | -8.615850e-47 |
| Msa0731560 | Msa0956270 | 0.809896 | 1.508790e-50 | -8.615850e-47 |
| Msa0731560 | Msa1015490 | 0.802909 | 4.484331e-49 | -8.615850e-47 |
| Msa0731560 | Msa1016280 | 0.827702 | 1.351914e-54 | -8.615850e-47 |
| Msa0731560 | Msa1025360 | 0.820998 | 5.099939e-53 | -8.615850e-47 |
| Msa0731560 | Msa1025370 | 0.822402 | 2.414443e-53 | -8.615850e-47 |
| Msa0731560 | Msa1044830 | 0.824187 | 9.246100e-54 | -8.615850e-47 |
| Msa0731560 | Msa1044840 | 0.824996 | 5.961214e-54 | -8.615850e-47 |
| Msa0731560 | Msa1060840 | 0.833891 | 4.108647e-56 | -8.615850e-47 |
| Msa0731560 | Msa1109440 | 0.822623 | 2.145396e-53 | -8.615850e-47 |
| Msa0731560 | Msa1121200 | 0.829086 | 6.265732e-55 | -8.615850e-47 |
| Msa0731560 | Msa1149360 | 0.816395 | 5.655325e-52 | -8.615850e-47 |
| Msa0731560 | Msa1150370 | 0.821475 | 3.958191e-53 | -8.615850e-47 |
| Msa0731560 | Msa1184420 | 0.815220 | 1.033786e-51 | -8.615850e-47 |
| Msa0731560 | Msa1192640 | 0.801202 | 1.006418e-48 | -8.615850e-47 |
| Msa0731560 | Msa1230430 | 0.800962 | 1.126475e-48 | -8.615850e-47 |
| Msa0731560 | Msa1285930 | 0.813596 | 2.362297e-51 | -8.615850e-47 |
| Msa0731560 | Msa1287800 | 0.802355 | 5.836223e-49 | -8.615850e-47 |
| Msa0731560 | Msa1287810 | 0.819368 | 1.204913e-52 | -8.615850e-47 |
| Msa0731560 | Msa1289430 | 0.816603 | 5.077872e-52 | -8.615850e-47 |
| Msa0731560 | Msa1415310 | 0.804279 | 2.331603e-49 | -8.615850e-47 |
| Msa0153910 | Msa0731560 | 0.803212 | 3.882391e-49 | -8.615850e-47 |
| Msa0231000 | Msa0731560 | 0.808794 | 2.599554e-50 | -8.615850e-47 |
| Msa0414420 | Msa0731560 | 0.811540 | 6.652260e-51 | -8.615850e-47 |
| Msa0450000 | Msa0731560 | 0.831739 | 1.407507e-55 | -8.615850e-47 |
| Msa0452900 | Msa0731560 | 0.818783 | 1.637170e-52 | -8.615850e-47 |
| Msa0267000 | Msa0731560 | 0.823467 | 1.363716e-53 | -8.615850e-47 |
| Msa0267010 | Msa0731560 | 0.830086 | 3.579550e-55 | -8.615850e-47 |
| Msa0491590 | Msa0731560 | 0.803093 | 4.108435e-49 | -8.615850e-47 |
| Msa0534540 | Msa0731560 | 0.847312 | 1.252603e-59 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGTGTGAAGAGGGAATTC+AGG | 0.319380 | 5_1:-42555162 | Msa0731540:intron |
| GAATTCAGGGGCTGAGTATT+GGG | 0.345428 | 5_1:-42555148 | Msa0731540:intron |
| GGGTTGGAGGGTTGGAAAGG+AGG | 0.358954 | 5_1:-42555232 | Msa0731540:intron |
| AGAAGGGGTGCTCTCAATAT+TGG | 0.373740 | 5_1:-42555112 | Msa0731540:intron |
| GGAATTCAGGGGCTGAGTAT+TGG | 0.409779 | 5_1:-42555149 | Msa0731540:intron |
| AGGAGGTGAGGTGATTATAA+AGG | 0.417595 | 5_1:-42555215 | Msa0731540:intron |
| CAAATGAACTAGTGACCCTT+TGG | 0.418953 | 5_1:-42555255 | Msa0731540:intron |
| AAATGAACTAGTGACCCTTT+GGG | 0.444117 | 5_1:-42555254 | Msa0731540:intron |
| TGAGAGCACCCCTTCTATGA+TGG | 0.473869 | 5_1:+42555119 | Msa0731560:CDS |
| GAGATGAATCGCCTTGCAAA+AGG | 0.483832 | 5_1:+42555073 | Msa0731560:CDS |
| TGGGTGATTCCCATCATAGA+AGG | 0.512742 | 5_1:-42555129 | Msa0731540:intron |
| GAGTGTGAAGAGGGAATTCA+GGG | 0.518513 | 5_1:-42555161 | Msa0731540:intron |
| TACAATATGAATAAAACAGA+TGG | 0.523245 | 5_1:+42555051 | Msa0731560:CDS |
| GGGTGATTCCCATCATAGAA+GGG | 0.553494 | 5_1:-42555128 | Msa0731540:intron |
| GAGAGCACCCCTTCTATGAT+GGG | 0.559574 | 5_1:+42555120 | Msa0731560:CDS |
| AATGAACTAGTGACCCTTTG+GGG | 0.560742 | 5_1:-42555253 | Msa0731540:intron |
| ATGATGGATGAGTGTGAAGA+GGG | 0.572984 | 5_1:-42555170 | Msa0731540:intron |
| GGAGGGTTGGAAAGGAGGTG+AGG | 0.587174 | 5_1:-42555227 | Msa0731540:intron |
| GGTGATTATAAAGGATGTGA+GGG | 0.604984 | 5_1:-42555206 | Msa0731540:intron |
| AGGTGATTATAAAGGATGTG+AGG | 0.621086 | 5_1:-42555207 | Msa0731540:intron |
| TATGATGGATGAGTGTGAAG+AGG | 0.629096 | 5_1:-42555171 | Msa0731540:intron |
| GGTGATTCCCATCATAGAAG+GGG | 0.659237 | 5_1:-42555127 | Msa0731540:intron |
| ATGAACTAGTGACCCTTTGG+GGG | 0.669898 | 5_1:-42555252 | Msa0731540:intron |
| GTGATTATAAAGGATGTGAG+GGG | 0.670074 | 5_1:-42555205 | Msa0731540:intron |
| TGATTATAAAGGATGTGAGG+GGG | 0.681609 | 5_1:-42555204 | Msa0731540:intron |
| AGTGTGAAGAGGGAATTCAG+GGG | 0.805933 | 5_1:-42555160 | Msa0731540:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTTATTCATATTGTATAG+AGG | - | chr5_1:42555048-42555067 | Msa0731540:intron | 20.0% |
| !! | TACAATATGAATAAAACAGA+TGG | + | chr5_1:42555051-42555070 | Msa0731560:CDS | 20.0% |
| ! | TCATATACTTTCCTTTTGCA+AGG | - | chr5_1:42555087-42555106 | Msa0731540:intron | 30.0% |
| TGATTATAAAGGATGTGAGG+GGG | - | chr5_1:42555207-42555226 | Msa0731540:intron | 35.0% | |
| GTGATTATAAAGGATGTGAG+GGG | - | chr5_1:42555208-42555227 | Msa0731540:intron | 35.0% | |
| GGTGATTATAAAGGATGTGA+GGG | - | chr5_1:42555209-42555228 | Msa0731540:intron | 35.0% | |
| AGGTGATTATAAAGGATGTG+AGG | - | chr5_1:42555210-42555229 | Msa0731540:intron | 35.0% | |
| !! | ATGATGGATGAGTGTGAAGA+GGG | - | chr5_1:42555173-42555192 | Msa0731540:intron | 40.0% |
| !! | TATGATGGATGAGTGTGAAG+AGG | - | chr5_1:42555174-42555193 | Msa0731540:intron | 40.0% |
| AGGAGGTGAGGTGATTATAA+AGG | - | chr5_1:42555218-42555237 | Msa0731540:intron | 40.0% | |
| GAGATGAATCGCCTTGCAAA+AGG | + | chr5_1:42555073-42555092 | Msa0731560:CDS | 45.0% | |
| !! | AGAAGGGGTGCTCTCAATAT+TGG | - | chr5_1:42555115-42555134 | Msa0731540:intron | 45.0% |
| GGTGATTCCCATCATAGAAG+GGG | - | chr5_1:42555130-42555149 | Msa0731540:intron | 45.0% | |
| GGGTGATTCCCATCATAGAA+GGG | - | chr5_1:42555131-42555150 | Msa0731540:intron | 45.0% | |
| TGGGTGATTCCCATCATAGA+AGG | - | chr5_1:42555132-42555151 | Msa0731540:intron | 45.0% | |
| GAATTCAGGGGCTGAGTATT+GGG | - | chr5_1:42555151-42555170 | Msa0731540:intron | 45.0% | |
| AGTGTGAAGAGGGAATTCAG+GGG | - | chr5_1:42555163-42555182 | Msa0731540:intron | 45.0% | |
| GAGTGTGAAGAGGGAATTCA+GGG | - | chr5_1:42555164-42555183 | Msa0731540:intron | 45.0% | |
| TGAGTGTGAAGAGGGAATTC+AGG | - | chr5_1:42555165-42555184 | Msa0731540:intron | 45.0% | |
| !! | TGAGAGCACCCCTTCTATGA+TGG | + | chr5_1:42555119-42555138 | Msa0731560:CDS | 50.0% |
| !! | GAGAGCACCCCTTCTATGAT+GGG | + | chr5_1:42555120-42555139 | Msa0731560:CDS | 50.0% |
| GGAATTCAGGGGCTGAGTAT+TGG | - | chr5_1:42555152-42555171 | Msa0731540:intron | 50.0% | |
| AGGATGTGAGGGGGAGCTGA+GGG | - | chr5_1:42555198-42555217 | Msa0731540:intron | 60.0% | |
| AAGGATGTGAGGGGGAGCTG+AGG | - | chr5_1:42555199-42555218 | Msa0731540:intron | 60.0% | |
| GGAGGGTTGGAAAGGAGGTG+AGG | - | chr5_1:42555230-42555249 | Msa0731540:intron | 60.0% | |
| GGGTTGGAGGGTTGGAAAGG+AGG | - | chr5_1:42555235-42555254 | Msa0731540:intron | 60.0% | |
| TGGGGGTTGGAGGGTTGGAA+AGG | - | chr5_1:42555238-42555257 | Msa0731540:intron | 60.0% | |
| TCCAACCCTCCAACCCCCAA+AGG | + | chr5_1:42555239-42555258 | Msa0731560:CDS | 60.0% | |
| CCAACCCTCCAACCCCCAAA+GGG | + | chr5_1:42555240-42555259 | Msa0731560:CDS | 60.0% | |
| ! | GTGACCCTTTGGGGGTTGGA+GGG | - | chr5_1:42555247-42555266 | Msa0731540:intron | 60.0% |
| ! | AGTGACCCTTTGGGGGTTGG+AGG | - | chr5_1:42555248-42555267 | Msa0731540:intron | 60.0% |
| ! | GGGGGAGCTGAGGGGTATGA+TGG | - | chr5_1:42555189-42555208 | Msa0731540:intron | 65.0% |
| GGATGTGAGGGGGAGCTGAG+GGG | - | chr5_1:42555197-42555216 | Msa0731540:intron | 65.0% | |
| ! | CCCTTTGGGGGTTGGAGGGT+TGG | - | chr5_1:42555243-42555262 | Msa0731540:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 42555039 | 42555269 | 42555039 | ID=Msa0731560;Name=Msa0731560 |
| chr5_1 | mRNA | 42555039 | 42555269 | 42555039 | ID=Msa0731560-mRNA-1;Parent=Msa0731560;Name=Msa0731560-mRNA-1;_AED=0.39;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|76 |
| chr5_1 | exon | 42555039 | 42555269 | 42555039 | ID=Msa0731560-mRNA-1:exon:12269;Parent=Msa0731560-mRNA-1 |
| chr5_1 | CDS | 42555039 | 42555269 | 42555039 | ID=Msa0731560-mRNA-1:cds;Parent=Msa0731560-mRNA-1 |
| Gene Sequence |
| Protein sequence |
