AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1149360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1149360 MtrunA17_Chr1g0172351 76.812 69 16 0 99 167 77 145 4.47e-35 120
Msa1149360 MtrunA17_Chr4g0044671 25.000 208 113 1 1 165 1 208 7.86e-21 85.1
Msa1149360 MtrunA17_Chr1g0175581 50.000 78 39 0 88 165 29 106 4.66e-20 80.1
Msa1149360 MtrunA17_Chr4g0034781 25.000 204 110 1 7 167 1 204 6.77e-19 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGGTTGGACTGAGTTTA+AGG 0.185372 7_4:+30618727 Msa1149360:CDS
GGAAAACTTACAACACAATT+TGG 0.198179 7_4:+30619137 Msa1149360:CDS
CAGCAAGTTGATGACGAATT+TGG 0.199887 7_4:+30619023 Msa1149360:intron
TGGCAATGAAGAGAAATTTA+AGG 0.284538 7_4:+30619097 Msa1149360:CDS
GGAATGTATCTCAAGCAATA+TGG 0.328623 7_4:+30619044 Msa1149360:CDS
ATAAAACTTTGTGCTGATAA+TGG 0.338821 7_4:+30619077 Msa1149360:CDS
TAGCTAATCTATCACGTTCA+TGG 0.343597 7_4:+30618633 Msa1149360:CDS
TCAATTTGAAGCGTATTGTT+TGG 0.372308 7_4:-30619205 None:intergenic
AAGGTGTATTTACTTAATGA+TGG 0.416212 7_4:+30619116 Msa1149360:CDS
TCTCAAGCAATATGGTTTCC+AGG 0.423438 7_4:+30619052 Msa1149360:CDS
TTGGCCAACTCTGAAGTTGT+TGG 0.427754 7_4:-30619186 None:intergenic
AAAACAATTTCAACATTGTC+AGG 0.431374 7_4:-30618764 None:intergenic
AGTTGTCAAAGTTGCAGAGC+AGG 0.438195 7_4:+30618461 Msa1149360:CDS
AAATTTGTCTTCACAAAAGC+TGG 0.439036 7_4:-30618611 None:intergenic
CTACGGTAATAATATGTTCT+CGG 0.473564 7_4:+30618793 Msa1149360:CDS
TTTAACTTTCAACAAAAGCT+TGG 0.486750 7_4:+30618688 Msa1149360:CDS
CAACACAATTTGGAATCCTG+TGG 0.534831 7_4:+30619147 Msa1149360:CDS
ACCCCATGTTAACACATGGT+TGG 0.537028 7_4:+30618714 Msa1149360:CDS
AGTCCAACCATGTGTTAACA+TGG 0.542552 7_4:-30618717 None:intergenic
AACACAATTTGGAATCCTGT+GGG 0.587367 7_4:+30619148 Msa1149360:CDS
GTCCAACCATGTGTTAACAT+GGG 0.594446 7_4:-30618716 None:intergenic
TCTTGCAGAAATGATCCCAC+AGG 0.594556 7_4:-30619163 None:intergenic
AAGACCAACAACTTCAGAGT+TGG 0.597015 7_4:+30619182 Msa1149360:CDS
GTTAACCCCATGTTAACACA+TGG 0.616537 7_4:+30618710 Msa1149360:CDS
CAACTGAGACATATTCAAGC+GGG 0.630494 7_4:-30618443 None:intergenic
TCCAACCATGTGTTAACATG+GGG 0.650672 7_4:-30618715 None:intergenic
ACAACTGAGACATATTCAAG+CGG 0.674713 7_4:-30618444 None:intergenic
CTTCTCAATACAGAAAACAC+AGG 0.686274 7_4:+30618659 Msa1149360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGGAAAATAAAACATT+GGG - chr7_4:30618937-30618956 None:intergenic 15.0%
!! AAAAAAGGAAAATAAAACAT+TGG - chr7_4:30618938-30618957 None:intergenic 15.0%
!!! ATTTTAAAAGAAGTGAAAAA+AGG - chr7_4:30618953-30618972 None:intergenic 15.0%
!! AAAAAGGAAAATAAAACATT+GGG - chr7_4:30618937-30618956 None:intergenic 15.0%
!! AAAAAAGGAAAATAAAACAT+TGG - chr7_4:30618938-30618957 None:intergenic 15.0%
!!! ATTTTAAAAGAAGTGAAAAA+AGG - chr7_4:30618953-30618972 None:intergenic 15.0%
!! TTTAACTTTCAACAAAAGCT+TGG + chr7_4:30618688-30618707 Msa1149360:CDS 25.0%
! AAAACAATTTCAACATTGTC+AGG - chr7_4:30618767-30618786 None:intergenic 25.0%
!! GACAATGTTGAAATTGTTTT+TGG + chr7_4:30618767-30618786 Msa1149360:CDS 25.0%
!!! GAAATTGTTTTTGGTTACTA+CGG + chr7_4:30618776-30618795 Msa1149360:CDS 25.0%
! AATGTTAAATGAAGAAGGAA+TGG - chr7_4:30618980-30618999 None:intergenic 25.0%
! GGATAAATGTTAAATGAAGA+AGG - chr7_4:30618985-30619004 None:intergenic 25.0%
! TCATTTAACATTTATCCGAT+TGG + chr7_4:30618988-30619007 Msa1149360:intron 25.0%
! CTGTATAAAACAATACCAAT+CGG - chr7_4:30619006-30619025 None:intergenic 25.0%
!! ATAAAACTTTGTGCTGATAA+TGG + chr7_4:30619077-30619096 Msa1149360:CDS 25.0%
! AAGGTGTATTTACTTAATGA+TGG + chr7_4:30619116-30619135 Msa1149360:CDS 25.0%
!! TTTAACTTTCAACAAAAGCT+TGG + chr7_4:30618688-30618707 Msa1149360:CDS 25.0%
! AAAACAATTTCAACATTGTC+AGG - chr7_4:30618767-30618786 None:intergenic 25.0%
!! GACAATGTTGAAATTGTTTT+TGG + chr7_4:30618767-30618786 Msa1149360:CDS 25.0%
!!! GAAATTGTTTTTGGTTACTA+CGG + chr7_4:30618776-30618795 Msa1149360:CDS 25.0%
! AATGTTAAATGAAGAAGGAA+TGG - chr7_4:30618980-30618999 None:intergenic 25.0%
! GGATAAATGTTAAATGAAGA+AGG - chr7_4:30618985-30619004 None:intergenic 25.0%
! TCATTTAACATTTATCCGAT+TGG + chr7_4:30618988-30619007 Msa1149360:intron 25.0%
! CTGTATAAAACAATACCAAT+CGG - chr7_4:30619006-30619025 None:intergenic 25.0%
!! ATAAAACTTTGTGCTGATAA+TGG + chr7_4:30619077-30619096 Msa1149360:CDS 25.0%
! AAGGTGTATTTACTTAATGA+TGG + chr7_4:30619116-30619135 Msa1149360:CDS 25.0%
AAATTTGTCTTCACAAAAGC+TGG - chr7_4:30618614-30618633 None:intergenic 30.0%
CTACGGTAATAATATGTTCT+CGG + chr7_4:30618793-30618812 Msa1149360:CDS 30.0%
TGGCAATGAAGAGAAATTTA+AGG + chr7_4:30619097-30619116 Msa1149360:CDS 30.0%
GGAAAACTTACAACACAATT+TGG + chr7_4:30619137-30619156 Msa1149360:CDS 30.0%
! TCAATTTGAAGCGTATTGTT+TGG - chr7_4:30619208-30619227 None:intergenic 30.0%
AAATTTGTCTTCACAAAAGC+TGG - chr7_4:30618614-30618633 None:intergenic 30.0%
CTACGGTAATAATATGTTCT+CGG + chr7_4:30618793-30618812 Msa1149360:CDS 30.0%
TGGCAATGAAGAGAAATTTA+AGG + chr7_4:30619097-30619116 Msa1149360:CDS 30.0%
GGAAAACTTACAACACAATT+TGG + chr7_4:30619137-30619156 Msa1149360:CDS 30.0%
! TCAATTTGAAGCGTATTGTT+TGG - chr7_4:30619208-30619227 None:intergenic 30.0%
ACAACTGAGACATATTCAAG+CGG - chr7_4:30618447-30618466 None:intergenic 35.0%
TAGCTAATCTATCACGTTCA+TGG + chr7_4:30618633-30618652 Msa1149360:CDS 35.0%
CTTCTCAATACAGAAAACAC+AGG + chr7_4:30618659-30618678 Msa1149360:CDS 35.0%
ATCAAAATACGCAGTGTGAT+TGG - chr7_4:30618885-30618904 None:intergenic 35.0%
!! TTTTGATTCTCTCCTTACTG+AGG + chr7_4:30618898-30618917 Msa1149360:intron 35.0%
GGAATGTATCTCAAGCAATA+TGG + chr7_4:30619044-30619063 Msa1149360:CDS 35.0%
!! CAGCACAAAGTTTTATATCC+TGG - chr7_4:30619073-30619092 None:intergenic 35.0%
! AACACAATTTGGAATCCTGT+GGG + chr7_4:30619148-30619167 Msa1149360:CDS 35.0%
ACAACTGAGACATATTCAAG+CGG - chr7_4:30618447-30618466 None:intergenic 35.0%
TAGCTAATCTATCACGTTCA+TGG + chr7_4:30618633-30618652 Msa1149360:CDS 35.0%
CTTCTCAATACAGAAAACAC+AGG + chr7_4:30618659-30618678 Msa1149360:CDS 35.0%
ATCAAAATACGCAGTGTGAT+TGG - chr7_4:30618885-30618904 None:intergenic 35.0%
!! TTTTGATTCTCTCCTTACTG+AGG + chr7_4:30618898-30618917 Msa1149360:intron 35.0%
GGAATGTATCTCAAGCAATA+TGG + chr7_4:30619044-30619063 Msa1149360:CDS 35.0%
!! CAGCACAAAGTTTTATATCC+TGG - chr7_4:30619073-30619092 None:intergenic 35.0%
! AACACAATTTGGAATCCTGT+GGG + chr7_4:30619148-30619167 Msa1149360:CDS 35.0%
CAACTGAGACATATTCAAGC+GGG - chr7_4:30618446-30618465 None:intergenic 40.0%
GTTAACCCCATGTTAACACA+TGG + chr7_4:30618710-30618729 Msa1149360:CDS 40.0%
TCCAACCATGTGTTAACATG+GGG - chr7_4:30618718-30618737 None:intergenic 40.0%
GTCCAACCATGTGTTAACAT+GGG - chr7_4:30618719-30618738 None:intergenic 40.0%
AGTCCAACCATGTGTTAACA+TGG - chr7_4:30618720-30618739 None:intergenic 40.0%
ACATGGTTGGACTGAGTTTA+AGG + chr7_4:30618727-30618746 Msa1149360:CDS 40.0%
CACCTCATGACTTACATACA+TGG + chr7_4:30618840-30618859 Msa1149360:intron 40.0%
TGCCATGTATGTAAGTCATG+AGG - chr7_4:30618845-30618864 None:intergenic 40.0%
TGAGAAAGTCATCCTCAGTA+AGG - chr7_4:30618913-30618932 None:intergenic 40.0%
!! CAGCAAGTTGATGACGAATT+TGG + chr7_4:30619023-30619042 Msa1149360:intron 40.0%
TCTCAAGCAATATGGTTTCC+AGG + chr7_4:30619052-30619071 Msa1149360:CDS 40.0%
! CAACACAATTTGGAATCCTG+TGG + chr7_4:30619147-30619166 Msa1149360:CDS 40.0%
AAGACCAACAACTTCAGAGT+TGG + chr7_4:30619182-30619201 Msa1149360:CDS 40.0%
CAACTGAGACATATTCAAGC+GGG - chr7_4:30618446-30618465 None:intergenic 40.0%
GTTAACCCCATGTTAACACA+TGG + chr7_4:30618710-30618729 Msa1149360:CDS 40.0%
TCCAACCATGTGTTAACATG+GGG - chr7_4:30618718-30618737 None:intergenic 40.0%
GTCCAACCATGTGTTAACAT+GGG - chr7_4:30618719-30618738 None:intergenic 40.0%
AGTCCAACCATGTGTTAACA+TGG - chr7_4:30618720-30618739 None:intergenic 40.0%
ACATGGTTGGACTGAGTTTA+AGG + chr7_4:30618727-30618746 Msa1149360:CDS 40.0%
CACCTCATGACTTACATACA+TGG + chr7_4:30618840-30618859 Msa1149360:intron 40.0%
TGCCATGTATGTAAGTCATG+AGG - chr7_4:30618845-30618864 None:intergenic 40.0%
TGAGAAAGTCATCCTCAGTA+AGG - chr7_4:30618913-30618932 None:intergenic 40.0%
!! CAGCAAGTTGATGACGAATT+TGG + chr7_4:30619023-30619042 Msa1149360:intron 40.0%
TCTCAAGCAATATGGTTTCC+AGG + chr7_4:30619052-30619071 Msa1149360:CDS 40.0%
! CAACACAATTTGGAATCCTG+TGG + chr7_4:30619147-30619166 Msa1149360:CDS 40.0%
AAGACCAACAACTTCAGAGT+TGG + chr7_4:30619182-30619201 Msa1149360:CDS 40.0%
AGTTGTCAAAGTTGCAGAGC+AGG + chr7_4:30618461-30618480 Msa1149360:CDS 45.0%
ACCCCATGTTAACACATGGT+TGG + chr7_4:30618714-30618733 Msa1149360:CDS 45.0%
TCTTGCAGAAATGATCCCAC+AGG - chr7_4:30619166-30619185 None:intergenic 45.0%
!! TTGGCCAACTCTGAAGTTGT+TGG - chr7_4:30619189-30619208 None:intergenic 45.0%
AGTTGTCAAAGTTGCAGAGC+AGG + chr7_4:30618461-30618480 Msa1149360:CDS 45.0%
ACCCCATGTTAACACATGGT+TGG + chr7_4:30618714-30618733 Msa1149360:CDS 45.0%
TCTTGCAGAAATGATCCCAC+AGG - chr7_4:30619166-30619185 None:intergenic 45.0%
!! TTGGCCAACTCTGAAGTTGT+TGG - chr7_4:30619189-30619208 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 30618411 30619235 30618411 ID=Msa1149360;Name=Msa1149360
chr7_4 mRNA 30618411 30619235 30618411 ID=Msa1149360-mRNA-1;Parent=Msa1149360;Name=Msa1149360-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|3|0|167
chr7_4 exon 30618411 30618482 30618411 ID=Msa1149360-mRNA-1:exon:7462;Parent=Msa1149360-mRNA-1
chr7_4 exon 30618593 30618814 30618593 ID=Msa1149360-mRNA-1:exon:7463;Parent=Msa1149360-mRNA-1
chr7_4 exon 30619026 30619235 30619026 ID=Msa1149360-mRNA-1:exon:7464;Parent=Msa1149360-mRNA-1
chr7_4 CDS 30618411 30618482 30618411 ID=Msa1149360-mRNA-1:cds;Parent=Msa1149360-mRNA-1
chr7_4 CDS 30618593 30618814 30618593 ID=Msa1149360-mRNA-1:cds;Parent=Msa1149360-mRNA-1
chr7_4 CDS 30619026 30619235 30619026 ID=Msa1149360-mRNA-1:cds;Parent=Msa1149360-mRNA-1
Gene Sequence

>Msa1149360

ATGGAAAAAATCAGATCTTTGCTTCAAAGAGACCCGCTTGAATATGTCTCAGTTGTCAAAGTTGCAGAGCAGATGCATCGTGAGATCGAACCAGCTTTTGTGAAGACAAATTTAGCTAATCTATCACGTTCATGGCATCTTCTCAATACAGAAAACACAGGTCACATTTTAACTTTCAACAAAAGCTTGGTTAACCCCATGTTAACACATGGTTGGACTGAGTTTAAGGATTTTAATGATCTTCCTGACAATGTTGAAATTGTTTTTGGTTACTACGGTAATAATATGTTCTCGCAAGTTGATGACGAATTTGGAATGTATCTCAAGCAATATGGTTTCCAGGATATAAAACTTTGTGCTGATAATGGCAATGAAGAGAAATTTAAGGTGTATTTACTTAATGATGGAAAACTTACAACACAATTTGGAATCCTGTGGGATCATTTCTGCAAGACCAACAACTTCAGAGTTGGCCAAACAATACGCTTCAAATTGATGCTTTAA

Protein sequence

>Msa1149360

MEKIRSLLQRDPLEYVSVVKVAEQMHREIEPAFVKTNLANLSRSWHLLNTENTGHILTFNKSLVNPMLTHGWTEFKDFNDLPDNVEIVFGYYGNNMFSQVDDEFGMYLKQYGFQDIKLCADNGNEEKFKVYLLNDGKLTTQFGILWDHFCKTNNFRVGQTIRFKLML*