AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1015490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1015490 MtrunA17_Chr2g0308161 66.129 62 21 0 1 62 7 68 7.37e-19 73.2
Msa1015490 MtrunA17_Chr3g0100521 70.000 50 12 1 13 62 51 97 8.53e-12 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAGGTGAATGTCAAATT+AGG 0.211924 7_1:+28092490 None:intergenic
AATCGCGTTCTTATAGGATT+TGG 0.249043 7_1:+28092572 None:intergenic
TGAATGTCAAATTAGGGATT+TGG 0.256068 7_1:+28092497 None:intergenic
CTTCTTCACAAGTTGTTAGA+TGG 0.286619 7_1:+28092151 None:intergenic
TCAAATTAGGGATTTGGGTT+AGG 0.300087 7_1:+28092503 None:intergenic
ATTGTAGTTAAACCACATTT+TGG 0.314764 7_1:-28089180 Msa1015490:three_prime_UTR
AGGAAGAATCGCGTTCTTAT+AGG 0.319015 7_1:+28092566 None:intergenic
TGAAGCTGAATCGTGAGATT+AGG 0.342464 7_1:+28092436 None:intergenic
ATCGCGTTCTTATAGGATTT+GGG 0.346236 7_1:+28092573 None:intergenic
TTCTTATAGGATTTGGGATC+AGG 0.346645 7_1:+28092579 None:intergenic
GAATGTCAAATTAGGGATTT+GGG 0.350675 7_1:+28092498 None:intergenic
GATCAAGACAATCTCTAATC+TGG 0.363464 7_1:-28092398 Msa1015490:five_prime_UTR
GAAGCTGAATCGTGAGATTA+GGG 0.368219 7_1:+28092437 None:intergenic
GGAAGGTGAATGTCAAATTA+GGG 0.368596 7_1:+28092491 None:intergenic
CAAATTAGGGATTTGGGTTA+GGG 0.368857 7_1:+28092504 None:intergenic
ATCTGACGAAGATGAAACTA+TGG 0.379407 7_1:+28092604 None:intergenic
AGAGATAACATTTGTAGATC+TGG 0.390855 7_1:+28092753 None:intergenic
TCCTCTTCTTCATTGACTAC+CGG 0.415639 7_1:+28090036 None:intergenic
TTTGAAGGATTTGATGAAGA+AGG 0.420761 7_1:-28092213 Msa1015490:five_prime_UTR
TATAGTTCGGACAAGTTTAG+TGG 0.422017 7_1:+28089145 None:intergenic
TAAGAAAGAGAAGGGCAAAT+TGG 0.437928 7_1:+28092842 None:intergenic
CAACTTAACTGATGAATTTG+AGG 0.446814 7_1:-28089270 Msa1015490:CDS
GCACTTCATATTATGAATCT+CGG 0.454592 7_1:-28090126 Msa1015490:five_prime_UTR
CACTTCATATTATGAATCTC+GGG 0.455058 7_1:-28090125 Msa1015490:five_prime_UTR
TTTGTAGATCTGGACATTAG+AGG 0.457190 7_1:+28092763 None:intergenic
GATGGAACCCATCGAGTTCT+CGG 0.462992 7_1:+28092169 None:intergenic
CATCTAACAACTTGTGAAGA+AGG 0.463766 7_1:-28092150 Msa1015490:five_prime_UTR
AGAAGGGTATGCTTTATAGT+AGG 0.464947 7_1:-28092196 Msa1015490:five_prime_UTR
CGAAGATGAAACTATGGATT+GGG 0.477095 7_1:+28092610 None:intergenic
TTTCAACTTCCAATCGCAAT+GGG 0.482215 7_1:-28092373 Msa1015490:five_prime_UTR
ACAGAAGAACCCATTGCGAT+TGG 0.482264 7_1:+28092364 None:intergenic
TTGAAGGATTTGATGAAGAA+GGG 0.482747 7_1:-28092212 Msa1015490:five_prime_UTR
CTCATTCTACTTCGATTGGA+AGG 0.483936 7_1:+28092474 None:intergenic
TGGGACTCTTGAATTGAAGC+AGG 0.485151 7_1:+28092629 None:intergenic
AGTGGAAGATTCGAGAGAAC+CGG 0.486411 7_1:-28090055 Msa1015490:five_prime_UTR
TGTTTGACACTTTGATTGCT+AGG 0.491693 7_1:-28089070 Msa1015490:three_prime_UTR
TGTTCTCATTCTACTTCGAT+TGG 0.493266 7_1:+28092470 None:intergenic
AAAATAACAAAACTATAGTT+CGG 0.493711 7_1:+28089132 None:intergenic
ACGAAGATGAAACTATGGAT+TGG 0.499837 7_1:+28092609 None:intergenic
TATCAATGAAGATGTTGTAC+AGG 0.507268 7_1:-28089363 Msa1015490:CDS
ATTTCAACTTCCAATCGCAA+TGG 0.550801 7_1:-28092374 Msa1015490:five_prime_UTR
ACAAAGATGTAAGAAAGAGA+AGG 0.556908 7_1:+28092833 None:intergenic
ACCGGTAGTCAATGAAGAAG+AGG 0.561584 7_1:-28090037 Msa1015490:exon
CAAAGATGTAAGAAAGAGAA+GGG 0.564798 7_1:+28092834 None:intergenic
TTTAACTACAATCCAAAATG+TGG 0.569276 7_1:+28089168 None:intergenic
ATCTAACAACTTGTGAAGAA+GGG 0.574304 7_1:-28092149 Msa1015490:intron
ATAAACTTGAATGATCGAGT+TGG 0.577612 7_1:-28089220 Msa1015490:exon
AGATTGAATCAGCGAAAATG+AGG 0.579872 7_1:+28092673 None:intergenic
TTTGGTATGTGGGAGTCTTG+TGG 0.580729 7_1:-28090153 Msa1015490:five_prime_UTR
TGTACAGGATGACATAGATA+AGG 0.601176 7_1:-28089348 Msa1015490:CDS
TAGGATTACCGAGAACTCGA+TGG 0.605153 7_1:-28092177 Msa1015490:five_prime_UTR
AGGATTACCGAGAACTCGAT+GGG 0.605678 7_1:-28092176 Msa1015490:five_prime_UTR
GAGGATAGAGAAGACGCAAG+TGG 0.617949 7_1:+28092782 None:intergenic
GAATTGAAGCAGGGAACAAG+AGG 0.620677 7_1:+28092639 None:intergenic
GGGACTCTTGAATTGAAGCA+GGG 0.623227 7_1:+28092630 None:intergenic
CATAGATAAGGAATTTCAAG+AGG 0.629359 7_1:-28089336 Msa1015490:CDS
CTTAACTGATGAATTTGAGG+AGG 0.634997 7_1:-28089267 Msa1015490:CDS
ACTTGAATGATCGAGTTGGA+TGG 0.679049 7_1:-28089216 Msa1015490:exon
TCAGCCACTGTCTAGCAGAG+TGG 0.692608 7_1:-28090073 Msa1015490:five_prime_UTR
TCTTCCACTCTGCTAGACAG+TGG 0.709415 7_1:+28090069 None:intergenic
ACAAATAGAGAAGAAGAACA+AGG 0.723653 7_1:-28090009 Msa1015490:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTATTTTTATTTTT+TGG - chr7_1:28089615-28089634 Msa1015490:intron 0.0%
!!! TATTTTTTGGTATTTTTTTT+AGG - chr7_1:28089628-28089647 Msa1015490:intron 10.0%
!! ATTTATCTTCATTTATTTAT+TGG - chr7_1:28089762-28089781 Msa1015490:intron 10.0%
!! TAAGTTAATTTATTTAAAGA+TGG + chr7_1:28091148-28091167 None:intergenic 10.0%
!!! TTTTTAGATAAATAAATAAG+AGG - chr7_1:28091667-28091686 Msa1015490:intron 10.0%
!!! TTTTAGATAAATAAATAAGA+GGG - chr7_1:28091668-28091687 Msa1015490:intron 10.0%
!!! TATTTTTTTTAGGATTTTGA+AGG - chr7_1:28089638-28089657 Msa1015490:intron 15.0%
!! ATTTCGTATTTATGTTTATA+TGG - chr7_1:28090481-28090500 Msa1015490:intron 15.0%
!!! ATTTCTTGTTATACATATTT+AGG - chr7_1:28091421-28091440 Msa1015490:intron 15.0%
!!! TTTCTTGTTATACATATTTA+GGG - chr7_1:28091422-28091441 Msa1015490:intron 15.0%
!! TTTATCTAAAAACTAAAACA+TGG + chr7_1:28091659-28091678 None:intergenic 15.0%
!! TTCATGTATTCTTTATATAT+TGG - chr7_1:28091909-28091928 Msa1015490:intron 15.0%
!! AAAATAACAAAACTATAGTT+CGG + chr7_1:28092737-28092756 None:intergenic 15.0%
!! AAATCTAATTCATAGTTCTT+TGG + chr7_1:28090131-28090150 None:intergenic 20.0%
!! AACATAAATACGAAATGAAT+GGG + chr7_1:28090477-28090496 None:intergenic 20.0%
!! AAACATAAATACGAAATGAA+TGG + chr7_1:28090478-28090497 None:intergenic 20.0%
!! ATAGGAAAAACTTAAACAAT+AGG + chr7_1:28090520-28090539 None:intergenic 20.0%
!! TTTCCTATAAAATTTGTAAC+TGG - chr7_1:28090532-28090551 Msa1015490:intron 20.0%
!!! TAACCAGTTACAAATTTTAT+AGG + chr7_1:28090538-28090557 None:intergenic 20.0%
!! TATAAAATTTGTAACTGGTT+AGG - chr7_1:28090537-28090556 Msa1015490:intron 20.0%
!!! AATCAATTTGTTATTAGACA+TGG + chr7_1:28090589-28090608 None:intergenic 20.0%
!!! TCATCAAAAATTGTTTTTCT+TGG - chr7_1:28090680-28090699 Msa1015490:intron 20.0%
!! TTTAACAAATGTTGATCAAT+TGG - chr7_1:28090973-28090992 Msa1015490:intron 20.0%
!! TTTGCACATTTAAACATAAT+TGG + chr7_1:28091362-28091381 None:intergenic 20.0%
!! AATTATGTTTAAATGTGCAA+AGG - chr7_1:28091361-28091380 Msa1015490:intron 20.0%
!!! TTCTTGTTATACATATTTAG+GGG - chr7_1:28091423-28091442 Msa1015490:intron 20.0%
!!! AACACCAAAAAGTATAAAAA+AGG + chr7_1:28091454-28091473 None:intergenic 20.0%
!!! ATCAACATGTGAAATTTTTT+TGG - chr7_1:28091934-28091953 Msa1015490:intron 20.0%
!!! AATTTTGTTTATGCAATTTG+TGG - chr7_1:28092203-28092222 Msa1015490:five_prime_UTR 20.0%
!!! TATAGTTTTGTTATTTTGCT+AGG - chr7_1:28092740-28092759 Msa1015490:five_prime_UTR 20.0%
!!! TTTTGGTGGATTTTGTATTT+TGG + chr7_1:28089149-28089168 None:intergenic 25.0%
!! TCACAAAGAAGATGAATTTT+TGG + chr7_1:28089166-28089185 None:intergenic 25.0%
!!! ATTCTTCCTTTTTTTCAACA+AGG - chr7_1:28089314-28089333 Msa1015490:CDS 25.0%
! CTTAAACCTTGTTGAAAAAA+AGG + chr7_1:28089323-28089342 None:intergenic 25.0%
!!! TTTCTTGTTCTTGTTGATTT+TGG - chr7_1:28089583-28089602 Msa1015490:intron 25.0%
!!! TTCTTGTTCTTGTTGATTTT+GGG - chr7_1:28089584-28089603 Msa1015490:intron 25.0%
!!! TTGAATTTTGAAGAGACTTT+TGG - chr7_1:28089856-28089875 Msa1015490:intron 25.0%
! TAGACATTGTATAGCATAAT+TGG - chr7_1:28089926-28089945 Msa1015490:intron 25.0%
! TAATTGGAAGAAATTTGTAC+AGG - chr7_1:28089942-28089961 Msa1015490:intron 25.0%
! CAAGTAATGTAATGTAGTAA+TGG + chr7_1:28090281-28090300 None:intergenic 25.0%
! ACATAAATACGAAATGAATG+GGG + chr7_1:28090476-28090495 None:intergenic 25.0%
! AAAAACTTAAACAATAGGTG+AGG + chr7_1:28090515-28090534 None:intergenic 25.0%
! TATAAGTGTTATATAGGTGT+CGG + chr7_1:28090864-28090883 None:intergenic 25.0%
! GATCGTAGATGAATAAATAA+AGG - chr7_1:28091029-28091048 Msa1015490:intron 25.0%
! ATAAATTAACTTACATGCAG+CGG - chr7_1:28091155-28091174 Msa1015490:intron 25.0%
! ATTATATAACCTATGAAGCA+TGG + chr7_1:28091236-28091255 None:intergenic 25.0%
!!! GGATCCTTTTTTATACTTTT+TGG - chr7_1:28091447-28091466 Msa1015490:intron 25.0%
!!! CGAATTTATTTTTCATCACT+TGG - chr7_1:28092042-28092061 Msa1015490:intron 25.0%
!! AAACAATCAAAATCCGATTT+TGG + chr7_1:28092166-28092185 None:intergenic 25.0%
! TTGTAATGATTGAATAAGGA+AGG - chr7_1:28092319-28092338 Msa1015490:intron 25.0%
! TGTAATGATTGAATAAGGAA+GGG - chr7_1:28092320-28092339 Msa1015490:intron 25.0%
!!! CGTATATGATGAATTTTTTG+AGG - chr7_1:28092557-28092576 Msa1015490:five_prime_UTR 25.0%
!! ATTGTAGTTAAACCACATTT+TGG - chr7_1:28092686-28092705 Msa1015490:five_prime_UTR 25.0%
! TTTAACTACAATCCAAAATG+TGG + chr7_1:28092701-28092720 None:intergenic 25.0%
CAAAGATGTAAGAAAGAGAA+GGG + chr7_1:28089035-28089054 None:intergenic 30.0%
ACAAAGATGTAAGAAAGAGA+AGG + chr7_1:28089036-28089055 None:intergenic 30.0%
AGAGATAACATTTGTAGATC+TGG + chr7_1:28089116-28089135 None:intergenic 30.0%
!! CAAAGAAGATGAATTTTTGG+TGG + chr7_1:28089163-28089182 None:intergenic 30.0%
GAATGTCAAATTAGGGATTT+GGG + chr7_1:28089371-28089390 None:intergenic 30.0%
TGAATGTCAAATTAGGGATT+TGG + chr7_1:28089372-28089391 None:intergenic 30.0%
!! TGTCTTGATCTCATTTTTCT+AGG + chr7_1:28089458-28089477 None:intergenic 30.0%
!!! TCTTGTTCTTGTTGATTTTG+GGG - chr7_1:28089585-28089604 Msa1015490:intron 30.0%
TTTGAAGGATTTGATGAAGA+AGG - chr7_1:28089653-28089672 Msa1015490:intron 30.0%
TTGAAGGATTTGATGAAGAA+GGG - chr7_1:28089654-28089673 Msa1015490:intron 30.0%
ATCTAACAACTTGTGAAGAA+GGG - chr7_1:28089717-28089736 Msa1015490:intron 30.0%
!!! TTATTGGTTTTGATTGCCTA+AGG - chr7_1:28089778-28089797 Msa1015490:intron 30.0%
! TGTGTTGCTATGTTTGTATT+TGG - chr7_1:28089802-28089821 Msa1015490:intron 30.0%
! TTTTGGACTTATCCTAATCA+TGG - chr7_1:28089873-28089892 Msa1015490:intron 30.0%
!! TTATCCTAATCATGGTTTTG+TGG - chr7_1:28089881-28089900 Msa1015490:intron 30.0%
! TGTGGCTAGCAAAATTTTAT+AGG - chr7_1:28089899-28089918 Msa1015490:intron 30.0%
TTCATTAAGTACCAAATACC+AGG + chr7_1:28090033-28090052 None:intergenic 30.0%
TAATGTATTGAATGTCTTCC+TGG + chr7_1:28090073-28090092 None:intergenic 30.0%
GTCTAATATTTCCATAGTGA+AGG - chr7_1:28090191-28090210 Msa1015490:intron 30.0%
TTTATATATCGCCTTCACTA+TGG + chr7_1:28090205-28090224 None:intergenic 30.0%
ATAGTGAAGGCGATATATAA+AGG - chr7_1:28090204-28090223 Msa1015490:intron 30.0%
CATAAATACGAAATGAATGG+GGG + chr7_1:28090475-28090494 None:intergenic 30.0%
! TTGTTATTAGACATGGAGAA+GGG + chr7_1:28090582-28090601 None:intergenic 30.0%
!! TTTGTTATTAGACATGGAGA+AGG + chr7_1:28090583-28090602 None:intergenic 30.0%
!!! TTTTAAAGTAGTACACACTG+AGG + chr7_1:28090656-28090675 None:intergenic 30.0%
GGCTAGTATAAGTGTTATAT+AGG + chr7_1:28090870-28090889 None:intergenic 30.0%
TTGTTATACATATTTAGGGG+TGG - chr7_1:28091426-28091445 Msa1015490:intron 30.0%
ATATTGTTGTGACTATCTCA+TGG - chr7_1:28091581-28091600 Msa1015490:intron 30.0%
!!! TAGGGTTTTTTTTTGGTATG+TGG - chr7_1:28091702-28091721 Msa1015490:intron 30.0%
!!! AGGGTTTTTTTTTGGTATGT+GGG - chr7_1:28091703-28091722 Msa1015490:intron 30.0%
GCACTTCATATTATGAATCT+CGG - chr7_1:28091740-28091759 Msa1015490:intron 30.0%
CACTTCATATTATGAATCTC+GGG - chr7_1:28091741-28091760 Msa1015490:intron 30.0%
ACAAATAGAGAAGAAGAACA+AGG - chr7_1:28091857-28091876 Msa1015490:intron 30.0%
GAAAAATAAATTCGCACACA+AGG + chr7_1:28092036-28092055 None:intergenic 30.0%
! TTTTTCATCACTTGGAATGT+AGG - chr7_1:28092050-28092069 Msa1015490:intron 30.0%
CGAACTTATGATTATGTGTT+TGG - chr7_1:28092118-28092137 Msa1015490:intron 30.0%
GAGCTTGTAATGATTGAATA+AGG - chr7_1:28092315-28092334 Msa1015490:intron 30.0%
! TGACTGTGTTTTACTTGTTT+TGG - chr7_1:28092379-28092398 Msa1015490:five_prime_UTR 30.0%
!!! TTTTGGTTTTTATTTGTGCG+AGG - chr7_1:28092396-28092415 Msa1015490:five_prime_UTR 30.0%
!! CATAGATATTGTTGGTTCAA+AGG + chr7_1:28092450-28092469 None:intergenic 30.0%
CTTTGAACCAACAATATCTA+TGG - chr7_1:28092448-28092467 Msa1015490:five_prime_UTR 30.0%
ACATCAACCATAGATATTGT+TGG + chr7_1:28092458-28092477 None:intergenic 30.0%
TATCAATGAAGATGTTGTAC+AGG - chr7_1:28092503-28092522 Msa1015490:five_prime_UTR 30.0%
CATAGATAAGGAATTTCAAG+AGG - chr7_1:28092530-28092549 Msa1015490:five_prime_UTR 30.0%
CAACTTAACTGATGAATTTG+AGG - chr7_1:28092596-28092615 Msa1015490:five_prime_UTR 30.0%
ATAAACTTGAATGATCGAGT+TGG - chr7_1:28092646-28092665 Msa1015490:five_prime_UTR 30.0%
!!! AGTTGGATGGTGTTTTATTT+TGG - chr7_1:28092663-28092682 Msa1015490:five_prime_UTR 30.0%
TAAGAAAGAGAAGGGCAAAT+TGG + chr7_1:28089027-28089046 None:intergenic 35.0%
TTTGTAGATCTGGACATTAG+AGG + chr7_1:28089106-28089125 None:intergenic 35.0%
!!! TGGTGGATTTTGTATTTTGG+TGG + chr7_1:28089146-28089165 None:intergenic 35.0%
AGATTGAATCAGCGAAAATG+AGG + chr7_1:28089196-28089215 None:intergenic 35.0%
CGAAGATGAAACTATGGATT+GGG + chr7_1:28089259-28089278 None:intergenic 35.0%
ACGAAGATGAAACTATGGAT+TGG + chr7_1:28089260-28089279 None:intergenic 35.0%
ATCTGACGAAGATGAAACTA+TGG + chr7_1:28089265-28089284 None:intergenic 35.0%
! TTCTTATAGGATTTGGGATC+AGG + chr7_1:28089290-28089309 None:intergenic 35.0%
ATCGCGTTCTTATAGGATTT+GGG + chr7_1:28089296-28089315 None:intergenic 35.0%
AATCGCGTTCTTATAGGATT+TGG + chr7_1:28089297-28089316 None:intergenic 35.0%
! CAAATTAGGGATTTGGGTTA+GGG + chr7_1:28089365-28089384 None:intergenic 35.0%
! TCAAATTAGGGATTTGGGTT+AGG + chr7_1:28089366-28089385 None:intergenic 35.0%
GGAAGGTGAATGTCAAATTA+GGG + chr7_1:28089378-28089397 None:intergenic 35.0%
TGGAAGGTGAATGTCAAATT+AGG + chr7_1:28089379-28089398 None:intergenic 35.0%
TGTTCTCATTCTACTTCGAT+TGG + chr7_1:28089399-28089418 None:intergenic 35.0%
GATCAAGACAATCTCTAATC+TGG - chr7_1:28089468-28089487 Msa1015490:intron 35.0%
ATTTCAACTTCCAATCGCAA+TGG - chr7_1:28089492-28089511 Msa1015490:intron 35.0%
TTTCAACTTCCAATCGCAAT+GGG - chr7_1:28089493-28089512 Msa1015490:intron 35.0%
! AGAAGGGTATGCTTTATAGT+AGG - chr7_1:28089670-28089689 Msa1015490:intron 35.0%
CTTCTTCACAAGTTGTTAGA+TGG + chr7_1:28089718-28089737 None:intergenic 35.0%
CATCTAACAACTTGTGAAGA+AGG - chr7_1:28089716-28089735 Msa1015490:intron 35.0%
AAACATAGCAACACAACCTT+AGG + chr7_1:28089797-28089816 None:intergenic 35.0%
AAGACCACTATATATGCAAC+TGG - chr7_1:28090355-28090374 Msa1015490:intron 35.0%
CAAATTGATTAGCATGTGCA+TGG - chr7_1:28090599-28090618 Msa1015490:intron 35.0%
TTAGCATGTGCATGGTATAT+AGG - chr7_1:28090607-28090626 Msa1015490:intron 35.0%
TTATCATTCGTTTCATGCCA+TGG - chr7_1:28091273-28091292 Msa1015490:intron 35.0%
TATCATTCGTTTCATGCCAT+GGG - chr7_1:28091274-28091293 Msa1015490:intron 35.0%
!!! CTTTTTGGTGTTGTGTTGTT+TGG - chr7_1:28091462-28091481 Msa1015490:intron 35.0%
!!! GTGTTGTTTGGTTTTGTCAA+TGG - chr7_1:28091474-28091493 Msa1015490:intron 35.0%
AAATCATGTGTTGTAGTGTG+AGG - chr7_1:28091512-28091531 Msa1015490:intron 35.0%
!!! GCACTTGTAGGGTTTTTTTT+TGG - chr7_1:28091695-28091714 Msa1015490:intron 35.0%
!! GCTGATTTTTACTAGAGCTA+GGG + chr7_1:28091778-28091797 None:intergenic 35.0%
TTAAGATGCGTGTCCAAAAT+CGG - chr7_1:28092150-28092169 Msa1015490:five_prime_UTR 35.0%
TGTACAGGATGACATAGATA+AGG - chr7_1:28092518-28092537 Msa1015490:five_prime_UTR 35.0%
!! TTTTGAGGACGAATTTCATG+AGG - chr7_1:28092572-28092591 Msa1015490:five_prime_UTR 35.0%
CTTAACTGATGAATTTGAGG+AGG - chr7_1:28092599-28092618 Msa1015490:five_prime_UTR 35.0%
TATAGTTCGGACAAGTTTAG+TGG + chr7_1:28092724-28092743 None:intergenic 35.0%
TGTTTGACACTTTGATTGCT+AGG - chr7_1:28092796-28092815 Msa1015490:five_prime_UTR 35.0%
AGGAAGAATCGCGTTCTTAT+AGG + chr7_1:28089303-28089322 None:intergenic 40.0%
CTCATTCTACTTCGATTGGA+AGG + chr7_1:28089395-28089414 None:intergenic 40.0%
GAAGCTGAATCGTGAGATTA+GGG + chr7_1:28089432-28089451 None:intergenic 40.0%
TGAAGCTGAATCGTGAGATT+AGG + chr7_1:28089433-28089452 None:intergenic 40.0%
CTAGCCACAAAACCATGATT+AGG + chr7_1:28089888-28089907 None:intergenic 40.0%
GTGATCGTGTGTGTTGAATT+AGG - chr7_1:28090007-28090026 Msa1015490:intron 40.0%
GTTGAATTAGGCCTGGTATT+TGG - chr7_1:28090019-28090038 Msa1015490:CDS 40.0%
!! AATAGAGAGCTAAAAGCACC+AGG - chr7_1:28090052-28090071 Msa1015490:five_prime_UTR 40.0%
! ATAGTGGTCTTGAGTGCTTA+CGG + chr7_1:28090346-28090365 None:intergenic 40.0%
GACTCCAGTTGCATATATAG+TGG + chr7_1:28090362-28090381 None:intergenic 40.0%
ATGGAGGTGGAGTATACAAT+TGG + chr7_1:28090445-28090464 None:intergenic 40.0%
TGTATACTCCACCTCCATAA+TGG - chr7_1:28090447-28090466 Msa1015490:intron 40.0%
AATGAATGGGGGTTCCATTA+TGG + chr7_1:28090464-28090483 None:intergenic 40.0%
!! CTATGAAGCATTGACACACT+AGG - chr7_1:28090711-28090730 Msa1015490:intron 40.0%
TTTGTCTGAGTTGTCTGACA+CGG + chr7_1:28090891-28090910 None:intergenic 40.0%
ACTCTTGTCTCTATAGTGTG+TGG + chr7_1:28090944-28090963 None:intergenic 40.0%
ATCATTCGTTTCATGCCATG+GGG - chr7_1:28091275-28091294 Msa1015490:intron 40.0%
TAAGAGGGTTTAGCACTTGT+AGG - chr7_1:28091683-28091702 Msa1015490:intron 40.0%
AAGAGGGTTTAGCACTTGTA+GGG - chr7_1:28091684-28091703 Msa1015490:intron 40.0%
!! GGCTGATTTTTACTAGAGCT+AGG + chr7_1:28091779-28091798 None:intergenic 40.0%
! TCCTCTTCTTCATTGACTAC+CGG + chr7_1:28091833-28091852 None:intergenic 40.0%
ACACGCAACTTAACAGTTTC+AGG + chr7_1:28091966-28091985 None:intergenic 40.0%
! AACTGTTAAGTTGCGTGTGT+AGG - chr7_1:28091968-28091987 Msa1015490:intron 40.0%
! ACTGTTAAGTTGCGTGTGTA+GGG - chr7_1:28091969-28091988 Msa1015490:intron 40.0%
!! ACTTGAATGATCGAGTTGGA+TGG - chr7_1:28092650-28092669 Msa1015490:five_prime_UTR 40.0%
GAATTGAAGCAGGGAACAAG+AGG + chr7_1:28089230-28089249 None:intergenic 45.0%
GGGACTCTTGAATTGAAGCA+GGG + chr7_1:28089239-28089258 None:intergenic 45.0%
TGGGACTCTTGAATTGAAGC+AGG + chr7_1:28089240-28089259 None:intergenic 45.0%
ACAGAAGAACCCATTGCGAT+TGG + chr7_1:28089505-28089524 None:intergenic 45.0%
TAGGATTACCGAGAACTCGA+TGG - chr7_1:28089689-28089708 Msa1015490:intron 45.0%
AGGATTACCGAGAACTCGAT+GGG - chr7_1:28089690-28089709 Msa1015490:intron 45.0%
AGACTTTGTGTGCAAGACAG+TGG - chr7_1:28090306-28090325 Msa1015490:intron 45.0%
!! AGCATTGACACACTAGGACT+AGG - chr7_1:28090717-28090736 Msa1015490:intron 45.0%
! TACGAGTGTCATAGGAGTGT+CGG + chr7_1:28090773-28090792 None:intergenic 45.0%
TACTTCGACACTCCTACGAT+CGG - chr7_1:28090835-28090854 Msa1015490:intron 45.0%
!! ATGCAGCGGTGTTACATTAG+CGG - chr7_1:28091169-28091188 Msa1015490:intron 45.0%
CTAACATTAACCCAACCCCA+TGG + chr7_1:28091293-28091312 None:intergenic 45.0%
TTTGGTATGTGGGAGTCTTG+TGG - chr7_1:28091713-28091732 Msa1015490:intron 45.0%
AGTGGAAGATTCGAGAGAAC+CGG - chr7_1:28091811-28091830 Msa1015490:intron 45.0%
ACCGGTAGTCAATGAAGAAG+AGG - chr7_1:28091829-28091848 Msa1015490:intron 45.0%
GAGGATAGAGAAGACGCAAG+TGG + chr7_1:28089087-28089106 None:intergenic 50.0%
GATGGAACCCATCGAGTTCT+CGG + chr7_1:28089700-28089719 None:intergenic 50.0%
CGTGTGTGTTGAATTAGGCC+TGG - chr7_1:28090012-28090031 Msa1015490:CDS 50.0%
GAATGGGGGTTCCATTATGG+AGG + chr7_1:28090461-28090480 None:intergenic 50.0%
CATGGAGAAGGGATCACACT+TGG + chr7_1:28090571-28090590 None:intergenic 50.0%
ACGTGTCGTACGAGTGTCAT+AGG + chr7_1:28090781-28090800 None:intergenic 50.0%
GTCATGTCAAGTGTCCGTGT+CGG - chr7_1:28091207-28091226 Msa1015490:intron 50.0%
ATGAAGCATGGACTCCGACA+CGG + chr7_1:28091224-28091243 None:intergenic 50.0%
TGTCGGAGTCCATGCTTCAT+AGG - chr7_1:28091224-28091243 Msa1015490:intron 50.0%
TTCGTTTCATGCCATGGGGT+TGG - chr7_1:28091279-28091298 Msa1015490:intron 50.0%
TCGTTTCATGCCATGGGGTT+GGG - chr7_1:28091280-28091299 Msa1015490:intron 50.0%
TCTTCCACTCTGCTAGACAG+TGG + chr7_1:28091800-28091819 None:intergenic 50.0%
TGGGGGTTCCATTATGGAGG+TGG + chr7_1:28090458-28090477 None:intergenic 55.0%
CACTAGGACTAGGCGTGTCA+CGG - chr7_1:28090727-28090746 Msa1015490:intron 55.0%
TCGTACGACACGTGTCGAAC+TGG - chr7_1:28090788-28090807 Msa1015490:intron 55.0%
TCAGCCACTGTCTAGCAGAG+TGG - chr7_1:28091793-28091812 Msa1015490:intron 55.0%
!! AGGTGTCGGACACCGATCGT+AGG + chr7_1:28090850-28090869 None:intergenic 60.0%
TCACGGTGTCCGACACGCGT+CGG - chr7_1:28090744-28090763 Msa1015490:intron 65.0%
TGTCGGACACCGACGCGTGT+CGG + chr7_1:28090756-28090775 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 28089017 28092871 28089017 ID=Msa1015490;Name=Msa1015490
chr7_1 mRNA 28089017 28092871 28089017 ID=Msa1015490-mRNA-1;Parent=Msa1015490;Name=Msa1015490-mRNA-1;_AED=0.39;_eAED=0.39;_QI=741|1|1|1|0.33|0.25|4|212|66
chr7_1 exon 28089017 28089390 28089017 ID=Msa1015490-mRNA-1:exon:6011;Parent=Msa1015490-mRNA-1
chr7_1 exon 28090010 28090183 28090010 ID=Msa1015490-mRNA-1:exon:6010;Parent=Msa1015490-mRNA-1
chr7_1 exon 28092150 28092238 28092150 ID=Msa1015490-mRNA-1:exon:6009;Parent=Msa1015490-mRNA-1
chr7_1 exon 28092355 28092871 28092355 ID=Msa1015490-mRNA-1:exon:6008;Parent=Msa1015490-mRNA-1
chr7_1 five_prime_UTR 28092355 28092871 28092355 ID=Msa1015490-mRNA-1:five_prime_utr;Parent=Msa1015490-mRNA-1
chr7_1 five_prime_UTR 28092150 28092238 28092150 ID=Msa1015490-mRNA-1:five_prime_utr;Parent=Msa1015490-mRNA-1
chr7_1 five_prime_UTR 28090049 28090183 28090049 ID=Msa1015490-mRNA-1:five_prime_utr;Parent=Msa1015490-mRNA-1
chr7_1 CDS 28090010 28090048 28090010 ID=Msa1015490-mRNA-1:cds;Parent=Msa1015490-mRNA-1
chr7_1 CDS 28089229 28089390 28089229 ID=Msa1015490-mRNA-1:cds;Parent=Msa1015490-mRNA-1
chr7_1 three_prime_UTR 28089017 28089228 28089017 ID=Msa1015490-mRNA-1:three_prime_utr;Parent=Msa1015490-mRNA-1
Gene Sequence

>Msa1015490

ATGAAGAAGAGGAAAACACAAATAGAGAAGAAGAACAAGCGAGTTATCAATGAAGATGTTGTACAGGATGACATAGATAAGGAATTTCAAGAGGAAGACGTATATGATGAATTTTTTGAGGACGAATTTCATGAGGACAACTTAACTGATGAATTTGAGGAGGATGATATAGATGATGAATTACAAGATAAACTTGAATGA
Protein sequence

>Msa1015490

MKKRKTQIEKKNKRVINEDVVQDDIDKEFQEEDVYDEFFEDEFHEDNLTDEFEEDDIDDELQDKLE*